TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2161285.2.1
(780 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO366343.1|CO366343 RTK1_27_G06.b1_A029 Roots minus pota... 122 6e-046
gb|CO363686.1|CO363686 RTK1_11_F01.b1_A029 Roots minus pota... 90 2e-036
gb|DR090575.1|DR090575 RTAL1_16_B07.b1_A029 Roots plus adde... 139 2e-032
gb|DR090649.1|DR090649 RTAL1_16_B07.g1_A029 Roots plus adde... 136 2e-031
gb|DR119509.1|DR119509 RTMG1_23_E07.g1_A029 Roots minus mag... 134 6e-031
gb|AW290680.1|AW290680 NXNV044H05F Nsf Xylem Normal wood Ve... 132 4e-030
gb|CD027338.1|CD027338 NXNV044H05 Nsf Xylem Normal wood Ver... 132 4e-030
gb|DT634133.1|DT634133 EST1149064 Normalized pine embryo li... 116 1e-025
gb|DR687165.1|DR687165 EST1077243 Normalized pine embryo li... 110 2e-023
>gb|CO366343.1|CO366343 RTK1_27_G06.b1_A029 Roots minus potassium Pinus taeda cDNA clone
RTK1_27_G06_A029 3', mRNA sequence
Length = 884
Score = 122 bits (261), Expect(3) = 6e-046
Identities = 47/79 (59%), Positives = 57/79 (72%)
Frame = -3 / +2
Query: 697 NSDIVQMASYAPLFVNDNDQTWNPDAIVFNSWQHYGTPSYWMQTIFRESSGAMIHPVNIX 518
NSD+V+MASYAPLFVN N++ WNPDAIVF+SWQ YGTPSYW+Q FRES+GA++ PV I
Sbjct: 5 NSDVVEMASYAPLFVNVNNRRWNPDAIVFDSWQQYGTPSYWVQQFFRESNGAVLLPVTIQ 184
Query: 517 XXXXXXXXXXAITWQDAEN 461
AI W E+
Sbjct: 185 ENATSALVASAIRWSSLED 241
Score = 69.8 bits (146), Expect(3) = 6e-046
Identities = 27/59 (45%), Positives = 39/59 (66%)
Frame = -3 / +2
Query: 382 SVNALGSTATVLTSGNVMDENSFSNPNKVAPVKSQLSNAAEQMQVTLAPHSFTAFDLAL 206
S+ + GS T+LTS N+MDENSF P K+AP +++ N+ + ++L PHS T DL L
Sbjct: 329 SIRSSGSKKTILTSDNLMDENSFDMPQKIAPKATEIVNSGTDINISLPPHSVTVVDLQL 505
Score = 30.9 bits (61), Expect(3) = 6e-046
Identities = 11/23 (47%), Positives = 18/23 (78%)
Frame = -3 / +2
Query: 457 FLRVKVVNFGSDPVSLTVSTSGL 389
++R+K VNFGS ++L ++ SGL
Sbjct: 251 YIRLKAVNFGSSLLTLDITISGL 319
>gb|CO363686.1|CO363686 RTK1_11_F01.b1_A029 Roots minus potassium Pinus taeda cDNA clone
RTK1_11_F01_A029 3', mRNA sequence
Length = 836
Score = 90.4 bits (191), Expect(3) = 2e-036
Identities = 34/64 (53%), Positives = 42/64 (65%)
Frame = -3 / +2
Query: 652 NDNDQTWNPDAIVFNSWQHYGTPSYWMQTIFRESSGAMIHPVNIXXXXXXXXXXXAITWQ 473
N N++ WNPDAIVF+SWQ YGTPSYW+Q FRES+GA++ PV I AI W
Sbjct: 2 NVNNRRWNPDAIVFDSWQQYGTPSYWVQQFFRESNGAVLLPVTIQENATSALVASAIRWS 181
Query: 472 DAEN 461
E+
Sbjct: 182 SLED 193
Score = 69.8 bits (146), Expect(3) = 2e-036
Identities = 27/59 (45%), Positives = 39/59 (66%)
Frame = -3 / +2
Query: 382 SVNALGSTATVLTSGNVMDENSFSNPNKVAPVKSQLSNAAEQMQVTLAPHSFTAFDLAL 206
S+ + GS T+LTS N+MDENSF P K+AP +++ N+ + ++L PHS T DL L
Sbjct: 281 SIRSSGSKKTILTSDNLMDENSFDMPQKIAPKATEIVNSGTDINISLPPHSVTVVDLQL 457
Score = 30.9 bits (61), Expect(3) = 2e-036
Identities = 11/23 (47%), Positives = 18/23 (78%)
Frame = -3 / +2
Query: 457 FLRVKVVNFGSDPVSLTVSTSGL 389
++R+K VNFGS ++L ++ SGL
Sbjct: 203 YIRLKAVNFGSSLLTLDITISGL 271
>gb|DR090575.1|DR090575 RTAL1_16_B07.b1_A029 Roots plus added aluminum Pinus taeda cDNA
clone RTAL1_16_B07_A029 3', mRNA sequence
Length = 778
Score = 139 bits (298), Expect = 2e-032
Identities = 63/140 (45%), Positives = 80/140 (57%)
Frame = -3 / +1
Query: 763 DAGRGXXXXXXXXXXXLTGLEKNSDIVQMASYAPLFVNDNDQTWNPDAIVFNSWQHYGTP 584
DAG G L GLE NSD+V+MASYAPLFVN N++ WNPDAIVF+SWQ YGTP
Sbjct: 1 DAGHGSLLAAIGEAGFLIGLETNSDVVEMASYAPLFVNVNNRRWNPDAIVFDSWQQYGTP 180
Query: 583 SYWMQTIFRESSGAMIHPVNIXXXXXXXXXXXAITWQDAENSFLRVKVVNFGSDPVSLTV 404
SYW+Q FRESSGA++ PV I AI W E+ ++V LT+
Sbjct: 181 SYWVQQFFRESSGAVLLPVTIQENATSALVASAIRWSSLEDGEDYIRVKAVNFGSSLLTL 360
Query: 403 STSGLGASVNALGSTATVLT 344
+ G V ++ S+ + T
Sbjct: 361 DITISGLPVGSIRSSGSKKT 420
Score = 107 bits (229), Expect(3) = 9e-029
Identities = 53/95 (55%), Positives = 61/95 (64%)
Frame = +3 / -3
Query: 477 HVIADAASELEYRELILTGWIMAPLDSRKIVCIQ*LGVP*CCQELKTIASGFHVWSLSFT 656
H+IA+A I+TG APLDS K C Q*LGVP CC E TIASGFH+ L+ T
Sbjct: 287 HLIAEATKADVAFSCIVTGRSTAPLDSLKNCCTQ*LGVPYCCHESNTIASGFHLRLLTLT 108
Query: 657 KSGA*LAI*TISLFFSRPVRKAASANEARRLPLPA 761
+GA*LAI T SLF S P+RK AS A +LP PA
Sbjct: 107 NNGA*LAISTTSLFVSNPIRKPASPIAANKLPCPA 3
Score = 69.8 bits (146), Expect(3) = 4e-023
Identities = 27/59 (45%), Positives = 39/59 (66%)
Frame = -3 / +1
Query: 382 SVNALGSTATVLTSGNVMDENSFSNPNKVAPVKSQLSNAAEQMQVTLAPHSFTAFDLAL 206
S+ + GS T+LTS N+MDENSF P K+AP +++ N+ + ++L PHS T DL L
Sbjct: 391 SIRSSGSKKTILTSDNLMDENSFDMPQKIAPKATEIVNSGTDINISLPPHSVTVVDLQL 567
Score = 43.7 bits (89), Expect(3) = 4e-023
Identities = 21/49 (42%), Positives = 25/49 (51%)
Frame = -1 / +2
Query: 696 TVILFRWQVTHHSS*TTMTKRGTRTLSSSTPGSTMELLATGCRRFSASQ 550
TV+ RWQ HH +T GT+ L GS M L ATGC S +Q
Sbjct: 68 TVM*LRWQAMHHCLSMLITADGTQMLLYLIHGSNMGLQATGCNNSSGNQ 214
Score = 35.0 bits (70), Expect(3) = 9e-029
Identities = 20/55 (36%), Positives = 26/55 (47%)
Frame = +3 / -3
Query: 204 ASARSKAVKE*GASVTCICSAALLSWLFTGATLFGLLKEFSSMTLPDVRTVAVDP 368
+S +S V E G I + GA G+ KEFSS+ L DVR V +P
Sbjct: 569 SSCKSTTVTECGGREMLISVPLFTISVALGAIFCGMSKEFSSIKLSDVRMVFFEP 405
Score = 32.2 bits (64), Expect(3) = 4e-023
Identities = 12/23 (52%), Positives = 18/23 (78%)
Frame = -3 / +1
Query: 457 FLRVKVVNFGSDPVSLTVSTSGL 389
++RVK VNFGS ++L ++ SGL
Sbjct: 313 YIRVKAVNFGSSLLTLDITISGL 381
Score = 22.1 bits (42), Expect(3) = 9e-029
Identities = 9/20 (45%), Positives = 12/20 (60%)
Frame = +3 / -3
Query: 393 PLVETVRLTGSDPKFTTFTL 452
PL+ ++ PKFT FTL
Sbjct: 377 PLIVMSSVSRELPKFTAFTL 318
>gb|DR090649.1|DR090649 RTAL1_16_B07.g1_A029 Roots plus added aluminum Pinus taeda cDNA
clone RTAL1_16_B07_A029 5', mRNA sequence
Length = 746
Score = 136 bits (292), Expect = 2e-031
Identities = 62/139 (44%), Positives = 79/139 (56%)
Frame = -3 / +3
Query: 760 AGRGXXXXXXXXXXXLTGLEKNSDIVQMASYAPLFVNDNDQTWNPDAIVFNSWQHYGTPS 581
AG G L GLE NSD+V+MASYAPLFVN N++ WNPDAIVF+SWQ YGTPS
Sbjct: 3 AGHGSLLAAIGEAGFLIGLETNSDVVEMASYAPLFVNVNNRRWNPDAIVFDSWQQYGTPS 182
Query: 580 YWMQTIFRESSGAMIHPVNIXXXXXXXXXXXAITWQDAENSFLRVKVVNFGSDPVSLTVS 401
YW+Q FRESSGA++ PV I AI W E+ ++V LT+
Sbjct: 183 YWVQQFFRESSGAVLLPVTIQENATSALVASAIRWSSLEDGEDYIRVKAVNFGSSLLTLD 362
Query: 400 TSGLGASVNALGSTATVLT 344
+ G V ++ S+ + T
Sbjct: 363 ITISGLPVGSIRSSGSKKT 419
Score = 107 bits (229), Expect(3) = 9e-029
Identities = 53/95 (55%), Positives = 61/95 (64%)
Frame = +3 / -2
Query: 477 HVIADAASELEYRELILTGWIMAPLDSRKIVCIQ*LGVP*CCQELKTIASGFHVWSLSFT 656
H+IA+A I+TG APLDS K C Q*LGVP CC E TIASGFH+ L+ T
Sbjct: 286 HLIAEATKADVAFSCIVTGRSTAPLDSLKNCCTQ*LGVPYCCHESNTIASGFHLRLLTLT 107
Query: 657 KSGA*LAI*TISLFFSRPVRKAASANEARRLPLPA 761
+GA*LAI T SLF S P+RK AS A +LP PA
Sbjct: 106 NNGA*LAISTTSLFVSNPIRKPASPIAANKLPCPA 2
Score = 69.8 bits (146), Expect(3) = 4e-023
Identities = 27/59 (45%), Positives = 39/59 (66%)
Frame = -3 / +3
Query: 382 SVNALGSTATVLTSGNVMDENSFSNPNKVAPVKSQLSNAAEQMQVTLAPHSFTAFDLAL 206
S+ + GS T+LTS N+MDENSF P K+AP +++ N+ + ++L PHS T DL L
Sbjct: 390 SIRSSGSKKTILTSDNLMDENSFDMPQKIAPKATEIVNSGTDINISLPPHSVTVVDLQL 566
Score = 43.7 bits (89), Expect(3) = 4e-023
Identities = 21/49 (42%), Positives = 25/49 (51%)
Frame = -1 / +1
Query: 696 TVILFRWQVTHHSS*TTMTKRGTRTLSSSTPGSTMELLATGCRRFSASQ 550
TV+ RWQ HH +T GT+ L GS M L ATGC S +Q
Sbjct: 67 TVM*LRWQAMHHCLSMLITADGTQMLLYLIHGSNMGLQATGCNNSSGNQ 213
Score = 35.0 bits (70), Expect(3) = 9e-029
Identities = 20/55 (36%), Positives = 26/55 (47%)
Frame = +3 / -2
Query: 204 ASARSKAVKE*GASVTCICSAALLSWLFTGATLFGLLKEFSSMTLPDVRTVAVDP 368
+S +S V E G I + GA G+ KEFSS+ L DVR V +P
Sbjct: 568 SSCKSTTVTECGGREMLISVPLFTISVALGAIFCGMSKEFSSIKLSDVRMVFFEP 404
Score = 32.2 bits (64), Expect(3) = 4e-023
Identities = 12/23 (52%), Positives = 18/23 (78%)
Frame = -3 / +3
Query: 457 FLRVKVVNFGSDPVSLTVSTSGL 389
++RVK VNFGS ++L ++ SGL
Sbjct: 312 YIRVKAVNFGSSLLTLDITISGL 380
Score = 22.1 bits (42), Expect(3) = 9e-029
Identities = 9/20 (45%), Positives = 12/20 (60%)
Frame = +3 / -2
Query: 393 PLVETVRLTGSDPKFTTFTL 452
PL+ ++ PKFT FTL
Sbjct: 376 PLIVMSSVSRELPKFTAFTL 317
>gb|DR119509.1|DR119509 RTMG1_23_E07.g1_A029 Roots minus magnesium Pinus taeda cDNA clone
RTMG1_23_E07_A029 5', mRNA sequence
Length = 619
Score = 134 bits (288), Expect = 6e-031
Identities = 56/101 (55%), Positives = 66/101 (65%)
Frame = -3 / +3
Query: 778 AVWRSDAGRGXXXXXXXXXXXLTGLEKNSDIVQMASYAPLFVNDNDQTWNPDAIVFNSWQ 599
AV +DAG G L GLE NSD+V+MASYAPLFVN N++ WNPDAIVF+SWQ
Sbjct: 315 AVTGTDAGHGSLLAAIGEAGFLIGLETNSDVVEMASYAPLFVNVNNRRWNPDAIVFDSWQ 494
Query: 598 HYGTPSYWMQTIFRESSGAMIHPVNIXXXXXXXXXXXAITW 476
YGTPSYW+Q FRES+GA++ PV I AI W
Sbjct: 495 QYGTPSYWVQQFFRESNGAVLLPVTIQENATSALVASAIRW 617
Score = 110 bits (234), Expect = 2e-023
Identities = 55/101 (54%), Positives = 64/101 (63%)
Frame = +3 / -1
Query: 477 HVIADAASELEYRELILTGWIMAPLDSRKIVCIQ*LGVP*CCQELKTIASGFHVWSLSFT 656
H+IA+A I+TG AP DS K C Q*LGVP CC E TIASGFH+ L+ T
Sbjct: 616 HLIAEATKADVAFSCIVTGRSTAPFDSLKNCCTQ*LGVPYCCHESNTIASGFHLRLLTLT 437
Query: 657 KSGA*LAI*TISLFFSRPVRKAASANEARRLPLPASLLQTA 779
+GA*LAI T SLF S P+RK AS A +LP PAS+ TA
Sbjct: 436 NNGA*LAISTTSLFVSNPIRKPASPIAANKLPCPASVPVTA 314
>gb|AW290680.1|AW290680 NXNV044H05F Nsf Xylem Normal wood Vertical Pinus taeda cDNA clone
NXNV044H05 5', mRNA sequence
Length = 335
Score = 132 bits (282), Expect = 4e-030
Identities = 54/86 (62%), Positives = 63/86 (73%)
Frame = -3 / +2
Query: 778 AVWRSDAGRGXXXXXXXXXXXLTGLEKNSDIVQMASYAPLFVNDNDQTWNPDAIVFNSWQ 599
AV +DAG G L GLE NSD+V+MASYAPLFVN N++ WNPDAIVF+SWQ
Sbjct: 26 AVTGTDAGHGSLLAAIGEAGFLIGLETNSDVVEMASYAPLFVNVNNRRWNPDAIVFDSWQ 205
Query: 598 HYGTPSYWMQTIFRESSGAMIHPVNI 521
YGTPSYW+Q FRESSGA++ PV I
Sbjct: 206 QYGTPSYWVQQFFRESSGAVLLPVTI 283
Score = 108 bits (230), Expect = 6e-023
Identities = 52/86 (60%), Positives = 60/86 (69%)
Frame = +3 / -3
Query: 522 ILTGWIMAPLDSRKIVCIQ*LGVP*CCQELKTIASGFHVWSLSFTKSGA*LAI*TISLFF 701
I+TG APLDS K C Q*LGVP CC E TIASGFH+ L+ T +GA*LAI T SLF
Sbjct: 282 IVTGRSTAPLDSLKNCCTQ*LGVPYCCHESNTIASGFHLRLLTLTNNGA*LAISTTSLFV 103
Query: 702 SRPVRKAASANEARRLPLPASLLQTA 779
S+P+RK AS A +LP PAS+ TA
Sbjct: 102 SKPIRKPASPIAANKLPCPASVPVTA 25
>gb|CD027338.1|CD027338 NXNV044H05 Nsf Xylem Normal wood Vertical Pinus taeda cDNA clone
NXNV044H05 5' similar to Arabidopsis thaliana sequence
At3g10740 putative alpha-L-arabinofuranosidase see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 335
Score = 132 bits (282), Expect = 4e-030
Identities = 54/86 (62%), Positives = 63/86 (73%)
Frame = -3 / +2
Query: 778 AVWRSDAGRGXXXXXXXXXXXLTGLEKNSDIVQMASYAPLFVNDNDQTWNPDAIVFNSWQ 599
AV +DAG G L GLE NSD+V+MASYAPLFVN N++ WNPDAIVF+SWQ
Sbjct: 26 AVTGTDAGHGSLLAAIGEAGFLIGLETNSDVVEMASYAPLFVNVNNRRWNPDAIVFDSWQ 205
Query: 598 HYGTPSYWMQTIFRESSGAMIHPVNI 521
YGTPSYW+Q FRESSGA++ PV I
Sbjct: 206 QYGTPSYWVQQFFRESSGAVLLPVTI 283
Score = 108 bits (230), Expect = 6e-023
Identities = 52/86 (60%), Positives = 60/86 (69%)
Frame = +3 / -3
Query: 522 ILTGWIMAPLDSRKIVCIQ*LGVP*CCQELKTIASGFHVWSLSFTKSGA*LAI*TISLFF 701
I+TG APLDS K C Q*LGVP CC E TIASGFH+ L+ T +GA*LAI T SLF
Sbjct: 282 IVTGRSTAPLDSLKNCCTQ*LGVPYCCHESNTIASGFHLRLLTLTNNGA*LAISTTSLFV 103
Query: 702 SRPVRKAASANEARRLPLPASLLQTA 779
S+P+RK AS A +LP PAS+ TA
Sbjct: 102 SKPIRKPASPIAANKLPCPASVPVTA 25
>gb|DT634133.1|DT634133 EST1149064 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMG262 3' end, mRNA sequence
Length = 803
Score = 116 bits (249), Expect = 1e-025
Identities = 47/75 (62%), Positives = 54/75 (72%)
Frame = -3 / +2
Query: 778 AVWRSDAGRGXXXXXXXXXXXLTGLEKNSDIVQMASYAPLFVNDNDQTWNPDAIVFNSWQ 599
AV +DAG G L GLE NSD+V+MASYAPLFVN N++ WNPDAIVF+SWQ
Sbjct: 578 AVTGTDAGHGSLLAAIGEAGFLIGLETNSDVVEMASYAPLFVNVNNRRWNPDAIVFDSWQ 757
Query: 598 HYGTPSYWMQTIFRE 554
YGTPSYW+Q FRE
Sbjct: 758 QYGTPSYWVQQFFRE 802
>gb|DR687165.1|DR687165 EST1077243 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWABO01 3' end, mRNA sequence
Length = 784
Score = 110 bits (234), Expect = 2e-023
Identities = 44/70 (62%), Positives = 51/70 (72%)
Frame = -3 / +3
Query: 778 AVWRSDAGRGXXXXXXXXXXXLTGLEKNSDIVQMASYAPLFVNDNDQTWNPDAIVFNSWQ 599
AV +DAG G L GLE NSD+V+MASYAPLFVN N++ WNPDAIVF+SWQ
Sbjct: 570 AVTGTDAGHGSLLAAIGEAGFLIGLETNSDVVEMASYAPLFVNVNNRRWNPDAIVFDSWQ 749
Query: 598 HYGTPSYWMQ 569
YGTPSYW+Q
Sbjct: 750 QYGTPSYWVQ 779
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 413,075,935
Number of Sequences: 355925
Number of extensions: 5834504
Number of successful extensions: 50061
Number of sequences better than 1.0e-020: 11
length of database: 72,425,745
effective HSP length: 52
effective length of database: 53,917,645
effective search space used: 11160952515
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)