TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCS19f07.yg.2.1
(626 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF636584.1|BF636584 NF090F03DT1F1030 Drought Medicago tr... 354 6e-097
gb|AC144483.10| Medicago truncatula clone mth2-12f13, compl... 150 5e-082
gb|CB894037.1|CB894037 EST646829 HOGA Medicago truncatula c... 205 6e-052
gb|CF069019.1|CF069019 EST669740 MTUS Medicago truncatula c... 145 9e-050
gb|CX532799.1|CX532799 s13dNF71G04MJ024_271881 Methyl Jasmo... 141 2e-049
gb|AC140023.13| Medicago truncatula clone mth2-32h4, comple... 109 5e-048
gb|BE325221.2|BE325221 NF114H07ST1F1063 Developing stem Med... 113 4e-041
gb|AW690130.1|AW690130 NF028B12ST1F1000 Developing stem Med... 110 3e-040
gb|CX527554.1|CX527554 s13dNF43H03AT032_515620 Aphid-Infect... 144 2e-033
gb|BI308510.1|BI308510 EST529920 GPOD Medicago truncatula c... 88 2e-032
gb|AW688927.1|AW688927 NF013C11ST1F1000 Developing stem Med... 85 2e-032
gb|AC151529.20| Medicago truncatula clone mth2-7n23, comple... 60 7e-032
gb|AC144478.20| Medicago truncatula clone mth2-20b20, compl... 60 3e-031
gb|BG646172.1|BG646172 EST507791 KV3 Medicago truncatula cD... 135 5e-031
gb|AC127428.38| Medicago truncatula clone mth2-36c19, WORKI... 60 4e-030
gb|AW689134.1|AW689134 NF015G05ST1F1000 Developing stem Med... 120 2e-027
gb|CX534173.1|CX534173 s13dNF0TC01MJ002_322105 Methyl Jasmo... 113 3e-024
gb|BI308096.1|BI308096 EST529506 GPOD Medicago truncatula c... 109 5e-023
gb|CX540580.1|CX540580 s13dNF0CC09GS070_464436 Germinating ... 103 3e-021
>gb|BF636584.1|BF636584 NF090F03DT1F1030 Drought Medicago truncatula cDNA clone NF090F03DT
5', mRNA sequence
Length = 627
Score = 354 bits (768), Expect = 6e-097
Identities = 135/170 (79%), Positives = 151/170 (88%)
Frame = -1 / +3
Query: 560 AANSXPFTIFYNPRAXPSEFVIPLAKYNKALYTQVSLGMRFRMLFETEDSGVRRYMGTIT 381
A+N+ PFTIFYNPR P EFVIPLAKYNKALYT VSLGMRFRM+FETEDSGVRRYMGTIT
Sbjct: 75 ASNNSPFTIFYNPRTSPCEFVIPLAKYNKALYTHVSLGMRFRMMFETEDSGVRRYMGTIT 254
Query: 380 GIGDLDPLRWKNSHWRNLQVGWDESTASERRTRVSIWEIEPVATPFYICPPPFFRPKLPK 201
GI DLDP+RWKNS WRNLQVGWDESTA ER +RVSIW+IEPV TPFYICPPPFFRPK PK
Sbjct: 255 GISDLDPVRWKNSQWRNLQVGWDESTAGERPSRVSIWDIEPVVTPFYICPPPFFRPKFPK 434
Query: 200 QAGMPDDENEVESAFKRAMPWLADDFALKDVQNALFPGLSLVQWMAMQQN 51
Q GMP+DE+E+E+AFKRAMPWL D+ +KD +++FPGLSLVQWM+M N
Sbjct: 435 QPGMPEDESEIENAFKRAMPWLGDELGMKDTSSSVFPGLSLVQWMSMXXN 584
Score = 244 bits (527), Expect = 1e-063
Identities = 112/166 (67%), Positives = 123/166 (74%)
Frame = +1 / -3
Query: 58 CIAIH*TRLRPGNNAFWTSFRAKSSASHGIALLKALSTSFSSSGIPACLGSFGLKNGGGH 237
CI IH T+L+PG SF SS SHGIALL A S S SSSGIP C G+FGLKNGGG
Sbjct: 577 CILIHCTKLKPGKTELEVSFMPSSSPSHGIALLNAFSISDSSSGIPGCFGNFGLKNGGGQ 398
Query: 238 I*NGVATGSISHIETRVRLSDAVDSSQPT*RFRQ*EFFHRSGSKSPMPVIVPMYLLTPES 417
+* GV TGS+S IET + S AVDSS PT*R R EFF R+GSKS +PVIVP+YL TPES
Sbjct: 397 M*KGVTTGSMSQIETLLGRSPAVDSSHPT*RLRHCEFFQRTGSKSLIPVIVPIYLRTPES 218
Query: 418 SVSKSILNRIPRDTCVYRALLYFAKGMTNSDGXALGL*KIVNGLLF 555
SVS ILN IPR+T VYRALLY A G+TNS G LGL* IVNGLLF
Sbjct: 217 SVSNIILNLIPRET*VYRALLYLANGITNSQGLVLGL*NIVNGLLF 80
Score = 175 bits (377), Expect = 5e-043
Identities = 74/150 (49%), Positives = 96/150 (64%)
Frame = +3 / -2
Query: 111 IFQSEVISKPWHRSFESTLNLVFIIWHSCLLRKLWPEKWWWAYIKWCSNWFNLPYRNTGA 290
I +E ISKPWH S E N I WHS LLR+LWP+K W ++ NWFN+P N+
Sbjct: 524 ILHAEFISKPWHSSLECIFNFRLIFWHSWLLRELWPKKRRWTNVERSHNWFNVPN*NSTW 345
Query: 291 PLRCSRFIPTNLKVSPMRILPSQWI*VANACDRAHVSSYP*ILCLKEHSESHSKGHLCIQ 470
L F+P+NLK++P+ I P+ I V + D AH+S+Y *ILCLK HSESHSKG++ +Q
Sbjct: 344 ALTSC*FVPSNLKIAPL*IFPADRIQVTDPSDCAHISAYS*ILCLKHHSESHSKGNMSVQ 165
Query: 471 SLIVFC*RDDKF*WACPWIIENSKWXTICS 560
S I+ + KF A PWII+ SKW I S
Sbjct: 164 SFIILGQWNHKFTGASPWIIKYSKWTVIRS 75
Score = 135 bits (289), Expect = 7e-031
Identities = 75/171 (43%), Positives = 96/171 (56%)
Frame = -2 / +1
Query: 562 LLQIVXHLLFSIIQGXAHQNLSSL*QNTIRLCIHKCPLECDSECSLRQRIQG*EDTWARS 383
LL+I HLL+ IIQG A NL + I+LC PLE DSE LRQRIQ D WA+S
Sbjct: 73 LLRITVHLLYFIIQGLAPVNL*FHWPSIIKLCTLMFPLE*DSE*CLRQRIQEYADIWAQS 252
Query: 382 QALAT*IHCDGRILIGETFRLVGMNRLHLRGAPVFRYGRLNQLLHHFIYAHHHFSGQSFL 203
T I G+I G FRL G N+ + F++G LNQL + H F GQS
Sbjct: 253 LGSVTWILSAGKIHNGAIFRLDGTNQQLVSAQVEFQFGTLNQL*LLSTFVHLRFLGQSSR 432
Query: 202 SKQECQMMKTRLRVLSKERCHGLLMTSL*KMSKMHYFQA*A*FNGWLCSKI 50
S QECQ M +L++ S+E CHGL M S *++ ++ * *+NG +C I
Sbjct: 433 SNQECQKMSLKLKMHSRELCHGLEMNSA*RIPQVQSSLV*V*YNG*VCXXI 585
Score = 112 bits (240), Expect(2) = 1e-025
Identities = 51/114 (44%), Positives = 75/114 (65%)
Frame = -3 / +2
Query: 561 CCK**XIYYFL*SKGXPIRICHPFSKIQ*GSVYTSVPWNAIQNAL*DRGFRGKKIHGHDH 382
C +* IYY L*SK P+ IC ++ * SV++ PWN IQN +*DRGFR +I+GH+H
Sbjct: 74 CFE*QSIYYIL*SKD*PL*ICDSIGQV**SSVHSCFPWNEIQNDV*DRGFRSTQIYGHNH 253
Query: 381 RHWRLRSTAMEEFSLAKPSGWLG*IDCI*EAHPCFDMGD*TSCYTILYMPTTIF 220
L S +E+F++A+ SGW+G I+ * F++G *TSC + L++ T++F
Sbjct: 254 WDQ*LGSCPLEKFTMAQSSGWMGRINSW*APK*SFNLGH*TSCDSFLHLSTSVF 415
Score = 24.9 bits (48), Expect(2) = 1e-025
Identities = 17/37 (45%), Positives = 21/37 (56%)
Frame = -3 / +2
Query: 168 *ECFQKSDAMAC**LRSERCPKCIISRPEPSSMDGYA 58
*+C Q+S AMA * R E K + E S+MD YA
Sbjct: 467 *KCIQESYAMAWR*TRHEGYLKFSLPWFEFSTMDEYA 577
>gb|AC144483.10| Medicago truncatula clone mth2-12f13, complete sequence
Length = 105599
Score = 150 bits (323), Expect(4) = 5e-082
Identities = 58/67 (86%), Positives = 64/67 (95%)
Frame = -1 / -3
Query: 521 RAXPSEFVIPLAKYNKALYTQVSLGMRFRMLFETEDSGVRRYMGTITGIGDLDPLRWKNS 342
RA PSEFV+PLAKYNKA+YTQVSLGMRFRM+FETE+SGVRRYMGT+TGI DLDP+RWKNS
Sbjct: 51054 RASPSEFVVPLAKYNKAMYTQVSLGMRFRMMFETEESGVRRYMGTVTGISDLDPVRWKNS 50875
Query: 341 HWRNLQV 321
WRNLQV
Sbjct: 50874 QWRNLQV 50854
Score = 104 bits (222), Expect(4) = 7e-044
Identities = 47/67 (70%), Positives = 52/67 (77%)
Frame = +1 / +2
Query: 322 T*RFRQ*EFFHRSGSKSPMPVIVPMYLLTPESSVSKSILNRIPRDTCVYRALLYFAKGMT 501
T*R R EFFHR+GSKS +PV VP+YL TP+SSVS ILN IPRDT VY ALLY A G T
Sbjct: 50855 T*RLRHCEFFHRTGSKSLIPVTVPIYLRTPDSSVSNIILNLIPRDT*VYMALLYLANGTT 51034
Query: 502 NSDGXAL 522
NS+G AL
Sbjct: 51035 NSEGLAL 51055
Score = 99.6 bits (211), Expect(4) = 5e-082
Identities = 37/52 (71%), Positives = 41/52 (78%)
Frame = -1 / -3
Query: 329 LQVGWDESTASERRTRVSIWEIEPVATPFYICPPPFFRPKLPKQAGMPDDEN 174
LQVGWDESTA ER +RVSIW+IEPV TPFYICPPPFFR P GMP ++
Sbjct: 50766 LQVGWDESTAGERPSRVSIWDIEPVVTPFYICPPPFFRQNFPGHPGMPGSKS 50611
Score = 85.4 bits (180), Expect(4) = 5e-082
Identities = 31/47 (65%), Positives = 40/47 (85%)
Frame = -1 / -2
Query: 185 DDENEVESAFKRAMPWLADDFALKDVQNALFPGLSLVQWMAMQQNPQ 45
DD ++VE++FKRAMPWL DDF +KD +++FPGLSLVQWM+MQQ Q
Sbjct: 50461 DDGSDVENSFKRAMPWLGDDFGMKDASSSVFPGLSLVQWMSMQQKNQ 50321
Score = 68.4 bits (143), Expect(3) = 1e-021
Identities = 28/66 (42%), Positives = 42/66 (63%)
Frame = +3 / +1
Query: 324 LKVSPMRILPSQWI*VANACDRAHVSSYP*ILCLKEHSESHSKGHLCIQSLIVFC*RDDK 503
LK++P+ I PS I V N+C+ H+S+Y +LC K HS+SHSK HL + ++ ++K
Sbjct: 50857 LKIAPL*IFPSDRIQVTNSCNCTHISTYSRLLCFKHHSKSHSKRHLSVHGFVILGQWNNK 51036
Query: 504 F*WACP 521
F A P
Sbjct: 51037 FGGAGP 51054
Score = 62.0 bits (129), Expect(4) = 7e-044
Identities = 29/46 (63%), Positives = 31/46 (67%)
Frame = +1 / +2
Query: 187 GIPACLGSFGLKNGGGHI*NGVATGSISHIETRVRLSDAVDSSQPT 324
G+P G F LK GGG I* GV TGSIS IET + S AVDSS PT
Sbjct: 50624 GMPGWPGKFCLKKGGGQI*KGVTTGSISQIETLLGRSPAVDSSHPT 50761
Score = 58.3 bits (121), Expect(4) = 7e-044
Identities = 26/45 (57%), Positives = 29/45 (64%)
Frame = +1 / +3
Query: 52 FCCIAIH*TRLRPGNNAFWTSFRAKSSASHGIALLKALSTSFSSS 186
FCC+ IH T+L+PG SF KSS S GIALLK STS SS
Sbjct: 50328 FCCMLIHCTKLKPGKTELEASFMPKSSPSQGIALLKEFSTSDPSS 50462
Score = 51.9 bits (107), Expect(4) = 5e-082
Identities = 22/36 (61%), Positives = 24/36 (66%)
Frame = -1 / -3
Query: 626 LSSSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPR 519
LSSS S M I AA HAAAN+ PFTI+YNPR
Sbjct: 51258 LSSSVISSDSMHIGILAAAAHAAANNSPFTIYYNPR 51151
Score = 48.3 bits (99), Expect(3) = 1e-021
Identities = 19/47 (40%), Positives = 27/47 (57%)
Frame = +3 / +1
Query: 186 WHSCLLRKLWPEKWWWAYIKWCSNWFNLPYRNTGAPLRCSRFIPTNL 326
WH+ + R++ PEK W I+ WFN+P N+ RFIP+NL
Sbjct: 50623 WHARVAREVLPEKRRWTNIERSHYWFNIPN*NSAWAFTSCRFIPSNL 50763
Score = 33.6 bits (67), Expect(4) = 7e-044
Identities = 12/26 (46%), Positives = 15/26 (57%)
Frame = +3 / +1
Query: 519 PWIIENSKWXTICSSMGXCCXDSXXH 596
PWI+ SKW ICSSM ++ H
Sbjct: 51151 PWIVIYSKWTVICSSMSGSS*NANMH 51228
Score = 24.4 bits (47), Expect(3) = 1e-021
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = +1 / +2
Query: 520 LGL*KIVNGLLF 555
LGL* IVNGLLF
Sbjct: 51152 LGL*YIVNGLLF 51187
>gb|CB894037.1|CB894037 EST646829 HOGA Medicago truncatula cDNA clone HOGA-29H14, mRNA
sequence
Length = 833
Score = 205 bits (442), Expect = 6e-052
Identities = 83/105 (79%), Positives = 87/105 (82%)
Frame = -1 / +3
Query: 626 LSSSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPRAXPSEFVIPLAKYNKALYTQVSLG 447
LSSS S M I AA HAA+N+ PFTIFYNPR P EFVIPLAKYNKALYT VSLG
Sbjct: 519 LSSSVISSDSMHIGILAAAAHAASNNSPFTIFYNPRTSPCEFVIPLAKYNKALYTHVSLG 698
Query: 446 MRFRMLFETEDSGVRRYMGTITGIGDLDPLRWKNSHWRNLQVGWD 312
MRFRM+FETEDSGVRRYMGTITGI DLDP+RWKNS WRNLQVGWD
Sbjct: 699 MRFRMMFETEDSGVRRYMGTITGISDLDPVRWKNSQWRNLQVGWD 833
Score = 133 bits (284), Expect = 3e-030
Identities = 63/104 (60%), Positives = 69/104 (66%)
Frame = +1 / -2
Query: 313 SQPT*RFRQ*EFFHRSGSKSPMPVIVPMYLLTPESSVSKSILNRIPRDTCVYRALLYFAK 492
S PT*R R EFF R+GSKS +PVIVP+YL TPESSVS ILN IPR+T VYRALLY A
Sbjct: 832 SHPT*RLRHCEFFQRTGSKSLIPVIVPIYLRTPESSVSNIILNLIPRET*VYRALLYLAN 653
Query: 493 GMTNSDGXALGL*KIVNGLLFXXXXXXXXXIXXXMXXXXXXDDD 624
G+TNS G LGL* IVNGLLF I M D+D
Sbjct: 652 GITNSQGLVLGL*NIVNGLLFEAA*AAAARIPICMLSLDITDED 521
Score = 106 bits (227), Expect = 3e-022
Identities = 46/95 (48%), Positives = 62/95 (65%)
Frame = +3 / -1
Query: 312 IPTNLKVSPMRILPSQWI*VANACDRAHVSSYP*ILCLKEHSESHSKGHLCIQSLIVFC* 491
+P+NLK++P+ I P+ I V + D AH+S+Y *ILCLK HSESHSKG++ +QS I+
Sbjct: 833 VPSNLKIAPL*IFPADRIQVTDPSDCAHISAYS*ILCLKHHSESHSKGNMSVQSFIILGQ 654
Query: 492 RDDKF*WACPWIIENSKWXTICSSMGXCCXDSXXH 596
+ KF A PWII+ SKW I SSM + H
Sbjct: 653 WNHKFTGASPWIIKYSKWTVIRSSMSRSSKNPDMH 549
>gb|CF069019.1|CF069019 EST669740 MTUS Medicago truncatula cDNA clone MTUS-15C12, mRNA
sequence
Length = 547
Score = 145 bits (312), Expect(2) = 9e-050
Identities = 54/73 (73%), Positives = 66/73 (90%)
Frame = -1 / +2
Query: 467 YTQVSLGMRFRMLFETEDSGVRRYMGTITGIGDLDPLRWKNSHWRNLQVGWDESTASERR 288
+T+VS+GMRFRMLFETE+S VRRYMGTIT I D+DP+RW NSHWR+++VGWDESTA ER+
Sbjct: 299 HTRVSVGMRFRMLFETEESSVRRYMGTITSISDMDPVRWPNSHWRSVKVGWDESTAGERQ 478
Query: 287 TRVSIWEIEPVAT 249
RVS+WEIEP+ T
Sbjct: 479 PRVSLWEIEPLTT 517
Score = 97.3 bits (206), Expect(2) = 8e-027
Identities = 50/79 (63%), Positives = 56/79 (70%)
Frame = +1 / -2
Query: 250 VATGSISHIETRVRLSDAVDSSQPT*RFRQ*EFFHRSGSKSPMPVIVPMYLLTPESSVSK 429
V GSISH +TR LS AVDSSQPT Q*EF HR+GS S M VIVPMYL T +SSVSK
Sbjct: 516 VVKGSISHNDTRGCLSPAVDSSQPTLTDLQ*EFGHRTGSMSLMLVIVPMYLRTLDSSVSK 337
Query: 430 SILNRIPRDTCVYRALLYF 486
SIL RIP +T V+ L +
Sbjct: 336 SILKRIPTETRVWYTALIY 280
Score = 72.5 bits (152), Expect(2) = 9e-050
Identities = 30/54 (55%), Positives = 37/54 (68%)
Frame = -1 / +2
Query: 626 LSSSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPRAXPSEFVIPLAKYNKALY 465
+ SS S M + AA HAAA + FT+F+NPRA PSEFVIPL+KY KA+Y
Sbjct: 137 MPSSVLSSDSMHIGLLAAAAHAAATNSCFTVFFNPRASPSEFVIPLSKYIKAVY 298
Score = 44.1 bits (90), Expect(2) = 8e-027
Identities = 22/52 (42%), Positives = 23/52 (44%)
Frame = +1 / -2
Query: 466 YRALLYFAKGMTNSDGXALGL*KIVNGLLFXXXXXXXXXIXXXMXXXXXXDD 621
Y AL+YF GMTNSDG ALGL V LL M DD
Sbjct: 297 YTALIYFESGMTNSDGLALGLKNTVKQLLVAAACAAAARSPMCMLSLERTDD 142
>gb|CX532799.1|CX532799 s13dNF71G04MJ024_271881 Methyl Jasmonate-Elicited Root Cell
Suspension Culture Medicago truncatula cDNA, mRNA
sequence
Length = 655
Score = 141 bits (303), Expect(2) = 2e-049
Identities = 54/73 (73%), Positives = 65/73 (89%)
Frame = -1 / +3
Query: 467 YTQVSLGMRFRMLFETEDSGVRRYMGTITGIGDLDPLRWKNSHWRNLQVGWDESTASERR 288
+T+VS+GMRFRMLFETE+S VRRYMGTITGI DLD +RW NSHWR+++VGWDESTA ER+
Sbjct: 426 HTRVSVGMRFRMLFETEESSVRRYMGTITGICDLDSVRWPNSHWRSVKVGWDESTAGERQ 605
Query: 287 TRVSIWEIEPVAT 249
VS+WEIEP+ T
Sbjct: 606 PXVSLWEIEPLTT 644
Score = 91.3 bits (193), Expect(2) = 6e-027
Identities = 46/72 (63%), Positives = 51/72 (70%)
Frame = +1 / -1
Query: 250 VATGSISHIETRVRLSDAVDSSQPT*RFRQ*EFFHRSGSKSPMPVIVPMYLLTPESSVSK 429
V GSISH +T LS AVDSSQPT RQ E HR+ S+S MPVIVPMY T +SSVSK
Sbjct: 643 VVNGSISHKDTXGCLSPAVDSSQPTFTERQCELGHRTESRSQMPVIVPMYRRTLDSSVSK 464
Query: 430 SILNRIPRDTCV 465
SIL RIP +T V
Sbjct: 463 SILKRIPTETRV 428
Score = 75.8 bits (159), Expect(2) = 2e-049
Identities = 33/54 (61%), Positives = 37/54 (68%)
Frame = -1 / +3
Query: 626 LSSSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPRAXPSEFVIPLAKYNKALY 465
+ SS S M + AA HAAA + FTIFYNPRA PSEFVIPLAKY KA+Y
Sbjct: 264 MPSSVLSSDSMHLGLLAAAAHAAATNSRFTIFYNPRACPSEFVIPLAKYVKAVY 425
Score = 50.6 bits (104), Expect(2) = 6e-027
Identities = 23/29 (79%), Positives = 23/29 (79%)
Frame = +1 / -1
Query: 466 YRALLYFAKGMTNSDGXALGL*KIVNGLL 552
Y AL YFAKGMTNSDG A GL*KIV LL
Sbjct: 424 YTALTYFAKGMTNSDGHARGL*KIVKRLL 338
>gb|AC140023.13| Medicago truncatula clone mth2-32h4, complete sequence
Length = 152468
Score = 109 bits (233), Expect(6) = 5e-048
Identities = 39/50 (78%), Positives = 45/50 (90%)
Frame = -1 / +3
Query: 335 RNLQVGWDESTASERRTRVSIWEIEPVATPFYICPPPFFRPKLPKQAGMP 186
+N+QVGWDE+TA ERR+RVSIWEIEPV PF+ICPPPFFRPK P+Q GMP
Sbjct: 130161 KNVQVGWDEATAGERRSRVSIWEIEPVTAPFFICPPPFFRPKRPRQPGMP 130310
Score = 51.9 bits (107), Expect(6) = 5e-048
Identities = 21/37 (56%), Positives = 25/37 (67%)
Frame = -1 / +3
Query: 626 LSSSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPRA 516
LSSS S M I AA HA+AN+ PFT+FYNPR+
Sbjct: 129303 LSSSVLSSDSMHIGILAAAAHASANNSPFTVFYNPRS 129413
Score = 44.6 bits (91), Expect(6) = 5e-048
Identities = 18/28 (64%), Positives = 20/28 (71%)
Frame = -1 / +1
Query: 482 YNKALYTQVSLGMRFRMLFETEDSGVRR 399
Y Q+S GMRFRM+FETEDSG RR
Sbjct: 129574 YRAVYSHQISPGMRFRMMFETEDSGTRR 129657
Score = 41.8 bits (85), Expect(6) = 5e-048
Identities = 16/24 (66%), Positives = 20/24 (83%)
Frame = -1 / +1
Query: 533 FYNPRAXPSEFVIPLAKYNKALYT 462
F+ RA PSEFVIPLAKY +A+Y+
Sbjct: 129520 FFTCRASPSEFVIPLAKYYRAVYS 129591
Score = 38.2 bits (77), Expect(6) = 5e-048
Identities = 13/19 (68%), Positives = 16/19 (84%)
Frame = -1 / +3
Query: 95 FPGLSLVQWMAMQQNPQML 39
FPG+SL QWM +QQNP M+
Sbjct: 130539 FPGMSLAQWMNIQQNPAMV 130595
Score = 35.9 bits (72), Expect(6) = 5e-048
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -1 / +3
Query: 185 DDENEVESAFKRAMPWLADDFALKDVQ 105
D+ + + FK MPWL DD +KD Q
Sbjct: 130455 DESFDFSNLFKNTMPWLGDDMCMKDPQ 130535
>gb|BE325221.2|BE325221 NF114H07ST1F1063 Developing stem Medicago truncatula cDNA clone
NF114H07ST 5', mRNA sequence
Length = 392
Score = 113 bits (242), Expect(2) = 4e-041
Identities = 43/55 (78%), Positives = 51/55 (92%)
Frame = -1 / +2
Query: 467 YTQVSLGMRFRMLFETEDSGVRRYMGTITGIGDLDPLRWKNSHWRNLQVGWDEST 303
+T+VS+GMRFRMLFETE+S VRRYMGTITGI DLD +RW NSHWR+++VGWDEST
Sbjct: 224 HTRVSVGMRFRMLFETEESSVRRYMGTITGICDLDSVRWPNSHWRSVKVGWDEST 388
Score = 75.8 bits (159), Expect(2) = 4e-041
Identities = 33/54 (61%), Positives = 37/54 (68%)
Frame = -1 / +2
Query: 626 LSSSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPRAXPSEFVIPLAKYNKALY 465
+ SS S M + AA HAAA + FTIFYNPRA PSEFVIPLAKY KA+Y
Sbjct: 62 MPSSVLSSDSMHLGLLAAAAHAAATNSRFTIFYNPRACPSEFVIPLAKYVKAVY 223
Score = 73.9 bits (155), Expect(2) = 9e-022
Identities = 36/54 (66%), Positives = 40/54 (74%)
Frame = +1 / -3
Query: 304 VDSSQPT*RFRQ*EFFHRSGSKSPMPVIVPMYLLTPESSVSKSILNRIPRDTCV 465
VDSSQPT RQ E HR+ S+S MPVIVPMY T +SSVSKSIL RIP +T V
Sbjct: 387 VDSSQPTFTERQCELGHRTESRSQMPVIVPMYRRTLDSSVSKSILKRIPTETRV 226
Score = 50.6 bits (104), Expect(2) = 9e-022
Identities = 23/29 (79%), Positives = 23/29 (79%)
Frame = +1 / -3
Query: 466 YRALLYFAKGMTNSDGXALGL*KIVNGLL 552
Y AL YFAKGMTNSDG A GL*KIV LL
Sbjct: 222 YTALTYFAKGMTNSDGHARGL*KIVKRLL 136
>gb|AW690130.1|AW690130 NF028B12ST1F1000 Developing stem Medicago truncatula cDNA clone
NF028B12ST 5', mRNA sequence
Length = 680
Score = 110 bits (235), Expect(2) = 3e-040
Identities = 42/56 (75%), Positives = 51/56 (91%)
Frame = -1 / +3
Query: 467 YTQVSLGMRFRMLFETEDSGVRRYMGTITGIGDLDPLRWKNSHWRNLQVGWDESTA 300
+T+VS+GMRF MLFETE+S VRRYMGTITGI DL+ +RW NSHWR+++VGWDESTA
Sbjct: 510 HTRVSVGMRFXMLFETEESSVRRYMGTITGICDLNSVRWPNSHWRSVKVGWDESTA 677
Score = 75.8 bits (159), Expect(2) = 3e-040
Identities = 33/54 (61%), Positives = 37/54 (68%)
Frame = -1 / +3
Query: 626 LSSSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPRAXPSEFVIPLAKYNKALY 465
+ SS S M + AA HAAA + FTIFYNPRA PSEFVIPLAKY KA+Y
Sbjct: 348 MPSSVLSSDSMHLGLLAAAAHAAATNSRFTIFYNPRACPSEFVIPLAKYVKAVY 509
Score = 70.7 bits (148), Expect(2) = 8e-021
Identities = 35/55 (63%), Positives = 39/55 (70%)
Frame = +1 / -2
Query: 301 AVDSSQPT*RFRQ*EFFHRSGSKSPMPVIVPMYLLTPESSVSKSILNRIPRDTCV 465
AVDSSQPT RQ E HR+ +S MPVIVPMY T +SSVSKSI RIP +T V
Sbjct: 676 AVDSSQPTFTERQCELGHRTEFRSQMPVIVPMYRRTLDSSVSKSIXKRIPTETRV 512
Score = 50.6 bits (104), Expect(2) = 8e-021
Identities = 23/29 (79%), Positives = 23/29 (79%)
Frame = +1 / -2
Query: 466 YRALLYFAKGMTNSDGXALGL*KIVNGLL 552
Y AL YFAKGMTNSDG A GL*KIV LL
Sbjct: 508 YTALTYFAKGMTNSDGHARGL*KIVKRLL 422
>gb|CX527554.1|CX527554 s13dNF43H03AT032_515620 Aphid-Infected Shoots Medicago truncatula
cDNA, mRNA sequence
Length = 610
Score = 144 bits (308), Expect = 2e-033
Identities = 60/145 (41%), Positives = 83/145 (57%)
Frame = -1 / +2
Query: 620 SSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPRAXPSEFVIPLAKYNKALYTQVSLGMR 441
SS S M + A HA FT++Y PR P+EF++P +Y ++L ++GMR
Sbjct: 62 SSVISSHSMHLGVLATAWHAVLTGTMFTVYYKPRTSPAEFIVPYDQYMESLKNNYTIGMR 241
Query: 440 FRMLFETEDSGVRRYMGTITGIGDLDPLRWKNSHWRNLQVGWDESTASERRTRVSIWEIE 261
F+M FE E++ +R+ GTI GI D D RW S WR L+V WDE++ R RVS W+IE
Sbjct: 242 FKMRFEGEEAPEQRFTGTIVGIEDSDSKRWPTSKWRCLKVRWDETSNIPRPERVSPWKIE 421
Query: 260 PVATPFYICPPPFFRPKLPKQAGMP 186
P P + P P RPK P+ +P
Sbjct: 422 PALAPPALNPLPMPRPKRPRANVVP 496
>gb|BI308510.1|BI308510 EST529920 GPOD Medicago truncatula cDNA clone pGPOD-7G21 5' end,
mRNA sequence
Length = 521
Score = 88.1 bits (186), Expect(2) = 2e-032
Identities = 32/42 (76%), Positives = 38/42 (90%)
Frame = -1 / +2
Query: 443 RFRMLFETEDSGVRRYMGTITGIGDLDPLRWKNSHWRNLQVG 318
RFRMLFETE+S VRRYMGTIT I D+DP+RW NSHWR+++VG
Sbjct: 335 RFRMLFETEESSVRRYMGTITSISDMDPVRWPNSHWRSVRVG 460
Score = 72.5 bits (152), Expect(2) = 2e-032
Identities = 30/54 (55%), Positives = 37/54 (68%)
Frame = -1 / +2
Query: 626 LSSSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPRAXPSEFVIPLAKYNKALY 465
+ SS S M + AA HAAA + FT+F+NPRA PSEFVIPL+KY KA+Y
Sbjct: 146 MPSSVLSSDSMHIGLLAAAAHAAATNSCFTVFFNPRASPSEFVIPLSKYIKAVY 307
>gb|AW688927.1|AW688927 NF013C11ST1F1000 Developing stem Medicago truncatula cDNA clone
NF013C11ST 5', mRNA sequence
Length = 398
Score = 85.4 bits (180), Expect(2) = 2e-032
Identities = 35/55 (63%), Positives = 41/55 (74%)
Frame = -1 / +1
Query: 626 LSSSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPRAXPSEFVIPLAKYNKALYT 462
LSSS S M I AA HA+AN+ PFT+FYNPRA PSEFVIPLAKY +A+Y+
Sbjct: 112 LSSSVLSSDSMHIGILAAAAHASANNSPFTVFYNPRASPSEFVIPLAKYYRAVYS 276
Score = 75.3 bits (158), Expect(2) = 2e-032
Identities = 29/45 (64%), Positives = 33/45 (73%)
Frame = -1 / +1
Query: 482 YNKALYTQVSLGMRFRMLFETEDSGVRRYMGTITGIGDLDPLRWK 348
Y Q+S GMRFRM+FETEDSG RRYMGT G+ DLD +RWK
Sbjct: 259 YRAVYSHQISPGMRFRMMFETEDSGTRRYMGTXIGVSDLDSVRWK 393
>gb|AC151529.20| Medicago truncatula clone mth2-7n23, complete sequence
Length = 134908
Score = 59.7 bits (124), Expect(5) = 7e-032
Identities = 21/30 (70%), Positives = 26/30 (86%)
Frame = -1 / -2
Query: 401 RYMGTITGIGDLDPLRWKNSHWRNLQVGWD 312
RYMGTITGI DLD +RW+NSHWR+++V D
Sbjct: 48444 RYMGTITGISDLDAVRWQNSHWRSVKVSKD 48355
Score = 52.4 bits (108), Expect(5) = 7e-032
Identities = 19/25 (76%), Positives = 22/25 (88%)
Frame = -1 / -2
Query: 323 VGWDESTASERRTRVSIWEIEPVAT 249
VGWDESTA ER+ RVS+WEIEP+ T
Sbjct: 47583 VGWDESTAGERQPRVSLWEIEPLTT 47509
Score = 49.2 bits (101), Expect(5) = 7e-032
Identities = 19/27 (70%), Positives = 24/27 (88%)
Frame = -1 / -1
Query: 467 YTQVSLGMRFRMLFETEDSGVRRYMGT 387
+T++S+GMRFRMLFETE+S VRRY T
Sbjct: 48805 HTRISVGMRFRMLFETEESSVRRYF*T 48725
Score = 40.9 bits (83), Expect(5) = 7e-032
Identities = 16/25 (64%), Positives = 20/25 (80%)
Frame = -1 / -1
Query: 539 TIFYNPRAXPSEFVIPLAKYNKALY 465
T++ RA PSEFVIPL+KY KA+Y
Sbjct: 48880 TVYIMGRASPSEFVIPLSKYIKAVY 48806
Score = 40.0 bits (81), Expect(5) = 7e-032
Identities = 16/36 (44%), Positives = 21/36 (58%)
Frame = -1 / -1
Query: 626 LSSSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPR 519
+ SS S M + AA HA+A + FT+FYNPR
Sbjct: 49051 MPSSVLSSDSMHIGLLAAAAHASATNSCFTVFYNPR 48944
>gb|AC144478.20| Medicago truncatula clone mth2-20b20, complete sequence
Length = 144248
Score = 59.7 bits (124), Expect(5) = 3e-031
Identities = 27/48 (56%), Positives = 31/48 (64%)
Frame = -1 / +1
Query: 329 LQVGWDESTASERRTRVSIWEIEPVATPFYICPPPFFRPKLPKQAGMP 186
LQVGWDESTA ER+ RVS+WEIEP+ T P R K P G+P
Sbjct: 58795 LQVGWDESTAGERQPRVSLWEIEPLTTFPMYPSPFPLRLKRPWPPGLP 58938
Score = 56.1 bits (116), Expect(5) = 3e-031
Identities = 20/27 (74%), Positives = 24/27 (88%)
Frame = -1 / +3
Query: 401 RYMGTITGIGDLDPLRWKNSHWRNLQV 321
RYMGTITGI DLD +RW NSHWR+++V
Sbjct: 58557 RYMGTITGICDLDSVRWPNSHWRSVKV 58637
Score = 46.0 bits (94), Expect(5) = 3e-031
Identities = 18/23 (78%), Positives = 22/23 (95%)
Frame = -1 / +3
Query: 467 YTQVSLGMRFRMLFETEDSGVRR 399
+T+VS+GMRFRMLFETE+S VRR
Sbjct: 58332 HTRVSVGMRFRMLFETEESSVRR 58400
Score = 42.3 bits (86), Expect(5) = 3e-031
Identities = 18/36 (50%), Positives = 21/36 (58%)
Frame = -1 / +3
Query: 626 LSSSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPR 519
+ SS S M + AA HAAA + FTIFYNPR
Sbjct: 58080 MPSSVLSSDSMHLGLLAAAAHAAATNSRFTIFYNPR 58187
Score = 36.3 bits (73), Expect(5) = 3e-031
Identities = 15/18 (83%), Positives = 16/18 (88%)
Frame = -1 / +3
Query: 518 AXPSEFVIPLAKYNKALY 465
A PSEFVIPLAKY KA+Y
Sbjct: 58278 ACPSEFVIPLAKYVKAVY 58331
>gb|BG646172.1|BG646172 EST507791 KV3 Medicago truncatula cDNA clone pKV3-48N21 5' end,
mRNA sequence
Length = 802
Score = 135 bits (290), Expect = 5e-031
Identities = 61/129 (47%), Positives = 78/129 (60%)
Frame = -1 / +1
Query: 425 ETEDSGVRRYMGTITGIGDLDPLRWKNSHWRNLQVGWDESTASERRTRVSIWEIEPVATP 246
ETE+S VRRYMGTITGI DLD +RW NSHWR+++VGWDESTA ER+ RVS+WEIEP+ T
Sbjct: 1 ETEESSVRRYMGTITGISDLDSVRWPNSHWRSVKVGWDESTAGERQPRVSLWEIEPLTTF 180
Query: 245 FYICPPPFFRPKLPKQAGMPDDENEVESAFKRAMPWLADDFALKDVQNALFPGLSLVQWM 66
P R K P G+P + F P L A + +Q F G+ + WM
Sbjct: 181 PMYPSPFPLRLKRPWPPGLPSFHGLKDDDFGMNSPLLWLRDADRGLQTLNFQGMGVNPWM 360
Query: 65 AMQQNPQML 39
+ +P M+
Sbjct: 361 QARLDPSMM 387
>gb|AC127428.38| Medicago truncatula clone mth2-36c19, WORKING DRAFT SEQUENCE, 3 ordered
pieces
Length = 274828
Score = 59.7 bits (124), Expect(5) = 4e-030
Identities = 27/48 (56%), Positives = 31/48 (64%)
Frame = -1 / -3
Query: 329 LQVGWDESTASERRTRVSIWEIEPVATPFYICPPPFFRPKLPKQAGMP 186
LQVGWDESTA ER+ RVS+WEIEP+ T P R K P G+P
Sbjct: 223244 LQVGWDESTAGERQPRVSLWEIEPLTTFPMYPSPFPLRLKRPWPPGLP 223101
Score = 56.1 bits (116), Expect(5) = 4e-030
Identities = 20/27 (74%), Positives = 24/27 (88%)
Frame = -1 / -2
Query: 401 RYMGTITGIGDLDPLRWKNSHWRNLQV 321
RYMGTITGI DLD +RW NSHWR+++V
Sbjct: 223482 RYMGTITGICDLDSVRWPNSHWRSVKV 223402
Score = 46.0 bits (94), Expect(5) = 4e-030
Identities = 18/23 (78%), Positives = 22/23 (95%)
Frame = -1 / -2
Query: 467 YTQVSLGMRFRMLFETEDSGVRR 399
+T+VS+GMRFRMLFETE+S VRR
Sbjct: 223707 HTRVSVGMRFRMLFETEESSVRR 223639
Score = 42.3 bits (86), Expect(5) = 4e-030
Identities = 18/36 (50%), Positives = 21/36 (58%)
Frame = -1 / -2
Query: 626 LSSSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPR 519
+ SS S M + AA HAAA + FTIFYNPR
Sbjct: 223959 MPSSVLSSDSMHLGLLAAAAHAAATNSRFTIFYNPR 223852
Score = 36.3 bits (73), Expect(5) = 4e-030
Identities = 15/18 (83%), Positives = 16/18 (88%)
Frame = -1 / -2
Query: 518 AXPSEFVIPLAKYNKALY 465
A PSEFVIPLAKY KA+Y
Sbjct: 223761 ACPSEFVIPLAKYVKAVY 223708
>gb|AW689134.1|AW689134 NF015G05ST1F1000 Developing stem Medicago truncatula cDNA clone
NF015G05ST 5', mRNA sequence
Length = 623
Score = 120 bits (257), Expect(2) = 2e-027
Identities = 49/118 (41%), Positives = 68/118 (57%)
Frame = -1 / +3
Query: 620 SSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPRAXPSEFVIPLAKYNKALYTQVSLGMR 441
SS S M + A HA FT++Y PR P+EF++P +Y ++L ++GMR
Sbjct: 156 SSVISSHSMHLGVLATAWHAVLTGTMFTVYYKPRTSPAEFIVPYDQYMESLKNNYTIGMR 335
Query: 440 FRMLFETEDSGVRRYMGTITGIGDLDPLRWKNSHWRNLQVGWDESTASERRTRVSIWE 267
F+M FE E + +R+ GTI GI D D RW S WR L+V WDE++ R RVS W+
Sbjct: 336 FKMRFEGEXAPEQRFTGTIVGIEDSDSKRWPTSKWRCLKVRWDETSNIPRPERVSPWK 509
Score = 23.1 bits (44), Expect(2) = 2e-027
Identities = 10/22 (45%), Positives = 11/22 (50%)
Frame = -1 / +2
Query: 269 EIEPVATPFYICPPPFFRPKLP 204
EIEP P + P P PK P
Sbjct: 506 EIEPALAPPALNPXPMPXPKRP 571
>gb|CX534173.1|CX534173 s13dNF0TC01MJ002_322105 Methyl Jasmonate-Elicited Root Cell
Suspension Culture Medicago truncatula cDNA, mRNA
sequence
Length = 580
Score = 113 bits (241), Expect = 3e-024
Identities = 44/106 (41%), Positives = 63/106 (59%)
Frame = -1 / +2
Query: 620 SSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPRAXPSEFVIPLAKYNKALYTQVSLGMR 441
SS S M + A HA FT++Y PR P+EF++P +Y ++L ++GMR
Sbjct: 251 SSVISSHSMHLGVLATAWHAVLTGTMFTVYYKPRTSPAEFIVPYDQYMESLKNNYTIGMR 430
Query: 440 FRMLFETEDSGVRRYMGTITGIGDLDPLRWKNSHWRNLQVGWDEST 303
F+M FE E++ +R+ GTI GI D D RW S WR L+V WDE++
Sbjct: 431 FKMRFEGEEAPEQRFTGTIVGIEDSDSKRWPTSKWRCLKVRWDETS 568
>gb|BI308096.1|BI308096 EST529506 GPOD Medicago truncatula cDNA clone pGPOD-2I13 5' end,
mRNA sequence
Length = 844
Score = 109 bits (232), Expect = 5e-023
Identities = 40/94 (42%), Positives = 59/94 (62%)
Frame = -1 / +3
Query: 584 IXAAXXHAAANSXPFTIFYNPRAXPSEFVIPLAKYNKALYTQVSLGMRFRMLFETEDSGV 405
+ A HA FT++Y PR P+EF++P +Y ++L ++GMRF+M FE E++
Sbjct: 549 VLATAWHAVLTGTMFTVYYKPRTSPAEFIVPYDQYMESLKNNYTIGMRFKMRFEGEEASE 728
Query: 404 RRYMGTITGIGDLDPLRWKNSHWRNLQVGWDEST 303
+R+ GTI GI D D RW S WR L+V WDE++
Sbjct: 729 QRFTGTIVGIEDSDSKRWPTSKWRCLKVRWDETS 830
>gb|CX540580.1|CX540580 s13dNF0CC09GS070_464436 Germinating Seed Medicago truncatula cDNA,
mRNA sequence
Length = 533
Score = 103 bits (219), Expect = 3e-021
Identities = 44/100 (44%), Positives = 61/100 (61%)
Frame = -1 / +1
Query: 485 KYNKALYTQVSLGMRFRMLFETEDSGVRRYMGTITGIGDLDPLRWKNSHWRNLQVGWDES 306
+Y ++L ++GMRF+M FE E++ +R+ GTI GI D D RW S WR L+V WDE+
Sbjct: 4 QYMESLKNNYTIGMRFKMRFEGEEAPEQRFTGTIVGIEDSDSKRWPTSKWRCLKVRWDET 183
Query: 305 TASERRTRVSIWEIEPVATPFYICPPPFFRPKLPKQAGMP 186
+ R RVS W+IEP P + P P RPK P+ +P
Sbjct: 184 SNIPRPERVSPWKIEPALAPPALNPLPMPRPKRPRANVVP 303
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 617,477,536
Number of Sequences: 392609
Number of extensions: 8796504
Number of successful extensions: 57226
Number of sequences better than 1.0e-020: 25
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 19410690530
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)