TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCN28g01.yg.2.1
(513 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AC150782.12| Medicago truncatula clone mth2-116a21, WORK... 153 2e-046
gb|AC158736.12| Medicago truncatula clone mth2-165n2, WORKI... 153 2e-046
gb|AC152887.20| Medicago truncatula clone mth2-91j4, WORKIN... 86 2e-039
gb|AC157648.18| Medicago truncatula clone mth2-68d9, comple... 86 2e-039
gb|AC158209.13| Medicago truncatula clone mth2-154h5, compl... 80 4e-037
gb|AC152184.1| Medicago truncatula chromosome 7 BAC clone m... 89 4e-035
gb|AC122723.6| Medicago truncatula clone mth2-5i15, complet... 89 4e-035
gb|AC137603.16| Medicago truncatula clone mth2-14b10, compl... 89 6e-034
emb|CR340101.1| mte1-71J6RM1 BAC end, cultivar Jemalong A17... 86 2e-033
gb|CG932720.1|CG932720 MBENN88TF mth2 Medicago truncatula g... 74 8e-026
gb|CG974089.1|CG974089 MBEKK50TF mth2 Medicago truncatula g... 74 8e-026
>gb|AC150782.12| Medicago truncatula clone mth2-116a21, WORKING DRAFT SEQUENCE, 3
unordered pieces
Length = 118726
Score = 153 bits (329), Expect(2) = 2e-046
Identities = 61/97 (62%), Positives = 78/97 (80%)
Frame = -1 / +3
Query: 495 LRLLLTVKESAMDVPTNLDARRRISFFANSLFMSMPDAPKVRQMLPFSVLTPYYKEDVLF 316
L +LT ++S ++P NL+ARRRI+FF+NSLFM+MP AP+V +ML FSVLTPYY E+VL+
Sbjct: 19551 LHTILTSRDSMQNIPINLEARRRIAFFSNSLFMNMPHAPQVEKMLAFSVLTPYYNEEVLY 19730
Query: 315 SSQALAEQNEDGVSILFYLQKIYPDEWKNFLERVHCE 205
S + L +NEDGVS L+YLQ IY DEWKNFLER+ E
Sbjct: 19731 SKEQLRTENEDGVSTLYYLQTIYDDEWKNFLERMRRE 19841
Score = 98.7 bits (209), Expect(2) = 2e-021
Identities = 52/86 (60%), Positives = 58/86 (67%)
Frame = +2 / -1
Query: 224 RKFFHSSG*IFCK*KRMETPSSFCSASACEEKRTSSL**GVKTEKGNICRTFGASGIDIK 403
RKFFHSS *I CK* R+ETPSSF S TSSL**GV+T K +I T+GA GI IK
Sbjct: 19822 RKFFHSSS*IVCK*YRVETPSSFSVLSCSLLYNTSSL**GVRTLKASIFSTWGAWGIFIK 19643
Query: 404 RELAKKDILLLASRFVGTSIADSFTV 481
EL KK IL LASRF+G +S V
Sbjct: 19642 SELLKKAILRLASRFIGMFCIESLEV 19565
Score = 52.8 bits (109), Expect(2) = 2e-046
Identities = 21/24 (87%), Positives = 22/24 (91%)
Frame = -1 / +3
Query: 162 LRLWASYRGQTLTRTVRGMMYQER 91
LRLWASYRGQTL+RTVRGMMY R
Sbjct: 19887 LRLWASYRGQTLSRTVRGMMYYYR 19958
Score = 24.0 bits (46), Expect(2) = 2e-021
Identities = 11/17 (64%), Positives = 12/17 (70%)
Frame = +2 / -1
Query: 101 YIIPLTVLVKVCPRYDA 151
YIIPLTV V PR +A
Sbjct: 19948 YIIPLTVRDSVWPR*EA 19898
>gb|AC158736.12| Medicago truncatula clone mth2-165n2, WORKING DRAFT SEQUENCE
Length = 118519
Score = 153 bits (329), Expect(2) = 2e-046
Identities = 61/97 (62%), Positives = 78/97 (80%)
Frame = -1 / -1
Query: 495 LRLLLTVKESAMDVPTNLDARRRISFFANSLFMSMPDAPKVRQMLPFSVLTPYYKEDVLF 316
L +LT ++S ++P NL+ARRRI+FF+NSLFM+MP AP+V +ML FSVLTPYY E+VL+
Sbjct: 13246 LHTILTSRDSMQNIPINLEARRRIAFFSNSLFMNMPHAPQVEKMLAFSVLTPYYNEEVLY 13067
Query: 315 SSQALAEQNEDGVSILFYLQKIYPDEWKNFLERVHCE 205
S + L +NEDGVS L+YLQ IY DEWKNFLER+ E
Sbjct: 13066 SKEQLRTENEDGVSTLYYLQTIYDDEWKNFLERMRRE 12956
Score = 98.7 bits (209), Expect(2) = 2e-021
Identities = 52/86 (60%), Positives = 58/86 (67%)
Frame = +2 / +3
Query: 224 RKFFHSSG*IFCK*KRMETPSSFCSASACEEKRTSSL**GVKTEKGNICRTFGASGIDIK 403
RKFFHSS *I CK* R+ETPSSF S TSSL**GV+T K +I T+GA GI IK
Sbjct: 12975 RKFFHSSS*IVCK*YRVETPSSFSVLSCSLLYNTSSL**GVRTLKASIFSTWGAWGIFIK 13154
Query: 404 RELAKKDILLLASRFVGTSIADSFTV 481
EL KK IL LASRF+G +S V
Sbjct: 13155 SELLKKAILRLASRFIGMFCIESLEV 13232
Score = 52.8 bits (109), Expect(2) = 2e-046
Identities = 21/24 (87%), Positives = 22/24 (91%)
Frame = -1 / -1
Query: 162 LRLWASYRGQTLTRTVRGMMYQER 91
LRLWASYRGQTL+RTVRGMMY R
Sbjct: 12910 LRLWASYRGQTLSRTVRGMMYYYR 12839
Score = 24.0 bits (46), Expect(2) = 2e-021
Identities = 11/17 (64%), Positives = 12/17 (70%)
Frame = +2 / +3
Query: 101 YIIPLTVLVKVCPRYDA 151
YIIPLTV V PR +A
Sbjct: 12849 YIIPLTVRDSVWPR*EA 12899
>gb|AC152887.20| Medicago truncatula clone mth2-91j4, WORKING DRAFT SEQUENCE, 2 ordered
pieces
Length = 116277
Score = 86.3 bits (182), Expect(3) = 2e-039
Identities = 36/50 (72%), Positives = 44/50 (88%)
Frame = -1 / -3
Query: 495 LRLLLTVKESAMDVPTNLDARRRISFFANSLFMSMPDAPKVRQMLPFSVL 346
L LLLTVKESA++VP N++ARRRI+FFANSLFM+MP APKVR ML F ++
Sbjct: 100321 LHLLLTVKESAINVPQNIEARRRITFFANSLFMNMPKAPKVRDMLSFRLV 100172
Score = 76.7 bits (161), Expect(3) = 2e-039
Identities = 30/49 (61%), Positives = 39/49 (79%)
Frame = -1 / -3
Query: 357 FSVLTPYYKEDVLFSSQALAEQNEDGVSILFYLQKIYPDEWKNFLERVH 211
FSVLTPYYKE+V +S+ L ++NEDG+SILFYL KIYP E+ ++ R H
Sbjct: 100102 FSVLTPYYKENVQYSNDELKKENEDGISILFYLTKIYPGEYCFYMLRCH 99956
Score = 38.6 bits (78), Expect(3) = 2e-039
Identities = 15/21 (71%), Positives = 17/21 (80%)
Frame = -1 / -2
Query: 180 EHSEEQLRLWASYRGQTLTRT 118
E EE +R WASYRGQTL+RT
Sbjct: 99851 EDREEYVRQWASYRGQTLSRT 99789
>gb|AC157648.18| Medicago truncatula clone mth2-68d9, complete sequence
Length = 84361
Score = 86.3 bits (182), Expect(3) = 2e-039
Identities = 36/50 (72%), Positives = 44/50 (88%)
Frame = -1 / +1
Query: 495 LRLLLTVKESAMDVPTNLDARRRISFFANSLFMSMPDAPKVRQMLPFSVL 346
L LLLTVKESA++VP N++ARRRI+FFANSLFM+MP APKVR ML F ++
Sbjct: 73513 LHLLLTVKESAINVPQNIEARRRITFFANSLFMNMPKAPKVRDMLSFRLV 73662
Score = 76.7 bits (161), Expect(3) = 2e-039
Identities = 30/49 (61%), Positives = 39/49 (79%)
Frame = -1 / +1
Query: 357 FSVLTPYYKEDVLFSSQALAEQNEDGVSILFYLQKIYPDEWKNFLERVH 211
FSVLTPYYKE+V +S+ L ++NEDG+SILFYL KIYP E+ ++ R H
Sbjct: 73732 FSVLTPYYKENVQYSNDELKKENEDGISILFYLTKIYPGEYCFYMLRCH 73878
Score = 38.6 bits (78), Expect(3) = 2e-039
Identities = 15/21 (71%), Positives = 17/21 (80%)
Frame = -1 / +3
Query: 180 EHSEEQLRLWASYRGQTLTRT 118
E EE +R WASYRGQTL+RT
Sbjct: 73983 EDREEYVRQWASYRGQTLSRT 74045
>gb|AC158209.13| Medicago truncatula clone mth2-154h5, complete sequence
Length = 93864
Score = 79.9 bits (168), Expect(3) = 4e-037
Identities = 36/53 (67%), Positives = 41/53 (77%)
Frame = -1 / -3
Query: 513 FFFC*ALRLLLTVKESAMDVPTNLDARRRISFFANSLFMSMPDAPKVRQMLPF 355
FF L LLLTVKESA++VPTN + RRRI+FF NSLFM MP AP VR+ML F
Sbjct: 59923 FFQLRRLYLLLTVKESAIEVPTNSEVRRRIAFFTNSLFMDMPRAPCVRKMLSF 59765
Score = 65.2 bits (136), Expect(3) = 4e-037
Identities = 24/41 (58%), Positives = 33/41 (80%)
Frame = -1 / -1
Query: 366 MLPFSVLTPYYKEDVLFSSQALAEQNEDGVSILFYLQKIYP 244
+L FSVLTPYY E+ ++S + +NEDGVSI++YLQKI+P
Sbjct: 59256 LLLFSVLTPYYSEETVYSKNDIEVENEDGVSIIYYLQKIFP 59134
Score = 64.3 bits (134), Expect(3) = 4e-037
Identities = 24/45 (53%), Positives = 32/45 (71%)
Frame = -1 / -3
Query: 252 IYPDEWKNFLERVHCESEDQLHETEHSEEQLRLWASYRGQTLTRT 118
I PDEW NF+ER+ C+ + ++ E + + QLR WAS RGQTL RT
Sbjct: 59056 ILPDEWNNFMERLDCKKDSEIWEKDENILQLRHWASLRGQTLCRT 58922
>gb|AC152184.1| Medicago truncatula chromosome 7 BAC clone mte1-61c3, complete
sequence
Length = 103090
Score = 89.0 bits (188), Expect(3) = 4e-035
Identities = 39/48 (81%), Positives = 42/48 (87%)
Frame = -1 / +1
Query: 495 LRLLLTVKESAMDVPTNLDARRRISFFANSLFMSMPDAPKVRQMLPFS 352
L LLLT KESAMDVP+NL+ARRRISFF+NSLFM MP APKVR ML FS
Sbjct: 3811 LFLLLTTKESAMDVPSNLEARRRISFFSNSLFMDMPVAPKVRNMLSFS 3954
Score = 63.4 bits (132), Expect(3) = 4e-035
Identities = 26/36 (72%), Positives = 29/36 (80%)
Frame = -1 / +1
Query: 351 VLTPYYKEDVLFSSQALAEQNEDGVSILFYLQKIYP 244
+LTPYY E+VLFS L NEDGVSILFYLQKI+P
Sbjct: 4489 ILTPYYTEEVLFSLLDLDSPNEDGVSILFYLQKIFP 4596
Score = 50.6 bits (104), Expect(3) = 4e-035
Identities = 21/30 (70%), Positives = 25/30 (83%)
Frame = -1 / +2
Query: 207 ESEDQLHETEHSEEQLRLWASYRGQTLTRT 118
E E + +E+E EE+LRLWASYRGQTLTRT
Sbjct: 4739 EEELKGNESEELEEELRLWASYRGQTLTRT 4828
>gb|AC122723.6| Medicago truncatula clone mth2-5i15, complete sequence
Length = 105353
Score = 89.0 bits (188), Expect(3) = 4e-035
Identities = 39/48 (81%), Positives = 42/48 (87%)
Frame = -1 / +1
Query: 495 LRLLLTVKESAMDVPTNLDARRRISFFANSLFMSMPDAPKVRQMLPFS 352
L LLLT KESAMDVP+NL+ARRRISFF+NSLFM MP APKVR ML FS
Sbjct: 75208 LFLLLTTKESAMDVPSNLEARRRISFFSNSLFMDMPVAPKVRNMLSFS 75351
Score = 63.4 bits (132), Expect(3) = 4e-035
Identities = 26/36 (72%), Positives = 29/36 (80%)
Frame = -1 / +1
Query: 351 VLTPYYKEDVLFSSQALAEQNEDGVSILFYLQKIYP 244
+LTPYY E+VLFS L NEDGVSILFYLQKI+P
Sbjct: 75886 ILTPYYTEEVLFSLLDLDSPNEDGVSILFYLQKIFP 75993
Score = 50.6 bits (104), Expect(3) = 4e-035
Identities = 21/30 (70%), Positives = 25/30 (83%)
Frame = -1 / +2
Query: 207 ESEDQLHETEHSEEQLRLWASYRGQTLTRT 118
E E + +E+E EE+LRLWASYRGQTLTRT
Sbjct: 76136 EEELKGNESEELEEELRLWASYRGQTLTRT 76225
>gb|AC137603.16| Medicago truncatula clone mth2-14b10, complete sequence
Length = 109793
Score = 89.0 bits (188), Expect(4) = 6e-034
Identities = 38/48 (79%), Positives = 42/48 (87%)
Frame = -1 / -3
Query: 495 LRLLLTVKESAMDVPTNLDARRRISFFANSLFMSMPDAPKVRQMLPFS 352
L LLLT KESAMDVP+NL+A+RRISFF+NSLFM MP APKVR ML FS
Sbjct: 67125 LYLLLTTKESAMDVPSNLEAKRRISFFSNSLFMDMPTAPKVRNMLSFS 66982
Score = 73.9 bits (155), Expect(2) = 7e-026
Identities = 31/44 (70%), Positives = 35/44 (79%)
Frame = -1 / -1
Query: 486 LLTVKESAMDVPTNLDARRRISFFANSLFMSMPDAPKVRQMLPF 355
LLT+K+SA VP NL+ARRR+ FFANSLFM MP A VRQML F
Sbjct: 10898 LLTIKDSASSVPRNLEARRRLEFFANSLFMKMPHAKPVRQMLSF 10767
Score = 66.1 bits (138), Expect(4) = 6e-034
Identities = 27/36 (75%), Positives = 30/36 (83%)
Frame = -1 / -2
Query: 351 VLTPYYKEDVLFSSQALAEQNEDGVSILFYLQKIYP 244
VLTPYY E+VLFS + L NEDGVSILFYLQKI+P
Sbjct: 65593 VLTPYYTEEVLFSLRELESPNEDGVSILFYLQKIFP 65486
Score = 63.8 bits (133), Expect(2) = 7e-026
Identities = 25/37 (67%), Positives = 30/37 (81%)
Frame = -1 / -1
Query: 354 SVLTPYYKEDVLFSSQALAEQNEDGVSILFYLQKIYP 244
SV TPYY E VL+S L ++NEDG+SILFYLQKI+P
Sbjct: 10694 SVFTPYYSEIVLYSMDELLKKNEDGISILFYLQKIFP 10584
Score = 39.1 bits (79), Expect(4) = 6e-034
Identities = 16/35 (45%), Positives = 22/35 (62%)
Frame = -1 / -2
Query: 204 SEDQLHETEHSEEQLRLWASYRGQTLTRTVRGMMY 100
S+ L ++ +LR WASYRGQTL RT ++Y
Sbjct: 9007 SDTDLFDSASDILELRFWASYRGQTLARTGNVLIY 8903
Score = 29.0 bits (57), Expect(4) = 6e-034
Identities = 9/11 (81%), Positives = 10/11 (90%)
Frame = -1 / -2
Query: 246 PDEWKNFLERV 214
PDEWKNFL R+
Sbjct: 9058 PDEWKNFLARI 9026
>emb|CR340101.1| mte1-71J6RM1 BAC end, cultivar Jemalong A17 of Medicago truncatula,
genomic survey sequence
Length = 619
Score = 86.3 bits (182), Expect(2) = 2e-033
Identities = 36/50 (72%), Positives = 44/50 (88%)
Frame = -1 / +1
Query: 495 LRLLLTVKESAMDVPTNLDARRRISFFANSLFMSMPDAPKVRQMLPFSVL 346
L LLLTVKESA++VP N++ARRRI+FFANSLFM+MP APKVR ML F ++
Sbjct: 208 LHLLLTVKESAINVPQNIEARRRITFFANSLFMNMPKAPKVRDMLSFRLV 357
Score = 76.7 bits (161), Expect(2) = 2e-033
Identities = 30/49 (61%), Positives = 39/49 (79%)
Frame = -1 / +1
Query: 357 FSVLTPYYKEDVLFSSQALAEQNEDGVSILFYLQKIYPDEWKNFLERVH 211
FSVLTPYYKE+V +S+ L ++NEDG+SILFYL KIYP E+ ++ R H
Sbjct: 427 FSVLTPYYKENVQYSNDELKKENEDGISILFYLTKIYPGEYCFYMLRCH 573
>gb|CG932720.1|CG932720 MBENN88TF mth2 Medicago truncatula genomic clone 9O8, DNA sequence
Length = 904
Score = 73.9 bits (155), Expect(2) = 8e-026
Identities = 31/44 (70%), Positives = 35/44 (79%)
Frame = -1 / -1
Query: 486 LLTVKESAMDVPTNLDARRRISFFANSLFMSMPDAPKVRQMLPF 355
LLT+K+SA VP NL+ARRR+ FFANSLFM MP A VRQML F
Sbjct: 742 LLTIKDSASSVPRNLEARRRLEFFANSLFMKMPHAKPVRQMLSF 611
Score = 63.8 bits (133), Expect(2) = 8e-026
Identities = 25/37 (67%), Positives = 30/37 (81%)
Frame = -1 / -1
Query: 354 SVLTPYYKEDVLFSSQALAEQNEDGVSILFYLQKIYP 244
SV TPYY E VL+S L ++NEDG+SILFYLQKI+P
Sbjct: 538 SVFTPYYSEIVLYSMDELLKKNEDGISILFYLQKIFP 428
>gb|CG974089.1|CG974089 MBEKK50TF mth2 Medicago truncatula genomic clone 74J3, DNA sequence
Length = 886
Score = 73.9 bits (155), Expect(2) = 8e-026
Identities = 31/44 (70%), Positives = 35/44 (79%)
Frame = -1 / -3
Query: 486 LLTVKESAMDVPTNLDARRRISFFANSLFMSMPDAPKVRQMLPF 355
LLT+K+SA VP NL+ARRR+ FFANSLFM MP A VRQML F
Sbjct: 617 LLTIKDSASSVPRNLEARRRLEFFANSLFMKMPHAKPVRQMLSF 486
Score = 63.8 bits (133), Expect(2) = 8e-026
Identities = 25/37 (67%), Positives = 30/37 (81%)
Frame = -1 / -3
Query: 354 SVLTPYYKEDVLFSSQALAEQNEDGVSILFYLQKIYP 244
SV TPYY E VL+S L ++NEDG+SILFYLQKI+P
Sbjct: 413 SVFTPYYSEIVLYSMDELLKKNEDGISILFYLQKIFP 303
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 524,735,352
Number of Sequences: 392609
Number of extensions: 7579543
Number of successful extensions: 62075
Number of sequences better than 1.0e-020: 14
length of database: 147,244,331
effective HSP length: 53
effective length of database: 126,436,054
effective search space used: 14793018318
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)