TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCM12h10.yg.2.1
(339 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AC150782.12| Medicago truncatula clone mth2-116a21, WORK... 185 3e-046
gb|AC158736.12| Medicago truncatula clone mth2-165n2, WORKI... 185 3e-046
gb|AC152887.20| Medicago truncatula clone mth2-91j4, WORKIN... 142 2e-033
gb|AC157648.18| Medicago truncatula clone mth2-68d9, comple... 142 2e-033
gb|AC152184.1| Medicago truncatula chromosome 7 BAC clone m... 139 1e-032
gb|AC122723.6| Medicago truncatula clone mth2-5i15, complet... 139 1e-032
gb|AC158209.13| Medicago truncatula clone mth2-154h5, compl... 138 3e-032
gb|AC137603.16| Medicago truncatula clone mth2-14b10, compl... 133 7e-031
gb|BF641357.1|BF641357 NF061B11IN1F1092 Insect herbivory Me... 133 1e-030
gb|BG449853.1|BG449853 NF053H03IN1F1029 Insect herbivory Me... 117 5e-026
>gb|AC150782.12| Medicago truncatula clone mth2-116a21, WORKING DRAFT SEQUENCE, 3
unordered pieces
Length = 118726
Score = 185 bits (398), Expect = 3e-046
Identities = 74/111 (66%), Positives = 86/111 (77%)
Frame = -3 / +3
Query: 334 SSVXXTFSMGTKSXXXDXXILHGGAKYRATXRGFVVQHKSFAENYXLYARGHFIKAIELG 155
SSV TFSMGT+S ILHGGAKYRAT RGFVV+HKSFAE Y L++R HF+KAIELG
Sbjct: 21258 SSVFYTFSMGTRSHFFGRTILHGGAKYRATGRGFVVEHKSFAEIYRLFSRSHFVKAIELG 21437
Query: 154 IILTVYAAHSVIAKNTLVYIXXXXSSWFLVVSWIMAPFAFXXSGFDWLKTV 2
+IL +YA HS +A +T VYI +SWFLV SW++APF F SGFDWLKTV
Sbjct: 21438 LILVIYATHSPVATDTFVYIALTITSWFLVASWVVAPFVFNPSGFDWLKTV 21590
Score = 132 bits (282), Expect = 3e-030
Identities = 64/111 (57%), Positives = 70/111 (63%)
Frame = +3 / -1
Query: 3 TVFNQSKPXXXKANGAIIHDTTRNQLXXXXXM*TSVFLAITLCAAYTVNIIPSSIAFMKW 182
TVFNQSKP NGA HD T+NQL +*T+V +A LC A +I P+SIAF KW
Sbjct: 21589 TVFNQSKPEGLNTNGATTHDATKNQLVMVRAI*TNVSVATGLCVA*ITSISPNSIAFTKW 21410
Query: 183 PLAYSX*FSAKLLCCTTKPRXVARYFAPP*RIXXSXXWLLVPMENVXNTDE 335
S * SAKLLC TTKPR VARYFAPP RI L VPME V TDE
Sbjct: 21409 LREKSL*ISAKLLCSTTKPRPVARYFAPPCRIVRPKK*LRVPMEKV*KTDE 21257
>gb|AC158736.12| Medicago truncatula clone mth2-165n2, WORKING DRAFT SEQUENCE
Length = 118519
Score = 185 bits (398), Expect = 3e-046
Identities = 74/111 (66%), Positives = 86/111 (77%)
Frame = -3 / -1
Query: 334 SSVXXTFSMGTKSXXXDXXILHGGAKYRATXRGFVVQHKSFAENYXLYARGHFIKAIELG 155
SSV TFSMGT+S ILHGGAKYRAT RGFVV+HKSFAE Y L++R HF+KAIELG
Sbjct: 11539 SSVFYTFSMGTRSHFFGRTILHGGAKYRATGRGFVVEHKSFAEIYRLFSRSHFVKAIELG 11360
Query: 154 IILTVYAAHSVIAKNTLVYIXXXXSSWFLVVSWIMAPFAFXXSGFDWLKTV 2
+IL +YA HS +A +T VYI +SWFLV SW++APF F SGFDWLKTV
Sbjct: 11359 LILVIYATHSPVATDTFVYIALTITSWFLVASWVVAPFVFNPSGFDWLKTV 11207
Score = 132 bits (282), Expect = 3e-030
Identities = 64/111 (57%), Positives = 70/111 (63%)
Frame = +3 / +3
Query: 3 TVFNQSKPXXXKANGAIIHDTTRNQLXXXXXM*TSVFLAITLCAAYTVNIIPSSIAFMKW 182
TVFNQSKP NGA HD T+NQL +*T+V +A LC A +I P+SIAF KW
Sbjct: 11208 TVFNQSKPEGLNTNGATTHDATKNQLVMVRAI*TNVSVATGLCVA*ITSISPNSIAFTKW 11387
Query: 183 PLAYSX*FSAKLLCCTTKPRXVARYFAPP*RIXXSXXWLLVPMENVXNTDE 335
S * SAKLLC TTKPR VARYFAPP RI L VPME V TDE
Sbjct: 11388 LREKSL*ISAKLLCSTTKPRPVARYFAPPCRIVRPKK*LRVPMEKV*KTDE 11540
>gb|AC152887.20| Medicago truncatula clone mth2-91j4, WORKING DRAFT SEQUENCE, 2 ordered
pieces
Length = 116277
Score = 142 bits (304), Expect = 2e-033
Identities = 53/111 (47%), Positives = 73/111 (65%)
Frame = -3 / -1
Query: 334 SSVXXTFSMGTKSXXXDXXILHGGAKYRATXRGFVVQHKSFAENYXLYARGHFIKAIELG 155
+SV TF +GTK+ +LHGG+KYR T RGFVV H FA+NY +Y+R HF+K +E+
Sbjct: 97254 ASVFFTFQLGTKAHYYGRTLLHGGSKYRPTGRGFVVFHAKFADNYRMYSRSHFVKGLEIL 97075
Query: 154 IILTVYAAHSVIAKNTLVYIXXXXSSWFLVVSWIMAPFAFXXSGFDWLKTV 2
I+L +Y + +++ +Y S WFL +SW+ APF F SGFDW KTV
Sbjct: 97074 ILLIIYEVYGESYRSSTLYFFITISMWFLAISWLFAPFLFNPSGFDWQKTV 96922
>gb|AC157648.18| Medicago truncatula clone mth2-68d9, complete sequence
Length = 84361
Score = 142 bits (304), Expect = 2e-033
Identities = 53/111 (47%), Positives = 73/111 (65%)
Frame = -3 / +2
Query: 334 SSVXXTFSMGTKSXXXDXXILHGGAKYRATXRGFVVQHKSFAENYXLYARGHFIKAIELG 155
+SV TF +GTK+ +LHGG+KYR T RGFVV H FA+NY +Y+R HF+K +E+
Sbjct: 76580 ASVFFTFQLGTKAHYYGRTLLHGGSKYRPTGRGFVVFHAKFADNYRMYSRSHFVKGLEIL 76759
Query: 154 IILTVYAAHSVIAKNTLVYIXXXXSSWFLVVSWIMAPFAFXXSGFDWLKTV 2
I+L +Y + +++ +Y S WFL +SW+ APF F SGFDW KTV
Sbjct: 76760 ILLIIYEVYGESYRSSTLYFFITISMWFLAISWLFAPFLFNPSGFDWQKTV 76912
>gb|AC152184.1| Medicago truncatula chromosome 7 BAC clone mte1-61c3, complete
sequence
Length = 103090
Score = 139 bits (299), Expect = 1e-032
Identities = 56/109 (51%), Positives = 71/109 (65%)
Frame = -3 / +1
Query: 328 VXXTFSMGTKSXXXDXXILHGGAKYRATXRGFVVQHKSFAENYXLYARGHFIKAIELGII 149
V TFS+GTK+ +LHGGAKYR T RGFVV H FA+NY LY+R HF+K IEL ++
Sbjct: 8296 VFFTFSLGTKTHYFGRTLLHGGAKYRPTGRGFVVFHAKFADNYRLYSRSHFVKGIELLVL 8475
Query: 148 LTVYAAHSVIAKNTLVYIXXXXSSWFLVVSWIMAPFAFXXSGFDWLKTV 2
L VY S ++ + YI S WF+V +W+ APF F SGF+W K V
Sbjct: 8476 LVVYEIFSHSYRSAVAYILITVSMWFMVGTWLFAPFLFNPSGFEWQKIV 8622
>gb|AC122723.6| Medicago truncatula clone mth2-5i15, complete sequence
Length = 105353
Score = 139 bits (299), Expect = 1e-032
Identities = 56/109 (51%), Positives = 71/109 (65%)
Frame = -3 / +1
Query: 328 VXXTFSMGTKSXXXDXXILHGGAKYRATXRGFVVQHKSFAENYXLYARGHFIKAIELGII 149
V TFS+GTK+ +LHGGAKYR T RGFVV H FA+NY LY+R HF+K IEL ++
Sbjct: 79693 VFFTFSLGTKTHYFGRTLLHGGAKYRPTGRGFVVFHAKFADNYRLYSRSHFVKGIELLVL 79872
Query: 148 LTVYAAHSVIAKNTLVYIXXXXSSWFLVVSWIMAPFAFXXSGFDWLKTV 2
L VY S ++ + YI S WF+V +W+ APF F SGF+W K V
Sbjct: 79873 LVVYEIFSHSYRSAVAYILITVSMWFMVGTWLFAPFLFNPSGFEWQKIV 80019
>gb|AC158209.13| Medicago truncatula clone mth2-154h5, complete sequence
Length = 93864
Score = 138 bits (296), Expect = 3e-032
Identities = 53/107 (49%), Positives = 69/107 (64%)
Frame = -3 / -2
Query: 328 VXXTFSMGTKSXXXDXXILHGGAKYRATXRGFVVQHKSFAENYXLYARGHFIKAIELGII 149
V TFS+GTK +LHGGAKYRAT RGFVV+H+ FA+NY +Y+R HF+K IEL ++
Sbjct: 55922 VFFTFSLGTKLHYFGRTLLHGGAKYRATGRGFVVRHEKFADNYRMYSRSHFVKGIELALL 55743
Query: 148 LTVYAAHSVIAKNTLVYIXXXXSSWFLVVSWIMAPFAFXXSGFDWLK 8
L Y + ++ Y S WFLV SW+ +PF F SGF+W K
Sbjct: 55742 LICYMIYGAATPDSTAYALLSCSMWFLVGSWLFSPFLFNPSGFEWQK 55602
>gb|AC137603.16| Medicago truncatula clone mth2-14b10, complete sequence
Length = 109793
Score = 133 bits (286), Expect = 7e-031
Identities = 55/109 (50%), Positives = 69/109 (63%)
Frame = -3 / -1
Query: 328 VXXTFSMGTKSXXXDXXILHGGAKYRATXRGFVVQHKSFAENYXLYARGHFIKAIELGII 149
V TFS+GTK+ +LHGGAKYR T RGFVV H FA+NY LY+R HF+K IEL I+
Sbjct: 61766 VFFTFSLGTKTHYYGRTLLHGGAKYRPTGRGFVVFHAKFADNYRLYSRSHFVKGIELMIL 61587
Query: 148 LTVYAAHSVIAKNTLVYIXXXXSSWFLVVSWIMAPFAFXXSGFDWLKTV 2
L VY ++ L Y+ WF+V +W+ APF F SGF+W K V
Sbjct: 61586 LIVYQIFGNGYRSGLSYLLITTPMWFMVGTWLYAPFLFNPSGFEWQKIV 61440
>gb|BF641357.1|BF641357 NF061B11IN1F1092 Insect herbivory Medicago truncatula cDNA clone
NF061B11IN 5', mRNA sequence
Length = 660
Score = 133 bits (285), Expect = 1e-030
Identities = 56/84 (66%), Positives = 64/84 (76%)
Frame = -3 / +1
Query: 331 SVXXTFSMGTKSXXXDXXILHGGAKYRATXRGFVVQHKSFAENYXLYARGHFIKAIELGI 152
S+ TFS+GT++ ILHGGAKYRAT RGFVV+HKSFAENY LYAR HF+KAIELGI
Sbjct: 409 SLFFTFSLGTRTHFFGRTILHGGAKYRATGRGFVVEHKSFAENYRLYARSHFVKAIELGI 588
Query: 151 ILTVYAAHSVIAKNTLVYIXXXXS 80
IL VYA+HS + K T VYI S
Sbjct: 589 ILIVYASHSPLPKATFVYIAMTLS 660
Score = 104 bits (221), Expect = 7e-022
Identities = 49/79 (62%), Positives = 53/79 (67%)
Frame = +3 / -2
Query: 96 M*TSVFLAITLCAAYTVNIIPSSIAFMKWPLAYSX*FSAKLLCCTTKPRXVARYFAPP*R 275
M T V L L AYT+ IIPSS+A KW LAYS *FSAKLLC TTKPR VA Y APP*
Sbjct: 644 MYTKVALGKGL*EAYTIKIIPSSMALTKWLLAYSR*FSAKLLCSTTKPRPVALYLAPP*S 465
Query: 276 IXXSXXWLLVPMENVXNTD 332
I W+ VP ENV N+D
Sbjct: 464 IVRPKKWVRVPKENVKNSD 408
>gb|BG449853.1|BG449853 NF053H03IN1F1029 Insect herbivory Medicago truncatula cDNA clone
NF053H03IN 5', mRNA sequence
Length = 646
Score = 117 bits (251), Expect = 5e-026
Identities = 45/90 (50%), Positives = 58/90 (64%)
Frame = -3 / +3
Query: 277 ILHGGAKYRATXRGFVVQHKSFAENYXLYARGHFIKAIELGIILTVYAAHSVIAKNTLVY 98
+LHGGA+YR T RGFVV H FA+NY LY+R HF+K IEL I+L VY + + Y
Sbjct: 9 LLHGGAQYRGTGRGFVVFHAKFADNYRLYSRSHFVKGIELVILLVVYHIFGHAYRGVVAY 188
Query: 97 IXXXXSSWFLVVSWIMAPFAFXXSGFDWLK 8
I + WF+ +W+ APF F SGF+W K
Sbjct: 189 ILITITIWFMAGTWLFAPFLFNPSGFEWQK 278
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 298,293,463
Number of Sequences: 392609
Number of extensions: 3201893
Number of successful extensions: 89197
Number of sequences better than 1.0e-020: 14
length of database: 147,244,331
effective HSP length: 51
effective length of database: 127,221,272
effective search space used: 7760497592
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)