TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCH30c02.yg.2.1
(722 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
emb|CR954191.1| Medicago truncatula chromosome 5 clone mte1... 240 4e-065
emb|CR301510.1| mte1-2K10RM1 BAC end, cultivar Jemalong A17... 245 5e-064
gb|CG945567.1|CG945567 MBEMS48TR mth2 Medicago truncatula g... 172 1e-044
gb|CX526606.1|CX526606 s13dNF36F10AT091_513724 Aphid-Infect... 167 2e-043
gb|CG934467.1|CG934467 MBELJ71TR mth2 Medicago truncatula g... 160 3e-041
gb|CG942709.1|CG942709 MBEAH64TF mth2 Medicago truncatula g... 149 9e-038
emb|CR308299.1| mte1-29B10FM1 BAC end, cultivar Jemalong A1... 141 1e-032
gb|CG931676.1|CG931676 MBECQ89TR mth2 Medicago truncatula g... 125 1e-030
>emb|CR954191.1| Medicago truncatula chromosome 5 clone mte1-26e13, WORKING DRAFT
SEQUENCE
Length = 94044
Score = 240 bits (518), Expect(2) = 4e-065
Identities = 101/195 (51%), Positives = 134/195 (68%)
Frame = -2 / +1
Query: 670 ELXXXXXXXXIXXXXEQXAGXXEXSFSRWVXLXXXSKXXSMYIACSSYPFLDHDMFSVMA 491
EL I EQ + E + SRW+ L SK + +I LD+DMFS+M+
Sbjct: 30922 ELYHSICKNEIRTTPEQGSAFPEMTPSRWIYLIHKSKNTAPFIVSDCRAHLDYDMFSIMS 31101
Query: 490 GPTVAAVSVVFDNVEHEEVLTGCIDGFLSVAKLAAFYHLGDVLNDLVVALSKFTTLLTTS 311
GPTVAA+SVVFDN E EEV C+DGFL+VAK++A+YHL +L+DLVV+L KF T+L
Sbjct: 31102 GPTVAAISVVFDNAETEEVYQTCMDGFLAVAKVSAYYHLESILDDLVVSLCKFVTILDPL 31281
Query: 310 YIDDPVTAFGEDTKARMATEAVFTIATVYGDHIRSGWRNIIDCILRLHKIGLLPGPLTGD 131
++ + AFGEDTKARMATE VFTIA YGD+IR+GWRNI+DCIL+ HK+GLLP + D
Sbjct: 31282 SPEESILAFGEDTKARMATETVFTIANRYGDYIRTGWRNILDCILKFHKLGLLPAQMAND 31461
Query: 130 TADDQDSSSKSLPGK 86
A++ + S+++ GK
Sbjct: 31462 AAEESEPSTETGNGK 31506
Score = 71.6 bits (150), Expect(2) = 9e-027
Identities = 35/52 (67%), Positives = 39/52 (75%)
Frame = +3 / -2
Query: 150 GRSPILCNLKIQSIMFLHPLRM*SP*TVAIVKTASVAILALVSSPNAVTGSS 305
GR P L N K+QS +FLHP+RM*SP +AIVKT SVAILA VSSP A SS
Sbjct: 31442 GRRPNL*NFKMQSRIFLHPVRM*SPYLLAIVKTVSVAILAFVSSPKAKIDSS 31287
Score = 69.8 bits (146), Expect(2) = 9e-027
Identities = 39/80 (48%), Positives = 45/80 (56%)
Frame = +3 / -2
Query: 387 AASFATDRKPSMHPVRTSSCSTLSKTTETAATVGPAITENMS*SRNG*ELQAIYIDXLFD 566
A +FAT + PSMH TSS S LS TTE AATVGP I EN+S*SR + + + FD
Sbjct: 31205 ADTFATAKNPSMHVWYTSSVSALSNTTEMAATVGPDIMENIS*SRCALQSETMKGAVFFD 31026
Query: 567 XXXRXTQRLKXXSXXPAXCS 626
R QRL S CS
Sbjct: 31025 LCMR*IQRLGVISGKAEPCS 30966
Score = 29.9 bits (59), Expect(2) = 4e-065
Identities = 11/25 (44%), Positives = 19/25 (76%)
Frame = -2 / +1
Query: 76 SAPQVLPISTSRKTYGLMGRFSQLL 2
S+ Q+L ++T +++ G + RFSQLL
Sbjct: 31525 SSSQLLSVNTPKRSSGFISRFSQLL 31599
>emb|CR301510.1| mte1-2K10RM1 BAC end, cultivar Jemalong A17 of Medicago truncatula,
genomic survey sequence
Length = 555
Score = 245 bits (530), Expect = 5e-064
Identities = 99/170 (58%), Positives = 128/170 (75%)
Frame = -2 / +3
Query: 640 IXXXXEQXAGXXEXSFSRWVXLXXXSKXXSMYIACSSYPFLDHDMFSVMAGPTVAAVSVV 461
I EQ G E + SRW+ L SK + +I S +LDHDMF++M+GPT+AA+SVV
Sbjct: 42 IRTTPEQGVGFPEMTPSRWIDLMHKSKKTAPFIVSGSKAYLDHDMFAIMSGPTIAAISVV 221
Query: 460 FDNVEHEEVLTGCIDGFLSVAKLAAFYHLGDVLNDLVVALSKFTTLLTTSYIDDPVTAFG 281
FD+ EHE+V C+DGFL++AK++A +HL DVL+DLVV+L KFTTLL S +++PV AFG
Sbjct: 222 FDHAEHEDVYQTCMDGFLAIAKISACHHLEDVLDDLVVSLCKFTTLLNPSLVEEPVLAFG 401
Query: 280 EDTKARMATEAVFTIATVYGDHIRSGWRNIIDCILRLHKIGLLPGPLTGD 131
+D KARMAT VFTIA YGD+IR+GWRNI+DCILRLHK+GLLP + D
Sbjct: 402 DDMKARMATVTVFTIANRYGDYIRTGWRNILDCILRLHKLGLLPARVASD 551
Score = 133 bits (286), Expect = 2e-030
Identities = 74/159 (46%), Positives = 84/159 (52%)
Frame = +3 / -3
Query: 150 GRSPILCNLKIQSIMFLHPLRM*SP*TVAIVKTASVAILALVSSPNAVTGSSM*EVVNKV 329
GR P LCNLK+QS +FL P+RM*SP AIV T +VAILA +SSP A TGSS + V
Sbjct: 532 GRRPSLCNLKMQSRIFLQPVRM*SPYLFAIVNTVTVAILAFMSSPKARTGSSTNDGFRSV 353
Query: 330 VNLDXXXXXXXXXXXXXXNAASFATDRKPSMHPVRTSSCSTLSKTTETAATVGPAITENM 509
VNL A A + PS+H TSSCS S TTE AA VGP I NM
Sbjct: 352 VNLQSDTTRSSRTSSR*WQAEILAIAKNPSIHVWYTSSCSA*SNTTEMAAIVGPDIMANM 173
Query: 510 S*SRNG*ELQAIYIDXLFDXXXRXTQRLKXXSXXPAXCS 626
S*SR E + FD + RL S P CS
Sbjct: 172 S*SR*ALEPDTMKGAVFFDLCIKSIHRLGVISGNPTPCS 56
>gb|CG945567.1|CG945567 MBEMS48TR mth2 Medicago truncatula genomic clone 4H23, DNA sequence
Length = 880
Score = 172 bits (370), Expect(2) = 1e-044
Identities = 68/118 (57%), Positives = 92/118 (77%)
Frame = -2 / -2
Query: 424 CIDGFLSVAKLAAFYHLGDVLNDLVVALSKFTTLLTTSYIDDPVTAFGEDTKARMATEAV 245
CIDGFL++AK++A+YHL +VL+DL+V L KF T+L +++ V AFG+DTKARM TE V
Sbjct: 879 CIDGFLAIAKISAYYHLENVLDDLIVCLCKFFTILDPLSVEESVLAFGDDTKARMVTETV 700
Query: 244 FTIATVYGDHIRSGWRNIIDCILRLHKIGLLPGPLTGDTADDQDSSSKSLPGKLASSA 71
FTIA+ YGD+IR+GWRNI+DCI RLH +GLLP L D D+ + S+K+ GKL S++
Sbjct: 699 FTIASRYGDYIRTGWRNILDCIFRLHNLGLLPTHLASDAVDESEPSTKTGRGKLNSNS 526
Score = 29.0 bits (57), Expect(2) = 1e-044
Identities = 12/25 (48%), Positives = 18/25 (72%)
Frame = -2 / -2
Query: 76 SAPQVLPISTSRKTYGLMGRFSQLL 2
S+ + IST +++ GL+ RFSQLL
Sbjct: 522 SSTHLQSISTPKRSSGLLSRFSQLL 448
>gb|CX526606.1|CX526606 s13dNF36F10AT091_513724 Aphid-Infected Shoots Medicago truncatula
cDNA, mRNA sequence
Length = 613
Score = 167 bits (359), Expect(2) = 2e-043
Identities = 65/113 (57%), Positives = 89/113 (78%)
Frame = -2 / +1
Query: 424 CIDGFLSVAKLAAFYHLGDVLNDLVVALSKFTTLLTTSYIDDPVTAFGEDTKARMATEAV 245
C+DGFL+VAK++A+YHL +L+DLVV+L KF T+L ++ + AFGEDTKARMATE V
Sbjct: 16 CMDGFLAVAKVSAYYHLESILDDLVVSLCKFVTILDPLSPEESILAFGEDTKARMATETV 195
Query: 244 FTIATVYGDHIRSGWRNIIDCILRLHKIGLLPGPLTGDTADDQDSSSKSLPGK 86
FTIA YGD+IR+GWRNI+DCIL+ HK+GLLP + D A++ + S+++ GK
Sbjct: 196 FTIANRYGDYIRTGWRNILDCILKFHKLGLLPAQMANDAAEESEPSTETGNGK 354
Score = 29.9 bits (59), Expect(2) = 2e-043
Identities = 11/25 (44%), Positives = 19/25 (76%)
Frame = -2 / +1
Query: 76 SAPQVLPISTSRKTYGLMGRFSQLL 2
S+ Q+L ++T +++ G + RFSQLL
Sbjct: 373 SSSQLLSVNTPKRSSGFISRFSQLL 447
>gb|CG934467.1|CG934467 MBELJ71TR mth2 Medicago truncatula genomic clone 80K22, DNA
sequence
Length = 869
Score = 160 bits (345), Expect(2) = 3e-041
Identities = 64/114 (56%), Positives = 88/114 (77%)
Frame = -2 / -3
Query: 412 FLSVAKLAAFYHLGDVLNDLVVALSKFTTLLTTSYIDDPVTAFGEDTKARMATEAVFTIA 233
FL++AK++A+YHL +VL+DL+V L KF T+L +++ V AFG+DTKARM TE VFTIA
Sbjct: 867 FLAIAKISAYYHLKNVLDDLIVCLCKFFTILDPLSVEESVLAFGDDTKARMVTETVFTIA 688
Query: 232 TVYGDHIRSGWRNIIDCILRLHKIGLLPGPLTGDTADDQDSSSKSLPGKLASSA 71
+ YGD+IR+GWRNI+DCI RLH +GLLP L D D+ + S+K+ GKL S++
Sbjct: 687 SRYGDYIRTGWRNILDCIFRLHNLGLLPTHLASDAVDESEPSTKTGRGKLNSNS 526
Score = 29.0 bits (57), Expect(2) = 3e-041
Identities = 12/25 (48%), Positives = 18/25 (72%)
Frame = -2 / -3
Query: 76 SAPQVLPISTSRKTYGLMGRFSQLL 2
S+ + IST +++ GL+ RFSQLL
Sbjct: 522 SSTHLQSISTPKRSSGLLSRFSQLL 448
>gb|CG942709.1|CG942709 MBEAH64TF mth2 Medicago truncatula genomic clone 12L8, DNA sequence
Length = 845
Score = 149 bits (320), Expect(2) = 9e-038
Identities = 60/107 (56%), Positives = 81/107 (75%)
Frame = -2 / -2
Query: 391 AAFYHLGDVLNDLVVALSKFTTLLTTSYIDDPVTAFGEDTKARMATEAVFTIATVYGDHI 212
+A+YHL +VL+DL+V L KF T+L +++ V AFG+DTKARM TE VFTIA+ YGD+I
Sbjct: 844 SAYYHLENVLDDLIVCLCKFFTILDPLSVEESVLAFGDDTKARMVTETVFTIASRYGDYI 665
Query: 211 RSGWRNIIDCILRLHKIGLLPGPLTGDTADDQDSSSKSLPGKLASSA 71
R+GWRNI+DCI RLH +GLLP L D D+ + S+K+ GKL S++
Sbjct: 664 RTGWRNILDCIFRLHNLGLLPTHLASDAVDESEPSTKTGRGKLNSNS 524
Score = 29.0 bits (57), Expect(2) = 9e-038
Identities = 12/25 (48%), Positives = 18/25 (72%)
Frame = -2 / -2
Query: 76 SAPQVLPISTSRKTYGLMGRFSQLL 2
S+ + IST +++ GL+ RFSQLL
Sbjct: 520 SSTHLQSISTPKRSSGLLSRFSQLL 446
>emb|CR308299.1| mte1-29B10FM1 BAC end, cultivar Jemalong A17 of Medicago
truncatula, genomic survey sequence
Length = 729
Score = 141 bits (303), Expect = 1e-032
Identities = 59/120 (49%), Positives = 81/120 (67%)
Frame = -2 / +1
Query: 640 IXXXXEQXAGXXEXSFSRWVXLXXXSKXXSMYIACSSYPFLDHDMFSVMAGPTVAAVSVV 461
I E G + SRW+ L SK + YI S +LD+DMF +++GPT+AAVSVV
Sbjct: 370 IRTTPESGFGSPGMTPSRWISLIHKSKTTAPYIVSDSRAYLDYDMFVILSGPTIAAVSVV 549
Query: 460 FDNVEHEEVLTGCIDGFLSVAKLAAFYHLGDVLNDLVVALSKFTTLLTTSYIDDPVTAFG 281
FDN E+EEV CIDGFL++AK++A+YHL +VL+DL+V L KF T+L +++ V AFG
Sbjct: 550 FDNAENEEVYQTCIDGFLAIAKISAYYHLENVLDDLIVCLCKFFTILDPLSVEESVLAFG 729
>gb|CG931676.1|CG931676 MBECQ89TR mth2 Medicago truncatula genomic clone 28O9, DNA sequence
Length = 770
Score = 125 bits (268), Expect(2) = 1e-030
Identities = 51/91 (56%), Positives = 67/91 (73%)
Frame = -2 / -3
Query: 343 LSKFTTLLTTSYIDDPVTAFGEDTKARMATEAVFTIATVYGDHIRSGWRNIIDCILRLHK 164
L KF T+L +++ V AFG+DTKARM TE VFTIA+ YGD+IR+GWRNI+DCI RLH
Sbjct: 765 LCKFFTILDPLSVEESVLAFGDDTKARMVTETVFTIASRYGDYIRTGWRNILDCIFRLHN 586
Query: 163 IGLLPGPLTGDTADDQDSSSKSLPGKLASSA 71
+GLLP L D D+ + S+K+ GKL S++
Sbjct: 585 LGLLPTHLASDAVDESEPSTKTGRGKLNSNS 493
Score = 29.0 bits (57), Expect(2) = 1e-030
Identities = 12/25 (48%), Positives = 18/25 (72%)
Frame = -2 / -3
Query: 76 SAPQVLPISTSRKTYGLMGRFSQLL 2
S+ + IST +++ GL+ RFSQLL
Sbjct: 489 SSTHLQSISTPKRSSGLLSRFSQLL 415
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 568,156,791
Number of Sequences: 392609
Number of extensions: 7182341
Number of successful extensions: 39511
Number of sequences better than 1.0e-020: 9
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 23444080770
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)