TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCH2g03.yg.2.1
(537 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AC152184.1| Medicago truncatula chromosome 7 BAC clone m... 84 3e-046
gb|AC122723.6| Medicago truncatula clone mth2-5i15, complet... 84 3e-046
gb|AC137603.16| Medicago truncatula clone mth2-14b10, compl... 81 3e-042
gb|AW696467.2|AW696467 NF106E12ST1F1098 Developing stem Med... 90 6e-035
gb|CB893638.1|CB893638 EST646430 HOGA Medicago truncatula c... 70 1e-031
gb|AC158209.13| Medicago truncatula clone mth2-154h5, compl... 73 2e-031
gb|CG928497.1|CG928497 MBEJG66TF mth2 Medicago truncatula g... 71 5e-031
gb|AC150782.12| Medicago truncatula clone mth2-116a21, WORK... 89 7e-031
gb|AC158736.12| Medicago truncatula clone mth2-165n2, WORKI... 89 7e-031
gb|CB893388.1|CB893388 EST646180 HOGA Medicago truncatula c... 66 2e-029
gb|AJ500423.1|AJ500423 AJ500423 MTGIM Medicago truncatula c... 109 4e-023
>gb|AC152184.1| Medicago truncatula chromosome 7 BAC clone mte1-61c3, complete
sequence
Length = 103090
Score = 83.5 bits (176), Expect(5) = 3e-046
Identities = 36/43 (83%), Positives = 36/43 (83%)
Frame = +3 / +2
Query: 366 LDQVIYKIKLPGNAILXEGKPENQXHAIIFTRXXXXQTIDMNQ 494
LDQVIYKIKLPG AIL EGKPENQ HAIIFTR QTIDMNQ
Sbjct: 6632 LDQVIYKIKLPGPAILGEGKPENQNHAIIFTRGEGLQTIDMNQ 6760
Score = 80.8 bits (170), Expect(5) = 3e-046
Identities = 33/41 (80%), Positives = 35/41 (85%)
Frame = +3 / +1
Query: 105 LKTQCKAIADMKFTYVVSCQLYGIQKRSNEPCAHDILRLMT 227
L TQC+A+ADMKFTYVVSCQ YGI KRS P AHDILRLMT
Sbjct: 5233 LWTQCQAVADMKFTYVVSCQQYGIDKRSGSPRAHDILRLMT 5355
Score = 62.0 bits (129), Expect(5) = 3e-046
Identities = 27/34 (79%), Positives = 28/34 (82%)
Frame = +3 / +3
Query: 231 YPSLRVAYIDEVEAPSKDRIKKIEKVYYSVLVKA 332
YPSLRVAYIDEVE P K+ KKI KVYYS LVKA
Sbjct: 5475 YPSLRVAYIDEVEEPIKNSKKKINKVYYSCLVKA 5576
Score = 35.9 bits (72), Expect(5) = 3e-046
Identities = 13/14 (92%), Positives = 13/14 (92%)
Frame = +1 / +2
Query: 4 IEKLWSFRLFLIWP 45
IEKLWSFRL LIWP
Sbjct: 5000 IEKLWSFRLSLIWP 5041
Score = 26.7 bits (52), Expect(5) = 3e-046
Identities = 10/14 (71%), Positives = 12/14 (85%)
Frame = +3 / +1
Query: 495 EHYMEEXLKMXNLL 536
++YMEE LKM NLL
Sbjct: 6844 DNYMEEALKMRNLL 6885
>gb|AC122723.6| Medicago truncatula clone mth2-5i15, complete sequence
Length = 105353
Score = 83.5 bits (176), Expect(5) = 3e-046
Identities = 36/43 (83%), Positives = 36/43 (83%)
Frame = +3 / +2
Query: 366 LDQVIYKIKLPGNAILXEGKPENQXHAIIFTRXXXXQTIDMNQ 494
LDQVIYKIKLPG AIL EGKPENQ HAIIFTR QTIDMNQ
Sbjct: 78029 LDQVIYKIKLPGPAILGEGKPENQNHAIIFTRGEGLQTIDMNQ 78157
Score = 80.8 bits (170), Expect(5) = 3e-046
Identities = 33/41 (80%), Positives = 35/41 (85%)
Frame = +3 / +1
Query: 105 LKTQCKAIADMKFTYVVSCQLYGIQKRSNEPCAHDILRLMT 227
L TQC+A+ADMKFTYVVSCQ YGI KRS P AHDILRLMT
Sbjct: 76630 LWTQCQAVADMKFTYVVSCQQYGIDKRSGSPRAHDILRLMT 76752
Score = 62.0 bits (129), Expect(5) = 3e-046
Identities = 27/34 (79%), Positives = 28/34 (82%)
Frame = +3 / +3
Query: 231 YPSLRVAYIDEVEAPSKDRIKKIEKVYYSVLVKA 332
YPSLRVAYIDEVE P K+ KKI KVYYS LVKA
Sbjct: 76872 YPSLRVAYIDEVEEPIKNSKKKINKVYYSCLVKA 76973
Score = 35.9 bits (72), Expect(5) = 3e-046
Identities = 13/14 (92%), Positives = 13/14 (92%)
Frame = +1 / +2
Query: 4 IEKLWSFRLFLIWP 45
IEKLWSFRL LIWP
Sbjct: 76397 IEKLWSFRLSLIWP 76438
Score = 26.7 bits (52), Expect(5) = 3e-046
Identities = 10/14 (71%), Positives = 12/14 (85%)
Frame = +3 / +1
Query: 495 EHYMEEXLKMXNLL 536
++YMEE LKM NLL
Sbjct: 78241 DNYMEEALKMRNLL 78282
>gb|AC137603.16| Medicago truncatula clone mth2-14b10, complete sequence
Length = 109793
Score = 81.3 bits (171), Expect(4) = 3e-042
Identities = 36/54 (66%), Positives = 40/54 (74%)
Frame = +3 / -2
Query: 363 SLDQVIYKIKLPGNAILXEGKPENQXHAIIFTRXXXXQTIDMNQEHYMEEXLKM 524
+ +QVIYKIKLPG AIL EGKPENQ HAI+FTR QTIDMNQ Y E K+
Sbjct: 63562 NFNQVIYKIKLPGPAILGEGKPENQNHAIMFTRGEGLQTIDMNQV*YFYEKHKI 63401
Score = 70.7 bits (148), Expect(3) = 3e-021
Identities = 29/39 (74%), Positives = 30/39 (76%)
Frame = +3 / -2
Query: 378 IYKIKLPGNAILXEGKPENQXHAIIFTRXXXXQTIDMNQ 494
IY +KLPGN L EGKPENQ HAIIFTR QTIDMNQ
Sbjct: 6859 IYSVKLPGNPKLGEGKPENQNHAIIFTRGNAVQTIDMNQ 6743
Score = 68.9 bits (144), Expect(4) = 3e-042
Identities = 29/40 (72%), Positives = 32/40 (80%)
Frame = +3 / -3
Query: 105 LKTQCKAIADMKFTYVVSCQLYGIQKRSNEPCAHDILRLM 224
L TQC+A+ADMKF+YVVSCQ YGI KRS A DILRLM
Sbjct: 64866 LWTQCQAVADMKFSYVVSCQQYGIDKRSGAARAQDILRLM 64747
Score = 66.6 bits (139), Expect(4) = 3e-042
Identities = 28/34 (82%), Positives = 30/34 (88%)
Frame = +3 / -2
Query: 231 YPSLRVAYIDEVEAPSKDRIKKIEKVYYSVLVKA 332
YPSLRVAYIDEVE PSK+R K+I KVYYS LVKA
Sbjct: 64657 YPSLRVAYIDEVEEPSKERPKRISKVYYSCLVKA 64556
Score = 52.8 bits (109), Expect(3) = 3e-021
Identities = 22/40 (55%), Positives = 28/40 (70%)
Frame = +3 / -1
Query: 105 LKTQCKAIADMKFTYVVSCQLYGIQKRSNEPCAHDILRLM 224
L + +A AD+KFTYVV+CQ+YG QK +P A DI LM
Sbjct: 8057 LSPEARAQADLKFTYVVTCQIYGKQKEEQKPEAVDIALLM 7938
Score = 35.4 bits (71), Expect(4) = 3e-042
Identities = 14/17 (82%), Positives = 16/17 (94%)
Frame = +3 / -1
Query: 3 YRKALELQAFLDMANND 53
YRKALELQAFLDMA ++
Sbjct: 65123 YRKALELQAFLDMAKDE 65073
Score = 32.7 bits (65), Expect(3) = 3e-021
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = +1 / -2
Query: 1 TIEKLWSFRLFLIWPTMMI 57
T EK WS RLF IWP M +
Sbjct: 65125 TTEKPWSSRLFSIWPKMKV 65069
>gb|AW696467.2|AW696467 NF106E12ST1F1098 Developing stem Medicago truncatula cDNA clone
NF106E12ST 5', mRNA sequence
Length = 511
Score = 90.4 bits (191), Expect(2) = 6e-035
Identities = 38/51 (74%), Positives = 41/51 (80%)
Frame = +3 / +1
Query: 384 KIKLPGNAILXEGKPENQXHAIIFTRXXXXQTIDMNQEHYMEEXLKMXNLL 536
+IKLPG AIL EGKPENQ HAIIFTR QTIDMNQ++YMEE KM NLL
Sbjct: 178 RIKLPGPAILGEGKPENQNHAIIFTRGEGLQTIDMNQDNYMEEAFKMRNLL 330
Score = 78.0 bits (164), Expect(2) = 6e-035
Identities = 32/43 (74%), Positives = 38/43 (88%)
Frame = +3 / +3
Query: 210 ILRLMTEYPSLRVAYIDEVEAPSKDRIKKIEKVYYSVLVKASV 338
IL+LMT+YPSLRVAYIDEVE PSKD +KI+KVYYS L KA++
Sbjct: 3 ILKLMTKYPSLRVAYIDEVEEPSKDSSRKIDKVYYSALTKAAL 131
>gb|CB893638.1|CB893638 EST646430 HOGA Medicago truncatula cDNA clone HOGA-28F5, mRNA
sequence
Length = 841
Score = 70.3 bits (147), Expect(5) = 1e-031
Identities = 28/42 (66%), Positives = 32/42 (76%)
Frame = +3 / +3
Query: 411 LXEGKPENQXHAIIFTRXXXXQTIDMNQEHYMEEXLKMXNLL 536
+ EGKPENQ HAIIFTR QTIDMNQ++Y EE KM N+L
Sbjct: 522 IGEGKPENQNHAIIFTRGEALQTIDMNQDNYYEEAFKMRNVL 647
Score = 52.4 bits (108), Expect(5) = 1e-031
Identities = 20/25 (80%), Positives = 23/25 (92%)
Frame = +3 / +3
Query: 114 QCKAIADMKFTYVVSCQLYGIQKRS 188
Q KA+AD+KFTYVVSCQLYG QK+S
Sbjct: 249 QAKALADLKFTYVVSCQLYGSQKKS 323
Score = 35.4 bits (71), Expect(5) = 1e-031
Identities = 14/24 (58%), Positives = 18/24 (75%)
Frame = +3 / +3
Query: 198 CAHDILRLMTEYPSLRVAYIDEVE 269
C ++IL LM + +LRVAYIDE E
Sbjct: 345 CYNNILNLMVTHSALRVAYIDETE 416
Score = 28.1 bits (55), Expect(5) = 1e-031
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = +3 / +3
Query: 357 GQSLDQVIYKIKLPG 401
G+ DQ IY+IKLPG
Sbjct: 465 GEKYDQEIYRIKLPG 509
Score = 23.5 bits (45), Expect(5) = 1e-031
Identities = 9/10 (90%), Positives = 10/10 (100%)
Frame = +3 / +3
Query: 300 EKVYYSVLVK 329
+KVYYSVLVK
Sbjct: 432 KKVYYSVLVK 461
>gb|AC158209.13| Medicago truncatula clone mth2-154h5, complete sequence
Length = 93864
Score = 73.5 bits (154), Expect(4) = 2e-031
Identities = 31/47 (65%), Positives = 35/47 (74%)
Frame = +3 / -1
Query: 378 IYKIKLPGNAILXEGKPENQXHAIIFTRXXXXQTIDMNQEHYMEEXL 518
I++IKLPG A L EGKPENQ HAIIFTR QTIDMNQ Y++ L
Sbjct: 58008 IFRIKLPGPAKLGEGKPENQNHAIIFTRGEALQTIDMNQVKYIKRSL 57868
Score = 57.9 bits (120), Expect(4) = 2e-031
Identities = 25/43 (58%), Positives = 30/43 (69%)
Frame = +3 / -1
Query: 105 LKTQCKAIADMKFTYVVSCQLYGIQKRSNEPCAHDILRLMTEY 233
L +A+ADMKFTYV +CQ YG QKRS + A DIL LM +Y
Sbjct: 58443 LYANLEAMADMKFTYVATCQNYGNQKRSGDRRATDILNLMVKY 58315
Score = 33.1 bits (66), Expect(4) = 2e-031
Identities = 13/22 (59%), Positives = 17/22 (77%)
Frame = +3 / -1
Query: 234 PSLRVAYIDEVEAPSKDRIKKI 299
PSLRVAYIDEVE +++K+
Sbjct: 58200 PSLRVAYIDEVEEREGGQVQKV 58135
Score = 27.2 bits (53), Expect(4) = 2e-031
Identities = 9/13 (69%), Positives = 13/13 (100%)
Frame = +3 / -1
Query: 294 KIEKVYYSVLVKA 332
+++KVYYSVL+KA
Sbjct: 58149 QVQKVYYSVLIKA 58111
>gb|CG928497.1|CG928497 MBEJG66TF mth2 Medicago truncatula genomic clone 67L11, DNA
sequence
Length = 935
Score = 70.7 bits (148), Expect(4) = 5e-031
Identities = 30/39 (76%), Positives = 31/39 (79%)
Frame = +3 / -2
Query: 378 IYKIKLPGNAILXEGKPENQXHAIIFTRXXXXQTIDMNQ 494
IY+IKLPG A L EGKPENQ HAIIFTR QTIDMNQ
Sbjct: 247 IYRIKLPGPAKLGEGKPENQNHAIIFTRGEALQTIDMNQ 131
Score = 58.3 bits (121), Expect(4) = 5e-031
Identities = 24/43 (55%), Positives = 30/43 (69%)
Frame = +3 / -2
Query: 105 LKTQCKAIADMKFTYVVSCQLYGIQKRSNEPCAHDILRLMTEY 233
L +A+ADMKFTY+ +CQ YG QKRS + A DIL LM +Y
Sbjct: 670 LYASLEAVADMKFTYIATCQNYGNQKRSGDRHATDILNLMVKY 542
Score = 32.2 bits (64), Expect(4) = 5e-031
Identities = 12/22 (54%), Positives = 17/22 (77%)
Frame = +3 / -1
Query: 234 PSLRVAYIDEVEAPSKDRIKKI 299
PSLRVAYIDE+E +++K+
Sbjct: 440 PSLRVAYIDELEEREGGKVQKV 375
Score = 29.5 bits (58), Expect(4) = 5e-031
Identities = 11/13 (84%), Positives = 13/13 (100%)
Frame = +3 / -1
Query: 294 KIEKVYYSVLVKA 332
K++KVYYSVLVKA
Sbjct: 389 KVQKVYYSVLVKA 351
>gb|AC150782.12| Medicago truncatula clone mth2-116a21, WORKING DRAFT SEQUENCE, 3
unordered pieces
Length = 118726
Score = 89.0 bits (188), Expect(2) = 7e-031
Identities = 36/53 (67%), Positives = 41/53 (77%)
Frame = +3 / +3
Query: 378 IYKIKLPGNAILXEGKPENQXHAIIFTRXXXXQTIDMNQEHYMEEXLKMXNLL 536
+Y++KLPG L EGKPENQ HAIIFTR QTIDMNQ++Y EE LKM NLL
Sbjct: 20361 VYRVKLPGPLKLGEGKPENQNHAIIFTRGDALQTIDMNQDNYFEEALKMRNLL 20519
Score = 65.7 bits (137), Expect(2) = 7e-031
Identities = 26/43 (60%), Positives = 32/43 (74%)
Frame = +3 / +3
Query: 135 MKFTYVVSCQLYGIQKRSNEPCAHDILRLMTEYPSLRVAYIDE 263
MKFTYVV+CQ+YG QK +P A +IL LM +LRVAY+DE
Sbjct: 20154 MKFTYVVACQIYGTQKEKKDPHAEEILYLMKNNEALRVAYVDE 20282
>gb|AC158736.12| Medicago truncatula clone mth2-165n2, WORKING DRAFT SEQUENCE
Length = 118519
Score = 89.0 bits (188), Expect(2) = 7e-031
Identities = 36/53 (67%), Positives = 41/53 (77%)
Frame = +3 / -1
Query: 378 IYKIKLPGNAILXEGKPENQXHAIIFTRXXXXQTIDMNQEHYMEEXLKMXNLL 536
+Y++KLPG L EGKPENQ HAIIFTR QTIDMNQ++Y EE LKM NLL
Sbjct: 12436 VYRVKLPGPLKLGEGKPENQNHAIIFTRGDALQTIDMNQDNYFEEALKMRNLL 12278
Score = 65.7 bits (137), Expect(2) = 7e-031
Identities = 26/43 (60%), Positives = 32/43 (74%)
Frame = +3 / -1
Query: 135 MKFTYVVSCQLYGIQKRSNEPCAHDILRLMTEYPSLRVAYIDE 263
MKFTYVV+CQ+YG QK +P A +IL LM +LRVAY+DE
Sbjct: 12643 MKFTYVVACQIYGTQKEKKDPHAEEILYLMKNNEALRVAYVDE 12515
>gb|CB893388.1|CB893388 EST646180 HOGA Medicago truncatula cDNA clone HOGA-27P9, mRNA
sequence
Length = 512
Score = 66.1 bits (138), Expect(5) = 2e-029
Identities = 27/42 (64%), Positives = 30/42 (71%)
Frame = +3 / +1
Query: 411 LXEGKPENQXHAIIFTRXXXXQTIDMNQEHYMEEXLKMXNLL 536
+ EGKPENQ HAIIFTR Q IDMNQ +Y EE KM N+L
Sbjct: 277 IGEGKPENQNHAIIFTRGEALQNIDMNQGNYYEEAFKMRNVL 402
Score = 46.9 bits (96), Expect(5) = 2e-029
Identities = 19/25 (76%), Positives = 22/25 (88%)
Frame = +3 / +1
Query: 114 QCKAIADMKFTYVVSCQLYGIQKRS 188
Q KA+AD+KFTYVVS QLYG QK+S
Sbjct: 4 QAKALADLKFTYVVSGQLYGSQKKS 78
Score = 35.4 bits (71), Expect(5) = 2e-029
Identities = 14/24 (58%), Positives = 18/24 (75%)
Frame = +3 / +1
Query: 198 CAHDILRLMTEYPSLRVAYIDEVE 269
C ++IL LM + +LRVAYIDE E
Sbjct: 100 CYNNILNLMVTHSALRVAYIDETE 171
Score = 28.1 bits (55), Expect(5) = 2e-029
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = +3 / +1
Query: 357 GQSLDQVIYKIKLPG 401
G+ DQ IY+IKLPG
Sbjct: 220 GEKYDQEIYRIKLPG 264
Score = 23.5 bits (45), Expect(5) = 2e-029
Identities = 9/10 (90%), Positives = 10/10 (100%)
Frame = +3 / +1
Query: 300 EKVYYSVLVK 329
+KVYYSVLVK
Sbjct: 187 KKVYYSVLVK 216
>gb|AJ500423.1|AJ500423 AJ500423 MTGIM Medicago truncatula cDNA clone mtgmacc120016c03,
mRNA sequence
Length = 581
Score = 109 bits (232), Expect = 4e-023
Identities = 47/63 (74%), Positives = 51/63 (80%)
Frame = +3 / -2
Query: 348 NEPGQSLDQVIYKIKLPGNAILXEGKPENQXHAIIFTRXXXXQTIDMNQEHYMEEXLKMX 527
+E QSLDQVIY+IKLPG AIL EGKPENQ HAIIFTR QTIDMNQ++YMEE KM
Sbjct: 511 SEAVQSLDQVIYRIKLPGPAILGEGKPENQNHAIIFTRGEGLQTIDMNQDNYMEEAFKMR 332
Query: 528 NLL 536
NLL
Sbjct: 331 NLL 323
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 449,051,195
Number of Sequences: 392609
Number of extensions: 5466684
Number of successful extensions: 29853
Number of sequences better than 1.0e-020: 13
length of database: 147,244,331
effective HSP length: 53
effective length of database: 126,436,054
effective search space used: 15804506750
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)