TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCF7a10.yg.2.1
(523 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AW697303.1|AW697303 NF115B10ST1F1080 Developing stem Med... 193 8e-061
gb|DW018960.1|DW018960 EST1227921 MTY Medicago truncatula c... 189 3e-059
gb|BI308597.1|BI308597 EST530007 GPOD Medicago truncatula c... 187 2e-058
gb|AW691884.1|AW691884 NF045B08ST1F1000 Developing stem Med... 192 2e-055
gb|AW774471.1|AW774471 EST333622 KV3 Medicago truncatula cD... 164 1e-051
gb|BI272466.1|BI272466 NF021H08FL1F1075 Developing flower M... 137 8e-049
gb|AW574031.1|AW574031 EST316622 GVN Medicago truncatula cD... 189 2e-047
gb|AW686486.2|AW686486 NF041H10NR1F1000 Nodulated root Medi... 186 3e-047
gb|AC161863.20| Medicago truncatula clone mth2-175f4, compl... 82 3e-046
gb|BF646657.1|BF646657 NF076H12EC1F1103 Elicited cell cultu... 181 9e-045
gb|BE204599.1|BE204599 EST397275 KV0 Medicago truncatula cD... 171 5e-042
gb|BG582283.1|BG582283 EST484024 GVN Medicago truncatula cD... 116 2e-037
gb|BE321417.1|BE321417 NF024H12IN1F1096 Insect herbivory Me... 136 2e-036
gb|BE942980.1|BE942980 EST422559 MGHG Medicago truncatula c... 108 4e-035
gb|AW776261.1|AW776261 EST335326 DSIL Medicago truncatula c... 142 5e-033
emb|CR486384.1| mth2-155D12FM2 BAC end, cultivar Jemalong A... 83 8e-033
gb|BE321428.1|BE321428 NF025H12IN1F1096 Insect herbivory Me... 73 9e-029
gb|CX527137.1|CX527137 s13dNF40D04AT042_514786 Aphid-Infect... 82 5e-027
gb|DW016466.1|DW016466 EST1225427 MTY Medicago truncatula c... 102 6e-027
gb|BI271915.1|BI271915 NF016C03FL1F1021 Developing flower M... 60 3e-025
gb|CX527706.1|CX527706 s13dNF38E01AT006_515924 Aphid-Infect... 113 2e-024
gb|BF650120.1|BF650120 NF088C04EC1F1024 Elicited cell cultu... 109 3e-023
>gb|AW697303.1|AW697303 NF115B10ST1F1080 Developing stem Medicago truncatula cDNA clone
NF115B10ST 5', mRNA sequence
Length = 653
Score = 193 bits (416), Expect(2) = 8e-061
Identities = 81/113 (71%), Positives = 94/113 (83%)
Frame = +3 / +1
Query: 6 DARRKPRNVGKIIAALVLTTLCIFALKQSPGFGGNSVFSRHEPGVTHVLVTGGAGYIGSH 185
D ++K VGKI A LT+LCI +K+SP S F+ HEPGVTHVLVTGGAGYIGSH
Sbjct: 13 DPKKKGNFVGKIFLAAALTSLCIIMIKRSPSLKSPSPFAFHEPGVTHVLVTGGAGYIGSH 192
Query: 186 AALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFPQPGRLQFIFADLGDQKSVS 344
AA RLL+D+YRVTIVDNLSRGN+GAV+VLQ LFP+PGRLQFI+ADLGD+KSVS
Sbjct: 193 AAFRLLKDNYRVTIVDNLSRGNLGAVRVLQDLFPEPGRLQFIYADLGDKKSVS 351
Score = 144 bits (309), Expect(2) = 1e-043
Identities = 71/113 (62%), Positives = 76/113 (67%)
Frame = -1 / -1
Query: 343 DTDFWSPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLSTMVTR*LSCKSLKAACEPI*PA 164
DTDF SP+SA +N SLPG GN+ RT TAP FP DRLSTMVTR*LS + KAACEPI*PA
Sbjct: 350 DTDFLSPKSAYIN*SLPGSGNRS*RTLTAPKFPRDRLSTMVTR*LSFRRRKAACEPI*PA 171
Query: 163 PPVTKTWVTPGSWRENTLLPPKPGDCFRANIQSVVRTRAAMIFPTLRGFRLAS 5
PPVTKT VTPGSW+ N L K GD I S V A IFPT F S
Sbjct: 170 PPVTKT*VTPGSWKANGLGDFKDGDRLIMIIHSDVSAAARKIFPTKLPFFFGS 12
Score = 61.6 bits (128), Expect(2) = 8e-061
Identities = 26/34 (76%), Positives = 28/34 (82%)
Frame = +1 / +2
Query: 421 LTASQVNKIFAENAFDAVMHFAAXAYVGESTLEP 522
LT VNKIF EN FDAVMHFAA AYVGEST++P
Sbjct: 437 LTGY*VNKIFLENKFDAVMHFAAVAYVGESTVDP 538
Score = 53.3 bits (110), Expect(2) = 1e-043
Identities = 24/29 (82%), Positives = 24/29 (82%)
Frame = -3 / -3
Query: 521 GSNVLSPT*AXAAKCITASNAFSANILLT 435
GS VLSPT A AAKCITASN FS NILLT
Sbjct: 537 GSTVLSPTYATAAKCITASNLFSKNILLT 451
>gb|DW018960.1|DW018960 EST1227921 MTY Medicago truncatula cDNA clone MTYBD07, mRNA
sequence
Length = 741
Score = 189 bits (408), Expect(2) = 3e-059
Identities = 80/112 (71%), Positives = 90/112 (80%)
Frame = +3 / +3
Query: 6 DARRKPRNVGKIIAALVLTTLCIFALKQSPGFGGNSVFSRHEPGVTHVLVTGGAGYIGSH 185
D ++K +GK+ LT LCI +K+SP F S FS HEPGVTHVLVTGGAGYIGSH
Sbjct: 312 DPKKKGNLLGKVFLVAALTALCILVIKRSPSFTTPSPFSVHEPGVTHVLVTGGAGYIGSH 491
Query: 186 AALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFPQPGRLQFIFADLGDQKSV 341
A LRLL++SYRVTIVDNLSRGN+GAV+VLQ LFPQPGRLQFI ADLGD KSV
Sbjct: 492 ATLRLLKESYRVTIVDNLSRGNLGAVRVLQSLFPQPGRLQFIHADLGDAKSV 647
Score = 138 bits (295), Expect(2) = 1e-040
Identities = 64/103 (62%), Positives = 70/103 (67%)
Frame = -1 / -3
Query: 340 TDFWSPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLSTMVTR*LSCKSLKAACEPI*PAP 161
TDF SP+SA +N SLPGCGNK RT TAP FP DRLSTMVTR* S +S K AC+PI*PAP
Sbjct: 646 TDFASPKSACIN*SLPGCGNKL*RTLTAPKFPRDRLSTMVTR*DSFRSRKVACDPI*PAP 467
Query: 160 PVTKTWVTPGSWRENTLLPPKPGDCFRANIQSVVRTRAAMIFP 32
PVTKT VTPGSW EN L K GD + + V FP
Sbjct: 466 PVTKT*VTPGSWTENGLGVVKDGDLLITKMHNAVNAATRKTFP 338
Score = 60.2 bits (125), Expect(2) = 3e-059
Identities = 25/32 (78%), Positives = 27/32 (84%)
Frame = +1 / +3
Query: 427 ASQVNKIFAENAFDAVMHFAAXAYVGESTLEP 522
A V+KIF+EN FDAVMHFAA AYVGESTL P
Sbjct: 636 AKSVDKIFSENKFDAVMHFAAVAYVGESTLYP 731
Score = 49.6 bits (102), Expect(2) = 1e-040
Identities = 23/29 (79%), Positives = 24/29 (82%)
Frame = -3 / -3
Query: 521 GSNVLSPT*AXAAKCITASNAFSANILLT 435
G +VLSPT*A AAKCITASN FS NIL T
Sbjct: 730 G*SVLSPT*ATAAKCITASNLFSENILST 644
>gb|BI308597.1|BI308597 EST530007 GPOD Medicago truncatula cDNA clone pGPOD-7G10 5' end,
mRNA sequence
Length = 698
Score = 187 bits (402), Expect(2) = 2e-058
Identities = 79/112 (70%), Positives = 90/112 (80%)
Frame = +3 / +3
Query: 6 DARRKPRNVGKIIAALVLTTLCIFALKQSPGFGGNSVFSRHEPGVTHVLVTGGAGYIGSH 185
D ++K +GK+ LT LCI +K+SP F S FS HEPGVTHVLVTGGAGYIGS+
Sbjct: 246 DPKKKGNLLGKVFLVAALTALCILVIKRSPSFTTPSPFSVHEPGVTHVLVTGGAGYIGSY 425
Query: 186 AALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFPQPGRLQFIFADLGDQKSV 341
A LRLL++SYRVTIVDNLSRGN+GAV+VLQ LFPQPGRLQFI ADLGD KSV
Sbjct: 426 ATLRLLKESYRVTIVDNLSRGNLGAVRVLQSLFPQPGRLQFIHADLGDAKSV 581
Score = 133 bits (284), Expect(2) = 3e-039
Identities = 63/103 (61%), Positives = 69/103 (66%)
Frame = -1 / -2
Query: 340 TDFWSPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLSTMVTR*LSCKSLKAACEPI*PAP 161
TDF SP+SA +N SLPGCGNK RT TAP FP DRLSTMVTR* S +S K A +PI*PAP
Sbjct: 580 TDFASPKSACIN*SLPGCGNKL*RTLTAPKFPRDRLSTMVTR*DSFRSRKVAYDPI*PAP 401
Query: 160 PVTKTWVTPGSWRENTLLPPKPGDCFRANIQSVVRTRAAMIFP 32
PVTKT VTPGSW EN L K GD + + V FP
Sbjct: 400 PVTKT*VTPGSWTENGLGVVKDGDLLITKMHNAVNAATRKTFP 272
Score = 60.2 bits (125), Expect(2) = 2e-058
Identities = 25/32 (78%), Positives = 27/32 (84%)
Frame = +1 / +3
Query: 427 ASQVNKIFAENAFDAVMHFAAXAYVGESTLEP 522
A V+KIF+EN FDAVMHFAA AYVGESTL P
Sbjct: 570 AKSVDKIFSENKFDAVMHFAAVAYVGESTLYP 665
Score = 49.6 bits (102), Expect(2) = 3e-039
Identities = 23/29 (79%), Positives = 24/29 (82%)
Frame = -3 / -2
Query: 521 GSNVLSPT*AXAAKCITASNAFSANILLT 435
G +VLSPT*A AAKCITASN FS NIL T
Sbjct: 664 G*SVLSPT*ATAAKCITASNLFSENILST 578
>gb|AW691884.1|AW691884 NF045B08ST1F1000 Developing stem Medicago truncatula cDNA clone
NF045B08ST 5', mRNA sequence
Length = 660
Score = 192 bits (413), Expect(2) = 2e-055
Identities = 80/113 (70%), Positives = 94/113 (83%)
Frame = +3 / +2
Query: 6 DARRKPRNVGKIIAALVLTTLCIFALKQSPGFGGNSVFSRHEPGVTHVLVTGGAGYIGSH 185
D ++K VGKI A LT+LCI +K+SP S F+ HEPGVTHVLVTGGAGYIGSH
Sbjct: 245 DPKKKGNFVGKIFLAAALTSLCIIMIKRSPSLKSPSPFAFHEPGVTHVLVTGGAGYIGSH 424
Query: 186 AALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFPQPGRLQFIFADLGDQKSVS 344
AA RLL+D+YRVTIVDNLSRGN+GAV+VLQ LFP+PGRLQFI+ADLGD+KSV+
Sbjct: 425 AAFRLLKDNYRVTIVDNLSRGNLGAVRVLQDLFPEPGRLQFIYADLGDKKSVN 583
Score = 141 bits (303), Expect(2) = 3e-038
Identities = 70/112 (62%), Positives = 75/112 (66%)
Frame = -1 / -1
Query: 340 TDFWSPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLSTMVTR*LSCKSLKAACEPI*PAP 161
TDF SP+SA +N SLPG GN+ RT TAP FP DRLSTMVTR*LS + KAACEPI*PAP
Sbjct: 579 TDFLSPKSAYIN*SLPGSGNRS*RTLTAPKFPRDRLSTMVTR*LSFRRRKAACEPI*PAP 400
Query: 160 PVTKTWVTPGSWRENTLLPPKPGDCFRANIQSVVRTRAAMIFPTLRGFRLAS 5
PVTKT VTPGSW+ N L K GD I S V A IFPT F S
Sbjct: 399 PVTKT*VTPGSWKANGLGDFKDGDRLIMIIHSDVSAAARKIFPTKLPFFFGS 244
Score = 45.1 bits (92), Expect(2) = 2e-055
Identities = 18/25 (72%), Positives = 21/25 (84%)
Frame = +1 / +3
Query: 442 KIFAENAFDAVMHFAAXAYVGESTL 516
+ F +N FDAVMHFAA AYVGEST+
Sbjct: 585 RYFWKNKFDAVMHFAAVAYVGESTV 659
Score = 37.7 bits (76), Expect(2) = 3e-038
Identities = 17/25 (68%), Positives = 18/25 (72%)
Frame = -3 / -3
Query: 512 VLSPT*AXAAKCITASNAFSANILL 438
VLSPT A AAKCITASN F L+
Sbjct: 655 VLSPTYATAAKCITASNLFFQKYLI 581
>gb|AW774471.1|AW774471 EST333622 KV3 Medicago truncatula cDNA clone pKV3-22N3, mRNA
sequence
Length = 646
Score = 164 bits (352), Expect(2) = 1e-051
Identities = 70/84 (83%), Positives = 74/84 (88%)
Frame = +3 / +2
Query: 90 SPGFGGNSVFSRHEPGVTHVLVTGGAGYIGSHAALRLLQDSYRVTIVDNLSRGNMGAVKV 269
SP F S FS HEPGVTHVLVTGGAGYIGSHA LRLL++SYRVTIVDNLSRGN+GAV+V
Sbjct: 5 SPSFTTPSPFSVHEPGVTHVLVTGGAGYIGSHATLRLLKESYRVTIVDNLSRGNLGAVRV 184
Query: 270 LQGLFPQPGRLQFIFADLGDQKSV 341
LQ LFPQPGRLQFI ADLGD KSV
Sbjct: 185 LQSLFPQPGRLQFIHADLGDAKSV 256
Score = 133 bits (284), Expect(2) = 3e-039
Identities = 61/84 (72%), Positives = 65/84 (77%)
Frame = -1 / -2
Query: 340 TDFWSPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLSTMVTR*LSCKSLKAACEPI*PAP 161
TDF SP+SA +N SLPGCGNK RT TAP FP DRLSTMVTR* S +S K AC+PI*PAP
Sbjct: 255 TDFASPKSACIN*SLPGCGNKL*RTLTAPKFPRDRLSTMVTR*DSFRSRKVACDPI*PAP 76
Query: 160 PVTKTWVTPGSWRENTLLPPKPGD 89
PVTKT VTPGSW EN L K GD
Sbjct: 75 PVTKT*VTPGSWTENGLGVVKDGD 4
Score = 60.2 bits (125), Expect(2) = 1e-051
Identities = 25/32 (78%), Positives = 27/32 (84%)
Frame = +1 / +2
Query: 427 ASQVNKIFAENAFDAVMHFAAXAYVGESTLEP 522
A V+KIF+EN FDAVMHFAA AYVGESTL P
Sbjct: 245 AKSVDKIFSENKFDAVMHFAAVAYVGESTLYP 340
Score = 49.6 bits (102), Expect(2) = 3e-039
Identities = 23/29 (79%), Positives = 24/29 (82%)
Frame = -3 / -2
Query: 521 GSNVLSPT*AXAAKCITASNAFSANILLT 435
G +VLSPT*A AAKCITASN FS NIL T
Sbjct: 339 G*SVLSPT*ATAAKCITASNLFSENILST 253
>gb|BI272466.1|BI272466 NF021H08FL1F1075 Developing flower Medicago truncatula cDNA clone
NF021H08FL 5', mRNA sequence
Length = 713
Score = 137 bits (293), Expect(3) = 8e-049
Identities = 56/66 (84%), Positives = 64/66 (96%)
Frame = +3 / +1
Query: 147 VLVTGGAGYIGSHAALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFPQPGRLQFIFADLG 326
VLVTGGAGYIGSHAA RLL+D+YRVTIVDNLSRGN+GAV+VLQ LFP+PGRLQFI+ADLG
Sbjct: 85 VLVTGGAGYIGSHAAFRLLKDNYRVTIVDNLSRGNLGAVRVLQDLFPEPGRLQFIYADLG 264
Query: 327 DQKSVS 344
D+KSV+
Sbjct: 265 DKKSVN 282
Score = 109 bits (232), Expect(3) = 6e-036
Identities = 49/65 (75%), Positives = 53/65 (81%)
Frame = -1 / -1
Query: 340 TDFWSPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLSTMVTR*LSCKSLKAACEPI*PAP 161
TDF SP+SA +N SLPG GN+ RT TAP FP DRLSTMVTR*LS + KAACEPI*PAP
Sbjct: 278 TDFLSPKSAYIN*SLPGSGNRS*RTLTAPKFPRDRLSTMVTR*LSFRRRKAACEPI*PAP 99
Query: 160 PVTKT 146
PVTKT
Sbjct: 98 PVTKT 84
Score = 60.2 bits (125), Expect(3) = 8e-049
Identities = 24/29 (82%), Positives = 26/29 (89%)
Frame = +1 / +1
Query: 436 VNKIFAENAFDAVMHFAAXAYVGESTLEP 522
VNKIF EN FDAVMHFAA AYVGEST++P
Sbjct: 277 VNKIFLENKFDAVMHFAAVAYVGESTVDP 363
Score = 53.3 bits (110), Expect(3) = 6e-036
Identities = 24/29 (82%), Positives = 24/29 (82%)
Frame = -3 / -1
Query: 521 GSNVLSPT*AXAAKCITASNAFSANILLT 435
GS VLSPT A AAKCITASN FS NILLT
Sbjct: 362 GSTVLSPTYATAAKCITASNLFSKNILLT 276
Score = 35.9 bits (72), Expect(3) = 8e-049
Identities = 14/26 (53%), Positives = 17/26 (65%)
Frame = +3 / +2
Query: 66 LCIFALKQSPGFGGNSVFSRHEPGVT 143
LCI +K+SP S F+ HEPGVT
Sbjct: 2 LCIIMIKRSPSLKSPSPFAFHEPGVT 79
Score = 27.2 bits (53), Expect(3) = 6e-036
Identities = 11/18 (61%), Positives = 12/18 (66%)
Frame = -1 / -3
Query: 142 VTPGSWRENTLLPPKPGD 89
VTPGSW+ N L K GD
Sbjct: 78 VTPGSWKANGLGDFKDGD 25
>gb|AW574031.1|AW574031 EST316622 GVN Medicago truncatula cDNA clone pGVN-50L10, mRNA
sequence
Length = 699
Score = 189 bits (408), Expect = 2e-047
Identities = 80/112 (71%), Positives = 90/112 (80%)
Frame = +3 / +2
Query: 6 DARRKPRNVGKIIAALVLTTLCIFALKQSPGFGGNSVFSRHEPGVTHVLVTGGAGYIGSH 185
D ++K +GK+ LT LCI +K+SP F S FS HEPGVTHVLVTGGAGYIGSH
Sbjct: 332 DPKKKGNLLGKVFLVAALTALCILVIKRSPSFTTPSPFSVHEPGVTHVLVTGGAGYIGSH 511
Query: 186 AALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFPQPGRLQFIFADLGDQKSV 341
A LRLL++SYRVTIVDNLSRGN+GAV+VLQ LFPQPGRLQFI ADLGD KSV
Sbjct: 512 ATLRLLKESYRVTIVDNLSRGNLGAVRVLQSLFPQPGRLQFIHADLGDAKSV 667
Score = 138 bits (295), Expect = 8e-032
Identities = 64/103 (62%), Positives = 70/103 (67%)
Frame = -1 / -1
Query: 340 TDFWSPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLSTMVTR*LSCKSLKAACEPI*PAP 161
TDF SP+SA +N SLPGCGNK RT TAP FP DRLSTMVTR* S +S K AC+PI*PAP
Sbjct: 666 TDFASPKSACIN*SLPGCGNKL*RTLTAPKFPRDRLSTMVTR*DSFRSRKVACDPI*PAP 487
Query: 160 PVTKTWVTPGSWRENTLLPPKPGDCFRANIQSVVRTRAAMIFP 32
PVTKT VTPGSW EN L K GD + + V FP
Sbjct: 486 PVTKT*VTPGSWTENGLGVVKDGDLLITKMHNAVNAATRKTFP 358
>gb|AW686486.2|AW686486 NF041H10NR1F1000 Nodulated root Medicago truncatula cDNA clone
NF041H10NR 5', mRNA sequence
Length = 561
Score = 186 bits (400), Expect(2) = 3e-047
Identities = 78/110 (70%), Positives = 88/110 (80%)
Frame = +3 / +2
Query: 6 DARRKPRNVGKIIAALVLTTLCIFALKQSPGFGGNSVFSRHEPGVTHVLVTGGAGYIGSH 185
D ++K +GK+ LT LCI +K+SP F S FS HEPGVTHVLVTGGAGYIGSH
Sbjct: 176 DPKKKGNLLGKVFLVAALTALCILVIKRSPSFTTPSPFSVHEPGVTHVLVTGGAGYIGSH 355
Query: 186 AALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFPQPGRLQFIFADLGDQK 335
A LRLL++SYRVTIVDNLSRGN+GAV+VLQ LFPQPGRLQFI ADLGD K
Sbjct: 356 ATLRLLKESYRVTIVDNLSRGNLGAVRVLQSLFPQPGRLQFIHADLGDAK 505
Score = 134 bits (288), Expect = 8e-031
Identities = 65/116 (56%), Positives = 73/116 (62%)
Frame = -1 / -1
Query: 379 RHKQNSFRSIDEDTDFWSPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLSTMVTR*LSCK 200
+H+ R I F SP+SA +N SLPGCGNK RT TAP FP DRLSTMVTR* S +
Sbjct: 549 QHQTFILRKIFYQQIFASPKSACIN*SLPGCGNKL*RTLTAPKFPRDRLSTMVTR*DSFR 370
Query: 199 SLKAACEPI*PAPPVTKTWVTPGSWRENTLLPPKPGDCFRANIQSVVRTRAAMIFP 32
S K AC+PI*PAPPVTKT VTPGSW EN L K GD + + V FP
Sbjct: 369 SRKVACDPI*PAPPVTKT*VTPGSWTENGLGVVKDGDLLITKMHNAVNAATRKTFP 202
Score = 23.5 bits (45), Expect(2) = 3e-047
Identities = 9/12 (75%), Positives = 9/12 (75%)
Frame = +1 / +3
Query: 448 FAENAFDAVMHF 483
F E FDAVMHF
Sbjct: 525 FGE*RFDAVMHF 560
>gb|AC161863.20| Medicago truncatula clone mth2-175f4, complete sequence
Length = 116075
Score = 82.2 bits (173), Expect(3) = 3e-046
Identities = 33/38 (86%), Positives = 36/38 (94%)
Frame = +3 / -3
Query: 117 FSRHEPGVTHVLVTGGAGYIGSHAALRLLQDSYRVTIV 230
F+ HEPGVTHVLVTGGAGYIGSHAA RLL+D+YRVTIV
Sbjct: 19659 FAFHEPGVTHVLVTGGAGYIGSHAAFRLLKDNYRVTIV 19546
Score = 80.8 bits (170), Expect(3) = 3e-046
Identities = 32/38 (84%), Positives = 37/38 (97%)
Frame = +3 / -3
Query: 231 DNLSRGNMGAVKVLQGLFPQPGRLQFIFADLGDQKSVS 344
DNLSRGN+GAV+VLQ LFP+PGRLQFI+ADLGD+KSVS
Sbjct: 19443 DNLSRGNLGAVRVLQDLFPEPGRLQFIYADLGDKKSVS 19330
Score = 74.4 bits (156), Expect(3) = 2e-035
Identities = 32/41 (78%), Positives = 35/41 (85%)
Frame = -1 / +2
Query: 238 RLSTMVTR*LSCKSLKAACEPI*PAPPVTKTWVTPGSWREN 116
R +TMVTR*LS + KAACEPI*PAPPVTKT VTPGSW+ N
Sbjct: 19538 RHTTMVTR*LSFRRRKAACEPI*PAPPVTKT*VTPGSWKAN 19660
Score = 61.6 bits (128), Expect(3) = 3e-046
Identities = 26/34 (76%), Positives = 28/34 (82%)
Frame = +1 / -1
Query: 421 LTASQVNKIFAENAFDAVMHFAAXAYVGESTLEP 522
LT VNKIF EN FDAVMHFAA AYVGEST++P
Sbjct: 19244 LTGY*VNKIFLENKFDAVMHFAAVAYVGESTVDP 19143
Score = 60.2 bits (125), Expect(3) = 2e-035
Identities = 26/38 (68%), Positives = 29/38 (76%)
Frame = -1 / +2
Query: 343 DTDFWSPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLS 230
DTDF SP+SA +N SLPG GN+ RT TAP FP DRLS
Sbjct: 19331 DTDFLSPKSAYIN*SLPGSGNRS*RTLTAPKFPRDRLS 19444
Score = 53.3 bits (110), Expect(3) = 2e-035
Identities = 24/29 (82%), Positives = 24/29 (82%)
Frame = -3 / +1
Query: 521 GSNVLSPT*AXAAKCITASNAFSANILLT 435
GS VLSPT A AAKCITASN FS NILLT
Sbjct: 19144 GSTVLSPTYATAAKCITASNLFSKNILLT 19230
>gb|BF646657.1|BF646657 NF076H12EC1F1103 Elicited cell culture Medicago truncatula cDNA
clone NF076H12EC 5', mRNA sequence
Length = 562
Score = 181 bits (389), Expect = 9e-045
Identities = 76/108 (70%), Positives = 86/108 (79%)
Frame = +3 / +1
Query: 6 DARRKPRNVGKIIAALVLTTLCIFALKQSPGFGGNSVFSRHEPGVTHVLVTGGAGYIGSH 185
D ++K +GK+ LT LCI +K+SP F S FS HEPGVTHVLVTGGA YIGSH
Sbjct: 235 DPKKKGNLLGKVFLVAALTALCILVIKRSPSFTTPSPFSVHEPGVTHVLVTGGAXYIGSH 414
Query: 186 AALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFPQPGRLQFIFADLGD 329
A LRLL++SYRVTIVDNLSRGN+GAV+VLQ LFPQPGRLQFI ADLGD
Sbjct: 415 ATLRLLKESYRVTIVDNLSRGNLGAVRVLQSLFPQPGRLQFIHADLGD 558
Score = 127 bits (272), Expect = 1e-028
Identities = 60/99 (60%), Positives = 66/99 (66%)
Frame = -1 / -3
Query: 328 SPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLSTMVTR*LSCKSLKAACEPI*PAPPVTK 149
SP+SA +N SLPGCGNK RT TAP FP DRLSTMVTR* S +S K AC+PI* APPVTK
Sbjct: 557 SPKSACIN*SLPGCGNKL*RTLTAPKFPRDRLSTMVTR*DSFRSRKVACDPI*XAPPVTK 378
Query: 148 TWVTPGSWRENTLLPPKPGDCFRANIQSVVRTRAAMIFP 32
T VTPGSW EN L K GD + + V FP
Sbjct: 377 T*VTPGSWTENGLGVVKDGDLLITKMHNAVNAATRKTFP 261
>gb|BE204599.1|BE204599 EST397275 KV0 Medicago truncatula cDNA clone pKV0-16F14, mRNA
sequence
Length = 525
Score = 171 bits (369), Expect = 5e-042
Identities = 72/102 (70%), Positives = 83/102 (81%)
Frame = +3 / +1
Query: 6 DARRKPRNVGKIIAALVLTTLCIFALKQSPGFGGNSVFSRHEPGVTHVLVTGGAGYIGSH 185
D ++K VGKI A LT+LCI +K+SP S F+ HEPGVTHVLVTGGAGYIGSH
Sbjct: 220 DPKKKGNFVGKIFLAAALTSLCIIMIKRSPSLKSPSPFAFHEPGVTHVLVTGGAGYIGSH 399
Query: 186 AALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFPQPGRLQFI 311
AA RLL+D+YRVTIVDNLSRGN+GAV+VLQ LFP+PGRLQFI
Sbjct: 400 AAFRLLKDNYRVTIVDNLSRGNLGAVRVLQDLFPEPGRLQFI 525
Score = 126 bits (269), Expect = 3e-028
Identities = 63/102 (61%), Positives = 67/102 (65%)
Frame = -1 / -2
Query: 310 MNCSLPGCGNKPCRTFTAPIFPLDRLSTMVTR*LSCKSLKAACEPI*PAPPVTKTWVTPG 131
+N SLPG GN+ RT TAP FP DRLSTMVTR*LS + KAACEPI*PAPPVTKT VTPG
Sbjct: 524 IN*SLPGSGNRS*RTLTAPKFPRDRLSTMVTR*LSFRRRKAACEPI*PAPPVTKT*VTPG 345
Query: 130 SWRENTLLPPKPGDCFRANIQSVVRTRAAMIFPTLRGFRLAS 5
SW+ N L K GD I S V A IFPT F S
Sbjct: 344 SWKANGLGDFKDGDRLIMIIHSDVSAAARKIFPTKLPFFFGS 219
>gb|BG582283.1|BG582283 EST484024 GVN Medicago truncatula cDNA clone pGVN-69A4 5' end, mRNA
sequence
Length = 388
Score = 116 bits (247), Expect(2) = 2e-037
Identities = 47/57 (82%), Positives = 55/57 (96%)
Frame = +3 / +3
Query: 174 IGSHAALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFPQPGRLQFIFADLGDQKSVS 344
IGSHAA RLL+D+YRVTIVDNLSRGN+GAV+VLQ LFP+PGRLQFI+ADLGD+KSV+
Sbjct: 15 IGSHAAFRLLKDNYRVTIVDNLSRGNLGAVRVLQDLFPEPGRLQFIYADLGDKKSVN 185
Score = 88.6 bits (187), Expect(2) = 3e-027
Identities = 40/56 (71%), Positives = 44/56 (78%)
Frame = -1 / -1
Query: 340 TDFWSPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLSTMVTR*LSCKSLKAACEPI 173
TDF SP+SA +N SLPG GN+ RT TAP FP DRLSTMVTR*LS + KAACEPI
Sbjct: 181 TDFLSPKSAYIN*SLPGSGNRS*RTLTAPKFPRDRLSTMVTR*LSFRRRKAACEPI 14
Score = 60.2 bits (125), Expect(2) = 2e-037
Identities = 24/29 (82%), Positives = 26/29 (89%)
Frame = +1 / +3
Query: 436 VNKIFAENAFDAVMHFAAXAYVGESTLEP 522
VNKIF EN FDAVMHFAA AYVGEST++P
Sbjct: 180 VNKIFLENKFDAVMHFAAVAYVGESTVDP 266
Score = 53.3 bits (110), Expect(2) = 3e-027
Identities = 24/29 (82%), Positives = 24/29 (82%)
Frame = -3 / -1
Query: 521 GSNVLSPT*AXAAKCITASNAFSANILLT 435
GS VLSPT A AAKCITASN FS NILLT
Sbjct: 265 GSTVLSPTYATAAKCITASNLFSKNILLT 179
>gb|BE321417.1|BE321417 NF024H12IN1F1096 Insect herbivory Medicago truncatula cDNA clone
NF024H12IN 5', mRNA sequence
Length = 644
Score = 136 bits (291), Expect(2) = 2e-036
Identities = 57/84 (67%), Positives = 66/84 (78%)
Frame = +3 / +2
Query: 6 DARRKPRNVGKIIAALVLTTLCIFALKQSPGFGGNSVFSRHEPGVTHVLVTGGAGYIGSH 185
D ++K VGKI A LT+LCI +K+SP S F+ HEPGVTHVLVTGGAGYIGSH
Sbjct: 335 DPKKKGNFVGKIFLAAALTSLCIIMIKRSPSLKSPSPFAFHEPGVTHVLVTGGAGYIGSH 514
Query: 186 AALRLLQDSYRVTIVDNLSRGNMG 257
AA RLL+D+YRVTIVDNLSRGN+G
Sbjct: 515 AAFRLLKDNYRVTIVDNLSRGNLG 586
Score = 102 bits (218), Expect(2) = 9e-024
Identities = 52/84 (61%), Positives = 54/84 (64%)
Frame = -1 / -3
Query: 256 PIFPLDRLSTMVTR*LSCKSLKAACEPI*PAPPVTKTWVTPGSWRENTLLPPKPGDCFRA 77
P FP DRLSTMVTR*LS + KAACEPI*PAPPVTKT VTPGSW+ N L K GD
Sbjct: 585 PKFPRDRLSTMVTR*LSFRRRKAACEPI*PAPPVTKT*VTPGSWKANGLGDFKDGDRLIM 406
Query: 76 NIQSVVRTRAAMIFPTLRGFRLAS 5
I S V A IFPT F S
Sbjct: 405 IIHSDVSAAARKIFPTKLPFFFGS 334
Score = 36.8 bits (74), Expect(2) = 2e-036
Identities = 14/17 (82%), Positives = 16/17 (94%)
Frame = +3 / +3
Query: 255 GAVKVLQGLFPQPGRLQ 305
GAV+VLQ LFP+PGRLQ
Sbjct: 585 GAVRVLQDLFPEPGRLQ 635
Score = 28.1 bits (55), Expect(2) = 9e-024
Identities = 11/16 (68%), Positives = 12/16 (75%)
Frame = -1 / -2
Query: 301 SLPGCGNKPCRTFTAP 254
SLPG GN+ RT TAP
Sbjct: 631 SLPGSGNRS*RTLTAP 584
>gb|BE942980.1|BE942980 EST422559 MGHG Medicago truncatula cDNA clone pMGHG-14M15, mRNA
sequence
Length = 461
Score = 108 bits (231), Expect(2) = 4e-035
Identities = 45/55 (81%), Positives = 50/55 (90%)
Frame = +3 / +3
Query: 177 GSHAALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFPQPGRLQFIFADLGDQKSV 341
G HA LRL+++SYRVTIVDNLSRGN+GAV+VLQ LFPQPGRLQFI ADLGD KSV
Sbjct: 18 GHHATLRLVKESYRVTIVDNLSRGNLGAVRVLQSLFPQPGRLQFIHADLGDAKSV 182
Score = 76.7 bits (161), Expect(2) = 2e-022
Identities = 37/52 (71%), Positives = 39/52 (75%)
Frame = -1 / -2
Query: 340 TDFWSPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLSTMVTR*LSCKSLKAA 185
TDF SP+SA +N SLPGCGNK RT TAP FP DRLSTMVTR* S S K A
Sbjct: 181 TDFASPKSACIN*SLPGCGNKL*RTLTAPKFPRDRLSTMVTR*DSFTSRKVA 26
Score = 60.2 bits (125), Expect(2) = 4e-035
Identities = 25/32 (78%), Positives = 27/32 (84%)
Frame = +1 / +3
Query: 427 ASQVNKIFAENAFDAVMHFAAXAYVGESTLEP 522
A V+KIF+EN FDAVMHFAA AYVGESTL P
Sbjct: 171 AKSVDKIFSENKFDAVMHFAAVAYVGESTLYP 266
Score = 49.6 bits (102), Expect(2) = 2e-022
Identities = 23/29 (79%), Positives = 24/29 (82%)
Frame = -3 / -2
Query: 521 GSNVLSPT*AXAAKCITASNAFSANILLT 435
G +VLSPT*A AAKCITASN FS NIL T
Sbjct: 265 G*SVLSPT*ATAAKCITASNLFSENILST 179
>gb|AW776261.1|AW776261 EST335326 DSIL Medicago truncatula cDNA clone pDSIL-7E2, mRNA
sequence
Length = 508
Score = 142 bits (304), Expect = 5e-033
Identities = 59/89 (66%), Positives = 70/89 (78%)
Frame = +3 / +2
Query: 6 DARRKPRNVGKIIAALVLTTLCIFALKQSPGFGGNSVFSRHEPGVTHVLVTGGAGYIGSH 185
D ++K +GK+ LT LCI +K+SP F S FS HEPGVTHVLVTGGAGYIGSH
Sbjct: 236 DPKKKGNLLGKVFLVAALTALCILVIKRSPSFTTPSPFSVHEPGVTHVLVTGGAGYIGSH 415
Query: 186 AALRLLQDSYRVTIVDNLSRGNMGAVKVL 272
A LRLL++SYRVTI+DNLSRGN+GAV+VL
Sbjct: 416 ATLRLLKESYRVTILDNLSRGNLGAVRVL 502
>emb|CR486384.1| mth2-155D12FM2 BAC end, cultivar Jemalong A17 of Medicago
truncatula, genomic survey sequence
Length = 702
Score = 83.1 bits (175), Expect(4) = 8e-033
Identities = 34/38 (89%), Positives = 36/38 (94%)
Frame = +3 / +1
Query: 117 FSRHEPGVTHVLVTGGAGYIGSHAALRLLQDSYRVTIV 230
FS HEPGVTHVLVTGGAGYIGSHA LRLL++SYRVTIV
Sbjct: 397 FSVHEPGVTHVLVTGGAGYIGSHATLRLLKESYRVTIV 510
Score = 39.6 bits (80), Expect(4) = 8e-033
Identities = 15/16 (93%), Positives = 15/16 (93%)
Frame = +3 / +1
Query: 282 FPQPGRLQFIFADLGD 329
FPQPGRLQFI ADLGD
Sbjct: 655 FPQPGRLQFIHADLGD 702
Score = 38.6 bits (78), Expect(4) = 8e-033
Identities = 15/18 (83%), Positives = 17/18 (94%)
Frame = +3 / +3
Query: 231 DNLSRGNMGAVKVLQGLF 284
DNLSRGN+GAV+VLQ LF
Sbjct: 603 DNLSRGNLGAVRVLQSLF 656
Score = 36.3 bits (73), Expect(4) = 8e-033
Identities = 13/32 (40%), Positives = 19/32 (59%)
Frame = +3 / +1
Query: 6 DARRKPRNVGKIIAALVLTTLCIFALKQSPGF 101
D ++K +GK+ LT LCI +K+SP F
Sbjct: 58 DPKKKGNLLGKVFLVAALTALCILVIKRSPSF 153
>gb|BE321428.1|BE321428 NF025H12IN1F1096 Insect herbivory Medicago truncatula cDNA clone
NF025H12IN 5', mRNA sequence
Length = 646
Score = 73.0 bits (153), Expect(3) = 9e-029
Identities = 31/54 (57%), Positives = 37/54 (68%)
Frame = +3 / +2
Query: 6 DARRKPRNVGKIIAALVLTTLCIFALKQSPGFGGNSVFSRHEPGVTHVLVTGGA 167
D ++K VGKI A LT+LCI +K+SP S F+ HEPGVTHVLVTGGA
Sbjct: 335 DPKKKGNFVGKIFLAAALTSLCIIMIKRSPSLKSPSPFAFHEPGVTHVLVTGGA 496
Score = 56.1 bits (116), Expect(3) = 9e-029
Identities = 23/29 (79%), Positives = 26/29 (89%)
Frame = +3 / +3
Query: 159 GGAGYIGSHAALRLLQDSYRVTIVDNLSR 245
G GYIGSHAA RLL+D+YRVTIVDNLS+
Sbjct: 489 GVQGYIGSHAAFRLLKDNYRVTIVDNLSQ 575
Score = 36.3 bits (73), Expect(3) = 9e-029
Identities = 13/23 (56%), Positives = 19/23 (82%)
Frame = +3 / +2
Query: 234 NLSRGNMGAVKVLQGLFPQPGRL 302
+++ N+GAV+VL LFP+PGRL
Sbjct: 566 SVTERNLGAVRVLSDLFPEPGRL 634
>gb|CX527137.1|CX527137 s13dNF40D04AT042_514786 Aphid-Infected Shoots Medicago truncatula
cDNA, mRNA sequence
Length = 617
Score = 81.7 bits (172), Expect(2) = 5e-027
Identities = 32/42 (76%), Positives = 40/42 (95%)
Frame = +3 / +3
Query: 219 VTIVDNLSRGNMGAVKVLQGLFPQPGRLQFIFADLGDQKSVS 344
+T+ DNLSRGN+GAV+VLQ LFP+PGRLQFI+ADLGD+KSV+
Sbjct: 267 LTLRDNLSRGNLGAVRVLQDLFPEPGRLQFIYADLGDKKSVN 392
Score = 60.2 bits (125), Expect(2) = 5e-027
Identities = 24/29 (82%), Positives = 26/29 (89%)
Frame = +1 / +3
Query: 436 VNKIFAENAFDAVMHFAAXAYVGESTLEP 522
VNKIF EN FDAVMHFAA AYVGEST++P
Sbjct: 387 VNKIFLENKFDAVMHFAAVAYVGESTVDP 473
>gb|DW016466.1|DW016466 EST1225427 MTY Medicago truncatula cDNA clone MTYAH55, mRNA
sequence
Length = 574
Score = 102 bits (217), Expect(2) = 6e-027
Identities = 41/46 (89%), Positives = 45/46 (97%)
Frame = +3 / +2
Query: 126 HEPGVTHVLVTGGAGYIGSHAALRLLQDSYRVTIVDNLSRGNMGAV 263
HEPGVTHVLVTGGAGYIGSHA L+LL++SYRVTIVDNLSRGN+GAV
Sbjct: 437 HEPGVTHVLVTGGAGYIGSHATLQLLKESYRVTIVDNLSRGNLGAV 574
Score = 39.1 bits (79), Expect(2) = 6e-027
Identities = 16/39 (41%), Positives = 22/39 (56%)
Frame = +3 / +3
Query: 6 DARRKPRNVGKIIAALVLTTLCIFALKQSPGFGGNSVFS 122
D ++K +GK+ LT LCI +K+SP F S FS
Sbjct: 318 DPKKKGNLLGKVFLVAALTALCILVIKRSPSFTTPSPFS 434
>gb|BI271915.1|BI271915 NF016C03FL1F1021 Developing flower Medicago truncatula cDNA clone
NF016C03FL 5', mRNA sequence
Length = 364
Score = 60.2 bits (125), Expect(3) = 3e-025
Identities = 24/29 (82%), Positives = 26/29 (89%)
Frame = +1 / +2
Query: 436 VNKIFAENAFDAVMHFAAXAYVGESTLEP 522
VNKIF EN FDAVMHFAA AYVGEST++P
Sbjct: 146 VNKIFLENKFDAVMHFAAVAYVGESTVDP 232
Score = 57.0 bits (118), Expect(3) = 3e-025
Identities = 22/28 (78%), Positives = 27/28 (96%)
Frame = +3 / +2
Query: 261 VKVLQGLFPQPGRLQFIFADLGDQKSVS 344
V+VLQ LFP+PGRLQFI+ADLGD+KSV+
Sbjct: 68 VRVLQDLFPEPGRLQFIYADLGDKKSVN 151
Score = 35.0 bits (70), Expect(3) = 3e-025
Identities = 14/22 (63%), Positives = 18/22 (81%)
Frame = +3 / +3
Query: 198 LLQDSYRVTIVDNLSRGNMGAV 263
LL+D+YRVTIVDNLS +G +
Sbjct: 3 LLKDNYRVTIVDNLSPRKLGCL 68
>gb|CX527706.1|CX527706 s13dNF38E01AT006_515924 Aphid-Infected Shoots Medicago truncatula
cDNA, mRNA sequence
Length = 552
Score = 113 bits (242), Expect = 2e-024
Identities = 48/74 (64%), Positives = 56/74 (75%)
Frame = +3 / +2
Query: 6 DARRKPRNVGKIIAALVLTTLCIFALKQSPGFGGNSVFSRHEPGVTHVLVTGGAGYIGSH 185
D ++K VGKI A LT+LCI +K+SP S F+ HEPGVTHVLVTGGAGYIGSH
Sbjct: 329 DPKKKGNFVGKIFLAAALTSLCIIMIKRSPSLKSPSPFAFHEPGVTHVLVTGGAGYIGSH 508
Query: 186 AALRLLQDSYRVTI 227
AA RLL+D+YRVTI
Sbjct: 509 AAFRLLKDNYRVTI 550
>gb|BF650120.1|BF650120 NF088C04EC1F1024 Elicited cell culture Medicago truncatula cDNA
clone NF088C04EC 5', mRNA sequence
Length = 619
Score = 109 bits (233), Expect = 3e-023
Identities = 46/75 (61%), Positives = 54/75 (72%)
Frame = +3 / +2
Query: 6 DARRKPRNVGKIIAALVLTTLCIFALKQSPGFGGNSVFSRHEPGVTHVLVTGGAGYIGSH 185
D ++K +GK+ LT LCI +K+SP F S S HEPGVTHVLVTGGAGYIGSH
Sbjct: 362 DPKKKGNLLGKVFLVAALTALCILVIKRSPSFTTPSPXSVHEPGVTHVLVTGGAGYIGSH 541
Query: 186 AALRLLQDSYRVTIV 230
A LRLL++ YRVTIV
Sbjct: 542 ATLRLLKEXYRVTIV 586
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 537,721,208
Number of Sequences: 392609
Number of extensions: 7569159
Number of successful extensions: 54083
Number of sequences better than 1.0e-020: 26
length of database: 147,244,331
effective HSP length: 53
effective length of database: 126,436,054
effective search space used: 15172326480
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)