TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCA13d08.yg.2.1
(511 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AC174291.5| Medicago truncatula clone mth2-58k14, WORKIN... 79 2e-034
gb|AC174291.6| Medicago truncatula clone mth2-58k14, WORKIN... 79 2e-034
gb|AC151528.35| Medicago truncatula clone mth2-135j6, WORKI... 79 5e-034
gb|AC152156.30| Medicago truncatula clone mth2-136k22, WORK... 79 1e-033
gb|AC171168.2| Medicago truncatula chromosome 2 clone mth2-... 68 5e-029
emb|CR338322.1| mte1-7D21RM1 BAC end, cultivar Jemalong A17... 79 6e-024
>gb|AC174291.5| Medicago truncatula clone mth2-58k14, WORKING DRAFT SEQUENCE, 12
unordered pieces
Length = 113413
Score = 79.4 bits (167), Expect(3) = 2e-034
Identities = 28/47 (59%), Positives = 38/47 (80%)
Frame = +1 / -3
Query: 178 DVATKGHGGLGVFVGVCIISAIFGTADANCQGALVGDLSLMCPEFIQ 318
D+AT G GGLG F+G+CI+S +FG ADA QG ++GD+SLM P+F+Q
Sbjct: 53102 DLATSGKGGLGTFIGICIVSGVFGIADALAQGGMIGDISLMHPDFMQ 52962
Score = 66.1 bits (138), Expect(3) = 2e-034
Identities = 30/48 (62%), Positives = 36/48 (75%)
Frame = +1 / -2
Query: 313 IQSFMAGLAASGVLTSALRLVTKAAFESSKDGLRIGAILFFSITCLFE 456
+QSF+AG AASG LTS LRL+TKA FE+SKDGLR GA F ++ E
Sbjct: 52881 LQSFLAGEAASGALTSVLRLITKAIFENSKDGLRKGASKFNTLMLSIE 52738
Score = 58.3 bits (121), Expect(3) = 1e-025
Identities = 24/38 (63%), Positives = 27/38 (71%)
Frame = -3 / +2
Query: 431 NSIAPMRRPSFELSKAALVTNLKADVRTPDAASPAMKD 318
N +AP+RRPS E S A V NLK DV P+AASPA KD
Sbjct: 52763 NLLAPLRRPSLEFSNIAFVINLKTDVSAPEAASPARKD 52876
Score = 55.6 bits (115), Expect(3) = 2e-034
Identities = 22/30 (73%), Positives = 25/30 (83%)
Frame = +1 / -3
Query: 37 VLTLVYQPFAFGITLIMTYYEAKMNTRLRN 126
VLTLVYQPFAFG I+ Y+EAK+NTR RN
Sbjct: 53336 VLTLVYQPFAFGTMAILAYHEAKLNTRKRN 53247
Score = 53.3 bits (110), Expect(3) = 1e-025
Identities = 25/46 (54%), Positives = 27/46 (58%)
Frame = -3 / +1
Query: 314 MNSGHIKERSPTSAP*QLASAVPNMALIMHTPTKTPSPPCPLVATS 177
M SG IK+ SP P ASA+P L MH P K PSPP P VA S
Sbjct: 52966 MKSGCIKDISPIIPPCARASAIPKTPLTMHIPMKVPSPPFPDVAKS 53103
Score = 42.8 bits (87), Expect(3) = 1e-025
Identities = 22/47 (46%), Positives = 27/47 (57%)
Frame = -3 / +1
Query: 176 RIINAKEPRKKRENPARFLNLVFIFAS*YVMMRVIPKAKGW*TSVST 36
+I A E +KK P RF LV FAS + ++PKA GW T VST
Sbjct: 53197 KINTAIELKKKSVYPDRFRFLVLSFASW*ASIAIVPKANGWYTRVST 53337
>gb|AC174291.6| Medicago truncatula clone mth2-58k14, WORKING DRAFT SEQUENCE, 12
unordered pieces
Length = 113563
Score = 79.4 bits (167), Expect(3) = 2e-034
Identities = 28/47 (59%), Positives = 38/47 (80%)
Frame = +1 / -3
Query: 178 DVATKGHGGLGVFVGVCIISAIFGTADANCQGALVGDLSLMCPEFIQ 318
D+AT G GGLG F+G+CI+S +FG ADA QG ++GD+SLM P+F+Q
Sbjct: 53102 DLATSGKGGLGTFIGICIVSGVFGIADALAQGGMIGDISLMHPDFMQ 52962
Score = 66.1 bits (138), Expect(3) = 2e-034
Identities = 30/48 (62%), Positives = 36/48 (75%)
Frame = +1 / -2
Query: 313 IQSFMAGLAASGVLTSALRLVTKAAFESSKDGLRIGAILFFSITCLFE 456
+QSF+AG AASG LTS LRL+TKA FE+SKDGLR GA F ++ E
Sbjct: 52881 LQSFLAGEAASGALTSVLRLITKAIFENSKDGLRKGASKFNTLMLSIE 52738
Score = 58.3 bits (121), Expect(3) = 1e-025
Identities = 24/38 (63%), Positives = 27/38 (71%)
Frame = -3 / +2
Query: 431 NSIAPMRRPSFELSKAALVTNLKADVRTPDAASPAMKD 318
N +AP+RRPS E S A V NLK DV P+AASPA KD
Sbjct: 52763 NLLAPLRRPSLEFSNIAFVINLKTDVSAPEAASPARKD 52876
Score = 55.6 bits (115), Expect(3) = 2e-034
Identities = 22/30 (73%), Positives = 25/30 (83%)
Frame = +1 / -3
Query: 37 VLTLVYQPFAFGITLIMTYYEAKMNTRLRN 126
VLTLVYQPFAFG I+ Y+EAK+NTR RN
Sbjct: 53336 VLTLVYQPFAFGTMAILAYHEAKLNTRKRN 53247
Score = 53.3 bits (110), Expect(3) = 1e-025
Identities = 25/46 (54%), Positives = 27/46 (58%)
Frame = -3 / +1
Query: 314 MNSGHIKERSPTSAP*QLASAVPNMALIMHTPTKTPSPPCPLVATS 177
M SG IK+ SP P ASA+P L MH P K PSPP P VA S
Sbjct: 52966 MKSGCIKDISPIIPPCARASAIPKTPLTMHIPMKVPSPPFPDVAKS 53103
Score = 42.8 bits (87), Expect(3) = 1e-025
Identities = 22/47 (46%), Positives = 27/47 (57%)
Frame = -3 / +1
Query: 176 RIINAKEPRKKRENPARFLNLVFIFAS*YVMMRVIPKAKGW*TSVST 36
+I A E +KK P RF LV FAS + ++PKA GW T VST
Sbjct: 53197 KINTAIELKKKSVYPDRFRFLVLSFASW*ASIAIVPKANGWYTRVST 53337
>gb|AC151528.35| Medicago truncatula clone mth2-135j6, WORKING DRAFT SEQUENCE, 2 unordered
pieces
Length = 203316
Score = 79.4 bits (167), Expect(3) = 5e-034
Identities = 28/47 (59%), Positives = 38/47 (80%)
Frame = +1 / +2
Query: 178 DVATKGHGGLGVFVGVCIISAIFGTADANCQGALVGDLSLMCPEFIQ 318
D+AT G GGLG F+G+CI+S +FG ADA QG ++GD+SLM P+F+Q
Sbjct: 168848 DLATSGKGGLGTFIGICIVSGVFGIADALAQGGMIGDISLMHPDFMQ 168988
Score = 66.1 bits (138), Expect(3) = 5e-034
Identities = 30/48 (62%), Positives = 36/48 (75%)
Frame = +1 / +1
Query: 313 IQSFMAGLAASGVLTSALRLVTKAAFESSKDGLRIGAILFFSITCLFE 456
+QSF+AG AASG LTS LRL+TKA FE+SKDGLR GA F ++ E
Sbjct: 169069 LQSFLAGEAASGALTSVLRLITKAIFENSKDGLRKGASKFNTLMLSIE 169212
Score = 58.3 bits (121), Expect(3) = 1e-025
Identities = 24/38 (63%), Positives = 27/38 (71%)
Frame = -3 / -2
Query: 431 NSIAPMRRPSFELSKAALVTNLKADVRTPDAASPAMKD 318
N +AP+RRPS E S A V NLK DV P+AASPA KD
Sbjct: 169187 NLLAPLRRPSLEFSNIAFVINLKTDVSAPEAASPARKD 169074
Score = 55.6 bits (115), Expect(3) = 5e-034
Identities = 22/30 (73%), Positives = 25/30 (83%)
Frame = +1 / +2
Query: 37 VLTLVYQPFAFGITLIMTYYEAKMNTRLRN 126
VLTLVYQPFAFG I+ Y+EAK+NTR RN
Sbjct: 168614 VLTLVYQPFAFGTMAILAYHEAKLNTRKRN 168703
Score = 53.3 bits (110), Expect(3) = 1e-025
Identities = 25/46 (54%), Positives = 27/46 (58%)
Frame = -3 / -1
Query: 314 MNSGHIKERSPTSAP*QLASAVPNMALIMHTPTKTPSPPCPLVATS 177
M SG IK+ SP P ASA+P L MH P K PSPP P VA S
Sbjct: 168984 MKSGCIKDISPIIPPCARASAIPKTPLTMHIPMKVPSPPFPDVAKS 168847
Score = 42.8 bits (87), Expect(3) = 1e-025
Identities = 22/47 (46%), Positives = 27/47 (57%)
Frame = -3 / -1
Query: 176 RIINAKEPRKKRENPARFLNLVFIFAS*YVMMRVIPKAKGW*TSVST 36
+I A E +KK P RF LV FAS + ++PKA GW T VST
Sbjct: 168753 KINTAIELKKKSVYPDRFRFLVLSFASW*ASIAIVPKANGWYTRVST 168613
>gb|AC152156.30| Medicago truncatula clone mth2-136k22, WORKING DRAFT SEQUENCE, 7
unordered pieces
Length = 293363
Score = 79.4 bits (167), Expect(3) = 1e-033
Identities = 28/47 (59%), Positives = 38/47 (80%)
Frame = +1 / +2
Query: 178 DVATKGHGGLGVFVGVCIISAIFGTADANCQGALVGDLSLMCPEFIQ 318
D+AT G GGLG F+G+CI+S +FG ADA QG ++GD+SLM P+F+Q
Sbjct: 258896 DLATSGKGGLGTFIGICIVSGVFGIADALAQGGMIGDISLMHPDFMQ 259036
Score = 66.1 bits (138), Expect(3) = 1e-033
Identities = 30/48 (62%), Positives = 36/48 (75%)
Frame = +1 / +1
Query: 313 IQSFMAGLAASGVLTSALRLVTKAAFESSKDGLRIGAILFFSITCLFE 456
+QSF+AG AASG LTS LRL+TKA FE+SKDGLR GA F ++ E
Sbjct: 259117 LQSFLAGEAASGALTSVLRLITKAIFENSKDGLRKGASKFNTLMLSIE 259260
Score = 58.3 bits (121), Expect(3) = 1e-025
Identities = 24/38 (63%), Positives = 27/38 (71%)
Frame = -3 / -1
Query: 431 NSIAPMRRPSFELSKAALVTNLKADVRTPDAASPAMKD 318
N +AP+RRPS E S A V NLK DV P+AASPA KD
Sbjct: 259235 NLLAPLRRPSLEFSNIAFVINLKTDVSAPEAASPARKD 259122
Score = 55.6 bits (115), Expect(3) = 1e-033
Identities = 22/30 (73%), Positives = 25/30 (83%)
Frame = +1 / +2
Query: 37 VLTLVYQPFAFGITLIMTYYEAKMNTRLRN 126
VLTLVYQPFAFG I+ Y+EAK+NTR RN
Sbjct: 258662 VLTLVYQPFAFGTMAILAYHEAKLNTRKRN 258751
Score = 53.3 bits (110), Expect(3) = 1e-025
Identities = 25/46 (54%), Positives = 27/46 (58%)
Frame = -3 / -3
Query: 314 MNSGHIKERSPTSAP*QLASAVPNMALIMHTPTKTPSPPCPLVATS 177
M SG IK+ SP P ASA+P L MH P K PSPP P VA S
Sbjct: 259032 MKSGCIKDISPIIPPCARASAIPKTPLTMHIPMKVPSPPFPDVAKS 258895
Score = 42.8 bits (87), Expect(3) = 1e-025
Identities = 22/47 (46%), Positives = 27/47 (57%)
Frame = -3 / -3
Query: 176 RIINAKEPRKKRENPARFLNLVFIFAS*YVMMRVIPKAKGW*TSVST 36
+I A E +KK P RF LV FAS + ++PKA GW T VST
Sbjct: 258801 KINTAIELKKKSVYPDRFRFLVLSFASW*ASIAIVPKANGWYTRVST 258661
>gb|AC171168.2| Medicago truncatula chromosome 2 clone mth2-90o6, *** SEQUENCING IN
PROGRESS ***, 5 unordered pieces
Length = 140452
Score = 68.0 bits (142), Expect(4) = 5e-029
Identities = 29/40 (72%), Positives = 34/40 (85%)
Frame = +1 / +2
Query: 313 IQSFMAGLAASGVLTSALRLVTKAAFESSKDGLRIGAILF 432
+QSF+ GLAASG LTS LRL+TKAAF++SKDGLR GA F
Sbjct: 128102 LQSFLCGLAASGALTSGLRLITKAAFDNSKDGLRKGASKF 128221
Score = 57.0 bits (118), Expect(3) = 7e-021
Identities = 27/47 (57%), Positives = 30/47 (63%)
Frame = -3 / -2
Query: 317 *MNSGHIKERSPTSAP*QLASAVPNMALIMHTPTKTPSPPCPLVATS 177
*MNSG + ++SPT P* ASA P LI H P K PSPP P VA S
Sbjct: 127701 *MNSGFM*DKSPTIPP*T*ASATPKAPLIAHIPIKVPSPPLPDVAKS 127561
Score = 53.8 bits (111), Expect(3) = 7e-021
Identities = 23/38 (60%), Positives = 25/38 (65%)
Frame = -3 / -2
Query: 431 NSIAPMRRPSFELSKAALVTNLKADVRTPDAASPAMKD 318
N +AP RPS ELS AA V NLK +V PDAA P KD
Sbjct: 128220 NLLAPFLRPSLELSNAAFVINLKPEVNAPDAAKPHRKD 128107
Score = 53.3 bits (110), Expect(4) = 5e-029
Identities = 20/30 (66%), Positives = 26/30 (86%)
Frame = +1 / +2
Query: 421 AILFFSITCLFELVCLLLYTFVFGKLPIVK 510
A+LFF+I+ FEL+C+LLY FVF K+PIVK
Sbjct: 128402 AVLFFAISTFFELLCVLLYAFVFPKIPIVK 128491
Score = 53.3 bits (110), Expect(4) = 5e-029
Identities = 22/30 (73%), Positives = 25/30 (83%)
Frame = +1 / +3
Query: 37 VLTLVYQPFAFGITLIMTYYEAKMNTRLRN 126
VLTLVYQPFA G I++Y EAK+NTRLRN
Sbjct: 127341 VLTLVYQPFAVGTLAILSYNEAKVNTRLRN 127430
Score = 44.6 bits (91), Expect(3) = 7e-021
Identities = 22/38 (57%), Positives = 24/38 (63%)
Frame = -3 / -1
Query: 149 KKRENPARFLNLVFIFAS*YVMMRVIPKAKGW*TSVST 36
KKR P RFLNLV FAS + +P A GW TSVST
Sbjct: 127453 KKRVYPNRFLNLVLTFASL*DNIASVPTANGWYTSVST 127340
Score = 32.7 bits (65), Expect(4) = 5e-029
Identities = 15/37 (40%), Positives = 18/37 (48%)
Frame = +3 / +1
Query: 207 WCFRWCMHN*RHIWDS*C*LSRRTGWRPFFNVPRVHS 317
W F W M N* +W C SR G R + R+HS
Sbjct: 127591 WNFYWYMCN*WCLWSCRCSCSRWNGGRLVLHEARIHS 127701
>emb|CR338322.1| mte1-7D21RM1 BAC end, cultivar Jemalong A17 of Medicago truncatula,
genomic survey sequence
Length = 659
Score = 79.4 bits (167), Expect(2) = 6e-024
Identities = 31/47 (65%), Positives = 36/47 (76%)
Frame = +1 / +1
Query: 178 DVATKGHGGLGVFVGVCIISAIFGTADANCQGALVGDLSLMCPEFIQ 318
D+AT G GGLG F+G+C IS FG ADA+ QG +VGDLS M PEFIQ
Sbjct: 496 DLATSGKGGLGTFIGICAISGAFGVADAHVQGGMVGDLSYMKPEFIQ 636
Score = 53.3 bits (110), Expect(2) = 6e-024
Identities = 22/30 (73%), Positives = 25/30 (83%)
Frame = +1 / +2
Query: 37 VLTLVYQPFAFGITLIMTYYEAKMNTRLRN 126
VLTLVYQPFA G I++Y EAK+NTRLRN
Sbjct: 275 VLTLVYQPFAVGTLAILSYNEAKVNTRLRN 364
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 531,232,491
Number of Sequences: 392609
Number of extensions: 7701098
Number of successful extensions: 84824
Number of sequences better than 1.0e-020: 11
length of database: 147,244,331
effective HSP length: 53
effective length of database: 126,436,054
effective search space used: 14666582264
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)