TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCA10g02.yg.2.1
(277 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AC152887.20| Medicago truncatula clone mth2-91j4, WORKIN... 186 2e-046
gb|AC157648.18| Medicago truncatula clone mth2-68d9, comple... 186 2e-046
gb|AC144657.6| Medicago truncatula clone mth2-16a6, complet... 111 7e-037
gb|AC144540.6| Medicago truncatula clone mth2-19p16, comple... 111 7e-037
gb|AW690288.1|AW690288 NF031F12ST1F1000 Developing stem Med... 109 2e-036
gb|BQ137986.1|BQ137986 NF008A09PH1F1066 Phoma-infected Medi... 121 1e-029
>gb|AC152887.20| Medicago truncatula clone mth2-91j4, WORKING DRAFT SEQUENCE, 2 ordered
pieces
Length = 116277
Score = 186 bits (401), Expect = 2e-046
Identities = 72/91 (79%), Positives = 83/91 (91%)
Frame = -3 / -3
Query: 275 RVDLFTRQVSSPDVDWSYGEPTEMLCSGSNDGEGMGESAGAYIVRIPCGPRDKYLKKEAL 96
RVDLFTRQ+SSP+VDWSYGEPTEML +G++D + +GES+GAYI+RIP GPRDKYL KE L
Sbjct: 115975 RVDLFTRQISSPEVDWSYGEPTEMLTAGADDDDNIGESSGAYIIRIPFGPRDKYLPKELL 115796
Query: 95 WPYLQEFVDGALAHILNMSKALGEQVGNGRP 3
WPY+QEFVDGAL HILNMSKALGEQVG G+P
Sbjct: 115795 WPYVQEFVDGALTHILNMSKALGEQVGGGQP 115703
>gb|AC157648.18| Medicago truncatula clone mth2-68d9, complete sequence
Length = 84361
Score = 186 bits (401), Expect = 2e-046
Identities = 72/91 (79%), Positives = 83/91 (91%)
Frame = -3 / +1
Query: 275 RVDLFTRQVSSPDVDWSYGEPTEMLCSGSNDGEGMGESAGAYIVRIPCGPRDKYLKKEAL 96
RVDLFTRQ+SSP+VDWSYGEPTEML +G++D + +GES+GAYI+RIP GPRDKYL KE L
Sbjct: 57859 RVDLFTRQISSPEVDWSYGEPTEMLTAGADDDDNIGESSGAYIIRIPFGPRDKYLPKELL 58038
Query: 95 WPYLQEFVDGALAHILNMSKALGEQVGNGRP 3
WPY+QEFVDGAL HILNMSKALGEQVG G+P
Sbjct: 58039 WPYVQEFVDGALTHILNMSKALGEQVGGGQP 58131
>gb|AC144657.6| Medicago truncatula clone mth2-16a6, complete sequence
Length = 92738
Score = 111 bits (238), Expect(2) = 7e-037
Identities = 41/58 (70%), Positives = 51/58 (87%)
Frame = -3 / +3
Query: 182 GEGMGESAGAYIVRIPCGPRDKYLKKEALWPYLQEFVDGALAHILNMSKALGEQVGNG 9
G+ MGES+GAYI+RIP GPR+KY+ KE LWPY+ EFVDGA+ HI+ MSKALGEQ+G+G
Sbjct: 51849 GDDMGESSGAYIIRIPFGPRNKYIPKEELWPYIPEFVDGAIGHIIQMSKALGEQIGSG 52022
Score = 62.5 bits (130), Expect(2) = 7e-037
Identities = 25/34 (73%), Positives = 27/34 (79%)
Frame = -3 / +3
Query: 275 RVDLFTRQVSSPDVDWSYGEPTEMLCSGSNDGEG 174
RVDL TRQV+SPDVDWSYGEPTEML + D G
Sbjct: 51750 RVDLLTRQVASPDVDWSYGEPTEMLAPRNTDEFG 51851
>gb|AC144540.6| Medicago truncatula clone mth2-19p16, complete sequence
Length = 78738
Score = 111 bits (238), Expect(2) = 7e-037
Identities = 41/58 (70%), Positives = 51/58 (87%)
Frame = -3 / -3
Query: 182 GEGMGESAGAYIVRIPCGPRDKYLKKEALWPYLQEFVDGALAHILNMSKALGEQVGNG 9
G+ MGES+GAYI+RIP GPR+KY+ KE LWPY+ EFVDGA+ HI+ MSKALGEQ+G+G
Sbjct: 58993 GDDMGESSGAYIIRIPFGPRNKYIPKEELWPYIPEFVDGAIGHIIQMSKALGEQIGSG 58820
Score = 62.5 bits (130), Expect(2) = 7e-037
Identities = 25/34 (73%), Positives = 27/34 (79%)
Frame = -3 / -3
Query: 275 RVDLFTRQVSSPDVDWSYGEPTEMLCSGSNDGEG 174
RVDL TRQV+SPDVDWSYGEPTEML + D G
Sbjct: 59092 RVDLLTRQVASPDVDWSYGEPTEMLAPRNTDEFG 58991
>gb|AW690288.1|AW690288 NF031F12ST1F1000 Developing stem Medicago truncatula cDNA clone
NF031F12ST 5', mRNA sequence
Length = 648
Score = 109 bits (232), Expect(2) = 2e-036
Identities = 40/57 (70%), Positives = 50/57 (87%)
Frame = -3 / +3
Query: 182 GEGMGESAGAYIVRIPCGPRDKYLKKEALWPYLQEFVDGALAHILNMSKALGEQVGN 12
G+ MGES+GAYI+RIP GPR+KY+ KE LWPY+ EFVDGA+ HI+ MSKALGEQ+G+
Sbjct: 408 GDDMGESSGAYIIRIPFGPRNKYIXKEELWPYIPEFVDGAIGHIIQMSKALGEQIGS 578
Score = 62.5 bits (130), Expect(2) = 2e-036
Identities = 25/34 (73%), Positives = 27/34 (79%)
Frame = -3 / +3
Query: 275 RVDLFTRQVSSPDVDWSYGEPTEMLCSGSNDGEG 174
RVDL TRQV+SPDVDWSYGEPTEML + D G
Sbjct: 309 RVDLLTRQVASPDVDWSYGEPTEMLAPRNTDEFG 410
>gb|BQ137986.1|BQ137986 NF008A09PH1F1066 Phoma-infected Medicago truncatula cDNA clone
NF008A09PH 5', mRNA sequence
Length = 658
Score = 121 bits (258), Expect(2) = 1e-029
Identities = 47/63 (74%), Positives = 55/63 (87%)
Frame = -3 / +3
Query: 191 SNDGEGMGESAGAYIVRIPCGPRDKYLKKEALWPYLQEFVDGALAHILNMSKALGEQVGN 12
++D GES+GAYI+RIP GPRDKYL+KE LWP++QEFVDGALAHILNMSK LGEQVG
Sbjct: 192 NDDDGSTGESSGAYIIRIPFGPRDKYLEKELLWPHIQEFVDGALAHILNMSKVLGEQVGG 371
Query: 11 GRP 3
G+P
Sbjct: 372 GQP 380
Score = 71.2 bits (149), Expect(2) = 7e-023
Identities = 26/38 (68%), Positives = 31/38 (81%)
Frame = -3 / +3
Query: 275 RVDLFTRQVSSPDVDWSYGEPTEMLCSGSNDGEGMGES 162
RVDLFTRQ+SSPD+DWSYGEPTEML +G D + G +
Sbjct: 99 RVDLFTRQISSPDIDWSYGEPTEMLSAGQEDNDDDGST 212
Score = 54.2 bits (112), Expect(2) = 7e-023
Identities = 20/52 (38%), Positives = 34/52 (65%)
Frame = -2 / +2
Query: 165 ECRSLHCAHTVWTTG*IPQEGSTVALPPRVCRWSSCAYP*HVQGSGRTGWKW 10
E R ++ +T+W+T *I +E +++A R+CRWS ++ HV+ G T W+W
Sbjct: 218 EQRCIYHKNTLWSTR*ISRERTSLASHSRICRWSLSSHSQHVKSIG*TSWRW 373
Score = 27.6 bits (54), Expect(2) = 1e-029
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -1 / +1
Query: 265 SSLVKCHLLTWTGAMVSRRRCYAPV 191
SS K HLL GAM + ++CY V
Sbjct: 109 SSRAKYHLLILIGAMENLQKCYLQV 183
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 277,137,235
Number of Sequences: 392609
Number of extensions: 3270333
Number of successful extensions: 161065
Number of sequences better than 1.0e-020: 8
length of database: 147,244,331
effective HSP length: 50
effective length of database: 127,613,881
effective search space used: 5232169121
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)