TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBTB.006E19F020311.3.1
(789 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AC146548.9| Medicago truncatula clone mth2-2p3, complete... 113 2e-041
gb|AC146329.18| Medicago truncatula clone mth2-5i21, comple... 113 2e-041
gb|AC171394.2| Medicago truncatula clone mth2-38p7, WORKING... 113 5e-041
gb|BE203027.1|BE203027 EST403049 KV1 Medicago truncatula cD... 147 1e-038
>gb|AC146548.9| Medicago truncatula clone mth2-2p3, complete sequence
Length = 129327
Score = 113 bits (242), Expect(3) = 6e-036
Identities = 45/57 (78%), Positives = 52/57 (91%)
Frame = +2 / -2
Query: 479 VMLTYLVYEKQQKLRIMMKMHGLKDGPYWLISYSYFLALSIVYMLFFMIFGSLIGLN 649
V+LT LVYEKQQKLRIMMKMHGL DGPYWLISY YFLALS++Y+L F+IFGS++G N
Sbjct: 118442 VILTSLVYEKQQKLRIMMKMHGLGDGPYWLISYGYFLALSVIYILCFVIFGSVLGKN 118272
Score = 112 bits (240), Expect(4) = 2e-041
Identities = 43/57 (75%), Positives = 52/57 (91%)
Frame = +2 / -3
Query: 479 VMLTYLVYEKQQKLRIMMKMHGLKDGPYWLISYSYFLALSIVYMLFFMIFGSLIGLN 649
V+LT L+YEKQQKLRIMMKMHGL DGPYW+I+Y YFLALS++YML F+IFGS++G N
Sbjct: 83128 VVLTSLIYEKQQKLRIMMKMHGLGDGPYWMITYGYFLALSVIYMLCFVIFGSVLGKN 82958
Score = 109 bits (233), Expect(4) = 9e-031
Identities = 44/57 (77%), Positives = 50/57 (87%)
Frame = +2 / -2
Query: 479 VMLTYLVYEKQQKLRIMMKMHGLKDGPYWLISYSYFLALSIVYMLFFMIFGSLIGLN 649
V+LT LVYEKQQKLRIMMKMHGL DGPYWLI+Y YFLALS +YML F++FGS +G N
Sbjct: 100652 VILTSLVYEKQQKLRIMMKMHGLGDGPYWLITYGYFLALSAIYMLCFVMFGSSLGKN 100482
Score = 56.5 bits (117), Expect(3) = 6e-036
Identities = 23/54 (42%), Positives = 30/54 (55%)
Frame = +2 / -2
Query: 320 VSNAYLKLIKGTGVDMLLEFVKDMPKVGTXXXXXXXXXXXXXXXTWIIELLFPV 481
+SNAYL+ ++G G +L EFVKDMPK T TW++ LFPV
Sbjct: 118901 ISNAYLQFLRGPGTKILFEFVKDMPKPETVFRIELASLLGGLFFTWVVLQLFPV 118740
Score = 56.1 bits (116), Expect(4) = 2e-041
Identities = 23/54 (42%), Positives = 30/54 (55%)
Frame = +2 / -1
Query: 320 VSNAYLKLIKGTGVDMLLEFVKDMPKVGTXXXXXXXXXXXXXXXTWIIELLFPV 481
+SNAYL+ ++G G ML EFVK+MPK T TW++ LFPV
Sbjct: 83634 ISNAYLQFLRGLGTKMLFEFVKEMPKSETPLRIEIASLLGVLFFTWVVLQLFPV 83473
Score = 42.8 bits (87), Expect(4) = 2e-041
Identities = 16/45 (35%), Positives = 24/45 (53%)
Frame = +2 / -2
Query: 188 IAGYDFLGTTQYGLGVNVWYNSTYNDNNAYSFISTLRVPRLVNAV 322
+ +DFL + + G V VWY ST + + LR+PR +N V
Sbjct: 84296 LTAFDFLNSNENGFNVTVWYKSTNKGVTNFGPTALLRIPRSINLV 84162
Score = 41.8 bits (85), Expect(4) = 9e-031
Identities = 15/29 (51%), Positives = 22/29 (75%)
Frame = +2 / -2
Query: 320 VSNAYLKLIKGTGVDMLLEFVKDMPKVGT 406
+SNAYL+ ++G G +L EFVK++PK T
Sbjct: 101093 ISNAYLQFLRGPGTKILFEFVKELPKHDT 101007
Score = 40.9 bits (83), Expect(4) = 2e-041
Identities = 15/30 (50%), Positives = 21/30 (70%)
Frame = +2 / -2
Query: 65 IPFQLNIQCVEGAPLWRETASIINHEFLKG 154
IPF + I+C +G LWR ++S IN+E KG
Sbjct: 120497 IPFDVEIRCGQGINLWRNSSSDINNELYKG 120408
Score = 38.6 bits (78), Expect(3) = 6e-036
Identities = 15/45 (33%), Positives = 24/45 (53%)
Frame = +2 / -2
Query: 188 IAGYDFLGTTQYGLGVNVWYNSTYNDNNAYSFISTLRVPRLVNAV 322
+ +DFL + + G + VWY S ++ + LR+PR VN V
Sbjct: 120317 LTAFDFLNSNEDGFDIVVWYKSVFSRGIHSPSTTLLRIPRSVNLV 120183
Score = 32.7 bits (65), Expect(4) = 9e-031
Identities = 9/24 (37%), Positives = 16/24 (66%)
Frame = +2 / -2
Query: 185 FIAGYDFLGTTQYGLGVNVWYNST 256
++ +DFL + + G + +WYNST
Sbjct: 109577 YLTAFDFLNSNEDGFNIALWYNST 109506
Score = 32.2 bits (64), Expect(4) = 9e-031
Identities = 12/28 (42%), Positives = 17/28 (60%)
Frame = +2 / -2
Query: 68 PFQLNIQCVEGAPLWRETASIINHEFLK 151
PF + I C + LWR ++S IN+E K
Sbjct: 109784 PFDIGISCGQVINLWRNSSSDINNELYK 109701
>gb|AC146329.18| Medicago truncatula clone mth2-5i21, complete sequence
Length = 129327
Score = 113 bits (242), Expect(3) = 6e-036
Identities = 45/57 (78%), Positives = 52/57 (91%)
Frame = +2 / -2
Query: 479 VMLTYLVYEKQQKLRIMMKMHGLKDGPYWLISYSYFLALSIVYMLFFMIFGSLIGLN 649
V+LT LVYEKQQKLRIMMKMHGL DGPYWLISY YFLALS++Y+L F+IFGS++G N
Sbjct: 118442 VILTSLVYEKQQKLRIMMKMHGLGDGPYWLISYGYFLALSVIYILCFVIFGSVLGKN 118272
Score = 112 bits (240), Expect(4) = 2e-041
Identities = 43/57 (75%), Positives = 52/57 (91%)
Frame = +2 / -3
Query: 479 VMLTYLVYEKQQKLRIMMKMHGLKDGPYWLISYSYFLALSIVYMLFFMIFGSLIGLN 649
V+LT L+YEKQQKLRIMMKMHGL DGPYW+I+Y YFLALS++YML F+IFGS++G N
Sbjct: 83128 VVLTSLIYEKQQKLRIMMKMHGLGDGPYWMITYGYFLALSVIYMLCFVIFGSVLGKN 82958
Score = 109 bits (233), Expect(4) = 9e-031
Identities = 44/57 (77%), Positives = 50/57 (87%)
Frame = +2 / -2
Query: 479 VMLTYLVYEKQQKLRIMMKMHGLKDGPYWLISYSYFLALSIVYMLFFMIFGSLIGLN 649
V+LT LVYEKQQKLRIMMKMHGL DGPYWLI+Y YFLALS +YML F++FGS +G N
Sbjct: 100652 VILTSLVYEKQQKLRIMMKMHGLGDGPYWLITYGYFLALSAIYMLCFVMFGSSLGKN 100482
Score = 56.5 bits (117), Expect(3) = 6e-036
Identities = 23/54 (42%), Positives = 30/54 (55%)
Frame = +2 / -2
Query: 320 VSNAYLKLIKGTGVDMLLEFVKDMPKVGTXXXXXXXXXXXXXXXTWIIELLFPV 481
+SNAYL+ ++G G +L EFVKDMPK T TW++ LFPV
Sbjct: 118901 ISNAYLQFLRGPGTKILFEFVKDMPKPETVFRIELASLLGGLFFTWVVLQLFPV 118740
Score = 56.1 bits (116), Expect(4) = 2e-041
Identities = 23/54 (42%), Positives = 30/54 (55%)
Frame = +2 / -1
Query: 320 VSNAYLKLIKGTGVDMLLEFVKDMPKVGTXXXXXXXXXXXXXXXTWIIELLFPV 481
+SNAYL+ ++G G ML EFVK+MPK T TW++ LFPV
Sbjct: 83634 ISNAYLQFLRGLGTKMLFEFVKEMPKSETPLRIEIASLLGVLFFTWVVLQLFPV 83473
Score = 42.8 bits (87), Expect(4) = 2e-041
Identities = 16/45 (35%), Positives = 24/45 (53%)
Frame = +2 / -2
Query: 188 IAGYDFLGTTQYGLGVNVWYNSTYNDNNAYSFISTLRVPRLVNAV 322
+ +DFL + + G V VWY ST + + LR+PR +N V
Sbjct: 84296 LTAFDFLNSNENGFNVTVWYKSTNKGVTNFGPTALLRIPRSINLV 84162
Score = 41.8 bits (85), Expect(4) = 9e-031
Identities = 15/29 (51%), Positives = 22/29 (75%)
Frame = +2 / -2
Query: 320 VSNAYLKLIKGTGVDMLLEFVKDMPKVGT 406
+SNAYL+ ++G G +L EFVK++PK T
Sbjct: 101093 ISNAYLQFLRGPGTKILFEFVKELPKHDT 101007
Score = 40.9 bits (83), Expect(4) = 2e-041
Identities = 15/30 (50%), Positives = 21/30 (70%)
Frame = +2 / -2
Query: 65 IPFQLNIQCVEGAPLWRETASIINHEFLKG 154
IPF + I+C +G LWR ++S IN+E KG
Sbjct: 120497 IPFDVEIRCGQGINLWRNSSSDINNELYKG 120408
Score = 38.6 bits (78), Expect(3) = 6e-036
Identities = 15/45 (33%), Positives = 24/45 (53%)
Frame = +2 / -2
Query: 188 IAGYDFLGTTQYGLGVNVWYNSTYNDNNAYSFISTLRVPRLVNAV 322
+ +DFL + + G + VWY S ++ + LR+PR VN V
Sbjct: 120317 LTAFDFLNSNEDGFDIVVWYKSVFSRGIHSPSTTLLRIPRSVNLV 120183
Score = 32.7 bits (65), Expect(4) = 9e-031
Identities = 9/24 (37%), Positives = 16/24 (66%)
Frame = +2 / -2
Query: 185 FIAGYDFLGTTQYGLGVNVWYNST 256
++ +DFL + + G + +WYNST
Sbjct: 109577 YLTAFDFLNSNEDGFNIALWYNST 109506
Score = 32.2 bits (64), Expect(4) = 9e-031
Identities = 12/28 (42%), Positives = 17/28 (60%)
Frame = +2 / -2
Query: 68 PFQLNIQCVEGAPLWRETASIINHEFLK 151
PF + I C + LWR ++S IN+E K
Sbjct: 109784 PFDIGISCGQVINLWRNSSSDINNELYK 109701
>gb|AC171394.2| Medicago truncatula clone mth2-38p7, WORKING DRAFT SEQUENCE, 2
unordered pieces
Length = 91342
Score = 113 bits (242), Expect(4) = 5e-041
Identities = 45/57 (78%), Positives = 52/57 (91%)
Frame = +2 / -3
Query: 479 VMLTYLVYEKQQKLRIMMKMHGLKDGPYWLISYSYFLALSIVYMLFFMIFGSLIGLN 649
V+LT LVYEKQQKLRIMMKMHGL DGPYWLISY YFLALS++Y+L F+IFGS++G N
Sbjct: 4316 VILTSLVYEKQQKLRIMMKMHGLGDGPYWLISYGYFLALSVIYILCFVIFGSVLGKN 4146
Score = 56.5 bits (117), Expect(4) = 5e-041
Identities = 23/54 (42%), Positives = 30/54 (55%)
Frame = +2 / -3
Query: 320 VSNAYLKLIKGTGVDMLLEFVKDMPKVGTXXXXXXXXXXXXXXXTWIIELLFPV 481
+SNAYL+ ++G G +L EFVKDMPK T TW++ LFPV
Sbjct: 4775 ISNAYLQFLRGPGTKILFEFVKDMPKPETVFRIELASLLGGLFFTWVVLQLFPV 4614
Score = 40.9 bits (83), Expect(4) = 5e-041
Identities = 15/30 (50%), Positives = 21/30 (70%)
Frame = +2 / -3
Query: 65 IPFQLNIQCVEGAPLWRETASIINHEFLKG 154
IPF + I+C +G LWR ++S IN+E KG
Sbjct: 6371 IPFDVEIRCGQGINLWRNSSSDINNELYKG 6282
Score = 38.6 bits (78), Expect(4) = 5e-041
Identities = 15/45 (33%), Positives = 24/45 (53%)
Frame = +2 / -3
Query: 188 IAGYDFLGTTQYGLGVNVWYNSTYNDNNAYSFISTLRVPRLVNAV 322
+ +DFL + + G + VWY S ++ + LR+PR VN V
Sbjct: 6191 LTAFDFLNSNEDGFDIVVWYKSVFSRGIHSPSTTLLRIPRSVNLV 6057
>gb|BE203027.1|BE203027 EST403049 KV1 Medicago truncatula cDNA clone pKV1-4E9, mRNA
sequence
Length = 529
Score = 147 bits (315), Expect(2) = 1e-038
Identities = 63/122 (51%), Positives = 78/122 (63%)
Frame = +2 / +2
Query: 173 QINEFIAGYDFLGTTQYGLGVNVWYNSTYNDNNAYSFISTLRVPRLVNAVSNAYLKLIKG 352
QINE AGYDFL + + V++WYNSTY ++ + I+ R+PR VN VSN+YL+ + G
Sbjct: 164 QINEITAGYDFLNSNEDTFNVSIWYNSTYKNDTGFDKIALARIPRSVNLVSNSYLQFLLG 343
Query: 353 TGVDMLLEFVKDMPKVGTXXXXXXXXXXXXXXXTWIIELLFPVMLTYLVYEKQQKLRIMM 532
+G ML EFVK+MPK T TW+I LFPV LT LVYEKQQ LRIMM
Sbjct: 344 SGTKMLFEFVKEMPKPETPLKFDVASLLGGLFFTWVILQLFPVGLTSLVYEKQQNLRIMM 523
Query: 533 KM 538
KM
Sbjct: 524 KM 529
Score = 34.5 bits (69), Expect(2) = 1e-038
Identities = 11/30 (36%), Positives = 19/30 (63%)
Frame = +2 / +2
Query: 74 QLNIQCVEGAPLWRETASIINHEFLKGYRQ 163
Q ++C + LWR ++S +N+ KGYR+
Sbjct: 59 QQELRCAQALRLWRNSSSEVNNAIYKGYRK 148
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 862,938,527
Number of Sequences: 392609
Number of extensions: 12926514
Number of successful extensions: 64704
Number of sequences better than 1.0e-020: 5
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 26217036560
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)