TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBL17f07.xg.2.1
(657 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BE202402.1|BE202402 EST392851 KV1 Medicago truncatula cD... 72 4e-026
gb|AJ846141.1|AJ846141 AJ846141 MtSC4 Medicago truncatula c... 71 1e-025
gb|AC151458.24| Medicago truncatula clone mth2-105a19, WORK... 76 6e-024
emb|CR335423.1| mte1-66I1FM1 BAC end, cultivar Jemalong A17... 76 6e-024
emb|CR308614.1| mte1-3M15FM1 BAC end, cultivar Jemalong A17... 76 6e-024
gb|CA921476.1|CA921476 EST639194 MTUS Medicago truncatula c... 71 8e-023
gb|CF069243.1|CF069243 EST669964 MTUS Medicago truncatula c... 74 4e-022
gb|BE202392.1|BE202392 EST392841 KV1 Medicago truncatula cD... 69 5e-022
>gb|BE202402.1|BE202402 EST392851 KV1 Medicago truncatula cDNA clone pKV1-2G17, mRNA
sequence
Length = 542
Score = 71.6 bits (150), Expect(2) = 4e-026
Identities = 27/76 (35%), Positives = 48/76 (63%)
Frame = +3 / +1
Query: 72 MDIRRDFHMAEGEGEWSYSKNCRRQQVAVXXTRPMVETAVKQVYAALLPRTMVVADLGCS 251
M + + HM GEG+ SY+ N Q++ + + ++E ++ ++Y P + VADLGCS
Sbjct: 34 MAVEQVLHMNGGEGDTSYANNSTFQRMVMLTAKHILEESIMRLYCDTFPNCLKVADLGCS 213
Query: 252 AGPNTLLFISSVLSSI 299
+GPN LL S+++++I
Sbjct: 214 SGPNALLVASNIINTI 261
Score = 67.5 bits (141), Expect(2) = 4e-026
Identities = 29/73 (39%), Positives = 39/73 (53%)
Frame = +3 / +1
Query: 372 QFVLNDLPGNDFNHLFRSVEEEFRRAAGCERAPHPPYYVMGLPESYYNRLFPRQSVHLFX 551
QF LNDL GNDFN F+ + + +R + P + G P S+Y RLFP S+H F
Sbjct: 304 QFFLNDLFGNDFNTTFKLLPDFIKRLQEEKGQKFSPCFFSGTPGSFYGRLFPDNSIHFFH 483
Query: 552 SSYXXQXRXQEPE 590
SSY + P+
Sbjct: 484 SSYSLHWLSKTPD 522
>gb|AJ846141.1|AJ846141 AJ846141 MtSC4 Medicago truncatula cDNA clone MtC407C05N2, mRNA
sequence
Length = 687
Score = 71.2 bits (149), Expect(2) = 1e-025
Identities = 33/90 (36%), Positives = 44/90 (48%)
Frame = +3 / +3
Query: 372 QFVLNDLPGNDFNHLFRSVEEEFRRAAGCERAPHPPYYVMGLPESYYNRLFPRQSVHLFX 551
QF LNDL GNDFN F+ + + +R + P + G P S+Y RLFP S+H F
Sbjct: 267 QFFLNDLFGNDFNTTFKLLPDFIKRLQEEKGQKFSPCFFSGTPGSFYGRLFPDNSIHFFH 446
Query: 552 SSYXXQXRXQEPEGXXAXRKPCLNXXXIYI 641
SSY + P+ LN IY+
Sbjct: 447 SSYSLHWLSKTPDALQDAAIEPLNKGNIYL 536
Score = 66.1 bits (138), Expect(2) = 1e-025
Identities = 25/68 (36%), Positives = 44/68 (64%)
Frame = +3 / +3
Query: 96 MAEGEGEWSYSKNCRRQQVAVXXTRPMVETAVKQVYAALLPRTMVVADLGCSAGPNTLLF 275
M GEG+ SY+ N Q++ + + ++E ++ ++Y P + VADLGCS+GPN LL
Sbjct: 21 MNGGEGDTSYANNSTFQRMVMLTAKHILEESIMRLYCDTFPNCLKVADLGCSSGPNALLV 200
Query: 276 ISSVLSSI 299
S+++++I
Sbjct: 201 ASNIINTI 224
>gb|AC151458.24| Medicago truncatula clone mth2-105a19, WORKING DRAFT SEQUENCE
Length = 109595
Score = 75.8 bits (159), Expect(2) = 6e-024
Identities = 30/65 (46%), Positives = 41/65 (63%)
Frame = +3 / +1
Query: 366 ELQFVLNDLPGNDFNHLFRSVEEEFRRAAGCERAPHPPYYVMGLPESYYNRLFPRQSVHL 545
E + LNDLPGNDFN++FRS++ +R G Y+ G+P S+Y R+FP QS+H
Sbjct: 22906 EYKVFLNDLPGNDFNNIFRSLDNFKKRLHGETETEMDQCYISGVPGSFYGRIFPNQSLHF 23085
Query: 546 FXSSY 560
SSY
Sbjct: 23086 VHSSY 23100
Score = 56.1 bits (116), Expect(2) = 6e-024
Identities = 20/52 (38%), Positives = 33/52 (63%)
Frame = +3 / +1
Query: 144 QQVAVXXTRPMVETAVKQVYAALLPRTMVVADLGCSAGPNTLLFISSVLSSI 299
QQ + T+ + E A+ +Y + P ++ +ADLGCS+GPNTLL +S + +
Sbjct: 22714 QQQVLSLTKSIREEAITSLYCSAYPNSLAIADLGCSSGPNTLLVVSEFIKVV 22869
>emb|CR335423.1| mte1-66I1FM1 BAC end, cultivar Jemalong A17 of Medicago truncatula,
genomic survey sequence
Length = 730
Score = 75.8 bits (159), Expect(2) = 6e-024
Identities = 30/65 (46%), Positives = 41/65 (63%)
Frame = +3 / +1
Query: 366 ELQFVLNDLPGNDFNHLFRSVEEEFRRAAGCERAPHPPYYVMGLPESYYNRLFPRQSVHL 545
E + LNDLPGNDFN++FRS++ +R G Y+ G+P S+Y R+FP QS+H
Sbjct: 274 EYKVFLNDLPGNDFNNIFRSLDNFKKRLHGETETEMDQCYISGVPGSFYGRIFPNQSLHF 453
Query: 546 FXSSY 560
SSY
Sbjct: 454 VHSSY 468
Score = 56.1 bits (116), Expect(2) = 6e-024
Identities = 20/52 (38%), Positives = 33/52 (63%)
Frame = +3 / +1
Query: 144 QQVAVXXTRPMVETAVKQVYAALLPRTMVVADLGCSAGPNTLLFISSVLSSI 299
QQ + T+ + E A+ +Y + P ++ +ADLGCS+GPNTLL +S + +
Sbjct: 82 QQQVLSLTKSIREEAITSLYCSAYPNSLAIADLGCSSGPNTLLVVSEFIKVV 237
>emb|CR308614.1| mte1-3M15FM1 BAC end, cultivar Jemalong A17 of Medicago truncatula,
genomic survey sequence
Length = 728
Score = 75.8 bits (159), Expect(2) = 6e-024
Identities = 30/65 (46%), Positives = 41/65 (63%)
Frame = +3 / +3
Query: 366 ELQFVLNDLPGNDFNHLFRSVEEEFRRAAGCERAPHPPYYVMGLPESYYNRLFPRQSVHL 545
E + LNDLPGNDFN++FRS++ +R G Y+ G+P S+Y R+FP QS+H
Sbjct: 276 EYKVFLNDLPGNDFNNIFRSLDNFKKRLHGETETEMDQCYISGVPGSFYGRIFPNQSLHF 455
Query: 546 FXSSY 560
SSY
Sbjct: 456 VHSSY 470
Score = 56.1 bits (116), Expect(2) = 6e-024
Identities = 20/52 (38%), Positives = 33/52 (63%)
Frame = +3 / +3
Query: 144 QQVAVXXTRPMVETAVKQVYAALLPRTMVVADLGCSAGPNTLLFISSVLSSI 299
QQ + T+ + E A+ +Y + P ++ +ADLGCS+GPNTLL +S + +
Sbjct: 84 QQQVLSLTKSIREEAITSLYCSAYPNSLAIADLGCSSGPNTLLVVSEFIKVV 239
>gb|CA921476.1|CA921476 EST639194 MTUS Medicago truncatula cDNA clone MTUS-40G6, mRNA
sequence
Length = 640
Score = 71.2 bits (149), Expect(3) = 8e-023
Identities = 33/90 (36%), Positives = 44/90 (48%)
Frame = +3 / +2
Query: 372 QFVLNDLPGNDFNHLFRSVEEEFRRAAGCERAPHPPYYVMGLPESYYNRLFPRQSVHLFX 551
QF LNDL GNDFN F+ + + +R + P + G P S+Y RLFP S+H F
Sbjct: 305 QFFLNDLFGNDFNTTFKLLPDFIKRLQEEKGQKFSPCFFSGTPGSFYGRLFPDNSIHFFH 484
Query: 552 SSYXXQXRXQEPEGXXAXRKPCLNXXXIYI 641
SSY + P+ LN IY+
Sbjct: 485 SSYSLHWLSKTPDALQDAAIEPLNKGNIYL 574
Score = 47.8 bits (98), Expect(3) = 8e-023
Identities = 17/40 (42%), Positives = 28/40 (70%)
Frame = +3 / +2
Query: 180 ETAVKQVYAALLPRTMVVADLGCSAGPNTLLFISSVLSSI 299
E ++ ++Y P + VADLGCS+GPN LL S+++++I
Sbjct: 143 EESIMRLYCDTFPNCLKVADLGCSSGPNALLVASNIINTI 262
Score = 26.7 bits (52), Expect(3) = 8e-023
Identities = 10/33 (30%), Positives = 18/33 (54%)
Frame = +3 / +1
Query: 72 MDIRRDFHMAEGEGEWSYSKNCRRQQVAVXXTR 170
M + + HM GEG+ SY+ N Q++ + +
Sbjct: 34 MAVEQVLHMNGGEGDTSYANNSTFQRMVMLTAK 132
>gb|CF069243.1|CF069243 EST669964 MTUS Medicago truncatula cDNA clone MTUS-18A11, mRNA
sequence
Length = 502
Score = 74.4 bits (156), Expect(3) = 4e-022
Identities = 29/76 (38%), Positives = 47/76 (61%)
Frame = +3 / +1
Query: 72 MDIRRDFHMAEGEGEWSYSKNCRRQQVAVXXTRPMVETAVKQVYAALLPRTMVVADLGCS 251
MD+ + FHM G G+ SY+KN Q+ A + ++ V+++Y P+++ +ADLGCS
Sbjct: 7 MDVEKVFHMTGGVGKTSYAKNSSLQKKASDKVKHIIIETVEELYIETTPKSIGIADLGCS 186
Query: 252 AGPNTLLFISSVLSSI 299
+GPNTL I + +I
Sbjct: 187 SGPNTLSIIKDIFQTI 234
Score = 35.4 bits (71), Expect(3) = 4e-022
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +3 / +1
Query: 357 HHVELQFVLNDLPGNDFNHLFRSVEE 434
H E + NDLP NDFN +F+++ E
Sbjct: 262 HSTEFRVYFNDLPTNDFNSIFKALPE 339
Score = 33.6 bits (67), Expect(3) = 4e-022
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +3 / +1
Query: 474 PPYYVMGLPESYYNRLFPRQSVHLFXSSY 560
P ++ G P S+Y RLFP +H SS+
Sbjct: 379 PSIFMGGYPGSFYGRLFPNSYLHFVHSSH 465
>gb|BE202392.1|BE202392 EST392841 KV1 Medicago truncatula cDNA clone pKV1-2G5, mRNA
sequence
Length = 474
Score = 68.9 bits (144), Expect(2) = 5e-022
Identities = 26/76 (34%), Positives = 47/76 (61%)
Frame = +3 / +1
Query: 72 MDIRRDFHMAEGEGEWSYSKNCRRQQVAVXXTRPMVETAVKQVYAALLPRTMVVADLGCS 251
M + + HM GEG+ SY+ N Q++ + + ++E ++ ++Y P + VADLGCS
Sbjct: 34 MAVEQVLHMNGGEGDTSYANNSTFQRMVMLTAKHILEESIMRLYCDTFPNCLKVADLGCS 213
Query: 252 AGPNTLLFISSVLSSI 299
+ PN LL S+++++I
Sbjct: 214 SSPNALLVASNIINTI 261
Score = 56.5 bits (117), Expect(2) = 5e-022
Identities = 24/57 (42%), Positives = 32/57 (56%)
Frame = +3 / +1
Query: 372 QFVLNDLPGNDFNHLFRSVEEEFRRAAGCERAPHPPYYVMGLPESYYNRLFPRQSVH 542
QF LNDL GNDFN F+ + + +R + P + G P S+Y RLFP S+H
Sbjct: 304 QFFLNDLFGNDFNTTFKLLPDFIKRLQEEKGQKFSPCFFSGTPGSFYGRLFPDNSIH 474
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 511,249,191
Number of Sequences: 392609
Number of extensions: 5691709
Number of successful extensions: 31739
Number of sequences better than 1.0e-020: 8
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 20671124980
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)