TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAZ2b01.yg.2.1
(541 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ255277.1|BQ255277 MTNAE06TKM KVKC Medicago truncatula ... 173 5e-045
gb|AW299129.1|AW299129 EST305803 KV2 Medicago truncatula cD... 170 1e-041
gb|CX532373.1|CX532373 s13dNF61A03MJ017_271039 Methyl Jasmo... 149 7e-036
gb|AW191220.1|AW191220 T113394e KV2 Medicago truncatula cDN... 133 8e-034
gb|AC147538.16| Medicago truncatula clone mth2-135c7, WORKI... 79 3e-032
gb|AC147538.15| Medicago truncatula clone mth2-135c7, WORKI... 79 3e-032
gb|BI308772.1|BI308772 EST530182 GPOD Medicago truncatula c... 114 1e-024
gb|AC170800.5| Medicago truncatula clone mth2-32n7, complet... 90 3e-021
gb|AC141922.19| Medicago truncatula clone mth2-9n11, comple... 90 4e-021
gb|AC144931.28| Medicago truncatula clone mth2-22b18, compl... 90 5e-021
gb|AC142094.12| Medicago truncatula clone mth2-34h6, comple... 90 7e-021
>gb|BQ255277.1|BQ255277 MTNAE06TKM KVKC Medicago truncatula cDNA clone pKVKC-5A6, mRNA
sequence
Length = 795
Score = 173 bits (372), Expect(2) = 5e-045
Identities = 73/95 (76%), Positives = 85/95 (89%)
Frame = +3 / +3
Query: 12 LXNDVLDRAKIEAGKLELEAVPFDLRSLMDDVISLFSSKSREKCIELAVFVCDNVPKDVL 191
L N+VLDRAKIEAGKLELEAVPFDLRS++DDV+SLFS KSR K +ELAVFV D VP V+
Sbjct: 237 LINEVLDRAKIEAGKLELEAVPFDLRSILDDVLSLFSEKSRHKGLELAVFVSDKVPDIVM 416
Query: 192 GDPWRFRQILTNLVGNAVKFTERGHVFVRVCLADN 296
GDP RFRQI+TNLVGN+VKFTERGH+FV+V L++N
Sbjct: 417 GDPGRFRQIVTNLVGNSVKFTERGHIFVKVHLSEN 521
Score = 138 bits (295), Expect = 9e-032
Identities = 66/97 (68%), Positives = 69/97 (71%)
Frame = -1 / -3
Query: 301 FELSAKQTRTNT*PRSVNLTALPTKFVSICRNLQGSPRTSFGTLSHTNTASSMHFSRDFE 122
F S K T TN PRSVNLT LPT+FV+IC NL GSP T GTLS TNTASS DF
Sbjct: 526 FLFSDK*TLTNIWPRSVNLTELPTRFVTICLNLPGSPITISGTLSETNTASSKPLCLDFS 347
Query: 121 ENKEITSSMRERRSKGTASSSNFPASIFARSRTSFXN 11
E +E TSS ERRS GTASSSN PASIFARS TSF N
Sbjct: 346 EKRERTSSSMERRSNGTASSSNLPASIFARSSTSFIN 236
Score = 29.0 bits (57), Expect(2) = 5e-045
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +3 / +3
Query: 339 GKDGRVDSTTNGAFNTLSGFEAAXXRNSW 425
G + V ++ TLSG+EAA RN+W
Sbjct: 567 GSEEVVHASGGYNLKTLSGYEAADERNNW 653
>gb|AW299129.1|AW299129 EST305803 KV2 Medicago truncatula cDNA clone KV2-12K8, mRNA
sequence
Length = 752
Score = 170 bits (366), Expect = 1e-041
Identities = 72/94 (76%), Positives = 84/94 (89%)
Frame = +3 / +3
Query: 12 LXNDVLDRAKIEAGKLELEAVPFDLRSLMDDVISLFSSKSREKCIELAVFVCDNVPKDVL 191
L N+VLDRAKIEAGKLELEAVPFDLRS++DDV+SLFS KSR K +ELAVFV D VP V+
Sbjct: 426 LINEVLDRAKIEAGKLELEAVPFDLRSILDDVLSLFSEKSRHKGLELAVFVSDKVPDIVM 605
Query: 192 GDPWRFRQILTNLVGNAVKFTERGHVFVRVCLAD 293
GDP RFRQI+TNLVGN+VKFTERGH+FV+V L++
Sbjct: 606 GDPGRFRQIVTNLVGNSVKFTERGHIFVKVHLSE 707
Score = 137 bits (294), Expect = 1e-031
Identities = 66/97 (68%), Positives = 69/97 (71%)
Frame = -1 / -2
Query: 301 FELSAKQTRTNT*PRSVNLTALPTKFVSICRNLQGSPRTSFGTLSHTNTASSMHFSRDFE 122
F S K T TN PRSVNLT LPT+FV+IC NL GSP T GTLS TNTASS DF
Sbjct: 715 FCFSDK*TLTNIWPRSVNLTELPTRFVTICLNLPGSPITISGTLSETNTASSKPLCLDFS 536
Query: 121 ENKEITSSMRERRSKGTASSSNFPASIFARSRTSFXN 11
E +E TSS ERRS GTASSSN PASIFARS TSF N
Sbjct: 535 EKRERTSSSMERRSNGTASSSNLPASIFARSSTSFIN 425
>gb|CX532373.1|CX532373 s13dNF61A03MJ017_271039 Methyl Jasmonate-Elicited Root Cell
Suspension Culture Medicago truncatula cDNA, mRNA
sequence
Length = 653
Score = 149 bits (320), Expect(2) = 7e-036
Identities = 60/94 (63%), Positives = 80/94 (85%)
Frame = +3 / +3
Query: 12 LXNDVLDRAKIEAGKLELEAVPFDLRSLMDDVISLFSSKSREKCIELAVFVCDNVPKDVL 191
L N+VLD+AKIE GKLELEAV FD+RS++DDV+SLFS KS+ K +ELA +V D VPK ++
Sbjct: 102 LINEVLDQAKIEFGKLELEAVLFDIRSILDDVLSLFSDKSQGKGVELAAYVSDQVPKLLI 281
Query: 192 GDPWRFRQILTNLVGNAVKFTERGHVFVRVCLAD 293
GDP RFRQI+TNL+GN++KFT++GH+FV + L +
Sbjct: 282 GDPGRFRQIITNLMGNSIKFTDKGHIFVTIHLVE 383
Score = 22.1 bits (42), Expect(2) = 7e-036
Identities = 9/18 (50%), Positives = 10/18 (55%)
Frame = +3 / +3
Query: 381 NTLSGFEAAXXRNSWQYF 434
NTLSGF SW+ F
Sbjct: 429 NTLSGFPVVKSCLSWKGF 482
>gb|AW191220.1|AW191220 T113394e KV2 Medicago truncatula cDNA clone pKV2-2B19, mRNA
sequence
Length = 469
Score = 133 bits (286), Expect(2) = 8e-034
Identities = 55/76 (72%), Positives = 66/76 (86%)
Frame = +3 / +2
Query: 69 AVPFDLRSLMDDVISLFSSKSREKCIELAVFVCDNVPKDVLGDPWRFRQILTNLVGNAVK 248
AVPFDLRS++DDV+SLFS K R K +ELAVFV D VP V+GDP RFRQI+TNLVGN+VK
Sbjct: 2 AVPFDLRSILDDVLSLFSEKCRHKGLELAVFVSDKVPDIVMGDPGRFRQIVTNLVGNSVK 181
Query: 249 FTERGHVFVRVCLADN 296
FTERGH+FV+V L++N
Sbjct: 182 FTERGHIFVKVHLSEN 229
Score = 30.9 bits (61), Expect(2) = 8e-034
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = +3 / +2
Query: 339 GKDGRVDSTTNGAFNTLSGFEAAXXRNSWQYF 434
G + V ++ TLSG+EAA RN+W F
Sbjct: 275 GSEEVVHASGGYNLKTLSGYEAADERNNWDNF 370
>gb|AC147538.16| Medicago truncatula clone mth2-135c7, WORKING DRAFT SEQUENCE, 3 ordered
pieces
Length = 133436
Score = 79.0 bits (166), Expect(4) = 3e-032
Identities = 31/51 (60%), Positives = 45/51 (88%)
Frame = +3 / -2
Query: 12 LXNDVLDRAKIEAGKLELEAVPFDLRSLMDDVISLFSSKSREKCIELAVFV 164
+ N+VLD+AKIE+GK+ELEAV FD+RS+MDDV+SLFS KS+ K +E+ +++
Sbjct: 60754 IINEVLDQAKIESGKIELEAVLFDIRSIMDDVLSLFSEKSQGKGVEVIIYL 60602
Score = 58.3 bits (121), Expect(4) = 3e-032
Identities = 21/34 (61%), Positives = 30/34 (88%)
Frame = +3 / -2
Query: 147 ELAVFVCDNVPKDVLGDPWRFRQILTNLVGNAVK 248
+LAV+V D VP+ ++GDP RFRQI+TNL+GN++K
Sbjct: 60505 KLAVYVSDQVPQQLIGDPGRFRQIITNLMGNSIK 60404
Score = 32.2 bits (64), Expect(4) = 3e-032
Identities = 13/26 (50%), Positives = 17/26 (65%)
Frame = +3 / -1
Query: 357 DSTTNGAFNTLSGFEAAXXRNSWQYF 434
+ST+ A +TLSGF A R SW+ F
Sbjct: 60224 ESTSKDAEHTLSGFPVADGRRSWEGF 60147
Score = 24.9 bits (48), Expect(4) = 3e-032
Identities = 7/16 (43%), Positives = 13/16 (81%)
Frame = +3 / -1
Query: 246 KFTERGHVFVRVCLAD 293
+FT++GH+FV + L +
Sbjct: 60302 QFTDKGHIFVTIHLVE 60255
>gb|AC147538.15| Medicago truncatula clone mth2-135c7, WORKING DRAFT SEQUENCE, 5 ordered
pieces
Length = 133186
Score = 79.0 bits (166), Expect(4) = 3e-032
Identities = 31/51 (60%), Positives = 45/51 (88%)
Frame = +3 / -3
Query: 12 LXNDVLDRAKIEAGKLELEAVPFDLRSLMDDVISLFSSKSREKCIELAVFV 164
+ N+VLD+AKIE+GK+ELEAV FD+RS+MDDV+SLFS KS+ K +E+ +++
Sbjct: 61022 IINEVLDQAKIESGKIELEAVLFDIRSIMDDVLSLFSEKSQGKGVEVIIYL 60870
Score = 58.3 bits (121), Expect(4) = 3e-032
Identities = 21/34 (61%), Positives = 30/34 (88%)
Frame = +3 / -3
Query: 147 ELAVFVCDNVPKDVLGDPWRFRQILTNLVGNAVK 248
+LAV+V D VP+ ++GDP RFRQI+TNL+GN++K
Sbjct: 60773 KLAVYVSDQVPQQLIGDPGRFRQIITNLMGNSIK 60672
Score = 32.2 bits (64), Expect(4) = 3e-032
Identities = 13/26 (50%), Positives = 17/26 (65%)
Frame = +3 / -2
Query: 357 DSTTNGAFNTLSGFEAAXXRNSWQYF 434
+ST+ A +TLSGF A R SW+ F
Sbjct: 60492 ESTSKDAEHTLSGFPVADGRRSWEGF 60415
Score = 24.9 bits (48), Expect(4) = 3e-032
Identities = 7/16 (43%), Positives = 13/16 (81%)
Frame = +3 / -2
Query: 246 KFTERGHVFVRVCLAD 293
+FT++GH+FV + L +
Sbjct: 60570 QFTDKGHIFVTIHLVE 60523
>gb|BI308772.1|BI308772 EST530182 GPOD Medicago truncatula cDNA clone pGPOD-8N21 5' end,
mRNA sequence
Length = 812
Score = 114 bits (243), Expect = 1e-024
Identities = 49/78 (62%), Positives = 65/78 (83%)
Frame = +3 / +1
Query: 18 NDVLDRAKIEAGKLELEAVPFDLRSLMDDVISLFSSKSREKCIELAVFVCDNVPKDVLGD 197
++VL +AKIEAGKLELEAV F +++D+V+SLFS KS EK IEL+V+ + VP+ V+GD
Sbjct: 505 SEVLYQAKIEAGKLELEAVAFHPHAILDEVLSLFSEKSDEKGIELSVYASNQVPEVVIGD 684
Query: 198 PWRFRQILTNLVGNAVKF 251
P RFRQI+TNLVGN++KF
Sbjct: 685 PKRFRQIITNLVGNSLKF 738
>gb|AC170800.5| Medicago truncatula clone mth2-32n7, complete sequence
Length = 99712
Score = 89.5 bits (189), Expect(3) = 3e-021
Identities = 39/57 (68%), Positives = 46/57 (80%)
Frame = +3 / +3
Query: 12 LXNDVLDRAKIEAGKLELEAVPFDLRSLMDDVISLFSSKSREKCIELAVFVCDNVPK 182
L N+VLDRAKIEAGKLELEAVPFDLRS++DDV+SLFS KSR K +E+ V + K
Sbjct: 41115 LINEVLDRAKIEAGKLELEAVPFDLRSILDDVLSLFSEKSRHKGLEVCSIVTKPIVK 41285
Score = 35.4 bits (71), Expect(3) = 3e-021
Identities = 12/20 (60%), Positives = 18/20 (90%)
Frame = +3 / +1
Query: 237 NAVKFTERGHVFVRVCLADN 296
N ++FTERGH+FV+V L++N
Sbjct: 41617 NILQFTERGHIFVKVHLSEN 41676
Score = 30.9 bits (61), Expect(3) = 3e-021
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = +3 / +1
Query: 339 GKDGRVDSTTNGAFNTLSGFEAAXXRNSWQYF 434
G + V ++ TLSG+EAA RN+W F
Sbjct: 41722 GSEEVVHASGGYNLKTLSGYEAADERNNWDNF 41817
>gb|AC141922.19| Medicago truncatula clone mth2-9n11, complete sequence
Length = 107448
Score = 89.5 bits (189), Expect(3) = 4e-021
Identities = 39/57 (68%), Positives = 46/57 (80%)
Frame = +3 / +3
Query: 12 LXNDVLDRAKIEAGKLELEAVPFDLRSLMDDVISLFSSKSREKCIELAVFVCDNVPK 182
L N+VLDRAKIEAGKLELEAVPFDLRS++DDV+SLFS KSR K +E+ V + K
Sbjct: 51597 LINEVLDRAKIEAGKLELEAVPFDLRSILDDVLSLFSEKSRHKGLEVCSIVTKPIVK 51767
Score = 35.4 bits (71), Expect(3) = 4e-021
Identities = 12/20 (60%), Positives = 18/20 (90%)
Frame = +3 / +1
Query: 237 NAVKFTERGHVFVRVCLADN 296
N ++FTERGH+FV+V L++N
Sbjct: 52099 NILQFTERGHIFVKVHLSEN 52158
Score = 30.9 bits (61), Expect(3) = 4e-021
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = +3 / +1
Query: 339 GKDGRVDSTTNGAFNTLSGFEAAXXRNSWQYF 434
G + V ++ TLSG+EAA RN+W F
Sbjct: 52204 GSEEVVHASGGYNLKTLSGYEAADERNNWDNF 52299
>gb|AC144931.28| Medicago truncatula clone mth2-22b18, complete sequence
Length = 122821
Score = 89.5 bits (189), Expect(3) = 5e-021
Identities = 39/57 (68%), Positives = 46/57 (80%)
Frame = +3 / +2
Query: 12 LXNDVLDRAKIEAGKLELEAVPFDLRSLMDDVISLFSSKSREKCIELAVFVCDNVPK 182
L N+VLDRAKIEAGKLELEAVPFDLRS++DDV+SLFS KSR K +E+ V + K
Sbjct: 23132 LINEVLDRAKIEAGKLELEAVPFDLRSILDDVLSLFSEKSRHKGLEVCSIVTKPIVK 23302
Score = 35.4 bits (71), Expect(3) = 5e-021
Identities = 12/20 (60%), Positives = 18/20 (90%)
Frame = +3 / +3
Query: 237 NAVKFTERGHVFVRVCLADN 296
N ++FTERGH+FV+V L++N
Sbjct: 23634 NILQFTERGHIFVKVHLSEN 23693
Score = 30.9 bits (61), Expect(3) = 5e-021
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = +3 / +3
Query: 339 GKDGRVDSTTNGAFNTLSGFEAAXXRNSWQYF 434
G + V ++ TLSG+EAA RN+W F
Sbjct: 23739 GSEEVVHASGGYNLKTLSGYEAADERNNWDNF 23834
>gb|AC142094.12| Medicago truncatula clone mth2-34h6, complete sequence
Length = 151594
Score = 89.5 bits (189), Expect(3) = 7e-021
Identities = 39/57 (68%), Positives = 46/57 (80%)
Frame = +3 / -2
Query: 12 LXNDVLDRAKIEAGKLELEAVPFDLRSLMDDVISLFSSKSREKCIELAVFVCDNVPK 182
L N+VLDRAKIEAGKLELEAVPFDLRS++DDV+SLFS KSR K +E+ V + K
Sbjct: 128463 LINEVLDRAKIEAGKLELEAVPFDLRSILDDVLSLFSEKSRHKGLEVCSIVTKPIVK 128293
Score = 35.4 bits (71), Expect(3) = 7e-021
Identities = 12/20 (60%), Positives = 18/20 (90%)
Frame = +3 / -3
Query: 237 NAVKFTERGHVFVRVCLADN 296
N ++FTERGH+FV+V L++N
Sbjct: 127961 NILQFTERGHIFVKVHLSEN 127902
Score = 30.9 bits (61), Expect(3) = 7e-021
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = +3 / -3
Query: 339 GKDGRVDSTTNGAFNTLSGFEAAXXRNSWQYF 434
G + V ++ TLSG+EAA RN+W F
Sbjct: 127856 GSEEVVHASGGYNLKTLSGYEAADERNNWDNF 127761
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 467,764,895
Number of Sequences: 392609
Number of extensions: 6377059
Number of successful extensions: 48070
Number of sequences better than 1.0e-020: 14
length of database: 147,244,331
effective HSP length: 53
effective length of database: 126,436,054
effective search space used: 15930942804
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)