TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAT2e04.yg.2.1
(424 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BM778939.1|BM778939 EST589514 KV2 Medicago truncatula cD... 253 8e-067
gb|CA917254.1|CA917254 EST641401 GPOD Medicago truncatula c... 224 7e-058
gb|AC144478.20| Medicago truncatula clone mth2-20b20, compl... 130 1e-052
gb|AC127428.38| Medicago truncatula clone mth2-36c19, WORKI... 130 4e-052
gb|BG448764.1|BG448764 NF045B07NR1F1058 Nodulated root Medi... 156 2e-037
gb|CG963332.1|CG963332 MBEEP63TRB mth2 Medicago truncatula ... 87 1e-023
gb|CG976296.1|CG976296 MBEEP74TRB mth2 Medicago truncatula ... 87 1e-023
>gb|BM778939.1|BM778939 EST589514 KV2 Medicago truncatula cDNA clone pKV2-20A8, mRNA
sequence
Length = 675
Score = 253 bits (548), Expect = 8e-067
Identities = 101/139 (72%), Positives = 122/139 (87%)
Frame = +3 / +1
Query: 3 ATALPKAHPSFSLQQVLEMSERWKARTVPEEPIHLFIGILSATNHFAERMAIRKTWMQFP 182
AT+LP +HPSFS Q+VLEMSE WKA +P+ PI LFIG+LSA+NHFAERMA+RKTWMQ
Sbjct: 1 ATSLPTSHPSFSPQRVLEMSETWKASALPKHPIRLFIGVLSASNHFAERMAVRKTWMQDA 180
Query: 183 AIQSGNAVARFFVALSHRKEINAALKKEAEYFGDIVILPFIDRYELVVLKTVAICQYGVH 362
AI+S + V RFFVAL+ RKE+NA L+KEA YFGDIVILPF+DRYELVV+KT+AIC++G+
Sbjct: 181 AIKSSDVVVRFFVALNPRKEVNAVLRKEAAYFGDIVILPFMDRYELVVIKTIAICEFGIQ 360
Query: 363 NVTADYIMKCDDDTFVRLD 419
NVTA +IMKCDDDTFVR+D
Sbjct: 361 NVTAAHIMKCDDDTFVRVD 417
Score = 136 bits (291), Expect = 2e-031
Identities = 71/140 (50%), Positives = 82/140 (58%)
Frame = -1 / -2
Query: 424 TISSRTKVSSSHFMM*SAVTLCTPY*HIATVFRTTSSYRSMNGRMTMSPKYXXXXXXXXX 245
T+S+ TKVSSSHFM+ +AVT P IA V TTSS RS+ G+MT+SPK
Sbjct: 422 TVSTLTKVSSSHFMICAAVTF*IPNSQIAMVLITTSS*RSIKGKMTISPK*AASFLSTAF 243
Query: 244 XXXXXXXATKNRATAFPDWIAGNCIQVFLIAMRSAKWFVAERMPMKRWIGSSGTVRAFHL 65
ATKNR T D IA +CI VF A+RSAK F AER P+K IG G AF +
Sbjct: 242 TSFLGFNATKNRTTTSEDLIAASCIHVFRTAIRSAK*FEAERTPIKSLIGCLGRALAFQV 63
Query: 64 SDISKTCWREKLGCAFGSAV 5
SDIS T EKLGC G V
Sbjct: 62 SDISSTL*GEKLGCEVGRDV 3
>gb|CA917254.1|CA917254 EST641401 GPOD Medicago truncatula cDNA clone GPOD-35J3, mRNA
sequence
Length = 831
Score = 224 bits (483), Expect = 7e-058
Identities = 90/139 (64%), Positives = 112/139 (80%)
Frame = +3 / +1
Query: 3 ATALPKAHPSFSLQQVLEMSERWKARTVPEEPIHLFIGILSATNHFAERMAIRKTWMQFP 182
A +LP +HPSF+ Q LE+ RWKA + + + LFIGILSA NHFAERMA+RK+WMQ
Sbjct: 334 AASLPTSHPSFAPQMHLELLPRWKAPPILDVNVELFIGILSAGNHFAERMAVRKSWMQHK 513
Query: 183 AIQSGNAVARFFVALSHRKEINAALKKEAEYFGDIVILPFIDRYELVVLKTVAICQYGVH 362
I+S + VARFFVAL RK+IN +KKEAEYFGDI+I+P++D Y+LVVLKTVAIC+YG+
Sbjct: 514 LIKSSHVVARFFVALHGRKDINVEIKKEAEYFGDIIIVPYMDHYDLVVLKTVAICEYGIR 693
Query: 363 NVTADYIMKCDDDTFVRLD 419
+V A YIMKCDDDTFVR+D
Sbjct: 694 SVAAKYIMKCDDDTFVRVD 750
Score = 117 bits (250), Expect = 1e-025
Identities = 65/141 (46%), Positives = 76/141 (53%)
Frame = -1 / -2
Query: 424 TISSRTKVSSSHFMM*SAVTLCTPY*HIATVFRTTSSYRSMNGRMTMSPKYXXXXXXXXX 245
T S+ T VSSSHFM+ A T PY H+ATVF+TT S SM G + +SPKY
Sbjct: 755 TESTLTNVSSSHFMIYLAATERIPYSHMATVFKTTRS**SMYGTIIISPKYSASFFISTF 576
Query: 244 XXXXXXXATKNRATAFPDWIAGNCIQVFLIAMRSAKWFVAERMPMKRWIGSSGTVRAFHL 65
ATKNRAT D I+ CI FL A+RSAKW AER+PMK +S AFH
Sbjct: 575 ISFLPCKATKNRATT*EDLISLCCIHDFLTAIRSAKWLPAERIPMKSSTFTSRIGGAFHR 396
Query: 64 SDISKTCWREKLGCAFGSAVA 2
S+ KLGC G A
Sbjct: 395 GSNSRCI*GAKLGCDVGKEAA 333
>gb|AC144478.20| Medicago truncatula clone mth2-20b20, complete sequence
Length = 144248
Score = 130 bits (279), Expect(3) = 1e-052
Identities = 53/75 (70%), Positives = 63/75 (84%)
Frame = +3 / -2
Query: 3 ATALPKAHPSFSLQQVLEMSERWKARTVPEEPIHLFIGILSATNHFAERMAIRKTWMQFP 182
AT+LP +HPSFS Q+VLEMSE WKA +P+ PI LFIG+LSA+NHFAERMA+RKTWMQ
Sbjct: 9823 ATSLPTSHPSFSPQRVLEMSETWKASALPKHPIRLFIGVLSASNHFAERMAVRKTWMQDA 9644
Query: 183 AIQSGNAVARFFVAL 227
AI+S + V RFFVAL
Sbjct: 9643 AIKSSDVVVRFFVAL 9599
Score = 87.2 bits (184), Expect(3) = 1e-052
Identities = 33/42 (78%), Positives = 40/42 (95%)
Frame = +3 / -2
Query: 234 RKEINAALKKEAEYFGDIVILPFIDRYELVVLKTVAICQYGV 359
RKE+NA L+KEA YFGDIVILPF+DRYELVV+KT+AIC++GV
Sbjct: 7888 RKEVNAVLRKEAAYFGDIVILPFMDRYELVVIKTIAICEFGV 7763
Score = 84.0 bits (177), Expect(3) = 2e-022
Identities = 41/73 (56%), Positives = 45/73 (61%)
Frame = -1 / +3
Query: 220 TKNRATAFPDWIAGNCIQVFLIAMRSAKWFVAERMPMKRWIGSSGTVRAFHLSDISKTCW 41
TKNR T D IA +CI VF A+RSAK F AER P+K IG G AF +SDIS T
Sbjct: 9606 TKNRTTTSEDLIAASCIHVFRTAIRSAK*FEAERTPIKSLIGCLGRALAFQVSDISSTL* 9785
Query: 40 REKLGCAFGSAVA 2
EKLGC G VA
Sbjct: 9786 GEKLGCEVGRDVA 9824
Score = 44.1 bits (90), Expect(3) = 1e-052
Identities = 16/21 (76%), Positives = 19/21 (90%)
Frame = +3 / -2
Query: 357 VHNVTADYIMKCDDDTFVRLD 419
+ NVTA +IMKCDDDTFVR+D
Sbjct: 7636 IQNVTAAHIMKCDDDTFVRVD 7574
Score = 41.8 bits (85), Expect(3) = 2e-022
Identities = 20/42 (47%), Positives = 26/42 (61%)
Frame = -3 / +2
Query: 359 HPVLTYRNRFQNH*LIPVDERQNDYVSKILCFLLQCCIYLLS 234
+P LT N F ++ LI V + QND ++KI L Q CIY LS
Sbjct: 7763 YPKLTDSNGFNHNKLIAVHKGQNDNITKISSLLPQHCIYFLS 7888
Score = 33.6 bits (67), Expect(3) = 2e-022
Identities = 14/20 (70%), Positives = 18/20 (90%)
Frame = -1 / +3
Query: 424 TISSRTKVSSSHFMM*SAVT 365
T+S+ TKVSSSHFM+ +AVT
Sbjct: 7569 TVSTLTKVSSSHFMICAAVT 7628
>gb|AC127428.38| Medicago truncatula clone mth2-36c19, WORKING DRAFT SEQUENCE, 3 ordered
pieces
Length = 274828
Score = 130 bits (279), Expect(3) = 4e-052
Identities = 53/75 (70%), Positives = 63/75 (84%)
Frame = +3 / +2
Query: 3 ATALPKAHPSFSLQQVLEMSERWKARTVPEEPIHLFIGILSATNHFAERMAIRKTWMQFP 182
AT+LP +HPSFS Q+VLEMSE WKA +P+ PI LFIG+LSA+NHFAERMA+RKTWMQ
Sbjct: 272213 ATSLPTSHPSFSPQRVLEMSETWKASALPKHPIRLFIGVLSASNHFAERMAVRKTWMQDA 272392
Query: 183 AIQSGNAVARFFVAL 227
AI+S + V RFFVAL
Sbjct: 272393 AIKSSDVVVRFFVAL 272437
Score = 87.2 bits (184), Expect(3) = 4e-052
Identities = 33/42 (78%), Positives = 40/42 (95%)
Frame = +3 / +2
Query: 234 RKEINAALKKEAEYFGDIVILPFIDRYELVVLKTVAICQYGV 359
RKE+NA L+KEA YFGDIVILPF+DRYELVV+KT+AIC++GV
Sbjct: 274148 RKEVNAVLRKEAAYFGDIVILPFMDRYELVVIKTIAICEFGV 274273
Score = 84.0 bits (177), Expect(3) = 7e-022
Identities = 41/73 (56%), Positives = 45/73 (61%)
Frame = -1 / -2
Query: 220 TKNRATAFPDWIAGNCIQVFLIAMRSAKWFVAERMPMKRWIGSSGTVRAFHLSDISKTCW 41
TKNR T D IA +CI VF A+RSAK F AER P+K IG G AF +SDIS T
Sbjct: 272430 TKNRTTTSEDLIAASCIHVFRTAIRSAK*FEAERTPIKSLIGCLGRALAFQVSDISSTL* 272251
Query: 40 REKLGCAFGSAVA 2
EKLGC G VA
Sbjct: 272250 GEKLGCEVGRDVA 272212
Score = 44.1 bits (90), Expect(3) = 4e-052
Identities = 16/21 (76%), Positives = 19/21 (90%)
Frame = +3 / +2
Query: 357 VHNVTADYIMKCDDDTFVRLD 419
+ NVTA +IMKCDDDTFVR+D
Sbjct: 274400 IQNVTAAHIMKCDDDTFVRVD 274462
Score = 41.8 bits (85), Expect(3) = 7e-022
Identities = 20/42 (47%), Positives = 26/42 (61%)
Frame = -3 / -1
Query: 359 HPVLTYRNRFQNH*LIPVDERQNDYVSKILCFLLQCCIYLLS 234
+P LT N F ++ LI V + QND ++KI L Q CIY LS
Sbjct: 274273 YPKLTDSNGFNHNKLIAVHKGQNDNITKISSLLPQHCIYFLS 274148
Score = 33.6 bits (67), Expect(3) = 7e-022
Identities = 14/20 (70%), Positives = 18/20 (90%)
Frame = -1 / -2
Query: 424 TISSRTKVSSSHFMM*SAVT 365
T+S+ TKVSSSHFM+ +AVT
Sbjct: 274467 TVSTLTKVSSSHFMICAAVT 274408
>gb|BG448764.1|BG448764 NF045B07NR1F1058 Nodulated root Medicago truncatula cDNA clone
NF045B07NR 5', mRNA sequence
Length = 678
Score = 156 bits (335), Expect = 2e-037
Identities = 64/93 (68%), Positives = 76/93 (81%)
Frame = +3 / +1
Query: 3 ATALPKAHPSFSLQQVLEMSERWKARTVPEEPIHLFIGILSATNHFAERMAIRKTWMQFP 182
AT+LP +HPSFS Q+VLEMSE WKA +P+ PI LFIG+LSA+NHFAERMA+RKTWMQ
Sbjct: 400 ATSLPTSHPSFSPQRVLEMSETWKASALPKHPIRLFIGVLSASNHFAERMAVRKTWMQDA 579
Query: 183 AIQSGNAVARFFVALSHRKEINAALKKEAEYFG 281
AI+S + V RFFVAL+ RKE+NA L K A YFG
Sbjct: 580 AIKSSDVVVRFFVALNPRKEVNAVLXKXAAYFG 678
>gb|CG963332.1|CG963332 MBEEP63TRB mth2 Medicago truncatula genomic clone 39L6, DNA
sequence
Length = 827
Score = 87.2 bits (184), Expect(2) = 1e-023
Identities = 33/42 (78%), Positives = 40/42 (95%)
Frame = +3 / -1
Query: 234 RKEINAALKKEAEYFGDIVILPFIDRYELVVLKTVAICQYGV 359
RKE+NA L+KEA YFGDIVILPF+DRYELVV+KT+AIC++GV
Sbjct: 590 RKEVNAVLRKEAAYFGDIVILPFMDRYELVVIKTIAICEFGV 465
Score = 44.1 bits (90), Expect(2) = 1e-023
Identities = 16/21 (76%), Positives = 19/21 (90%)
Frame = +3 / -1
Query: 357 VHNVTADYIMKCDDDTFVRLD 419
+ NVTA +IMKCDDDTFVR+D
Sbjct: 338 IQNVTAAHIMKCDDDTFVRVD 276
>gb|CG976296.1|CG976296 MBEEP74TRB mth2 Medicago truncatula genomic clone 39N4, DNA
sequence
Length = 888
Score = 87.2 bits (184), Expect(2) = 1e-023
Identities = 33/42 (78%), Positives = 40/42 (95%)
Frame = +3 / -1
Query: 234 RKEINAALKKEAEYFGDIVILPFIDRYELVVLKTVAICQYGV 359
RKE+NA L+KEA YFGDIVILPF+DRYELVV+KT+AIC++GV
Sbjct: 684 RKEVNAVLRKEAAYFGDIVILPFMDRYELVVIKTIAICEFGV 559
Score = 44.1 bits (90), Expect(2) = 1e-023
Identities = 16/21 (76%), Positives = 19/21 (90%)
Frame = +3 / -1
Query: 357 VHNVTADYIMKCDDDTFVRLD 419
+ NVTA +IMKCDDDTFVR+D
Sbjct: 432 IQNVTAAHIMKCDDDTFVRVD 370
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 447,538,442
Number of Sequences: 392609
Number of extensions: 5843886
Number of successful extensions: 93253
Number of sequences better than 1.0e-020: 9
length of database: 147,244,331
effective HSP length: 52
effective length of database: 126,828,663
effective search space used: 11160922344
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)