TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAO3g02.yg.2.1
(344 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG450408.1|BG450408 NF014E01DT1F1006 Drought Medicago tr... 73 2e-025
gb|BG452084.1|BG452084 NF084E03LF1F1023 Developing leaf Med... 73 2e-025
gb|AJ621857.1|AJ621857 AJ621857 Medicago truncatula J5 root... 73 5e-025
gb|BG453707.1|BG453707 NF100E09LF1F1068 Developing leaf Med... 73 2e-024
gb|AW560247.1|AW560247 EST315295 DSIR Medicago truncatula c... 70 5e-024
gb|BE943506.1|BE943506 EST423085 MGHG Medicago truncatula c... 65 6e-023
gb|AW329404.2|AW329404 N200637e rootphos(-) Medicago trunca... 67 2e-022
>gb|BG450408.1|BG450408 NF014E01DT1F1006 Drought Medicago truncatula cDNA clone NF014E01DT
5', mRNA sequence
Length = 594
Score = 73.5 bits (154), Expect(2) = 2e-025
Identities = 29/51 (56%), Positives = 32/51 (62%)
Frame = +1 / +2
Query: 16 YLFDSTNDVSVLPXXHTIHVKCLKEMEEHCQFACPLCSXXXXXMXXXXERL 168
YLF+S NDV V+P HTIH CL EM EH QF CPLCS M E+L
Sbjct: 74 YLFESRNDVIVMPCGHTIHKSCLNEMREHFQFTCPLCSKSVCDMSKVWEKL 226
Score = 61.1 bits (127), Expect(2) = 2e-025
Identities = 23/35 (65%), Positives = 27/35 (77%)
Frame = +1 / +2
Query: 205 HKMVRILCNDCGAISDVQFHLIAHKCQNXXSYNTR 309
+KMV ILCNDCG S+V+FH +A KC N SYNTR
Sbjct: 269 NKMVWILCNDCGKTSNVRFHFVAQKCLNCNSYNTR 373
>gb|BG452084.1|BG452084 NF084E03LF1F1023 Developing leaf Medicago truncatula cDNA clone
NF084E03LF 5', mRNA sequence
Length = 618
Score = 73.5 bits (154), Expect(2) = 2e-025
Identities = 29/51 (56%), Positives = 32/51 (62%)
Frame = +1 / +3
Query: 16 YLFDSTNDVSVLPXXHTIHVKCLKEMEEHCQFACPLCSXXXXXMXXXXERL 168
YLF+S NDV V+P HTIH CL EM EH QF CPLCS M E+L
Sbjct: 117 YLFESRNDVIVMPCGHTIHKSCLNEMREHFQFTCPLCSKSVCDMSKVWEKL 269
Score = 61.1 bits (127), Expect(2) = 2e-025
Identities = 23/35 (65%), Positives = 27/35 (77%)
Frame = +1 / +3
Query: 205 HKMVRILCNDCGAISDVQFHLIAHKCQNXXSYNTR 309
+KMV ILCNDCG S+V+FH +A KC N SYNTR
Sbjct: 312 NKMVWILCNDCGKTSNVRFHFVAQKCLNCNSYNTR 416
>gb|AJ621857.1|AJ621857 AJ621857 Medicago truncatula J5 roots Medicago truncatula cDNA
clone MtGmEs376, mRNA sequence
Length = 591
Score = 73.0 bits (153), Expect(2) = 5e-025
Identities = 29/51 (56%), Positives = 33/51 (64%)
Frame = +1 / -1
Query: 16 YLFDSTNDVSVLPXXHTIHVKCLKEMEEHCQFACPLCSXXXXXMXXXXERL 168
YLF+S NDVSVL HTIH CL EM EH Q+ACPLCS M E++
Sbjct: 396 YLFESVNDVSVLHCGHTIHKSCLNEMREHFQYACPLCSKSVCDMSKVWEKI 244
Score = 60.2 bits (125), Expect(2) = 5e-025
Identities = 24/35 (68%), Positives = 26/35 (74%)
Frame = +1 / -1
Query: 205 HKMVRILCNDCGAISDVQFHLIAHKCQNXXSYNTR 309
+K V ILCNDCG S VQFHL+A KC N SYNTR
Sbjct: 201 NKTVWILCNDCGENSHVQFHLVAQKCLNCKSYNTR 97
>gb|BG453707.1|BG453707 NF100E09LF1F1068 Developing leaf Medicago truncatula cDNA clone
NF100E09LF 5', mRNA sequence
Length = 644
Score = 73.5 bits (154), Expect(2) = 2e-024
Identities = 29/52 (55%), Positives = 34/52 (65%)
Frame = +1 / +2
Query: 13 RYLFDSTNDVSVLPXXHTIHVKCLKEMEEHCQFACPLCSXXXXXMXXXXERL 168
+YLF+S NDVSVL HTIH CL EM EH Q+ACPLCS M E++
Sbjct: 344 QYLFESVNDVSVLHCGHTIHKSCLNEMREHFQYACPLCSKSVCDMSKVWEKI 499
Score = 57.9 bits (120), Expect(2) = 2e-024
Identities = 23/34 (67%), Positives = 25/34 (73%)
Frame = +1 / +2
Query: 205 HKMVRILCNDCGAISDVQFHLIAHKCQNXXSYNT 306
+K V ILCNDCG S VQFHL+A KC N SYNT
Sbjct: 542 NKTVWILCNDCGENSHVQFHLVAQKCLNCKSYNT 643
>gb|AW560247.1|AW560247 EST315295 DSIR Medicago truncatula cDNA clone pDSIR-26F15, mRNA
sequence
Length = 629
Score = 69.8 bits (146), Expect(2) = 5e-024
Identities = 28/50 (56%), Positives = 32/50 (64%)
Frame = +1 / -3
Query: 19 LFDSTNDVSVLPXXHTIHVKCLKEMEEHCQFACPLCSXXXXXMXXXXERL 168
LF+S NDVSVL HTIH CL EM EH Q+ACPLCS M E++
Sbjct: 627 LFESVNDVSVLHCGHTIHKSCLNEMREHFQYACPLCSKSVCDMSKVWEKI 478
Score = 60.2 bits (125), Expect(2) = 5e-024
Identities = 24/35 (68%), Positives = 26/35 (74%)
Frame = +1 / -3
Query: 205 HKMVRILCNDCGAISDVQFHLIAHKCQNXXSYNTR 309
+K V ILCNDCG S VQFHL+A KC N SYNTR
Sbjct: 435 NKTVWILCNDCGENSHVQFHLVAQKCLNCKSYNTR 331
>gb|BE943506.1|BE943506 EST423085 MGHG Medicago truncatula cDNA clone pMGHG-15P14, mRNA
sequence
Length = 459
Score = 64.8 bits (135), Expect(2) = 6e-023
Identities = 26/47 (55%), Positives = 28/47 (59%)
Frame = +1 / +1
Query: 28 STNDVSVLPXXHTIHVKCLKEMEEHCQFACPLCSXXXXXMXXXXERL 168
S NDV V+P HTIH CL EM EH QF CPLCS M E+L
Sbjct: 1 SRNDVIVMPCGHTIHKSCLNEMREHFQFTCPLCSKSVCDMSKVWEKL 141
Score = 61.1 bits (127), Expect(2) = 6e-023
Identities = 23/35 (65%), Positives = 27/35 (77%)
Frame = +1 / +1
Query: 205 HKMVRILCNDCGAISDVQFHLIAHKCQNXXSYNTR 309
+KMV ILCNDCG S+V+FH +A KC N SYNTR
Sbjct: 184 NKMVWILCNDCGKTSNVRFHFVAQKCLNCNSYNTR 288
>gb|AW329404.2|AW329404 N200637e rootphos(-) Medicago truncatula cDNA clone MHRP-19G1, mRNA
sequence
Length = 590
Score = 66.6 bits (139), Expect(2) = 2e-022
Identities = 24/51 (47%), Positives = 32/51 (62%)
Frame = +1 / +3
Query: 16 YLFDSTNDVSVLPXXHTIHVKCLKEMEEHCQFACPLCSXXXXXMXXXXERL 168
Y+F+ST ++ V+P HTIH C+ EM EH ++ CPLCS M ERL
Sbjct: 195 YIFESTKEIVVMPCGHTIHKSCIDEMREHHRYTCPLCSKSFCDMSRVWERL 347
Score = 57.9 bits (120), Expect(2) = 2e-022
Identities = 22/41 (53%), Positives = 28/41 (68%)
Frame = +1 / +3
Query: 187 LSDSCDHKMVRILCNDCGAISDVQFHLIAHKCQNXXSYNTR 309
+ + +KMV ILCNDCG +V+FH +A KC N SYNTR
Sbjct: 372 MPEQYQNKMVWILCNDCGNTGNVRFHFVALKCLNCNSYNTR 494
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 273,009,701
Number of Sequences: 392609
Number of extensions: 2469173
Number of successful extensions: 73720
Number of sequences better than 1.0e-020: 9
length of database: 147,244,331
effective HSP length: 51
effective length of database: 127,221,272
effective search space used: 8014940136
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)