TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF36h01.yg.2.1
(689 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF649782.1|BF649782 NF084A11EC1F1083 Elicited cell cultu... 227 e-108
gb|AC151000.3| Medicago truncatula chromosome 2 BAC clone m... 199 2e-097
gb|BI308315.1|BI308315 EST529725 GPOD Medicago truncatula c... 199 3e-089
emb|CT030180.3| M.truncatula DNA sequence from clone MTH2-1... 95 1e-061
gb|AC144618.7| Medicago truncatula clone mth2-21d12, comple... 95 1e-061
gb|BI270588.1|BI270588 NF010B12FL1F1101 Developing flower M... 199 3e-053
gb|AC135102.16| Medicago truncatula clone mth2-8m3, complet... 167 2e-040
gb|CG936178.1|CG936178 MBENI74TR mth2 Medicago truncatula g... 152 6e-036
gb|AY308955.1| Medicago truncatula cellulase mRNA, complete... 66 9e-034
gb|BQ165567.1|BQ165567 EST611436 KVKC Medicago truncatula c... 66 9e-034
gb|AJ845540.1|AJ845540 AJ845540 MtSNF Medicago truncatula c... 134 1e-030
gb|AJ845845.1|AJ845845 AJ845845 MtSNF Medicago truncatula c... 112 3e-024
gb|AC150784.20| Medicago truncatula clone mth2-134j11, WORK... 53 4e-023
gb|AC127169.13| Medicago truncatula clone mth2-36n3, comple... 70 2e-022
>gb|BF649782.1|BF649782 NF084A11EC1F1083 Elicited cell culture Medicago truncatula cDNA
clone NF084A11EC 5', mRNA sequence
Length = 666
Score = 227 bits (490), Expect(2) = e-108
Identities = 90/104 (86%), Positives = 97/104 (93%)
Frame = -2 / +1
Query: 313 WFGSPNYGVFTWDDKLPGAQVLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSYLPVFNS 134
++G P+YGVF+WD+KL GAQVLLSRLRLFLSPGYPYEEILRTFHNQT VMCSYLPVF S
Sbjct: 301 FWGGPDYGVFSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPVFTS 480
Query: 133 FNFTKGGLIQLNHGRPQPLQYAVNAAFLASLYSDYLEAADTPGW 2
FN TKGGLIQLNHGRPQPLQY VNAAFLA+LYSDYL+AADTPGW
Sbjct: 481 FNRTKGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLDAADTPGW 612
Score = 186 bits (400), Expect(2) = e-108
Identities = 74/104 (71%), Positives = 81/104 (77%)
Frame = -2 / +1
Query: 616 HSCSDLAXXXXAXXXXASIVFKDSKTYSDKLVKGAKALYKFGRLQRGRYSPNGSDQAIFY 437
HSCSDLA A ASIVFKD+K YS KLV GA L+KF R QRGRYSP S+ A FY
Sbjct: 1 HSCSDLAAEMAAALAAASIVFKDNKAYSKKLVHGATTLFKFSRDQRGRYSPGRSEAATFY 180
Query: 436 NSTSYWDEFVWGGAWMYFATGNNSYLTVATAPGMAKHAGAYWFG 305
NST Y+DEFVWGGAWMYFATGNNSYL +AT PG+AKHAGA+W G
Sbjct: 181 NSTGYFDEFVWGGAWMYFATGNNSYLKLATTPGIAKHAGAFWGG 312
Score = 135 bits (289), Expect(2) = 5e-059
Identities = 64/104 (61%), Positives = 72/104 (69%)
Frame = +3 / -2
Query: 3 HPGVSAASR*SLYKEARKAALTAY*SG*GLPWLSCINPPLVKLNELNTGKYEHMTLSVWL 182
HPGVSAASR*SLY+ A+KAALT Y G*GLPW + I PPLV L + TG+ EHMT+ V L
Sbjct: 611 HPGVSAASR*SLYRVAKKAALTTYWRG*GLPWFN*IKPPLVLLKLVKTGR*EHMTMLV*L 432
Query: 183 WNVLSISS*GYPGXXXXXXXXXXT*APGSLSSQVNTP*FGLPNQ 314
W VL ISS GYPG T*AP SL SQ+NTP* G P +
Sbjct: 431 WKVLRISSYGYPGLKNNLKRLRRT*APASLLSQLNTP*SGPPQK 300
Score = 114 bits (243), Expect(2) = 5e-059
Identities = 56/102 (54%), Positives = 63/102 (61%)
Frame = +3 / -2
Query: 306 PNQYAPACFAIPGAVATVRYELFPVAKYIHAPPHTNSSQ*LVEL*KMAWSDPLGLYRPRC 485
P Q APAC A+PG VA++RYELFPVAKYI APP TNSS+* VEL* +A SD GLY P
Sbjct: 311 PPQKAPACLAMPGVVASLRYELFPVAKYIQAPPQTNSSK*PVEL*NVAASDLPGLYLPL* 132
Query: 486 SLPNLYRALAPXXXXXXXXXXXXNTMEAXXXXAXXXXARSEQ 611
SL NL +AP TM+A A ARSEQ
Sbjct: 131 SLENLKSVVAP*TSFLE*ALLSLKTMDAAAKAAAISAARSEQ 6
Score = 105 bits (224), Expect(2) = 3e-033
Identities = 47/102 (46%), Positives = 70/102 (68%)
Frame = +2 / -1
Query: 2 PPRRVSRFKIVAV*RSKESCVDSVLKRLRPSMVELY*SSFGEVE*IEYR*IRAHDVVGLV 181
P R +S K++ V* S++ C+D++L+RLRP+MV L +SFG V+ E R*+ AHD GL+
Sbjct: 612 PSRSISGIKVITV*GSQKGCIDNILERLRPTMV*LNQASFGSVKACENR*VGAHDDAGLI 433
Query: 182 VECPQYFFVRVPWT*EESQPAEKNLSSWKFVIPGKHSVVWTS 307
VE P FF+ + T E+ Q +KNLS+ K VIP K++++ S
Sbjct: 432 VEGP*NFFIWISRTQEQPQTTKKNLSTSKLVIPAKYTIIRAS 307
Score = 57.9 bits (120), Expect(2) = 3e-033
Identities = 27/87 (31%), Positives = 50/87 (57%)
Frame = +2 / -1
Query: 305 SKPVCSSVLRHSRGCGDGEVRVVPRGEVHPRTAPHELVPVAGGVVEDGLVRSVGAVSPTL 484
+ P +S+L ++ +VRV+ +VHP +P++L+ VAG +VE + VS +L
Sbjct: 312 ASPESTSMLSNAGSSSKLKVRVISGCKVHPGASPNKLIKVAGRIVECCSLGPTWTVSTSL 133
Query: 485 QPAELVQGLGALDELVGVGLAVLEHYG 565
P +L + G +++ +G+G V E+ G
Sbjct: 132 IPGKLEECSGTVNKFLGIGFVVFENNG 52
>gb|AC151000.3| Medicago truncatula chromosome 2 BAC clone mth2-193p1, complete
sequence
Length = 120579
Score = 199 bits (430), Expect(4) = 2e-097
Identities = 80/116 (68%), Positives = 87/116 (75%)
Frame = -2 / +3
Query: 652 EXIDYXXXXXECHSCSDLAXXXXAXXXXASIVFKDSKTYSDKLVKGAKALYKFGRLQRGR 473
E IDY ECHSCSDLA A ASIVFKD+K YS KLV GA L+KF R QRGR
Sbjct: 23283 EDIDYDRPVTECHSCSDLAAEMAAALAAASIVFKDNKAYSKKLVHGATTLFKFSRDQRGR 23462
Query: 472 YSPNGSDQAIFYNSTSYWDEFVWGGAWMYFATGNNSYLTVATAPGMAKHAGAYWFG 305
YSP S+ A FYNST Y+DEFVWGGAWMYFATGNNSYL +AT PG+AKHAGA+W G
Sbjct: 23463 YSPGRSEAATFYNSTGYFDEFVWGGAWMYFATGNNSYLKLATTPGIAKHAGAFWGG 23630
Score = 126 bits (270), Expect(4) = 1e-048
Identities = 62/116 (53%), Positives = 70/116 (60%)
Frame = +3 / -3
Query: 306 PNQYAPACFAIPGAVATVRYELFPVAKYIHAPPHTNSSQ*LVEL*KMAWSDPLGLYRPRC 485
P Q APAC A+PG VA++RYELFPVAKYI APP TNSS+* VEL* +A SD GLY P
Sbjct: 23629 PPQKAPACLAMPGVVASLRYELFPVAKYIQAPPQTNSSK*PVEL*NVAASDLPGLYLPL* 23450
Query: 486 SLPNLYRALAPXXXXXXXXXXXXNTMEAXXXXAXXXXARSEQEWHSXXXXX*SMXS 653
SL NL +AP TM+A A ARSEQ WHS *S+ S
Sbjct: 23449 SLENLKSVVAP*TSFLE*ALLSLKTMDAAAKAAAISAARSEQLWHSVTGLS*SISS 23282
Score = 101 bits (216), Expect(4) = 2e-097
Identities = 42/46 (91%), Positives = 42/46 (91%)
Frame = -2 / +2
Query: 253 VLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSYLPVFNSFNFTKG 116
VLLSRLRLFLSPGYPYEEILRTFHNQT VMCSYLPVF SFN TKG
Sbjct: 24281 VLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPVFTSFNRTKG 24418
Score = 94.1 bits (199), Expect(4) = 2e-097
Identities = 36/39 (92%), Positives = 38/39 (97%)
Frame = -2 / +1
Query: 118 GGLIQLNHGRPQPLQYAVNAAFLASLYSDYLEAADTPGW 2
GGLIQLNHGRPQPLQY VNAAFLA+LYSDYL+AADTPGW
Sbjct: 24544 GGLIQLNHGRPQPLQYVVNAAFLATLYSDYLDAADTPGW 24660
Score = 68.9 bits (144), Expect(4) = 1e-048
Identities = 30/44 (68%), Positives = 34/44 (77%)
Frame = +3 / -2
Query: 3 HPGVSAASR*SLYKEARKAALTAY*SG*GLPWLSCINPPLVKLN 134
HPGVSAASR*SLY+ A+KAALT Y G*GLPW + I PP+ N
Sbjct: 24659 HPGVSAASR*SLYRVAKKAALTTYWRG*GLPWFN*IKPPVCITN 24528
Score = 50.1 bits (103), Expect(4) = 1e-048
Identities = 22/35 (62%), Positives = 25/35 (71%)
Frame = +3 / -1
Query: 117 PLVKLNELNTGKYEHMTLSVWLWNVLSISS*GYPG 221
PLV L + TG+ EHMT+ V LW VL ISS GYPG
Sbjct: 24417 PLVLLKLVKTGR*EHMTMLV*LWKVLRISSYGYPG 24313
Score = 45.1 bits (92), Expect(4) = 2e-097
Identities = 16/25 (64%), Positives = 22/25 (88%)
Frame = -2 / +3
Query: 313 WFGSPNYGVFTWDDKLPGAQVLLSR 239
++G P+YGVF+WD+KL GAQV+L R
Sbjct: 23619 FWGGPDYGVFSWDNKLAGAQVIL*R 23693
Score = 30.9 bits (61), Expect(4) = 1e-048
Identities = 14/21 (66%), Positives = 16/21 (76%)
Frame = +3 / -3
Query: 252 T*APGSLSSQVNTP*FGLPNQ 314
T*AP SL SQ+NTP* G P +
Sbjct: 23680 T*APASLLSQLNTP*SGPPQK 23618
>gb|BI308315.1|BI308315 EST529725 GPOD Medicago truncatula cDNA clone pGPOD-2B18 5' end,
mRNA sequence
Length = 694
Score = 199 bits (430), Expect(3) = 3e-089
Identities = 80/116 (68%), Positives = 87/116 (75%)
Frame = -2 / +1
Query: 652 EXIDYXXXXXECHSCSDLAXXXXAXXXXASIVFKDSKTYSDKLVKGAKALYKFGRLQRGR 473
E IDY ECHSCSDLA A ASIVFKD+K YS KLV GA L+KF R QRGR
Sbjct: 103 EDIDYDRPVTECHSCSDLAAEMAAALAAASIVFKDNKAYSKKLVHGATTLFKFSRDQRGR 282
Query: 472 YSPNGSDQAIFYNSTSYWDEFVWGGAWMYFATGNNSYLTVATAPGMAKHAGAYWFG 305
YSP S+ A FYNST Y+DEFVWGGAWMYFATGNNSYL +AT PG+AKHAGA+W G
Sbjct: 283 YSPGRSEAATFYNSTGYFDEFVWGGAWMYFATGNNSYLKLATTPGIAKHAGAFWGG 450
Score = 142 bits (305), Expect(3) = 3e-089
Identities = 57/69 (82%), Positives = 62/69 (89%)
Frame = -2 / +1
Query: 313 WFGSPNYGVFTWDDKLPGAQVLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSYLPVFNS 134
++G P+YGVF+WD+KL GAQVLLSRLRLFLSPGYPYEEILRTFHNQT VMCSYLPVF S
Sbjct: 439 FWGGPDYGVFSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPVFTS 618
Query: 133 FNFTKGGLI 107
FN TKG LI
Sbjct: 619 FNRTKGSLI 645
Score = 126 bits (270), Expect(2) = 2e-044
Identities = 62/116 (53%), Positives = 70/116 (60%)
Frame = +3 / -3
Query: 306 PNQYAPACFAIPGAVATVRYELFPVAKYIHAPPHTNSSQ*LVEL*KMAWSDPLGLYRPRC 485
P Q APAC A+PG VA++RYELFPVAKYI APP TNSS+* VEL* +A SD GLY P
Sbjct: 449 PPQKAPACLAMPGVVASLRYELFPVAKYIQAPPQTNSSK*PVEL*NVAASDLPGLYLPL* 270
Query: 486 SLPNLYRALAPXXXXXXXXXXXXNTMEAXXXXAXXXXARSEQEWHSXXXXX*SMXS 653
SL NL +AP TM+A A ARSEQ WHS *S+ S
Sbjct: 269 SLENLKSVVAP*TSFLE*ALLSLKTMDAAAKAAAISAARSEQLWHSVTGLS*SISS 102
Score = 73.9 bits (155), Expect(2) = 2e-044
Identities = 37/69 (53%), Positives = 42/69 (60%)
Frame = +3 / -3
Query: 108 INPPLVKLNELNTGKYEHMTLSVWLWNVLSISS*GYPGXXXXXXXXXXT*APGSLSSQVN 287
I PLV L + TG+ EHMT+ V LW VL ISS GYPG T*AP SL SQ+N
Sbjct: 644 IKLPLVLLKLVKTGR*EHMTMLV*LWKVLRISSYGYPGLKNNLKRLRRT*APASLLSQLN 465
Query: 288 TP*FGLPNQ 314
TP* G P +
Sbjct: 464 TP*SGPPQK 438
Score = 65.7 bits (137), Expect(2) = 2e-022
Identities = 30/65 (46%), Positives = 43/65 (66%)
Frame = +2 / -2
Query: 113 SSFGEVE*IEYR*IRAHDVVGLVVECPQYFFVRVPWT*EESQPAEKNLSSWKFVIPGKHS 292
+SFG V+ E R*+ AHD GL+VE P FF+ + T E+ Q +KNLS+ K VIP K++
Sbjct: 639 ASFGSVKACENR*VGAHDDAGLIVEGP*NFFIWISRTQEQPQTTKKNLSTSKLVIPAKYT 460
Query: 293 VVWTS 307
++ S
Sbjct: 459 IIRAS 445
Score = 61.6 bits (128), Expect(2) = 2e-022
Identities = 33/116 (28%), Positives = 59/116 (50%)
Frame = +2 / -2
Query: 305 SKPVCSSVLRHSRGCGDGEVRVVPRGEVHPRTAPHELVPVAGGVVEDGLVRSVGAVSPTL 484
+ P +S+L ++ +VRV+ +VHP +P++L+ VAG +VE + VS +L
Sbjct: 450 ASPESTSMLSNAGSSSKLKVRVISGCKVHPGASPNKLIKVAGRIVECCSLGPTWTVSTSL 271
Query: 485 QPAELVQGLGALDELVGVGLAVLEHYGSXXXXXXXXXR*I*AGVALXXXXXVVDVL 652
P +L + G +++ +G+G V E+ G I* VA +V++L
Sbjct: 270 IPGKLEECSGTVNKFLGIGFVVFENNGCSSQGSGHFRSKI*TTVAFSDWPVIVNIL 103
Score = 27.2 bits (53), Expect(3) = 3e-089
Identities = 10/12 (83%), Positives = 10/12 (83%)
Frame = -2 / +3
Query: 112 LIQLNHGRPQPL 77
L LNHGRPQPL
Sbjct: 639 LDSLNHGRPQPL 674
>emb|CT030180.3| M.truncatula DNA sequence from clone MTH2-177C12 on chromosome 3,
complete sequence
Length = 141577
Score = 95.0 bits (201), Expect(4) = 1e-061
Identities = 35/69 (50%), Positives = 49/69 (71%)
Frame = -2 / +1
Query: 313 WFGSPNYGVFTWDDKLPGAQVLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSYLPVFNS 134
++ P+ V +WD+KLP A +LL+R+R+FL+PGYPYE++L +HN T MCSYL FN
Sbjct: 135172 FYMKPDLSVLSWDNKLPAAMLLLTRVRMFLNPGYPYEDMLSMYHNVTSLTMCSYLHQFNV 135351
Query: 133 FNFTKGGLI 107
F +T G I
Sbjct: 135352 FKWTNGNSI 135378
Score = 79.4 bits (167), Expect(4) = 1e-061
Identities = 30/39 (76%), Positives = 34/39 (87%)
Frame = -2 / +2
Query: 118 GGLIQLNHGRPQPLQYAVNAAFLASLYSDYLEAADTPGW 2
GGLIQLNHG+PQ LQY VNAAFLASL++DY+EA PGW
Sbjct: 135476 GGLIQLNHGQPQSLQYVVNAAFLASLFADYMEAKGVPGW 135592
Score = 76.7 bits (161), Expect(4) = 1e-061
Identities = 27/58 (46%), Positives = 42/58 (72%)
Frame = -2 / +1
Query: 484 QRGRYSPNGSDQAIFYNSTSYWDEFVWGGAWMYFATGNNSYLTVATAPGMAKHAGAYW 311
+R YS FYNS+ Y+DE++WGGAW+YFATGN +Y+++AT P + K++ A++
Sbjct: 135004 KRSTYSRGKPYIEPFYNSSGYFDEYMWGGAWLYFATGNTTYISLATNPDVPKNSKAFY 135177
Score = 76.7 bits (161), Expect(4) = 2e-021
Identities = 36/89 (40%), Positives = 50/89 (56%)
Frame = -2 / +3
Query: 403 GGAWMYFATGNNSYLTVATAPGMAKHAGAYWFGSPNYGVFTWDDKLPGAQVLLSRLRLFL 224
GG +YFATGNN+YL++AT P + K + P +W+ KLP A +LL+++ F
Sbjct: 121488 GGV*LYFATGNNNYLSLATDPSIYKINDKAFNEIPYLKFLSWNIKLPAAMLLLTKMMRFH 121667
Query: 223 SPGYPYEEILRTFHNQTDNVMCSYLPVFN 137
GYP EE+L +H SYL FN
Sbjct: 121668 KFGYPNEEMLSMYHISIGLTTGSYLHQFN 121754
Score = 52.4 bits (108), Expect(3) = 3e-021
Identities = 21/40 (52%), Positives = 26/40 (65%)
Frame = +3 / -2
Query: 3 HPGVSAASR*SLYKEARKAALTAY*SG*GLPWLSCINPPL 122
HPG AS *S ++A+ AALT Y G PW +CINPP+
Sbjct: 135591 HPGTPLASI*SAKRDAKNAALTTYWRDCGCPWFNCINPPV 135472
Score = 49.6 bits (102), Expect(3) = 3e-021
Identities = 28/61 (45%), Positives = 32/61 (52%)
Frame = +3 / -3
Query: 108 INPPLVKLNELNTGKYEHMTLSVWLWNVLSISS*GYPGXXXXXXXXXXT*APGSLSSQVN 287
I PLV L LN + EHM V LW +LSISS*G PG A G+L SQ+
Sbjct: 135377 IELPLVHLKTLN**R*EHMVRLVTLWYILSISS*G*PGLRNILTLVSSNIAAGNLLSQLK 135198
Query: 288 T 290
T
Sbjct: 135197 T 135195
Score = 44.1 bits (90), Expect(4) = 1e-061
Identities = 20/39 (51%), Positives = 22/39 (56%)
Frame = -2 / +1
Query: 604 DLAXXXXAXXXXASIVFKDSKTYSDKLVKGAKALYKFGR 488
DLA A ASIVF D YS KLVKGA+ + F R
Sbjct: 134878 DLAGEMAAALAAASIVFLDDAAYSKKLVKGAETAFAFAR 134994
Score = 44.1 bits (90), Expect(4) = 2e-021
Identities = 17/28 (60%), Positives = 22/28 (78%)
Frame = -2 / +2
Query: 118 GGLIQLNHGRPQPLQYAVNAAFLASLYS 35
GGL+Q NH +P+ LQY VN AFLA L++
Sbjct: 122057 GGLMQFNHRQPKSLQYFVNVAFLAYLFA 122140
Score = 38.2 bits (77), Expect(3) = 3e-021
Identities = 20/34 (58%), Positives = 22/34 (64%)
Frame = +3 / -3
Query: 342 GAVATVRYELFPVAKYIHAPPHTNSSQ*LVEL*K 443
G VA +FPVAKY APPH SS+* EL*K
Sbjct: 135146 GLVANEM*VVFPVAKYNQAPPHMYSSK*PDEL*K 135045
Score = 33.6 bits (67), Expect(4) = 2e-021
Identities = 10/20 (50%), Positives = 15/20 (75%)
Frame = -2 / +2
Query: 442 FYNSTSYWDEFVWGGAWMYF 383
+YNST Y+DE++WG + F
Sbjct: 121448 YYNSTDYFDEYMWGRCLIVF 121507
Score = 29.0 bits (57), Expect(4) = 2e-021
Identities = 11/25 (44%), Positives = 16/25 (64%)
Frame = -2 / +2
Query: 562 IVFKDSKTYSDKLVKGAKALYKFGR 488
I+F+ YS KL+KG +AL+ R
Sbjct: 121322 IIFEHDIAYSKKLIKGDEALFALAR 121396
>gb|AC144618.7| Medicago truncatula clone mth2-21d12, complete sequence
Length = 106554
Score = 95.0 bits (201), Expect(4) = 1e-061
Identities = 35/69 (50%), Positives = 49/69 (71%)
Frame = -2 / -3
Query: 313 WFGSPNYGVFTWDDKLPGAQVLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSYLPVFNS 134
++ P+ V +WD+KLP A +LL+R+R+FL+PGYPYE++L +HN T MCSYL FN
Sbjct: 91435 FYMKPDLSVLSWDNKLPAAMLLLTRVRMFLNPGYPYEDMLSMYHNVTSLTMCSYLHQFNV 91256
Query: 133 FNFTKGGLI 107
F +T G I
Sbjct: 91255 FKWTNGNSI 91229
Score = 79.4 bits (167), Expect(4) = 1e-061
Identities = 30/39 (76%), Positives = 34/39 (87%)
Frame = -2 / -1
Query: 118 GGLIQLNHGRPQPLQYAVNAAFLASLYSDYLEAADTPGW 2
GGLIQLNHG+PQ LQY VNAAFLASL++DY+EA PGW
Sbjct: 91131 GGLIQLNHGQPQSLQYVVNAAFLASLFADYMEAKGVPGW 91015
Score = 76.7 bits (161), Expect(4) = 1e-061
Identities = 27/58 (46%), Positives = 42/58 (72%)
Frame = -2 / -3
Query: 484 QRGRYSPNGSDQAIFYNSTSYWDEFVWGGAWMYFATGNNSYLTVATAPGMAKHAGAYW 311
+R YS FYNS+ Y+DE++WGGAW+YFATGN +Y+++AT P + K++ A++
Sbjct: 91603 KRSTYSRGKPYIEPFYNSSGYFDEYMWGGAWLYFATGNTTYISLATNPDVPKNSKAFY 91430
Score = 76.7 bits (161), Expect(4) = 9e-022
Identities = 36/89 (40%), Positives = 50/89 (56%)
Frame = -2 / -2
Query: 403 GGAWMYFATGNNSYLTVATAPGMAKHAGAYWFGSPNYGVFTWDDKLPGAQVLLSRLRLFL 224
GG +YFATGNN+YL++AT P + K + P +W+ KLP A +LL+++ F
Sbjct: 105119 GGV*LYFATGNNNYLSLATDPSIYKINDKAFNEIPYLKFLSWNIKLPAAMLLLTKMMRFH 104940
Query: 223 SPGYPYEEILRTFHNQTDNVMCSYLPVFN 137
GYP EE+L +H SYL FN
Sbjct: 104939 KFGYPNEEMLSMYHISIGLTTGSYLHQFN 104853
Score = 52.4 bits (108), Expect(3) = 3e-021
Identities = 21/40 (52%), Positives = 26/40 (65%)
Frame = +3 / +2
Query: 3 HPGVSAASR*SLYKEARKAALTAY*SG*GLPWLSCINPPL 122
HPG AS *S ++A+ AALT Y G PW +CINPP+
Sbjct: 91016 HPGTPLASI*SAKRDAKNAALTTYWRDCGCPWFNCINPPV 91135
Score = 49.6 bits (102), Expect(3) = 3e-021
Identities = 28/61 (45%), Positives = 32/61 (52%)
Frame = +3 / +3
Query: 108 INPPLVKLNELNTGKYEHMTLSVWLWNVLSISS*GYPGXXXXXXXXXXT*APGSLSSQVN 287
I PLV L LN + EHM V LW +LSISS*G PG A G+L SQ+
Sbjct: 91230 IELPLVHLKTLN**R*EHMVRLVTLWYILSISS*G*PGLRNILTLVSSNIAAGNLLSQLK 91409
Query: 288 T 290
T
Sbjct: 91410 T 91412
Score = 44.1 bits (90), Expect(4) = 1e-061
Identities = 20/39 (51%), Positives = 22/39 (56%)
Frame = -2 / -3
Query: 604 DLAXXXXAXXXXASIVFKDSKTYSDKLVKGAKALYKFGR 488
DLA A ASIVF D YS KLVKGA+ + F R
Sbjct: 91729 DLAGEMAAALAAASIVFLDDAAYSKKLVKGAETAFAFAR 91613
Score = 44.1 bits (90), Expect(4) = 9e-022
Identities = 17/28 (60%), Positives = 22/28 (78%)
Frame = -2 / -1
Query: 118 GGLIQLNHGRPQPLQYAVNAAFLASLYS 35
GGL+Q NH +P+ LQY VN AFLA L++
Sbjct: 104550 GGLMQFNHRQPKSLQYFVNVAFLAYLFA 104467
Score = 38.2 bits (77), Expect(3) = 3e-021
Identities = 20/34 (58%), Positives = 22/34 (64%)
Frame = +3 / +3
Query: 342 GAVATVRYELFPVAKYIHAPPHTNSSQ*LVEL*K 443
G VA +FPVAKY APPH SS+* EL*K
Sbjct: 91461 GLVANEM*VVFPVAKYNQAPPHMYSSK*PDEL*K 91562
Score = 33.6 bits (67), Expect(4) = 9e-022
Identities = 10/20 (50%), Positives = 15/20 (75%)
Frame = -2 / -1
Query: 442 FYNSTSYWDEFVWGGAWMYF 383
+YNST Y+DE++WG + F
Sbjct: 105159 YYNSTDYFDEYMWGRCLIVF 105100
Score = 29.0 bits (57), Expect(4) = 9e-022
Identities = 11/25 (44%), Positives = 16/25 (64%)
Frame = -2 / -1
Query: 562 IVFKDSKTYSDKLVKGAKALYKFGR 488
I+F+ YS KL+KG +AL+ R
Sbjct: 105285 IIFEHDIAYSKKLIKGDEALFALAR 105211
>gb|BI270588.1|BI270588 NF010B12FL1F1101 Developing flower Medicago truncatula cDNA clone
NF010B12FL 5', mRNA sequence
Length = 650
Score = 199 bits (430), Expect(2) = 3e-053
Identities = 80/116 (68%), Positives = 87/116 (75%)
Frame = -2 / +2
Query: 652 EXIDYXXXXXECHSCSDLAXXXXAXXXXASIVFKDSKTYSDKLVKGAKALYKFGRLQRGR 473
E IDY ECHSCSDLA A ASIVFKD+K YS KLV GA L+KF R QRGR
Sbjct: 254 EDIDYDRPVTECHSCSDLAAEMAAALAAASIVFKDNKAYSKKLVHGATTLFKFSRDQRGR 433
Query: 472 YSPNGSDQAIFYNSTSYWDEFVWGGAWMYFATGNNSYLTVATAPGMAKHAGAYWFG 305
YSP S+ A FYNST Y+DEFVWGGAWMYFATGNNSYL +AT PG+AKHAGA+W G
Sbjct: 434 YSPGRSEAATFYNSTGYFDEFVWGGAWMYFATGNNSYLKLATTPGIAKHAGAFWGG 601
Score = 126 bits (270), Expect = 3e-028
Identities = 62/116 (53%), Positives = 70/116 (60%)
Frame = +3 / -3
Query: 306 PNQYAPACFAIPGAVATVRYELFPVAKYIHAPPHTNSSQ*LVEL*KMAWSDPLGLYRPRC 485
P Q APAC A+PG VA++RYELFPVAKYI APP TNSS+* VEL* +A SD GLY P
Sbjct: 600 PPQKAPACLAMPGVVASLRYELFPVAKYIQAPPQTNSSK*PVEL*NVAASDLPGLYLPL* 421
Query: 486 SLPNLYRALAPXXXXXXXXXXXXNTMEAXXXXAXXXXARSEQEWHSXXXXX*SMXS 653
SL NL +AP TM+A A ARSEQ WHS *S+ S
Sbjct: 420 SLENLKSVVAP*TSFLE*ALLSLKTMDAAAKAAAISAARSEQLWHSVTGLS*SISS 253
Score = 30.4 bits (60), Expect(2) = 3e-053
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = -2 / +2
Query: 313 WFGSPNYGVFTWDDKL 266
++G P+YGVF+WD L
Sbjct: 590 FWGGPDYGVFSWDTSL 637
>gb|AC135102.16| Medicago truncatula clone mth2-8m3, complete sequence
Length = 144042
Score = 167 bits (359), Expect = 2e-040
Identities = 68/98 (69%), Positives = 72/98 (73%)
Frame = -2 / -3
Query: 652 EXIDYXXXXXECHSCSDLAXXXXAXXXXASIVFKDSKTYSDKLVKGAKALYKFGRLQRGR 473
E IDY ECHSCSDLA A ASIVFKD+K YS KLV GA L+KF R QRGR
Sbjct: 295 EDIDYDRPVTECHSCSDLAAEMAAALAAASIVFKDNKAYSKKLVHGATTLFKFSRDQRGR 116
Query: 472 YSPNGSDQAIFYNSTSYWDEFVWGGAWMYFATGNNSYL 359
YSP S+ A FYNST Y+DEFVWGGAWMYFATGNNSYL
Sbjct: 115 YSPGRSEAATFYNSTGYFDEFVWGGAWMYFATGNNSYL 2
>gb|CG936178.1|CG936178 MBENI74TR mth2 Medicago truncatula genomic clone 8M3, DNA sequence
Length = 845
Score = 152 bits (326), Expect = 6e-036
Identities = 62/92 (67%), Positives = 66/92 (71%)
Frame = -2 / -3
Query: 652 EXIDYXXXXXECHSCSDLAXXXXAXXXXASIVFKDSKTYSDKLVKGAKALYKFGRLQRGR 473
E IDY ECHSCSDLA A ASIVFKD+K YS KLV GA L+KF R QRGR
Sbjct: 279 EDIDYDRPVTECHSCSDLAAEMAAALAAASIVFKDNKAYSKKLVHGATTLFKFSRDQRGR 100
Query: 472 YSPNGSDQAIFYNSTSYWDEFVWGGAWMYFAT 377
YSP S+ A FYNST Y+DEFVWGGAWMYFAT
Sbjct: 99 YSPGRSEAATFYNSTGYFDEFVWGGAWMYFAT 4
>gb|AY308955.1| Medicago truncatula cellulase mRNA, complete cds
Length = 2074
Score = 65.7 bits (137), Expect(4) = 9e-034
Identities = 24/48 (50%), Positives = 34/48 (70%)
Frame = -2 / +2
Query: 292 GVFTWDDKLPGAQVLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSYL 149
GVF W++KL VLL+ +R F PG+PYE++L+ N T ++MCSYL
Sbjct: 1202 GVFYWNNKLSAVAVLLTGIRYFRDPGFPYEDVLKFSSNSTHSLMCSYL 1345
Score = 52.8 bits (109), Expect(4) = 9e-034
Identities = 19/38 (50%), Positives = 22/38 (57%)
Frame = -2 / +2
Query: 460 GSDQAIFYNSTSYWDEFVWGGAWMYFATGNNSYLTVAT 347
G + YNSTSY DE WG W++ AT N YL AT
Sbjct: 1043 GKQARMLYNSTSYKDELAWGATWLFLATKNTDYLANAT 1156
Score = 42.3 bits (86), Expect(4) = 9e-034
Identities = 18/39 (46%), Positives = 26/39 (66%)
Frame = -2 / +2
Query: 613 SCSDLAXXXXAXXXXASIVFKDSKTYSDKLVKGAKALYK 497
S +DLA A AS+VF++ K YS KLV+ A++LY+
Sbjct: 869 SATDLAGEIVAALSAASMVFEEDKEYSGKLVQAAESLYE 985
Score = 39.6 bits (80), Expect(4) = 9e-034
Identities = 17/34 (50%), Positives = 22/34 (64%)
Frame = -2 / +2
Query: 124 TKGGLIQLNHGRPQPLQYAVNAAFLASLYSDYLE 23
T GGL+ LQYAV A+FL+ LYSDY++
Sbjct: 1367 TPGGLVIPKPDNGPLLQYAVTASFLSKLYSDYID 1468
>gb|BQ165567.1|BQ165567 EST611436 KVKC Medicago truncatula cDNA clone pKVKC-10B7, mRNA
sequence
Length = 798
Score = 65.7 bits (137), Expect(4) = 9e-034
Identities = 24/48 (50%), Positives = 34/48 (70%)
Frame = -2 / +2
Query: 292 GVFTWDDKLPGAQVLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSYL 149
GVF W++KL VLL+ +R F PG+PYE++L+ N T ++MCSYL
Sbjct: 536 GVFYWNNKLSAVAVLLTGIRYFRDPGFPYEDVLKFSSNSTHSLMCSYL 679
Score = 52.8 bits (109), Expect(4) = 9e-034
Identities = 19/38 (50%), Positives = 22/38 (57%)
Frame = -2 / +2
Query: 460 GSDQAIFYNSTSYWDEFVWGGAWMYFATGNNSYLTVAT 347
G + YNSTSY DE WG W++ AT N YL AT
Sbjct: 377 GKQARMLYNSTSYKDELAWGATWLFLATKNTDYLANAT 490
Score = 44.1 bits (90), Expect(4) = 9e-034
Identities = 19/39 (48%), Positives = 26/39 (66%)
Frame = -2 / +2
Query: 613 SCSDLAXXXXAXXXXASIVFKDSKTYSDKLVKGAKALYK 497
S +DLA A AS+VFK+ K YS KLV+ A++LY+
Sbjct: 203 SATDLAGEIVAALSAASMVFKEDKEYSGKLVQAAESLYE 319
Score = 37.7 bits (76), Expect(4) = 9e-034
Identities = 17/32 (53%), Positives = 20/32 (62%)
Frame = -2 / +2
Query: 124 TKGGLIQLNHGRPQPLQYAVNAAFLASLYSDY 29
T GGL+ LQYAV A+FL+ LYSDY
Sbjct: 701 TPGGLVIPKPDNGPLLQYAVTASFLSKLYSDY 796
>gb|AJ845540.1|AJ845540 AJ845540 MtSNF Medicago truncatula cDNA clone MtNF06M12N1, mRNA
sequence
Length = 295
Score = 134 bits (288), Expect = 1e-030
Identities = 56/86 (65%), Positives = 60/86 (69%)
Frame = -2 / -1
Query: 652 EXIDYXXXXXECHSCSDLAXXXXAXXXXASIVFKDSKTYSDKLVKGAKALYKFGRLQRGR 473
E IDY ECHSCSDLA A ASIVFKD+K YS KLV GA L+KF R QRGR
Sbjct: 259 EDIDYDRPVTECHSCSDLAAEMAAALAAASIVFKDNKAYSKKLVHGATTLFKFSRDQRGR 80
Query: 472 YSPNGSDQAIFYNSTSYWDEFVWGGA 395
YSP S+ A FYNST Y+DEFVWGGA
Sbjct: 79 YSPGRSEAATFYNSTGYFDEFVWGGA 2
>gb|AJ845845.1|AJ845845 AJ845845 MtSNF Medicago truncatula cDNA clone MtNF11L17N1, mRNA
sequence
Length = 298
Score = 112 bits (239), Expect(2) = 3e-024
Identities = 48/77 (62%), Positives = 51/77 (66%)
Frame = -2 / -2
Query: 652 EXIDYXXXXXECHSCSDLAXXXXAXXXXASIVFKDSKTYSDKLVKGAKALYKFGRLQRGR 473
E IDY ECHSCSDLA A ASIVFKD+K YS KLV GA L+KF R QRGR
Sbjct: 258 EDIDYDRPVTECHSCSDLAAEMAAALAAASIVFKDNKAYSKKLVHGATTLFKFSRDQRGR 79
Query: 472 YSPNGSDQAIFYNSTSY 422
YSP S+ A FYNST Y
Sbjct: 78 YSPGRSEAATFYNSTGY 28
Score = 19.9 bits (37), Expect(2) = 3e-024
Identities = 6/6 (100%), Positives = 6/6 (100%)
Frame = -2 / -3
Query: 412 FVWGGA 395
FVWGGA
Sbjct: 20 FVWGGA 3
>gb|AC150784.20| Medicago truncatula clone mth2-134j11, WORKING DRAFT SEQUENCE
Length = 116850
Score = 52.8 bits (109), Expect(4) = 4e-023
Identities = 19/38 (50%), Positives = 22/38 (57%)
Frame = -2 / +2
Query: 460 GSDQAIFYNSTSYWDEFVWGGAWMYFATGNNSYLTVAT 347
G + YNSTSY DE WG W++ AT N YL AT
Sbjct: 34586 GKQARMLYNSTSYKDELAWGATWLFLATKNTDYLANAT 34699
Score = 52.4 bits (108), Expect(4) = 4e-023
Identities = 19/36 (52%), Positives = 27/36 (75%)
Frame = -2 / +1
Query: 256 QVLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSYL 149
QVLL+ +R F PG+PYE++L+ N T ++MCSYL
Sbjct: 34870 QVLLTGIRYFRDPGFPYEDVLKFSSNSTHSLMCSYL 34977
Score = 44.1 bits (90), Expect(4) = 4e-023
Identities = 19/39 (48%), Positives = 26/39 (66%)
Frame = -2 / +2
Query: 613 SCSDLAXXXXAXXXXASIVFKDSKTYSDKLVKGAKALYK 497
S +DLA A AS+VFK+ K YS KLV+ A++LY+
Sbjct: 34412 SATDLAGEIVAALSAASMVFKEDKEYSGKLVQAAESLYE 34528
Score = 39.1 bits (79), Expect(4) = 4e-023
Identities = 17/36 (47%), Positives = 22/36 (61%)
Frame = -2 / +2
Query: 130 NFTKGGLIQLNHGRPQPLQYAVNAAFLASLYSDYLE 23
N GGL+ LQYAV A+FL+ LYSDY++
Sbjct: 35105 NIIVGGLVIPKPDNGPLLQYAVTASFLSKLYSDYID 35212
>gb|AC127169.13| Medicago truncatula clone mth2-36n3, complete sequence
Length = 124554
Score = 69.8 bits (146), Expect(3) = 2e-022
Identities = 25/39 (64%), Positives = 31/39 (79%)
Frame = -2 / +2
Query: 118 GGLIQLNHGRPQPLQYAVNAAFLASLYSDYLEAADTPGW 2
GG IQLNHG P LQY VNA+F+ASL++DY++A PGW
Sbjct: 101651 GGFIQLNHGYPNSLQYVVNASFMASLFADYMKAQGVPGW 101767
Score = 53.3 bits (110), Expect(3) = 2e-022
Identities = 16/33 (48%), Positives = 26/33 (78%)
Frame = -2 / +3
Query: 442 FYNSTSYWDEFVWGGAWMYFATGNNSYLTVATA 344
+ NS+ Y+DE++WG W++ AT NN+YL++A A
Sbjct: 101211 YNNSSGYFDEYMWGSVWLFLATSNNTYLSLAMA 101309
Score = 39.1 bits (79), Expect(3) = 2e-022
Identities = 15/26 (57%), Positives = 16/26 (61%)
Frame = -2 / +2
Query: 232 LFLSPGYPYEEILRTFHNQTDNVMCS 155
L L GYPYEEIL +H T MCS
Sbjct: 101402 LILVSGYPYEEILSMYHESTSLTMCS 101479
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 526,032,717
Number of Sequences: 392609
Number of extensions: 6470866
Number of successful extensions: 32546
Number of sequences better than 1.0e-020: 16
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 22057602875
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)