TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF18e02.yg.3.1
(315 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CX535064.1|CX535064 s13dNF36E10MJ083_388309 Methyl Jasmo... 87 1e-016
gb|AC151481.4| Medicago truncatula clone mth2-3m11, complet... 80 1e-014
gb|AC148487.14| Medicago truncatula clone mth2-57f20, compl... 37 0.11
gb|AC162162.15| Medicago truncatula clone mth2-71a20, WORKI... 37 0.11
emb|CR480710.1| mth2-192H22RM1 BAC end, cultivar Jemalong A... 33 2.0
gb|AC172743.2| Medicago truncatula chromosome 2 clone mth2-... 33 2.0
gb|AC131239.20| Medicago truncatula clone mth2-18o15, compl... 32 3.7
gb|AC157538.18| Medicago truncatula clone mth2-150a17, WORK... 32 3.7
gb|AW736675.1|AW736675 EST333167 KV3 Medicago truncatula cD... 28 4.1
gb|AW736676.1|AW736676 EST333168 KV3 Medicago truncatula cD... 28 4.6
gb|BE248522.2|BE248522 NF006F07DT1F1060 Drought Medicago tr... 31 5.1
gb|BQ137613.1|BQ137613 NF029A08NR1F1000 Nodulated root Medi... 27 5.5
gb|AC152177.23| Medicago truncatula clone mth2-65h3, WORKIN... 29 6.6
gb|AJ389047.1|AJ389047 AJ389047 Medicago truncatula R108 Me... 31 7.0
gb|BG647956.1|BG647956 EST509575 HOGA Medicago truncatula c... 31 7.0
gb|DW017929.1|DW017929 EST1226890 MTY Medicago truncatula c... 31 7.0
gb|AC150779.1| Medicago truncatula chromosome 2 clone mth2-... 31 7.0
emb|CT009553.4| M.truncatula DNA sequence from clone MTH2-1... 31 7.0
gb|BE325816.1|BE325816 NF020C02ST1F1007 Developing stem Med... 30 9.6
gb|BF636183.1|BF636183 NF106E12DT1F1097 Drought Medicago tr... 30 9.6
gb|CX536396.1|CX536396 s13dNF22D05GS045_455966 Germinating ... 30 9.6
gb|AC121237.19| Medicago truncatula clone mth2-22g11, compl... 30 9.6
gb|AC130804.44| Medicago truncatula clone mth2-15l5, WORKIN... 30 9.6
gb|AC174345.1| Medicago truncatula clone mth2-167k9, WORKIN... 30 9.6
gb|AC174345.5| Medicago truncatula clone mth2-167k9, WORKIN... 30 9.6
gb|AC174294.6| Medicago truncatula clone mth2-71d8, WORKING... 30 9.6
>gb|CX535064.1|CX535064 s13dNF36E10MJ083_388309 Methyl Jasmonate-Elicited Root Cell
Suspension Culture Medicago truncatula cDNA, mRNA
sequence
Length = 558
Score = 86.8 bits (183), Expect = 1e-016
Identities = 36/61 (59%), Positives = 44/61 (72%)
Frame = +3 / +1
Query: 132 ITPNKGSILSKIISVTVRTIQKDAEEKKASFNPRPYFRLXINWLYDLTTTDGHHDGSNFQ 311
I +LSKI++VTVR I KDAEEKK SFNPRP+FRL INWL DL + + DG+N Q
Sbjct: 67 ILKGSSKLLSKILAVTVRFIVKDAEEKKVSFNPRPFFRLFINWLLDLGSLEPVTDGANLQ 246
Query: 312 V 314
+
Sbjct: 247 I 249
Score = 46.0 bits (94), Expect = 2e-004
Identities = 23/53 (43%), Positives = 31/53 (58%)
Frame = -2 / -1
Query: 314 NLKIRTIMMAISSSKVIEPIYXQSEVWSGIK*SFLFLSIFLNGPHSNRNNLGK 156
NL+I TI + +++ +PI QSE WS IK + L L IF N N NLG+
Sbjct: 249 NLQICTISNRLK*AEIQKPIDEQSEKWSWIKRNLLLLCIFYNESDGNCQNLGE 91
Score = 44.6 bits (91), Expect = 5e-004
Identities = 23/37 (62%), Positives = 24/37 (64%)
Frame = -3 / -2
Query: 259 QFIXNRKYGRGLNEAFFSSASF*MVLTVTEIILESIL 149
Q + N K G GL E FFSSASF M LTVT ILE L
Sbjct: 194 QLMNNLKNGLGLKETFFSSASFTMNLTVTARILERSL 84
Score = 31.8 bits (63), Expect = 3.7
Identities = 13/22 (59%), Positives = 15/22 (68%)
Frame = +1 / +2
Query: 196 KMLRKRKLHLIPDHTSDCX*IG 261
KM R+R+ LI DH SDC IG
Sbjct: 131 KMQRRRRFLLIQDHFSDCSSIG 196
>gb|AC151481.4| Medicago truncatula clone mth2-3m11, complete sequence
Length = 99012
Score = 79.9 bits (168), Expect = 1e-014
Identities = 33/51 (64%), Positives = 40/51 (78%)
Frame = +3 / +1
Query: 162 KIISVTVRTIQKDAEEKKASFNPRPYFRLXINWLYDLTTTDGHHDGSNFQV 314
+I++VTVR I KDAEEKK SFNPRP+FRL INWL DL + + DG+N QV
Sbjct: 47830 QILAVTVRFIVKDAEEKKVSFNPRPFFRLFINWLLDLGSLEPVTDGANLQV 47982
Score = 42.8 bits (87), Expect = 0.002
Identities = 23/46 (50%), Positives = 26/46 (56%)
Frame = -3 / -2
Query: 259 QFIXNRKYGRGLNEAFFSSASF*MVLTVTEIILESILPLFGVISTE 122
Q + N K G GL E FFSSASF M LTVT I + + F TE
Sbjct: 47927 QLMNNLKNGLGLKETFFSSASFTMNLTVTARICQKAIYSF*TTITE 47790
Score = 40.5 bits (82), Expect = 0.009
Identities = 21/51 (41%), Positives = 29/51 (56%)
Frame = -2 / -1
Query: 314 NLKIRTIMMAISSSKVIEPIYXQSEVWSGIK*SFLFLSIFLNGPHSNRNNL 162
+L+I TI + +++ +PI QSE WS IK + L L IF N N NL
Sbjct: 47982 HLQICTISNRLK*AEIQKPIDEQSEKWSWIKRNLLLLCIFYNESDGNCQNL 47830
Score = 31.8 bits (63), Expect = 3.7
Identities = 13/22 (59%), Positives = 15/22 (68%)
Frame = +1 / +2
Query: 196 KMLRKRKLHLIPDHTSDCX*IG 261
KM R+R+ LI DH SDC IG
Sbjct: 47864 KMQRRRRFLLIQDHFSDCSSIG 47929
>gb|AC148487.14| Medicago truncatula clone mth2-57f20, complete sequence
Length = 119671
Score = 36.8 bits (74), Expect = 0.11
Identities = 11/20 (55%), Positives = 16/20 (80%)
Frame = +1 / +2
Query: 25 LVDHLSNHHSNPIFHIFPLI 84
L+ + NHHSNP+FH+F L+
Sbjct: 51584 LISPVINHHSNPLFHVFDLV 51643
>gb|AC162162.15| Medicago truncatula clone mth2-71a20, WORKING DRAFT SEQUENCE, 9
unordered pieces
Length = 132597
Score = 36.8 bits (74), Expect = 0.11
Identities = 11/20 (55%), Positives = 16/20 (80%)
Frame = +1 / +2
Query: 25 LVDHLSNHHSNPIFHIFPLI 84
L+ + NHHSNP+FH+F L+
Sbjct: 38318 LISPVINHHSNPLFHVFDLV 38377
>emb|CR480710.1| mth2-192H22RM1 BAC end, cultivar Jemalong A17 of Medicago
truncatula, genomic survey sequence
Length = 539
Score = 32.7 bits (65), Expect = 2.0
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = -2 / -3
Query: 164 LGKYTAFIWCYLH*RVLENHNHELC 90
LG + +CY+H +L NH +LC
Sbjct: 465 LGSFLCLFYCYIHWSILNNHICDLC 391
>gb|AC172743.2| Medicago truncatula chromosome 2 clone mth2-26c15, *** SEQUENCING IN
PROGRESS ***, 2 ordered pieces
Length = 137465
Score = 32.7 bits (65), Expect = 2.0
Identities = 15/35 (42%), Positives = 21/35 (60%)
Frame = -2 / +1
Query: 215 FLFLSIFLNGPHSNRNNLGKYTAFIWCYLH*RVLE 111
FL+L IFLN +S ++ L +Y F + LH LE
Sbjct: 104749 FLYLFIFLNFLNSKQDGLARYFLFFFLNLHTHSLE 104853
>gb|AC131239.20| Medicago truncatula clone mth2-18o15, complete sequence
Length = 140751
Score = 31.8 bits (63), Expect = 3.7
Identities = 15/44 (34%), Positives = 22/44 (50%)
Frame = -3 / +1
Query: 190 MVLTVTEIILESILPLFGVISTEEYLRTITTSFAYESMEKYEIW 59
+VL + EI+ E +LP FGVI L I + ++K W
Sbjct: 98479 LVLHLVEIVEEEVLPYFGVIRLTATLLIIQLTILVLKLKKSVAW 98610
>gb|AC157538.18| Medicago truncatula clone mth2-150a17, WORKING DRAFT SEQUENCE, 2
ordered pieces
Length = 102509
Score = 31.8 bits (63), Expect = 3.7
Identities = 15/44 (34%), Positives = 22/44 (50%)
Frame = -3 / +2
Query: 190 MVLTVTEIILESILPLFGVISTEEYLRTITTSFAYESMEKYEIW 59
+VL + EI+ E +LP FGVI L I + ++K W
Sbjct: 91586 LVLHLVEIVEEEVLPYFGVIRLTATLLIIQLTILVLKLKKSVAW 91717
>gb|AW736675.1|AW736675 EST333167 KV3 Medicago truncatula cDNA clone pKV3-21K13, mRNA
sequence
Length = 481
Score = 27.6 bits (54), Expect(2) = 4.1
Identities = 10/32 (31%), Positives = 17/32 (53%)
Frame = +3 / -3
Query: 147 GSILSKIISVTVRTIQKDAEEKKASFNPRPYF 242
G +LS + ++ ++ DA K F P P+F
Sbjct: 164 GELLSAFLDCSLLPMKPDAHFSKLEFQPLPFF 69
Score = 19.9 bits (37), Expect(2) = 4.1
Identities = 5/8 (62%), Positives = 7/8 (87%)
Frame = +1 / -1
Query: 64 FHIFPLIH 87
FH+ PL+H
Sbjct: 193 FHVVPLLH 170
>gb|AW736676.1|AW736676 EST333168 KV3 Medicago truncatula cDNA clone pKV3-21K13, mRNA
sequence
Length = 554
Score = 27.6 bits (54), Expect(2) = 4.6
Identities = 10/32 (31%), Positives = 17/32 (53%)
Frame = +3 / -2
Query: 147 GSILSKIISVTVRTIQKDAEEKKASFNPRPYF 242
G +LS + ++ ++ DA K F P P+F
Sbjct: 160 GELLSAFLDCSLLPMKPDAHFSKLEFQPLPFF 65
Score = 19.9 bits (37), Expect(2) = 4.6
Identities = 5/8 (62%), Positives = 7/8 (87%)
Frame = +1 / -3
Query: 64 FHIFPLIH 87
FH+ PL+H
Sbjct: 189 FHVVPLLH 166
>gb|BE248522.2|BE248522 NF006F07DT1F1060 Drought Medicago truncatula cDNA clone NF006F07DT
5', mRNA sequence
Length = 287
Score = 31.3 bits (62), Expect = 5.1
Identities = 12/26 (46%), Positives = 18/26 (69%)
Frame = +1 / +2
Query: 49 HSNPIFHIFPLIHMQSSWLWFSSTLQ 126
HSN + PL+H+ SS+L S+TL+
Sbjct: 80 HSNHFLSLLPLLHLHSSFLSGSTTLR 157
>gb|BQ137613.1|BQ137613 NF029A08NR1F1000 Nodulated root Medicago truncatula cDNA clone
NF029A08NR 5', mRNA sequence
Length = 1123
Score = 27.2 bits (53), Expect(2) = 5.5
Identities = 6/12 (50%), Positives = 10/12 (83%)
Frame = +2 / -3
Query: 2 SLRANCCSWWII 37
SL +CC+WW++
Sbjct: 932 SLWCSCCAWWVV 897
Score = 22.1 bits (42), Expect(2) = 5.5
Identities = 6/11 (54%), Positives = 9/11 (81%)
Frame = +1 / -3
Query: 70 IFPLIHMQSSW 102
+FPL+ + SSW
Sbjct: 92 LFPLVRVNSSW 60
>gb|AC152177.23| Medicago truncatula clone mth2-65h3, WORKING DRAFT SEQUENCE, 17
unordered pieces
Length = 257180
Score = 28.6 bits (56), Expect(2) = 6.6
Identities = 15/50 (30%), Positives = 26/50 (52%)
Frame = -3 / -3
Query: 214 FFSSASF*MVLTVTEIILESILPLFGVISTEEYLRTITTSFAYESMEKYE 65
FF++ S +VL T + LPL +S ++T + +FAY E+ +
Sbjct: 13860 FFNTFSTVLVLDSTYKTNKYHLPLLEFVSNTSTMKTFSIAFAYMMSERQD 13711
Score = 27.6 bits (54), Expect(2) = 6.6
Identities = 9/14 (64%), Positives = 12/14 (85%)
Frame = -3 / -2
Query: 253 IXNRKYGRGLNEAF 212
+ NR+YGRGLN+ F
Sbjct: 64483 VHNRRYGRGLNKNF 64442
>gb|AJ389047.1|AJ389047 AJ389047 Medicago truncatula R108 Medicago truncatula cDNA clone
MtNo424, mRNA sequence
Length = 508
Score = 30.9 bits (61), Expect = 7.0
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = +1 / -2
Query: 34 HLSNHHSNPIFHIFPLIHMQSSW 102
H +NH S+ FH+FP H Q+ +
Sbjct: 270 HYTNHQSHYQFHVFPQNHFQNPY 202
>gb|BG647956.1|BG647956 EST509575 HOGA Medicago truncatula cDNA clone pHOGA-18K10 5' end,
mRNA sequence
Length = 776
Score = 30.9 bits (61), Expect = 7.0
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = +1 / -2
Query: 34 HLSNHHSNPIFHIFPLIHMQSSW 102
H +NH S+ FH+FP H Q+ +
Sbjct: 445 HYTNHQSHYQFHVFPQNHFQNPY 377
>gb|DW017929.1|DW017929 EST1226890 MTY Medicago truncatula cDNA clone MTYAZ95, mRNA
sequence
Length = 761
Score = 30.9 bits (61), Expect = 7.0
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = +1 / -1
Query: 34 HLSNHHSNPIFHIFPLIHMQSSW 102
H +NH S+ FH+FP H Q+ +
Sbjct: 509 HYTNHQSHYQFHVFPQNHFQNPY 441
>gb|AC150779.1| Medicago truncatula chromosome 2 clone mth2-31a11, *** SEQUENCING IN
PROGRESS ***, 9 unordered pieces
Length = 113308
Score = 30.9 bits (61), Expect = 7.0
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +1 / +1
Query: 19 LLLVDHLSNHHSNPIFHIFPLIHMQ 93
LLL + SNH+ HIFPL H++
Sbjct: 52471 LLLTKNTSNHYKKT*RHIFPLTHIK 52545
>emb|CT009553.4| M.truncatula DNA sequence from clone MTH2-115C19 on chromosome 3,
complete sequence
Length = 125987
Score = 30.9 bits (61), Expect = 7.0
Identities = 14/30 (46%), Positives = 17/30 (56%)
Frame = +1 / +1
Query: 10 SKLLLLVDHLSNHHSNPIFHIFPLIHMQSS 99
+K L V HLS H I H+FP H+Q S
Sbjct: 110680 NKELSSVTHLSFQHVEGIHHLFPPHHLQHS 110769
>gb|BE325816.1|BE325816 NF020C02ST1F1007 Developing stem Medicago truncatula cDNA clone
NF020C02ST 5', mRNA sequence
Length = 673
Score = 30.4 bits (60), Expect = 9.6
Identities = 14/27 (51%), Positives = 14/27 (51%)
Frame = +1 / -2
Query: 7 PSKLLLLVDHLSNHHSNPIFHIFPLIH 87
P LL LVDHL NP FHI H
Sbjct: 396 PYSLLFLVDHLC*RIQNPDFHIETASH 316
>gb|BF636183.1|BF636183 NF106E12DT1F1097 Drought Medicago truncatula cDNA clone NF106E12DT
5', mRNA sequence
Length = 652
Score = 30.4 bits (60), Expect = 9.6
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = +1 / -1
Query: 49 HSNPIFHIFPLIHMQSSWLWF 111
HSNP+ PL+H QS+W F
Sbjct: 619 HSNPL*VSLPLVHPQSTWQIF 557
>gb|CX536396.1|CX536396 s13dNF22D05GS045_455966 Germinating Seed Medicago truncatula cDNA,
mRNA sequence
Length = 561
Score = 30.4 bits (60), Expect = 9.6
Identities = 11/30 (36%), Positives = 18/30 (60%)
Frame = +2 / +1
Query: 89 CKARGYGSQVLFSGDNTK*RQYTFQDYFCY 178
CK+ +GS+ F D +K +F+DY C+
Sbjct: 52 CKSENFGSKCDFESDFSKNSWSSFKDYNCF 141
>gb|AC121237.19| Medicago truncatula clone mth2-22g11, complete sequence
Length = 127731
Score = 30.4 bits (60), Expect = 9.6
Identities = 14/27 (51%), Positives = 14/27 (51%)
Frame = +1 / -3
Query: 7 PSKLLLLVDHLSNHHSNPIFHIFPLIH 87
P LL LVDHL NP FHI H
Sbjct: 85591 PYSLLFLVDHLC*RIQNPDFHIETASH 85511
>gb|AC130804.44| Medicago truncatula clone mth2-15l5, WORKING DRAFT SEQUENCE, 5 ordered
pieces
Length = 109715
Score = 30.4 bits (60), Expect = 9.6
Identities = 14/46 (30%), Positives = 22/46 (47%)
Frame = -2 / -3
Query: 278 SSKVIEPIYXQSEVWSGIK*SFLFLSIFLNGPHSNRNNLGKYTAFI 141
S ++ + EVW IK F F +FLNG + N + +F+
Sbjct: 69519 SDCILFKVATLDEVWQFIKCLFFFFLLFLNGQY*YGNCYANFFSFL 69382
>gb|AC174345.1| Medicago truncatula clone mth2-167k9, WORKING DRAFT SEQUENCE, 5
unordered pieces
Length = 109715
Score = 30.4 bits (60), Expect = 9.6
Identities = 14/46 (30%), Positives = 22/46 (47%)
Frame = -2 / -3
Query: 278 SSKVIEPIYXQSEVWSGIK*SFLFLSIFLNGPHSNRNNLGKYTAFI 141
S ++ + EVW IK F F +FLNG + N + +F+
Sbjct: 10308 SDCILFKVATLDEVWQFIKCLFFFFLLFLNGQY*YGNCYANFFSFL 10171
>gb|AC174345.5| Medicago truncatula clone mth2-167k9, WORKING DRAFT SEQUENCE, 15
unordered pieces
Length = 133406
Score = 30.4 bits (60), Expect = 9.6
Identities = 14/46 (30%), Positives = 22/46 (47%)
Frame = -2 / -2
Query: 278 SSKVIEPIYXQSEVWSGIK*SFLFLSIFLNGPHSNRNNLGKYTAFI 141
S ++ + EVW IK F F +FLNG + N + +F+
Sbjct: 72037 SDCILFKVATLDEVWQFIKCLFFFFLLFLNGQY*YGNCYANFFSFL 71900
>gb|AC174294.6| Medicago truncatula clone mth2-71d8, WORKING DRAFT SEQUENCE, 10
unordered pieces
Length = 151569
Score = 30.4 bits (60), Expect = 9.6
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = +1 / -1
Query: 4 SPSKLLLLVDHLSNHHSNPIFHIF 75
SP+KLLLL H++ FHIF
Sbjct: 9144 SPTKLLLLTTHMNQVEQRSHFHIF 9073
Database: Mt_nucl_with_EST.fasta
Posted date: May 2, 2006 2:46 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 295,791,034
Number of Sequences: 392609
Number of extensions: 3602182
Number of successful extensions: 127603
Number of sequences better than 10.0: 37
length of database: 147,244,331
effective HSP length: 51
effective length of database: 127,221,272
effective search space used: 6742727416
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)