TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAE37h07.yg.2.1
(642 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AC152887.20| Medicago truncatula clone mth2-91j4, WORKIN... 182 5e-048
gb|AC157648.18| Medicago truncatula clone mth2-68d9, comple... 182 5e-048
gb|AC158209.13| Medicago truncatula clone mth2-154h5, compl... 185 2e-047
gb|AC150782.12| Medicago truncatula clone mth2-116a21, WORK... 184 1e-045
gb|AC158736.12| Medicago truncatula clone mth2-165n2, WORKI... 184 1e-045
gb|AC152184.1| Medicago truncatula chromosome 7 BAC clone m... 178 8e-044
gb|AC122723.6| Medicago truncatula clone mth2-5i15, complet... 178 8e-044
gb|AC137603.16| Medicago truncatula clone mth2-14b10, compl... 100 6e-039
gb|AC170583.6| Medicago truncatula clone mth2-19b5, complet... 87 6e-037
gb|BG449853.1|BG449853 NF053H03IN1F1029 Insect herbivory Me... 142 3e-034
gb|BF641357.1|BF641357 NF061B11IN1F1092 Insect herbivory Me... 116 2e-025
gb|BQ147161.1|BQ147161 NF034H03FL1F1031 Developing flower M... 87 5e-021
>gb|AC152887.20| Medicago truncatula clone mth2-91j4, WORKING DRAFT SEQUENCE, 2 ordered
pieces
Length = 116277
Score = 182 bits (393), Expect(2) = 5e-048
Identities = 76/162 (46%), Positives = 95/162 (58%)
Frame = -2 / -1
Query: 635 AXNAQFLVXXGIXXXVXMIMGXILXLXXMXXVXXFITMQLQXCSVFXXFSLXTXXXXXGX 456
A +Q +V G+ + M+M L + FI MQLQ SVF F L T G
Sbjct: 97380 ALASQSVVQLGLLLVLPMVMEIGLEKGFRTALGDFIIMQLQLASVFFTFQLGTKAHYYGR 97201
Query: 455 XILHXXAKYRATXRGFVVXHIXFAENYRLYSXSHFVKALEVAXXXXXXIAYXXXXGGSSS 276
+LH +KYR T RGFVV H FA+NYR+YS SHFVK LE+ Y S+
Sbjct: 97200 TLLHGGSKYRPTGRGFVVFHAKFADNYRMYSRSHFVKGLEILILLIIYEVYGESYRSSTL 97021
Query: 275 FILITISSWFLVMSWLFAPYIFNPSGFEWQKTVEDFDDWTXW 150
+ ITIS WFL +SWLFAP++FNPSGF+WQKTV+D+ DW W
Sbjct: 97020 YFFITISMWFLAISWLFAPFLFNPSGFDWQKTVDDWSDWKRW 96895
Score = 29.5 bits (58), Expect(2) = 5e-048
Identities = 10/19 (52%), Positives = 14/19 (73%)
Frame = -2 / -1
Query: 62 RGRILETIXXXRFLMFQYG 6
RG+ILE + RF ++QYG
Sbjct: 96801 RGKILEIVLACRFFIYQYG 96745
>gb|AC157648.18| Medicago truncatula clone mth2-68d9, complete sequence
Length = 84361
Score = 182 bits (393), Expect(2) = 5e-048
Identities = 76/162 (46%), Positives = 95/162 (58%)
Frame = -2 / +2
Query: 635 AXNAQFLVXXGIXXXVXMIMGXILXLXXMXXVXXFITMQLQXCSVFXXFSLXTXXXXXGX 456
A +Q +V G+ + M+M L + FI MQLQ SVF F L T G
Sbjct: 76454 ALASQSVVQLGLLLVLPMVMEIGLEKGFRTALGDFIIMQLQLASVFFTFQLGTKAHYYGR 76633
Query: 455 XILHXXAKYRATXRGFVVXHIXFAENYRLYSXSHFVKALEVAXXXXXXIAYXXXXGGSSS 276
+LH +KYR T RGFVV H FA+NYR+YS SHFVK LE+ Y S+
Sbjct: 76634 TLLHGGSKYRPTGRGFVVFHAKFADNYRMYSRSHFVKGLEILILLIIYEVYGESYRSSTL 76813
Query: 275 FILITISSWFLVMSWLFAPYIFNPSGFEWQKTVEDFDDWTXW 150
+ ITIS WFL +SWLFAP++FNPSGF+WQKTV+D+ DW W
Sbjct: 76814 YFFITISMWFLAISWLFAPFLFNPSGFDWQKTVDDWSDWKRW 76939
Score = 29.5 bits (58), Expect(2) = 5e-048
Identities = 10/19 (52%), Positives = 14/19 (73%)
Frame = -2 / +2
Query: 62 RGRILETIXXXRFLMFQYG 6
RG+ILE + RF ++QYG
Sbjct: 77033 RGKILEIVLACRFFIYQYG 77089
>gb|AC158209.13| Medicago truncatula clone mth2-154h5, complete sequence
Length = 93864
Score = 185 bits (399), Expect(2) = 2e-047
Identities = 79/163 (48%), Positives = 99/163 (60%)
Frame = -2 / -2
Query: 638 AAXNAQFLVXXGIXXXVXMIMGXILXLXXMXXVXXFITMQLQXCSVFXXFSLXTXXXXXG 459
AA +Q LV G+ + MIM L + I MQLQ VF FSL T G
Sbjct: 56057 AAMASQSLVQIGLLMTLPMIMEIGLERGFRTALGDLIIMQLQLAPVFFTFSLGTKLHYFG 55878
Query: 458 XXILHXXAKYRATXRGFVVXHIXFAENYRLYSXSHFVKALEVAXXXXXXIAYXXXXGGSS 279
+LH AKYRAT RGFVV H FA+NYR+YS SHFVK +E+A + Y S+
Sbjct: 55877 RTLLHGGAKYRATGRGFVVRHEKFADNYRMYSRSHFVKGIELALLLICYMIYGAATPDST 55698
Query: 278 SFILITISSWFLVMSWLFAPYIFNPSGFEWQKTVEDFDDWTXW 150
++ L++ S WFLV SWLF+P++FNPSGFEWQK ED+DDW+ W
Sbjct: 55697 AYALLSCSMWFLVGSWLFSPFLFNPSGFEWQKIYEDWDDWSKW 55569
Score = 65.7 bits (137), Expect(3) = 3e-021
Identities = 42/107 (39%), Positives = 53/107 (49%)
Frame = +1 / +1
Query: 319 IRRRATSSALTK*LXE*SL*FSANXMCXTTKPLXVARYFAXX*RIXLPX*XXXVXSEKXK 498
+R +A S LTK*L E L* SAN C TTKP VA YFA R P * V SEK K
Sbjct: 55738 MRSKANSIPLTK*LLEYIL*LSANFSCRTTKPRPVALYFAPPCRRVRPK*CSFVPSEKVK 55917
Query: 499 NTEQXWSCIVIKXXTXXIXXSXRMXPIIIXTXXKIPXXTKN*AFXAA 639
T SCI+++ + +I+ + P T++* AA
Sbjct: 55918 KTGASCSCIIMRSPKAVLNPLSSPISMIMGRVIRRPI*TRD*EAIAA 56058
Score = 45.1 bits (92), Expect(3) = 3e-021
Identities = 17/32 (53%), Positives = 24/32 (75%)
Frame = +1 / +1
Query: 160 QSSKSSTVFCHSKPEGLKM*GAKSQDITRNQL 255
QSS+SS +FCHS P+GLK G +Q+ T+N +
Sbjct: 55579 QSSQSS*IFCHSNPDGLKRKGLNNQEPTKNHI 55674
Score = 29.5 bits (58), Expect(3) = 3e-021
Identities = 10/12 (83%), Positives = 11/12 (91%)
Frame = +1 / +1
Query: 76 CACSSSHQDSQL 111
C+CSSSH DSQL
Sbjct: 55495 CSCSSSHHDSQL 55530
Score = 24.9 bits (48), Expect(2) = 2e-047
Identities = 8/15 (53%), Positives = 9/15 (60%)
Frame = -1 / -1
Query: 138 RWSWCKGXXXLGILV 94
RW WC LGI+V
Sbjct: 55557 RWYWCSFNKELGIMV 55513
>gb|AC150782.12| Medicago truncatula clone mth2-116a21, WORKING DRAFT SEQUENCE, 3
unordered pieces
Length = 118726
Score = 184 bits (396), Expect = 1e-045
Identities = 78/166 (46%), Positives = 100/166 (60%)
Frame = -2 / +3
Query: 638 AAXNAQFLVXXGIXXXVXMIMGXILXLXXMXXVXXFITMQLQXCSVFXXFSLXTXXXXXG 459
A N QF++ G+ + MI+ L + + F+TMQLQ SVF FS+ T G
Sbjct: 21129 AILNQQFIIQLGLFTALPMIVENSLEHGFLQAIWDFLTMQLQLSSVFYTFSMGTRSHFFG 21308
Query: 458 XXILHXXAKYRATXRGFVVXHIXFAENYRLYSXSHFVKALEVAXXXXXXIAYXXXXGGSS 279
ILH AKYRAT RGFVV H FAE YRL+S SHFVKA+E+ + +
Sbjct: 21309 RTILHGGAKYRATGRGFVVEHKSFAEIYRLFSRSHFVKAIELGLILVIYATHSPVATDTF 21488
Query: 278 SFILITISSWFLVMSWLFAPYIFNPSGFEWQKTVEDFDDWTXWXXY 141
+I +TI+SWFLV SW+ AP++FNPSGF+W KTV DFDD+ W Y
Sbjct: 21489 VYIALTITSWFLVASWVVAPFVFNPSGFDWLKTVYDFDDFMNWIWY 21626
>gb|AC158736.12| Medicago truncatula clone mth2-165n2, WORKING DRAFT SEQUENCE
Length = 118519
Score = 184 bits (396), Expect = 1e-045
Identities = 78/166 (46%), Positives = 100/166 (60%)
Frame = -2 / -1
Query: 638 AAXNAQFLVXXGIXXXVXMIMGXILXLXXMXXVXXFITMQLQXCSVFXXFSLXTXXXXXG 459
A N QF++ G+ + MI+ L + + F+TMQLQ SVF FS+ T G
Sbjct: 11668 AILNQQFIIQLGLFTALPMIVENSLEHGFLQAIWDFLTMQLQLSSVFYTFSMGTRSHFFG 11489
Query: 458 XXILHXXAKYRATXRGFVVXHIXFAENYRLYSXSHFVKALEVAXXXXXXIAYXXXXGGSS 279
ILH AKYRAT RGFVV H FAE YRL+S SHFVKA+E+ + +
Sbjct: 11488 RTILHGGAKYRATGRGFVVEHKSFAEIYRLFSRSHFVKAIELGLILVIYATHSPVATDTF 11309
Query: 278 SFILITISSWFLVMSWLFAPYIFNPSGFEWQKTVEDFDDWTXWXXY 141
+I +TI+SWFLV SW+ AP++FNPSGF+W KTV DFDD+ W Y
Sbjct: 11308 VYIALTITSWFLVASWVVAPFVFNPSGFDWLKTVYDFDDFMNWIWY 11171
>gb|AC152184.1| Medicago truncatula chromosome 7 BAC clone mte1-61c3, complete
sequence
Length = 103090
Score = 178 bits (383), Expect = 8e-044
Identities = 75/162 (46%), Positives = 94/162 (58%)
Frame = -2 / +1
Query: 635 AXNAQFLVXXGIXXXVXMIMGXILXLXXMXXVXXFITMQLQXCSVFXXFSLXTXXXXXGX 456
A +Q V G + M+M L + FI MQLQ VF FSL T G
Sbjct: 8164 ALASQSFVQIGFLMALPMLMEIGLERGFRTALSEFILMQLQLAPVFFTFSLGTKTHYFGR 8343
Query: 455 XILHXXAKYRATXRGFVVXHIXFAENYRLYSXSHFVKALEVAXXXXXXIAYXXXXGGSSS 276
+LH AKYR T RGFVV H FA+NYRLYS SHFVK +E+ + + +
Sbjct: 8344 TLLHGGAKYRPTGRGFVVFHAKFADNYRLYSRSHFVKGIELLVLLVVYEIFSHSYRSAVA 8523
Query: 275 FILITISSWFLVMSWLFAPYIFNPSGFEWQKTVEDFDDWTXW 150
+ILIT+S WF+V +WLFAP++FNPSGFEWQK V+D+ DW W
Sbjct: 8524 YILITVSMWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWNKW 8649
>gb|AC122723.6| Medicago truncatula clone mth2-5i15, complete sequence
Length = 105353
Score = 178 bits (383), Expect = 8e-044
Identities = 75/162 (46%), Positives = 94/162 (58%)
Frame = -2 / +1
Query: 635 AXNAQFLVXXGIXXXVXMIMGXILXLXXMXXVXXFITMQLQXCSVFXXFSLXTXXXXXGX 456
A +Q V G + M+M L + FI MQLQ VF FSL T G
Sbjct: 79561 ALASQSFVQIGFLMALPMLMEIGLERGFRTALSEFILMQLQLAPVFFTFSLGTKTHYFGR 79740
Query: 455 XILHXXAKYRATXRGFVVXHIXFAENYRLYSXSHFVKALEVAXXXXXXIAYXXXXGGSSS 276
+LH AKYR T RGFVV H FA+NYRLYS SHFVK +E+ + + +
Sbjct: 79741 TLLHGGAKYRPTGRGFVVFHAKFADNYRLYSRSHFVKGIELLVLLVVYEIFSHSYRSAVA 79920
Query: 275 FILITISSWFLVMSWLFAPYIFNPSGFEWQKTVEDFDDWTXW 150
+ILIT+S WF+V +WLFAP++FNPSGFEWQK V+D+ DW W
Sbjct: 79921 YILITVSMWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWNKW 80046
>gb|AC137603.16| Medicago truncatula clone mth2-14b10, complete sequence
Length = 109793
Score = 100 bits (212), Expect(2) = 6e-039
Identities = 47/101 (46%), Positives = 55/101 (54%)
Frame = -2 / -1
Query: 635 AXNAQFLVXXGIXXXVXMIMGXILXLXXMXXVXXFITMQLQXCSVFXXFSLXTXXXXXGX 456
A +Q V G + M+M L + FI MQLQ VF FSL T G
Sbjct: 61898 ALASQSFVQIGFLMALPMLMEIGLERGFRTALSEFILMQLQLAPVFFTFSLGTKTHYYGR 61719
Query: 455 XILHXXAKYRATXRGFVVXHIXFAENYRLYSXSHFVKALEV 333
+LH AKYR T RGFVV H FA+NYRLYS SHFVK +E+
Sbjct: 61718 TLLHGGAKYRPTGRGFVVFHAKFADNYRLYSRSHFVKGIEL 61596
Score = 87.2 bits (184), Expect(3) = 5e-031
Identities = 32/53 (60%), Positives = 40/53 (75%)
Frame = -2 / -3
Query: 341 LEVAXXXXXXIAYXXXXGGSSSFILITISSWFLVMSWLFAPYIFNPSGFEWQK 183
LEV +AY GG+ S+IL++ISSWF+ +SWLFAPY+FNPSGFEWQK
Sbjct: 576 LEVVLLLIVYLAYGYNDGGALSYILLSISSWFMALSWLFAPYLFNPSGFEWQK 418
Score = 82.2 bits (173), Expect(2) = 6e-039
Identities = 26/43 (60%), Positives = 35/43 (81%)
Frame = -2 / -1
Query: 278 SFILITISSWFLVMSWLFAPYIFNPSGFEWQKTVEDFDDWTXW 150
S++LIT WF+V +WL+AP++FNPSGFEWQK V+D+ DW W
Sbjct: 61541 SYLLITTPMWFMVGTWLYAPFLFNPSGFEWQKIVDDWTDWNKW 61413
Score = 59.3 bits (123), Expect(3) = 5e-031
Identities = 23/30 (76%), Positives = 26/30 (86%)
Frame = -2 / -2
Query: 434 KYRATXRGFVVXHIXFAENYRLYSXSHFVK 345
+Y+AT RGFVV HI F+ENYRLYS SHFVK
Sbjct: 1924 QYQATGRGFVVRHIKFSENYRLYSRSHFVK 1835
Score = 44.1 bits (90), Expect(3) = 5e-031
Identities = 19/40 (47%), Positives = 22/40 (55%)
Frame = -2 / -2
Query: 551 MXXVXXFITMQLQXCSVFXXFSLXTXXXXXGXXILHXXAK 432
+ V FITMQ Q C+VF FSL T G ILH A+
Sbjct: 2113 LQAVVNFITMQFQLCTVFFTFSLGTRTHYFGRTILHGGAR 1994
>gb|AC170583.6| Medicago truncatula clone mth2-19b5, complete sequence
Length = 113632
Score = 87.2 bits (184), Expect(5) = 6e-037
Identities = 32/53 (60%), Positives = 40/53 (75%)
Frame = -2 / +2
Query: 341 LEVAXXXXXXIAYXXXXGGSSSFILITISSWFLVMSWLFAPYIFNPSGFEWQK 183
LEV +AY GG+ S+IL++ISSWF+ +SWLFAPY+FNPSGFEWQK
Sbjct: 9704 LEVVLLLIVYLAYGYNDGGALSYILLSISSWFMALSWLFAPYLFNPSGFEWQK 9862
Score = 59.3 bits (123), Expect(5) = 6e-037
Identities = 23/30 (76%), Positives = 26/30 (86%)
Frame = -2 / +1
Query: 434 KYRATXRGFVVXHIXFAENYRLYSXSHFVK 345
+Y+AT RGFVV HI F+ENYRLYS SHFVK
Sbjct: 8356 QYQATGRGFVVRHIKFSENYRLYSRSHFVK 8445
Score = 44.1 bits (90), Expect(5) = 6e-037
Identities = 19/40 (47%), Positives = 22/40 (55%)
Frame = -2 / +1
Query: 551 MXXVXXFITMQLQXCSVFXXFSLXTXXXXXGXXILHXXAK 432
+ V FITMQ Q C+VF FSL T G ILH A+
Sbjct: 8167 LQAVVNFITMQFQLCTVFFTFSLGTRTHYFGRTILHGGAR 8286
Score = 38.2 bits (77), Expect(5) = 6e-037
Identities = 15/26 (57%), Positives = 18/26 (69%)
Frame = -2 / +2
Query: 83 QAHIQTFRGRILETIXXXRFLMFQYG 6
QAHI++ RI ETI RF +FQYG
Sbjct: 10532 QAHIRSLGSRIAETILSLRFFIFQYG 10609
Score = 30.4 bits (60), Expect(5) = 6e-037
Identities = 12/23 (52%), Positives = 13/23 (56%)
Frame = -1 / +3
Query: 162 LDXXVXL*RWSWCKGXXXLGILV 94
LD L RW+WC G LG LV
Sbjct: 10221 LDKLATLQRWNWC*GRRKLGSLV 10289
>gb|BG449853.1|BG449853 NF053H03IN1F1029 Insect herbivory Medicago truncatula cDNA clone
NF053H03IN 5', mRNA sequence
Length = 646
Score = 142 bits (304), Expect(2) = 3e-034
Identities = 53/101 (52%), Positives = 69/101 (68%)
Frame = -2 / +3
Query: 452 ILHXXAKYRATXRGFVVXHIXFAENYRLYSXSHFVKALEVAXXXXXXIAYXXXXGGSSSF 273
+LH A+YR T RGFVV H FA+NYRLYS SHFVK +E+ + G ++
Sbjct: 9 LLHGGAQYRGTGRGFVVFHAKFADNYRLYSRSHFVKGIELVILLVVYHIFGHAYRGVVAY 188
Query: 272 ILITISSWFLVMSWLFAPYIFNPSGFEWQKTVEDFDDWTXW 150
ILITI+ WF+ +WLFAP++FNPSGFEWQK ++D+ DW W
Sbjct: 189 ILITITIWFMAGTWLFAPFLFNPSGFEWQKILDDWTDWHKW 311
Score = 24.4 bits (47), Expect(2) = 3e-034
Identities = 8/16 (50%), Positives = 10/16 (62%)
Frame = -1 / +2
Query: 48 *DNXQX*ISHVSVWXC 1
*DN I H+S+W C
Sbjct: 419 *DNTGTPIFHISIWTC 466
>gb|BF641357.1|BF641357 NF061B11IN1F1092 Insect herbivory Medicago truncatula cDNA clone
NF061B11IN 5', mRNA sequence
Length = 660
Score = 116 bits (249), Expect = 2e-025
Identities = 55/128 (42%), Positives = 72/128 (56%)
Frame = -2 / +1
Query: 638 AAXNAQFLVXXGIXXXVXMIMGXILXLXXMXXVXXFITMQLQXCSVFXXFSLXTXXXXXG 459
A N QF++ GI + M++ L + V F+TMQLQ S+F FSL T G
Sbjct: 277 AIVNQQFIIQLGIFTALPMVVENTLEHGFLPAVWDFLTMQLQLGSLFFTFSLGTRTHFFG 456
Query: 458 XXILHXXAKYRATXRGFVVXHIXFAENYRLYSXSHFVKALEVAXXXXXXIAYXXXXGGSS 279
ILH AKYRAT RGFVV H FAENYRLY+ SHFVKA+E+ ++ +
Sbjct: 457 RTILHGGAKYRATGRGFVVEHKSFAENYRLYARSHFVKAIELGIILIVYASHSPLPKATF 636
Query: 278 SFILITIS 255
+I +T+S
Sbjct: 637 VYIAMTLS 660
>gb|BQ147161.1|BQ147161 NF034H03FL1F1031 Developing flower Medicago truncatula cDNA clone
NF034H03FL 5', mRNA sequence
Length = 266
Score = 87.2 bits (184), Expect(2) = 5e-021
Identities = 28/43 (65%), Positives = 37/43 (86%)
Frame = -2 / +2
Query: 278 SFILITISSWFLVMSWLFAPYIFNPSGFEWQKTVEDFDDWTXW 150
++ILIT+S WF+V +WLFAP++FNPSGFEWQK V+D+ DW W
Sbjct: 137 AYILITVSMWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWNKW 265
Score = 34.1 bits (68), Expect(2) = 5e-021
Identities = 12/16 (75%), Positives = 14/16 (87%)
Frame = -2 / +3
Query: 383 ENYRLYSXSHFVKALE 336
+NYRLYS SHFVK +E
Sbjct: 30 DNYRLYSRSHFVKGIE 77
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 369,286,199
Number of Sequences: 392609
Number of extensions: 3156191
Number of successful extensions: 15686
Number of sequences better than 1.0e-020: 17
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 20040907755
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)