TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AF332176.2.1
(912 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG453428.1|BG453428 NF091G11LF1F1085 Developing leaf Med... 112 4e-040
gb|BG455193.1|BG455193 NF100G04PL1F1034 Phosphate starved l... 91 2e-035
gb|AC148226.7| Medicago truncatula clone mth2-5i20, complet... 51 2e-032
gb|AC174309.3| Medicago truncatula clone mth2-139k12, WORKI... 51 9e-032
gb|CA917715.1|CA917715 EST641862 GPOD Medicago truncatula c... 91 8e-030
gb|AL379968.1|AL379968 MtBB48E05F1 MtBB Medicago truncatula... 91 4e-027
gb|BG448066.1|BG448066 NF105C08EC1F1056 Elicited cell cultu... 79 7e-024
>gb|BG453428.1|BG453428 NF091G11LF1F1085 Developing leaf Medicago truncatula cDNA clone
NF091G11LF 5', mRNA sequence
Length = 638
Score = 112 bits (240), Expect(3) = 4e-040
Identities = 44/80 (55%), Positives = 59/80 (73%)
Frame = +2 / +3
Query: 203 APYHFDLSGTAFGAMAKDEHNDELRHAGIIDIQFKRVPCQYPGLTVTFHIERGSNPNYLA 382
A YHFDLSG+AFG+MA ++ELR+AG I I+ +RV C Y G T+ FH++ GSN Y A
Sbjct: 18 AEYHFDLSGSAFGSMAVSGEDEELRNAGKIVIEHRRVQCNYGGSTIAFHVDSGSNQEYFA 197
Query: 383 VLVEYENGDGDVVQVDLMES 442
L+EYE GDGD+ +V+L E+
Sbjct: 198 ALIEYEEGDGDLNKVELKEA 257
Score = 46.9 bits (96), Expect(3) = 4e-040
Identities = 13/29 (44%), Positives = 22/29 (75%)
Frame = +2 / +3
Query: 473 WEPMRESWGSIWRMDTRRPLQGPFSLRVT 559
W+ M++SWG++W+ D PL+ P S+R+T
Sbjct: 273 WDTMQQSWGAVWKFDKGAPLKAPISIRLT 359
Score = 45.1 bits (92), Expect(3) = 4e-040
Identities = 18/30 (60%), Positives = 21/30 (70%)
Frame = +2 / +3
Query: 557 TNESGKTLVADQVIPADWQPDNVYSSIVQF 646
T +SGKT+VA VIPA W+P Y SIV F
Sbjct: 360 TLKSGKTIVAHNVIPAGWKPGQTYRSIVNF 449
>gb|BG455193.1|BG455193 NF100G04PL1F1034 Phosphate starved leaf Medicago truncatula cDNA
clone NF100G04PL 5', mRNA sequence
Length = 654
Score = 90.9 bits (192), Expect(3) = 2e-035
Identities = 34/81 (41%), Positives = 51/81 (62%)
Frame = +2 / +2
Query: 212 HFDLSGTAFGAMAKDEHNDELRHAGIIDIQFKRVPCQYPGLTVTFHIERGSNPNYLAVLV 391
HFDLSG AFG MA N +LR+ G I + ++R C Y G + F + GS P +L++LV
Sbjct: 206 HFDLSGAAFGHMAVHGENGQLRNRGEIQVLYRRTTCVYAGKNIAFQVNEGSTPFWLSLLV 385
Query: 392 EYENGDGDVVQVDLMESSPDD 454
E+E+GDGD+ + + E+ +
Sbjct: 386 EFEDGDGDISSMHIQEAGSSE 448
Score = 56.1 bits (116), Expect(3) = 2e-035
Identities = 21/49 (42%), Positives = 29/49 (59%)
Frame = +2 / +2
Query: 38 VNMPPFSAMTSCGNEPIFKDGKGCGSCYQIRCKAHPACSGVAETVIITD 184
V++ P A ++K G+GCG CY+++C H CS A TVIITD
Sbjct: 29 VDVKPLRARVGAVGPVLYKKGEGCGECYKVKCLDHTICSKRAVTVIITD 175
Score = 42.3 bits (86), Expect(3) = 2e-035
Identities = 14/28 (50%), Positives = 22/28 (78%)
Frame = +2 / +2
Query: 527 PLQGPFSLRVTNESGKTLVADQVIPADW 610
PL+GPFS++++ +GK+L A VIP+ W
Sbjct: 500 PLRGPFSVKLSTSTGKSLTAKDVIPSHW 583
>gb|AC148226.7| Medicago truncatula clone mth2-5i20, complete sequence
Length = 84259
Score = 51.0 bits (105), Expect(6) = 2e-032
Identities = 19/39 (48%), Positives = 29/39 (74%)
Frame = +2 / +1
Query: 329 GLTVTFHIERGSNPNYLAVLVEYENGDGDVVQVDLMESS 445
G ++ F I+ G+NP A +EYENG+GD+VQV+L +S+
Sbjct: 65848 GNSIVFTIDNGANPYCFATEIEYENGNGDLVQVELSQSN 65964
Score = 49.6 bits (102), Expect(6) = 2e-032
Identities = 19/40 (47%), Positives = 23/40 (57%)
Frame = +2 / +2
Query: 8 VGGGACGFKGVNMPPFSAMTSCGNEPIFKDGKGCGSCYQI 127
VGG + V PP S M S G IF G+GCG+CYQ+
Sbjct: 63989 VGGACGYVESVENPPLSKMISAGGPSIFLGGRGCGACYQV 64108
Score = 48.7 bits (100), Expect(6) = 2e-032
Identities = 21/40 (52%), Positives = 27/40 (67%)
Frame = +2 / +1
Query: 212 HFDLSGTAFGAMAKDEHNDELRHAGIIDIQFKRVPCQYPG 331
HFDLSGTAFG++A D LR+AG I+I++ R PG
Sbjct: 65611 HFDLSGTAFGSLANPNQADNLRNAGKINIRYTRYLGFKPG 65730
Score = 45.1 bits (92), Expect(6) = 2e-032
Identities = 15/31 (48%), Positives = 20/31 (64%)
Frame = +2 / +1
Query: 464 TGVWEPMRESWGSIWRMDTRRPLQGPFSLRV 556
+ W PM SWGS W +T PLQ PFS+++
Sbjct: 65959 SNTWHPMFRSWGSRWAFNTGSPLQTPFSIKL 66051
Score = 42.8 bits (87), Expect(6) = 2e-032
Identities = 15/29 (51%), Positives = 20/29 (68%)
Frame = +2 / +3
Query: 560 NESGKTLVADQVIPADWQPDNVYSSIVQF 646
N S T++A+ VIP +WQP VY S+V F
Sbjct: 70653 NGSNNTIIAENVIPQNWQPGQVYRSLVNF 70739
Score = 30.4 bits (60), Expect(6) = 2e-032
Identities = 10/22 (45%), Positives = 15/22 (68%)
Frame = +2 / +1
Query: 119 YQIRCKAHPACSGVAETVIITD 184
+Q++C + ACSG V+ITD
Sbjct: 65521 FQVKCTENSACSGSPVNVVITD 65586
>gb|AC174309.3| Medicago truncatula clone mth2-139k12, WORKING DRAFT SEQUENCE, 2
unordered pieces
Length = 111023
Score = 51.0 bits (105), Expect(6) = 9e-032
Identities = 19/39 (48%), Positives = 29/39 (74%)
Frame = +2 / +2
Query: 329 GLTVTFHIERGSNPNYLAVLVEYENGDGDVVQVDLMESS 445
G ++ F I+ G+NP A +EYENG+GD+VQV+L +S+
Sbjct: 92621 GNSIVFTIDNGANPYCFATEIEYENGNGDLVQVELSQSN 92737
Score = 49.6 bits (102), Expect(6) = 9e-032
Identities = 19/40 (47%), Positives = 23/40 (57%)
Frame = +2 / +3
Query: 8 VGGGACGFKGVNMPPFSAMTSCGNEPIFKDGKGCGSCYQI 127
VGG + V PP S M S G IF G+GCG+CYQ+
Sbjct: 90762 VGGACGYVESVENPPLSKMISAGGPSIFLGGRGCGACYQV 90881
Score = 48.7 bits (100), Expect(6) = 9e-032
Identities = 21/40 (52%), Positives = 27/40 (67%)
Frame = +2 / +2
Query: 212 HFDLSGTAFGAMAKDEHNDELRHAGIIDIQFKRVPCQYPG 331
HFDLSGTAFG++A D LR+AG I+I++ R PG
Sbjct: 92384 HFDLSGTAFGSLANPNQADNLRNAGKINIRYTRYLGFKPG 92503
Score = 45.1 bits (92), Expect(6) = 9e-032
Identities = 15/31 (48%), Positives = 20/31 (64%)
Frame = +2 / +2
Query: 464 TGVWEPMRESWGSIWRMDTRRPLQGPFSLRV 556
+ W PM SWGS W +T PLQ PFS+++
Sbjct: 92732 SNTWHPMFRSWGSRWAFNTGSPLQTPFSIKL 92824
Score = 42.8 bits (87), Expect(6) = 9e-032
Identities = 15/29 (51%), Positives = 20/29 (68%)
Frame = +2 / +1
Query: 560 NESGKTLVADQVIPADWQPDNVYSSIVQF 646
N S T++A+ VIP +WQP VY S+V F
Sbjct: 97426 NGSNNTIIAENVIPQNWQPGQVYRSLVNF 97512
Score = 30.4 bits (60), Expect(6) = 9e-032
Identities = 10/22 (45%), Positives = 15/22 (68%)
Frame = +2 / +2
Query: 119 YQIRCKAHPACSGVAETVIITD 184
+Q++C + ACSG V+ITD
Sbjct: 92294 FQVKCTENSACSGSPVNVVITD 92359
>gb|CA917715.1|CA917715 EST641862 GPOD Medicago truncatula cDNA clone GPOD-37E9, mRNA
sequence
Length = 769
Score = 90.9 bits (192), Expect(3) = 8e-030
Identities = 34/81 (41%), Positives = 51/81 (62%)
Frame = +2 / +2
Query: 212 HFDLSGTAFGAMAKDEHNDELRHAGIIDIQFKRVPCQYPGLTVTFHIERGSNPNYLAVLV 391
HFDLSG AFG MA N +LR+ G I + ++R C Y G + F + GS P +L++LV
Sbjct: 458 HFDLSGAAFGHMAVHGENGQLRNRGEIQVLYRRTTCVYAGKNIAFQVNEGSTPFWLSLLV 637
Query: 392 EYENGDGDVVQVDLMESSPDD 454
E+E+GDGD+ + + E+ +
Sbjct: 638 EFEDGDGDISSMHIQEAGSSE 700
Score = 54.2 bits (112), Expect(3) = 8e-030
Identities = 23/61 (37%), Positives = 31/61 (50%)
Frame = +2 / +2
Query: 2 GRVGGGACGFKGVNMPPFSAMTSCGNEPIFKDGKGCGSCYQIRCKAHPACSGVAETVIIT 181
G GG V++ P A ++ G+GCG CY+++C H CS A TVIIT
Sbjct: 245 GSRGGACGYGSLVDVKPLRARVGAVGPVLYNKGEGCGECYKVKCLDHTICSKRAVTVIIT 424
Query: 182 D 184
D
Sbjct: 425 D 427
Score = 24.9 bits (48), Expect(3) = 8e-030
Identities = 7/14 (50%), Positives = 8/14 (57%)
Frame = +2 / +2
Query: 473 WEPMRESWGSIWRM 514
W M WG+IW M
Sbjct: 701 WLQMNHLWGAIWNM 742
>gb|AL379968.1|AL379968 MtBB48E05F1 MtBB Medicago truncatula cDNA clone MtBB48E05 T3, mRNA
sequence
Length = 476
Score = 90.9 bits (192), Expect(2) = 4e-027
Identities = 34/81 (41%), Positives = 51/81 (62%)
Frame = +2 / +2
Query: 212 HFDLSGTAFGAMAKDEHNDELRHAGIIDIQFKRVPCQYPGLTVTFHIERGSNPNYLAVLV 391
HFDLSG AFG MA N +LR+ G I + ++R C Y G + F + GS P +L++LV
Sbjct: 134 HFDLSGAAFGHMAVHGENGQLRNRGEIQVLYRRTTCVYAGKNIAFQVNEGSTPFWLSLLV 313
Query: 392 EYENGDGDVVQVDLMESSPDD 454
E+E+GDGD+ + + E+ +
Sbjct: 314 EFEDGDGDISSMHIQEAGSSE 376
Score = 52.4 bits (108), Expect(2) = 4e-027
Identities = 18/33 (54%), Positives = 24/33 (72%)
Frame = +2 / +2
Query: 86 IFKDGKGCGSCYQIRCKAHPACSGVAETVIITD 184
++K G+GCG CY+++C H CS A TVIITD
Sbjct: 5 LYKKGEGCGECYKVKCLDHTICSKRAVTVIITD 103
>gb|BG448066.1|BG448066 NF105C08EC1F1056 Elicited cell culture Medicago truncatula cDNA
clone NF105C08EC 5', mRNA sequence
Length = 652
Score = 79.0 bits (166), Expect(2) = 7e-024
Identities = 30/64 (46%), Positives = 41/64 (64%)
Frame = +2 / +1
Query: 197 PVAPYHFDLSGTAFGAMAKDEHNDELRHAGIIDIQFKRVPCQYPGLTVTFHIERGSNPNY 376
P HFDLSG AFG MA N +LR+ G I + ++R PC+YPG + FH+ GS P +
Sbjct: 460 PTDTTHFDLSGAAFGHMAISGENGQLRNRGQIPVIYRRTPCKYPGKKIAFHVNEGSTPFW 639
Query: 377 LAVL 388
L++L
Sbjct: 640 LSLL 651
Score = 53.3 bits (110), Expect(2) = 7e-024
Identities = 23/61 (37%), Positives = 32/61 (52%)
Frame = +2 / +1
Query: 2 GRVGGGACGFKGVNMPPFSAMTSCGNEPIFKDGKGCGSCYQIRCKAHPACSGVAETVIIT 181
G GG V++ P A +F G+GCG+CY+++C + CS A TVIIT
Sbjct: 262 GSTGGACGYGTLVDVKPLRARVGAVGPVLFMKGEGCGACYKVKCLDNSICSRRAVTVIIT 441
Query: 182 D 184
D
Sbjct: 442 D 444
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 660,217,578
Number of Sequences: 392609
Number of extensions: 8046755
Number of successful extensions: 41971
Number of sequences better than 1.0e-020: 11
length of database: 147,244,331
effective HSP length: 55
effective length of database: 125,650,836
effective search space used: 31161407328
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)