TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 8636621.2.1
(691 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CB891385.1|CB891385 EST648354 KV3 Medicago truncatula cD... 157 1e-037
gb|AL370720.1|AL370720 MtBA39E07R1 MtBA Medicago truncatula... 155 3e-037
gb|AL371719.1|AL371719 MtBA46A11R1 MtBA Medicago truncatula... 152 1e-036
gb|AL369226.1|AL369226 MtBA29F07R1 MtBA Medicago truncatula... 127 1e-028
gb|BQ123421.1|BQ123421 EST608997 GLSD Medicago truncatula c... 117 2e-025
gb|BG647070.1|BG647070 EST508689 HOGA Medicago truncatula c... 115 9e-025
gb|BG645039.1|BG645039 EST506658 KV3 Medicago truncatula cD... 111 2e-024
gb|AC160013.10| Medicago truncatula clone mth2-162b23, WORK... 91 7e-024
gb|AC159535.5| Medicago truncatula clone mth2-152f22, WORKI... 91 8e-024
>gb|CB891385.1|CB891385 EST648354 KV3 Medicago truncatula cDNA clone KV3-50E15, mRNA
sequence
Length = 724
Score = 157 bits (338), Expect = 1e-037
Identities = 66/111 (59%), Positives = 79/111 (71%)
Frame = -3 / -1
Query: 689 LLQFLAIAAFTEEDLSRWKVRSGGELFTILYAGLVASGVAFALQIWCIDRGGPLFTAVFQ 510
L+QFL IA E D W +SGGE+FTILYAG+VASG+AFA+QIWCIDRGGP+F AV+Q
Sbjct: 622 LIQFLIIALILERDAQAWIFQSGGEVFTILYAGVVASGIAFAVQIWCIDRGGPVFVAVYQ 443
Query: 509 PVQTVAVAVMASAILGDQLYTXXXXXXXXXXXXLYFVLWGKSAEKKGARNL 357
PVQT+ VA+MAS LG++ Y LY VLWGK+ EKK AR L
Sbjct: 442 PVQTLVVAIMASLALGEEFYLGGIIGAVLIIVGLYLVLWGKNEEKKFAREL 290
Score = 137 bits (293), Expect = 2e-031
Identities = 62/109 (56%), Positives = 74/109 (67%)
Frame = +1 / +3
Query: 364 LAPFFSALLPHSTK*RPMTISTAPMIPPV*SWSPRMAEAMTATATVCTGWKTAVKSGPPL 543
LA FFS+ LPH T+ P I+TAPMIPP *+ SP+ EA+ AT V TG TA K+GPPL
Sbjct: 297 LANFFSSFLPHKTRYNPTMINTAPMIPPK*NSSPKAREAIIATTRV*TG*YTATKTGPPL 476
Query: 544 SMHQICRANATPDATRPAYRMVKSSPPERTFHLLRSSSVKAAMARNWRS 690
SMH IC ANA PDAT PAY++VK+SPP+ S S A+ RNW S
Sbjct: 477 SMHHICTANAIPDATTPAYKIVKTSPPDWKIQA*ASLSRIKAIMRNWIS 623
>gb|AL370720.1|AL370720 MtBA39E07R1 MtBA Medicago truncatula cDNA clone MtBA39E07 T7, mRNA
sequence
Length = 509
Score = 155 bits (332), Expect(2) = 3e-037
Identities = 65/109 (59%), Positives = 77/109 (70%)
Frame = -3 / +2
Query: 683 QFLAIAAFTEEDLSRWKVRSGGELFTILYAGLVASGVAFALQIWCIDRGGPLFTAVFQPV 504
QFL IA E D W +SGGE+FTILYAG+VASG+AFA+QIWCIDRGGP+F AV+QPV
Sbjct: 2 QFLIIALILERDAQAWIFQSGGEVFTILYAGVVASGIAFAVQIWCIDRGGPVFVAVYQPV 181
Query: 503 QTVAVAVMASAILGDQLYTXXXXXXXXXXXXLYFVLWGKSAEKKGARNL 357
QT+ VA+MAS LG++ Y LY VLWGK+ EKK AR L
Sbjct: 182 QTLVVAIMASLALGEEFYLGGIIGAVLIIVGLYLVLWGKNEEKKFAREL 328
Score = 136 bits (292), Expect = 3e-031
Identities = 61/107 (57%), Positives = 73/107 (68%)
Frame = +1 / -3
Query: 364 LAPFFSALLPHSTK*RPMTISTAPMIPPV*SWSPRMAEAMTATATVCTGWKTAVKSGPPL 543
LA FFS+ LPH T+ P I+TAPMIPP *+ SP+ EA+ AT V TG TA K+GPPL
Sbjct: 321 LANFFSSFLPHKTRYNPTMINTAPMIPPK*NSSPKAREAIIATTRV*TG*YTATKTGPPL 142
Query: 544 SMHQICRANATPDATRPAYRMVKSSPPERTFHLLRSSSVKAAMARNW 684
SMH IC ANA PDAT PAY++VK+SPP+ S S A+ RNW
Sbjct: 141 SMHHICTANAIPDATTPAYKIVKTSPPDWKIQA*ASLSRIKAIMRNW 1
Score = 23.1 bits (44), Expect(2) = 3e-037
Identities = 7/13 (53%), Positives = 10/13 (76%)
Frame = -2 / +3
Query: 39 HFIHNSYQKKKKK 1
H++ Y+KKKKK
Sbjct: 471 HYLEKIYKKKKKK 509
>gb|AL371719.1|AL371719 MtBA46A11R1 MtBA Medicago truncatula cDNA clone MtBA46A11 T7, mRNA
sequence
Length = 505
Score = 152 bits (327), Expect(2) = 1e-036
Identities = 64/108 (59%), Positives = 76/108 (70%)
Frame = -3 / +1
Query: 680 FLAIAAFTEEDLSRWKVRSGGELFTILYAGLVASGVAFALQIWCIDRGGPLFTAVFQPVQ 501
FL IA E D W +SGGE+FTILYAG+VASG+AFA+QIWCIDRGGP+F AV+QPVQ
Sbjct: 1 FLIIALILERDAQAWIFQSGGEVFTILYAGVVASGIAFAVQIWCIDRGGPVFVAVYQPVQ 180
Query: 500 TVAVAVMASAILGDQLYTXXXXXXXXXXXXLYFVLWGKSAEKKGARNL 357
T+ VA+MAS LG++ Y LY VLWGK+ EKK AR L
Sbjct: 181 TLVVAIMASLALGEEFYLGGIIGAVLIIVGLYLVLWGKNEEKKFAREL 324
Score = 129 bits (277), Expect = 4e-029
Identities = 55/88 (62%), Positives = 66/88 (75%)
Frame = +1 / -3
Query: 364 LAPFFSALLPHSTK*RPMTISTAPMIPPV*SWSPRMAEAMTATATVCTGWKTAVKSGPPL 543
LA FFS+ LPH T+ P I+TAPMIPP *+ SP+ EA+ AT V TG TA K+GPPL
Sbjct: 317 LANFFSSFLPHKTRYNPTMINTAPMIPPK*NSSPKAREAIIATTRV*TG*YTATKTGPPL 138
Query: 544 SMHQICRANATPDATRPAYRMVKSSPPE 627
SMH IC ANA PDAT PAY++VK+SPP+
Sbjct: 137 SMHHICTANAIPDATTPAYKIVKTSPPD 54
Score = 23.1 bits (44), Expect(2) = 1e-036
Identities = 7/13 (53%), Positives = 10/13 (76%)
Frame = -2 / +2
Query: 39 HFIHNSYQKKKKK 1
H++ Y+KKKKK
Sbjct: 467 HYLEKIYKKKKKK 505
>gb|AL369226.1|AL369226 MtBA29F07R1 MtBA Medicago truncatula cDNA clone MtBA29F07 T7, mRNA
sequence
Length = 440
Score = 127 bits (273), Expect = 1e-028
Identities = 53/85 (62%), Positives = 63/85 (74%)
Frame = -3 / +3
Query: 611 FTILYAGLVASGVAFALQIWCIDRGGPLFTAVFQPVQTVAVAVMASAILGDQLYTXXXXX 432
FTILYAG+VASG+AFA+QIWCIDRGGP+F AV+QPVQT+ VA+MAS LG++ Y
Sbjct: 3 FTILYAGVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTLVVAIMASLALGEEFYLGGIIG 182
Query: 431 XXXXXXXLYFVLWGKSAEKKGARNL 357
LY VLWGK+ EKK AR L
Sbjct: 183 AVLIIVGLYLVLWGKNEEKKFAREL 257
Score = 120 bits (256), Expect = 3e-026
Identities = 52/83 (62%), Positives = 61/83 (73%)
Frame = +1 / -2
Query: 364 LAPFFSALLPHSTK*RPMTISTAPMIPPV*SWSPRMAEAMTATATVCTGWKTAVKSGPPL 543
LA FFS+ LPH T+ P I+TAPMIPP *+ SP+ EA+ AT V TG TA K+GPPL
Sbjct: 250 LANFFSSFLPHKTRYNPTMINTAPMIPPK*NSSPKAREAIIATTRV*TG*YTATKTGPPL 71
Query: 544 SMHQICRANATPDATRPAYRMVK 612
SMH IC ANA PDAT PAY++VK
Sbjct: 70 SMHHICTANAIPDATTPAYKIVK 2
>gb|BQ123421.1|BQ123421 EST608997 GLSD Medicago truncatula cDNA clone pGLSD-32M5, mRNA
sequence
Length = 421
Score = 117 bits (250), Expect = 2e-025
Identities = 46/67 (68%), Positives = 55/67 (82%)
Frame = -3 / +1
Query: 689 LLQFLAIAAFTEEDLSRWKVRSGGELFTILYAGLVASGVAFALQIWCIDRGGPLFTAVFQ 510
L+QFL IA E D W +SGGE+FTILYAG+VASG+AFA+QIWCIDRGGP+F AV+Q
Sbjct: 220 LIQFLIIALILERDAQAWIFQSGGEVFTILYAGVVASGIAFAVQIWCIDRGGPVFVAVYQ 399
Query: 509 PVQTVAV 489
PVQT+ V
Sbjct: 400 PVQTLVV 420
>gb|BG647070.1|BG647070 EST508689 HOGA Medicago truncatula cDNA clone pHOGA-15J3 5' end,
mRNA sequence
Length = 631
Score = 115 bits (245), Expect = 9e-025
Identities = 45/64 (70%), Positives = 53/64 (82%)
Frame = -3 / +2
Query: 689 LLQFLAIAAFTEEDLSRWKVRSGGELFTILYAGLVASGVAFALQIWCIDRGGPLFTAVFQ 510
L+QFL IA E D W +SGGE+FTILYAG+VASG+AFA+QIWCIDRGGP+F AV+Q
Sbjct: 440 LIQFLIIALILERDAQAWIFQSGGEVFTILYAGVVASGIAFAVQIWCIDRGGPVFVAVYQ 619
Query: 509 PVQT 498
PVQT
Sbjct: 620 PVQT 631
>gb|BG645039.1|BG645039 EST506658 KV3 Medicago truncatula cDNA clone pKV3-39E21 5' end,
mRNA sequence
Length = 777
Score = 111 bits (236), Expect(2) = 2e-024
Identities = 43/63 (68%), Positives = 52/63 (82%)
Frame = -3 / +2
Query: 689 LLQFLAIAAFTEEDLSRWKVRSGGELFTILYAGLVASGVAFALQIWCIDRGGPLFTAVFQ 510
L+QFL IA E D W +SGGE+FTILYAG+VASG+AFA+QIWCIDRGGP+F AV+Q
Sbjct: 515 LIQFLIIALILERDAQAWIFQSGGEVFTILYAGVVASGIAFAVQIWCIDRGGPVFVAVYQ 694
Query: 509 PVQ 501
PV+
Sbjct: 695 PVK 703
Score = 22.6 bits (43), Expect(2) = 2e-024
Identities = 7/8 (87%), Positives = 7/8 (87%)
Frame = -2 / +3
Query: 453 LHGRDHWS 430
L GRDHWS
Sbjct: 750 LFGRDHWS 773
>gb|AC160013.10| Medicago truncatula clone mth2-162b23, WORKING DRAFT SEQUENCE, 4
ordered pieces
Length = 117818
Score = 90.9 bits (192), Expect(3) = 7e-024
Identities = 34/48 (70%), Positives = 42/48 (87%)
Frame = -3 / +2
Query: 593 GLVASGVAFALQIWCIDRGGPLFTAVFQPVQTVAVAVMASAILGDQLY 450
G+VASG+AFA+QIWCIDRGGP+F AV+QPVQT VA+MAS LG++ Y
Sbjct: 89513 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVAIMASIALGEEFY 89656
Score = 42.8 bits (87), Expect(3) = 7e-024
Identities = 19/32 (59%), Positives = 20/32 (62%)
Frame = -3 / +2
Query: 689 LLQFLAIAAFTEEDLSRWKVRSGGELFTILYA 594
LLQFL IA E + W SGGE FTILYA
Sbjct: 89177 LLQFLLIALVFERNAQAWIFHSGGEAFTILYA 89272
Score = 33.1 bits (66), Expect(3) = 7e-024
Identities = 13/18 (72%), Positives = 14/18 (77%)
Frame = -3 / +3
Query: 407 YFVLWGKSAEKKGARNLL 354
YFVLWGKS EKK A+ L
Sbjct: 90204 YFVLWGKSEEKKFAKEQL 90257
>gb|AC159535.5| Medicago truncatula clone mth2-152f22, WORKING DRAFT SEQUENCE, 7
unordered pieces
Length = 129622
Score = 90.9 bits (192), Expect(3) = 8e-024
Identities = 34/48 (70%), Positives = 42/48 (87%)
Frame = -3 / +1
Query: 593 GLVASGVAFALQIWCIDRGGPLFTAVFQPVQTVAVAVMASAILGDQLY 450
G+VASG+AFA+QIWCIDRGGP+F AV+QPVQT VA+MAS LG++ Y
Sbjct: 95929 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVAIMASIALGEEFY 96072
Score = 42.8 bits (87), Expect(3) = 8e-024
Identities = 19/32 (59%), Positives = 20/32 (62%)
Frame = -3 / +1
Query: 689 LLQFLAIAAFTEEDLSRWKVRSGGELFTILYA 594
LLQFL IA E + W SGGE FTILYA
Sbjct: 95593 LLQFLLIALVFERNAQAWIFHSGGEAFTILYA 95688
Score = 33.1 bits (66), Expect(3) = 8e-024
Identities = 13/18 (72%), Positives = 14/18 (77%)
Frame = -3 / +2
Query: 407 YFVLWGKSAEKKGARNLL 354
YFVLWGKS EKK A+ L
Sbjct: 96620 YFVLWGKSEEKKFAKEQL 96673
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 591,376,114
Number of Sequences: 392609
Number of extensions: 7241772
Number of successful extensions: 108385
Number of sequences better than 1.0e-020: 11
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 22057602875
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)