TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 4573985.2.1
(373 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BI270588.1|BI270588 NF010B12FL1F1101 Developing flower M... 180 2e-044
gb|CG936178.1|CG936178 MBENI74TR mth2 Medicago truncatula g... 115 3e-042
gb|AC151000.3| Medicago truncatula chromosome 2 BAC clone m... 115 4e-040
gb|AC135102.16| Medicago truncatula clone mth2-8m3, complet... 115 4e-040
gb|BI308315.1|BI308315 EST529725 GPOD Medicago truncatula c... 130 1e-029
gb|AC144618.7| Medicago truncatula clone mth2-21d12, comple... 76 1e-024
emb|CT030180.3| M.truncatula DNA sequence from clone MTH2-1... 76 2e-024
gb|AJ845540.1|AJ845540 AJ845540 MtSNF Medicago truncatula c... 106 2e-022
gb|AJ845845.1|AJ845845 AJ845845 MtSNF Medicago truncatula c... 106 2e-022
gb|AW692796.1|AW692796 NF055G07ST1F1000 Developing stem Med... 86 1e-021
>gb|BI270588.1|BI270588 NF010B12FL1F1101 Developing flower Medicago truncatula cDNA clone
NF010B12FL 5', mRNA sequence
Length = 650
Score = 180 bits (387), Expect = 2e-044
Identities = 71/107 (66%), Positives = 79/107 (73%)
Frame = -3 / +2
Query: 344 IGELDHIKELIKWGTDYILKTFNSSADTIDRIVAQVGVGDTSKGSSQPNDHYCWMRPEDI 165
+G L+H+KELIKWGTDY LKTFNS+ADTI + AQVG G T + S+ PNDHYCWMRPEDI
Sbjct: 83 LGSLNHVKELIKWGTDYFLKTFNSTADTITTLAAQVGSGVTGEDSTTPNDHYCWMRPEDI 262
Query: 164 DYKRPVTECHSCSDXXXXXXXXXXXXSIVFKDSKTYSDKLVKGAKAL 24
DY RPVTECHSCSD SIVFKD+K YS KLV GA L
Sbjct: 263 DYDRPVTECHSCSDLAAEMAAALAAASIVFKDNKAYSKKLVHGATTL 403
>gb|CG936178.1|CG936178 MBENI74TR mth2 Medicago truncatula genomic clone 8M3, DNA sequence
Length = 845
Score = 115 bits (246), Expect(2) = 3e-042
Identities = 46/72 (63%), Positives = 49/72 (68%)
Frame = -3 / -3
Query: 239 VGVGDTSKGSSQPNDHYCWMRPEDIDYKRPVTECHSCSDXXXXXXXXXXXXSIVFKDSKT 60
VG G T + S+ PNDHYCWMRPEDIDY RPVTECHSCSD SIVFKD+K
Sbjct: 345 VGSGVTGEDSTTPNDHYCWMRPEDIDYDRPVTECHSCSDLAAEMAAALAAASIVFKDNKA 166
Query: 59 YSDKLVKGAKAL 24
YS KLV GA L
Sbjct: 165 YSKKLVHGATTL 130
Score = 84.5 bits (178), Expect(2) = 6e-025
Identities = 33/70 (47%), Positives = 46/70 (65%)
Frame = +3 / +1
Query: 42 DELVGVGFAVLEHYGSCSQGSSHFRR*I*ARVTLGDWPFVVDVLWSHPAVMVIRLTAAFR 221
++ +G+GF V E+ G SQGS HFR I* V DWP +V++LW+HPAV++IR F
Sbjct: 148 NKFLGIGFVVFENNGCSSQGSGHFRSKI*TTVAFSDWPVIVNILWAHPAVVIIRSRTIFP 327
Query: 222 GVTYTHLSDN 251
G + +LS N
Sbjct: 328 GNSTPNLSKN 357
Score = 78.5 bits (165), Expect(2) = 3e-042
Identities = 30/40 (75%), Positives = 35/40 (87%)
Frame = -3 / -1
Query: 356 RYEAIGELDHIKELIKWGTDYILKTFNSSADTIDRIVAQV 237
+YEA GEL+H+KELIKWGTDY LKTFNS+ADTI + AQV
Sbjct: 797 KYEATGELNHVKELIKWGTDYFLKTFNSTADTITTLAAQV 678
Score = 50.6 bits (104), Expect(2) = 6e-025
Identities = 25/40 (62%), Positives = 29/40 (72%)
Frame = +1 / +1
Query: 238 T*ATILSIVSADELKVLRM*SVPHLISSLIWSSSPMASYL 357
T A + +VSA L VLR *SVPHL+SS W SSP+ASYL
Sbjct: 679 TCAARVVMVSAVLLNVLRK*SVPHLMSSFTWLSSPVASYL 798
>gb|AC151000.3| Medicago truncatula chromosome 2 BAC clone mth2-193p1, complete
sequence
Length = 120579
Score = 115 bits (246), Expect(2) = 4e-040
Identities = 46/72 (63%), Positives = 49/72 (68%)
Frame = -3 / +3
Query: 239 VGVGDTSKGSSQPNDHYCWMRPEDIDYKRPVTECHSCSDXXXXXXXXXXXXSIVFKDSKT 60
VG G T + S+ PNDHYCWMRPEDIDY RPVTECHSCSD SIVFKD+K
Sbjct: 23217 VGSGVTGEDSTTPNDHYCWMRPEDIDYDRPVTECHSCSDLAAEMAAALAAASIVFKDNKA 23396
Query: 59 YSDKLVKGAKAL 24
YS KLV GA L
Sbjct: 23397 YSKKLVHGATTL 23432
Score = 84.5 bits (178), Expect(2) = 8e-023
Identities = 33/70 (47%), Positives = 46/70 (65%)
Frame = +3 / -2
Query: 42 DELVGVGFAVLEHYGSCSQGSSHFRR*I*ARVTLGDWPFVVDVLWSHPAVMVIRLTAAFR 221
++ +G+GF V E+ G SQGS HFR I* V DWP +V++LW+HPAV++IR F
Sbjct: 23414 NKFLGIGFVVFENNGCSSQGSGHFRSKI*TTVAFSDWPVIVNILWAHPAVVIIRSRTIFP 23235
Query: 222 GVTYTHLSDN 251
G + +LS N
Sbjct: 23234 GNSTPNLSKN 23205
Score = 78.5 bits (165), Expect(2) = 4e-040
Identities = 30/40 (75%), Positives = 35/40 (87%)
Frame = -3 / +1
Query: 356 RYEAIGELDHIKELIKWGTDYILKTFNSSADTIDRIVAQV 237
+YEA GEL+H+KELIKWGTDY LKTFNS+ADTI + AQV
Sbjct: 22765 KYEATGELNHVKELIKWGTDYFLKTFNSTADTITTLAAQV 22884
Score = 50.6 bits (104), Expect(2) = 8e-023
Identities = 25/40 (62%), Positives = 29/40 (72%)
Frame = +1 / -2
Query: 238 T*ATILSIVSADELKVLRM*SVPHLISSLIWSSSPMASYL 357
T A + +VSA L VLR *SVPHL+SS W SSP+ASYL
Sbjct: 22883 TCAARVVMVSAVLLNVLRK*SVPHLMSSFTWLSSPVASYL 22764
>gb|AC135102.16| Medicago truncatula clone mth2-8m3, complete sequence
Length = 144042
Score = 115 bits (246), Expect(2) = 4e-040
Identities = 46/72 (63%), Positives = 49/72 (68%)
Frame = -3 / -3
Query: 239 VGVGDTSKGSSQPNDHYCWMRPEDIDYKRPVTECHSCSDXXXXXXXXXXXXSIVFKDSKT 60
VG G T + S+ PNDHYCWMRPEDIDY RPVTECHSCSD SIVFKD+K
Sbjct: 361 VGSGVTGEDSTTPNDHYCWMRPEDIDYDRPVTECHSCSDLAAEMAAALAAASIVFKDNKA 182
Query: 59 YSDKLVKGAKAL 24
YS KLV GA L
Sbjct: 181 YSKKLVHGATTL 146
Score = 84.5 bits (178), Expect(2) = 9e-023
Identities = 33/70 (47%), Positives = 46/70 (65%)
Frame = +3 / +2
Query: 42 DELVGVGFAVLEHYGSCSQGSSHFRR*I*ARVTLGDWPFVVDVLWSHPAVMVIRLTAAFR 221
++ +G+GF V E+ G SQGS HFR I* V DWP +V++LW+HPAV++IR F
Sbjct: 164 NKFLGIGFVVFENNGCSSQGSGHFRSKI*TTVAFSDWPVIVNILWAHPAVVIIRSRTIFP 343
Query: 222 GVTYTHLSDN 251
G + +LS N
Sbjct: 344 GNSTPNLSKN 373
Score = 78.5 bits (165), Expect(2) = 4e-040
Identities = 30/40 (75%), Positives = 35/40 (87%)
Frame = -3 / -1
Query: 356 RYEAIGELDHIKELIKWGTDYILKTFNSSADTIDRIVAQV 237
+YEA GEL+H+KELIKWGTDY LKTFNS+ADTI + AQV
Sbjct: 813 KYEATGELNHVKELIKWGTDYFLKTFNSTADTITTLAAQV 694
Score = 50.6 bits (104), Expect(2) = 9e-023
Identities = 25/40 (62%), Positives = 29/40 (72%)
Frame = +1 / +2
Query: 238 T*ATILSIVSADELKVLRM*SVPHLISSLIWSSSPMASYL 357
T A + +VSA L VLR *SVPHL+SS W SSP+ASYL
Sbjct: 695 TCAARVVMVSAVLLNVLRK*SVPHLMSSFTWLSSPVASYL 814
>gb|BI308315.1|BI308315 EST529725 GPOD Medicago truncatula cDNA clone pGPOD-2B18 5' end,
mRNA sequence
Length = 694
Score = 130 bits (279), Expect = 1e-029
Identities = 53/84 (63%), Positives = 58/84 (69%)
Frame = -3 / +1
Query: 275 SSADTIDRIVAQVGVGDTSKGSSQPNDHYCWMRPEDIDYKRPVTECHSCSDXXXXXXXXX 96
S+ADTI + AQVG G T + S+ PNDHYCWMRPEDIDY RPVTECHSCSD
Sbjct: 1 STADTITTLAAQVGSGVTGEDSTTPNDHYCWMRPEDIDYDRPVTECHSCSDLAAEMAAAL 180
Query: 95 XXXSIVFKDSKTYSDKLVKGAKAL 24
SIVFKD+K YS KLV GA L
Sbjct: 181 AAASIVFKDNKAYSKKLVHGATTL 252
>gb|AC144618.7| Medicago truncatula clone mth2-21d12, complete sequence
Length = 106554
Score = 75.8 bits (159), Expect(2) = 1e-024
Identities = 31/68 (45%), Positives = 36/68 (52%)
Frame = -3 / -3
Query: 233 VGDTSKGSSQPNDHYCWMRPEDIDYKRPVTECHSCSDXXXXXXXXXXXXSIVFKDSKTYS 54
VG + GS P+DHYCW +PED+ Y RP T D SIVF D YS
Sbjct: 91837 VGGSLNGSRTPDDHYCWQKPEDMSYPRPTTTVFEGPDLAGEMAAALAAASIVFLDDAAYS 91658
Query: 53 DKLVKGAK 30
KLVKGA+
Sbjct: 91657 KKLVKGAE 91634
Score = 65.7 bits (137), Expect(2) = 1e-024
Identities = 25/42 (59%), Positives = 33/42 (78%)
Frame = -3 / -2
Query: 362 RPRYEAIGELDHIKELIKWGTDYILKTFNSSADTIDRIVAQV 237
+ +Y A+ E +H +ELIKWGTDY+L TFNSSA I++I AQV
Sbjct: 92204 KQKYMAVNEYNHARELIKWGTDYLLLTFNSSASKINKIYAQV 92079
>emb|CT030180.3| M.truncatula DNA sequence from clone MTH2-177C12 on chromosome 3,
complete sequence
Length = 141577
Score = 75.8 bits (159), Expect(2) = 2e-024
Identities = 31/68 (45%), Positives = 36/68 (52%)
Frame = -3 / +1
Query: 233 VGDTSKGSSQPNDHYCWMRPEDIDYKRPVTECHSCSDXXXXXXXXXXXXSIVFKDSKTYS 54
VG + GS P+DHYCW +PED+ Y RP T D SIVF D YS
Sbjct: 134770 VGGSLNGSRTPDDHYCWQKPEDMSYPRPTTTVFEGPDLAGEMAAALAAASIVFLDDAAYS 134949
Query: 53 DKLVKGAK 30
KLVKGA+
Sbjct: 134950 KKLVKGAE 134973
Score = 65.7 bits (137), Expect(2) = 2e-024
Identities = 25/42 (59%), Positives = 33/42 (78%)
Frame = -3 / +3
Query: 362 RPRYEAIGELDHIKELIKWGTDYILKTFNSSADTIDRIVAQV 237
+ +Y A+ E +H +ELIKWGTDY+L TFNSSA I++I AQV
Sbjct: 134403 KQKYMAVNEYNHARELIKWGTDYLLLTFNSSASKINKIYAQV 134528
>gb|AJ845540.1|AJ845540 AJ845540 MtSNF Medicago truncatula cDNA clone MtNF06M12N1, mRNA
sequence
Length = 295
Score = 106 bits (226), Expect = 2e-022
Identities = 41/60 (68%), Positives = 42/60 (70%)
Frame = -3 / -1
Query: 203 PNDHYCWMRPEDIDYKRPVTECHSCSDXXXXXXXXXXXXSIVFKDSKTYSDKLVKGAKAL 24
PNDHYCWMRPEDIDY RPVTECHSCSD SIVFKD+K YS KLV GA L
Sbjct: 289 PNDHYCWMRPEDIDYDRPVTECHSCSDLAAEMAAALAAASIVFKDNKAYSKKLVHGATTL 110
>gb|AJ845845.1|AJ845845 AJ845845 MtSNF Medicago truncatula cDNA clone MtNF11L17N1, mRNA
sequence
Length = 298
Score = 106 bits (226), Expect = 2e-022
Identities = 41/60 (68%), Positives = 42/60 (70%)
Frame = -3 / -2
Query: 203 PNDHYCWMRPEDIDYKRPVTECHSCSDXXXXXXXXXXXXSIVFKDSKTYSDKLVKGAKAL 24
PNDHYCWMRPEDIDY RPVTECHSCSD SIVFKD+K YS KLV GA L
Sbjct: 288 PNDHYCWMRPEDIDYDRPVTECHSCSDLAAEMAAALAAASIVFKDNKAYSKKLVHGATTL 109
>gb|AW692796.1|AW692796 NF055G07ST1F1000 Developing stem Medicago truncatula cDNA clone
NF055G07ST 5', mRNA sequence
Length = 664
Score = 85.8 bits (181), Expect(3) = 2e-021
Identities = 34/49 (69%), Positives = 40/49 (81%)
Frame = -3 / +1
Query: 356 RYEAIGELDHIKELIKWGTDYILKTFNSSADTIDRIVAQVGVGDTSKGS 210
+YEA GEL+H+KELIKWGTDY LKTFNS+ADTI + AQVG G T + S
Sbjct: 346 KYEATGELNHVKELIKWGTDYFLKTFNSTADTITTLAAQVGSGVTGEDS 492
Score = 58.8 bits (122), Expect(3) = 1e-021
Identities = 28/45 (62%), Positives = 33/45 (73%)
Frame = +1 / -3
Query: 223 VSPTPT*ATILSIVSADELKVLRM*SVPHLISSLIWSSSPMASYL 357
V+P PT A + +VSA L VLR *SVPHL+SS W SSP+ASYL
Sbjct: 479 VTPLPTCAARVVMVSAVLLNVLRK*SVPHLMSSFTWLSSPVASYL 345
Score = 44.1 bits (90), Expect(3) = 1e-021
Identities = 13/23 (56%), Positives = 19/23 (82%)
Frame = +3 / -1
Query: 135 VTLGDWPFVVDVLWSHPAVMVIR 203
V DWP +V++LW+HPAV++IR
Sbjct: 568 VAFSDWPVIVNILWAHPAVVIIR 500
Score = 37.3 bits (75), Expect(3) = 1e-021
Identities = 15/32 (46%), Positives = 22/32 (68%)
Frame = +3 / -2
Query: 42 DELVGVGFAVLEHYGSCSQGSSHFRR*I*ARV 137
++ +G+GF V E+ G SQGS HFR I* ++
Sbjct: 663 NKFLGIGFVVFENNGCSSQGSGHFRSKI*KQL 568
Score = 30.4 bits (60), Expect(3) = 2e-021
Identities = 14/29 (48%), Positives = 15/29 (51%)
Frame = -3 / +1
Query: 128 SDXXXXXXXXXXXXSIVFKDSKTYSDKLV 42
SD SIVFKD+K YS KLV
Sbjct: 577 SDLAAEMAAALAAASIVFKDNKAYSKKLV 663
Score = 23.5 bits (45), Expect(3) = 2e-021
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = -1 / +3
Query: 202 LMTITAG*DQRTSTTKGQSPSVTL 131
LM TAG QR T GQS + T+
Sbjct: 501 LMITTAGCAQRILTMTGQSLNATV 572
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 298,323,844
Number of Sequences: 392609
Number of extensions: 3333655
Number of successful extensions: 67827
Number of sequences better than 1.0e-020: 11
length of database: 147,244,331
effective HSP length: 52
effective length of database: 126,828,663
effective search space used: 9004835073
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)