TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3866402.2.1
(406 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
emb|CR342893.1| mte1-75J4FM1 BAC end, cultivar Jemalong A17... 159 2e-038
gb|AC158209.13| Medicago truncatula clone mth2-154h5, compl... 159 2e-038
gb|BQ123088.1|BQ123088 EST608664 GLSD Medicago truncatula c... 151 4e-036
gb|AL381590.1|AL381590 MtBC01H02R2 MtBC Medicago truncatula... 151 6e-036
gb|AW688473.2|AW688473 NF008A08ST1F1000 Developing stem Med... 148 5e-035
gb|AC152184.1| Medicago truncatula chromosome 7 BAC clone m... 141 4e-033
gb|AC122723.6| Medicago truncatula clone mth2-5i15, complet... 141 4e-033
emb|CR500324.1| mth2-177H7FM1 BAC end, cultivar Jemalong A1... 138 6e-032
emb|CR320381.1| mte1-46O24FM1 BAC end, cultivar Jemalong A1... 138 6e-032
gb|CG920826.1|CG920826 MBECG06TF mth2 Medicago truncatula g... 135 4e-031
gb|AC137603.16| Medicago truncatula clone mth2-14b10, compl... 135 4e-031
gb|AC152887.20| Medicago truncatula clone mth2-91j4, WORKIN... 123 1e-027
gb|AC157648.18| Medicago truncatula clone mth2-68d9, comple... 123 1e-027
gb|AL389186.1|AL389186 MtBC53C09R1 MtBC Medicago truncatula... 122 1e-026
emb|CR493732.1| mth2-143K5FM1 BAC end, cultivar Jemalong A1... 119 3e-026
gb|CX533175.1|CX533175 s13dNF0CG12MJ088_319513 Methyl Jasmo... 117 9e-026
gb|AC170583.6| Medicago truncatula clone mth2-19b5, complet... 108 5e-023
gb|AL381589.1|AL381589 MtBC01H02F3 MtBC Medicago truncatula... 107 1e-022
>emb|CR342893.1| mte1-75J4FM1 BAC end, cultivar Jemalong A17 of Medicago truncatula,
genomic survey sequence
Length = 724
Score = 159 bits (342), Expect = 2e-038
Identities = 64/78 (82%), Positives = 72/78 (92%)
Frame = -3 / -1
Query: 386 QISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARLLFNQAF 207
QI+QA +PV+KA G+WGSVKALSRGYEYLMG+VIF PVA+LAWFPFVSEFQ RLL+NQAF
Sbjct: 379 QIAQACRPVVKAIGMWGSVKALSRGYEYLMGVVIFTPVAILAWFPFVSEFQTRLLYNQAF 200
Query: 206 SRGLQISRILAGGKKQR* 153
SRGLQI RILAGGKK +*
Sbjct: 199 SRGLQIQRILAGGKKNK* 146
Score = 110 bits (235), Expect = 1e-023
Identities = 51/83 (61%), Positives = 58/83 (69%)
Frame = +1 / +3
Query: 154 YLCFLPPAKIREI*RPRLNA*LKSNLAWNSETNGNHASTATGTKMTIPIRYSYPLERAFT 333
YL FLPPA+IR I* P LNA*L S+L NSETNGN A+ ATG K+T PIRYSYPL++A T
Sbjct: 147 YLFFLPPARIRWI*SPLLNA*LYSSLV*NSETNGNQANMATGVKITTPIRYSYPLDKALT 326
Query: 334 DPQSPNALMTGLLA*DICRMAHP 402
+P P A TG A IC P
Sbjct: 327 EPHIPMAFTTGRHACAICSCIMP 395
>gb|AC158209.13| Medicago truncatula clone mth2-154h5, complete sequence
Length = 93864
Score = 159 bits (342), Expect = 2e-038
Identities = 64/78 (82%), Positives = 72/78 (92%)
Frame = -3 / -3
Query: 386 QISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARLLFNQAF 207
QI+QA +PV+KA G+WGSVKALSRGYEYLMG+VIF PVA+LAWFPFVSEFQ RLL+NQAF
Sbjct: 54331 QIAQACRPVVKAIGMWGSVKALSRGYEYLMGVVIFTPVAILAWFPFVSEFQTRLLYNQAF 54152
Query: 206 SRGLQISRILAGGKKQR* 153
SRGLQI RILAGGKK +*
Sbjct: 54151 SRGLQIQRILAGGKKNK* 54098
Score = 110 bits (235), Expect = 1e-023
Identities = 51/83 (61%), Positives = 58/83 (69%)
Frame = +1 / +3
Query: 154 YLCFLPPAKIREI*RPRLNA*LKSNLAWNSETNGNHASTATGTKMTIPIRYSYPLERAFT 333
YL FLPPA+IR I* P LNA*L S+L NSETNGN A+ ATG K+T PIRYSYPL++A T
Sbjct: 54099 YLFFLPPARIRWI*SPLLNA*LYSSLV*NSETNGNQANMATGVKITTPIRYSYPLDKALT 54278
Query: 334 DPQSPNALMTGLLA*DICRMAHP 402
+P P A TG A IC P
Sbjct: 54279 EPHIPMAFTTGRHACAICSCIMP 54347
>gb|BQ123088.1|BQ123088 EST608664 GLSD Medicago truncatula cDNA clone pGLSD-31G15, mRNA
sequence
Length = 673
Score = 151 bits (325), Expect = 4e-036
Identities = 60/83 (72%), Positives = 71/83 (85%)
Frame = -3 / +2
Query: 404 TGWAILQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARL 225
TGW +LQI+QA KP+I GLWGSV+AL+RGYE +MG+++F PVA LAWFPFVSEFQ R+
Sbjct: 278 TGWGMLQIAQACKPLIAKTGLWGSVRALARGYEVIMGLLLFTPVAFLAWFPFVSEFQTRM 457
Query: 224 LFNQAFSRGLQISRILAGGKKQR 156
LFNQAFSRGLQISRIL G K+ R
Sbjct: 458 LFNQAFSRGLQISRILGGQKRDR 526
>gb|AL381590.1|AL381590 MtBC01H02R2 MtBC Medicago truncatula cDNA clone MtBC01H02 T7, mRNA
sequence
Length = 548
Score = 151 bits (324), Expect = 6e-036
Identities = 58/83 (69%), Positives = 71/83 (85%)
Frame = -3 / +2
Query: 404 TGWAILQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARL 225
TGW +LQI+QA KP+++ G WGSVK L+RGYE +MG+++F PVA LAWFPFVSEFQ R+
Sbjct: 128 TGWGMLQIAQALKPIVRRAGFWGSVKTLARGYEIVMGLLLFTPVAFLAWFPFVSEFQTRM 307
Query: 224 LFNQAFSRGLQISRILAGGKKQR 156
LFNQAFSRGLQISRIL G +K+R
Sbjct: 308 LFNQAFSRGLQISRILGGQRKER 376
>gb|AW688473.2|AW688473 NF008A08ST1F1000 Developing stem Medicago truncatula cDNA clone
NF008A08ST 5', mRNA sequence
Length = 788
Score = 148 bits (317), Expect = 5e-035
Identities = 57/83 (68%), Positives = 70/83 (84%)
Frame = -3 / +2
Query: 404 TGWAILQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARL 225
TGW +LQI+QA KP+I GLWGSV+ L+RGYE +MG+++F P+A LAWFPFVSEFQ R+
Sbjct: 437 TGWGMLQIAQACKPLIAKTGLWGSVRXLARGYEVIMGLLLFTPIAFLAWFPFVSEFQTRM 616
Query: 224 LFNQAFSRGLQISRILAGGKKQR 156
LFNQAFS+GLQISRIL G K+ R
Sbjct: 617 LFNQAFSKGLQISRILGGQKRDR 685
>gb|AC152184.1| Medicago truncatula chromosome 7 BAC clone mte1-61c3, complete sequence
Length = 103090
Score = 141 bits (303), Expect = 4e-033
Identities = 55/79 (69%), Positives = 68/79 (86%)
Frame = -3 / +1
Query: 392 ILQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARLLFNQ 213
+LQI+QA KP+++ G WGSVK L+RGYE +MG+++F PVA LAWFPFVSEFQ R+LFNQ
Sbjct: 9931 LLQIAQALKPIVRRAGFWGSVKTLARGYEIVMGLLLFTPVAFLAWFPFVSEFQTRMLFNQ 10110
Query: 212 AFSRGLQISRILAGGKKQR 156
AFSRGLQISRIL G +K+R
Sbjct: 10111 AFSRGLQISRILGGQRKER 10167
>gb|AC122723.6| Medicago truncatula clone mth2-5i15, complete sequence
Length = 105353
Score = 141 bits (303), Expect = 4e-033
Identities = 55/79 (69%), Positives = 68/79 (86%)
Frame = -3 / +1
Query: 392 ILQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARLLFNQ 213
+LQI+QA KP+++ G WGSVK L+RGYE +MG+++F PVA LAWFPFVSEFQ R+LFNQ
Sbjct: 81328 LLQIAQALKPIVRRAGFWGSVKTLARGYEIVMGLLLFTPVAFLAWFPFVSEFQTRMLFNQ 81507
Query: 212 AFSRGLQISRILAGGKKQR 156
AFSRGLQISRIL G +K+R
Sbjct: 81508 AFSRGLQISRILGGQRKER 81564
>emb|CR500324.1| mth2-177H7FM1 BAC end, cultivar Jemalong A17 of Medicago
truncatula, genomic survey sequence
Length = 638
Score = 138 bits (295), Expect = 6e-032
Identities = 55/78 (70%), Positives = 67/78 (85%)
Frame = -3 / -1
Query: 389 LQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARLLFNQA 210
++I+QA KP+I GLWGSV+AL+RGYE +MG+++F PVA LAWFPFVSEFQ R+LFNQA
Sbjct: 617 VKIAQACKPLIAKTGLWGSVRALARGYEVIMGLLLFTPVAFLAWFPFVSEFQTRMLFNQA 438
Query: 209 FSRGLQISRILAGGKKQR 156
FSRGLQISRIL G K+ R
Sbjct: 437 FSRGLQISRILGGQKRDR 384
>emb|CR320381.1| mte1-46O24FM1 BAC end, cultivar Jemalong A17 of Medicago
truncatula, genomic survey sequence
Length = 888
Score = 138 bits (295), Expect = 6e-032
Identities = 55/78 (70%), Positives = 67/78 (85%)
Frame = -3 / -1
Query: 389 LQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARLLFNQA 210
++I+QA KP+I GLWGSV+AL+RGYE +MG+++F PVA LAWFPFVSEFQ R+LFNQA
Sbjct: 762 VKIAQACKPLIAKTGLWGSVRALARGYEVIMGLLLFTPVAFLAWFPFVSEFQTRMLFNQA 583
Query: 209 FSRGLQISRILAGGKKQR 156
FSRGLQISRIL G K+ R
Sbjct: 582 FSRGLQISRILGGQKRDR 529
>gb|CG920826.1|CG920826 MBECG06TF mth2 Medicago truncatula genomic clone 25B11, DNA
sequence
Length = 802
Score = 135 bits (289), Expect = 4e-031
Identities = 54/79 (68%), Positives = 66/79 (83%)
Frame = -3 / -1
Query: 392 ILQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARLLFNQ 213
I+QI+QA KP+++ G W SVK L+RGYE +MG+++F PVA LAWFPFVSEFQ R+LFNQ
Sbjct: 700 IMQIAQALKPLVRRGGFWESVKTLARGYEVIMGLLLFTPVAFLAWFPFVSEFQTRMLFNQ 521
Query: 212 AFSRGLQISRILAGGKKQR 156
AFSRGLQISRIL G +K R
Sbjct: 520 AFSRGLQISRILGGQRKGR 464
>gb|AC137603.16| Medicago truncatula clone mth2-14b10, complete sequence
Length = 109793
Score = 135 bits (289), Expect = 4e-031
Identities = 54/79 (68%), Positives = 66/79 (83%)
Frame = -3 / -3
Query: 392 ILQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARLLFNQ 213
I+QI+QA KP+++ G W SVK L+RGYE +MG+++F PVA LAWFPFVSEFQ R+LFNQ
Sbjct: 60207 IMQIAQALKPLVRRGGFWESVKTLARGYEVIMGLLLFTPVAFLAWFPFVSEFQTRMLFNQ 60028
Query: 212 AFSRGLQISRILAGGKKQR 156
AFSRGLQISRIL G +K R
Sbjct: 60027 AFSRGLQISRILGGQRKGR 59971
>gb|AC152887.20| Medicago truncatula clone mth2-91j4, WORKING DRAFT SEQUENCE, 2 ordered
pieces
Length = 116277
Score = 123 bits (263), Expect = 1e-027
Identities = 50/75 (66%), Positives = 62/75 (82%)
Frame = -3 / -1
Query: 389 LQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARLLFNQA 210
++I+Q + ++K LW SV+ LSR YEY+MG++IF+P AVL+WFPFVSEFQ RLLFNQA
Sbjct: 96009 IKIAQTCRGLLKWAKLWASVRELSRAYEYVMGLIIFMPTAVLSWFPFVSEFQTRLLFNQA 95830
Query: 209 FSRGLQISRILAGGK 165
FSRGLQIS ILAG K
Sbjct: 95829 FSRGLQISMILAGKK 95785
>gb|AC157648.18| Medicago truncatula clone mth2-68d9, complete sequence
Length = 84361
Score = 123 bits (263), Expect = 1e-027
Identities = 50/75 (66%), Positives = 62/75 (82%)
Frame = -3 / +2
Query: 389 LQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARLLFNQA 210
++I+Q + ++K LW SV+ LSR YEY+MG++IF+P AVL+WFPFVSEFQ RLLFNQA
Sbjct: 77825 IKIAQTCRGLLKWAKLWASVRELSRAYEYVMGLIIFMPTAVLSWFPFVSEFQTRLLFNQA 78004
Query: 209 FSRGLQISRILAGGK 165
FSRGLQIS ILAG K
Sbjct: 78005 FSRGLQISMILAGKK 78049
>gb|AL389186.1|AL389186 MtBC53C09R1 MtBC Medicago truncatula cDNA clone MtBC53C09 T7, mRNA
sequence
Length = 544
Score = 122 bits (260), Expect(2) = 1e-026
Identities = 49/78 (62%), Positives = 62/78 (79%)
Frame = -3 / +1
Query: 404 TGWAILQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARL 225
TGW IL I+ A KPV+K GLW +++L+R Y+ MG++IFVP+A +WFPFVS FQ RL
Sbjct: 124 TGWGILSIAAAWKPVMKRLGLWKFIRSLARLYDAGMGMLIFVPIAFFSWFPFVSTFQTRL 303
Query: 224 LFNQAFSRGLQISRILAG 171
+FNQAFSRGL+IS ILAG
Sbjct: 304 MFNQAFSRGLEISLILAG 357
Score = 17.6 bits (32), Expect(2) = 1e-026
Identities = 5/12 (41%), Positives = 6/12 (50%)
Frame = -2 / +2
Query: 90 CTWVMGVCWRNC 55
CTW+M C
Sbjct: 383 CTWLMPYTESKC 418
>emb|CR493732.1| mth2-143K5FM1 BAC end, cultivar Jemalong A17 of Medicago
truncatula, genomic survey sequence
Length = 687
Score = 119 bits (254), Expect = 3e-026
Identities = 48/57 (84%), Positives = 52/57 (91%)
Frame = -3 / +2
Query: 326 ALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARLLFNQAFSRGLQISRILAGGKKQR 156
AL+RGYEYLM +VIF PVA+LAWFPFVSEFQ RLLFNQAFSRGLQI RILAGGKK +
Sbjct: 2 ALARGYEYLMAVVIFTPVAILAWFPFVSEFQTRLLFNQAFSRGLQIQRILAGGKKHK 172
>gb|CX533175.1|CX533175 s13dNF0CG12MJ088_319513 Methyl Jasmonate-Elicited Root Cell
Suspension Culture Medicago truncatula cDNA, mRNA
sequence
Length = 611
Score = 117 bits (250), Expect = 9e-026
Identities = 46/80 (57%), Positives = 62/80 (77%)
Frame = -3 / +2
Query: 404 TGWAILQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARL 225
TGW IL ++ K ++K+ GLW SV+ +R Y+ MG++IF P+A L+WFPF+S FQ+RL
Sbjct: 95 TGWGILSLAITWKGIMKSLGLWESVREFARMYDAGMGMIIFSPIAFLSWFPFISTFQSRL 274
Query: 224 LFNQAFSRGLQISRILAGGK 165
LFNQAFSRGL+IS IL+G K
Sbjct: 275 LFNQAFSRGLEISLILSGNK 334
>gb|AC170583.6| Medicago truncatula clone mth2-19b5, complete sequence
Length = 113632
Score = 108 bits (230), Expect = 5e-023
Identities = 44/71 (61%), Positives = 57/71 (80%)
Frame = -3 / +2
Query: 383 ISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARLLFNQAFS 204
I+ A KPV+K GLW +++L+R Y+ MG++IFVP+A +WFPFVS FQ RL+FNQAFS
Sbjct: 16565 IAAAWKPVMKRLGLWKFIRSLARLYDAGMGMLIFVPIAFFSWFPFVSTFQTRLMFNQAFS 16744
Query: 203 RGLQISRILAG 171
RGL+IS ILAG
Sbjct: 16745 RGLEISLILAG 16777
>gb|AL381589.1|AL381589 MtBC01H02F3 MtBC Medicago truncatula cDNA clone MtBC01H02 T3, mRNA
sequence
Length = 502
Score = 107 bits (228), Expect = 1e-022
Identities = 39/59 (66%), Positives = 49/59 (83%)
Frame = -3 / +2
Query: 404 TGWAILQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQAR 228
TGW +LQI+QA KP+++ G WGSVK L+RGYE +MG+++F PVA LAWFPFVSEFQ R
Sbjct: 326 TGWGMLQIAQALKPIVRRAGFWGSVKTLARGYEIVMGLLLFTPVAFLAWFPFVSEFQTR 502
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 410,296,496
Number of Sequences: 392609
Number of extensions: 5236499
Number of successful extensions: 82900
Number of sequences better than 1.0e-020: 20
length of database: 147,244,331
effective HSP length: 52
effective length of database: 126,828,663
effective search space used: 10399950366
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)