TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3848982.2.1
(697 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG453428.1|BG453428 NF091G11LF1F1085 Developing leaf Med... 116 8e-042
gb|CA917715.1|CA917715 EST641862 GPOD Medicago truncatula c... 115 7e-025
gb|AL379968.1|AL379968 MtBB48E05F1 MtBB Medicago truncatula... 114 1e-024
gb|BG455193.1|BG455193 NF100G04PL1F1034 Phosphate starved l... 114 1e-024
gb|BF648216.1|BF648216 NF045D01EC1F1012 Elicited cell cultu... 77 9e-024
gb|AW687498.2|AW687498 NF010C04RT1F1033 Developing root Med... 84 2e-023
gb|AL379969.1|AL379969 MtBB48E05R1 MtBB Medicago truncatula... 79 2e-022
gb|AC148226.7| Medicago truncatula clone mth2-5i20, complet... 67 4e-022
gb|AC174309.3| Medicago truncatula clone mth2-139k12, WORKI... 67 7e-022
>gb|BG453428.1|BG453428 NF091G11LF1F1085 Developing leaf Medicago truncatula cDNA clone
NF091G11LF 5', mRNA sequence
Length = 638
Score = 116 bits (249), Expect(3) = 8e-042
Identities = 46/84 (54%), Positives = 60/84 (71%)
Frame = +3 / +3
Query: 30 CGQVEPVHFDLSGTAFGAMAKPGQADQLRNAGRLQVQYIRVACNWNGLDIAFRVDAGSNA 209
CG HFDLSG+AFG+MA G+ ++LRNAG++ +++ RV CN+ G IAF VD+GSN
Sbjct: 6 CGSDAEYHFDLSGSAFGSMAVSGEDEELRNAGKIVIEHRRVQCNYGGSTIAFHVDSGSNQ 185
Query: 210 NYLAMAIEYESGDGDLRAXELKQS 281
Y A IEYE GDGDL ELK++
Sbjct: 186 EYFAALIEYEEGDGDLNKVELKEA 257
Score = 57.4 bits (119), Expect(3) = 8e-042
Identities = 21/30 (70%), Positives = 26/30 (86%)
Frame = +3 / +3
Query: 381 TSGSGRVLVASNVIPAGWQPGRTYRSVVNF 470
T SG+ +VA NVIPAGW+PG+TYRS+VNF
Sbjct: 360 TLKSGKTIVAHNVIPAGWKPGQTYRSIVNF 449
Score = 35.9 bits (72), Expect(3) = 8e-042
Identities = 10/21 (47%), Positives = 17/21 (80%)
Frame = +3 / +3
Query: 285 AAAWAPMQRSWGAVWQYKSGS 347
+++W MQ+SWGAVW++ G+
Sbjct: 264 SSSWDTMQQSWGAVWKFDKGA 326
>gb|CA917715.1|CA917715 EST641862 GPOD Medicago truncatula cDNA clone GPOD-37E9, mRNA
sequence
Length = 769
Score = 115 bits (246), Expect = 7e-025
Identities = 43/93 (46%), Positives = 63/93 (67%)
Frame = +3 / +2
Query: 51 HFDLSGTAFGAMAKPGQADQLRNAGRLQVQYIRVACNWNGLDIAFRVDAGSNANYLAMAI 230
HFDLSG AFG MA G+ QLRN G +QV Y R C + G +IAF+V+ GS +L++ +
Sbjct: 458 HFDLSGAAFGHMAVHGENGQLRNRGEIQVLYRRTTCVYAGKNIAFQVNEGSTPFWLSLLV 637
Query: 231 EYESGDGDLRAXELKQSGAAAWAPMQRSWGAVW 329
E+E GDGD+ + ++++G++ W M WGA+W
Sbjct: 638 EFEDGDGDISSMHIQEAGSSEWLQMNHLWGAIW 736
>gb|AL379968.1|AL379968 MtBB48E05F1 MtBB Medicago truncatula cDNA clone MtBB48E05 T3, mRNA
sequence
Length = 476
Score = 114 bits (244), Expect = 1e-024
Identities = 44/98 (44%), Positives = 64/98 (65%)
Frame = +3 / +2
Query: 51 HFDLSGTAFGAMAKPGQADQLRNAGRLQVQYIRVACNWNGLDIAFRVDAGSNANYLAMAI 230
HFDLSG AFG MA G+ QLRN G +QV Y R C + G +IAF+V+ GS +L++ +
Sbjct: 134 HFDLSGAAFGHMAVHGENGQLRNRGEIQVLYRRTTCVYAGKNIAFQVNEGSTPFWLSLLV 313
Query: 231 EYESGDGDLRAXELKQSGAAAWAPMQRSWGAVWQYKSG 344
E+E GDGD+ + ++++G++ W M WGA W +G
Sbjct: 314 EFEDGDGDISSMHIQEAGSSEWLQMNHLWGANWNIVTG 427
>gb|BG455193.1|BG455193 NF100G04PL1F1034 Phosphate starved leaf Medicago truncatula cDNA
clone NF100G04PL 5', mRNA sequence
Length = 654
Score = 114 bits (244), Expect = 1e-024
Identities = 44/98 (44%), Positives = 64/98 (65%)
Frame = +3 / +2
Query: 51 HFDLSGTAFGAMAKPGQADQLRNAGRLQVQYIRVACNWNGLDIAFRVDAGSNANYLAMAI 230
HFDLSG AFG MA G+ QLRN G +QV Y R C + G +IAF+V+ GS +L++ +
Sbjct: 206 HFDLSGAAFGHMAVHGENGQLRNRGEIQVLYRRTTCVYAGKNIAFQVNEGSTPFWLSLLV 385
Query: 231 EYESGDGDLRAXELKQSGAAAWAPMQRSWGAVWQYKSG 344
E+E GDGD+ + ++++G++ W M WGA W +G
Sbjct: 386 EFEDGDGDISSMHIQEAGSSEWLQMNHLWGANWNIVTG 499
>gb|BF648216.1|BF648216 NF045D01EC1F1012 Elicited cell culture Medicago truncatula cDNA
clone NF045D01EC 5', mRNA sequence
Length = 596
Score = 76.7 bits (161), Expect(3) = 9e-024
Identities = 28/79 (35%), Positives = 46/79 (58%)
Frame = +3 / +3
Query: 93 PGQADQLRNAGRLQVQYIRVACNWNGLDIAFRVDAGSNANYLAMAIEYESGDGDLRAXEL 272
P + LRN G++ V Y R C + G IAF V+ GS +L++ +E+E +GD+ +
Sbjct: 36 PVRTVNLRNRGQIPVIYRRTPCKYPGKKIAFHVNEGSTPFWLSLLVEFEDAEGDIGTMHI 215
Query: 273 KQSGAAAWAPMQRSWGAVW 329
+++G++ W M WGA W
Sbjct: 216 RENGSSEWLQMNHLWGANW 272
Score = 49.6 bits (102), Expect(3) = 9e-024
Identities = 19/39 (48%), Positives = 27/39 (69%)
Frame = +3 / +3
Query: 354 LQAPLSVRLTSGSGRVLVASNVIPAGWQPGRTYRSVVNF 470
L+ P SV+L+S +GR L A +V+P+ W P TY S +NF
Sbjct: 291 LRGPFSVKLSSSTGRTLSARDVVPSNWVPKATYTSRLNF 407
Score = 22.6 bits (43), Expect(3) = 9e-024
Identities = 10/17 (58%), Positives = 11/17 (64%)
Frame = +3 / +2
Query: 60 LSGTAFGAMAKPGQADQ 110
LSG AFG MA G+ Q
Sbjct: 2 LSGAAFGHMAISGENGQ 52
>gb|AW687498.2|AW687498 NF010C04RT1F1033 Developing root Medicago truncatula cDNA clone
NF010C04RT 5', mRNA sequence
Length = 522
Score = 84.0 bits (177), Expect(2) = 2e-023
Identities = 30/79 (37%), Positives = 50/79 (63%)
Frame = +3 / +2
Query: 108 QLRNAGRLQVQYIRVACNWNGLDIAFRVDAGSNANYLAMAIEYESGDGDLRAXELKQSGA 287
QLRN G + V Y R C + G ++AF+V+ GS +L++ +E+E GDGD+ + ++++G+
Sbjct: 116 QLRNRGEIPVLYRRTTCVYAGKNLAFQVNEGSTPYWLSLLVEFEDGDGDISSMYIQEAGS 295
Query: 288 AAWAPMQRSWGAVWQYKSG 344
+ W M WGA W +G
Sbjct: 296 SEWLQMNHLWGANWNIVTG 352
Score = 46.0 bits (94), Expect(2) = 2e-023
Identities = 16/39 (41%), Positives = 26/39 (66%)
Frame = +3 / +2
Query: 354 LQAPLSVRLTSGSGRVLVASNVIPAGWQPGRTYRSVVNF 470
L+ P SV+L++ +G+ + +VIP+ W P TY S +NF
Sbjct: 356 LRGPFSVKLSTSTGKSITVKDVIPSNWSPKSTYTSRLNF 472
>gb|AL379969.1|AL379969 MtBB48E05R1 MtBB Medicago truncatula cDNA clone MtBB48E05 T7, mRNA
sequence
Length = 488
Score = 79.4 bits (167), Expect(2) = 2e-022
Identities = 28/74 (37%), Positives = 47/74 (63%)
Frame = +3 / +2
Query: 123 GRLQVQYIRVACNWNGLDIAFRVDAGSNANYLAMAIEYESGDGDLRAXELKQSGAAAWAP 302
G +QV Y R C + G +IAF+V+ GS +L++ +E+E GDGD+ + ++++G++ W
Sbjct: 2 GEIQVLYRRTTCVYAGKNIAFQVNEGSTPFWLSLLVEFEDGDGDISSMHIQEAGSSEWLQ 181
Query: 303 MQRSWGAVWQYKSG 344
M WGA W +G
Sbjct: 182 MNHLWGANWNIVTG 223
Score = 47.8 bits (98), Expect(2) = 2e-022
Identities = 18/39 (46%), Positives = 27/39 (69%)
Frame = +3 / +2
Query: 354 LQAPLSVRLTSGSGRVLVASNVIPAGWQPGRTYRSVVNF 470
L+ P SV+L++ +G+ L A +VIP+ W P TY S +NF
Sbjct: 227 LRGPFSVKLSTSTGKSLTAKDVIPSHWSPKSTYTSRLNF 343
>gb|AC148226.7| Medicago truncatula clone mth2-5i20, complete sequence
Length = 84259
Score = 67.0 bits (140), Expect(3) = 4e-022
Identities = 24/40 (60%), Positives = 31/40 (77%)
Frame = +3 / +1
Query: 30 CGQVEPVHFDLSGTAFGAMAKPGQADQLRNAGRLQVQYIR 149
C + E HFDLSGTAFG++A P QAD LRNAG++ ++Y R
Sbjct: 65590 CPECESAHFDLSGTAFGSLANPNQADNLRNAGKINIRYTR 65709
Score = 50.1 bits (103), Expect(3) = 4e-022
Identities = 17/29 (58%), Positives = 22/29 (75%)
Frame = +3 / +3
Query: 384 SGSGRVLVASNVIPAGWQPGRTYRSVVNF 470
+GS ++A NVIP WQPG+ YRS+VNF
Sbjct: 70653 NGSNNTIIAENVIPQNWQPGQVYRSLVNF 70739
Score = 42.8 bits (87), Expect(3) = 4e-022
Identities = 19/38 (50%), Positives = 23/38 (60%)
Frame = +3 / +1
Query: 168 GLDIAFRVDAGSNANYLAMAIEYESGDGDLRAXELKQS 281
G I F +D G+N A IEYE+G+GDL EL QS
Sbjct: 65848 GNSIVFTIDNGANPYCFATEIEYENGNGDLVQVELSQS 65961
>gb|AC174309.3| Medicago truncatula clone mth2-139k12, WORKING DRAFT SEQUENCE, 2
unordered pieces
Length = 111023
Score = 67.0 bits (140), Expect(3) = 7e-022
Identities = 24/40 (60%), Positives = 31/40 (77%)
Frame = +3 / +2
Query: 30 CGQVEPVHFDLSGTAFGAMAKPGQADQLRNAGRLQVQYIR 149
C + E HFDLSGTAFG++A P QAD LRNAG++ ++Y R
Sbjct: 92363 CPECESAHFDLSGTAFGSLANPNQADNLRNAGKINIRYTR 92482
Score = 50.1 bits (103), Expect(3) = 7e-022
Identities = 17/29 (58%), Positives = 22/29 (75%)
Frame = +3 / +1
Query: 384 SGSGRVLVASNVIPAGWQPGRTYRSVVNF 470
+GS ++A NVIP WQPG+ YRS+VNF
Sbjct: 97426 NGSNNTIIAENVIPQNWQPGQVYRSLVNF 97512
Score = 42.8 bits (87), Expect(3) = 7e-022
Identities = 19/38 (50%), Positives = 23/38 (60%)
Frame = +3 / +2
Query: 168 GLDIAFRVDAGSNANYLAMAIEYESGDGDLRAXELKQS 281
G I F +D G+N A IEYE+G+GDL EL QS
Sbjct: 92621 GNSIVFTIDNGANPYCFATEIEYENGNGDLVQVELSQS 92734
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 547,236,527
Number of Sequences: 392609
Number of extensions: 6215190
Number of successful extensions: 34918
Number of sequences better than 1.0e-020: 13
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 22309689765
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)