TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3336981.2.1
(510 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BI309102.1|BI309102 EST530512 GPOD Medicago truncatula c... 177 6e-051
gb|AC121245.17| Medicago truncatula clone mth2-33g3, comple... 137 7e-045
gb|AC174344.6| Medicago truncatula clone mth2-167b8, WORKIN... 136 1e-044
emb|CR341804.1| mte1-74E10RM1 BAC end, cultivar Jemalong A1... 137 1e-031
emb|CR326732.1| mte1-55E9FM1 BAC end, cultivar Jemalong A17... 115 5e-025
emb|CR505658.1| mth4-39D16FM1 BAC end, cultivar Jemalong A1... 69 2e-023
emb|CR323785.1| mte1-50N19RM1 BAC end, cultivar Jemalong A1... 92 4e-023
gb|AC146331.35| Medicago truncatula clone mth2-7h18, comple... 80 5e-021
>gb|BI309102.1|BI309102 EST530512 GPOD Medicago truncatula cDNA clone pGPOD-10N9 5' end,
mRNA sequence
Length = 683
Score = 177 bits (382), Expect(2) = 6e-051
Identities = 69/141 (48%), Positives = 98/141 (69%)
Frame = +1 / +1
Query: 88 YSSIQSRCEVIMYTIALLTFMSIGGFPSFVEDVKVFRKERLSGHYGVSEFVISNTISATP 267
Y+SI +R + +TFMSIGGFPSFVED+KVF++ERL+GHYGV+ FVISNT+SATP
Sbjct: 82 YNSILARGSCASFVFGFVTFMSIGGFPSFVEDMKVFQRERLNGHYGVTAFVISNTLSATP 261
Query: 268 YLAGVSLLPGAMLYYLTGLSKGADHFVYFXXXXXXXXXXXXXXXXXIAAVVPDFLMGIIV 447
+L ++ L G + Y++ L G H+V+F IA++VP+FLMGII+
Sbjct: 262 FLILITFLSGTICYFMVNLHPGFSHYVFFVLALYASVTVVESLMMAIASIVPNFLMGIII 441
Query: 448 GAGVQGVMMLNGGFFRLPNEL 510
GAG+QG+ ML G+F+LP+++
Sbjct: 442 GAGIQGIFMLVSGYFKLPHDI 504
Score = 44.1 bits (90), Expect(2) = 6e-051
Identities = 13/25 (52%), Positives = 20/25 (80%)
Frame = +1 / +1
Query: 4 GYYWMRLAIYMGIGVCLGTIFYQLG 78
GYYW+RL IY+ + +C+GTI+ +G
Sbjct: 1 GYYWLRLVIYIVVTICIGTIYLNVG 75
>gb|AC121245.17| Medicago truncatula clone mth2-33g3, complete sequence
Length = 125763
Score = 137 bits (294), Expect(3) = 7e-045
Identities = 52/107 (48%), Positives = 75/107 (70%)
Frame = +1 / +3
Query: 190 VFRKERLSGHYGVSEFVISNTISATPYLAGVSLLPGAMLYYLTGLSKGADHFVYFXXXXX 369
VF++ER +GHYGV +VI NT S+ P++ V+++P A+ YYL GL KG +HF+YF
Sbjct: 60417 VFQRERQNGHYGVIAYVIGNTFSSIPFILLVTIIPAAITYYLPGLQKGFEHFLYFASVLF 60596
Query: 370 XXXXXXXXXXXXIAAVVPDFLMGIIVGAGVQGVMMLNGGFFRLPNEL 510
+A++VP++LMGII GAG+QG+MML GGFF+LP+E+
Sbjct: 60597 SSLMLVEGLMMIVASIVPNYLMGIITGAGIQGIMMLVGGFFKLPHEI 60737
Score = 136 bits (291), Expect(2) = 4e-036
Identities = 51/107 (47%), Positives = 75/107 (70%)
Frame = +1 / +1
Query: 190 VFRKERLSGHYGVSEFVISNTISATPYLAGVSLLPGAMLYYLTGLSKGADHFVYFXXXXX 369
VF++ER +GHYGV +VI NT S+ P++ V+++P A+ YYL GL KG +HF+YF
Sbjct: 51208 VFQRERQNGHYGVIAYVIGNTFSSIPFILLVTIIPAAITYYLPGLQKGFEHFLYFASVLF 51387
Query: 370 XXXXXXXXXXXXIAAVVPDFLMGIIVGAGVQGVMMLNGGFFRLPNEL 510
+A++VP++LMGII GAG+QG+M+L GGFF+LP+E+
Sbjct: 51388 SSLMLVESLMMIVASIVPNYLMGIITGAGIQGIMILVGGFFKLPHEI 51528
Score = 123 bits (264), Expect(2) = 2e-031
Identities = 42/107 (39%), Positives = 74/107 (69%)
Frame = +1 / +1
Query: 190 VFRKERLSGHYGVSEFVISNTISATPYLAGVSLLPGAMLYYLTGLSKGADHFVYFXXXXX 369
VF++ERL+GHYG++ F+I N +S+ PY+ +SL+ G+++ YL+GL KG +H++YF
Sbjct: 28306 VFKRERLNGHYGITAFLIGNILSSIPYMVMISLISGSIVCYLSGLHKGLEHYLYFASILF 28485
Query: 370 XXXXXXXXXXXXIAAVVPDFLMGIIVGAGVQGVMMLNGGFFRLPNEL 510
+ ++ P+F+MG+I+ GV+G+ +L GGF+R+P++L
Sbjct: 28486 AIMMWVESLMMVVGSIFPNFVMGVIIAGGVEGLAILTGGFYRIPSDL 28626
Score = 91.8 bits (194), Expect(4) = 3e-043
Identities = 34/55 (61%), Positives = 44/55 (80%)
Frame = +1 / -1
Query: 190 VFRKERLSGHYGVSEFVISNTISATPYLAGVSLLPGAMLYYLTGLSKGADHFVYF 354
VF +ERL+GHYGV+ +VI NT+SA PYL +SL+PGA+ YY GL KG +HF+YF
Sbjct: 73254 VFERERLNGHYGVTAYVIGNTLSAIPYLLMISLIPGAIAYYPPGLQKGFEHFIYF 73090
Score = 77.1 bits (162), Expect(2) = 3e-022
Identities = 27/56 (48%), Positives = 41/56 (73%)
Frame = +1 / +3
Query: 187 KVFRKERLSGHYGVSEFVISNTISATPYLAGVSLLPGAMLYYLTGLSKGADHFVYF 354
+VF +ERL+GHYGV+ F+I N S+ PY+ +S++PG + Y L + KG +HF+YF
Sbjct: 20742 QVFERERLNGHYGVTAFLIGNIFSSLPYIIMISVIPGGIAYQLCKMHKGLEHFLYF 20909
Score = 73.5 bits (154), Expect(4) = 3e-043
Identities = 27/35 (77%), Positives = 33/35 (94%)
Frame = +1 / -1
Query: 406 IAAVVPDFLMGIIVGAGVQGVMMLNGGFFRLPNEL 510
+A++VPDFLMGII GAG+QG+MML GGFFRLPN+L
Sbjct: 73038 VASIVPDFLMGIITGAGIQGIMMLAGGFFRLPNDL 72934
Score = 51.5 bits (106), Expect(3) = 7e-045
Identities = 21/38 (55%), Positives = 27/38 (71%)
Frame = +1 / +2
Query: 88 YSSIQSRCEVIMYTIALLTFMSIGGFPSFVEDVKVFRK 201
+S Q+R ++ + LTFMSIGGFPSFVED+KV K
Sbjct: 50699 FSLFQNRGSLLSFIAGFLTFMSIGGFPSFVEDMKVVPK 50812
Score = 50.1 bits (103), Expect(4) = 3e-043
Identities = 19/34 (55%), Positives = 26/34 (76%)
Frame = +1 / -3
Query: 91 SSIQSRCEVIMYTIALLTFMSIGGFPSFVEDVKV 192
S Q R ++M+ + LTFM+IGGFPS+VED+KV
Sbjct: 73666 SLFQDRGSLLMFVFSFLTFMTIGGFPSYVEDMKV 73565
Score = 48.3 bits (99), Expect(2) = 3e-022
Identities = 15/35 (42%), Positives = 28/35 (80%)
Frame = +1 / +2
Query: 406 IAAVVPDFLMGIIVGAGVQGVMMLNGGFFRLPNEL 510
+ A +P++++G+ + G++G+M+L GF+RLPNEL
Sbjct: 20963 LEASLPNYVIGMFITGGIEGLMILTAGFYRLPNEL 21067
Score = 47.3 bits (97), Expect(3) = 7e-045
Identities = 15/25 (60%), Positives = 20/25 (80%)
Frame = +1 / +2
Query: 4 GYYWMRLAIYMGIGVCLGTIFYQLG 78
GYYW+RL IY+ + + LGTI+Y LG
Sbjct: 50510 GYYWLRLVIYVALAISLGTIYYDLG 50584
Score = 45.1 bits (92), Expect(2) = 4e-036
Identities = 15/31 (48%), Positives = 23/31 (74%)
Frame = +1 / +1
Query: 1 VGYYWMRLAIYMGIGVCLGTIFYQLGYSTYS 93
+ YW+RL +++ I + LG+IFYQ+G ST S
Sbjct: 27445 ISNYWLRLVVFVAIAISLGSIFYQVGSSTRS 27537
Score = 41.4 bits (84), Expect(2) = 2e-031
Identities = 14/32 (43%), Positives = 24/32 (75%)
Frame = +1 / +3
Query: 1 VGYYWMRLAIYMGIGVCLGTIFYQLGYSTYSS 96
VG YW+RL +++ I + +G+IFY +G S+ S+
Sbjct: 19860 VGNYWLRLVVFVMIAISIGSIFYGIGSSSGSA 19955
Score = 40.0 bits (81), Expect(4) = 3e-043
Identities = 14/31 (45%), Positives = 20/31 (64%)
Frame = +1 / -1
Query: 1 VGYYWMRLAIYMGIGVCLGTIFYQLGYSTYS 93
+GYYW+RL IY+ + + L T+F L S S
Sbjct: 73833 LGYYWLRLGIYIALAISLATVFNDLDKSNGS 73741
>gb|AC174344.6| Medicago truncatula clone mth2-167b8, WORKING DRAFT SEQUENCE, 24
unordered pieces
Length = 100955
Score = 136 bits (291), Expect(3) = 1e-044
Identities = 51/107 (47%), Positives = 75/107 (70%)
Frame = +1 / -1
Query: 190 VFRKERLSGHYGVSEFVISNTISATPYLAGVSLLPGAMLYYLTGLSKGADHFVYFXXXXX 369
VF++ER +GHYGV +VI NT S+ P++ V+++P A+ YYL GL KG +HF+YF
Sbjct: 64538 VFQRERQNGHYGVIAYVIGNTFSSIPFILLVTIIPAAITYYLPGLQKGFEHFLYFASVLF 64359
Query: 370 XXXXXXXXXXXXIAAVVPDFLMGIIVGAGVQGVMMLNGGFFRLPNEL 510
+A++VP++LMGII GAG+QG+M+L GGFF+LP+E+
Sbjct: 64358 SSLMLVESLMMIVASIVPNYLMGIITGAGIQGIMILVGGFFKLPHEI 64218
Score = 123 bits (263), Expect(2) = 2e-032
Identities = 42/107 (39%), Positives = 73/107 (68%)
Frame = +1 / +3
Query: 190 VFRKERLSGHYGVSEFVISNTISATPYLAGVSLLPGAMLYYLTGLSKGADHFVYFXXXXX 369
VF++ERL+GHYG++ F+I N +S+ PY+ +SL+ G+++ YL+GL KG +H++YF
Sbjct: 9198 VFKRERLNGHYGITAFLIGNILSSIPYMVMISLISGSIVCYLSGLHKGLEHYLYFASILF 9377
Query: 370 XXXXXXXXXXXXIAAVVPDFLMGIIVGAGVQGVMMLNGGFFRLPNEL 510
+ ++ P+F+MG+I+ GV+G+ +L GGF+R+P +L
Sbjct: 9378 AIMMWVESLMMVVGSIFPNFVMGVIIAGGVEGLAILTGGFYRIPTDL 9518
Score = 107 bits (229), Expect(2) = 4e-029
Identities = 40/108 (37%), Positives = 67/108 (62%)
Frame = +1 / -2
Query: 187 KVFRKERLSGHYGVSEFVISNTISATPYLAGVSLLPGAMLYYLTGLSKGADHFVYFXXXX 366
+VF +ERL+GHYG + F+I N S+ PY+ +S+LP + Y L + KG +HF+YF
Sbjct: 37477 QVFERERLNGHYGDTAFLIGNIFSSLPYIIMISVLPV*VAYQLCKMHKGLEHFLYFISLL 37298
Query: 367 XXXXXXXXXXXXXIAAVVPDFLMGIIVGAGVQGVMMLNGGFFRLPNEL 510
+ ++ P++++G+ + G++G+M+L GF+RLPNEL
Sbjct: 37297 IAIVMWVESLMLVVGSISPNYVIGMFITGGIEGLMILTAGFYRLPNEL 37154
Score = 66.1 bits (138), Expect(5) = 2e-036
Identities = 23/35 (65%), Positives = 33/35 (94%)
Frame = +1 / -2
Query: 406 IAAVVPDFLMGIIVGAGVQGVMMLNGGFFRLPNEL 510
+A++VP++LMGII GAG+QG+MML GGFF+LP+E+
Sbjct: 17764 VASIVPNYLMGIITGAGIQGIMMLVGGFFKLPHEI 17660
Score = 64.3 bits (134), Expect(5) = 2e-036
Identities = 24/38 (63%), Positives = 32/38 (84%)
Frame = +1 / -3
Query: 190 VFRKERLSGHYGVSEFVISNTISATPYLAGVSLLPGAM 303
VF +ERL+GHYGV+ +VI NT+SA PYL +SL+PGA+
Sbjct: 51069 VFERERLNGHYGVTAYVIGNTLSAIPYLLMISLIPGAI 50956
Score = 51.5 bits (106), Expect(3) = 1e-044
Identities = 21/38 (55%), Positives = 27/38 (71%)
Frame = +1 / -2
Query: 88 YSSIQSRCEVIMYTIALLTFMSIGGFPSFVEDVKVFRK 201
+S Q+R ++ + LTFMSIGGFPSFVED+KV K
Sbjct: 65047 FSLFQNRGSLLSFIAGFLTFMSIGGFPSFVEDMKVVPK 64934
Score = 50.1 bits (103), Expect(5) = 2e-036
Identities = 19/34 (55%), Positives = 26/34 (76%)
Frame = +1 / -2
Query: 91 SSIQSRCEVIMYTIALLTFMSIGGFPSFVEDVKV 192
S Q R ++M+ + LTFM+IGGFPS+VED+KV
Sbjct: 51481 SLFQDRGSLLMFVFSFLTFMTIGGFPSYVEDMKV 51380
Score = 49.6 bits (102), Expect(2) = 4e-029
Identities = 19/37 (51%), Positives = 25/37 (67%)
Frame = +1 / -1
Query: 91 SSIQSRCEVIMYTIALLTFMSIGGFPSFVEDVKVFRK 201
S Q R ++ + +TFMSIGGFPSFVED+K+ K
Sbjct: 56024 SLFQDRGSLLSFIFGFITFMSIGGFPSFVEDMKIITK 55914
Score = 47.3 bits (97), Expect(3) = 1e-044
Identities = 15/25 (60%), Positives = 20/25 (80%)
Frame = +1 / -2
Query: 4 GYYWMRLAIYMGIGVCLGTIFYQLG 78
GYYW+RL IY+ + + LGTI+Y LG
Sbjct: 65236 GYYWLRLVIYVALAISLGTIYYDLG 65162
Score = 45.1 bits (92), Expect(2) = 2e-032
Identities = 15/31 (48%), Positives = 23/31 (74%)
Frame = +1 / +3
Query: 1 VGYYWMRLAIYMGIGVCLGTIFYQLGYSTYS 93
+ YW+RL +++ I + LG+IFYQ+G ST S
Sbjct: 8337 ISNYWLRLVVFVAIAISLGSIFYQVGSSTRS 8429
Score = 40.0 bits (81), Expect(5) = 2e-036
Identities = 14/31 (45%), Positives = 20/31 (64%)
Frame = +1 / -3
Query: 1 VGYYWMRLAIYMGIGVCLGTIFYQLGYSTYS 93
+GYYW+RL IY+ + + L T+F L S S
Sbjct: 51648 LGYYWLRLGIYIALAISLATVFNDLDKSNGS 51556
Score = 34.1 bits (68), Expect(5) = 2e-036
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = +1 / -1
Query: 301 MLYYLTGLSKGADHFVYF 354
+++YL GL KG DHF YF
Sbjct: 17870 LIFYLPGLQKGFDHFFYF 17817
>emb|CR341804.1| mte1-74E10RM1 BAC end, cultivar Jemalong A17 of Medicago
truncatula, genomic survey sequence
Length = 525
Score = 137 bits (294), Expect = 1e-031
Identities = 52/107 (48%), Positives = 75/107 (70%)
Frame = +1 / +1
Query: 190 VFRKERLSGHYGVSEFVISNTISATPYLAGVSLLPGAMLYYLTGLSKGADHFVYFXXXXX 369
VF++ER +GHYGV +VI NT S+ P++ V+++P A+ YYL GL KG +HF+YF
Sbjct: 22 VFQRERQNGHYGVIAYVIGNTFSSIPFILLVTIIPAAITYYLPGLQKGFEHFLYFASVLF 201
Query: 370 XXXXXXXXXXXXIAAVVPDFLMGIIVGAGVQGVMMLNGGFFRLPNEL 510
+A++VP++LMGII GAG+QG+MML GGFF+LP+E+
Sbjct: 202 SSLMLVEGLMMIVASIVPNYLMGIITGAGIQGIMMLVGGFFKLPHEI 342
>emb|CR326732.1| mte1-55E9FM1 BAC end, cultivar Jemalong A17 of Medicago truncatula,
genomic survey sequence
Length = 684
Score = 115 bits (246), Expect = 5e-025
Identities = 41/107 (38%), Positives = 68/107 (63%)
Frame = +1 / -3
Query: 190 VFRKERLSGHYGVSEFVISNTISATPYLAGVSLLPGAMLYYLTGLSKGADHFVYFXXXXX 369
VF +ERL+GHYGV+ F+I N S+ PY+ +S++PG + Y L + KG +HF+YF
Sbjct: 682 VFERERLNGHYGVTAFLIGNIFSSLPYIIMISVIPGGIAYQLCKMHKGLEHFLYFISLLI 503
Query: 370 XXXXXXXXXXXXIAAVVPDFLMGIIVGAGVQGVMMLNGGFFRLPNEL 510
+ ++ P++++G+ + G++G+M+L GF+RLPNEL
Sbjct: 502 AIVMWVESLMLVVGSISPNYVIGMFITGGIEGLMILTAGFYRLPNEL 362
>emb|CR505658.1| mth4-39D16FM1 BAC end, cultivar Jemalong A17 of Medicago
truncatula, genomic survey sequence
Length = 537
Score = 69.3 bits (145), Expect(2) = 2e-023
Identities = 25/35 (71%), Positives = 32/35 (91%)
Frame = +1 / -2
Query: 406 IAAVVPDFLMGIIVGAGVQGVMMLNGGFFRLPNEL 510
+A++VPDF+MG I GAG+QG+MML GGFFRLPN+L
Sbjct: 374 VASIVPDFIMGNITGAGIQGIMMLAGGFFRLPNDL 270
Score = 60.2 bits (125), Expect(2) = 2e-023
Identities = 22/36 (61%), Positives = 28/36 (77%)
Frame = +1 / -2
Query: 247 NTISATPYLAGVSLLPGAMLYYLTGLSKGADHFVYF 354
NT+SA PYL +SL+PGA+ YY GL KG +HF+YF
Sbjct: 533 NTLSAIPYLLMISLIPGAIAYYPPGLQKGFEHFIYF 426
>emb|CR323785.1| mte1-50N19RM1 BAC end, cultivar Jemalong A17 of Medicago
truncatula, genomic survey sequence
Length = 568
Score = 91.8 bits (194), Expect(2) = 4e-023
Identities = 34/55 (61%), Positives = 44/55 (80%)
Frame = +1 / -2
Query: 190 VFRKERLSGHYGVSEFVISNTISATPYLAGVSLLPGAMLYYLTGLSKGADHFVYF 354
VF +ERL+GHYGV+ +VI NT+SA PYL +SL+PGA+ YY GL KG +HF+YF
Sbjct: 270 VFERERLNGHYGVTAYVIGNTLSAIPYLLMISLIPGAIAYYPPGLQKGFEHFIYF 106
Score = 36.8 bits (74), Expect(2) = 4e-023
Identities = 12/18 (66%), Positives = 17/18 (94%)
Frame = +1 / -2
Query: 406 IAAVVPDFLMGIIVGAGV 459
+A++VPDF+MGII GAG+
Sbjct: 54 VASIVPDFIMGIITGAGI 1
>gb|AC146331.35| Medicago truncatula clone mth2-7h18, complete sequence
Length = 138838
Score = 79.9 bits (168), Expect(3) = 5e-021
Identities = 31/96 (32%), Positives = 56/96 (58%)
Frame = +1 / +1
Query: 172 FVEDVKVFRKERLSGHYGVSEFVISNTISATPYLAGVSLLPGAMLYYLTGLSKGADHFVY 351
F+ +VF +ER++G+YGV+ ++++N IS+ P+L ++L + Y + G H+V+
Sbjct: 106048 FIHMKQVFYRERMNGYYGVAAYILANFISSFPFLVVIALTSCTITYNMVKFRPGFIHYVF 106227
Query: 352 FXXXXXXXXXXXXXXXXXIAAVVPDFLMGIIVGAGV 459
F +A++VP+FLMGI+ GAG+
Sbjct: 106228 FTLNVYGCLSVIESIMMVVASLVPNFLMGIVTGAGI 106335
Score = 46.0 bits (94), Expect(3) = 5e-021
Identities = 12/28 (42%), Positives = 23/28 (82%)
Frame = +1 / +1
Query: 1 VGYYWMRLAIYMGIGVCLGTIFYQLGYS 84
+GYYW+R+ I + + +C+GT+++ +GYS
Sbjct: 105556 IGYYWLRIIINIIVSICVGTLYFDVGYS 105639
Score = 29.9 bits (59), Expect(3) = 5e-021
Identities = 10/18 (55%), Positives = 16/18 (88%)
Frame = +1 / +2
Query: 457 VQGVMMLNGGFFRLPNEL 510
+QG+MM++ GFFRL ++L
Sbjct: 106424 MQGIMMMSSGFFRLLSDL 106477
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 343,135,713
Number of Sequences: 392609
Number of extensions: 3303584
Number of successful extensions: 33527
Number of sequences better than 1.0e-020: 10
length of database: 147,244,331
effective HSP length: 53
effective length of database: 126,436,054
effective search space used: 14666582264
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)