TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3148257.2.1
(675 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AC150782.12| Medicago truncatula clone mth2-116a21, WORK... 188 2e-050
gb|AC158736.12| Medicago truncatula clone mth2-165n2, WORKI... 188 2e-050
emb|CR302584.1| mte1-21I1RM1 BAC end, cultivar Jemalong A17... 151 3e-039
emb|CR312700.1| mte1-35K8FM1 BAC end, cultivar Jemalong A17... 140 2e-032
gb|BQ146927.1|BQ146927 NF029F11FL1F1094 Developing flower M... 116 2e-029
gb|AL381590.1|AL381590 MtBC01H02R2 MtBC Medicago truncatula... 126 3e-028
gb|BQ123088.1|BQ123088 EST608664 GLSD Medicago truncatula c... 124 2e-027
gb|AL389186.1|AL389186 MtBC53C09R1 MtBC Medicago truncatula... 123 3e-027
gb|CX533175.1|CX533175 s13dNF0CG12MJ088_319513 Methyl Jasmo... 122 4e-027
gb|AW688473.2|AW688473 NF008A08ST1F1000 Developing stem Med... 122 8e-027
emb|CR342893.1| mte1-75J4FM1 BAC end, cultivar Jemalong A17... 96 3e-024
gb|AC158209.13| Medicago truncatula clone mth2-154h5, compl... 96 2e-022
gb|AC137603.16| Medicago truncatula clone mth2-14b10, compl... 99 2e-021
>gb|AC150782.12| Medicago truncatula clone mth2-116a21, WORKING DRAFT SEQUENCE, 3
unordered pieces
Length = 118726
Score = 188 bits (406), Expect(2) = 2e-050
Identities = 71/102 (69%), Positives = 91/102 (89%)
Frame = +3 / +3
Query: 111 KFTEFQIIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIM 290
+FTEF+ +DIFTSLLAF+PTGWGL+ IAQV RPF++ST++W +++VARLY+IL GV IM
Sbjct: 21972 EFTEFKFVDIFTSLLAFLPTGWGLLLIAQVFRPFLQSTIIWSGVVAVARLYDILFGVIIM 22151
Query: 291 APVALLSWLPGFQEMQTRVLFNEGFSRGLQISRILTGKRTNT 416
PVALLSWLPGFQ MQTR+LFNE FSRGL+IS+I+TGK++ +
Sbjct: 22152 TPVALLSWLPGFQNMQTRILFNEAFSRGLRISQIVTGKKSQS 22277
Score = 128 bits (274), Expect = 9e-029
Identities = 54/127 (42%), Positives = 78/127 (61%)
Frame = -2 / -3
Query: 401 PSKNTGYLEAPAKTFIEKYPCLHFLKSRQPGQQCNWCHNEHSKQYLIQTSHRNNTVPDHS 222
P N GY EAPA+ F+EK LH LK+RQP C+ HN SK Y+I+TSH NNT P++
Sbjct: 22262 PCNNLGYAEAPAECFVEKNSSLHILKARQP***CHRSHNNDSK*YIIKTSHCNNTTPNYR 22083
Query: 221 GFDEWPNYLSDRNQAPTSRNKCQKTSEDVNDLKFRELQKQNQH*HYKSDDSSLNNPVVKV 42
EW L ++ QAP+ +CQ+ S+++++ +F E Q+ N + K+ D LN PV+ +
Sbjct: 22082 RLQEWSENLGNQ*QAPSCGKECQQASKNIHEFEFCEFQQSNYQKYCKNYDKELNKPVIDM 21903
Query: 41 LLCGILV 21
L I V
Sbjct: 21902 LFRRIFV 21882
Score = 116 bits (247), Expect = 5e-025
Identities = 57/97 (58%), Positives = 65/97 (67%)
Frame = -3 / -1
Query: 403 FPVRIRDIWRPLLKPSLKSTLVCIS*NPGNQDSNATGAIMNTPSSISYKRATEIILSQTT 224
FPV I D+ RPLL SLK LVCI * PGN DSNATG I+ TP++IS +RAT Q
Sbjct: 22264 FPVTIWDMRRPLLNASLKRILVCIF*KPGNHDSNATGVIIMTPNNIS*RRATATTPLQII 22085
Query: 223 VDSMNGRIT*AIEIKPQPVGINAKRLVKMSMI*NSVN 113
VD NGR T AI +P PVG NA +LVK+S NSVN
Sbjct: 22084 VDCKNGRKTWAINSRPHPVGRNANKLVKISTNLNSVN 21974
Score = 31.8 bits (63), Expect(2) = 2e-050
Identities = 10/14 (71%), Positives = 14/14 (100%)
Frame = +3 / +3
Query: 15 ARDKYAAKQHLYYR 56
AR+KY+AK+H+YYR
Sbjct: 21876 ARNKYSAKEHIYYR 21917
>gb|AC158736.12| Medicago truncatula clone mth2-165n2, WORKING DRAFT SEQUENCE
Length = 118519
Score = 188 bits (406), Expect(2) = 2e-050
Identities = 71/102 (69%), Positives = 91/102 (89%)
Frame = +3 / -1
Query: 111 KFTEFQIIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIM 290
+FTEF+ +DIFTSLLAF+PTGWGL+ IAQV RPF++ST++W +++VARLY+IL GV IM
Sbjct: 10825 EFTEFKFVDIFTSLLAFLPTGWGLLLIAQVFRPFLQSTIIWSGVVAVARLYDILFGVIIM 10646
Query: 291 APVALLSWLPGFQEMQTRVLFNEGFSRGLQISRILTGKRTNT 416
PVALLSWLPGFQ MQTR+LFNE FSRGL+IS+I+TGK++ +
Sbjct: 10645 TPVALLSWLPGFQNMQTRILFNEAFSRGLRISQIVTGKKSQS 10520
Score = 128 bits (274), Expect = 9e-029
Identities = 54/127 (42%), Positives = 78/127 (61%)
Frame = -2 / +2
Query: 401 PSKNTGYLEAPAKTFIEKYPCLHFLKSRQPGQQCNWCHNEHSKQYLIQTSHRNNTVPDHS 222
P N GY EAPA+ F+EK LH LK+RQP C+ HN SK Y+I+TSH NNT P++
Sbjct: 10535 PCNNLGYAEAPAECFVEKNSSLHILKARQP***CHRSHNNDSK*YIIKTSHCNNTTPNYR 10714
Query: 221 GFDEWPNYLSDRNQAPTSRNKCQKTSEDVNDLKFRELQKQNQH*HYKSDDSSLNNPVVKV 42
EW L ++ QAP+ +CQ+ S+++++ +F E Q+ N + K+ D LN PV+ +
Sbjct: 10715 RLQEWSENLGNQ*QAPSCGKECQQASKNIHEFEFCEFQQSNYQKYCKNYDKELNKPVIDM 10894
Query: 41 LLCGILV 21
L I V
Sbjct: 10895 LFRRIFV 10915
Score = 116 bits (247), Expect = 5e-025
Identities = 57/97 (58%), Positives = 65/97 (67%)
Frame = -3 / +3
Query: 403 FPVRIRDIWRPLLKPSLKSTLVCIS*NPGNQDSNATGAIMNTPSSISYKRATEIILSQTT 224
FPV I D+ RPLL SLK LVCI * PGN DSNATG I+ TP++IS +RAT Q
Sbjct: 10533 FPVTIWDMRRPLLNASLKRILVCIF*KPGNHDSNATGVIIMTPNNIS*RRATATTPLQII 10712
Query: 223 VDSMNGRIT*AIEIKPQPVGINAKRLVKMSMI*NSVN 113
VD NGR T AI +P PVG NA +LVK+S NSVN
Sbjct: 10713 VDCKNGRKTWAINSRPHPVGRNANKLVKISTNLNSVN 10823
Score = 31.8 bits (63), Expect(2) = 2e-050
Identities = 10/14 (71%), Positives = 14/14 (100%)
Frame = +3 / -1
Query: 15 ARDKYAAKQHLYYR 56
AR+KY+AK+H+YYR
Sbjct: 10921 ARNKYSAKEHIYYR 10880
>emb|CR302584.1| mte1-21I1RM1 BAC end, cultivar Jemalong A17 of Medicago truncatula,
genomic survey sequence
Length = 651
Score = 151 bits (325), Expect(2) = 3e-039
Identities = 56/79 (70%), Positives = 70/79 (88%)
Frame = +3 / -1
Query: 111 KFTEFQIIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIM 290
+FTEF+ +DIFTSLLAF+PTGWGL+ IAQV RPF++ST++W +++VARLY+IL GV IM
Sbjct: 237 EFTEFKFVDIFTSLLAFLPTGWGLLLIAQVFRPFLQSTIIWSGVVAVARLYDILFGVIIM 58
Query: 291 APVALLSWLPGFQEMQTRV 347
PVALLSWLPGFQ MQTR+
Sbjct: 57 TPVALLSWLPGFQNMQTRI 1
Score = 101 bits (216), Expect = 9e-021
Identities = 43/106 (40%), Positives = 65/106 (61%)
Frame = -2 / +1
Query: 338 LHFLKSRQPGQQCNWCHNEHSKQYLIQTSHRNNTVPDHSGFDEWPNYLSDRNQAPTSRNK 159
LH LK+RQP C+ HN SK Y+I+TSH NNT P++ EW L ++ QAP+ +
Sbjct: 10 LHILKARQP***CHRSHNNDSK*YIIKTSHCNNTTPNYRRLQEWSENLGNQ*QAPSCGKE 189
Query: 158 CQKTSEDVNDLKFRELQKQNQH*HYKSDDSSLNNPVVKVLLCGILV 21
CQ+ S+++++ +F E Q+ N + K+ D LN PV+ +L I V
Sbjct: 190 CQQASKNIHEFEFCEFQQSNYQKYCKNYDKELNKPVIDMLFRRIFV 327
Score = 31.8 bits (63), Expect(2) = 3e-039
Identities = 10/14 (71%), Positives = 14/14 (100%)
Frame = +3 / -1
Query: 15 ARDKYAAKQHLYYR 56
AR+KY+AK+H+YYR
Sbjct: 333 ARNKYSAKEHIYYR 292
>emb|CR312700.1| mte1-35K8FM1 BAC end, cultivar Jemalong A17 of Medicago truncatula,
genomic survey sequence
Length = 377
Score = 140 bits (300), Expect = 2e-032
Identities = 54/82 (65%), Positives = 69/82 (84%)
Frame = +3 / +1
Query: 171 GWGLISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIMAPVALLSWLPGFQEMQTRVL 350
GWGL+ IAQV F+ ST++W +++VARLY+IL GV IM PVALLSWLPGFQ MQTR+L
Sbjct: 7 GWGLLLIAQVFLLFLLSTIIWSGVVAVARLYDILFGVIIMTPVALLSWLPGFQNMQTRIL 186
Query: 351 FNEGFSRGLQISRILTGKRTNT 416
FNE FSRGL+IS+I+TGK++ +
Sbjct: 187 FNEAFSRGLRISQIVTGKKSQS 252
>gb|BQ146927.1|BQ146927 NF029F11FL1F1094 Developing flower Medicago truncatula cDNA clone
NF029F11FL 5', mRNA sequence
Length = 694
Score = 116 bits (249), Expect(3) = 2e-029
Identities = 44/67 (65%), Positives = 56/67 (83%)
Frame = +3 / +3
Query: 111 KFTEFQIIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIM 290
+FTEF+ +DIFTSLLAF+PTGWGL+ IAQV RPF++ST++W + + ARLY+IL GV IM
Sbjct: 381 EFTEFKFVDIFTSLLAFLPTGWGLLLIAQVFRPFLQSTIIWSGVXAXARLYDILFGVIIM 560
Query: 291 APVALLS 311
P ALLS
Sbjct: 561 TPXALLS 581
Score = 31.8 bits (63), Expect(3) = 2e-029
Identities = 10/14 (71%), Positives = 14/14 (100%)
Frame = +3 / +3
Query: 15 ARDKYAAKQHLYYR 56
AR+KY+AK+H+YYR
Sbjct: 285 ARNKYSAKEHIYYR 326
Score = 21.7 bits (41), Expect(3) = 2e-029
Identities = 8/17 (47%), Positives = 10/17 (58%)
Frame = +3 / +2
Query: 324 FQEMQTRVLFNEGFSRG 374
F+ Q +LFN F RG
Sbjct: 596 FKICQPXILFNXAFXRG 646
>gb|AL381590.1|AL381590 MtBC01H02R2 MtBC Medicago truncatula cDNA clone MtBC01H02 T7, mRNA
sequence
Length = 548
Score = 126 bits (270), Expect = 3e-028
Identities = 48/91 (52%), Positives = 66/91 (72%)
Frame = +3 / +2
Query: 135 DIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIMAPVALLSW 314
DI +LAF+PTGWG++ IAQ ++P + W S+ ++AR YEI++G+ + PVA L+W
Sbjct: 95 DIIVCILAFMPTGWGMLQIAQALKPIVRRAGFWGSVKTLARGYEIVMGLLLFTPVAFLAW 274
Query: 315 LPGFQEMQTRVLFNEGFSRGLQISRILTGKR 407
P E QTR+LFN+ FSRGLQISRIL G+R
Sbjct: 275 FPFVSEFQTRMLFNQAFSRGLQISRILGGQR 367
>gb|BQ123088.1|BQ123088 EST608664 GLSD Medicago truncatula cDNA clone pGLSD-31G15, mRNA
sequence
Length = 673
Score = 124 bits (265), Expect = 2e-027
Identities = 48/93 (51%), Positives = 67/93 (72%)
Frame = +3 / +2
Query: 129 IIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIMAPVALL 308
I DI +LA +PTGWG++ IAQ +P I T +W S+ ++AR YE+++G+ + PVA L
Sbjct: 239 IKDIIICILAVMPTGWGMLQIAQACKPLIAKTGLWGSVRALARGYEVIMGLLLFTPVAFL 418
Query: 309 SWLPGFQEMQTRVLFNEGFSRGLQISRILTGKR 407
+W P E QTR+LFN+ FSRGLQISRIL G++
Sbjct: 419 AWFPFVSEFQTRMLFNQAFSRGLQISRILGGQK 517
>gb|AL389186.1|AL389186 MtBC53C09R1 MtBC Medicago truncatula cDNA clone MtBC53C09 T7, mRNA
sequence
Length = 544
Score = 123 bits (263), Expect = 3e-027
Identities = 48/100 (48%), Positives = 67/100 (67%)
Frame = +3 / +1
Query: 117 TEFQIIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIMAP 296
T+ + D+F S+LAFIPTGWG++SIA +P ++ +W I S+ARLY+ +G+ I P
Sbjct: 73 TDLSVPDVFASILAFIPTGWGILSIAAAWKPVMKRLGLWKFIRSLARLYDAGMGMLIFVP 252
Query: 297 VALLSWLPGFQEMQTRVLFNEGFSRGLQISRILTGKRTNT 416
+A SW P QTR++FN+ FSRGL+IS IL G NT
Sbjct: 253 IAFFSWFPFVSTFQTRLMFNQAFSRGLEISLILAGNNPNT 372
>gb|CX533175.1|CX533175 s13dNF0CG12MJ088_319513 Methyl Jasmonate-Elicited Root Cell
Suspension Culture Medicago truncatula cDNA, mRNA
sequence
Length = 611
Score = 122 bits (262), Expect = 4e-027
Identities = 47/100 (47%), Positives = 70/100 (70%)
Frame = +3 / +2
Query: 114 FTEFQIIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIMA 293
FT I D+F S+LAFIPTGWG++S+A + ++S +W+S+ AR+Y+ +G+ I +
Sbjct: 41 FTPLSIPDLFASILAFIPTGWGILSLAITWKGIMKSLGLWESVREFARMYDAGMGMIIFS 220
Query: 294 PVALLSWLPGFQEMQTRVLFNEGFSRGLQISRILTGKRTN 413
P+A LSW P Q+R+LFN+ FSRGL+IS IL+G + N
Sbjct: 221 PIAFLSWFPFISTFQSRLLFNQAFSRGLEISLILSGNKAN 340
>gb|AW688473.2|AW688473 NF008A08ST1F1000 Developing stem Medicago truncatula cDNA clone
NF008A08ST 5', mRNA sequence
Length = 788
Score = 122 bits (260), Expect = 8e-027
Identities = 46/93 (49%), Positives = 66/93 (70%)
Frame = +3 / +2
Query: 129 IIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIMAPVALL 308
I DI +LA +PTGWG++ IAQ +P I T +W S+ +AR YE+++G+ + P+A L
Sbjct: 398 IKDIIICILAVMPTGWGMLQIAQACKPLIAKTGLWGSVRXLARGYEVIMGLLLFTPIAFL 577
Query: 309 SWLPGFQEMQTRVLFNEGFSRGLQISRILTGKR 407
+W P E QTR+LFN+ FS+GLQISRIL G++
Sbjct: 578 AWFPFVSEFQTRMLFNQAFSKGLQISRILGGQK 676
>emb|CR342893.1| mte1-75J4FM1 BAC end, cultivar Jemalong A17 of Medicago truncatula,
genomic survey sequence
Length = 724
Score = 95.9 bits (203), Expect(2) = 3e-024
Identities = 38/75 (50%), Positives = 53/75 (70%)
Frame = +3 / -1
Query: 189 IAQVIRPFIESTVVWDSIISVARLYEILLGVFIMAPVALLSWLPGFQEMQTRVLFNEGFS 368
IAQ RP +++ +W S+ +++R YE L+GV I PVA+L+W P E QTR+L+N+ FS
Sbjct: 376 IAQACRPVVKAIGMWGSVKALSRGYEYLMGVVIFTPVAILAWFPFVSEFQTRLLYNQAFS 197
Query: 369 RGLQISRILTGKRTN 413
RGLQI RIL G + N
Sbjct: 196 RGLQIQRILAGGKKN 152
Score = 39.1 bits (79), Expect(2) = 3e-024
Identities = 13/19 (68%), Positives = 16/19 (84%)
Frame = +3 / -1
Query: 135 DIFTSLLAFIPTGWGLISI 191
DIF SLLAF+PTGW L+ +
Sbjct: 718 DIFASLLAFLPTGWALVQV 662
>gb|AC158209.13| Medicago truncatula clone mth2-154h5, complete sequence
Length = 93864
Score = 95.9 bits (203), Expect(2) = 2e-022
Identities = 38/75 (50%), Positives = 53/75 (70%)
Frame = +3 / -3
Query: 189 IAQVIRPFIESTVVWDSIISVARLYEILLGVFIMAPVALLSWLPGFQEMQTRVLFNEGFS 368
IAQ RP +++ +W S+ +++R YE L+GV I PVA+L+W P E QTR+L+N+ FS
Sbjct: 54328 IAQACRPVVKAIGMWGSVKALSRGYEYLMGVVIFTPVAILAWFPFVSEFQTRLLYNQAFS 54149
Query: 369 RGLQISRILTGKRTN 413
RGLQI RIL G + N
Sbjct: 54148 RGLQIQRILAGGKKN 54104
Score = 39.6 bits (80), Expect(2) = 2e-022
Identities = 14/23 (60%), Positives = 17/23 (73%)
Frame = +3 / -3
Query: 123 FQIIDIFTSLLAFIPTGWGLISI 191
F DIF SLLAF+PTGW L+ +
Sbjct: 54682 FTFGDIFASLLAFLPTGWALVQV 54614
>gb|AC137603.16| Medicago truncatula clone mth2-14b10, complete sequence
Length = 109793
Score = 98.7 bits (209), Expect(2) = 2e-021
Identities = 37/76 (48%), Positives = 55/76 (72%)
Frame = +3 / -3
Query: 180 LISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIMAPVALLSWLPGFQEMQTRVLFNE 359
++ IAQ ++P + W+S+ ++AR YE+++G+ + PVA L+W P E QTR+LFN+
Sbjct: 60207 IMQIAQALKPLVRRGGFWESVKTLARGYEVIMGLLLFTPVAFLAWFPFVSEFQTRMLFNQ 60028
Query: 360 GFSRGLQISRILTGKR 407
FSRGLQISRIL G+R
Sbjct: 60027 AFSRGLQISRILGGQR 59980
Score = 33.6 bits (67), Expect(2) = 2e-021
Identities = 10/19 (52%), Positives = 15/19 (78%)
Frame = +3 / -2
Query: 135 DIFTSLLAFIPTGWGLISI 191
DI +LAF+PTGWG++ +
Sbjct: 60505 DIVVCILAFMPTGWGMLQV 60449
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 656,873,784
Number of Sequences: 392609
Number of extensions: 9130641
Number of successful extensions: 46097
Number of sequences better than 1.0e-020: 17
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 21427385650
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)