TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3115474.2.1
(798 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AC147538.15| Medicago truncatula clone mth2-135c7, WORKI... 167 2e-061
gb|AC147538.16| Medicago truncatula clone mth2-135c7, WORKI... 167 2e-061
gb|AC170800.5| Medicago truncatula clone mth2-32n7, complet... 124 3e-043
gb|AC141922.19| Medicago truncatula clone mth2-9n11, comple... 124 4e-043
gb|AC144931.28| Medicago truncatula clone mth2-22b18, compl... 124 5e-043
gb|AC142094.12| Medicago truncatula clone mth2-34h6, comple... 124 1e-042
gb|CX520904.1|CX520904 s13dNF58E12VI099_449594 Virus-Infect... 155 2e-042
gb|AC174287.2| Medicago truncatula clone mth2-32a8, WORKING... 123 4e-039
emb|CR955006.2| Medicago truncatula chromosome 5 clone mth2... 123 5e-039
gb|AC160840.20| Medicago truncatula clone mth2-27o21, WORKI... 123 5e-039
emb|CT571263.1| Medicago truncatula chromosome 5 clone mte1... 123 6e-039
gb|CX527617.1|CX527617 s13dNF45E07AT055_515746 Aphid-Infect... 133 4e-034
gb|CX532999.1|CX532999 s13dNF89B04MJ029_272276 Methyl Jasmo... 107 3e-033
gb|AC146785.12| Medicago truncatula clone mth2-4h4, complet... 122 6e-031
emb|CR932957.2| Medicago truncatula chromosome 5 clone mth2... 122 6e-031
gb|CX531807.1|CX531807 s13dNF87G02MJ008_257556 Methyl Jasmo... 78 2e-024
>gb|AC147538.15| Medicago truncatula clone mth2-135c7, WORKING DRAFT SEQUENCE, 5 ordered
pieces
Length = 133186
Score = 167 bits (360), Expect(3) = 2e-061
Identities = 76/129 (58%), Positives = 92/129 (71%)
Frame = -3 / -2
Query: 595 KLFLLENPKSSVKSNSHIFREYNLNVIMKPLRASMLQVSLQRALGGIDKLHCRNGVVGNS 416
KLF+L N SS ++ S +N V+ KPLRASML SLQRA+G +K + RNG
Sbjct: 59787 KLFILVNSSSSFRAGSTASCLHNPTVVTKPLRASMLAASLQRAMGVGNKGNPRNGEHQGL 59608
Query: 415 TLGSLLHKKRIIVVDDNIVNLKVAAGALKKYGAEVTCADSGKEAITLLKPPHNFDACFMD 236
+L LL ++I++VDDN VN VAAGALKKYGA V C SG EAIT+L+PPH FDACFMD
Sbjct: 59607 SLKHLLSGRKILIVDDNSVNRTVAAGALKKYGAGVVCVSSGIEAITMLRPPHQFDACFMD 59428
Query: 235 IQMPEMDGF 209
IQMPEMDG+
Sbjct: 59427 IQMPEMDGY 59401
Score = 106 bits (226), Expect(3) = 2e-030
Identities = 57/106 (53%), Positives = 66/106 (62%)
Frame = +3 / +2
Query: 213 PSISGICMSMKQASKL*GGFSSVIASFPLSAQVTSAPYFFRAPAATLRFTILSSTTMIRF 392
PSISGI +SMK AS GG S V+AS PL Q T APYFF+APAAT+RFT+LSST I
Sbjct: 59405 PSISGI*ISMKHASN*WGGLSIVMASIPLLTQTTPAPYFFKAPAATVRFTLLSSTIRIFL 59584
Query: 393 L*RRLPSVELPTTPFLQCSLSIPPNALCSET*SMLARSGFIITFKL 530
RR + +PF L P ALCSE S+LA GF+ T L
Sbjct: 59585 PERRCLRDKP*CSPFRGFPLFPTPIALCSEAASILALKGFVTTVGL 59722
Score = 70.7 bits (148), Expect(3) = 2e-061
Identities = 28/35 (80%), Positives = 31/35 (88%)
Frame = -3 / -1
Query: 106 PILAMTADVIQATHEQCLKSEMDGYVSKPFEGEQL 2
PILAMTADV+QATH++C K MDGYVSKPFE EQL
Sbjct: 58942 PILAMTADVMQATHQECSKCGMDGYVSKPFEAEQL 58838
Score = 56.5 bits (117), Expect(3) = 2e-061
Identities = 22/62 (35%), Positives = 38/62 (61%)
Frame = -2 / -2
Query: 797 KVTKYHLQRLGVQTELTTDLDQYISKNNCGSQIAKLVLIDKETWLKESHSMPLLVTKLRN 618
+V++YH+QRLGV+ E+ +DL Q +S G ++LI++E W ++S V +R
Sbjct: 60003 EVSRYHIQRLGVRVEIVSDLKQGLSTVTDGDASVNMILIEQEVWDRDSSISSHFVNNIRK 59824
Query: 617 IV 612
+V
Sbjct: 59823 VV 59818
Score = 49.2 bits (101), Expect(3) = 2e-030
Identities = 21/35 (60%), Positives = 25/35 (71%)
Frame = +3 / +3
Query: 3 NCSPSNGLET*PSISDFKHCSCVAWITSAVMANIG 107
+CS SNG ET PSI +H CVA ITSAV+A +G
Sbjct: 58839 SCSASNGFETYPSIPHLEHSWCVACITSAVIAKMG 58943
Score = 35.0 bits (70), Expect(3) = 2e-030
Identities = 23/61 (37%), Positives = 29/61 (47%)
Frame = +1 / +2
Query: 613 TMFLNLVTKRGMEWDSFSQVSLSMSTSFAICDPQLFLEIY*SRSVVNSV*TPSLCK*YLV 792
T FL L TK +S SQ S S+ P + ++ +S S TPSLC *YL
Sbjct: 59819 TTFLMLFTKCDEILESLSQTSCSIRIILTDASPSVTVDKPCFKSETISTRTPSLCM*YLD 59998
Query: 793 T 795
T
Sbjct: 59999 T 60001
>gb|AC147538.16| Medicago truncatula clone mth2-135c7, WORKING DRAFT SEQUENCE, 3 ordered
pieces
Length = 133436
Score = 167 bits (360), Expect(3) = 2e-061
Identities = 76/129 (58%), Positives = 92/129 (71%)
Frame = -3 / -1
Query: 595 KLFLLENPKSSVKSNSHIFREYNLNVIMKPLRASMLQVSLQRALGGIDKLHCRNGVVGNS 416
KLF+L N SS ++ S +N V+ KPLRASML SLQRA+G +K + RNG
Sbjct: 59519 KLFILVNSSSSFRAGSTASCLHNPTVVTKPLRASMLAASLQRAMGVGNKGNPRNGEHQGL 59340
Query: 415 TLGSLLHKKRIIVVDDNIVNLKVAAGALKKYGAEVTCADSGKEAITLLKPPHNFDACFMD 236
+L LL ++I++VDDN VN VAAGALKKYGA V C SG EAIT+L+PPH FDACFMD
Sbjct: 59339 SLKHLLSGRKILIVDDNSVNRTVAAGALKKYGAGVVCVSSGIEAITMLRPPHQFDACFMD 59160
Query: 235 IQMPEMDGF 209
IQMPEMDG+
Sbjct: 59159 IQMPEMDGY 59133
Score = 106 bits (226), Expect(3) = 2e-030
Identities = 57/106 (53%), Positives = 66/106 (62%)
Frame = +3 / +1
Query: 213 PSISGICMSMKQASKL*GGFSSVIASFPLSAQVTSAPYFFRAPAATLRFTILSSTTMIRF 392
PSISGI +SMK AS GG S V+AS PL Q T APYFF+APAAT+RFT+LSST I
Sbjct: 59137 PSISGI*ISMKHASN*WGGLSIVMASIPLLTQTTPAPYFFKAPAATVRFTLLSSTIRIFL 59316
Query: 393 L*RRLPSVELPTTPFLQCSLSIPPNALCSET*SMLARSGFIITFKL 530
RR + +PF L P ALCSE S+LA GF+ T L
Sbjct: 59317 PERRCLRDKP*CSPFRGFPLFPTPIALCSEAASILALKGFVTTVGL 59454
Score = 70.7 bits (148), Expect(3) = 2e-061
Identities = 28/35 (80%), Positives = 31/35 (88%)
Frame = -3 / -3
Query: 106 PILAMTADVIQATHEQCLKSEMDGYVSKPFEGEQL 2
PILAMTADV+QATH++C K MDGYVSKPFE EQL
Sbjct: 58674 PILAMTADVMQATHQECSKCGMDGYVSKPFEAEQL 58570
Score = 56.5 bits (117), Expect(3) = 2e-061
Identities = 22/62 (35%), Positives = 38/62 (61%)
Frame = -2 / -1
Query: 797 KVTKYHLQRLGVQTELTTDLDQYISKNNCGSQIAKLVLIDKETWLKESHSMPLLVTKLRN 618
+V++YH+QRLGV+ E+ +DL Q +S G ++LI++E W ++S V +R
Sbjct: 59735 EVSRYHIQRLGVRVEIVSDLKQGLSTVTDGDASVNMILIEQEVWDRDSSISSHFVNNIRK 59556
Query: 617 IV 612
+V
Sbjct: 59555 VV 59550
Score = 49.2 bits (101), Expect(3) = 2e-030
Identities = 21/35 (60%), Positives = 25/35 (71%)
Frame = +3 / +2
Query: 3 NCSPSNGLET*PSISDFKHCSCVAWITSAVMANIG 107
+CS SNG ET PSI +H CVA ITSAV+A +G
Sbjct: 58571 SCSASNGFETYPSIPHLEHSWCVACITSAVIAKMG 58675
Score = 35.0 bits (70), Expect(3) = 2e-030
Identities = 23/61 (37%), Positives = 29/61 (47%)
Frame = +1 / +1
Query: 613 TMFLNLVTKRGMEWDSFSQVSLSMSTSFAICDPQLFLEIY*SRSVVNSV*TPSLCK*YLV 792
T FL L TK +S SQ S S+ P + ++ +S S TPSLC *YL
Sbjct: 59551 TTFLMLFTKCDEILESLSQTSCSIRIILTDASPSVTVDKPCFKSETISTRTPSLCM*YLD 59730
Query: 793 T 795
T
Sbjct: 59731 T 59733
>gb|AC170800.5| Medicago truncatula clone mth2-32n7, complete sequence
Length = 99712
Score = 124 bits (266), Expect(4) = 3e-043
Identities = 49/80 (61%), Positives = 65/80 (81%)
Frame = -3 / +3
Query: 424 GNSTLGSLLHKKRIIVVDDNIVNLKVAAGALKKYGAEVTCADSGKEAITLLKPPHNFDAC 245
G++++ SLL K+I+VVDDN+VN +VAAGALK +GA+V CADSGK A+ +L+ PH FDAC
Sbjct: 42894 GSTSVRSLLFGKKILVVDDNVVNRRVAAGALKNFGADVKCADSGKAALEMLQFPHKFDAC 43073
Query: 244 FMDIQMPEMDGFEATKRIRV 185
FMDIQMPEMDG+ R+ +
Sbjct: 43074 FMDIQMPEMDGYVCLHRLLI 43133
Score = 90.9 bits (192), Expect(2) = 2e-026
Identities = 50/88 (56%), Positives = 55/88 (62%)
Frame = +3 / -1
Query: 213 PSISGICMSMKQASKL*GGFSSVIASFPLSAQVTSAPYFFRAPAATLRFTILSSTTMIRF 392
PSISGI +SMKQAS L*G S A+ PLSA TSAP FF+APAATLRFT LSSTT I F
Sbjct: 43105 PSISGI*ISMKQASNL*GN*SISRAALPLSAHFTSAPKFFKAPAATLRFTTLSSTTKIFF 42926
Query: 393 L*RRLPSVELPTTPFLQCSLSIPPNALC 476
RRL + P L +P C
Sbjct: 42925 PNRRLRTEVEPLGISFPSCLFLPVPKTC 42842
Score = 68.9 bits (144), Expect(4) = 3e-043
Identities = 28/39 (71%), Positives = 32/39 (82%)
Frame = -3 / +2
Query: 118 QWRTPILAMTADVIQATHEQCLKSEMDGYVSKPFEGEQL 2
++ PILAMTADVI AT+E+CLK MDGYVSKPFE E L
Sbjct: 43577 EFHLPILAMTADVIHATYEECLKCGMDGYVSKPFEEENL 43693
Score = 58.8 bits (122), Expect(2) = 2e-026
Identities = 26/33 (78%), Positives = 26/33 (78%)
Frame = +3 / -2
Query: 9 SPSNGLET*PSISDFKHCSCVAWITSAVMANIG 107
S S G ET*PSI FKH S VAWITSAVMANIG
Sbjct: 43686 SSSKGFET*PSIPHFKHSSYVAWITSAVMANIG 43588
Score = 32.7 bits (65), Expect(4) = 3e-043
Identities = 14/20 (70%), Positives = 16/20 (80%)
Frame = -3 / +3
Query: 520 VIMKPLRASMLQVSLQRALG 461
VIMKPLRASM+ LQ+ LG
Sbjct: 42795 VIMKPLRASMVGACLQQVLG 42854
Score = 31.8 bits (63), Expect(4) = 3e-043
Identities = 10/24 (41%), Positives = 19/24 (79%)
Frame = -2 / +1
Query: 794 VTKYHLQRLGVQTELTTDLDQYIS 723
VT+YHL+RLG+Q ++ +++ +S
Sbjct: 42241 VTRYHLKRLGIQVKVANAINKAVS 42312
>gb|AC141922.19| Medicago truncatula clone mth2-9n11, complete sequence
Length = 107448
Score = 124 bits (266), Expect(4) = 4e-043
Identities = 49/80 (61%), Positives = 65/80 (81%)
Frame = -3 / +3
Query: 424 GNSTLGSLLHKKRIIVVDDNIVNLKVAAGALKKYGAEVTCADSGKEAITLLKPPHNFDAC 245
G++++ SLL K+I+VVDDN+VN +VAAGALK +GA+V CADSGK A+ +L+ PH FDAC
Sbjct: 53376 GSTSVRSLLFGKKILVVDDNVVNRRVAAGALKNFGADVKCADSGKAALEMLQFPHKFDAC 53555
Query: 244 FMDIQMPEMDGFEATKRIRV 185
FMDIQMPEMDG+ R+ +
Sbjct: 53556 FMDIQMPEMDGYVCLHRLLI 53615
Score = 90.9 bits (192), Expect(2) = 3e-026
Identities = 50/88 (56%), Positives = 55/88 (62%)
Frame = +3 / -3
Query: 213 PSISGICMSMKQASKL*GGFSSVIASFPLSAQVTSAPYFFRAPAATLRFTILSSTTMIRF 392
PSISGI +SMKQAS L*G S A+ PLSA TSAP FF+APAATLRFT LSSTT I F
Sbjct: 53587 PSISGI*ISMKQASNL*GN*SISRAALPLSAHFTSAPKFFKAPAATLRFTTLSSTTKIFF 53408
Query: 393 L*RRLPSVELPTTPFLQCSLSIPPNALC 476
RRL + P L +P C
Sbjct: 53407 PNRRLRTEVEPLGISFPSCLFLPVPKTC 53324
Score = 68.9 bits (144), Expect(4) = 4e-043
Identities = 28/39 (71%), Positives = 32/39 (82%)
Frame = -3 / +2
Query: 118 QWRTPILAMTADVIQATHEQCLKSEMDGYVSKPFEGEQL 2
++ PILAMTADVI AT+E+CLK MDGYVSKPFE E L
Sbjct: 54059 EFHLPILAMTADVIHATYEECLKCGMDGYVSKPFEEENL 54175
Score = 58.8 bits (122), Expect(2) = 3e-026
Identities = 26/33 (78%), Positives = 26/33 (78%)
Frame = +3 / -1
Query: 9 SPSNGLET*PSISDFKHCSCVAWITSAVMANIG 107
S S G ET*PSI FKH S VAWITSAVMANIG
Sbjct: 54168 SSSKGFET*PSIPHFKHSSYVAWITSAVMANIG 54070
Score = 32.7 bits (65), Expect(4) = 4e-043
Identities = 14/20 (70%), Positives = 16/20 (80%)
Frame = -3 / +3
Query: 520 VIMKPLRASMLQVSLQRALG 461
VIMKPLRASM+ LQ+ LG
Sbjct: 53277 VIMKPLRASMVGACLQQVLG 53336
Score = 31.8 bits (63), Expect(4) = 4e-043
Identities = 10/24 (41%), Positives = 19/24 (79%)
Frame = -2 / +1
Query: 794 VTKYHLQRLGVQTELTTDLDQYIS 723
VT+YHL+RLG+Q ++ +++ +S
Sbjct: 52723 VTRYHLKRLGIQVKVANAINKAVS 52794
>gb|AC144931.28| Medicago truncatula clone mth2-22b18, complete sequence
Length = 122821
Score = 124 bits (266), Expect(4) = 5e-043
Identities = 49/80 (61%), Positives = 65/80 (81%)
Frame = -3 / +2
Query: 424 GNSTLGSLLHKKRIIVVDDNIVNLKVAAGALKKYGAEVTCADSGKEAITLLKPPHNFDAC 245
G++++ SLL K+I+VVDDN+VN +VAAGALK +GA+V CADSGK A+ +L+ PH FDAC
Sbjct: 24911 GSTSVRSLLFGKKILVVDDNVVNRRVAAGALKNFGADVKCADSGKAALEMLQFPHKFDAC 25090
Query: 244 FMDIQMPEMDGFEATKRIRV 185
FMDIQMPEMDG+ R+ +
Sbjct: 25091 FMDIQMPEMDGYVCLHRLLI 25150
Score = 90.9 bits (192), Expect(2) = 3e-026
Identities = 50/88 (56%), Positives = 55/88 (62%)
Frame = +3 / -2
Query: 213 PSISGICMSMKQASKL*GGFSSVIASFPLSAQVTSAPYFFRAPAATLRFTILSSTTMIRF 392
PSISGI +SMKQAS L*G S A+ PLSA TSAP FF+APAATLRFT LSSTT I F
Sbjct: 25122 PSISGI*ISMKQASNL*GN*SISRAALPLSAHFTSAPKFFKAPAATLRFTTLSSTTKIFF 24943
Query: 393 L*RRLPSVELPTTPFLQCSLSIPPNALC 476
RRL + P L +P C
Sbjct: 24942 PNRRLRTEVEPLGISFPSCLFLPVPKTC 24859
Score = 68.9 bits (144), Expect(4) = 5e-043
Identities = 28/39 (71%), Positives = 32/39 (82%)
Frame = -3 / +1
Query: 118 QWRTPILAMTADVIQATHEQCLKSEMDGYVSKPFEGEQL 2
++ PILAMTADVI AT+E+CLK MDGYVSKPFE E L
Sbjct: 25594 EFHLPILAMTADVIHATYEECLKCGMDGYVSKPFEEENL 25710
Score = 58.8 bits (122), Expect(2) = 3e-026
Identities = 26/33 (78%), Positives = 26/33 (78%)
Frame = +3 / -3
Query: 9 SPSNGLET*PSISDFKHCSCVAWITSAVMANIG 107
S S G ET*PSI FKH S VAWITSAVMANIG
Sbjct: 25703 SSSKGFET*PSIPHFKHSSYVAWITSAVMANIG 25605
Score = 32.7 bits (65), Expect(4) = 5e-043
Identities = 14/20 (70%), Positives = 16/20 (80%)
Frame = -3 / +2
Query: 520 VIMKPLRASMLQVSLQRALG 461
VIMKPLRASM+ LQ+ LG
Sbjct: 24812 VIMKPLRASMVGACLQQVLG 24871
Score = 31.8 bits (63), Expect(4) = 5e-043
Identities = 10/24 (41%), Positives = 19/24 (79%)
Frame = -2 / +3
Query: 794 VTKYHLQRLGVQTELTTDLDQYIS 723
VT+YHL+RLG+Q ++ +++ +S
Sbjct: 24258 VTRYHLKRLGIQVKVANAINKAVS 24329
>gb|AC142094.12| Medicago truncatula clone mth2-34h6, complete sequence
Length = 151594
Score = 124 bits (266), Expect(4) = 1e-042
Identities = 49/80 (61%), Positives = 65/80 (81%)
Frame = -3 / -2
Query: 424 GNSTLGSLLHKKRIIVVDDNIVNLKVAAGALKKYGAEVTCADSGKEAITLLKPPHNFDAC 245
G++++ SLL K+I+VVDDN+VN +VAAGALK +GA+V CADSGK A+ +L+ PH FDAC
Sbjct: 126684 GSTSVRSLLFGKKILVVDDNVVNRRVAAGALKNFGADVKCADSGKAALEMLQFPHKFDAC 126505
Query: 244 FMDIQMPEMDGFEATKRIRV 185
FMDIQMPEMDG+ R+ +
Sbjct: 126504 FMDIQMPEMDGYVCLHRLLI 126445
Score = 90.9 bits (192), Expect(2) = 4e-026
Identities = 50/88 (56%), Positives = 55/88 (62%)
Frame = +3 / +2
Query: 213 PSISGICMSMKQASKL*GGFSSVIASFPLSAQVTSAPYFFRAPAATLRFTILSSTTMIRF 392
PSISGI +SMKQAS L*G S A+ PLSA TSAP FF+APAATLRFT LSSTT I F
Sbjct: 126473 PSISGI*ISMKQASNL*GN*SISRAALPLSAHFTSAPKFFKAPAATLRFTTLSSTTKIFF 126652
Query: 393 L*RRLPSVELPTTPFLQCSLSIPPNALC 476
RRL + P L +P C
Sbjct: 126653 PNRRLRTEVEPLGISFPSCLFLPVPKTC 126736
Score = 68.9 bits (144), Expect(4) = 1e-042
Identities = 28/39 (71%), Positives = 32/39 (82%)
Frame = -3 / -1
Query: 118 QWRTPILAMTADVIQATHEQCLKSEMDGYVSKPFEGEQL 2
++ PILAMTADVI AT+E+CLK MDGYVSKPFE E L
Sbjct: 126001 EFHLPILAMTADVIHATYEECLKCGMDGYVSKPFEEENL 125885
Score = 58.8 bits (122), Expect(2) = 4e-026
Identities = 26/33 (78%), Positives = 26/33 (78%)
Frame = +3 / +3
Query: 9 SPSNGLET*PSISDFKHCSCVAWITSAVMANIG 107
S S G ET*PSI FKH S VAWITSAVMANIG
Sbjct: 125892 SSSKGFET*PSIPHFKHSSYVAWITSAVMANIG 125990
Score = 32.7 bits (65), Expect(4) = 1e-042
Identities = 14/20 (70%), Positives = 16/20 (80%)
Frame = -3 / -2
Query: 520 VIMKPLRASMLQVSLQRALG 461
VIMKPLRASM+ LQ+ LG
Sbjct: 126783 VIMKPLRASMVGACLQQVLG 126724
Score = 31.8 bits (63), Expect(4) = 1e-042
Identities = 10/24 (41%), Positives = 19/24 (79%)
Frame = -2 / -3
Query: 794 VTKYHLQRLGVQTELTTDLDQYIS 723
VT+YHL+RLG+Q ++ +++ +S
Sbjct: 127337 VTRYHLKRLGIQVKVANAINKAVS 127266
>gb|CX520904.1|CX520904 s13dNF58E12VI099_449594 Virus-Infected Leaves Medicago truncatula
cDNA, mRNA sequence
Length = 602
Score = 155 bits (334), Expect(2) = 2e-042
Identities = 65/98 (66%), Positives = 75/98 (76%)
Frame = -3 / +3
Query: 418 STLGSLLHKKRIIVVDDNIVNLKVAAGALKKYGAEVTCADSGKEAITLLKPPHNFDACFM 239
S L +LL KRI+VVDDN VN KVA G L+KYGA VTC D GK A+ +LKPPHNFDACFM
Sbjct: 237 SQLENLLIHKRILVVDDNAVNRKVAEGVLRKYGAVVTCVDGGKAALKVLKPPHNFDACFM 416
Query: 238 DIQMPEMDGFEATKRIRVMERDLNEQIERGEAPPECVG 125
D+QMPEMDGFE T+RIR ME ++NE+I GE E G
Sbjct: 417 DLQMPEMDGFEVTRRIRYMENEVNEKIACGEELAEMFG 530
Score = 109 bits (233), Expect(2) = 4e-025
Identities = 53/88 (60%), Positives = 61/88 (69%)
Frame = +3 / -2
Query: 126 PTHSGGASPRSICSFRSLSITLILFVASNPSISGICMSMKQASKL*GGFSSVIASFPLSA 305
P S +SP++I SF S SI I V S PSISGI SMKQASKL GG S++ A+ P S
Sbjct: 529 PNISANSSPQAIFSFTSFSIYRIRLVTSKPSISGIWRSMKQASKLCGGLSTLRAALPPST 350
Query: 306 QVTSAPYFFRAPAATLRFTILSSTTMIR 389
QVT+APYF R P+AT FT LSSTT IR
Sbjct: 349 QVTTAPYFRRTPSATFLFTALSSTTKIR 266
Score = 38.6 bits (78), Expect(2) = 2e-042
Identities = 14/23 (60%), Positives = 17/23 (73%)
Frame = -3 / +3
Query: 124 VRQWRTPILAMTADVIQATHEQC 56
+ W PILAMTADV QA+ E+C
Sbjct: 534 ISYWHIPILAMTADVTQASXEEC 602
Score = 26.7 bits (52), Expect(2) = 4e-025
Identities = 11/23 (47%), Positives = 12/23 (52%)
Frame = +2 / -1
Query: 56 ALFMCRLDNICCHG*YWSSPLPN 124
A F L I CH *YW P+ N
Sbjct: 602 AFFX*CLSYISCHS*YWYVPIRN 534
>gb|AC174287.2| Medicago truncatula clone mth2-32a8, WORKING DRAFT SEQUENCE, 8
unordered pieces
Length = 107645
Score = 123 bits (263), Expect(3) = 4e-039
Identities = 50/69 (72%), Positives = 56/69 (81%)
Frame = -3 / -3
Query: 418 STLGSLLHKKRIIVVDDNIVNLKVAAGALKKYGAEVTCADSGKEAITLLKPPHNFDACFM 239
S L +LL KRI+VVDDN VN KVA G L+KYGA VTC D GK A+ +LKPPHNFDACFM
Sbjct: 28878 SQLENLLIHKRILVVDDNAVNRKVAEGVLRKYGAVVTCVDGGKAALKVLKPPHNFDACFM 28699
Query: 238 DIQMPEMDG 212
D+QMPEMDG
Sbjct: 28698 DLQMPEMDG 28672
Score = 65.2 bits (136), Expect(3) = 4e-039
Identities = 26/41 (63%), Positives = 31/41 (75%)
Frame = -3 / -3
Query: 124 VRQWRTPILAMTADVIQATHEQCLKSEMDGYVSKPFEGEQL 2
+ W PILAMTADV QA++E+C K MD +VSKPFE EQL
Sbjct: 28488 ISYWHIPILAMTADVTQASNEECKKCGMDDFVSKPFEEEQL 28366
Score = 30.4 bits (60), Expect(3) = 4e-039
Identities = 10/20 (50%), Positives = 16/20 (80%)
Frame = -2 / -1
Query: 797 KVTKYHLQRLGVQTELTTDL 738
+VT+YHLQRLG+ ++ + L
Sbjct: 29378 EVTRYHLQRLGMSVDVNSSL 29319
>emb|CR955006.2| Medicago truncatula chromosome 5 clone mth2-5i4, COMPLETE SEQUENCE
Length = 108871
Score = 123 bits (263), Expect(3) = 5e-039
Identities = 50/69 (72%), Positives = 56/69 (81%)
Frame = -3 / -1
Query: 418 STLGSLLHKKRIIVVDDNIVNLKVAAGALKKYGAEVTCADSGKEAITLLKPPHNFDACFM 239
S L +LL KRI+VVDDN VN KVA G L+KYGA VTC D GK A+ +LKPPHNFDACFM
Sbjct: 106486 SQLENLLIHKRILVVDDNAVNRKVAEGVLRKYGAVVTCVDGGKAALKVLKPPHNFDACFM 106307
Query: 238 DIQMPEMDG 212
D+QMPEMDG
Sbjct: 106306 DLQMPEMDG 106280
Score = 65.2 bits (136), Expect(3) = 5e-039
Identities = 26/41 (63%), Positives = 31/41 (75%)
Frame = -3 / -1
Query: 124 VRQWRTPILAMTADVIQATHEQCLKSEMDGYVSKPFEGEQL 2
+ W PILAMTADV QA++E+C K MD +VSKPFE EQL
Sbjct: 106096 ISYWHIPILAMTADVTQASNEECKKCGMDDFVSKPFEEEQL 105974
Score = 30.4 bits (60), Expect(3) = 5e-039
Identities = 10/20 (50%), Positives = 16/20 (80%)
Frame = -2 / -2
Query: 797 KVTKYHLQRLGVQTELTTDL 738
+VT+YHLQRLG+ ++ + L
Sbjct: 106986 EVTRYHLQRLGMSVDVNSSL 106927
>gb|AC160840.20| Medicago truncatula clone mth2-27o21, WORKING DRAFT SEQUENCE, 2 ordered
pieces
Length = 119300
Score = 123 bits (263), Expect(3) = 5e-039
Identities = 50/69 (72%), Positives = 56/69 (81%)
Frame = -3 / -1
Query: 418 STLGSLLHKKRIIVVDDNIVNLKVAAGALKKYGAEVTCADSGKEAITLLKPPHNFDACFM 239
S L +LL KRI+VVDDN VN KVA G L+KYGA VTC D GK A+ +LKPPHNFDACFM
Sbjct: 111749 SQLENLLIHKRILVVDDNAVNRKVAEGVLRKYGAVVTCVDGGKAALKVLKPPHNFDACFM 111570
Query: 238 DIQMPEMDG 212
D+QMPEMDG
Sbjct: 111569 DLQMPEMDG 111543
Score = 65.2 bits (136), Expect(3) = 5e-039
Identities = 26/41 (63%), Positives = 31/41 (75%)
Frame = -3 / -1
Query: 124 VRQWRTPILAMTADVIQATHEQCLKSEMDGYVSKPFEGEQL 2
+ W PILAMTADV QA++E+C K MD +VSKPFE EQL
Sbjct: 111359 ISYWHIPILAMTADVTQASNEECKKCGMDDFVSKPFEEEQL 111237
Score = 30.4 bits (60), Expect(3) = 5e-039
Identities = 10/20 (50%), Positives = 16/20 (80%)
Frame = -2 / -2
Query: 797 KVTKYHLQRLGVQTELTTDL 738
+VT+YHLQRLG+ ++ + L
Sbjct: 112249 EVTRYHLQRLGMSVDVNSSL 112190
>emb|CT571263.1| Medicago truncatula chromosome 5 clone mte1-7c20, COMPLETE SEQUENCE
Length = 127003
Score = 123 bits (263), Expect(3) = 6e-039
Identities = 50/69 (72%), Positives = 56/69 (81%)
Frame = -3 / +3
Query: 418 STLGSLLHKKRIIVVDDNIVNLKVAAGALKKYGAEVTCADSGKEAITLLKPPHNFDACFM 239
S L +LL KRI+VVDDN VN KVA G L+KYGA VTC D GK A+ +LKPPHNFDACFM
Sbjct: 118860 SQLENLLIHKRILVVDDNAVNRKVAEGVLRKYGAVVTCVDGGKAALKVLKPPHNFDACFM 119039
Query: 238 DIQMPEMDG 212
D+QMPEMDG
Sbjct: 119040 DLQMPEMDG 119066
Score = 65.2 bits (136), Expect(3) = 6e-039
Identities = 26/41 (63%), Positives = 31/41 (75%)
Frame = -3 / +3
Query: 124 VRQWRTPILAMTADVIQATHEQCLKSEMDGYVSKPFEGEQL 2
+ W PILAMTADV QA++E+C K MD +VSKPFE EQL
Sbjct: 119250 ISYWHIPILAMTADVTQASNEECKKCGMDDFVSKPFEEEQL 119372
Score = 30.4 bits (60), Expect(3) = 6e-039
Identities = 10/20 (50%), Positives = 16/20 (80%)
Frame = -2 / +1
Query: 797 KVTKYHLQRLGVQTELTTDL 738
+VT+YHLQRLG+ ++ + L
Sbjct: 118360 EVTRYHLQRLGMSVDVNSSL 118419
>gb|CX527617.1|CX527617 s13dNF45E07AT055_515746 Aphid-Infected Shoots Medicago truncatula
cDNA, mRNA sequence
Length = 600
Score = 133 bits (286), Expect(2) = 4e-034
Identities = 53/77 (68%), Positives = 67/77 (87%)
Frame = -3 / +1
Query: 424 GNSTLGSLLHKKRIIVVDDNIVNLKVAAGALKKYGAEVTCADSGKEAITLLKPPHNFDAC 245
G++++ SLL K+I+VVDDN+VN +VAAGALK +GA+V CADSGK A+ +L+ PH FDAC
Sbjct: 370 GSTSVRSLLFGKKILVVDDNVVNRRVAAGALKNFGADVKCADSGKAALEMLQFPHKFDAC 549
Query: 244 FMDIQMPEMDGFEATKR 194
FMDIQMPEMDGFEAT+R
Sbjct: 550 FMDIQMPEMDGFEATRR 600
Score = 32.7 bits (65), Expect(2) = 4e-034
Identities = 14/20 (70%), Positives = 16/20 (80%)
Frame = -3 / +1
Query: 520 VIMKPLRASMLQVSLQRALG 461
VIMKPLRASM+ LQ+ LG
Sbjct: 271 VIMKPLRASMVGACLQQVLG 330
>gb|CX532999.1|CX532999 s13dNF89B04MJ029_272276 Methyl Jasmonate-Elicited Root Cell
Suspension Culture Medicago truncatula cDNA, mRNA
sequence
Length = 611
Score = 107 bits (228), Expect(2) = 3e-033
Identities = 44/65 (67%), Positives = 52/65 (80%)
Frame = -3 / +3
Query: 421 NSTLGSLLHKKRIIVVDDNIVNLKVAAGALKKYGAEVTCADSGKEAITLLKPPHNFDACF 242
N +L LL ++I++VDDN VN VAAGALKKYGA+V C SGK+AI+ LKPPH FDACF
Sbjct: 417 NLSLSHLLVGRKILIVDDNGVNRAVAAGALKKYGADVVCVSSGKDAISSLKPPHQFDACF 596
Query: 241 MDIQM 227
MDIQM
Sbjct: 597 MDIQM 611
Score = 58.3 bits (121), Expect(2) = 3e-033
Identities = 21/48 (43%), Positives = 35/48 (72%)
Frame = -2 / +3
Query: 797 KVTKYHLQRLGVQTELTTDLDQYISKNNCGSQIAKLVLIDKETWLKES 654
KV++YH+QRLG+ E +DL+Q +S N G+ + +VLI++E W ++S
Sbjct: 45 KVSQYHIQRLGIHVEKVSDLNQGLSTINKGNAVINMVLIEQEVWYRDS 188
>gb|AC146785.12| Medicago truncatula clone mth2-4h4, complete sequence
Length = 149005
Score = 122 bits (261), Expect(2) = 6e-031
Identities = 49/72 (68%), Positives = 60/72 (83%)
Frame = -3 / +2
Query: 424 GNSTLGSLLHKKRIIVVDDNIVNLKVAAGALKKYGAEVTCADSGKEAITLLKPPHNFDAC 245
G+S SLLH K+I+VVDDN VNL+VAA ALKK+GA+V C +SGK A+ +L+ PH FDAC
Sbjct: 8213 GSSFQQSLLHYKKILVVDDNCVNLRVAADALKKFGADVKCVESGKAALEMLQLPHKFDAC 8392
Query: 244 FMDIQMPEMDGF 209
FMDIQMPEMDG+
Sbjct: 8393 FMDIQMPEMDGY 8428
Score = 33.1 bits (66), Expect(2) = 6e-031
Identities = 14/20 (70%), Positives = 17/20 (85%)
Frame = -3 / +3
Query: 520 VIMKPLRASMLQVSLQRALG 461
+IMKPLRASM+ V LQ+ LG
Sbjct: 8121 MIMKPLRASMVVVCLQQVLG 8180
>emb|CR932957.2| Medicago truncatula chromosome 5 clone mth2-58c3, COMPLETE SEQUENCE
Length = 136080
Score = 122 bits (261), Expect(2) = 6e-031
Identities = 49/72 (68%), Positives = 60/72 (83%)
Frame = -3 / -3
Query: 424 GNSTLGSLLHKKRIIVVDDNIVNLKVAAGALKKYGAEVTCADSGKEAITLLKPPHNFDAC 245
G+S SLLH K+I+VVDDN VNL+VAA ALKK+GA+V C +SGK A+ +L+ PH FDAC
Sbjct: 4483 GSSFQQSLLHYKKILVVDDNCVNLRVAADALKKFGADVKCVESGKAALEMLQLPHKFDAC 4304
Query: 244 FMDIQMPEMDGF 209
FMDIQMPEMDG+
Sbjct: 4303 FMDIQMPEMDGY 4268
Score = 33.1 bits (66), Expect(2) = 6e-031
Identities = 14/20 (70%), Positives = 17/20 (85%)
Frame = -3 / -1
Query: 520 VIMKPLRASMLQVSLQRALG 461
+IMKPLRASM+ V LQ+ LG
Sbjct: 4575 MIMKPLRASMVVVCLQQVLG 4516
>gb|CX531807.1|CX531807 s13dNF87G02MJ008_257556 Methyl Jasmonate-Elicited Root Cell
Suspension Culture Medicago truncatula cDNA, mRNA
sequence
Length = 651
Score = 77.6 bits (163), Expect(2) = 2e-024
Identities = 33/53 (62%), Positives = 41/53 (77%)
Frame = -3 / +1
Query: 421 NSTLGSLLHKKRIIVVDDNIVNLKVAAGALKKYGAEVTCADSGKEAITLLKPP 263
N +L LL ++I++VDDN VN VAAGALKKYGA+V C SGK+AI+ LKPP
Sbjct: 493 NLSLSHLLVGRKILIVDDNGVNRAVAAGALKKYGADVVCVXSGKDAISSLKPP 651
Score = 58.3 bits (121), Expect(2) = 2e-024
Identities = 21/48 (43%), Positives = 35/48 (72%)
Frame = -2 / +1
Query: 797 KVTKYHLQRLGVQTELTTDLDQYISKNNCGSQIAKLVLIDKETWLKES 654
KV++YH+QRLG+ E +DL+Q +S N G+ + +VLI++E W ++S
Sbjct: 121 KVSQYHIQRLGIHVEKVSDLNQGLSTINKGNAVINMVLIEQEVWYRDS 264
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 914,666,576
Number of Sequences: 392609
Number of extensions: 14379113
Number of successful extensions: 55575
Number of sequences better than 1.0e-020: 22
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 26595166895
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)