TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3023930.2.1
(544 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AJ496918.1|AJ496918 AJ496918 MTFLOW Medicago truncatula ... 73 8e-023
gb|AJ496970.1|AJ496970 AJ496970 MTFLOW Medicago truncatula ... 73 8e-023
gb|AC141107.5| Medicago truncatula clone mth2-34a12, comple... 79 2e-022
>gb|AJ496918.1|AJ496918 AJ496918 MTFLOW Medicago truncatula cDNA clone mt--abc955101f08,
mRNA sequence
Length = 642
Score = 73.0 bits (153), Expect(2) = 8e-023
Identities = 31/81 (38%), Positives = 48/81 (59%)
Frame = +1 / +1
Query: 1 YGCPNYWMLHFFKESSGAILHPTAIQVSSYDQLVASAITWQNAKDKSTYLRIKVVNFGNQ 180
YG P+YW+ + FKES+GA + +Q + L ASAI ++ ++K+TYL+IK+ N
Sbjct: 46 YGTPSYWVTYLFKESNGATFLNSTLQTTDPGTLAASAILVKDPQNKNTYLKIKIANMRKT 225
Query: 181 AVDLNISVAELATGVKKSGSK 243
VD IS+ A+ K +K
Sbjct: 226 QVDFKISIQGFASKNLKGSTK 288
Score = 54.7 bits (113), Expect(2) = 8e-023
Identities = 21/44 (47%), Positives = 30/44 (68%)
Frame = +1 / +1
Query: 259 SSSPLDENSFQQPEKVAPVSSPMANAGQQMGASVGPYSLTSFDL 390
+ + LDENSF +P+K+AP +SP+ N G +M V P SLT D+
Sbjct: 298 TGNELDENSFAEPKKIAPQTSPLQNPGNEMNVIVQPTSLTILDM 429
>gb|AJ496970.1|AJ496970 AJ496970 MTFLOW Medicago truncatula cDNA clone mt--abc955102d10,
mRNA sequence
Length = 606
Score = 73.0 bits (153), Expect(2) = 8e-023
Identities = 31/81 (38%), Positives = 48/81 (59%)
Frame = +1 / +1
Query: 1 YGCPNYWMLHFFKESSGAILHPTAIQVSSYDQLVASAITWQNAKDKSTYLRIKVVNFGNQ 180
YG P+YW+ + FKES+GA + +Q + L ASAI ++ ++K+TYL+IK+ N
Sbjct: 10 YGTPSYWVTYLFKESNGATFLNSTLQTTDPGTLAASAILVKDPQNKNTYLKIKIANMRKT 189
Query: 181 AVDLNISVAELATGVKKSGSK 243
VD IS+ A+ K +K
Sbjct: 190 QVDFKISIQGFASKNLKGSTK 252
Score = 54.7 bits (113), Expect(2) = 8e-023
Identities = 21/44 (47%), Positives = 30/44 (68%)
Frame = +1 / +1
Query: 259 SSSPLDENSFQQPEKVAPVSSPMANAGQQMGASVGPYSLTSFDL 390
+ + LDENSF +P+K+AP +SP+ N G +M V P SLT D+
Sbjct: 262 TGNELDENSFAEPKKIAPQTSPLQNPGNEMNVIVQPTSLTILDM 393
>gb|AC141107.5| Medicago truncatula clone mth2-34a12, complete sequence
Length = 142659
Score = 79.0 bits (166), Expect(3) = 2e-022
Identities = 31/54 (57%), Positives = 41/54 (75%)
Frame = +1 / +2
Query: 1 YGCPNYWMLHFFKESSGAILHPTAIQVSSYDQLVASAITWQNAKDKSTYLRIKV 162
YG P+YWM FF ES+GA L +++Q ++ + LVASAITWQN+ DK Y+RIKV
Sbjct: 58730 YGTPSYWMQLFFSESNGATLLNSSLQTTASNSLVASAITWQNSVDKKNYIRIKV 58891
Score = 44.1 bits (90), Expect(3) = 2e-022
Identities = 18/32 (56%), Positives = 26/32 (81%)
Frame = +1 / +2
Query: 223 VKKSGSKQTVLTSSSPLDENSFQQPEKVAPVS 318
++ SGS +TVLTS++ +DENSF QP+KV +S
Sbjct: 59321 LQSSGSTKTVLTSTNLMDENSFSQPKKVP*IS 59416
Score = 38.2 bits (77), Expect(3) = 2e-022
Identities = 16/35 (45%), Positives = 22/35 (62%)
Frame = +1 / +1
Query: 301 KVAPVSSPMANAGQQMGASVGPYSLTSFDLLLEPS 405
+V P+ S + + G+ M V P+S TSFDLL E S
Sbjct: 59536 QVIPIQSLLQSVGKDMNVIVPPHSFTSFDLLKESS 59640
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 514,671,671
Number of Sequences: 392609
Number of extensions: 6206015
Number of successful extensions: 45146
Number of sequences better than 1.0e-020: 3
length of database: 147,244,331
effective HSP length: 53
effective length of database: 126,436,054
effective search space used: 16057378858
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)