TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3012915.2.2
(621 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG453428.1|BG453428 NF091G11LF1F1085 Developing leaf Med... 108 2e-037
gb|BG455193.1|BG455193 NF100G04PL1F1034 Phosphate starved l... 105 3e-028
gb|BF648216.1|BF648216 NF045D01EC1F1012 Elicited cell cultu... 88 2e-026
gb|CA917715.1|CA917715 EST641862 GPOD Medicago truncatula c... 109 5e-023
gb|AL379968.1|AL379968 MtBB48E05F1 MtBB Medicago truncatula... 105 5e-022
gb|AL379969.1|AL379969 MtBB48E05R1 MtBB Medicago truncatula... 73 2e-021
gb|AW687498.2|AW687498 NF010C04RT1F1033 Developing root Med... 75 3e-021
>gb|BG453428.1|BG453428 NF091G11LF1F1085 Developing leaf Medicago truncatula cDNA clone
NF091G11LF 5', mRNA sequence
Length = 638
Score = 108 bits (230), Expect(3) = 2e-037
Identities = 43/79 (54%), Positives = 56/79 (70%)
Frame = +2 / +3
Query: 95 APYHFDLSGTAFGRLAKPGRNDDLRRAGIIDIQFARVPCEFPGLKVGFHVEEGSTQVYFA 274
A YHFDLSG+AFG +A G +++LR AG I I+ RV C + G + FHV+ GS Q YFA
Sbjct: 18 AEYHFDLSGSAFGSMAVSGEDEELRNAGKIVIEHRRVQCNYGGSTIAFHVDSGSNQEYFA 197
Query: 275 VLVEYENGDGDVVQVDLME 331
L+EYE GDGD+ +V+L E
Sbjct: 198 ALIEYEEGDGDLNKVELKE 254
Score = 44.6 bits (91), Expect(3) = 2e-037
Identities = 17/31 (54%), Positives = 21/31 (67%)
Frame = +2 / +3
Query: 428 TRTDSGKTLVARDVIPLNWRPNTFYRSIVQY 520
T SGKT+VA +VIP W+P YRSIV +
Sbjct: 357 TTLKSGKTIVAHNVIPAGWKPGQTYRSIVNF 449
Score = 42.3 bits (86), Expect(3) = 2e-037
Identities = 14/30 (46%), Positives = 18/30 (60%)
Frame = +2 / +3
Query: 347 WTPMSQSWGSIWRLDSNHRLQPPFSIRTRT 436
W M QSWG++W+ D L+ P SIR T
Sbjct: 273 WDTMQQSWGAVWKFDKGAPLKAPISIRLTT 362
>gb|BG455193.1|BG455193 NF100G04PL1F1034 Phosphate starved leaf Medicago truncatula cDNA
clone NF100G04PL 5', mRNA sequence
Length = 654
Score = 105 bits (225), Expect(2) = 3e-028
Identities = 39/95 (41%), Positives = 56/95 (58%)
Frame = +2 / +2
Query: 104 HFDLSGTAFGRLAKPGRNDDLRRAGIIDIQFARVPCEFPGLKVGFHVEEGSTQVYFAVLV 283
HFDLSG AFG +A G N LR G I + + R C + G + F V EGST + ++LV
Sbjct: 206 HFDLSGAAFGHMAVHGENGQLRNRGEIQVLYRRTTCVYAGKNIAFQVNEGSTPFWLSLLV 385
Query: 284 EYENGDGDVVQVDLMEKRARRWTPMSQSWGSIWRL 388
E+E+GDGD+ + + E + W M+ WG+ W +
Sbjct: 386 EFEDGDGDISSMHIQEAGSSEWLQMNHLWGANWNI 490
Score = 40.5 bits (82), Expect(2) = 3e-028
Identities = 14/27 (51%), Positives = 21/27 (77%)
Frame = +2 / +2
Query: 404 LQPPFSIRTRTDSGKTLVARDVIPLNW 484
L+ PFS++ T +GK+L A+DVIP +W
Sbjct: 503 LRGPFSVKLSTSTGKSLTAKDVIPSHW 583
>gb|BF648216.1|BF648216 NF045D01EC1F1012 Elicited cell culture Medicago truncatula cDNA
clone NF045D01EC 5', mRNA sequence
Length = 596
Score = 87.7 bits (185), Expect(3) = 2e-026
Identities = 31/79 (39%), Positives = 49/79 (62%)
Frame = +2 / +3
Query: 146 PGRNDDLRRAGIIDIQFARVPCEFPGLKVGFHVEEGSTQVYFAVLVEYENGDGDVVQVDL 325
P R +LR G I + + R PC++PG K+ FHV EGST + ++LVE+E+ +GD+ + +
Sbjct: 36 PVRTVNLRNRGQIPVIYRRTPCKYPGKKIAFHVNEGSTPFWLSLLVEFEDAEGDIGTMHI 215
Query: 326 MEKRARRWTPMSQSWGSIW 382
E + W M+ WG+ W
Sbjct: 216 RENGSSEWLQMNHLWGANW 272
Score = 47.3 bits (97), Expect(3) = 2e-026
Identities = 17/39 (43%), Positives = 26/39 (66%)
Frame = +2 / +3
Query: 404 LQPPFSIRTRTDSGKTLVARDVIPLNWRPNTFYRSIVQY 520
L+ PFS++ + +G+TL ARDV+P NW P Y S + +
Sbjct: 291 LRGPFSVKLSSSTGRTLSARDVVPSNWVPKATYTSRLNF 407
Score = 23.1 bits (44), Expect(3) = 2e-026
Identities = 9/15 (60%), Positives = 10/15 (66%)
Frame = +2 / +2
Query: 113 LSGTAFGRLAKPGRN 157
LSG AFG +A G N
Sbjct: 2 LSGAAFGHMAISGEN 46
>gb|CA917715.1|CA917715 EST641862 GPOD Medicago truncatula cDNA clone GPOD-37E9, mRNA
sequence
Length = 769
Score = 109 bits (232), Expect = 5e-023
Identities = 40/95 (42%), Positives = 57/95 (60%)
Frame = +2 / +2
Query: 104 HFDLSGTAFGRLAKPGRNDDLRRAGIIDIQFARVPCEFPGLKVGFHVEEGSTQVYFAVLV 283
HFDLSG AFG +A G N LR G I + + R C + G + F V EGST + ++LV
Sbjct: 458 HFDLSGAAFGHMAVHGENGQLRNRGEIQVLYRRTTCVYAGKNIAFQVNEGSTPFWLSLLV 637
Query: 284 EYENGDGDVVQVDLMEKRARRWTPMSQSWGSIWRL 388
E+E+GDGD+ + + E + W M+ WG+IW +
Sbjct: 638 EFEDGDGDISSMHIQEAGSSEWLQMNHLWGAIWNM 742
>gb|AL379968.1|AL379968 MtBB48E05F1 MtBB Medicago truncatula cDNA clone MtBB48E05 T3, mRNA
sequence
Length = 476
Score = 105 bits (225), Expect = 5e-022
Identities = 39/95 (41%), Positives = 56/95 (58%)
Frame = +2 / +2
Query: 104 HFDLSGTAFGRLAKPGRNDDLRRAGIIDIQFARVPCEFPGLKVGFHVEEGSTQVYFAVLV 283
HFDLSG AFG +A G N LR G I + + R C + G + F V EGST + ++LV
Sbjct: 134 HFDLSGAAFGHMAVHGENGQLRNRGEIQVLYRRTTCVYAGKNIAFQVNEGSTPFWLSLLV 313
Query: 284 EYENGDGDVVQVDLMEKRARRWTPMSQSWGSIWRL 388
E+E+GDGD+ + + E + W M+ WG+ W +
Sbjct: 314 EFEDGDGDISSMHIQEAGSSEWLQMNHLWGANWNI 418
>gb|AL379969.1|AL379969 MtBB48E05R1 MtBB Medicago truncatula cDNA clone MtBB48E05 T7, mRNA
sequence
Length = 488
Score = 73.5 bits (154), Expect(2) = 2e-021
Identities = 25/71 (35%), Positives = 41/71 (57%)
Frame = +2 / +2
Query: 176 GIIDIQFARVPCEFPGLKVGFHVEEGSTQVYFAVLVEYENGDGDVVQVDLMEKRARRWTP 355
G I + + R C + G + F V EGST + ++LVE+E+GDGD+ + + E + W
Sbjct: 2 GEIQVLYRRTTCVYAGKNIAFQVNEGSTPFWLSLLVEFEDGDGDISSMHIQEAGSSEWLQ 181
Query: 356 MSQSWGSIWRL 388
M+ WG+ W +
Sbjct: 182 MNHLWGANWNI 214
Score = 49.6 bits (102), Expect(2) = 2e-021
Identities = 18/40 (45%), Positives = 28/40 (70%)
Frame = +2 / +2
Query: 404 LQPPFSIRTRTDSGKTLVARDVIPLNWRPNTFYRSIVQYS 523
L+ PFS++ T +GK+L A+DVIP +W P + Y S + +S
Sbjct: 227 LRGPFSVKLSTSTGKSLTAKDVIPSHWSPKSTYTSRLNFS 346
>gb|AW687498.2|AW687498 NF010C04RT1F1033 Developing root Medicago truncatula cDNA clone
NF010C04RT 5', mRNA sequence
Length = 522
Score = 74.8 bits (157), Expect(2) = 3e-021
Identities = 27/75 (36%), Positives = 43/75 (57%)
Frame = +2 / +2
Query: 164 LRRAGIIDIQFARVPCEFPGLKVGFHVEEGSTQVYFAVLVEYENGDGDVVQVDLMEKRAR 343
LR G I + + R C + G + F V EGST + ++LVE+E+GDGD+ + + E +
Sbjct: 119 LRNRGEIPVLYRRTTCVYAGKNLAFQVNEGSTPYWLSLLVEFEDGDGDISSMYIQEAGSS 298
Query: 344 RWTPMSQSWGSIWRL 388
W M+ WG+ W +
Sbjct: 299 EWLQMNHLWGANWNI 343
Score = 47.8 bits (98), Expect(2) = 3e-021
Identities = 16/40 (40%), Positives = 27/40 (67%)
Frame = +2 / +2
Query: 404 LQPPFSIRTRTDSGKTLVARDVIPLNWRPNTFYRSIVQYS 523
L+ PFS++ T +GK++ +DVIP NW P + Y S + ++
Sbjct: 356 LRGPFSVKLSTSTGKSITVKDVIPSNWSPKSTYTSRLNFT 475
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 329,015,930
Number of Sequences: 392609
Number of extensions: 3277433
Number of successful extensions: 25661
Number of sequences better than 1.0e-020: 10
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 19158603640
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)