TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2950080.2.1
(687 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF006460.1|BF006460 EST434958 DSLC Medicago truncatula c... 286 2e-076
gb|BG456115.1|BG456115 NF074G06PL1F1050 Phosphate starved l... 286 2e-076
gb|DW015943.1|DW015943 EST1224904 MTY Medicago truncatula c... 286 2e-076
gb|CX538329.1|CX538329 s13dNF80B06GS045_459910 Germinating ... 245 6e-064
gb|AC131455.5| Medicago truncatula clone mth1-42h9, complet... 110 1e-062
gb|AC124972.41| Medicago truncatula clone mth2-24j4, comple... 110 2e-062
gb|BF518587.1|BF518587 EST456035 DSIL Medicago truncatula c... 214 2e-054
gb|AW696251.2|AW696251 NF104E12ST1F1098 Developing stem Med... 200 2e-050
gb|AL381309.1|AL381309 MtBC019B11F1 MtBC Medicago truncatul... 199 5e-050
gb|AQ580067.1|AQ580067 T135621b shotgun sub-library of BAC ... 102 7e-021
>gb|BF006460.1|BF006460 EST434958 DSLC Medicago truncatula cDNA clone pDSLC-41N5, mRNA
sequence
Length = 582
Score = 286 bits (619), Expect = 2e-076
Identities = 109/167 (65%), Positives = 135/167 (80%)
Frame = +2 / +3
Query: 32 RHARPNVLVXXXXXXXXXXXCSLLADAAGLRHVNIGDLVREKSLHDGWDADLECHVINED 211
+ +PN+LV + LA+A L H+NIGDLV+EK+LHDGWD +L+ +++NED
Sbjct: 78 KRRKPNILVTGTPGTGKTTTSTALAEATQLNHINIGDLVKEKNLHDGWDDELDSYILNED 257
Query: 212 LVCDELEDMMEEGGILVDYHGCDFFPERWFDLVVVLQTDNSILHDRLTGRGYMGSKLANN 391
LVCDELED+M+EGG +VDYHGCDFFPERWFD VVVLQTDN+IL+DRL+ RGY SKL+NN
Sbjct: 258 LVCDELEDVMDEGGNIVDYHGCDFFPERWFDCVVVLQTDNTILYDRLSRRGYKESKLSNN 437
Query: 392 IECEIFQVLLEEARESYREDIVMPLRSDNVEDISRNVGALTDWVNNW 532
+ECEIFQVLLEEA+ESY ED V+ L+SD +EDISRNV LTDW+ NW
Sbjct: 438 VECEIFQVLLEEAKESYAEDKVVALKSDTIEDISRNVATLTDWIRNW 578
Score = 100 bits (213), Expect(2) = 4e-026
Identities = 38/117 (32%), Positives = 76/117 (64%)
Frame = -3 / -2
Query: 532 PIVYPVCQCTYVPTNILHVVTSQWHHDVLSVALPRLLEEHLEDFALYVVGELGTHVTSAR 353
PI P+CQ + TN+ + + Q ++ VL + LL+++L+DF +VG L V S+
Sbjct: 578 PIPDPICQSCNISTNVFNSIAFQCNNFVLRITFFSLLKQNLKDFTFDIVG*L*FFVPSSA 399
Query: 352 QAIMQN*VVSLEHNYEVKPTFWKEITSVVIY*YTTFFHHVLKLVAHQVLVDDMALEV 182
Q ++QN V+SL++N+ +KP+ KEIT+++++ + H++LK + HQ+ + ++ +++
Sbjct: 398 QTVIQNGVISLKYNHTIKPSLRKEITAMIVHNIPSLIHNILKFITHQIFIKNVRIKL 228
Score = 38.6 bits (78), Expect(2) = 4e-026
Identities = 17/31 (54%), Positives = 22/31 (70%)
Frame = -1 / -3
Query: 189 SRSASQPS*RLFSRTRSPMLTWRRPAASARS 97
S S+SQPS + FS T+SP+L W ASAR+
Sbjct: 235 SSSSSQPSCKFFSLTKSPILMWLS*VASARA 143
>gb|BG456115.1|BG456115 NF074G06PL1F1050 Phosphate starved leaf Medicago truncatula cDNA
clone NF074G06PL 5', mRNA sequence
Length = 663
Score = 286 bits (619), Expect = 2e-076
Identities = 109/167 (65%), Positives = 135/167 (80%)
Frame = +2 / +2
Query: 32 RHARPNVLVXXXXXXXXXXXCSLLADAAGLRHVNIGDLVREKSLHDGWDADLECHVINED 211
+ +PN+LV + LA+A L H+NIGDLV+EK+LHDGWD +L+ +++NED
Sbjct: 134 KRRKPNILVTGTPGTGKTTTSTALAEATQLNHINIGDLVKEKNLHDGWDDELDSYILNED 313
Query: 212 LVCDELEDMMEEGGILVDYHGCDFFPERWFDLVVVLQTDNSILHDRLTGRGYMGSKLANN 391
LVCDELED+M+EGG +VDYHGCDFFPERWFD VVVLQTDN+IL+DRL+ RGY SKL+NN
Sbjct: 314 LVCDELEDVMDEGGNIVDYHGCDFFPERWFDCVVVLQTDNTILYDRLSRRGYKESKLSNN 493
Query: 392 IECEIFQVLLEEARESYREDIVMPLRSDNVEDISRNVGALTDWVNNW 532
+ECEIFQVLLEEA+ESY ED V+ L+SD +EDISRNV LTDW+ NW
Sbjct: 494 VECEIFQVLLEEAKESYAEDKVVALKSDTIEDISRNVATLTDWIRNW 634
Score = 100 bits (213), Expect(2) = 4e-026
Identities = 38/117 (32%), Positives = 76/117 (64%)
Frame = -3 / -3
Query: 532 PIVYPVCQCTYVPTNILHVVTSQWHHDVLSVALPRLLEEHLEDFALYVVGELGTHVTSAR 353
PI P+CQ + TN+ + + Q ++ VL + LL+++L+DF +VG L V S+
Sbjct: 634 PIPDPICQSCNISTNVFNSIAFQCNNFVLRITFFSLLKQNLKDFTFDIVG*L*FFVPSSA 455
Query: 352 QAIMQN*VVSLEHNYEVKPTFWKEITSVVIY*YTTFFHHVLKLVAHQVLVDDMALEV 182
Q ++QN V+SL++N+ +KP+ KEIT+++++ + H++LK + HQ+ + ++ +++
Sbjct: 454 QTVIQNGVISLKYNHTIKPSLRKEITAMIVHNIPSLIHNILKFITHQIFIKNVRIKL 284
Score = 38.6 bits (78), Expect(2) = 4e-026
Identities = 17/31 (54%), Positives = 22/31 (70%)
Frame = -1 / -1
Query: 189 SRSASQPS*RLFSRTRSPMLTWRRPAASARS 97
S S+SQPS + FS T+SP+L W ASAR+
Sbjct: 291 SSSSSQPSCKFFSLTKSPILMWLS*VASARA 199
>gb|DW015943.1|DW015943 EST1224904 MTY Medicago truncatula cDNA clone MTYAB47, mRNA
sequence
Length = 839
Score = 286 bits (619), Expect = 2e-076
Identities = 109/167 (65%), Positives = 135/167 (80%)
Frame = +2 / +2
Query: 32 RHARPNVLVXXXXXXXXXXXCSLLADAAGLRHVNIGDLVREKSLHDGWDADLECHVINED 211
+ +PN+LV + LA+A L H+NIGDLV+EK+LHDGWD +L+ +++NED
Sbjct: 176 KRRKPNILVTGTPGTGKTTTSTALAEATQLNHINIGDLVKEKNLHDGWDDELDSYILNED 355
Query: 212 LVCDELEDMMEEGGILVDYHGCDFFPERWFDLVVVLQTDNSILHDRLTGRGYMGSKLANN 391
LVCDELED+M+EGG +VDYHGCDFFPERWFD VVVLQTDN+IL+DRL+ RGY SKL+NN
Sbjct: 356 LVCDELEDVMDEGGNIVDYHGCDFFPERWFDCVVVLQTDNTILYDRLSRRGYKESKLSNN 535
Query: 392 IECEIFQVLLEEARESYREDIVMPLRSDNVEDISRNVGALTDWVNNW 532
+ECEIFQVLLEEA+ESY ED V+ L+SD +EDISRNV LTDW+ NW
Sbjct: 536 VECEIFQVLLEEAKESYAEDKVVALKSDTIEDISRNVATLTDWIRNW 676
Score = 100 bits (213), Expect(2) = 4e-026
Identities = 38/117 (32%), Positives = 76/117 (64%)
Frame = -3 / -2
Query: 532 PIVYPVCQCTYVPTNILHVVTSQWHHDVLSVALPRLLEEHLEDFALYVVGELGTHVTSAR 353
PI P+CQ + TN+ + + Q ++ VL + LL+++L+DF +VG L V S+
Sbjct: 676 PIPDPICQSCNISTNVFNSIAFQCNNFVLRITFFSLLKQNLKDFTFDIVG*L*FFVPSSA 497
Query: 352 QAIMQN*VVSLEHNYEVKPTFWKEITSVVIY*YTTFFHHVLKLVAHQVLVDDMALEV 182
Q ++QN V+SL++N+ +KP+ KEIT+++++ + H++LK + HQ+ + ++ +++
Sbjct: 496 QTVIQNGVISLKYNHTIKPSLRKEITAMIVHNIPSLIHNILKFITHQIFIKNVRIKL 326
Score = 38.6 bits (78), Expect(2) = 4e-026
Identities = 17/31 (54%), Positives = 22/31 (70%)
Frame = -1 / -3
Query: 189 SRSASQPS*RLFSRTRSPMLTWRRPAASARS 97
S S+SQPS + FS T+SP+L W ASAR+
Sbjct: 333 SSSSSQPSCKFFSLTKSPILMWLS*VASARA 241
>gb|CX538329.1|CX538329 s13dNF80B06GS045_459910 Germinating Seed Medicago truncatula cDNA,
mRNA sequence
Length = 627
Score = 245 bits (529), Expect = 6e-064
Identities = 95/150 (63%), Positives = 119/150 (79%)
Frame = +2 / +2
Query: 32 RHARPNVLVXXXXXXXXXXXCSLLADAAGLRHVNIGDLVREKSLHDGWDADLECHVINED 211
+ +PN+LV + LA+A L H+NIGDLV+EK+LHDGWD +L+ +++NED
Sbjct: 176 KRRKPNILVTGTPGTGKTTTSTALAEATQLNHINIGDLVKEKNLHDGWDDELDSYILNED 355
Query: 212 LVCDELEDMMEEGGILVDYHGCDFFPERWFDLVVVLQTDNSILHDRLTGRGYMGSKLANN 391
LVCDELED+M+EGG +VDYHGCDFFPERWFD VVVLQTDN+IL+DRL+ RGY SKL+NN
Sbjct: 356 LVCDELEDVMDEGGNIVDYHGCDFFPERWFDCVVVLQTDNTILYDRLSRRGYKESKLSNN 535
Query: 392 IECEIFQVLLEEARESYREDIVMPLRSDNV 481
+ECEIFQVLLEEA+ESY E V+ L SD +
Sbjct: 536 VECEIFQVLLEEAKESYAEGQVVALXSDTI 625
>gb|AC131455.5| Medicago truncatula clone mth1-42h9, complete sequence
Length = 96063
Score = 110 bits (234), Expect(3) = 1e-062
Identities = 43/61 (70%), Positives = 50/61 (81%)
Frame = +2 / +3
Query: 350 LTGRGYMGSKLANNIECEIFQVLLEEARESYREDIVMPLRSDNVEDISRNVGALTDWVNN 529
L RGY SKL+NN+ECEIFQVLLEEA+ESY ED V+ L+SD +EDISRNV LTDW+ N
Sbjct: 95085 LCDRGYKESKLSNNVECEIFQVLLEEAKESYAEDKVVALKSDTIEDISRNVATLTDWIRN 95264
Query: 530 W 532
W
Sbjct: 95265 W 95267
Score = 107 bits (228), Expect(3) = 1e-062
Identities = 40/49 (81%), Positives = 46/49 (93%)
Frame = +2 / +3
Query: 215 VCDELEDMMEEGGILVDYHGCDFFPERWFDLVVVLQTDNSILHDRLTGR 361
VCDELED+M+EGG +VDYHGCDFFPERWFD VVVLQTDN+IL+DRL+ R
Sbjct: 94854 VCDELEDVMDEGGNIVDYHGCDFFPERWFDCVVVLQTDNTILYDRLSRR 95000
Score = 79.9 bits (168), Expect(3) = 1e-062
Identities = 29/62 (46%), Positives = 42/62 (67%)
Frame = +2 / +3
Query: 32 RHARPNVLVXXXXXXXXXXXCSLLADAAGLRHVNIGDLVREKSLHDGWDADLECHVINED 211
+ +PN+LV + LA+A L H+NIGDLV+EK+LHDGWD +L+ +++NED
Sbjct: 94347 KRRKPNILVTGTPGTGKTTTSTALAEATQLNHINIGDLVKEKNLHDGWDDELDSYILNED 94526
Query: 212 LV 217
LV
Sbjct: 94527 LV 94532
>gb|AC124972.41| Medicago truncatula clone mth2-24j4, complete sequence
Length = 124069
Score = 110 bits (234), Expect(3) = 2e-062
Identities = 43/61 (70%), Positives = 50/61 (81%)
Frame = +2 / -2
Query: 350 LTGRGYMGSKLANNIECEIFQVLLEEARESYREDIVMPLRSDNVEDISRNVGALTDWVNN 529
L RGY SKL+NN+ECEIFQVLLEEA+ESY ED V+ L+SD +EDISRNV LTDW+ N
Sbjct: 58791 LCDRGYKESKLSNNVECEIFQVLLEEAKESYAEDKVVALKSDTIEDISRNVATLTDWIRN 58612
Query: 530 W 532
W
Sbjct: 58611 W 58609
Score = 107 bits (228), Expect(3) = 2e-062
Identities = 40/49 (81%), Positives = 46/49 (93%)
Frame = +2 / -2
Query: 215 VCDELEDMMEEGGILVDYHGCDFFPERWFDLVVVLQTDNSILHDRLTGR 361
VCDELED+M+EGG +VDYHGCDFFPERWFD VVVLQTDN+IL+DRL+ R
Sbjct: 59022 VCDELEDVMDEGGNIVDYHGCDFFPERWFDCVVVLQTDNTILYDRLSRR 58876
Score = 79.9 bits (168), Expect(3) = 2e-062
Identities = 29/62 (46%), Positives = 42/62 (67%)
Frame = +2 / -2
Query: 32 RHARPNVLVXXXXXXXXXXXCSLLADAAGLRHVNIGDLVREKSLHDGWDADLECHVINED 211
+ +PN+LV + LA+A L H+NIGDLV+EK+LHDGWD +L+ +++NED
Sbjct: 59529 KRRKPNILVTGTPGTGKTTTSTALAEATQLNHINIGDLVKEKNLHDGWDDELDSYILNED 59350
Query: 212 LV 217
LV
Sbjct: 59349 LV 59344
>gb|BF518587.1|BF518587 EST456035 DSIL Medicago truncatula cDNA clone pDSIL-5L1, mRNA
sequence
Length = 325
Score = 214 bits (461), Expect = 2e-054
Identities = 83/108 (76%), Positives = 97/108 (89%)
Frame = +2 / +2
Query: 200 INEDLVCDELEDMMEEGGILVDYHGCDFFPERWFDLVVVLQTDNSILHDRLTGRGYMGSK 379
+NEDLVCDELED+M+EGG +VDYHGCDFFPERWFD VVVLQTDN+IL+DRL+ RGY SK
Sbjct: 2 LNEDLVCDELEDVMDEGGNIVDYHGCDFFPERWFDCVVVLQTDNTILYDRLSRRGYKESK 181
Query: 380 LANNIECEIFQVLLEEARESYREDIVMPLRSDNVEDISRNVGALTDWV 523
L+NN+ECEIFQVLLEEA+ESY ED V+ L+SD +EDISRNV LTDW+
Sbjct: 182 LSNNVECEIFQVLLEEAKESYAEDKVVALKSDTIEDISRNVATLTDWI 325
>gb|AW696251.2|AW696251 NF104E12ST1F1098 Developing stem Medicago truncatula cDNA clone
NF104E12ST 5', mRNA sequence
Length = 617
Score = 200 bits (431), Expect = 2e-050
Identities = 75/121 (61%), Positives = 96/121 (79%)
Frame = +2 / +2
Query: 32 RHARPNVLVXXXXXXXXXXXCSLLADAAGLRHVNIGDLVREKSLHDGWDADLECHVINED 211
+ +PN+LV + LA+A L H+NIGDLV+EK+LHDGWD +L+ +++NED
Sbjct: 233 KRRKPNILVTGTPGTGKTTTSTALAEATQLNHINIGDLVKEKNLHDGWDDELDSYILNED 412
Query: 212 LVCDELEDMMEEGGILVDYHGCDFFPERWFDLVVVLQTDNSILHDRLTGRGYMGSKLANN 391
LVCDELED+M+EGG +VDYHGCDFFPERWFD VVVLQTDN+IL+DRL+ RGY SKL+NN
Sbjct: 413 LVCDELEDVMDEGGNIVDYHGCDFFPERWFDCVVVLQTDNTILYDRLSRRGYKESKLSNN 592
Query: 392 I 394
+
Sbjct: 593 V 595
>gb|AL381309.1|AL381309 MtBC019B11F1 MtBC Medicago truncatula cDNA clone MtBC019B1 T3, mRNA
sequence
Length = 489
Score = 199 bits (428), Expect = 5e-050
Identities = 75/120 (62%), Positives = 95/120 (79%)
Frame = +2 / +3
Query: 32 RHARPNVLVXXXXXXXXXXXCSLLADAAGLRHVNIGDLVREKSLHDGWDADLECHVINED 211
+ +PN+LV + LA+A L H+NIGDLV+EK+LHDGWD +L+ +++NED
Sbjct: 129 KRRKPNILVTGTPGTGKTTTSTALAEATQLNHINIGDLVKEKNLHDGWDDELDSYILNED 308
Query: 212 LVCDELEDMMEEGGILVDYHGCDFFPERWFDLVVVLQTDNSILHDRLTGRGYMGSKLANN 391
LVCDELED+M+EGG +VDYHGCDFFPERWFD VVVLQTDN+IL+DRL+ RGY SKL+NN
Sbjct: 309 LVCDELEDVMDEGGNIVDYHGCDFFPERWFDCVVVLQTDNTILYDRLSRRGYKESKLSNN 488
>gb|AQ580067.1|AQ580067 T135621b shotgun sub-library of BAC clone 42H09 Medicago truncatula
genomic clone 42-H-09-C-045, DNA sequence
Length = 532
Score = 102 bits (217), Expect = 7e-021
Identities = 39/53 (73%), Positives = 46/53 (86%)
Frame = +2 / -3
Query: 374 SKLANNIECEIFQVLLEEARESYREDIVMPLRSDNVEDISRNVGALTDWVNNW 532
SKL+NN+ECEIFQVLLEEA+ESY ED V+ L+SD +EDISRNV LTDW+ NW
Sbjct: 527 SKLSNNVECEIFQVLLEEAKESYAEDKVVALKSDTIEDISRNVATLTDWIRNW 369
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 522,037,021
Number of Sequences: 392609
Number of extensions: 6210489
Number of successful extensions: 34973
Number of sequences better than 1.0e-020: 11
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 21931559430
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)