TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2750698.2.1
(1126 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AC152185.1| Medicago truncatula chromosome 2 BAC clone m... 144 e-115
gb|BF003718.1|BF003718 EST432216 KV1 Medicago truncatula cD... 410 e-113
gb|CG967115.1|CG967115 MBEEC59TFC mth2 Medicago truncatula ... 144 3e-038
gb|CA920838.1|CA920838 EST638556 MTUS Medicago truncatula c... 135 1e-030
gb|CG956804.1|CG956804 MBEED59TFC mth2 Medicago truncatula ... 134 3e-030
gb|BG649060.1|BG649060 EST510679 HOGA Medicago truncatula c... 82 1e-028
>gb|AC152185.1| Medicago truncatula chromosome 2 BAC clone mth2-97e5, complete sequence
Length = 132467
Score = 144 bits (310), Expect(5) = e-115
Identities = 56/72 (77%), Positives = 66/72 (91%)
Frame = +1 / -3
Query: 127 GPPLSGRYDMGAICMIEHSEKLQNLVNDALDKGAEIAVRGSFGNLGEDAVDQFFPPTGLV 306
GPPL+G+YDMGA+CM EHSEKL LVNDALDKGAEI RGSFG++GEDAVDQ+FPPT +V
Sbjct: 90543 GPPLAGKYDMGALCMHEHSEKLLGLVNDALDKGAEIVARGSFGHIGEDAVDQYFPPTVIV 90364
Query: 307 NVDHTMKIMQEE 342
NV+H+MK+MQEE
Sbjct: 90363 NVNHSMKLMQEE 90328
Score = 128 bits (275), Expect(5) = e-115
Identities = 51/63 (80%), Positives = 56/63 (88%)
Frame = +1 / -2
Query: 346 FGPIIPIMKFSSDEEAIKLANDSKYGLGCAVFSGNQKRAIRIASQLHCGVAAINDFASSY 525
FGPI+PIMKFSSDEE IKLANDSKYGL CAVFSG+Q A IAS +HCGVAA+NDFAS+Y
Sbjct: 90175 FGPIMPIMKFSSDEEVIKLANDSKYGLSCAVFSGSQSHAREIASHIHCGVAAVNDFASNY 89996
Query: 526 MCQ 534
MCQ
Sbjct: 89995 MCQ 89987
Score = 109 bits (232), Expect(5) = e-115
Identities = 43/49 (87%), Positives = 45/49 (91%)
Frame = +1 / -3
Query: 532 QSLPFGGVKDSGFGRFAGVEGLRACCLVKSVVEDRLWPYIRTVIPKPIQ 678
QSLPFGGVK SGFGRF GVEGLRACCLVKSVVEDR WP+I+T IPKPIQ
Sbjct: 89601 QSLPFGGVKHSGFGRFGGVEGLRACCLVKSVVEDRWWPFIKTKIPKPIQ 89455
Score = 101 bits (216), Expect(5) = 3e-058
Identities = 50/78 (64%), Positives = 54/78 (69%)
Frame = -3 / +2
Query: 341 SSCIIFIV*STFTRPVGGKN*STASSPRLPKLPLTAISAPLSRASLTRF*SFSECSIIQI 162
SSCI FI TFT VGGK *STASSP PKLP ISAPLS+AS TR SFSECS +
Sbjct: 90329 SSCINFIEWFTFTITVGGKY*STASSPICPKLPRATISAPLSKASFTRPRSFSECSCMHK 90508
Query: 161 APMSYLPDNGGPTLIDLT 108
AP+SY P +GGP LT
Sbjct: 90509 APISYFPASGGPCKKSLT 90562
Score = 98.7 bits (209), Expect(5) = 3e-058
Identities = 45/62 (72%), Positives = 50/62 (80%)
Frame = -3 / +3
Query: 530 HM*LEAKSLIAATPQCNWDAILMARFWLPEKTAQPRPYFESFASLIASSSELNFMIGIIG 351
HM* +AKSL AATPQC +AI +A LPEKTAQ PYFES ASLI SSSELNF+IG+IG
Sbjct: 89991 HM*FDAKSLTAATPQCI*EAISLA*L*LPEKTAQLSPYFESLASLITSSSELNFIIGMIG 90170
Query: 350 PN 345
PN
Sbjct: 90171 PN 90176
Score = 86.3 bits (182), Expect(5) = 6e-029
Identities = 33/62 (53%), Positives = 44/62 (70%)
Frame = +3 / -1
Query: 345 IWTYYTNHEIQF**RGYQTCKRLKIWSWLCCFFGQPEACHQNSVPVTLWGSSNQ*FCFKL 524
IWTY+ ++E+ **RGYQ C LKIW+ LCCF Q ++C ++S TLWG + *FC +L
Sbjct: 90176 IWTYHADNEV*L**RGYQAC**LKIWAQLCCFLRQSKSCQRDSFSYTLWGRCS**FCIEL 89997
Query: 525 HV 530
HV
Sbjct: 89996 HV 89991
Score = 80.8 bits (170), Expect(5) = e-115
Identities = 30/42 (71%), Positives = 39/42 (92%)
Frame = +1 / -3
Query: 679 YPVSEHGFEFQQLLVETLYGYSVWDRLRSLVNLIKMVTEQNS 804
YPV+E+GFEFQ+ LVE LYG S+WDRL++LVN++KM+TEQNS
Sbjct: 89370 YPVAENGFEFQESLVEALYGLSIWDRLQALVNVLKMLTEQNS 89245
Score = 72.1 bits (151), Expect(4) = 2e-023
Identities = 37/64 (57%), Positives = 41/64 (64%)
Frame = -1 / +1
Query: 529 TCNLKQNH*LLLPHSVTGTLF*WHASGCPKKQHSQDHILSRLQV**PLHQN*IS*LV**V 350
TCN QNH*L PH+V L WH C +KQHS HILS Q **PLHQ+* S **V
Sbjct: 89992 TCNSMQNH*LQRPHNVYEKLSLWHDFDCLRKQHS*AHILSH*QA**PLHQS*TSLSA**V 90171
Query: 349 QMSL 338
QM +
Sbjct: 90172 QMPI 90183
Score = 72.1 bits (151), Expect(4) = 2e-031
Identities = 33/65 (50%), Positives = 43/65 (66%)
Frame = -2 / +2
Query: 534 LAHVT*SKIIDCCYPTV*LGRYSDGTLLVARKNSTAKTIF*VVCKFDSLFIRTEFHDWYN 355
L HV KII+C PT+ + Y G L A +NSTA+ IF*V+ K D+LFIR + H ++
Sbjct: 89987 LTHVIRCKIINCSDPTMYMRSYLSGMTLTA*ENSTAEPIF*VISKLDNLFIRAKLHYRHD 90166
Query: 354 RSKCL 340
RSKCL
Sbjct: 90167 RSKCL 90181
Score = 67.0 bits (140), Expect(5) = e-115
Identities = 25/38 (65%), Positives = 32/38 (84%)
Frame = +1 / -3
Query: 10 AALQSSGQNCAGAERFYVHDDIYSAFVSQIVKTVKSIS 123
A LQS GQNCAGAERFYVH +IY +FVS++ K +KS++
Sbjct: 91473 AVLQSGGQNCAGAERFYVHRNIYPSFVSKVTKIIKSVT 91360
Score = 63.4 bits (132), Expect(4) = 2e-031
Identities = 32/72 (44%), Positives = 41/72 (56%)
Frame = -2 / +1
Query: 342 LFLHNFHCVINIHQASWWEKLINCIFPKIAKTPPDGNFGTFV*SIINKILKFLRVLNHTD 163
LFLH FH V++IH + LIN IF ++KT NF +FV*SII K K R+ H
Sbjct: 90328 LFLHQFH*VVHIHNHRRGKILINSIFAYMSKTSTCNNFSSFV*SIIYKT*KLFRMFVHA* 90507
Query: 162 CTHVISA**WGA 127
+H+I W A
Sbjct: 90508 SSHIILPSKWWA 90543
Score = 51.9 bits (107), Expect(5) = 3e-058
Identities = 21/27 (77%), Positives = 24/27 (88%)
Frame = -3 / +2
Query: 674 IGFGITVLIYGHNLSSTTDFTRQQARK 594
IGFGI VL+ GH+LSSTTDFTRQ AR+
Sbjct: 89459 IGFGILVLMNGHHLSSTTDFTRQHARR 89539
Score = 50.6 bits (104), Expect(4) = 2e-031
Identities = 22/40 (55%), Positives = 27/40 (67%)
Frame = -2 / +1
Query: 651 NIWPQSIFYNRLHKAASSQAFYASKSPESAVFNTTEWQRL 532
N WP SIF L+KAA SQAF ++S +S VF +WQRL
Sbjct: 89482 NEWPPSIFNY*LYKAACSQAFNTTESSKSTVFYAPKWQRL 89601
Score = 50.1 bits (103), Expect(5) = 3e-058
Identities = 23/40 (57%), Positives = 25/40 (62%)
Frame = -3 / +2
Query: 797 CSVTIFIRFTRDRNLSHTL*PYRVSTSNCWNSNPCSETGY 678
CSV+IF FT SH L PY STS+ NSNP S TGY
Sbjct: 89252 CSVSIFKTFTNACKRSHMLRPYSASTSDS*NSNPFSATGY 89371
Score = 46.0 bits (94), Expect(4) = 2e-023
Identities = 26/66 (39%), Positives = 33/66 (50%)
Frame = -1 / +3
Query: 346 MSLLA*FSLCDQHSPGQLVGKIDQLHLPQDCQNSP*RQFRHLCLEHH*QDSEVSQSAQSY 167
+ LLA SL HS DQ HL QN +QF+ LCL+HH QD E Q+ ++
Sbjct: 90324 LPLLASISLSGSHSQSP*GENTDQQHLRLYVQNFHVQQFQLLCLKHHLQDLEAFQNVRAC 90503
Query: 166 RLHPCH 149
P H
Sbjct: 90504 IKLPYH 90521
Score = 42.3 bits (86), Expect(5) = 6e-029
Identities = 17/37 (45%), Positives = 22/37 (59%)
Frame = +3 / -2
Query: 6 RSCSAI*WAELRWC*KILCSR*HLFCLCFTDSEDSQI 116
+ CS++ WAEL WC* ILC HL C +D +I
Sbjct: 91477 QGCSSVRWAELCWC*TILCP*EHLSIFCQQGYQDHKI 91367
Score = 41.4 bits (84), Expect(4) = 2e-023
Identities = 20/39 (51%), Positives = 25/39 (64%)
Frame = -1 / +3
Query: 646 MATIYLLQQTSQGSKLASLLRQQISRIRCL*HHRMAKTG 530
MATIYL T QGS LA L +I +I C+ +MA+TG
Sbjct: 89487 MATIYLQLLTLQGSMLAGLQHHRIVQIHCVLRPQMAETG 89603
Score = 41.4 bits (84), Expect(5) = 6e-029
Identities = 31/72 (43%), Positives = 36/72 (50%)
Frame = +2 / -1
Query: 128 APHYQADMTWVQSV*LSTLRNFRILLMML*TKVPKLPSGGVLAILGKMQLINFSHQLAW* 307
A H M W + L++F +L MML*TK KL VL I KM LI+ *
Sbjct: 90542 AHHLLGSMIWELYACTNILKSF*VL*MML*TKELKLLHVEVLDI*AKMLLISIFPLR*L* 90363
Query: 308 MLITQ*KLCKKR 343
M TQ* CKKR
Sbjct: 90362 M*TTQ*N*CKKR 90327
Score = 35.4 bits (71), Expect(5) = 3e-058
Identities = 19/36 (52%), Positives = 20/36 (55%)
Frame = -3 / +2
Query: 116 DLTVFTICETKAE*MSS*T*NLSAPAQFCPLDCRAA 9
D + TK *M T*N SAPAQFCP D R A
Sbjct: 91367 DFMILVTLLTKDG*MFLWT*NRSAPAQFCPPD*RTA 91474
Score = 35.4 bits (71), Expect(4) = 2e-031
Identities = 19/36 (52%), Positives = 26/36 (72%)
Frame = -2 / +1
Query: 117 RFDCLHYL*NKGRIDVIVNIKSFSTSAILPTRLQSS 10
RF L L +K I+V ++IKSFSTS +LPT L++S
Sbjct: 91366 RFYDLGNLADKRWINVPMDIKSFSTSTVLPT*LKNS 91473
Score = 34.5 bits (69), Expect(4) = 2e-023
Identities = 13/26 (50%), Positives = 17/26 (65%)
Frame = -1 / +3
Query: 88 QRQNRCHREHKIFQHQRNSAH*IAEQ 11
Q+ ++C HKI QHQ +SAH EQ
Sbjct: 91395 QKMDKCSYGHKIVQHQHSSAHLTEEQ 91472
Score = 34.5 bits (69), Expect(5) = 6e-029
Identities = 15/26 (57%), Positives = 21/26 (80%)
Frame = +3 / -2
Query: 597 ASLLPCEVCCRR*IVAIY*NSDPEAY 674
AS+LPC+V R*+VAI+*+ D +AY
Sbjct: 89536 ASMLPCKVSS*R*MVAIH*DQDTKAY 89459
Score = 32.7 bits (65), Expect(5) = 6e-029
Identities = 15/41 (36%), Positives = 23/41 (56%)
Frame = +3 / -2
Query: 678 VSRLRAWI*VPAVACGDSVWL*RVGQVAVPCESYKDGYRAE 800
VS R WI*+ V C ++W * +G A + +++ Y AE
Sbjct: 89371 VSCGRKWI*ISGVTC*SAIWS*HMGSFASIGKRFENAY*AE 89249
>gb|BF003718.1|BF003718 EST432216 KV1 Medicago truncatula cDNA clone pKV1-13B5, mRNA
sequence
Length = 624
Score = 410 bits (890), Expect = e-113
Identities = 164/208 (78%), Positives = 187/208 (89%)
Frame = +1 / +1
Query: 169 MIEHSEKLQNLVNDALDKGAEIAVRGSFGNLGEDAVDQFFPPTGLVNVDHTMKIMQEETF 348
M EHSEKL LVNDALDKGAEI RGSFG++GEDAVDQ+FPPT +VNV+H+MK+MQEE F
Sbjct: 1 MHEHSEKLLGLVNDALDKGAEIVARGSFGHIGEDAVDQYFPPTVIVNVNHSMKLMQEEAF 180
Query: 349 GPIIPIMKFSSDEEAIKLANDSKYGLGCAVFSGNQKRAIRIASQLHCGVAAINDFASSYM 528
GPI+PIMKFSSDEE IKLANDSKYGL CAVFSG+Q A IAS +HCGVAA+NDFAS+YM
Sbjct: 181 GPIMPIMKFSSDEEVIKLANDSKYGLSCAVFSGSQSHAREIASHIHCGVAAVNDFASNYM 360
Query: 529 CQSLPFGGVKDSGFGRFAGVEGLRACCLVKSVVEDRLWPYIRTVIPKPIQYPVSEHGFEF 708
CQSLPFGGVK SGFGRF GVEGLRACCLVKSVVEDR WP+I+T IPKPIQYPV+E+GFEF
Sbjct: 361 CQSLPFGGVKHSGFGRFGGVEGLRACCLVKSVVEDRWWPFIKTKIPKPIQYPVAENGFEF 540
Query: 709 QQLLVETLYGYSVWDRLRSLVNLIKMVT 792
Q+ LVE LYG S+WDRL++LVN++KM+T
Sbjct: 541 QESLVEALYGLSIWDRLQALVNVLKMLT 624
Score = 189 bits (407), Expect = 8e-047
Identities = 95/206 (46%), Positives = 126/206 (61%)
Frame = -2 / -1
Query: 786 HLYKIHKGPQPVPHAIAIQSLHKQLLELKSML*DGILDRLRDHCSNIWPQSIFYNRLHKA 607
H +++ Q +P+A I +K LL+ KS+ IL+RL N WP SIF L+KA
Sbjct: 618 HFQNVYQCLQTIPYAKTI*RFNK*LLKFKSIFCHRILNRLWYLGLNEWPPSIFNY*LYKA 439
Query: 606 ASSQAFYASKSPESAVFNTTEWQRLAHVT*SKIIDCCYPTV*LGRYSDGTLLVARKNSTA 427
A SQAF ++S +S VF +WQRL HV KII+C PT+ + Y G L A +NSTA
Sbjct: 438 ACSQAFNTTESSKSTVFYAPKWQRLTHVIRCKIINCSDPTMYMRSYLSGMTLTA*ENSTA 259
Query: 426 KTIF*VVCKFDSLFIRTEFHDWYNRSKCLFLHNFHCVINIHQASWWEKLINCIFPKIAKT 247
+ IF*V+ K D+LFIR + H ++RSKCLFLH FH V++IH + LIN IF ++KT
Sbjct: 258 EPIF*VISKLDNLFIRAKLHYRHDRSKCLFLHQFH*VVHIHNHRRGKILINSIFAYMSKT 79
Query: 246 PPDGNFGTFV*SIINKILKFLRVLNH 169
NF +FV*SII K K R+ H
Sbjct: 78 STCNNFSSFV*SIIYKT*KLFRMFVH 1
Score = 180 bits (387), Expect(2) = 2e-065
Identities = 87/123 (70%), Positives = 94/123 (76%)
Frame = -3 / -2
Query: 542 GKDWHM*LEAKSLIAATPQCNWDAILMARFWLPEKTAQPRPYFESFASLIASSSELNFMI 363
G+D HM* +AKSL AATPQC +AI +A LPEKTAQ PYFES ASLI SSSELNF+I
Sbjct: 374 GRD*HM*FDAKSLTAATPQCI*EAISLA*L*LPEKTAQLSPYFESLASLITSSSELNFII 195
Query: 362 GIIGPNVSSCIIFIV*STFTRPVGGKN*STASSPRLPKLPLTAISAPLSRASLTRF*SFS 183
G+IGPN SSCI FI TFT VGGK *STASSP PKLP ISAPLS+AS TR SFS
Sbjct: 194 GMIGPNASSCINFIEWFTFTITVGGKY*STASSPICPKLPRATISAPLSKASFTRPRSFS 15
Query: 182 ECS 174
ECS
Sbjct: 14 ECS 6
Score = 162 bits (349), Expect = 8e-039
Identities = 93/189 (49%), Positives = 108/189 (57%)
Frame = -1 / -3
Query: 736 HTESPQATAGTQIHALRRDTG*ASGSLF*YMATIYLLQQTSQGSKLASLLRQQISRIRCL 557
H Q T QIH L +DT *A S * MATIYL T QGS LA L +I +I C+
Sbjct: 568 HIALQQVTPEIQIHFLPQDTE*ALVSWS**MATIYLQLLTLQGSMLAGLQHHRIVQIHCV 389
Query: 556 *HHRMAKTGTCNLKQNH*LLLPHSVTGTLF*WHASGCPKKQHSQDHILSRLQV**PLHQN 377
+MA+T TCN QNH*L PH+V L WH C +KQHS HILS Q **PLHQ+
Sbjct: 388 LRPQMAETDTCNSMQNH*LQRPHNVYEKLSLWHDFDCLRKQHS*AHILSH*QA**PLHQS 209
Query: 376 *IS*LV**VQMSLLA*FSLCDQHSPGQLVGKIDQLHLPQDCQNSP*RQFRHLCLEHH*QD 197
* S **VQM LLA SL HS DQ HL QN +QF+ LCL+HH QD
Sbjct: 208 *TSLSA**VQMPLLASISLSGSHSQSP*GENTDQQHLRLYVQNFHVQQFQLLCLKHHLQD 29
Query: 196 SEVSQSAQS 170
E Q+ ++
Sbjct: 28 LEAFQNVRA 2
Score = 111 bits (238), Expect(2) = 5e-036
Identities = 49/99 (49%), Positives = 62/99 (62%)
Frame = +3 / +3
Query: 246 EFWQSWGRCS*SIFPTNWPGEC*SHNENYARRDIWTYYTNHEIQF**RGYQTCKRLKIWS 425
+FW RC *S+F EC NE ARR IWTY+ ++E+ **RGYQ C LKIW+
Sbjct: 78 KFWTYRRRCC*SVFSPYGDCECEPLNEIDARRGIWTYHADNEV*L**RGYQAC**LKIWA 257
Query: 426 WLCCFFGQPEACHQNSVPVTLWGSSNQ*FCFKLHVPVFA 542
LCCF Q ++C ++S TLWG + *FC +LHV V A
Sbjct: 258 QLCCFLRQSKSCQRDSFSYTLWGRCS**FCIELHVSVSA 374
Score = 106 bits (227), Expect = 5e-022
Identities = 66/132 (50%), Positives = 78/132 (59%)
Frame = +2 / +2
Query: 182 LRNFRILLMML*TKVPKLPSGGVLAILGKMQLINFSHQLAW*MLITQ*KLCKKRHLDLLY 361
L++F +L MML*TK KL VL I KM LI+ *M TQ* CKKRHLDL
Sbjct: 14 LKSF*VL*MML*TKELKLLHVEVLDI*AKMLLISIFPLR*L*M*TTQ*N*CKKRHLDLSC 193
Query: 362 QS*NSVLMKRLSNLQTTQNMVLAVLFFRATRSVPSE*RPSYTVG*QQSMILLQVTCASLC 541
+ *+ LMKRLS+L TQNM AVLF +A + +P * Y VG Q MIL ++TC SLC
Sbjct: 194 R**SLALMKRLSSLLMTQNMGSAVLFSQAVKVMPER*LLIYIVGSLQLMILHRITCVSLC 373
Query: 542 HSVVLKTADSGD 577
H T D D
Sbjct: 374 HLGA*NTVDLDD 409
Score = 91.3 bits (193), Expect(2) = 2e-065
Identities = 42/66 (63%), Positives = 47/66 (71%)
Frame = -3 / -2
Query: 791 VTIFIRFTRDRNLSHTL*PYRVSTSNCWNSNPCSETGYWIGFGITVLIYGHNLSSTTDFT 612
V+IF FT SH L PY STS+ NSNP S TGY IGFGI VL+ GH+LSSTTDFT
Sbjct: 623 VSIFKTFTNACKRSHMLRPYSASTSDS*NSNPFSATGY*IGFGILVLMNGHHLSSTTDFT 444
Query: 611 RQQARK 594
RQ AR+
Sbjct: 443 RQHARR 426
Score = 61.6 bits (128), Expect(2) = 5e-036
Identities = 28/65 (43%), Positives = 42/65 (64%)
Frame = +3 / +3
Query: 597 ASLLPCEVCCRR*IVAIY*NSDPEAYPVSRLRAWI*VPAVACGDSVWL*RVGQVAVPCES 776
AS+LPC+V R*+VAI+*+ D +AY VS R WI*+ V C ++W * +G A +
Sbjct: 429 ASMLPCKVSS*R*MVAIH*DQDTKAYSVSCGRKWI*ISGVTC*SAIWS*HMGSFASIGKR 608
Query: 777 YKDGY 791
+++ Y
Sbjct: 609 FENAY 623
>gb|CG967115.1|CG967115 MBEEC59TFC mth2 Medicago truncatula genomic clone 36J21, DNA
sequence
Length = 788
Score = 144 bits (310), Expect(2) = 3e-038
Identities = 56/72 (77%), Positives = 66/72 (91%)
Frame = +1 / -2
Query: 127 GPPLSGRYDMGAICMIEHSEKLQNLVNDALDKGAEIAVRGSFGNLGEDAVDQFFPPTGLV 306
GPPL+G+YDMGA+CM EHSEKL LVNDALDKGAEI RGSFG++GEDAVDQ+FPPT +V
Sbjct: 424 GPPLAGKYDMGALCMHEHSEKLLGLVNDALDKGAEIVARGSFGHIGEDAVDQYFPPTVIV 245
Query: 307 NVDHTMKIMQEE 342
NV+H+MK+MQEE
Sbjct: 244 NVNHSMKLMQEE 209
Score = 101 bits (216), Expect(2) = 3e-025
Identities = 50/78 (64%), Positives = 54/78 (69%)
Frame = -3 / +3
Query: 341 SSCIIFIV*STFTRPVGGKN*STASSPRLPKLPLTAISAPLSRASLTRF*SFSECSIIQI 162
SSCI FI TFT VGGK *STASSP PKLP ISAPLS+AS TR SFSECS +
Sbjct: 210 SSCINFIEWFTFTITVGGKY*STASSPICPKLPRATISAPLSKASFTRPRSFSECSCMHK 389
Query: 161 APMSYLPDNGGPTLIDLT 108
AP+SY P +GGP LT
Sbjct: 390 APISYFPASGGPCKKSLT 443
Score = 39.1 bits (79), Expect(2) = 3e-038
Identities = 15/18 (83%), Positives = 16/18 (88%)
Frame = +1 / -1
Query: 346 FGPIIPIMKFSSDEEAIK 399
FGPI+PIMKFS DEE IK
Sbjct: 56 FGPIMPIMKFSCDEEVIK 3
Score = 38.2 bits (77), Expect(2) = 3e-025
Identities = 15/19 (78%), Positives = 18/19 (94%)
Frame = -3 / +1
Query: 401 SLIASSSELNFMIGIIGPN 345
SLI SSS+LNF+IG+IGPN
Sbjct: 1 SLITSSSQLNFIIGMIGPN 57
>gb|CA920838.1|CA920838 EST638556 MTUS Medicago truncatula cDNA clone MTUS-32A3, mRNA
sequence
Length = 499
Score = 135 bits (289), Expect = 1e-030
Identities = 51/68 (75%), Positives = 62/68 (91%)
Frame = +1 / -3
Query: 601 ACCLVKSVVEDRLWPYIRTVIPKPIQYPVSEHGFEFQQLLVETLYGYSVWDRLRSLVNLI 780
ACCLVKSVVEDR WP+I+T IPKPIQYP +E+GFEFQ+ LVE LYG S+WDRL++LVN++
Sbjct: 497 ACCLVKSVVEDRWWPFIKTKIPKPIQYPAAENGFEFQESLVEALYGLSIWDRLQALVNVL 318
Query: 781 KMVTEQNS 804
KM+TEQNS
Sbjct: 317 KMLTEQNS 294
>gb|CG956804.1|CG956804 MBEED59TFC mth2 Medicago truncatula genomic clone 36J22, DNA
sequence
Length = 292
Score = 134 bits (287), Expect = 3e-030
Identities = 54/78 (69%), Positives = 65/78 (83%)
Frame = +1 / -1
Query: 109 VKSISVGPPLSGRYDMGAICMIEHSEKLQNLVNDALDKGAEIAVRGSFGNLGEDAVDQFF 288
V + GPP +G+YDMGA+CM EHSEKL LVNDAL KGAEI RGSFG++GEDAVDQ+F
Sbjct: 247 VNDLLQGPPHAGKYDMGALCMHEHSEKLLGLVNDALGKGAEIVARGSFGHIGEDAVDQYF 68
Query: 289 PPTGLVNVDHTMKIMQEE 342
PPT +VNV+ +MK+MQEE
Sbjct: 67 PPTVIVNVNLSMKLMQEE 14
>gb|BG649060.1|BG649060 EST510679 HOGA Medicago truncatula cDNA clone pHOGA-24B8 5' end,
mRNA sequence
Length = 763
Score = 81.7 bits (172), Expect(3) = 1e-028
Identities = 34/82 (41%), Positives = 53/82 (64%)
Frame = +1 / +1
Query: 283 FFPPTGLVNVDHTMKIMQEETFGPIIPIMKFSSDEEAIKLANDSKYGLGCAVFSGNQKRA 462
F PT + +V +M+I +EE FGP++ + FS++EEAI LAND+ YGLG AV S + ++
Sbjct: 373 FVEPTIVTDVTTSMQIWREEVFGPVLSVKTFSTEEEAIDLANDTHYGLGSAVMSNDLEKC 552
Query: 463 IRIASQLHCGVAAINDFASSYM 528
R++ L G+ IN S++
Sbjct: 553 ERVSKALQAGIVWINCAQPSFI 618
Score = 52.4 bits (108), Expect(3) = 1e-028
Identities = 24/75 (32%), Positives = 39/75 (52%)
Frame = +1 / +1
Query: 22 SSGQNCAGAERFYVHDDIYSAFVSQIVKTVKSISVGPPLSGRYDMGAICMIEHSEKLQNL 201
++GQ C+ R VH+ I FVS++VK ++I + PL +G I +K+ N
Sbjct: 118 TNGQICSATSRLIVHESIAVEFVSKLVKWAENIKISDPLEKGCRLGPIVSEAQYKKVLNF 297
Query: 202 VNDALDKGAEIAVRG 246
++ A +GA I G
Sbjct: 298 ISTAKSEGATILTGG 342
Score = 32.2 bits (64), Expect(3) = 1e-028
Identities = 15/28 (53%), Positives = 17/28 (60%)
Frame = +1 / +1
Query: 541 PFGGVKDSGFGRFAGVEGLRACCLVKSV 624
P+GG+K SGFGR G GL VK V
Sbjct: 625 PWGGIKRSGFGRELGEWGLDNYLSVKQV 708
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 1,092,550,725
Number of Sequences: 392609
Number of extensions: 14774785
Number of successful extensions: 50334
Number of sequences better than 1.0e-020: 6
length of database: 147,244,331
effective HSP length: 55
effective length of database: 125,650,836
effective search space used: 40082616684
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)