TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2622915.2.2
(562 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AW560211.1|AW560211 EST315251 DSIR Medicago truncatula c... 180 2e-044
gb|AJ497568.1|AJ497568 AJ497568 MTFLOW Medicago truncatula ... 180 2e-044
gb|CA920379.1|CA920379 EST638097 MTUS Medicago truncatula c... 180 2e-044
gb|BG449670.1|BG449670 NF055F06IN1F1057 Insect herbivory Me... 125 6e-037
gb|AC126785.11| Medicago truncatula clone mth2-9i24, comple... 106 2e-036
gb|AC146723.24| Medicago truncatula clone mth2-34n4, WORKIN... 106 5e-036
gb|CX541254.1|CX541254 s13dNF26D05GS046_465804 Germinating ... 115 5e-025
>gb|AW560211.1|AW560211 EST315251 DSIR Medicago truncatula cDNA clone pDSIR-26M4, mRNA
sequence
Length = 656
Score = 180 bits (387), Expect = 2e-044
Identities = 70/89 (78%), Positives = 73/89 (82%)
Frame = -3 / -2
Query: 548 LFLGFATXXXXAXTGPHLFTXWLXMVXRVXXTVEAHSGYHFPWSPSNFLPLYGGSDFHDY 369
LFLGFAT A TGPHL T WL MV RV TVEAH GYHFPWSPSNFLPLYGGSDFHDY
Sbjct: 535 LFLGFATIVGPAITGPHLITLWLWMVLRVLETVEAHCGYHFPWSPSNFLPLYGGSDFHDY 356
Query: 368 HHRVLYTKSGNYASTFVYMDWLFRTDNGY 282
HHR+LYTKSGNY+STF YMD +F TD GY
Sbjct: 355 HHRLLYTKSGNYSSTFTYMDRIFGTDIGY 269
>gb|AJ497568.1|AJ497568 AJ497568 MTFLOW Medicago truncatula cDNA clone mt--abc955111a03,
mRNA sequence
Length = 495
Score = 180 bits (387), Expect = 2e-044
Identities = 70/89 (78%), Positives = 73/89 (82%)
Frame = -3 / +1
Query: 548 LFLGFATXXXXAXTGPHLFTXWLXMVXRVXXTVEAHSGYHFPWSPSNFLPLYGGSDFHDY 369
LFLGFAT A TGPHL T WL MV RV TVEAH GYHFPWSPSNFLPLYGGSDFHDY
Sbjct: 115 LFLGFATIVGPAITGPHLITLWLWMVLRVLETVEAHCGYHFPWSPSNFLPLYGGSDFHDY 294
Query: 368 HHRVLYTKSGNYASTFVYMDWLFRTDNGY 282
HHR+LYTKSGNY+STF YMD +F TD GY
Sbjct: 295 HHRLLYTKSGNYSSTFTYMDRIFGTDIGY 381
>gb|CA920379.1|CA920379 EST638097 MTUS Medicago truncatula cDNA clone MTUS-27E3, mRNA
sequence
Length = 680
Score = 180 bits (387), Expect = 2e-044
Identities = 70/89 (78%), Positives = 73/89 (82%)
Frame = -3 / -2
Query: 548 LFLGFATXXXXAXTGPHLFTXWLXMVXRVXXTVEAHSGYHFPWSPSNFLPLYGGSDFHDY 369
LFLGFAT A TGPHL T WL MV RV TVEAH GYHFPWSPSNFLPLYGGSDFHDY
Sbjct: 454 LFLGFATIVGPAITGPHLITLWLWMVVRVLETVEAHCGYHFPWSPSNFLPLYGGSDFHDY 275
Query: 368 HHRVLYTKSGNYASTFVYMDWLFRTDNGY 282
HHR+LYTKSGNY+STF YMD +F TD GY
Sbjct: 274 HHRLLYTKSGNYSSTFTYMDRIFGTDVGY 188
Score = 112 bits (240), Expect = 4e-024
Identities = 53/84 (63%), Positives = 60/84 (71%)
Frame = +1 / +3
Query: 292 SVRNNQSM*TNVEA*FPDFVYSTR*W*SWKSEPPYSGRKFDGLHGK**PL*ASTVXXTLX 471
SV N +SM* NV+ *FPD VY+ R* *SWKS+PPY GRKF+GLHGK *P ASTV TL
Sbjct: 198 SVPNIRSM*VNVDE*FPDLVYNKR***SWKSDPPYKGRKFEGLHGKW*PQCASTVSKTLT 377
Query: 472 TXXSHXVNR*GPVXAXXXXVANPR 543
T +H V + GPV A VANPR
Sbjct: 378 TIHNHKVIKWGPVMAGPTMVANPR 449
>gb|BG449670.1|BG449670 NF055F06IN1F1057 Insect herbivory Medicago truncatula cDNA clone
NF055F06IN 5', mRNA sequence
Length = 664
Score = 125 bits (268), Expect(2) = 6e-037
Identities = 45/53 (84%), Positives = 49/53 (92%)
Frame = -3 / -1
Query: 440 SGYHFPWSPSNFLPLYGGSDFHDYHHRVLYTKSGNYASTFVYMDWLFRTDNGY 282
+GYHFPWSPSNFLPLYGGSDFHDYHHR+LYTKSGNY+STF YMD +F TD GY
Sbjct: 526 AGYHFPWSPSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFTYMDRIFGTDVGY 368
Score = 49.6 bits (102), Expect(2) = 6e-037
Identities = 24/40 (60%), Positives = 24/40 (60%)
Frame = -3 / -3
Query: 545 FLGFATXXXXAXTGPHLFTXWLXMVXRVXXTVEAHSGYHF 426
F AT A TGPHL T WL MV RV TVEAH G F
Sbjct: 632 FSWVATIVGPAITGPHLKTLWLXMVVRVLETVEAHCGLPF 513
>gb|AC126785.11| Medicago truncatula clone mth2-9i24, complete sequence
Length = 132702
Score = 106 bits (226), Expect(2) = 2e-036
Identities = 42/54 (77%), Positives = 42/54 (77%)
Frame = -3 / +2
Query: 548 LFLGFATXXXXAXTGPHLFTXWLXMVXRVXXTVEAHSGYHFPWSPSNFLPLYGG 387
LFLGFAT A TGPHL T WL MV RV TVEAH GYHFPWSPSNFLPLYGG
Sbjct: 103970 LFLGFATIVGPAITGPHLITLWLWMVVRVLETVEAHCGYHFPWSPSNFLPLYGG 104131
Score = 67.0 bits (140), Expect(2) = 2e-036
Identities = 24/31 (77%), Positives = 27/31 (87%)
Frame = -3 / +2
Query: 401 PLYGGSDFHDYHHRVLYTKSGNYASTFVYMD 309
P + SDFHDYHHR+LYTKSGNY+STF YMD
Sbjct: 104216 PQFFRSDFHDYHHRLLYTKSGNYSSTFTYMD 104308
>gb|AC146723.24| Medicago truncatula clone mth2-34n4, WORKING DRAFT SEQUENCE, 3 ordered
pieces
Length = 121085
Score = 106 bits (226), Expect(2) = 5e-036
Identities = 42/54 (77%), Positives = 42/54 (77%)
Frame = -3 / +2
Query: 548 LFLGFATXXXXAXTGPHLFTXWLXMVXRVXXTVEAHSGYHFPWSPSNFLPLYGG 387
LFLGFAT A TGPHL T WL MV RV TVEAH GYHFPWSPSNFLPLYGG
Sbjct: 85715 LFLGFATIVGPAITGPHLITLWLWMVLRVLETVEAHCGYHFPWSPSNFLPLYGG 85876
Score = 65.7 bits (137), Expect(2) = 5e-036
Identities = 23/26 (88%), Positives = 25/26 (96%)
Frame = -3 / +2
Query: 386 SDFHDYHHRVLYTKSGNYASTFVYMD 309
SDFHDYHHR+LYTKSGNY+STF YMD
Sbjct: 85976 SDFHDYHHRLLYTKSGNYSSTFTYMD 86053
>gb|CX541254.1|CX541254 s13dNF26D05GS046_465804 Germinating Seed Medicago truncatula cDNA,
mRNA sequence
Length = 479
Score = 115 bits (246), Expect = 5e-025
Identities = 42/49 (85%), Positives = 45/49 (91%)
Frame = -3 / +1
Query: 428 FPWSPSNFLPLYGGSDFHDYHHRVLYTKSGNYASTFVYMDWLFRTDNGY 282
FPWSPSNFLPLYGGSDFHDYHHR+LYTKSGNY+STF YMD +F TD GY
Sbjct: 4 FPWSPSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFTYMDRIFGTDVGY 150
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 429,477,948
Number of Sequences: 392609
Number of extensions: 5150094
Number of successful extensions: 42175
Number of sequences better than 1.0e-020: 9
length of database: 147,244,331
effective HSP length: 53
effective length of database: 126,436,054
effective search space used: 16815995182
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)