TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2619146.2.1
(636 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AL382396.1|AL382396 MtBC06E08R1 MtBC Medicago truncatula... 139 2e-033
gb|BG448529.1|BG448529 NF036G02RT1F1017 Developing root Med... 129 3e-030
gb|AL375055.1|AL375055 MtBB11A08R1 MtBB Medicago truncatula... 132 7e-030
gb|BG450908.1|BG450908 NF095E08DT1F1065 Drought Medicago tr... 127 3e-029
gb|AC138171.17| Medicago truncatula clone mth2-7f10, comple... 77 2e-026
gb|AC147428.6| Medicago truncatula clone mth2-34i10, comple... 71 6e-023
gb|AC166046.6| Medicago truncatula clone mth2-6p7, WORKING ... 73 1e-021
gb|AC170856.1| Medicago truncatula chromosome 2 clone mth2-... 71 3e-021
>gb|AL382396.1|AL382396 MtBC06E08R1 MtBC Medicago truncatula cDNA clone MtBC06E08 T7, mRNA
sequence
Length = 526
Score = 139 bits (299), Expect(2) = 2e-033
Identities = 56/97 (57%), Positives = 70/97 (72%)
Frame = -3 / +1
Query: 586 FANSAGFDILETTMVNIQDLPLEAVLDEEGQKALSAQLPAIMQQGLAYLPGGVCRSSMGR 407
F+N AG D+LETT+V +QD+ L+ VLDE G+K L ++ IMQQG A LPGG+C SSM R
Sbjct: 58 FSNQAGLDMLETTLVALQDIMLDKVLDEAGRKILCSEFSKIMQQGYASLPGGICVSSMNR 237
Query: 406 QASYEQAVAWKVVGDDGAPQCLALMLANWTFI*GSQH 296
SYEQA+AWKV+ DD A CLA M NW+F+* H
Sbjct: 238 PVSYEQAIAWKVLNDDDANHCLAFMFINWSFV*WRNH 348
Score = 23.5 bits (45), Expect(2) = 2e-033
Identities = 8/12 (66%), Positives = 10/12 (83%)
Frame = -3 / +1
Query: 634 DAILCCSLKPPA 599
DAI+CCS+K A
Sbjct: 7 DAIMCCSVKTNA 42
>gb|BG448529.1|BG448529 NF036G02RT1F1017 Developing root Medicago truncatula cDNA clone
NF036G02RT 5', mRNA sequence
Length = 468
Score = 129 bits (276), Expect(2) = 3e-030
Identities = 49/93 (52%), Positives = 66/93 (70%)
Frame = -3 / +1
Query: 586 FANSAGFDILETTMVNIQDLPLEAVLDEEGQKALSAQLPAIMQQGLAYLPGGVCRSSMGR 407
F+N AG D+LETT+V +QD+ LE + D+ G+K L ++ P I+QQG A L GG+C SSMGR
Sbjct: 169 FSNQAGLDMLETTLVALQDISLEKIFDDNGRKTLFSEFPQIIQQGFACLQGGICLSSMGR 348
Query: 406 QASYEQAVAWKVVGDDGAPQCLALMLANWTFI* 308
SYE+AVAWKV+ D+ C+ M NW F+*
Sbjct: 349 PVSYERAVAWKVLNDEQNAHCICFMFVNWXFV* 447
Score = 23.5 bits (45), Expect(2) = 3e-030
Identities = 7/9 (77%), Positives = 9/9 (100%)
Frame = -3 / +1
Query: 634 DAILCCSLK 608
DA+LCC+LK
Sbjct: 124 DAVLCCTLK 150
>gb|AL375055.1|AL375055 MtBB11A08R1 MtBB Medicago truncatula cDNA clone MtBB11A08 T7, mRNA
sequence
Length = 535
Score = 132 bits (282), Expect = 7e-030
Identities = 53/92 (57%), Positives = 66/92 (71%)
Frame = -3 / +1
Query: 571 GFDILETTMVNIQDLPLEAVLDEEGQKALSAQLPAIMQQGLAYLPGGVCRSSMGRQASYE 392
G D+LETT+V +QD+ L+ VLDE G+K L ++ IMQQG A LPGG+C SSM R SYE
Sbjct: 1 GLDMLETTLVALQDIMLDKVLDEAGRKILCSEFSKIMQQGYASLPGGICVSSMNRPVSYE 180
Query: 391 QAVAWKVVGDDGAPQCLALMLANWTFI*GSQH 296
QA+AWKV+ DD A CLA M NW+F+* H
Sbjct: 181 QAIAWKVLNDDDANHCLAFMFINWSFV*WRNH 276
>gb|BG450908.1|BG450908 NF095E08DT1F1065 Drought Medicago truncatula cDNA clone NF095E08DT
5', mRNA sequence
Length = 694
Score = 127 bits (271), Expect(2) = 3e-029
Identities = 47/93 (50%), Positives = 66/93 (70%)
Frame = -3 / -1
Query: 586 FANSAGFDILETTMVNIQDLPLEAVLDEEGQKALSAQLPAIMQQGLAYLPGGVCRSSMGR 407
FAN AG D+LETT+V +QD+ LE + D+ G+K L + P IMQQG + GG+C SSMGR
Sbjct: 493 FANQAGLDMLETTLVALQDITLEKIFDDNGKKTLCXEFPQIMQQGFMCIQGGICLSSMGR 314
Query: 406 QASYEQAVAWKVVGDDGAPQCLALMLANWTFI* 308
+YE+AVAWKV+ ++ + C+ M NW+F+*
Sbjct: 313 PITYERAVAWKVLNEEESAHCICFMFINWSFV* 215
Score = 22.6 bits (43), Expect(2) = 3e-029
Identities = 7/9 (77%), Positives = 8/9 (88%)
Frame = -3 / -1
Query: 634 DAILCCSLK 608
DA+ CCSLK
Sbjct: 538 DAVXCCSLK 512
>gb|AC138171.17| Medicago truncatula clone mth2-7f10, complete sequence
Length = 104336
Score = 77.1 bits (162), Expect(3) = 2e-026
Identities = 29/50 (58%), Positives = 36/50 (72%)
Frame = -3 / +2
Query: 457 QGLAYLPGGVCRSSMGRQASYEQAVAWKVVGDDGAPQCLALMLANWTFI* 308
QG A +P G+C S+MGR ASY+QAVAWKV +D + CLA NW+FI*
Sbjct: 82934 QGFACMPAGICMSTMGRHASYDQAVAWKVHAEDNSVHCLAFSFINWSFI* 83083
Score = 55.6 bits (115), Expect(3) = 2e-026
Identities = 22/48 (45%), Positives = 33/48 (68%)
Frame = -3 / +2
Query: 586 FANSAGFDILETTMVNIQDLPLEAVLDEEGQKALSAQLPAIMQQGLAY 443
FAN AG D+LETT+V +QD+ L+ + DE +K L A +MQQ +++
Sbjct: 82676 FANQAGLDMLETTLVALQDITLDKIFDESARKNLIAYFAKLMQQVISW 82819
Score = 25.4 bits (49), Expect(3) = 2e-026
Identities = 9/9 (100%), Positives = 9/9 (100%)
Frame = -3 / +2
Query: 634 DAILCCSLK 608
DAILCCSLK
Sbjct: 82523 DAILCCSLK 82549
>gb|AC147428.6| Medicago truncatula clone mth2-34i10, complete sequence
Length = 112873
Score = 71.2 bits (149), Expect(3) = 6e-023
Identities = 25/53 (47%), Positives = 37/53 (69%)
Frame = -3 / +2
Query: 466 IMQQGLAYLPGGVCRSSMGRQASYEQAVAWKVVGDDGAPQCLALMLANWTFI* 308
I+ QG + GG+C SSMGR +YE+AVAWKV+ ++ + C+ M NW+F+*
Sbjct: 45218 ILVQGFMCIQGGICLSSMGRPITYERAVAWKVLNEEESAHCICFMFINWSFV* 45376
Score = 63.4 bits (132), Expect(3) = 6e-023
Identities = 24/44 (54%), Positives = 33/44 (75%)
Frame = -3 / +2
Query: 586 FANSAGFDILETTMVNIQDLPLEAVLDEEGQKALSAQLPAIMQQ 455
FAN AG D+LETT+V +QD+ LE + D+ G+K L ++ P IMQQ
Sbjct: 44918 FANQAGLDMLETTLVALQDITLEKIFDDNGKKTLCSEFPQIMQQ 45049
Score = 28.6 bits (56), Expect(3) = 6e-023
Identities = 10/17 (58%), Positives = 14/17 (82%)
Frame = -3 / +1
Query: 634 DAILCCSLKPPAFTLKF 584
DA+LCCSLK +F++ F
Sbjct: 44719 DAVLCCSLKVISFSIFF 44769
>gb|AC166046.6| Medicago truncatula clone mth2-6p7, WORKING DRAFT SEQUENCE, 8
unordered pieces
Length = 118925
Score = 73.0 bits (153), Expect(2) = 1e-021
Identities = 27/49 (55%), Positives = 34/49 (69%)
Frame = -3 / +2
Query: 454 GLAYLPGGVCRSSMGRQASYEQAVAWKVVGDDGAPQCLALMLANWTFI* 308
G A L GG+C SSMGR SYE+AVAWKV+ D+ C+ M NW+F+*
Sbjct: 9425 GFACLQGGICLSSMGRPVSYERAVAWKVLNDEQNAHCICFMFVNWSFV* 9571
Score = 60.2 bits (125), Expect(2) = 1e-021
Identities = 23/49 (46%), Positives = 35/49 (71%)
Frame = -3 / +2
Query: 586 FANSAGFDILETTMVNIQDLPLEAVLDEEGQKALSAQLPAIMQQGLAYL 440
F+N AG D+LETT+V +QD+ LE + D+ G+K L ++ P I+QQ +L
Sbjct: 9137 FSNQAGLDMLETTLVALQDISLEKIFDDNGRKTLFSEFPQIIQQVYNFL 9283
>gb|AC170856.1| Medicago truncatula chromosome 2 clone mth2-13d14, *** SEQUENCING IN
PROGRESS ***, 2 ordered pieces
Length = 101063
Score = 70.7 bits (148), Expect(2) = 3e-021
Identities = 26/53 (49%), Positives = 36/53 (67%)
Frame = -3 / -3
Query: 466 IMQQGLAYLPGGVCRSSMGRQASYEQAVAWKVVGDDGAPQCLALMLANWTFI* 308
++ QG A L GG+C SSMGR SYE+ VAWKV+ ++ C+ M NW+F+*
Sbjct: 70578 LIWQGFACLQGGLCLSSMGRPISYERVVAWKVLNEEENAHCICFMFVNWSFV* 70420
Score = 61.1 bits (127), Expect(2) = 3e-021
Identities = 23/44 (52%), Positives = 33/44 (75%)
Frame = -3 / -1
Query: 586 FANSAGFDILETTMVNIQDLPLEAVLDEEGQKALSAQLPAIMQQ 455
FAN AG D+LETT+V +QD+ LE + D+ G+K L ++ P I+QQ
Sbjct: 70871 FANQAGLDMLETTLVALQDIALEKIFDDHGRKILCSEFPLIIQQ 70740
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 479,192,064
Number of Sequences: 392609
Number of extensions: 6139790
Number of successful extensions: 44710
Number of sequences better than 1.0e-020: 12
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 19788820865
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)