TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2594405.2.1
(637 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CB065510.1|CB065510 EST645191 HOGA Medicago truncatula c... 270 2e-071
emb|CR321775.1| mte1-48F20FM1 BAC end, cultivar Jemalong A1... 253 1e-066
gb|AL382658.1|AL382658 MtBC09C03R1 MtBC Medicago truncatula... 163 2e-039
gb|AW171645.2|AW171645 N100539e rootphos(-) Medicago trunca... 152 4e-036
>gb|CB065510.1|CB065510 EST645191 HOGA Medicago truncatula cDNA clone HOGA-19K13, mRNA
sequence
Length = 650
Score = 270 bits (583), Expect = 2e-071
Identities = 102/164 (62%), Positives = 136/164 (82%)
Frame = -2 / -1
Query: 636 DRGAPVGGIGVQGHISHPVGEIICDSLDKLAILGLPIWITELDVTAENEHIRADDLEVFL 457
++GAPVGGIG+QGHI PVG ++C SLDKL ILGLPIW TELDV++ NE++R DDLEV L
Sbjct: 641 EQGAPVGGIGIQGHIDSPVGPVVCSSLDKLGILGLPIWFTELDVSSMNEYVRGDDLEVML 462
Query: 456 REAFAHPAVGGIILWGFWEMFMFREHAHLVDADGTINEAGRRYLALKQEWLTRMNGSVDH 277
REA AHPAV GI+LWGFWE+FM R++AHLV+A+G INEAG+R+LALKQEWL+ +G V+
Sbjct: 461 REAMAHPAVEGIMLWGFWELFMSRDNAHLVNAEGDINEAGKRFLALKQEWLSHSHGHVNE 282
Query: 276 QGEFKFRGYHGSYTVEVNTPSGKVARSFVVDKDSPVQVIALNV* 145
QG+F FRG++G+Y VE+ TPS K+ ++FV+DK +++++*
Sbjct: 281 QGQFNFRGFYGTYNVEIVTPSKKIFKTFVLDKGDSPMEVSIDL* 150
Score = 133 bits (286), Expect = 2e-030
Identities = 72/152 (47%), Positives = 84/152 (55%)
Frame = +2 / +1
Query: 182 LSTTNDLATLPEGVFTSTVYEPWYPLNLNSPW*STLPFIRVSHSCLRARYLLPASLIVPS 361
LS+TN L +GV ST+Y P PL LN P T P + SHSC +AR L PASLI PS
Sbjct: 187 LSSTNVLKIFFDGVTISTLYVP*NPLKLN*PCSLT*P*L*DSHSCFKARNLFPASLISPS 366
Query: 362 ASTRWACSRNINISQNPQRMIPPTAGCXXXXXXXXXXXXXXMCSFSAVTSNSVIQIGRPS 541
A T+ A S IN SQNP +IP TAG SF TS+SV IG+P
Sbjct: 367 AFTKCALSLLINNSQNPHNIIPSTAG*AMASLSITSRSSPLTYSFIEDTSSSVNHIGKPR 546
Query: 542 MASLSRESHMISPTGWLI*PCTPIPPTGAPLS 637
+ +LS E PTG + P PIPPTGAP S
Sbjct: 547 IPNLSSEEQTTGPTGLSMCP*IPIPPTGAPCS 642
>emb|CR321775.1| mte1-48F20FM1 BAC end, cultivar Jemalong A17 of Medicago
truncatula, genomic survey sequence
Length = 732
Score = 253 bits (548), Expect = 1e-066
Identities = 99/164 (60%), Positives = 130/164 (79%)
Frame = -2 / +3
Query: 636 DRGAPVGGIGVQGHISHPVGEIICDSLDKLAILGLPIWITELDVTAENEHIRADDLEVFL 457
D+GA VGGIG+QGHI PVG I+C +LDKLA LG PIW TELDV++ NE++R DDLEV L
Sbjct: 48 DQGASVGGIGIQGHIDSPVGPIVCAALDKLATLGQPIWFTELDVSSTNEYVRGDDLEVML 227
Query: 456 REAFAHPAVGGIILWGFWEMFMFREHAHLVDADGTINEAGRRYLALKQEWLTRMNGSVDH 277
REA AHPAV G++LWGFWE+FM R++AHLV+A+G INEAGRR++ LK+EWL+ +G +D
Sbjct: 228 REAMAHPAVDGVMLWGFWELFMSRDNAHLVNAEGEINEAGRRFIDLKEEWLSHTHGHLDE 407
Query: 276 QGEFKFRGYHGSYTVEVNTPSGKVARSFVVDKDSPVQVIALNV* 145
QGEF FRG+ G Y VE+ T + ++FVVDK + VI++++*
Sbjct: 408 QGEFNFRGFSGLYNVEIVTFEKVICQTFVVDKGNSSLVISIDL* 539
Score = 127 bits (272), Expect = 2e-028
Identities = 67/137 (48%), Positives = 79/137 (57%)
Frame = +2 / -3
Query: 221 VFTSTVYEPWYPLNLNSPW*STLPFIRVSHSCLRARYLLPASLIVPSASTRWACSRNINI 400
V ST+Y+P PL LNSP S P + +HS LR+ LLPASLI PSA T+ A SR IN
Sbjct: 463 VTISTLYKPLNPLKLNSPCSSR*P*V*DNHSSLRSMNLLPASLISPSAFTKCALSRLINN 284
Query: 401 SQNPQRMIPPTAGCXXXXXXXXXXXXXXMCSFSAVTSNSVIQIGRPSMASLSRESHMISP 580
SQNP + P TAGC S TS+SV QIG P +A+LS + I P
Sbjct: 283 SQNPHSITPSTAGCAIASRNITSKSSPRTYSLVEDTSSSVNQIG*PRVANLSSAAQTIGP 104
Query: 581 TGWLI*PCTPIPPTGAP 631
TG +*P PIPPT AP
Sbjct: 103 TGLSM*P*IPIPPTDAP 53
>gb|AL382658.1|AL382658 MtBC09C03R1 MtBC Medicago truncatula cDNA clone MtBC09C03 T7, mRNA
sequence
Length = 491
Score = 163 bits (351), Expect = 2e-039
Identities = 61/105 (58%), Positives = 87/105 (82%)
Frame = -2 / +1
Query: 459 LREAFAHPAVGGIILWGFWEMFMFREHAHLVDADGTINEAGRRYLALKQEWLTRMNGSVD 280
LREA AHPAV GI+LWGFWE+FM R++AHLV+A+G INEAG+R+LALKQEWL+ +G V+
Sbjct: 4 LREAMAHPAVEGIMLWGFWELFMSRDNAHLVNAEGDINEAGKRFLALKQEWLSHSHGHVN 183
Query: 279 HQGEFKFRGYHGSYTVEVNTPSGKVARSFVVDKDSPVQVIALNV* 145
QG+F FRG++G+Y VE+ TPS K++++FV+DK +++++*
Sbjct: 184 EQGQFNFRGFYGTYNVEIVTPSKKISKTFVLDKGDSPMEVSIDL* 318
>gb|AW171645.2|AW171645 N100539e rootphos(-) Medicago truncatula cDNA clone MHRP-8B8, mRNA
sequence
Length = 547
Score = 152 bits (327), Expect = 4e-036
Identities = 57/78 (73%), Positives = 67/78 (85%)
Frame = -2 / +1
Query: 636 DRGAPVGGIGVQGHISHPVGEIICDSLDKLAILGLPIWITELDVTAENEHIRADDLEVFL 457
++GAPVGGIG+QGHI PVG ++C SLDKL ILGLPIW TELDV++ NE++R DDLEV L
Sbjct: 313 EQGAPVGGIGIQGHIDSPVGPVVCSSLDKLGILGLPIWFTELDVSSMNEYVRGDDLEVML 492
Query: 456 REAFAHPAVGGIILWGFW 403
REA AHPAV GI+LWGFW
Sbjct: 493 REAMAHPAVEGIMLWGFW 546
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 689,346,451
Number of Sequences: 392609
Number of extensions: 10008509
Number of successful extensions: 107605
Number of sequences better than 1.0e-020: 4
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 19788820865
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)