TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2594147.2.1
(744 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG453428.1|BG453428 NF091G11LF1F1085 Developing leaf Med... 98 8e-036
gb|BG455193.1|BG455193 NF100G04PL1F1034 Phosphate starved l... 94 4e-028
gb|AL379968.1|AL379968 MtBB48E05F1 MtBB Medicago truncatula... 94 8e-022
gb|CA917715.1|CA917715 EST641862 GPOD Medicago truncatula c... 94 1e-021
>gb|BG453428.1|BG453428 NF091G11LF1F1085 Developing leaf Medicago truncatula cDNA clone
NF091G11LF 5', mRNA sequence
Length = 638
Score = 98.2 bits (208), Expect(3) = 8e-036
Identities = 37/80 (46%), Positives = 55/80 (68%)
Frame = -1 / +3
Query: 723 DEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNYL 544
D + HFD+SG+AFG++A G++ +LR AG I I++ RVQC + G + F V+ GSN Y
Sbjct: 15 DAEYHFDLSGSAFGSMAVSGEDEELRNAGKIVIEHRRVQCNYGGSTIAFHVDSGSNQEYF 194
Query: 543 AVLIEYEDSDSALDSVDMMQ 484
A LIEYE+ D L+ V++ +
Sbjct: 195 AALIEYEEGDGDLNKVELKE 254
Score = 51.0 bits (105), Expect(3) = 8e-036
Identities = 18/32 (56%), Positives = 23/32 (71%)
Frame = -1 / +3
Query: 468 TGQWVPMQQSWGAVWKINSGSVLQGPFNIRLT 373
+ W MQQSWGAVWK + G+ L+ P +IRLT
Sbjct: 264 SSSWDTMQQSWGAVWKFDKGAPLKAPISIRLT 359
Score = 40.9 bits (83), Expect(3) = 8e-036
Identities = 15/26 (57%), Positives = 17/26 (65%)
Frame = -1 / +3
Query: 375 TFSSGRALVASNAIPAGWNAGVAYRS 298
T SG+ +VA N IPAGW G YRS
Sbjct: 360 TLKSGKTIVAHNVIPAGWKPGQTYRS 437
>gb|BG455193.1|BG455193 NF100G04PL1F1034 Phosphate starved leaf Medicago truncatula cDNA
clone NF100G04PL 5', mRNA sequence
Length = 654
Score = 94.1 bits (199), Expect(3) = 4e-028
Identities = 34/82 (41%), Positives = 52/82 (63%)
Frame = -1 / +2
Query: 729 CPDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPN 550
CP ++ HFD+SG AFG +A G+ QLR G IQ+ Y R C + G ++ F V GS P
Sbjct: 188 CPSDRTHFDLSGAAFGHMAVHGENGQLRNRGEIQVLYRRTTCVYAGKNIAFQVNEGSTPF 367
Query: 549 YLAVLIEYEDSDSALDSVDMMQ 484
+L++L+E+ED D + S+ + +
Sbjct: 368 WLSLLVEFEDGDGDISSMHIQE 433
Score = 39.1 bits (79), Expect(3) = 4e-028
Identities = 12/29 (41%), Positives = 24/29 (82%)
Frame = -1 / +2
Query: 402 LQGPFNIRLTFSSGRALVASNAIPAGWNA 316
L+GPF+++L+ S+G++L A + IP+ W++
Sbjct: 503 LRGPFSVKLSTSTGKSLTAKDVIPSHWSS 589
Score = 30.9 bits (61), Expect(3) = 4e-028
Identities = 10/30 (33%), Positives = 15/30 (50%)
Frame = -1 / +2
Query: 498 VDMMQTQGGTTGQWVPMQQSWGAVWKINSG 409
+ M Q + +W+ M WGA W I +G
Sbjct: 410 ISSMHIQEAGSSEWLQMNHLWGANWNIVTG 499
>gb|AL379968.1|AL379968 MtBB48E05F1 MtBB Medicago truncatula cDNA clone MtBB48E05 T3, mRNA
sequence
Length = 476
Score = 94.1 bits (199), Expect(2) = 8e-022
Identities = 34/82 (41%), Positives = 52/82 (63%)
Frame = -1 / +2
Query: 729 CPDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPN 550
CP ++ HFD+SG AFG +A G+ QLR G IQ+ Y R C + G ++ F V GS P
Sbjct: 116 CPSDRTHFDLSGAAFGHMAVHGENGQLRNRGEIQVLYRRTTCVYAGKNIAFQVNEGSTPF 295
Query: 549 YLAVLIEYEDSDSALDSVDMMQ 484
+L++L+E+ED D + S+ + +
Sbjct: 296 WLSLLVEFEDGDGDISSMHIQE 361
Score = 30.9 bits (61), Expect(2) = 8e-022
Identities = 10/30 (33%), Positives = 15/30 (50%)
Frame = -1 / +2
Query: 498 VDMMQTQGGTTGQWVPMQQSWGAVWKINSG 409
+ M Q + +W+ M WGA W I +G
Sbjct: 338 ISSMHIQEAGSSEWLQMNHLWGANWNIVTG 427
>gb|CA917715.1|CA917715 EST641862 GPOD Medicago truncatula cDNA clone GPOD-37E9, mRNA
sequence
Length = 769
Score = 94.1 bits (199), Expect(2) = 1e-021
Identities = 34/82 (41%), Positives = 52/82 (63%)
Frame = -1 / +2
Query: 729 CPDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPN 550
CP ++ HFD+SG AFG +A G+ QLR G IQ+ Y R C + G ++ F V GS P
Sbjct: 440 CPSDRTHFDLSGAAFGHMAVHGENGQLRNRGEIQVLYRRTTCVYAGKNIAFQVNEGSTPF 619
Query: 549 YLAVLIEYEDSDSALDSVDMMQ 484
+L++L+E+ED D + S+ + +
Sbjct: 620 WLSLLVEFEDGDGDISSMHIQE 685
Score = 30.4 bits (60), Expect(2) = 1e-021
Identities = 8/27 (29%), Positives = 14/27 (51%)
Frame = -1 / +2
Query: 498 VDMMQTQGGTTGQWVPMQQSWGAVWKI 418
+ M Q + +W+ M WGA+W +
Sbjct: 662 ISSMHIQEAGSSEWLQMNHLWGAIWNM 742
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 604,905,589
Number of Sequences: 392609
Number of extensions: 7051000
Number of successful extensions: 39845
Number of sequences better than 1.0e-020: 6
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 24326384885
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)