TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2550809.2.1
(876 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DW018148.1|DW018148 EST1227109 MTY Medicago truncatula c... 162 1e-049
gb|CA858106.1|CA858106 EST635361 GLSD Medicago truncatula c... 162 1e-049
gb|BF645058.1|BF645058 NF019G03EC1F1023 Elicited cell cultu... 162 1e-048
gb|AC168147.11| Medicago truncatula clone mth2-69l5, WORKIN... 123 6e-038
>gb|DW018148.1|DW018148 EST1227109 MTY Medicago truncatula cDNA clone MTYB288, mRNA
sequence
Length = 837
Score = 162 bits (349), Expect(2) = 1e-049
Identities = 64/137 (46%), Positives = 95/137 (69%)
Frame = -1 / +2
Query: 789 YSAMVFLEAEMSWNVLISPSQLDRKGLLLRKAIIVRLLEDVTHRRASKEHGYYIAVNQLK 610
++ +FL+ ++ WNV+I+ L L+L++AI++RLL D ++A+K+ GY++AV L
Sbjct: 167 FTRTMFLKVQLPWNVIIAAENLKPGSLMLQRAILIRLLSDFAAKKATKDMGYFLAVTTLD 346
Query: 609 AISEGKVRELTGDVLFPVSFTCITQKPMKGEVMVGHVDRILKHGVFLKSGPVESIFLAEK 430
I EGKVR+ TGDVLFPV F +T K KGEV+ G V ++LKHGVF++ GP+E+ +L+
Sbjct: 347 KIGEGKVRQHTGDVLFPVVFNAVTFKIFKGEVLEGVVHKVLKHGVFMRIGPIENAYLSSS 526
Query: 429 SMSNYKYIGGENAMFMN 379
M Y Y+ GEN FMN
Sbjct: 527 KMPGYVYVLGENPYFMN 577
Score = 55.6 bits (115), Expect(2) = 1e-049
Identities = 20/38 (52%), Positives = 28/38 (73%)
Frame = -1 / +2
Query: 369 KLEKDTAVRFKVLGFRWMEADRQFQLLATIAGDFLGPL 256
K+ KD VR V+G +WMEA+R+FQ L + GD+LGP+
Sbjct: 590 KIAKDVKVRVVVIGTKWMEAEREFQALVGLEGDYLGPI 703
>gb|CA858106.1|CA858106 EST635361 GLSD Medicago truncatula cDNA clone pGLSD-27C7, mRNA
sequence
Length = 724
Score = 162 bits (349), Expect(2) = 1e-049
Identities = 64/137 (46%), Positives = 95/137 (69%)
Frame = -1 / +2
Query: 789 YSAMVFLEAEMSWNVLISPSQLDRKGLLLRKAIIVRLLEDVTHRRASKEHGYYIAVNQLK 610
++ +FL+ ++ WNV+I+ L L+L++AI++RLL D ++A+K+ GY++AV L
Sbjct: 44 FTRTMFLKVQLPWNVIIAAENLKPGSLMLQRAILIRLLSDFAAKKATKDMGYFLAVTTLD 223
Query: 609 AISEGKVRELTGDVLFPVSFTCITQKPMKGEVMVGHVDRILKHGVFLKSGPVESIFLAEK 430
I EGKVR+ TGDVLFPV F +T K KGEV+ G V ++LKHGVF++ GP+E+ +L+
Sbjct: 224 KIGEGKVRQHTGDVLFPVVFNAVTFKIFKGEVLEGVVHKVLKHGVFMRIGPIENAYLSSS 403
Query: 429 SMSNYKYIGGENAMFMN 379
M Y Y+ GEN FMN
Sbjct: 404 KMPGYVYVLGENPYFMN 454
Score = 55.6 bits (115), Expect(2) = 1e-049
Identities = 20/38 (52%), Positives = 28/38 (73%)
Frame = -1 / +2
Query: 369 KLEKDTAVRFKVLGFRWMEADRQFQLLATIAGDFLGPL 256
K+ KD VR V+G +WMEA+R+FQ L + GD+LGP+
Sbjct: 467 KIAKDVKVRVVVIGTKWMEAEREFQALVGLEGDYLGPI 580
>gb|BF645058.1|BF645058 NF019G03EC1F1023 Elicited cell culture Medicago truncatula cDNA
clone NF019G03EC 5', mRNA sequence
Length = 686
Score = 162 bits (349), Expect(2) = 1e-048
Identities = 64/137 (46%), Positives = 95/137 (69%)
Frame = -1 / +3
Query: 789 YSAMVFLEAEMSWNVLISPSQLDRKGLLLRKAIIVRLLEDVTHRRASKEHGYYIAVNQLK 610
++ +FL+ ++ WNV+I+ L L+L++AI++RLL D ++A+K+ GY++AV L
Sbjct: 99 FTRTMFLKVQLPWNVIIAAENLKPGSLMLQRAILIRLLSDFAAKKATKDMGYFLAVTTLD 278
Query: 609 AISEGKVRELTGDVLFPVSFTCITQKPMKGEVMVGHVDRILKHGVFLKSGPVESIFLAEK 430
I EGKVR+ TGDVLFPV F +T K KGEV+ G V ++LKHGVF++ GP+E+ +L+
Sbjct: 279 KIGEGKVRQHTGDVLFPVVFNAVTFKIFKGEVLEGVVHKVLKHGVFMRIGPIENAYLSSS 458
Query: 429 SMSNYKYIGGENAMFMN 379
M Y Y+ GEN FMN
Sbjct: 459 KMPGYVYVLGENPYFMN 509
Score = 52.4 bits (108), Expect(2) = 1e-048
Identities = 19/38 (50%), Positives = 27/38 (71%)
Frame = -1 / +3
Query: 369 KLEKDTAVRFKVLGFRWMEADRQFQLLATIAGDFLGPL 256
K+ KD VR V+G +WMEA+R+FQ L + GD+L P+
Sbjct: 522 KIAKDVKVRVVVIGTKWMEAEREFQALVGLEGDYLXPI 635
>gb|AC168147.11| Medicago truncatula clone mth2-69l5, WORKING DRAFT SEQUENCE, 4
unordered pieces
Length = 117771
Score = 123 bits (264), Expect(2) = 6e-038
Identities = 49/94 (52%), Positives = 67/94 (71%)
Frame = -1 / -2
Query: 660 RRASKEHGYYIAVNQLKAISEGKVRELTGDVLFPVSFTCITQKPMKGEVMVGHVDRILKH 481
++A+K+ GY++AV L I EGKVR+ TGDVLFPV F +T K KGEV+ G V ++LKH
Sbjct: 23282 KKATKDMGYFLAVTTLDKIGEGKVRQHTGDVLFPVVFNAVTFKIFKGEVLEGVVHKVLKH 23103
Query: 480 GVFLKSGPVESIFLAEKSMSNYKYIGGENAMFMN 379
GVF++ GP+E+ +L+ M Y Y+ GEN FMN
Sbjct: 23102 GVFMRIGPIENAYLSSSKMPGYVYVLGENPYFMN 23001
Score = 55.6 bits (115), Expect(2) = 6e-038
Identities = 20/38 (52%), Positives = 28/38 (73%)
Frame = -1 / -2
Query: 369 KLEKDTAVRFKVLGFRWMEADRQFQLLATIAGDFLGPL 256
K+ KD VR V+G +WMEA+R+FQ L + GD+LGP+
Sbjct: 22988 KIAKDVKVRVVVIGTKWMEAEREFQALVGLEGDYLGPI 22875
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 842,383,793
Number of Sequences: 392609
Number of extensions: 11849989
Number of successful extensions: 48475
Number of sequences better than 1.0e-020: 6
length of database: 147,244,331
effective HSP length: 55
effective length of database: 125,650,836
effective search space used: 29653597296
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)