TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2521459.2.2
(850 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA919619.1|CA919619 EST637337 MTUS Medicago truncatula c... 184 2e-045
gb|BM780235.1|BM780235 EST590811 KV2 Medicago truncatula cD... 100 3e-043
gb|CA923174.1|CA923174 EST640892 MTUS Medicago truncatula c... 136 4e-031
gb|CA923195.1|CA923195 EST640913 MTUS Medicago truncatula c... 136 4e-031
gb|AW774625.1|AW774625 EST333776 KV3 Medicago truncatula cD... 127 3e-028
gb|BI273174.1|BI273174 NF091B12FL1F1096 Developing flower M... 127 3e-028
gb|CX532800.1|CX532800 s13dNF71G05MJ036_271883 Methyl Jasmo... 127 3e-028
gb|AC147482.2| Medicago truncatula chromosome 7 BAC clone m... 122 6e-027
gb|AW775842.1|AW775842 EST334907 DSIL Medicago truncatula c... 110 4e-023
gb|BI309356.1|BI309356 EST530766 GPOD Medicago truncatula c... 109 6e-023
gb|AC128660.31| Medicago truncatula clone mth2-14p23, compl... 48 6e-022
gb|BG645286.1|BG645286 EST506905 KV3 Medicago truncatula cD... 105 7e-022
gb|BF649665.1|BF649665 NF081H11EC1F1094 Elicited cell cultu... 102 9e-021
>gb|CA919619.1|CA919619 EST637337 MTUS Medicago truncatula cDNA clone MTUS-15H1, mRNA
sequence
Length = 606
Score = 184 bits (396), Expect = 2e-045
Identities = 81/141 (57%), Positives = 100/141 (70%)
Frame = -1 / -2
Query: 751 MYFDXXLPXXXXXXSDGNAGSSALXXXTCLQXXSRRIXYGKFXXXAKFQESPEAYTPAII 572
+YF +P DGN GSSA+ CLQ S+RI YGKF AKFQ +P++Y AII
Sbjct: 602 VYFRVLIPQLVKEGDDGNYGSSAVCDVMCLQALSKRIHYGKFVAEAKFQAAPDSYKAAII 423
Query: 571 AQDRDQLMNLLTXXTVERAVEHRVEAKAKIFGQEVNIGAKDKGSPPVYKIRPSLVAELYS 392
AQD+D+LM LLT VE +++ RV KAK +GQEV + KD+ + PVYKI PSLVA+LYS
Sbjct: 422 AQDKDRLMELLTYPEVEESIKRRVAVKAKTYGQEVAVNLKDQKTEPVYKINPSLVADLYS 243
Query: 391 YRIMPLTKEVEVAYLLRRLD* 329
IMPLTKEV+VAYLLR+LD*
Sbjct: 242 DWIMPLTKEVQVAYLLRKLD* 180
>gb|BM780235.1|BM780235 EST590811 KV2 Medicago truncatula cDNA clone pKV2-54M6, mRNA
sequence
Length = 818
Score = 100 bits (212), Expect(3) = 3e-043
Identities = 41/74 (55%), Positives = 53/74 (71%)
Frame = -1 / -2
Query: 667 CLQXXSRRIXYGKFXXXAKFQESPEAYTPAIIAQDRDQLMNLLTXXTVERAVEHRVEAKA 488
CLQ S+RI YGKF AKFQ +P++Y AIIAQD+D+LM LLT VE +++ RV KA
Sbjct: 538 CLQALSKRIHYGKFVAEAKFQAAPDSYKAAIIAQDKDRLMELLTYPEVEESIKRRVAVKA 359
Query: 487 KIFGQEVNIGAKDK 446
K +GQEV + KD+
Sbjct: 358 KTYGQEVAVNLKDQ 317
Score = 70.7 bits (148), Expect(3) = 3e-043
Identities = 32/42 (76%), Positives = 36/42 (85%)
Frame = -1 / -3
Query: 454 KDKGSPPVYKIRPSLVAELYSYRIMPLTKEVEVAYLLRRLD* 329
K K + PVYKI PSLVA+LYS IMPLTKEV+VAYLLR+LD*
Sbjct: 324 KIKKTEPVYKINPSLVADLYSDWIMPLTKEVQVAYLLRKLD* 199
Score = 44.6 bits (91), Expect(3) = 3e-043
Identities = 19/43 (44%), Positives = 21/43 (48%)
Frame = -1 / -1
Query: 811 YXXXXHPXAXSINIXXXXWKMYFDXXLPXXXXXXSDGNAGSSA 683
Y HP A SINI W +YF +P DGN GSSA
Sbjct: 683 YPQVLHPIAESININDNVWSVYFRVLIPQLVKEGDDGNYGSSA 555
>gb|CA923174.1|CA923174 EST640892 MTUS Medicago truncatula cDNA clone MTUS-64A11, mRNA
sequence
Length = 704
Score = 136 bits (292), Expect = 4e-031
Identities = 62/112 (55%), Positives = 75/112 (66%)
Frame = -1 / -3
Query: 664 LQXXSRRIXYGKFXXXAKFQESPEAYTPAIIAQDRDQLMNLLTXXTVERAVEHRVEAKAK 485
LQ S+RI YGKF AKFQE P Y AI A+DR +L+ LLT +VE +V+ RVE KA+
Sbjct: 585 LQALSKRIHYGKFVAEAKFQECPSVYEAAIKAKDRKRLLELLTYESVEESVQKRVEMKAR 406
Query: 484 IFGQEVNIGAKDKGSPPVYKIRPSLVAELYSYRIMPLTKEVEVAYLLRRLD* 329
F QEV I + PVY+I+ L+ LY IMPLTKEV+V YLLRRLD*
Sbjct: 405 TFSQEVKINEAGDVADPVYRIKSGLIGNLYGKWIMPLTKEVQVEYLLRRLD* 250
>gb|CA923195.1|CA923195 EST640913 MTUS Medicago truncatula cDNA clone MTUS-64D12, mRNA
sequence
Length = 803
Score = 136 bits (292), Expect = 4e-031
Identities = 62/112 (55%), Positives = 75/112 (66%)
Frame = -1 / -2
Query: 664 LQXXSRRIXYGKFXXXAKFQESPEAYTPAIIAQDRDQLMNLLTXXTVERAVEHRVEAKAK 485
LQ S+RI YGKF AKFQE P Y AI A+DR +L+ LLT +VE +V+ RVE KA+
Sbjct: 586 LQALSKRIHYGKFVAEAKFQECPSVYEAAIKAKDRKRLLELLTYESVEESVQKRVEMKAR 407
Query: 484 IFGQEVNIGAKDKGSPPVYKIRPSLVAELYSYRIMPLTKEVEVAYLLRRLD* 329
F QEV I + PVY+I+ L+ LY IMPLTKEV+V YLLRRLD*
Sbjct: 406 TFSQEVKINEAGDVADPVYRIKSGLIGNLYGKWIMPLTKEVQVEYLLRRLD* 251
>gb|AW774625.1|AW774625 EST333776 KV3 Medicago truncatula cDNA clone pKV3-23M13, mRNA
sequence
Length = 648
Score = 127 bits (271), Expect = 3e-028
Identities = 52/116 (44%), Positives = 70/116 (60%)
Frame = -1 / +1
Query: 796 HPXAXSINIXXXXWKMYFDXXLPXXXXXXSDGNAGSSALXXXTCLQXXSRRIXYGKFXXX 617
HP A SINI WKMYF LP DGN +A + LQ S+R+ YGKF
Sbjct: 223 HPGAASININKSLWKMYFHDLLPLFVASGDDGNYAQTAASDLSLLQAISKRVHYGKFVAE 402
Query: 616 AKFQESPEAYTPAIIAQDRDQLMNLLTXXTVERAVEHRVEAKAKIFGQEVNIGAKD 449
KF+ESP+ Y P I ++D++ LM LLT +VE V+ RVE KA +FGQ++++ + D
Sbjct: 403 VKFRESPQDYEPLIRSKDKEGLMKLLTFKSVEEMVKKRVEKKATLFGQDISLNSSD 570
>gb|BI273174.1|BI273174 NF091B12FL1F1096 Developing flower Medicago truncatula cDNA clone
NF091B12FL 5', mRNA sequence
Length = 696
Score = 127 bits (271), Expect = 3e-028
Identities = 52/116 (44%), Positives = 70/116 (60%)
Frame = -1 / +2
Query: 796 HPXAXSINIXXXXWKMYFDXXLPXXXXXXSDGNAGSSALXXXTCLQXXSRRIXYGKFXXX 617
HP A SINI WKMYF LP DGN +A + LQ S+R+ YGKF
Sbjct: 173 HPGAASININKSLWKMYFHDLLPLFVASGDDGNYAQTAASDLSLLQAISKRVHYGKFVAE 352
Query: 616 AKFQESPEAYTPAIIAQDRDQLMNLLTXXTVERAVEHRVEAKAKIFGQEVNIGAKD 449
KF+ESP+ Y P I ++D++ LM LLT +VE V+ RVE KA +FGQ++++ + D
Sbjct: 353 VKFRESPQDYEPLIRSKDKEGLMKLLTFKSVEEMVKKRVEKKATLFGQDISLNSSD 520
>gb|CX532800.1|CX532800 s13dNF71G05MJ036_271883 Methyl Jasmonate-Elicited Root Cell
Suspension Culture Medicago truncatula cDNA, mRNA
sequence
Length = 666
Score = 127 bits (271), Expect = 3e-028
Identities = 52/116 (44%), Positives = 70/116 (60%)
Frame = -1 / +3
Query: 796 HPXAXSINIXXXXWKMYFDXXLPXXXXXXSDGNAGSSALXXXTCLQXXSRRIXYGKFXXX 617
HP A SINI WKMYF LP DGN +A + LQ S+R+ YGKF
Sbjct: 267 HPGAASININKSLWKMYFHDLLPLFVASGDDGNYAQTAASDLSLLQAISKRVHYGKFVAE 446
Query: 616 AKFQESPEAYTPAIIAQDRDQLMNLLTXXTVERAVEHRVEAKAKIFGQEVNIGAKD 449
KF+ESP+ Y P I ++D++ LM LLT +VE V+ RVE KA +FGQ++++ + D
Sbjct: 447 VKFRESPQDYEPLIRSKDKEGLMKLLTFKSVEEMVKKRVEKKATLFGQDISLNSSD 614
>gb|AC147482.2| Medicago truncatula chromosome 7 BAC clone mth2-93e11, complete sequence
Length = 119127
Score = 122 bits (262), Expect = 6e-027
Identities = 55/94 (58%), Positives = 70/94 (74%)
Frame = -1 / -3
Query: 610 FQESPEAYTPAIIAQDRDQLMNLLTXXTVERAVEHRVEAKAKIFGQEVNIGAKDKGSPPV 431
F +S + A+I QD+D+LM LLT VE +++ RV KAK +GQEV + KD+ + PV
Sbjct: 117496 FAKSTQLSFTAMILQDKDRLMELLTYPEVEESIKRRVAVKAKTYGQEVAVNLKDQKTEPV 117317
Query: 430 YKIRPSLVAELYSYRIMPLTKEVEVAYLLRRLD* 329
YKI PSLVA+LYS IMPLTKEV+VAYLLR+LD*
Sbjct: 117316 YKINPSLVADLYSDWIMPLTKEVQVAYLLRKLD* 117215
>gb|AW775842.1|AW775842 EST334907 DSIL Medicago truncatula cDNA clone pDSIL-3E8, mRNA
sequence
Length = 654
Score = 110 bits (234), Expect = 4e-023
Identities = 47/102 (46%), Positives = 59/102 (57%)
Frame = -1 / +1
Query: 796 HPXAXSINIXXXXWKMYFDXXLPXXXXXXSDGNAGSSALXXXTCLQXXSRRIXYGKFXXX 617
HP A SINI WKMYF LP DGN +A + LQ S+R+ YGKF
Sbjct: 349 HPGAASININKSLWKMYFHDLLPLFVASGDDGNYAQTAASDLSLLQAISKRVHYGKFVAE 528
Query: 616 AKFQESPEAYTPAIIAQDRDQLMNLLTXXTVERAVEHRVEAK 491
KF+ESP+ Y P I ++D++ LM LLT +VE V+ RVE K
Sbjct: 529 VKFRESPQDYEPLIRSKDKEGLMKLLTFKSVEEMVKKRVEKK 654
>gb|BI309356.1|BI309356 EST530766 GPOD Medicago truncatula cDNA clone pGPOD-11O18 5' end,
mRNA sequence
Length = 567
Score = 109 bits (233), Expect = 6e-023
Identities = 47/92 (51%), Positives = 56/92 (60%)
Frame = -1 / +3
Query: 811 YXXXXHPXAXSINIXXXXWKMYFDXXLPXXXXXXSDGNAGSSALXXXTCLQXXSRRIXYG 632
Y HP A SINI W +YF +P DGN GSSA+ CLQ S+RI YG
Sbjct: 285 YPQVLHPIAESININDNVWSVYFRVLIPQLVKEGDDGNYGSSAVCDVMCLQALSKRIHYG 464
Query: 631 KFXXXAKFQESPEAYTPAIIAQDRDQLMNLLT 536
KF AKFQ +P++Y AIIAQD+D+LM LLT
Sbjct: 465 KFVAEAKFQAAPDSYKAAIIAQDKDRLMELLT 560
>gb|AC128660.31| Medicago truncatula clone mth2-14p23, complete sequence
Length = 99170
Score = 47.8 bits (98), Expect(4) = 6e-022
Identities = 20/33 (60%), Positives = 26/33 (78%)
Frame = -1 / +1
Query: 427 KIRPSLVAELYSYRIMPLTKEVEVAYLLRRLD* 329
K PS+ ++LY ++PLTKEV+V YLLRRLD*
Sbjct: 61252 KFDPSVASKLYEKWVIPLTKEVQVEYLLRRLD* 61350
Score = 47.8 bits (98), Expect(4) = 6e-022
Identities = 20/46 (43%), Positives = 22/46 (47%)
Frame = -1 / +1
Query: 796 HPXAXSINIXXXXWKMYFDXXLPXXXXXXSDGNAGSSALXXXTCLQ 659
HP A SINI WKMYF LP DGN +A + LQ
Sbjct: 59722 HPGAASININKSLWKMYFHDLLPLFVASGDDGNYAQTAASDLSLLQ 59859
Score = 46.9 bits (96), Expect(4) = 6e-022
Identities = 18/39 (46%), Positives = 28/39 (71%)
Frame = -1 / +1
Query: 565 DRDQLMNLLTXXTVERAVEHRVEAKAKIFGQEVNIGAKD 449
D++ LM LLT +VE V+ RVE KA +FGQ++++ + D
Sbjct: 61117 DKEGLMKLLTFKSVEEMVKKRVEKKATLFGQDISLNSSD 61233
Score = 42.8 bits (87), Expect(4) = 6e-022
Identities = 16/30 (53%), Positives = 20/30 (66%)
Frame = -1 / +1
Query: 664 LQXXSRRIXYGKFXXXAKFQESPEAYTPAI 575
LQ S+R+ YGKF KF+ESP+ Y P I
Sbjct: 60223 LQAISKRVHYGKFVAEVKFRESPQDYEPLI 60312
>gb|BG645286.1|BG645286 EST506905 KV3 Medicago truncatula cDNA clone pKV3-39D20 5' end,
mRNA sequence
Length = 749
Score = 105 bits (225), Expect = 7e-022
Identities = 45/92 (48%), Positives = 55/92 (59%)
Frame = -1 / +3
Query: 811 YXXXXHPXAXSINIXXXXWKMYFDXXLPXXXXXXSDGNAGSSALXXXTCLQXXSRRIXYG 632
Y HP A SINI W +YF +P DGN GS A+ CLQ S+RI YG
Sbjct: 474 YPQVLHPIAESININDNVWSVYFRVLIPQLVKEGDDGNYGSFAVCDVMCLQALSKRIHYG 653
Query: 631 KFXXXAKFQESPEAYTPAIIAQDRDQLMNLLT 536
KF AKFQ +P++Y AIIAQD+D+LM +LT
Sbjct: 654 KFGAEAKFQAAPDSYKAAIIAQDKDRLMEMLT 749
>gb|BF649665.1|BF649665 NF081H11EC1F1094 Elicited cell culture Medicago truncatula cDNA
clone NF081H11EC 5', mRNA sequence
Length = 605
Score = 102 bits (217), Expect = 9e-021
Identities = 43/96 (44%), Positives = 55/96 (57%)
Frame = -1 / +2
Query: 796 HPXAXSINIXXXXWKMYFDXXLPXXXXXXSDGNAGSSALXXXTCLQXXSRRIXYGKFXXX 617
HP A SINI WKMYF LP DGN +A + LQ S+R+ YGKF
Sbjct: 314 HPGAASININKSLWKMYFHDLLPLFVASGDDGNYAQTAASDLSLLQAISKRVHYGKFVAE 493
Query: 616 AKFQESPEAYTPAIIAQDRDQLMNLLTXXTVERAVE 509
KF+ESP+ Y P I ++D++ LM LLT +VE V+
Sbjct: 494 VKFRESPQDYEPLIRSKDKEXLMKLLTFKSVEXMVK 601
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 699,569,662
Number of Sequences: 392609
Number of extensions: 9462444
Number of successful extensions: 34524
Number of sequences better than 1.0e-020: 16
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 28737905460
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)