TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2493528.2.1
(627 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ124680.1|BQ124680 EST610256 GLSD Medicago truncatula c... 291 7e-078
gb|BI307906.1|BI307906 EST529316 GPOD Medicago truncatula c... 223 2e-057
gb|BE319904.2|BE319904 NF023C03RT1F1018 Developing root Med... 139 3e-032
>gb|BQ124680.1|BQ124680 EST610256 GLSD Medicago truncatula cDNA clone pGLSD-35P6, mRNA
sequence
Length = 632
Score = 291 bits (630), Expect = 7e-078
Identities = 127/165 (76%), Positives = 146/165 (88%)
Frame = -2 / +2
Query: 599 EDRSLLLEKEYRNNILDDLLEVKSFLTQRLGEMRNADTSSLQHQVQAVSPFVLQQHAPDS 420
E RS LL+ EYRN ILDDL E+KSFL QRL E++N +T SLQ+QVQAVSPFVLQQ+A D+
Sbjct: 14 E*RSQLLDTEYRNKILDDLYEIKSFLNQRLAELKNEETLSLQNQVQAVSPFVLQQYAADT 193
Query: 419 LENMLVEISSAISLLTNQKTLDLIMILNSKRFLDRLVSSLEEKKHHEVKLREGLGDLSVK 240
+E M +IS AISLLTN+KT DLIMILNSKRFLDRLV+SLEEKKHHEVKL++GL DLS K
Sbjct: 194 IETMQSDISLAISLLTNRKTRDLIMILNSKRFLDRLVNSLEEKKHHEVKLKDGLKDLSTK 373
Query: 239 RMELQNALSSSWPKQEAAITKTRELKKLCESTLSSVFDGRPVYII 105
RMELQN+LSS WPKQ+AA+TKT+ELKKLCESTLSS+FDGRPV II
Sbjct: 374 RMELQNSLSSLWPKQDAAVTKTKELKKLCESTLSSMFDGRPVNII 508
Score = 175 bits (376), Expect = 7e-043
Identities = 89/165 (53%), Positives = 99/165 (60%)
Frame = +1 / -3
Query: 106 IIYTGLPSKTXXXXXXXXXXXXXXXXIAASCFGHDDDSAFCSSIRLTDKSPKPXXXXXXX 285
II TGLPS AASCFGH++D+ FCSS+RL DKS P
Sbjct: 507 IILTGLPSNMDESVLSHSFFSSFVFVTAASCFGHNEDNEFCSSMRLVDKSFNPSFNLTS* 328
Query: 286 XXXXXKLETNLSRNLLELRIIIRSRVF*LVSNEIADEISTSMFSKLSGACCWRTKGETAC 465
K T+LSRNLLELRIIIRS VF VSNEIA EIS + S +S A C TKGETAC
Sbjct: 327 CFFSSKEFTSLSRNLLELRIIIRSLVFLFVSNEIAKEISLCIVSIVSAAYCCSTKGETAC 148
Query: 466 T*CCRDDVSAFLISPKR*VKNDFTSSKSSRILFLYSFSSNKLLSS 600
T* C D VS+F S R* KND S +SSRILFLYS S + L S
Sbjct: 147 T*FCSDKVSSFFNSANR*FKNDLISYRSSRILFLYSVSKSWLRHS 13
>gb|BI307906.1|BI307906 EST529316 GPOD Medicago truncatula cDNA clone pGPOD-1E14 5' end,
mRNA sequence
Length = 639
Score = 223 bits (482), Expect = 2e-057
Identities = 96/123 (78%), Positives = 112/123 (91%)
Frame = -2 / -1
Query: 440 QQHAPDSLENMLVEISSAISLLTNQKTLDLIMILNSKRFLDRLVSSLEEKKHHEVKLREG 261
+Q+A D++E M +IS AISLLTN+KT DLIMILNSKRFLDRLV+SLEEKKHHEVKL++G
Sbjct: 633 RQYAADTIETMQSDISLAISLLTNRKTRDLIMILNSKRFLDRLVNSLEEKKHHEVKLKDG 454
Query: 260 LGDLSVKRMELQNALSSSWPKQEAAITKTRELKKLCESTLSSVFDGRPVYIIGEINTLLS 81
L DLS KRMELQN+LSS WPKQ+AA+TKT+ELKKLCESTLSS+FDGRPV IIGEINTLL+
Sbjct: 453 LKDLSTKRMELQNSLSSLWPKQDAAVTKTKELKKLCESTLSSMFDGRPVNIIGEINTLLT 274
Query: 80 SSV 72
S +
Sbjct: 273 SGL 265
Score = 122 bits (262), Expect = 4e-027
Identities = 61/119 (51%), Positives = 71/119 (59%)
Frame = +1 / +2
Query: 82 LSKVLISPIIYTGLPSKTXXXXXXXXXXXXXXXXIAASCFGHDDDSAFCSSIRLTDKSPK 261
+++VLISPII TGLPS AASCFGH++D+ FCSS+RL DKS
Sbjct: 275 VNRVLISPIILTGLPSNMDESVLSHSFFSSFVFVTAASCFGHNEDNEFCSSMRLVDKSFN 454
Query: 262 PXXXXXXXXXXXXKLETNLSRNLLELRIIIRSRVF*LVSNEIADEISTSMFSKLSGACC 438
P K T+LSRNLLELRIIIRS VF VSNEIA EIS + S +S A C
Sbjct: 455 PSFNLTS*CFFSSKEFTSLSRNLLELRIIIRSLVFLFVSNEIAKEISLCIVSIVSAAYC 631
>gb|BE319904.2|BE319904 NF023C03RT1F1018 Developing root Medicago truncatula cDNA clone
NF023C03RT 5', mRNA sequence
Length = 528
Score = 139 bits (299), Expect = 3e-032
Identities = 62/91 (68%), Positives = 75/91 (82%)
Frame = -2 / +2
Query: 599 EDRSLLLEKEYRNNILDDLLEVKSFLTQRLGEMRNADTSSLQHQVQAVSPFVLQQHAPDS 420
E RS LL+ EYRN ILDDL E+KSFL QRL E++N +T SLQ+QVQAVSPFVLQQ+A D+
Sbjct: 224 EGRSQLLDTEYRNKILDDLYEIKSFLNQRLAELKNEETLSLQNQVQAVSPFVLQQYAADT 403
Query: 419 LENMLVEISSAISLLTNQKTLDLIMILNSKR 327
+E M +IS AISLLTN+KT DLIMILN ++
Sbjct: 404 IETMQSDISLAISLLTNRKTRDLIMILNLQK 496
Score = 110 bits (235), Expect = 2e-023
Identities = 56/88 (63%), Positives = 61/88 (69%)
Frame = +1 / -1
Query: 337 LRIIIRSRVF*LVSNEIADEISTSMFSKLSGACCWRTKGETACT*CCRDDVSAFLISPKR 516
LRIIIRS VF VSNEIA EIS + S +S A C TKGETACT* C D VS+F S R
Sbjct: 486 LRIIIRSLVFLFVSNEIAKEISLCIVSIVSAAYCCSTKGETACT*FCSDKVSSFFNSANR 307
Query: 517 *VKNDFTSSKSSRILFLYSFSSNKLLSS 600
* KND S +SSRILFLYS S + LL S
Sbjct: 306 *FKNDLISYRSSRILFLYSVSKSWLLPS 223
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 587,146,195
Number of Sequences: 392609
Number of extensions: 7302446
Number of successful extensions: 38712
Number of sequences better than 1.0e-020: 3
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 19410690530
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)