TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2441276.2.1
(1796 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AC158497.2| Medicago truncatula chromosome 2 BAC clone m... 66 1e-028
gb|CX527554.1|CX527554 s13dNF43H03AT032_515620 Aphid-Infect... 55 1e-022
gb|AW689134.1|AW689134 NF015G05ST1F1000 Developing stem Med... 51 8e-022
gb|CX534173.1|CX534173 s13dNF0TC01MJ002_322105 Methyl Jasmo... 64 7e-021
>gb|AC158497.2| Medicago truncatula chromosome 2 BAC clone mth2-48e18, complete sequence
Length = 146321
Score = 66.1 bits (138), Expect(7) = 1e-028
Identities = 27/57 (47%), Positives = 40/57 (70%)
Frame = -2 / -2
Query: 1789 LRGDNGELRLGVRRAAQLKNGSAFPALYNQCSNLGSLPNVAHAVATKSVFHIYYNPR 1619
+RG++GELRLG+RRA QLK+ +F L + GSL +V +A++ +S F + YNPR
Sbjct: 139375 IRGEDGELRLGIRRAVQLKSSGSFGGLSGMQLDPGSLMDVVNALSKRSAFSVCYNPR 139205
Score = 46.0 bits (94), Expect(7) = 1e-028
Identities = 21/37 (56%), Positives = 26/37 (70%)
Frame = -3 / -3
Query: 1074 QELVSEHHSEALGFPTVAQALGSLQDSKRSCKVKKYF 964
Q+ +SE L FP +A AL +L DSKRSCKVKK+F
Sbjct: 137892 QQEISECTQRLLIFPLMA*ALVNLSDSKRSCKVKKFF 137782
Score = 44.1 bits (90), Expect(7) = 1e-028
Identities = 18/31 (58%), Positives = 20/31 (64%)
Frame = -2 / -1
Query: 1432 LMVRWDDDVDFRQPNRISPWEIELTSSVSGS 1340
L VRWDD R NR+SPWEIE + S S S
Sbjct: 138323 LQVRWDDIEASRHNNRVSPWEIEPSGSASNS 138231
Score = 41.4 bits (84), Expect(7) = 1e-028
Identities = 16/28 (57%), Positives = 20/28 (71%)
Frame = -2 / -2
Query: 1267 NGSGRPDFAESAQFHKVLQGQELLGYRT 1184
+G G DF ES +F KVLQGQE++G T
Sbjct: 138061 DGIGASDFGESLRFRKVLQGQEIMGMNT 137978
Score = 32.7 bits (65), Expect(7) = 1e-028
Identities = 11/18 (61%), Positives = 17/18 (94%)
Frame = -2 / -1
Query: 1552 AGSRFKVRYESDDASERR 1499
AG RF++R+E++DA+ERR
Sbjct: 139034 AGMRFRMRFETEDAAERR 138981
Score = 29.9 bits (59), Expect(7) = 1e-028
Identities = 9/11 (81%), Positives = 10/11 (90%)
Frame = -2 / -1
Query: 1456 WRGSKWKCLMV 1424
W GSKWKCL+V
Sbjct: 138854 WPGSKWKCLLV 138822
Score = 29.5 bits (58), Expect(7) = 1e-028
Identities = 11/15 (73%), Positives = 13/15 (86%)
Frame = -2 / -1
Query: 1501 RCTGIIAGIGDADPM 1457
R TG+IAGI DADP+
Sbjct: 138902 RFTGLIAGISDADPV 138858
>gb|CX527554.1|CX527554 s13dNF43H03AT032_515620 Aphid-Infected Shoots Medicago truncatula
cDNA, mRNA sequence
Length = 610
Score = 54.7 bits (113), Expect(3) = 1e-022
Identities = 17/31 (54%), Positives = 23/31 (74%)
Frame = -2 / +2
Query: 1456 WRGSKWKCLMVRWDDDVDFRQPNRISPWEIE 1364
W SKW+CL VRWD+ + +P R+SPW+IE
Sbjct: 329 WPTSKWRCLKVRWDETSNIPRPERVSPWKIE 421
Score = 49.6 bits (102), Expect(3) = 1e-022
Identities = 22/54 (40%), Positives = 31/54 (57%)
Frame = -2 / +2
Query: 1774 GELRLGVRRAAQLKNGSAFPALYNQCSNLGSLPNVAHAVATKSVFHIYYNPRLS 1613
GELR+GVRRA + + + + +LG L HAV T ++F +YY PR S
Sbjct: 8 GELRVGVRRAMRQQGNVPSSVISSHSMHLGVLATAWHAVLTGTMFTVYYKPRTS 169
Score = 42.3 bits (86), Expect(3) = 1e-022
Identities = 16/29 (55%), Positives = 22/29 (75%)
Frame = -2 / +2
Query: 1549 GSRFKVRYESDDASERRCTGIIAGIGDAD 1463
G RFK+R+E ++A E+R TG I GI D+D
Sbjct: 233 GMRFKMRFEGEEAPEQRFTGTIVGIEDSD 319
>gb|AW689134.1|AW689134 NF015G05ST1F1000 Developing stem Medicago truncatula cDNA clone
NF015G05ST 5', mRNA sequence
Length = 623
Score = 50.6 bits (104), Expect(4) = 8e-022
Identities = 15/29 (51%), Positives = 21/29 (72%)
Frame = -2 / +3
Query: 1456 WRGSKWKCLMVRWDDDVDFRQPNRISPWE 1370
W SKW+CL VRWD+ + +P R+SPW+
Sbjct: 423 WPTSKWRCLKVRWDETSNIPRPERVSPWK 509
Score = 47.3 bits (97), Expect(4) = 8e-022
Identities = 21/54 (38%), Positives = 30/54 (55%)
Frame = -2 / +3
Query: 1774 GELRLGVRRAAQLKNGSAFPALYNQCSNLGSLPNVAHAVATKSVFHIYYNPRLS 1613
G LR+GVRRA + + + + +LG L HAV T ++F +YY PR S
Sbjct: 102 GSLRVGVRRAMRQQGNVPSSVISSHSMHLGVLATAWHAVLTGTMFTVYYKPRTS 263
Score = 40.9 bits (83), Expect(4) = 8e-022
Identities = 16/29 (55%), Positives = 21/29 (72%)
Frame = -2 / +3
Query: 1549 GSRFKVRYESDDASERRCTGIIAGIGDAD 1463
G RFK+R+E + A E+R TG I GI D+D
Sbjct: 327 GMRFKMRFEGEXAPEQRFTGTIVGIEDSD 413
Score = 22.1 bits (42), Expect(4) = 8e-022
Identities = 7/9 (77%), Positives = 9/9 (100%)
Frame = -2 / +2
Query: 1795 LFLRGDNGE 1769
+FLRG+NGE
Sbjct: 80 IFLRGENGE 106
>gb|CX534173.1|CX534173 s13dNF0TC01MJ002_322105 Methyl Jasmonate-Elicited Root Cell
Suspension Culture Medicago truncatula cDNA, mRNA
sequence
Length = 580
Score = 63.8 bits (133), Expect(3) = 7e-021
Identities = 27/61 (44%), Positives = 38/61 (62%)
Frame = -2 / +2
Query: 1795 LFLRGDNGELRLGVRRAAQLKNGSAFPALYNQCSNLGSLPNVAHAVATKSVFHIYYNPRL 1616
+FLRG+NGELR+GVRRA + + + + +LG L HAV T ++F +YY PR
Sbjct: 176 IFLRGENGELRVGVRRAMRQQGNVPSSVISSHSMHLGVLATAWHAVLTGTMFTVYYKPRT 355
Query: 1615 S 1613
S
Sbjct: 356 S 358
Score = 42.3 bits (86), Expect(3) = 7e-021
Identities = 16/29 (55%), Positives = 22/29 (75%)
Frame = -2 / +2
Query: 1549 GSRFKVRYESDDASERRCTGIIAGIGDAD 1463
G RFK+R+E ++A E+R TG I GI D+D
Sbjct: 422 GMRFKMRFEGEEAPEQRFTGTIVGIEDSD 508
Score = 34.5 bits (69), Expect(3) = 7e-021
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = -2 / +2
Query: 1456 WRGSKWKCLMVRWDD 1412
W SKW+CL VRWD+
Sbjct: 518 WPTSKWRCLKVRWDE 562
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 1,929,279,229
Number of Sequences: 392609
Number of extensions: 29481731
Number of successful extensions: 103400
Number of sequences better than 1.0e-020: 5
length of database: 147,244,331
effective HSP length: 57
effective length of database: 124,865,618
effective search space used: 67552299338
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)