TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2437739.2.1
(574 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AC148816.3| Medicago truncatula chromosome 7 BAC clone m... 207 1e-052
gb|BG452923.1|BG452923 NF086F12LF1F1096 Developing leaf Med... 202 3e-051
gb|AJ547914.1|AJ547914 AJ547914 MTAPHEU Medicago truncatula... 197 2e-049
gb|AJ548213.1|AJ548213 AJ548213 MTAPHEU Medicago truncatula... 197 2e-049
gb|CX523067.1|CX523067 s13dNF88C10VI082_471938 Virus-Infect... 193 3e-048
gb|AW774529.1|AW774529 EST333680 KV3 Medicago truncatula cD... 189 2e-047
gb|BQ143777.1|BQ143777 NF058A09DT1F1069 Drought Medicago tr... 187 1e-046
gb|CX539956.1|CX539956 s13dNF43B05GS041_463184 Germinating ... 186 3e-046
gb|AJ548018.1|AJ548018 AJ548018 MTAPHEU Medicago truncatula... 133 3e-030
emb|CT033766.1| Medicago truncatula chromosome 5 clone mth4... 83 2e-022
>gb|AC148816.3| Medicago truncatula chromosome 7 BAC clone mth2-16i24, complete
sequence
Length = 123655
Score = 207 bits (446), Expect = 1e-052
Identities = 76/105 (72%), Positives = 89/105 (84%)
Frame = +1 / -2
Query: 1 SMNLTIPEPGPSTIQTVCGAVPCATVLISGRPVVIQPFLGTVHALVAAWLPGSEGQGITD 180
+MNLTIP PGP I VCGA+ C ++ISGRP+VI+P++G + A+VA WLPGSEGQG+ D
Sbjct: 77085 NMNLTIPNPGPEIITNVCGAMKCVVIIISGRPLVIEPYVGLIDAVVAGWLPGSEGQGVAD 76906
Query: 181 VLFGDYGFTGTLPRTWFKSVDQLPMNYGDEHYDPLFPLGFGLTTQ 315
VLFGDYGFTG LPRTWFKSVDQLPMN GD HYDP+FP GFGLTT+
Sbjct: 76905 VLFGDYGFTGKLPRTWFKSVDQLPMNVGDPHYDPVFPFGFGLTTK 76771
Score = 107 bits (229), Expect = 1e-022
Identities = 50/85 (58%), Positives = 55/85 (64%)
Frame = -3 / +2
Query: 257 FIGSWSTDLNHVRGSVPVKP*SPKSTSVMPWPSLPGSQAATSACTVPRNGCITTGRPLMS 78
FIGS ST LNHV GS PVKP*SP TS P PSLPGSQ AT+A P G IT GRP +
Sbjct: 76829 FIGS*STLLNHVLGSFPVKP*SPNKTSATPCPSLPGSQPATTASIKPTYGSITKGRPEIM 77008
Query: 77 TVAQGTAPHTVWMVLGPGSGIVRFM 3
AP T M+ GPG GIV+F+
Sbjct: 77009 ITTHFIAPQTFVMISGPGFGIVKFI 77083
>gb|BG452923.1|BG452923 NF086F12LF1F1096 Developing leaf Medicago truncatula cDNA clone
NF086F12LF 5', mRNA sequence
Length = 655
Score = 202 bits (436), Expect = 3e-051
Identities = 76/104 (73%), Positives = 89/104 (85%)
Frame = +1 / +2
Query: 1 SMNLTIPEPGPSTIQTVCGAVPCATVLISGRPVVIQPFLGTVHALVAAWLPGSEGQGITD 180
S+NLTI EPGPSTI VCG++ C VL++GRPVVIQP+L + ALVAAWLPG+EGQG+ D
Sbjct: 116 SLNLTIAEPGPSTITNVCGSIQCVVVLVTGRPVVIQPYLSKIDALVAAWLPGTEGQGVAD 295
Query: 181 VLFGDYGFTGTLPRTWFKSVDQLPMNYGDEHYDPLFPLGFGLTT 312
VL+GD+ FTG L RTWFK+VDQLPMN GD+HYDPLFP GFGLTT
Sbjct: 296 VLYGDFEFTGKLARTWFKTVDQLPMNVGDKHYDPLFPFGFGLTT 427
Score = 119 bits (254), Expect = 4e-026
Identities = 53/84 (63%), Positives = 57/84 (67%)
Frame = -3 / -2
Query: 257 FIGSWSTDLNHVRGSVPVKP*SPKSTSVMPWPSLPGSQAATSACTVPRNGCITTGRPLMS 78
FIGSWST LNHV S+PV SP TS PWPS+PGS AATSA R G ITTGRP+
Sbjct: 372 FIGSWSTVLNHVLASLPVNSKSP*RTSATPWPSVPGSHAATSASIFDRYG*ITTGRPVTR 193
Query: 77 TVAQGTAPHTVWMVLGPGSGIVRF 6
T PHT+ MVLGPGS IVRF
Sbjct: 192 TTTH*IEPHTLVMVLGPGSAIVRF 121
>gb|AJ547914.1|AJ547914 AJ547914 MTAPHEU Medicago truncatula cDNA clone mtaehac110004b10,
mRNA sequence
Length = 573
Score = 197 bits (424), Expect = 2e-049
Identities = 76/105 (72%), Positives = 83/105 (79%)
Frame = +1 / -1
Query: 1 SMNLTIPEPGPSTIQTVCGAVPCATVLISGRPVVIQPFLGTVHALVAAWLPGSEGQGITD 180
S+NLTI G TI VCG V C VLISGRP+VIQP+L + LVA WLPGSEG G+ D
Sbjct: 420 SLNLTISGQGYGTINNVCGVVKCVVVLISGRPIVIQPYLEKIEGLVAGWLPGSEGSGVAD 241
Query: 181 VLFGDYGFTGTLPRTWFKSVDQLPMNYGDEHYDPLFPLGFGLTTQ 315
VLFGDYGFTG LPRTWFK+VDQLPMN GD HYDPLFP GFGLTT+
Sbjct: 240 VLFGDYGFTGKLPRTWFKTVDQLPMNVGDSHYDPLFPFGFGLTTE 106
Score = 105 bits (224), Expect = 6e-022
Identities = 48/84 (57%), Positives = 56/84 (66%)
Frame = -3 / +2
Query: 257 FIGSWSTDLNHVRGSVPVKP*SPKSTSVMPWPSLPGSQAATSACTVPRNGCITTGRPLMS 78
FIG+ ST LNHV GS+PV P*SP TS P PSLPGSQ ATS R GCIT G PL++
Sbjct: 164 FIGN*STVLNHVLGSLPVNP*SPNKTSATPEPSLPGSQPATSPSIFSRYGCITIGLPLIN 343
Query: 77 TVAQGTAPHTVWMVLGPGSGIVRF 6
T T PHT+++V P IV+F
Sbjct: 344 TTTHFTTPHTLFIVPYPCPEIVKF 415
>gb|AJ548213.1|AJ548213 AJ548213 MTAPHEU Medicago truncatula cDNA clone mtaehac110004b12,
mRNA sequence
Length = 545
Score = 197 bits (424), Expect = 2e-049
Identities = 76/105 (72%), Positives = 83/105 (79%)
Frame = +1 / -3
Query: 1 SMNLTIPEPGPSTIQTVCGAVPCATVLISGRPVVIQPFLGTVHALVAAWLPGSEGQGITD 180
S+NLTI G TI VCG V C VLISGRP+VIQP+L + LVA WLPGSEG G+ D
Sbjct: 420 SLNLTISGQGYGTINNVCGVVKCVVVLISGRPIVIQPYLEKIEGLVAGWLPGSEGSGVAD 241
Query: 181 VLFGDYGFTGTLPRTWFKSVDQLPMNYGDEHYDPLFPLGFGLTTQ 315
VLFGDYGFTG LPRTWFK+VDQLPMN GD HYDPLFP GFGLTT+
Sbjct: 240 VLFGDYGFTGKLPRTWFKTVDQLPMNVGDSHYDPLFPFGFGLTTE 106
Score = 105 bits (224), Expect = 6e-022
Identities = 48/84 (57%), Positives = 56/84 (66%)
Frame = -3 / +2
Query: 257 FIGSWSTDLNHVRGSVPVKP*SPKSTSVMPWPSLPGSQAATSACTVPRNGCITTGRPLMS 78
FIG+ ST LNHV GS+PV P*SP TS P PSLPGSQ ATS R GCIT G PL++
Sbjct: 164 FIGN*STVLNHVLGSLPVNP*SPNKTSATPEPSLPGSQPATSPSIFSRYGCITIGLPLIN 343
Query: 77 TVAQGTAPHTVWMVLGPGSGIVRF 6
T T PHT+++V P IV+F
Sbjct: 344 TTTHFTTPHTLFIVPYPCPEIVKF 415
>gb|CX523067.1|CX523067 s13dNF88C10VI082_471938 Virus-Infected Leaves Medicago truncatula
cDNA, mRNA sequence
Length = 546
Score = 193 bits (415), Expect = 3e-048
Identities = 72/98 (73%), Positives = 83/98 (84%)
Frame = +1 / +3
Query: 22 EPGPSTIQTVCGAVPCATVLISGRPVVIQPFLGTVHALVAAWLPGSEGQGITDVLFGDYG 201
+PGP+ I VCGAV C V ++GRPVVI+P+L ++ ALVAAWLPGSEGQG+ DVLFGDYG
Sbjct: 12 DPGPNIINNVCGAVKCVVVTVTGRPVVIEPYLSSIDALVAAWLPGSEGQGVADVLFGDYG 191
Query: 202 FTGTLPRTWFKSVDQLPMNYGDEHYDPLFPLGFGLTTQ 315
FTG L RTWFKSVDQLPMN GD HYDPLFP GFGLT++
Sbjct: 192 FTGKLARTWFKSVDQLPMNVGDPHYDPLFPYGFGLTSE 305
Score = 109 bits (233), Expect = 3e-023
Identities = 49/79 (62%), Positives = 55/79 (69%)
Frame = -3 / -3
Query: 257 FIGSWSTDLNHVRGSVPVKP*SPKSTSVMPWPSLPGSQAATSACTVPRNGCITTGRPLMS 78
FIGS STDLNHV S+PVKP*SPK TS P PS PG+ AAT+A R G ITTG P+
Sbjct: 247 FIGS*STDLNHVLASLPVKP*SPKRTSATPCPSDPGNHAATNASMEERYGSITTGLPVTV 68
Query: 77 TVAQGTAPHTVWMVLGPGS 21
T TAPHT+ M+ GPGS
Sbjct: 67 TTTHLTAPHTLLMIFGPGS 11
>gb|AW774529.1|AW774529 EST333680 KV3 Medicago truncatula cDNA clone pKV3-22J22, mRNA
sequence
Length = 450
Score = 189 bits (408), Expect = 2e-047
Identities = 69/99 (69%), Positives = 82/99 (82%)
Frame = +1 / -3
Query: 19 PEPGPSTIQTVCGAVPCATVLISGRPVVIQPFLGTVHALVAAWLPGSEGQGITDVLFGDY 198
P PG I VCGA+ C ++ISGRP+VI+P++G + A+VA WLPGSEGQG+ DVLFG+Y
Sbjct: 448 PNPGREIITNVCGAMKCVVIIISGRPLVIEPYVGLIDAVVAGWLPGSEGQGVADVLFGEY 269
Query: 199 GFTGTLPRTWFKSVDQLPMNYGDEHYDPLFPLGFGLTTQ 315
GFTG LPRTWFKSVDQLPMN GD HYDP+FP GFGLTT+
Sbjct: 268 GFTGKLPRTWFKSVDQLPMNVGDPHYDPVFPFGFGLTTK 152
>gb|BQ143777.1|BQ143777 NF058A09DT1F1069 Drought Medicago truncatula cDNA clone NF058A09DT
5', mRNA sequence
Length = 859
Score = 187 bits (403), Expect = 1e-046
Identities = 72/104 (69%), Positives = 81/104 (77%)
Frame = +1 / +2
Query: 1 SMNLTIPEPGPSTIQTVCGAVPCATVLISGRPVVIQPFLGTVHALVAAWLPGSEGQGITD 180
S+NLTI G TI VCG V C VL++GRPV IQP+L + LVAAWLPGSEG G+ D
Sbjct: 158 SLNLTISGNGTETINNVCGRVKCVVVLVTGRPVAIQPYLNKIDGLVAAWLPGSEGTGVAD 337
Query: 181 VLFGDYGFTGTLPRTWFKSVDQLPMNYGDEHYDPLFPLGFGLTT 312
VLFGDYGFTG L RTWFK+VDQLPMN GD HYDPLFP GFGL++
Sbjct: 338 VLFGDYGFTGKLARTWFKTVDQLPMNVGDSHYDPLFPFGFGLSS 469
>gb|CX539956.1|CX539956 s13dNF43B05GS041_463184 Germinating Seed Medicago truncatula cDNA,
mRNA sequence
Length = 601
Score = 186 bits (400), Expect = 3e-046
Identities = 69/95 (72%), Positives = 81/95 (85%)
Frame = +1 / +1
Query: 28 GPSTIQTVCGAVPCATVLISGRPVVIQPFLGTVHALVAAWLPGSEGQGITDVLFGDYGFT 207
GPSTI VCG++ C VL++GRPVVIQP+L + ALVAAWLPG+EGQG+ DVL+GD+ FT
Sbjct: 1 GPSTITNVCGSIQCVVVLVTGRPVVIQPYLSKIDALVAAWLPGTEGQGVADVLYGDFEFT 180
Query: 208 GTLPRTWFKSVDQLPMNYGDEHYDPLFPLGFGLTT 312
G L RTWFK+VDQLPMN GD+HYDPLFP GFGLTT
Sbjct: 181 GKLARTWFKTVDQLPMNVGDKHYDPLFPFGFGLTT 285
Score = 102 bits (218), Expect = 4e-021
Identities = 46/76 (60%), Positives = 50/76 (65%)
Frame = -3 / -3
Query: 257 FIGSWSTDLNHVRGSVPVKP*SPKSTSVMPWPSLPGSQAATSACTVPRNGCITTGRPLMS 78
FIGSWST LNHV S+PV SP TS PWPS+PGS AATSA R G ITTGRP+
Sbjct: 230 FIGSWSTVLNHVLASLPVNSKSP*RTSATPWPSVPGSHAATSASIFDRYG*ITTGRPVTR 51
Query: 77 TVAQGTAPHTVWMVLG 30
T PHT+ MVLG
Sbjct: 50 TTTH*IEPHTLVMVLG 3
>gb|AJ548018.1|AJ548018 AJ548018 MTAPHEU Medicago truncatula cDNA clone mtaehac110003b06,
mRNA sequence
Length = 352
Score = 133 bits (284), Expect = 3e-030
Identities = 49/63 (77%), Positives = 56/63 (88%)
Frame = +1 / -1
Query: 124 VHALVAAWLPGSEGQGITDVLFGDYGFTGTLPRTWFKSVDQLPMNYGDEHYDPLFPLGFG 303
+ ALVAAWLPG+EGQG+ DVL+GD+ FTG L RTWFK+VDQLPMN GD+HYDPLFP GFG
Sbjct: 349 IDALVAAWLPGTEGQGVADVLYGDFEFTGKLARTWFKTVDQLPMNVGDKHYDPLFPFGFG 170
Query: 304 LTT 312
LTT
Sbjct: 169 LTT 161
>emb|CT033766.1| Medicago truncatula chromosome 5 clone mth4-42c9, WORKING DRAFT SEQUENCE
Length = 235225
Score = 82.6 bits (174), Expect(3) = 2e-022
Identities = 32/51 (62%), Positives = 39/51 (76%)
Frame = +1 / +3
Query: 97 VVIQPFLGTVHALVAAWLPGSEGQGITDVLFGDYGFTGTLPRTWFKSVDQL 249
V+ Q L ALVAAWLPGSEG+GITDV+FGD+ F G LP TWF+ ++QL
Sbjct: 149559 VLEQSLLEKTEALVAAWLPGSEGKGITDVIFGDHDFKGKLPMTWFRRIEQL 149711
Score = 82.2 bits (173), Expect(3) = 3e-022
Identities = 32/51 (62%), Positives = 39/51 (76%)
Frame = +1 / -1
Query: 97 VVIQPFLGTVHALVAAWLPGSEGQGITDVLFGDYGFTGTLPRTWFKSVDQL 249
V+ Q L ALVAAWLPGSEG+GITDV+FGD+ F G LP TWF+ ++QL
Sbjct: 116587 VLEQGLLEKTEALVAAWLPGSEGKGITDVIFGDHDFKGKLPMTWFRRIEQL 116435
Score = 34.1 bits (68), Expect(3) = 2e-022
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = +1 / +3
Query: 10 LTIPEPGPSTIQTVCGAVPCATVLISGRPVVIQPFL 117
L IP G + V +P +LISGRP+V++ L
Sbjct: 149469 LVIPFNGAGIVDIVSDKIPTLVILISGRPLVLEQSL 149576
Score = 33.6 bits (67), Expect(3) = 3e-022
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = +1 / -1
Query: 10 LTIPEPGPSTIQTVCGAVPCATVLISGRPVVIQ 108
L IP G + + +P +LISGRP+V++
Sbjct: 116677 LVIPFNGAGIVDIIADKIPTLVILISGRPLVLE 116579
Score = 27.2 bits (53), Expect(3) = 2e-022
Identities = 9/10 (90%), Positives = 10/10 (100%)
Frame = +1 / +3
Query: 277 DPLFPLGFGL 306
DPLFPLG+GL
Sbjct: 149745 DPLFPLGYGL 149774
Score = 27.2 bits (53), Expect(3) = 3e-022
Identities = 9/10 (90%), Positives = 10/10 (100%)
Frame = +1 / -1
Query: 277 DPLFPLGFGL 306
DPLFPLG+GL
Sbjct: 116401 DPLFPLGYGL 116372
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 438,912,212
Number of Sequences: 392609
Number of extensions: 5086605
Number of successful extensions: 41801
Number of sequences better than 1.0e-020: 13
length of database: 147,244,331
effective HSP length: 53
effective length of database: 126,436,054
effective search space used: 17321739398
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)