TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2419405.2.3
(1600 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG455193.1|BG455193 NF100G04PL1F1034 Phosphate starved l... 90 2e-032
gb|BG453428.1|BG453428 NF091G11LF1F1085 Developing leaf Med... 104 2e-029
gb|CA917715.1|CA917715 EST641862 GPOD Medicago truncatula c... 90 1e-028
gb|AL379968.1|AL379968 MtBB48E05F1 MtBB Medicago truncatula... 90 7e-027
gb|AC148226.7| Medicago truncatula clone mth2-5i20, complet... 52 4e-025
gb|AC174309.3| Medicago truncatula clone mth2-139k12, WORKI... 52 1e-024
gb|BG448066.1|BG448066 NF105C08EC1F1056 Elicited cell cultu... 79 2e-023
>gb|BG455193.1|BG455193 NF100G04PL1F1034 Phosphate starved leaf Medicago truncatula cDNA
clone NF100G04PL 5', mRNA sequence
Length = 654
Score = 90.4 bits (191), Expect(3) = 2e-032
Identities = 36/82 (43%), Positives = 51/82 (62%)
Frame = -3 / +2
Query: 1115 PVSKYHFDLSGTAFGRLAKPGLNDKLRHSGIIDIEFTRVPCEFPGLKIGFHVEEYSNPVY 936
P + HFDLSG AFG +A G N +LR+ G I + + R C + G I F V E S P +
Sbjct: 191 PSDRTHFDLSGAAFGHMAVHGENGQLRNRGEIQVLYRRTTCVYAGKNIAFQVNEGSTPFW 370
Query: 935 FAVLVEYEDGDGDVVQVDLMES 870
++LVE+EDGDGD+ + + E+
Sbjct: 371 LSLLVEFEDGDGDISSMHIQEA 436
Score = 52.8 bits (109), Expect(3) = 2e-032
Identities = 19/33 (57%), Positives = 23/33 (69%)
Frame = -3 / +2
Query: 1226 LFKDGKGCGSCYKIRCRKDPSCSGRTETVIITD 1128
L+K G+GCG CYK++C CS R TVIITD
Sbjct: 77 LYKKGEGCGECYKVKCLDHTICSKRAVTVIITD 175
Score = 36.8 bits (74), Expect(3) = 2e-032
Identities = 11/27 (40%), Positives = 21/27 (77%)
Frame = -3 / +2
Query: 782 MQPPFSIRIRNESGKTLVANNVIPANW 702
++ PFS+++ +GK+L A +VIP++W
Sbjct: 503 LRGPFSVKLSTSTGKSLTAKDVIPSHW 583
>gb|BG453428.1|BG453428 NF091G11LF1F1085 Developing leaf Medicago truncatula cDNA clone
NF091G11LF 5', mRNA sequence
Length = 638
Score = 104 bits (221), Expect(2) = 2e-029
Identities = 42/80 (52%), Positives = 59/80 (73%)
Frame = -3 / +3
Query: 1109 SKYHFDLSGTAFGRLAKPGLNDKLRHSGIIDIEFTRVPCEFPGLKIGFHVEEYSNPVYFA 930
++YHFDLSG+AFG +A G +++LR++G I IE RV C + G I FHV+ SN YFA
Sbjct: 18 AEYHFDLSGSAFGSMAVSGEDEELRNAGKIVIEHRRVQCNYGGSTIAFHVDSGSNQEYFA 197
Query: 929 VLVEYEDGDGDVVQVDLMES 870
L+EYE+GDGD+ +V+L E+
Sbjct: 198 ALIEYEEGDGDLNKVELKEA 257
Score = 47.3 bits (97), Expect(2) = 2e-029
Identities = 17/28 (60%), Positives = 22/28 (78%)
Frame = -3 / +3
Query: 749 ESGKTLVANNVIPANWRPNTFYRSFVQY 666
+SGKT+VA+NVIPA W+P YRS V +
Sbjct: 366 KSGKTIVAHNVIPAGWKPGQTYRSIVNF 449
>gb|CA917715.1|CA917715 EST641862 GPOD Medicago truncatula cDNA clone GPOD-37E9, mRNA
sequence
Length = 769
Score = 90.4 bits (191), Expect(3) = 1e-028
Identities = 36/82 (43%), Positives = 51/82 (62%)
Frame = -3 / +2
Query: 1115 PVSKYHFDLSGTAFGRLAKPGLNDKLRHSGIIDIEFTRVPCEFPGLKIGFHVEEYSNPVY 936
P + HFDLSG AFG +A G N +LR+ G I + + R C + G I F V E S P +
Sbjct: 443 PSDRTHFDLSGAAFGHMAVHGENGQLRNRGEIQVLYRRTTCVYAGKNIAFQVNEGSTPFW 622
Query: 935 FAVLVEYEDGDGDVVQVDLMES 870
++LVE+EDGDGD+ + + E+
Sbjct: 623 LSLLVEFEDGDGDISSMHIQEA 688
Score = 50.6 bits (104), Expect(3) = 1e-028
Identities = 18/33 (54%), Positives = 22/33 (66%)
Frame = -3 / +2
Query: 1226 LFKDGKGCGSCYKIRCRKDPSCSGRTETVIITD 1128
L+ G+GCG CYK++C CS R TVIITD
Sbjct: 329 LYNKGEGCGECYKVKCLDHTICSKRAVTVIITD 427
Score = 25.8 bits (50), Expect(3) = 1e-028
Identities = 6/17 (35%), Positives = 9/17 (52%)
Frame = -3 / +2
Query: 848 TGRWTPMRESWGSVWRM 798
+ W M WG++W M
Sbjct: 692 SSEWLQMNHLWGAIWNM 742
>gb|AL379968.1|AL379968 MtBB48E05F1 MtBB Medicago truncatula cDNA clone MtBB48E05 T3, mRNA
sequence
Length = 476
Score = 90.4 bits (191), Expect(2) = 7e-027
Identities = 36/82 (43%), Positives = 51/82 (62%)
Frame = -3 / +2
Query: 1115 PVSKYHFDLSGTAFGRLAKPGLNDKLRHSGIIDIEFTRVPCEFPGLKIGFHVEEYSNPVY 936
P + HFDLSG AFG +A G N +LR+ G I + + R C + G I F V E S P +
Sbjct: 119 PSDRTHFDLSGAAFGHMAVHGENGQLRNRGEIQVLYRRTTCVYAGKNIAFQVNEGSTPFW 298
Query: 935 FAVLVEYEDGDGDVVQVDLMES 870
++LVE+EDGDGD+ + + E+
Sbjct: 299 LSLLVEFEDGDGDISSMHIQEA 364
Score = 52.8 bits (109), Expect(2) = 7e-027
Identities = 19/33 (57%), Positives = 23/33 (69%)
Frame = -3 / +2
Query: 1226 LFKDGKGCGSCYKIRCRKDPSCSGRTETVIITD 1128
L+K G+GCG CYK++C CS R TVIITD
Sbjct: 5 LYKKGEGCGECYKVKCLDHTICSKRAVTVIITD 103
>gb|AC148226.7| Medicago truncatula clone mth2-5i20, complete sequence
Length = 84259
Score = 51.9 bits (107), Expect(6) = 4e-025
Identities = 21/33 (63%), Positives = 25/33 (75%)
Frame = -3 / +1
Query: 1100 HFDLSGTAFGRLAKPGLNDKLRHSGIIDIEFTR 1002
HFDLSGTAFG LA P D LR++G I+I +TR
Sbjct: 65611 HFDLSGTAFGSLANPNQADNLRNAGKINIRYTR 65709
Score = 47.8 bits (98), Expect(6) = 4e-025
Identities = 19/40 (47%), Positives = 28/40 (70%)
Frame = -3 / +1
Query: 983 GLKIGFHVEEYSNPVYFAVLVEYEDGDGDVVQVDLMESKT 864
G I F ++ +NP FA +EYE+G+GD+VQV+L +S T
Sbjct: 65848 GNSIVFTIDNGANPYCFATEIEYENGNGDLVQVELSQSNT 65967
Score = 43.2 bits (88), Expect(6) = 4e-025
Identities = 15/29 (51%), Positives = 19/29 (65%)
Frame = -3 / +3
Query: 752 NESGKTLVANNVIPANWRPNTFYRSFVQY 666
N S T++A NVIP NW+P YRS V +
Sbjct: 70653 NGSNNTIIAENVIPQNWQPGQVYRSLVNF 70739
Score = 41.4 bits (84), Expect(6) = 4e-025
Identities = 14/28 (50%), Positives = 18/28 (64%)
Frame = -3 / +1
Query: 839 WTPMRESWGSVWRMDTNHRMQPPFSIRI 756
W PM SWGS W +T +Q PFSI++
Sbjct: 65968 WHPMFRSWGSRWAFNTGSPLQTPFSIKL 66051
Score = 35.0 bits (70), Expect(6) = 4e-025
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -3 / +2
Query: 1262 PFMSMGSCGNQPLFKDGKGCGSCYKI 1185
P M S G +F G+GCG+CY++
Sbjct: 64031 PLSKMISAGGPSIFLGGRGCGACYQV 64108
Score = 28.6 bits (56), Expect(6) = 4e-025
Identities = 8/22 (36%), Positives = 16/22 (72%)
Frame = -3 / +1
Query: 1193 YKIRCRKDPSCSGRTETVIITD 1128
++++C ++ +CSG V+ITD
Sbjct: 65521 FQVKCTENSACSGSPVNVVITD 65586
>gb|AC174309.3| Medicago truncatula clone mth2-139k12, WORKING DRAFT SEQUENCE, 2
unordered pieces
Length = 111023
Score = 51.9 bits (107), Expect(6) = 1e-024
Identities = 21/33 (63%), Positives = 25/33 (75%)
Frame = -3 / +2
Query: 1100 HFDLSGTAFGRLAKPGLNDKLRHSGIIDIEFTR 1002
HFDLSGTAFG LA P D LR++G I+I +TR
Sbjct: 92384 HFDLSGTAFGSLANPNQADNLRNAGKINIRYTR 92482
Score = 47.8 bits (98), Expect(6) = 1e-024
Identities = 19/40 (47%), Positives = 28/40 (70%)
Frame = -3 / +2
Query: 983 GLKIGFHVEEYSNPVYFAVLVEYEDGDGDVVQVDLMESKT 864
G I F ++ +NP FA +EYE+G+GD+VQV+L +S T
Sbjct: 92621 GNSIVFTIDNGANPYCFATEIEYENGNGDLVQVELSQSNT 92740
Score = 43.2 bits (88), Expect(6) = 1e-024
Identities = 15/29 (51%), Positives = 19/29 (65%)
Frame = -3 / +1
Query: 752 NESGKTLVANNVIPANWRPNTFYRSFVQY 666
N S T++A NVIP NW+P YRS V +
Sbjct: 97426 NGSNNTIIAENVIPQNWQPGQVYRSLVNF 97512
Score = 41.4 bits (84), Expect(6) = 1e-024
Identities = 14/28 (50%), Positives = 18/28 (64%)
Frame = -3 / +2
Query: 839 WTPMRESWGSVWRMDTNHRMQPPFSIRI 756
W PM SWGS W +T +Q PFSI++
Sbjct: 92741 WHPMFRSWGSRWAFNTGSPLQTPFSIKL 92824
Score = 35.0 bits (70), Expect(6) = 1e-024
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -3 / +3
Query: 1262 PFMSMGSCGNQPLFKDGKGCGSCYKI 1185
P M S G +F G+GCG+CY++
Sbjct: 90804 PLSKMISAGGPSIFLGGRGCGACYQV 90881
Score = 28.6 bits (56), Expect(6) = 1e-024
Identities = 8/22 (36%), Positives = 16/22 (72%)
Frame = -3 / +2
Query: 1193 YKIRCRKDPSCSGRTETVIITD 1128
++++C ++ +CSG V+ITD
Sbjct: 92294 FQVKCTENSACSGSPVNVVITD 92359
>gb|BG448066.1|BG448066 NF105C08EC1F1056 Elicited cell culture Medicago truncatula cDNA clone
NF105C08EC 5', mRNA sequence
Length = 652
Score = 78.5 bits (165), Expect(2) = 2e-023
Identities = 31/64 (48%), Positives = 41/64 (64%)
Frame = -3 / +1
Query: 1115 PVSKYHFDLSGTAFGRLAKPGLNDKLRHSGIIDIEFTRVPCEFPGLKIGFHVEEYSNPVY 936
P HFDLSG AFG +A G N +LR+ G I + + R PC++PG KI FHV E S P +
Sbjct: 460 PTDTTHFDLSGAAFGHMAISGENGQLRNRGQIPVIYRRTPCKYPGKKIAFHVNEGSTPFW 639
Query: 935 FAVL 924
++L
Sbjct: 640 LSLL 651
Score = 52.8 bits (109), Expect(2) = 2e-023
Identities = 19/33 (57%), Positives = 24/33 (72%)
Frame = -3 / +1
Query: 1226 LFKDGKGCGSCYKIRCRKDPSCSGRTETVIITD 1128
LF G+GCG+CYK++C + CS R TVIITD
Sbjct: 346 LFMKGEGCGACYKVKCLDNSICSRRAVTVIITD 444
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 1,105,705,381
Number of Sequences: 392609
Number of extensions: 13074026
Number of successful extensions: 60984
Number of sequences better than 1.0e-020: 11
length of database: 147,244,331
effective HSP length: 56
effective length of database: 125,258,227
effective search space used: 59622916052
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)