TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2419390.2.1
(1603 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA921322.1|CA921322 EST639040 MTUS Medicago truncatula c... 244 2e-087
gb|BQ124593.1|BQ124593 EST610169 GLSD Medicago truncatula c... 324 3e-087
gb|CA858045.1|CA858045 EST635300 GLSD Medicago truncatula c... 237 5e-085
emb|CR342881.1| mte1-75H16FM1 BAC end, cultivar Jemalong A1... 83 3e-042
gb|BI271863.1|BI271863 NF013H11FL1F1095 Developing flower M... 137 4e-031
gb|CB891913.1|CB891913 EST648882 KV3 Medicago truncatula cD... 101 2e-028
gb|AC157647.19| Medicago truncatula clone mth2-66k5, WORKIN... 70 2e-028
gb|AC147496.19| Medicago truncatula clone mth2-6i7, complet... 70 4e-028
>gb|CA921322.1|CA921322 EST639040 MTUS Medicago truncatula cDNA clone MTUS-39A2, mRNA
sequence
Length = 818
Score = 244 bits (528), Expect(2) = 2e-087
Identities = 95/159 (59%), Positives = 125/159 (78%)
Frame = -1 / -1
Query: 1117 PDLPEAKNLKSWYDSEGKDTSLAPISAEVGATRAGGFKSMYSDRVFLSHITSDPAMGQEK 938
P++PEA+ L+ WYDSEGKD ++A IS+ ++ G +S+YSDRV LS+ITS+ ++G EK
Sbjct: 812 PEVPEAQKLRCWYDSEGKDAAMAAISSGSISSSNSGSRSVYSDRVPLSYITSNLSLGAEK 633
Query: 937 PVFFSLYAIISHIKPDQNMWYRACTTCNKKVTEAFGSGYWCEGCQKNDSECSLRYIMVIK 758
P FFS+ IS IK DQ MWYRAC TCNKKVTE +GYWCE CQKND+EC+ RYIMV K
Sbjct: 632 PAFFSIRGNISFIKADQAMWYRACKTCNKKVTEDMDAGYWCESCQKNDAECNFRYIMVAK 453
Query: 757 LSDPTGEAWVSVFNEHAEKIIGCSADELDRIRKEEGDDS 641
+SD +GEA+VS FNE AEKIIGC A+ELD ++ ++G+++
Sbjct: 452 VSDASGEAFVSTFNEEAEKIIGCPAEELDNLKSQDGEEN 336
Score = 126 bits (269), Expect(3) = 4e-045
Identities = 60/107 (56%), Positives = 70/107 (65%)
Frame = +3 / +1
Query: 693 PMIFSACSLNTDTQASPVGSESLITMMYLSEHSESFFWHPSHQYPDPKASVTFLLQVVQA 872
PMIFSA SL +T+ASP+ SE+ T++YL HS SFFW SHQYP +SVTFLLQV+QA
Sbjct: 388 PMIFSASSLKVETKASPLASETFATIIYLKLHSASFFWQLSHQYPASMSSVTFLLQVLQA 567
Query: 873 RYHIF*SGLMWLMMAYRLKKTGFSWPIAGSLVM*DRKTLSEYMDLKP 1013
RYHI S M L+ LK GFS P VM*+ T SE DL P
Sbjct: 568 RYHIAWSAFMKLIFPLMLKNAGFSAPKERLEVM*ESGTRSE*TDLLP 708
Score = 100 bits (214), Expect(2) = 2e-087
Identities = 39/61 (63%), Positives = 51/61 (83%)
Frame = -1 / -1
Query: 649 DDSYVLKLKEATWVPHLFRVSVTQHEYMNEKRQRITVRGEAPVDFAAESKYLLEEIAKLT 470
++ Y +KLK+ATW+PHL RVSV+Q+EY EKRQRIT R P+DFAAESK+LLE+I+K+
Sbjct: 341 ENPYDMKLKKATWIPHLLRVSVSQNEYNGEKRQRITARAVVPIDFAAESKFLLEDISKMV 162
Query: 469 A 467
A
Sbjct: 161 A 159
Score = 57.9 bits (120), Expect(3) = 4e-045
Identities = 30/53 (56%), Positives = 35/53 (66%)
Frame = +3 / +1
Query: 462 *QAVSFAISSSKYLDSAAKSTGASPLTVILCLFSFMYSCCVTLTRNR*GTQVA 620
*+A F +SS + LDSAAKS G + VILCLFS +YS TLTR R G VA
Sbjct: 154 *EATIFDMSSRRNLDSAAKSIGTTARAVILCLFSPLYSF*ETLTRKRCGIHVA 312
Score = 38.6 bits (78), Expect(3) = 4e-045
Identities = 18/27 (66%), Positives = 19/27 (70%)
Frame = +3 / +1
Query: 1041 LIGASEVSLPSESYQDLRFLASGKSGL 1121
LI A SLPSESYQ L F ASG SG+
Sbjct: 736 LIAAIAASLPSESYQHLSF*ASGTSGM 816
>gb|BQ124593.1|BQ124593 EST610169 GLSD Medicago truncatula cDNA clone pGLSD-35P17, mRNA
sequence
Length = 639
Score = 324 bits (701), Expect = 3e-087
Identities = 131/205 (63%), Positives = 161/205 (78%)
Frame = -1 / +3
Query: 1276 VTISLWNDLATTTGQELLDMVDSSPVVAIKSLKVSDFQGVSLSTIGRSTLEINPDLPEAK 1097
+ +SLW DLAT GQELLDM D SPVVAIKSLKV DFQG+SLS I RS + +NP++PEA+
Sbjct: 24 LVVSLWGDLATNIGQELLDMADKSPVVAIKSLKVGDFQGISLSAISRSLVLVNPEVPEAQ 203
Query: 1096 NLKSWYDSEGKDTSLAPISAEVGATRAGGFKSMYSDRVFLSHITSDPAMGQEKPVFFSLY 917
L+ WYDSEGKD ++A IS+ ++ G +S+YSDRV LS+ITS+ ++G EKP FFS+
Sbjct: 204 KLRCWYDSEGKDAAMAAISSGSISSSNSGSRSVYSDRVPLSYITSNLSLGAEKPAFFSIR 383
Query: 916 AIISHIKPDQNMWYRACTTCNKKVTEAFGSGYWCEGCQKNDSECSLRYIMVIKLSDPTGE 737
IS IK DQ MWYRAC TCNKKVTE +GYWCE CQKND+EC+ RYIMV K+SD +GE
Sbjct: 384 GNISFIKADQAMWYRACKTCNKKVTEDMDAGYWCESCQKNDAECNFRYIMVAKVSDASGE 563
Query: 736 AWVSVFNEHAEKIIGCSADELDRIR 662
A+VS FNE AEKIIGC A+ELD ++
Sbjct: 564 AFVSTFNEEAEKIIGCPAEELDNLK 638
Score = 126 bits (269), Expect(2) = 6e-047
Identities = 60/107 (56%), Positives = 70/107 (65%)
Frame = +3 / -3
Query: 693 PMIFSACSLNTDTQASPVGSESLITMMYLSEHSESFFWHPSHQYPDPKASVTFLLQVVQA 872
PMIFSA SL +T+ASP+ SE+ T++YL HS SFFW SHQYP +SVTFLLQV+QA
Sbjct: 607 PMIFSASSLKVETKASPLASETFATIIYLKLHSASFFWQLSHQYPASMSSVTFLLQVLQA 428
Query: 873 RYHIF*SGLMWLMMAYRLKKTGFSWPIAGSLVM*DRKTLSEYMDLKP 1013
RYHI S M L+ LK GFS P VM*+ T SE DL P
Sbjct: 427 RYHIAWSAFMKLIFPLMLKNAGFSAPKERLEVM*ESGTRSE*TDLLP 287
Score = 84.5 bits (178), Expect(2) = 6e-047
Identities = 42/72 (58%), Positives = 49/72 (68%)
Frame = +3 / -3
Query: 1041 LIGASEVSLPSESYQDLRFLASGKSGLISRVLLPIVERDTPWKSDTFRLFIATTGELSTM 1220
LI A SLPSESYQ L F ASG SGL LL I +++ P KS T R IATTG+LS M
Sbjct: 259 LIAAIAASLPSESYQHLSF*ASGTSGLTRTKLLLIADKEIP*KSPTLRDLIATTGDLSAM 80
Query: 1221 SKSSWPVVVARS 1256
S +S P++VARS
Sbjct: 79 SSNSCPMLVARS 44
Score = 76.2 bits (160), Expect(2) = 9e-023
Identities = 39/108 (36%), Positives = 58/108 (53%)
Frame = +2 / -2
Query: 677 LVGAAANDLLRMLVEHGHPSLTSGIGELDHHDVSQRALRVILLAPLAPVSRPKSFSHLLV 856
L+ A NDLL L+E + SLT+ I +H++S+ AL +I LA PV FS+ LV
Sbjct: 623 LLSRAPNDLLSFLIEGRNESLTTCIRNFCNHNISEVALCIIFLATFTPVPSIHVFSNFLV 444
Query: 857 TGRASTVPHILIRLDVAYDGIQTEENRLFLAHSRITGDVRQKNSIRIH 1000
T T+PH L+ L +E RL + G VR+ N++R++
Sbjct: 443 TSLTGTIPHCLVCLYETDIPSDAKECRLLSPQGEVRGYVREWNTVRVN 300
Score = 53.3 bits (110), Expect(2) = 9e-023
Identities = 29/75 (38%), Positives = 44/75 (58%)
Frame = +2 / -2
Query: 1058 SIFAFRVIPGLKILSLRQVRINLESTSANS*KRHALEVRYF*ALYRNNRRTVNHVQKLLA 1237
SI FR+IP ++LS R I+ TSA S*+R+ L++ N R ++HV+ LL+
Sbjct: 242 SILTFRIIPASELLSFRYFWIDQN*TSAYS*QRNTLKIPNLEGFDCNYWRFISHVK*LLS 63
Query: 1238 SRSSKIIPERNSNSF 1282
S KI P+R++ F
Sbjct: 62 YVSCKITPQRHNKLF 18
>gb|CA858045.1|CA858045 EST635300 GLSD Medicago truncatula cDNA clone pGLSD-26H18, mRNA
sequence
Length = 813
Score = 237 bits (511), Expect(2) = 5e-085
Identities = 93/155 (60%), Positives = 121/155 (78%)
Frame = -1 / +2
Query: 1105 EAKNLKSWYDSEGKDTSLAPISAEVGATRAGGFKSMYSDRVFLSHITSDPAMGQEKPVFF 926
EA+ L+ WYDSEGKD ++A IS+ ++ G +S+YSDRV LS+ITS+ ++G EKP FF
Sbjct: 2 EAQKLRCWYDSEGKDAAMAAISSGSISSSNSGSRSVYSDRVPLSYITSNLSLGAEKPAFF 181
Query: 925 SLYAIISHIKPDQNMWYRACTTCNKKVTEAFGSGYWCEGCQKNDSECSLRYIMVIKLSDP 746
S+ IS IK DQ MWYRAC TCNKKVTE +GYWCE CQKND+EC+ RYIMV K+SD
Sbjct: 182 SIRGNISFIKADQAMWYRACKTCNKKVTEDMDAGYWCESCQKNDAECNFRYIMVAKVSDA 361
Query: 745 TGEAWVSVFNEHAEKIIGCSADELDRIRKEEGDDS 641
+GEA+VS FNE AEKIIGC A+ELD ++ ++G+++
Sbjct: 362 SGEAFVSTFNEEAEKIIGCPAEELDNLKSQDGEEN 466
Score = 126 bits (269), Expect(3) = 9e-043
Identities = 60/107 (56%), Positives = 70/107 (65%)
Frame = +3 / -1
Query: 693 PMIFSACSLNTDTQASPVGSESLITMMYLSEHSESFFWHPSHQYPDPKASVTFLLQVVQA 872
PMIFSA SL +T+ASP+ SE+ T++YL HS SFFW SHQYP +SVTFLLQV+QA
Sbjct: 414 PMIFSASSLKVETKASPLASETFATIIYLKLHSASFFWQLSHQYPASMSSVTFLLQVLQA 235
Query: 873 RYHIF*SGLMWLMMAYRLKKTGFSWPIAGSLVM*DRKTLSEYMDLKP 1013
RYHI S M L+ LK GFS P VM*+ T SE DL P
Sbjct: 234 RYHIAWSAFMKLIFPLMLKNAGFSAPKERLEVM*ESGTRSE*TDLLP 94
Score = 100 bits (214), Expect(2) = 5e-085
Identities = 39/61 (63%), Positives = 51/61 (83%)
Frame = -1 / +2
Query: 649 DDSYVLKLKEATWVPHLFRVSVTQHEYMNEKRQRITVRGEAPVDFAAESKYLLEEIAKLT 470
++ Y +KLK+ATW+PHL RVSV+Q+EY EKRQRIT R P+DFAAESK+LLE+I+K+
Sbjct: 461 ENPYDMKLKKATWIPHLLRVSVSQNEYNGEKRQRITARAVVPIDFAAESKFLLEDISKMV 640
Query: 469 A 467
A
Sbjct: 641 A 643
Score = 57.9 bits (120), Expect(3) = 9e-043
Identities = 30/53 (56%), Positives = 35/53 (66%)
Frame = +3 / -1
Query: 462 *QAVSFAISSSKYLDSAAKSTGASPLTVILCLFSFMYSCCVTLTRNR*GTQVA 620
*+A F +SS + LDSAAKS G + VILCLFS +YS TLTR R G VA
Sbjct: 648 *EATIFDMSSRRNLDSAAKSIGTTARAVILCLFSPLYSF*ETLTRKRCGIHVA 490
Score = 30.9 bits (61), Expect(3) = 9e-043
Identities = 15/22 (68%), Positives = 15/22 (68%)
Frame = +3 / -1
Query: 1041 LIGASEVSLPSESYQDLRFLAS 1106
LI A SLPSESYQ L F AS
Sbjct: 66 LIAAIAASLPSESYQHLSF*AS 1
>emb|CR342881.1| mte1-75H16FM1 BAC end, cultivar Jemalong A17 of Medicago truncatula,
genomic survey sequence
Length = 703
Score = 83.1 bits (175), Expect(4) = 3e-042
Identities = 34/46 (73%), Positives = 38/46 (82%)
Frame = -1 / +1
Query: 1288 SGKTVTISLWNDLATTTGQELLDMVDSSPVVAIKSLKVSDFQGVSL 1151
S KTV +SLW DLAT GQELLDM D SPVVAIKSLKV DFQG+++
Sbjct: 400 SKKTVVVSLWGDLATNIGQELLDMADKSPVVAIKSLKVGDFQGIAM 537
Score = 60.2 bits (125), Expect(4) = 3e-042
Identities = 22/35 (62%), Positives = 32/35 (91%)
Frame = -1 / +1
Query: 1390 DIVGVVQSVSPTLSVRRKIDNETIPKRDIVVADDS 1286
D+VGVV++VS T+S+RRK +NET+PKRDI +AD++
Sbjct: 211 DLVGVVKNVSSTMSIRRKSNNETVPKRDITIADET 315
Score = 44.1 bits (90), Expect(4) = 3e-042
Identities = 15/28 (53%), Positives = 22/28 (78%)
Frame = -1 / +2
Query: 1162 GVSLSTIGRSTLEINPDLPEAKNLKSWY 1079
G+SLS I RS + +NP++PEA+ L+ WY
Sbjct: 617 GISLSAISRSLVLVNPEVPEAQKLRCWY 700
Score = 43.2 bits (88), Expect(4) = 3e-042
Identities = 17/39 (43%), Positives = 24/39 (61%)
Frame = -1 / +2
Query: 1507 LNENAIVEEAEGETFLPPVQYNLVKIDQLGPYVGGRELV 1391
+ ++ + E A F+P ++N V IDQLGPYV ELV
Sbjct: 20 MGDSEVEEVANEAAFVPETKFNFVPIDQLGPYVNKSELV 136
>gb|BI271863.1|BI271863 NF013H11FL1F1095 Developing flower Medicago truncatula cDNA clone
NF013H11FL 5', mRNA sequence
Length = 547
Score = 137 bits (294), Expect = 4e-031
Identities = 56/97 (57%), Positives = 71/97 (73%)
Frame = -1 / +3
Query: 1492 IVEEAEGETFLPPVQYNLVKIDQLGPYVGGRELVDIVGVVQSVSPTLSVRRKIDNETIPK 1313
+ E A F+P ++N V IDQLGPYV ELVD+VGVV++VS T+S+R K +NET+PK
Sbjct: 243 VEEVANEAAFVPETKFNFVPIDQLGPYVNKSELVDLVGVVKNVSSTMSIRXKSNNETVPK 422
Query: 1312 RDIVVADDSGKTVTISLWNDLATTTGQELLDMVDSSP 1202
RDI +AD++ KTV +SLW DLAT Q LLDM D SP
Sbjct: 423 RDITIADETKKTVVVSLWGDLATNIXQXLLDMADKSP 533
>gb|CB891913.1|CB891913 EST648882 KV3 Medicago truncatula cDNA clone KV3-52F13, mRNA
sequence
Length = 370
Score = 101 bits (215), Expect(2) = 2e-028
Identities = 39/61 (63%), Positives = 51/61 (83%)
Frame = -1 / -1
Query: 649 DDSYVLKLKEATWVPHLFRVSVTQHEYMNEKRQRITVRGEAPVDFAAESKYLLEEIAKLT 470
++ Y +KLK+ATW+PHL RVSV+Q+EY EKRQRIT R P+DFAAESK+LLE+I+K+
Sbjct: 289 ENPYDMKLKKATWIPHLLRVSVSQYEYNGEKRQRITARAVVPIDFAAESKFLLEDISKMV 110
Query: 469 A 467
A
Sbjct: 109 A 107
Score = 47.3 bits (97), Expect(2) = 2e-028
Identities = 16/29 (55%), Positives = 24/29 (82%)
Frame = -1 / -1
Query: 727 SVFNEHAEKIIGCSADELDRIRKEEGDDS 641
S FNE AEKIIGC A+ELD ++ ++G+++
Sbjct: 370 STFNEEAEKIIGCPAEELDNLKSQDGEEN 284
>gb|AC157647.19| Medicago truncatula clone mth2-66k5, WORKING DRAFT SEQUENCE, 8 ordered
pieces
Length = 122626
Score = 69.8 bits (146), Expect(4) = 2e-028
Identities = 26/58 (44%), Positives = 44/58 (75%)
Frame = -1 / +3
Query: 1204 PVVAIKSLKVSDFQGVSLSTIGRSTLEINPDLPEAKNLKSWYDSEGKDTSLAPISAEV 1031
PV+A+K+ KV +F+G S++ I S L +NPD PEA++L+ W+D +GKD++ + IS ++
Sbjct: 73026 PVLAVKAGKVIEFRGKSINAIPISRLFVNPDFPEAQSLRLWFDQDGKDSASSSISKDI 73199
Score = 64.8 bits (135), Expect(4) = 2e-028
Identities = 28/77 (36%), Positives = 44/77 (57%)
Frame = -1 / +3
Query: 874 RACTTCNKKVTEAFGSGYWCEGCQKNDSECSLRYIMVIKLSDPTGEAWVSVFNEHAEKII 695
R+ C+KKVT+ + + C+ C + C RYI+ ++ D TG V+ FNE E I+
Sbjct: 73389 RSGERCSKKVTKTVNTRWKCDTCNQEFDVCEYRYILQAQIVDHTGLTCVTAFNEAGEDIM 73568
Query: 694 GCSADELDRIRKEEGDD 644
G SA +L ++ E+ DD
Sbjct: 73569 GYSAKDLYVLKYEQEDD 73619
Score = 42.8 bits (87), Expect(4) = 2e-028
Identities = 16/36 (44%), Positives = 26/36 (72%)
Frame = -1 / +3
Query: 1315 KRDIVVADDSGKTVTISLWNDLATTTGQELLDMVDS 1208
KR + + D+SG++V ++LW +L GQEL D+VD+
Sbjct: 72909 KRILNLKDNSGRSVELTLWGELCNREGQELKDIVDA 73016
Score = 35.4 bits (71), Expect(4) = 2e-028
Identities = 17/62 (27%), Positives = 36/62 (58%)
Frame = -1 / +3
Query: 661 KEEGDDSYVLKLKEATWVPHLFRVSVTQHEYMNEKRQRITVRGEAPVDFAAESKYLLEEI 482
++E D+ + +K + +FR+ + + E++ +I V V+++AESKY+L+ I
Sbjct: 73605 EQEDDERFRDIIKSILFNQFVFRLKIQKELCGEEQKVKIIVVKADKVNYSAESKYMLDLI 73784
Query: 481 AK 476
+K
Sbjct: 73785 SK 73790
>gb|AC147496.19| Medicago truncatula clone mth2-6i7, complete sequence
Length = 148736
Score = 69.8 bits (146), Expect(4) = 4e-028
Identities = 26/58 (44%), Positives = 44/58 (75%)
Frame = -1 / -3
Query: 1204 PVVAIKSLKVSDFQGVSLSTIGRSTLEINPDLPEAKNLKSWYDSEGKDTSLAPISAEV 1031
PV+A+K+ KV +F+G S++ I S L +NPD PEA++L+ W+D +GKD++ + IS ++
Sbjct: 147111 PVLAVKAGKVIEFRGKSINAIPISRLFVNPDFPEAQSLRLWFDQDGKDSASSSISKDI 146938
Score = 64.8 bits (135), Expect(4) = 4e-028
Identities = 28/77 (36%), Positives = 44/77 (57%)
Frame = -1 / -3
Query: 874 RACTTCNKKVTEAFGSGYWCEGCQKNDSECSLRYIMVIKLSDPTGEAWVSVFNEHAEKII 695
R+ C+KKVT+ + + C+ C + C RYI+ ++ D TG V+ FNE E I+
Sbjct: 146748 RSGERCSKKVTKTVNTRWKCDTCNQEFDVCEYRYILQAQIVDHTGLTCVTAFNEAGEDIM 146569
Query: 694 GCSADELDRIRKEEGDD 644
G SA +L ++ E+ DD
Sbjct: 146568 GYSAKDLYVLKYEQEDD 146518
Score = 42.8 bits (87), Expect(4) = 4e-028
Identities = 16/36 (44%), Positives = 26/36 (72%)
Frame = -1 / -3
Query: 1315 KRDIVVADDSGKTVTISLWNDLATTTGQELLDMVDS 1208
KR + + D+SG++V ++LW +L GQEL D+VD+
Sbjct: 147228 KRILNLKDNSGRSVELTLWGELCNREGQELKDIVDA 147121
Score = 35.4 bits (71), Expect(4) = 4e-028
Identities = 17/62 (27%), Positives = 36/62 (58%)
Frame = -1 / -3
Query: 661 KEEGDDSYVLKLKEATWVPHLFRVSVTQHEYMNEKRQRITVRGEAPVDFAAESKYLLEEI 482
++E D+ + +K + +FR+ + + E++ +I V V+++AESKY+L+ I
Sbjct: 146532 EQEDDERFRDIIKSILFNQFVFRLKIQKELCGEEQKVKIIVVKADKVNYSAESKYMLDLI 146353
Query: 481 AK 476
+K
Sbjct: 146352 SK 146347
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 1,533,465,390
Number of Sequences: 392609
Number of extensions: 22021521
Number of successful extensions: 77883
Number of sequences better than 1.0e-020: 10
length of database: 147,244,331
effective HSP length: 56
effective length of database: 125,258,227
effective search space used: 59748174279
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)