TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2418946.2.2
(710 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AW688473.2|AW688473 NF008A08ST1F1000 Developing stem Med... 268 8e-071
gb|BQ123088.1|BQ123088 EST608664 GLSD Medicago truncatula c... 266 3e-070
gb|AL381589.1|AL381589 MtBC01H02F3 MtBC Medicago truncatula... 237 1e-065
gb|AC152184.1| Medicago truncatula chromosome 7 BAC clone m... 133 8e-060
gb|AC122723.6| Medicago truncatula clone mth2-5i15, complet... 133 9e-060
gb|AL381590.1|AL381590 MtBC01H02R2 MtBC Medicago truncatula... 220 2e-056
gb|AC137603.16| Medicago truncatula clone mth2-14b10, compl... 129 1e-054
gb|BI310709.1|BI310709 EST5312459 GESD Medicago truncatula ... 189 2e-050
gb|CB895104.1|CB895104 EST647896 HOGA Medicago truncatula c... 146 8e-044
gb|AC158209.13| Medicago truncatula clone mth2-154h5, compl... 116 4e-041
gb|AC150782.12| Medicago truncatula clone mth2-116a21, WORK... 149 3e-035
gb|AC158736.12| Medicago truncatula clone mth2-165n2, WORKI... 149 3e-035
gb|AC157648.18| Medicago truncatula clone mth2-68d9, comple... 90 9e-034
gb|AC152887.20| Medicago truncatula clone mth2-91j4, WORKIN... 90 2e-033
gb|AL389186.1|AL389186 MtBC53C09R1 MtBC Medicago truncatula... 138 1e-031
emb|CR500324.1| mth2-177H7FM1 BAC end, cultivar Jemalong A1... 133 2e-030
emb|CR320381.1| mte1-46O24FM1 BAC end, cultivar Jemalong A1... 133 2e-030
gb|CG920826.1|CG920826 MBECG06TF mth2 Medicago truncatula g... 129 5e-029
emb|CR342893.1| mte1-75J4FM1 BAC end, cultivar Jemalong A17... 116 1e-028
gb|CX533175.1|CX533175 s13dNF0CG12MJ088_319513 Methyl Jasmo... 123 2e-027
gb|BQ146927.1|BQ146927 NF029F11FL1F1094 Developing flower M... 122 4e-027
gb|AC170583.6| Medicago truncatula clone mth2-19b5, complet... 90 6e-027
emb|CR302584.1| mte1-21I1RM1 BAC end, cultivar Jemalong A17... 120 2e-026
>gb|AW688473.2|AW688473 NF008A08ST1F1000 Developing stem Medicago truncatula cDNA clone
NF008A08ST 5', mRNA sequence
Length = 788
Score = 268 bits (579), Expect = 8e-071
Identities = 112/163 (68%), Positives = 134/163 (82%)
Frame = +3 / +2
Query: 54 ITKDNQSVLVYCFSWVVIFVILLVMKTVSVGRRRFSAEFQLVFRLIKGLIFITFTAIVVI 233
IT+ +QSVLVY SW++IF+IL +MK +SVGRRR SA+FQLVFRLI+G IFITF A ++I
Sbjct: 194 ITRSHQSVLVYGISWMIIFLILGLMKGISVGRRRLSADFQLVFRLIEGSIFITFLATLII 373
Query: 234 LIAIPGMTVLDIFVCILAFMPTGWGLXXXXXAIRPVIHKTGLWGSIKALARGYEILMGLL 413
LIA+ MT+ DI +CILA MPTGWG+ A +P+I KTGLWGS++ LARGYE++MGLL
Sbjct: 374 LIAVANMTIKDIIICILAVMPTGWGMLQIAQACKPLIAKTGLWGSVRXLARGYEVIMGLL 553
Query: 414 LFTPIAFLAXXXFVSEFQTRMLFNQAFSXGLQISRIXXGHKKD 542
LFTPIAFLA FVSEFQTRMLFNQAFS GLQISRI G K+D
Sbjct: 554 LFTPIAFLAWFPFVSEFQTRMLFNQAFSKGLQISRILGGQKRD 682
Score = 165 bits (355), Expect = 7e-040
Identities = 84/163 (51%), Positives = 93/163 (57%)
Frame = -2 / -3
Query: 541 SFLCPXXIREICRPLLNAWLNSILVWNSDTNXXQARKAMGVNXXXXXXXXXXXXXALIEP 362
S CP IREIC+PLL AWL SILV NS+TN A+KA+GV EP
Sbjct: 681 SLFCPPRIREICKPLLKAWLKSILV*NSETNGNHAKKAIGVKSSKPIITSYPRASXRTEP 502
Query: 361 QSPVLWITGRIAXXIXXKPHPVGMKARMQTNMSSTVMPGIAIRITTMAVNVINIRPLINR 182
QSPVL ++G A I P PVGM ARMQ MS V+ AI I +A NV+ I P IN
Sbjct: 501 QSPVLAMSGLQACAICSIPQPVGMTARMQIIMSLIVILATAINIMRVARNVMKIEPSINL 322
Query: 181 NTSWNSALNLLLPTDTVFITSKITKITTHEKQ*TSTLWLSLVI 53
NTSW SAL LPTD FI I KI HE *T TL LVI
Sbjct: 321 NTSWKSALRRRLPTDIPFIKPNIRKIIIHEIP*TKTL*CDLVI 193
>gb|BQ123088.1|BQ123088 EST608664 GLSD Medicago truncatula cDNA clone pGLSD-31G15, mRNA
sequence
Length = 673
Score = 266 bits (575), Expect = 3e-070
Identities = 113/171 (66%), Positives = 136/171 (79%)
Frame = +3 / +2
Query: 30 YHLNITKKITKDNQSVLVYCFSWVVIFVILLVMKTVSVGRRRFSAEFQLVFRLIKGLIFI 209
Y L IT+ + SVLVY SW++IF+IL +MK +SVGRRR SA+FQLVFRLI+G IFI
Sbjct: 11 YGLVYHLSITRSHFSVLVYGISWMIIFLILGLMKGISVGRRRLSADFQLVFRLIEGSIFI 190
Query: 210 TFTAIVVILIAIPGMTVLDIFVCILAFMPTGWGLXXXXXAIRPVIHKTGLWGSIKALARG 389
TF A ++ILIA+ MT+ DI +CILA MPTGWG+ A +P+I KTGLWGS++ALARG
Sbjct: 191 TFLATLIILIAVANMTIKDIIICILAVMPTGWGMLQIAQACKPLIAKTGLWGSVRALARG 370
Query: 390 YEILMGLLLFTPIAFLAXXXFVSEFQTRMLFNQAFSXGLQISRIXXGHKKD 542
YE++MGLLLFTP+AFLA FVSEFQTRMLFNQAFS GLQISRI G K+D
Sbjct: 371 YEVIMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGQKRD 523
Score = 166 bits (356), Expect = 5e-040
Identities = 86/171 (50%), Positives = 96/171 (56%)
Frame = -2 / -2
Query: 565 YSLFXVXXSFLCPXXIREICRPLLNAWLNSILVWNSDTNXXQARKAMGVNXXXXXXXXXX 386
Y+ + S CP IREIC+PLL AWL SILV NS+TN A+KA GV
Sbjct: 546 YNHSLLVRSLFCPPRIREICKPLLKAWLKSILV*NSETNGNHAKKATGVKSSKPIITSYP 367
Query: 385 XXXALIEPQSPVLWITGRIAXXIXXKPHPVGMKARMQTNMSSTVMPGIAIRITTMAVNVI 206
A EPQSPVL ++G A I P PVGM ARMQ MS V+ AI I +A NV+
Sbjct: 366 RASARTEPQSPVLAMSGLQACAICSIPQPVGMTARMQIIMSLIVILATAINIMRVARNVM 187
Query: 205 NIRPLINRNTSWNSALNLLLPTDTVFITSKITKITTHEKQ*TSTLWLSLVI 53
I P IN NTSW SAL LPTD FI I KI HE *T TL LVI
Sbjct: 186 KIEPSINLNTSWKSALRRRLPTDIPFIKPNIRKIIIHEIP*TKTLKCDLVI 34
>gb|AL381589.1|AL381589 MtBC01H02F3 MtBC Medicago truncatula cDNA clone MtBC01H02 T3, mRNA
sequence
Length = 502
Score = 237 bits (512), Expect(2) = 1e-065
Identities = 98/138 (71%), Positives = 115/138 (83%)
Frame = +3 / +2
Query: 60 KDNQSVLVYCFSWVVIFVILLVMKTVSVGRRRFSAEFQLVFRLIKGLIFITFTAIVVILI 239
K ++S LVY SW+VIFVIL VMKTVSVGRR+FSA FQLVFRLIKG+IF+TF AI+VILI
Sbjct: 89 KGSKSFLVYGISWLVIFVILFVMKTVSVGRRKFSANFQLVFRLIKGMIFVTFIAILVILI 268
Query: 240 AIPGMTVLDIFVCILAFMPTGWGLXXXXXAIRPVIHKTGLWGSIKALARGYEILMGLLLF 419
A+P MT DI VCILAFMPTGWG+ A++P++ + G WGS+K LARGYEI+MGLLLF
Sbjct: 269 ALPHMTPQDIIVCILAFMPTGWGMLQIAQALKPIVRRAGFWGSVKTLARGYEIVMGLLLF 448
Query: 420 TPIAFLAXXXFVSEFQTR 473
TP+AFLA FVSEFQTR
Sbjct: 449 TPVAFLAWFPFVSEFQTR 502
Score = 117 bits (250), Expect(3) = 1e-030
Identities = 58/107 (54%), Positives = 69/107 (64%)
Frame = -2 / -2
Query: 367 EPQSPVLWITGRIAXXIXXKPHPVGMKARMQTNMSSTVMPGIAIRITTMAVNVINIRPLI 188
+PQ+P G A I P PVGMKARMQT MS V+ G AI++T +A+ V NI PLI
Sbjct: 396 DPQNPARRTIGFNACAICNIPQPVGMKARMQTIMSCGVICGRAIKMTRIAIKVTNIIPLI 217
Query: 187 NRNTSWNSALNLLLPTDTVFITSKITKITTHEKQ*TSTLWLSLVIFF 47
NR TS ALNL LPTDTVFIT+ ITKIT+HE T L+ + F
Sbjct: 216 NRKTS*KFALNLRLPTDTVFITNNITKITSHEMPYTKKLFEPFFVIF 76
Score = 34.1 bits (68), Expect(2) = 1e-065
Identities = 13/16 (81%), Positives = 15/16 (93%)
Frame = +3 / +2
Query: 15 RYGLVYHLNITKKITK 62
+YGLVYHLNITKK +K
Sbjct: 53 QYGLVYHLNITKKGSK 100
Score = 27.6 bits (54), Expect(3) = 1e-030
Identities = 10/12 (83%), Positives = 11/12 (91%)
Frame = -2 / -2
Query: 52 FFVIFR*YTSPY 17
FFVIF+*YT PY
Sbjct: 90 FFVIFK*YTRPY 55
Score = 27.2 bits (53), Expect(3) = 1e-030
Identities = 11/16 (68%), Positives = 12/16 (75%)
Frame = -2 / -2
Query: 463 NSDTNXXQARKAMGVN 416
NS+T QARKA GVN
Sbjct: 492 NSETKGNQARKATGVN 445
>gb|AC152184.1| Medicago truncatula chromosome 7 BAC clone mte1-61c3, complete sequence
Length = 103090
Score = 133 bits (286), Expect(3) = 8e-060
Identities = 54/80 (67%), Positives = 66/80 (82%)
Frame = +3 / +1
Query: 327 AIRPVIHKTGLWGSIKALARGYEILMGLLLFTPIAFLAXXXFVSEFQTRMLFNQAFSXGL 506
A++P++ + G WGS+K LARGYEI+MGLLLFTP+AFLA FVSEFQTRMLFNQAFS GL
Sbjct: 9949 ALKPIVRRAGFWGSVKTLARGYEIVMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGL 10128
Query: 507 QISRIXXGHKKDXXTXNKE* 566
QISRI G +K+ + +KE*
Sbjct: 10129 QISRILGGQRKERASRSKE* 10188
Score = 116 bits (249), Expect(3) = 8e-060
Identities = 48/61 (78%), Positives = 55/61 (90%)
Frame = +3 / +2
Query: 129 KTVSVGRRRFSAEFQLVFRLIKGLIFITFTAIVVILIAIPGMTVLDIFVCILAFMPTGWG 308
+TVSVGRR+FSA FQLVFRLIKG+IF+TF AI+VILIA+P MT DI VCILAFMPTGWG
Sbjct: 9176 QTVSVGRRKFSANFQLVFRLIKGMIFVTFIAILVILIALPHMTPQDIIVCILAFMPTGWG 9355
Query: 309 L 311
+
Sbjct: 9356 M 9358
Score = 83.1 bits (175), Expect(4) = 3e-032
Identities = 39/59 (66%), Positives = 44/59 (74%)
Frame = -2 / -2
Query: 307 PHPVGMKARMQTNMSSTVMPGIAIRITTMAVNVINIRPLINRNTSWNSALNLLLPTDTV 131
P PVGMKARMQT MS V+ G AI++T +A+ V NI PLINR TS ALNL LPTDTV
Sbjct: 9354 PQPVGMKARMQTIMSCGVICGRAIKMTRIAIKVTNIIPLINRKTS*KFALNLRLPTDTV 9178
Score = 81.7 bits (172), Expect(4) = 3e-032
Identities = 35/50 (70%), Positives = 37/50 (74%)
Frame = -2 / -3
Query: 565 YSLFXVXXSFLCPXXIREICRPLLNAWLNSILVWNSDTNXXQARKAMGVN 416
YSL SFLCP IREIC+PLLNAWLNSI V NS+T QARKA GVN
Sbjct: 10187 YSLLREARSFLCPPRIREICKPLLNAWLNSIRV*NSETKGNQARKATGVN 10038
Score = 37.3 bits (75), Expect(3) = 8e-060
Identities = 19/37 (51%), Positives = 23/37 (62%)
Frame = +3 / +1
Query: 15 RYGLVYHLNITKKITKDNQSVLVYCFSWVVIFVILLV 125
+YGLVYHLNITKK +K L C + +ILLV
Sbjct: 8791 QYGLVYHLNITKKGSKSFLVSLDLCCLPFYLPIILLV 8901
Score = 27.6 bits (54), Expect(4) = 3e-032
Identities = 10/12 (83%), Positives = 11/12 (91%)
Frame = -2 / -3
Query: 52 FFVIFR*YTSPY 17
FFVIF+*YT PY
Sbjct: 8828 FFVIFK*YTRPY 8793
Score = 27.2 bits (53), Expect(4) = 3e-032
Identities = 10/13 (76%), Positives = 12/13 (92%)
Frame = -2 / -1
Query: 130 FITSKITKITTHE 92
FIT+ ITKIT+HE
Sbjct: 8986 FITNNITKITSHE 8948
>gb|AC122723.6| Medicago truncatula clone mth2-5i15, complete sequence
Length = 105353
Score = 133 bits (286), Expect(3) = 9e-060
Identities = 54/80 (67%), Positives = 66/80 (82%)
Frame = +3 / +1
Query: 327 AIRPVIHKTGLWGSIKALARGYEILMGLLLFTPIAFLAXXXFVSEFQTRMLFNQAFSXGL 506
A++P++ + G WGS+K LARGYEI+MGLLLFTP+AFLA FVSEFQTRMLFNQAFS GL
Sbjct: 81346 ALKPIVRRAGFWGSVKTLARGYEIVMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGL 81525
Query: 507 QISRIXXGHKKDXXTXNKE* 566
QISRI G +K+ + +KE*
Sbjct: 81526 QISRILGGQRKERASRSKE* 81585
Score = 116 bits (249), Expect(3) = 9e-060
Identities = 48/61 (78%), Positives = 55/61 (90%)
Frame = +3 / +2
Query: 129 KTVSVGRRRFSAEFQLVFRLIKGLIFITFTAIVVILIAIPGMTVLDIFVCILAFMPTGWG 308
+TVSVGRR+FSA FQLVFRLIKG+IF+TF AI+VILIA+P MT DI VCILAFMPTGWG
Sbjct: 80573 QTVSVGRRKFSANFQLVFRLIKGMIFVTFIAILVILIALPHMTPQDIIVCILAFMPTGWG 80752
Query: 309 L 311
+
Sbjct: 80753 M 80755
Score = 83.1 bits (175), Expect(4) = 3e-032
Identities = 39/59 (66%), Positives = 44/59 (74%)
Frame = -2 / -3
Query: 307 PHPVGMKARMQTNMSSTVMPGIAIRITTMAVNVINIRPLINRNTSWNSALNLLLPTDTV 131
P PVGMKARMQT MS V+ G AI++T +A+ V NI PLINR TS ALNL LPTDTV
Sbjct: 80751 PQPVGMKARMQTIMSCGVICGRAIKMTRIAIKVTNIIPLINRKTS*KFALNLRLPTDTV 80575
Score = 81.7 bits (172), Expect(4) = 3e-032
Identities = 35/50 (70%), Positives = 37/50 (74%)
Frame = -2 / -1
Query: 565 YSLFXVXXSFLCPXXIREICRPLLNAWLNSILVWNSDTNXXQARKAMGVN 416
YSL SFLCP IREIC+PLLNAWLNSI V NS+T QARKA GVN
Sbjct: 81584 YSLLREARSFLCPPRIREICKPLLNAWLNSIRV*NSETKGNQARKATGVN 81435
Score = 37.3 bits (75), Expect(3) = 9e-060
Identities = 19/37 (51%), Positives = 23/37 (62%)
Frame = +3 / +1
Query: 15 RYGLVYHLNITKKITKDNQSVLVYCFSWVVIFVILLV 125
+YGLVYHLNITKK +K L C + +ILLV
Sbjct: 80188 QYGLVYHLNITKKGSKSFLVSLDLCCLPFYLPIILLV 80298
Score = 27.6 bits (54), Expect(4) = 3e-032
Identities = 10/12 (83%), Positives = 11/12 (91%)
Frame = -2 / -1
Query: 52 FFVIFR*YTSPY 17
FFVIF+*YT PY
Sbjct: 80225 FFVIFK*YTRPY 80190
Score = 27.2 bits (53), Expect(4) = 3e-032
Identities = 10/13 (76%), Positives = 12/13 (92%)
Frame = -2 / -2
Query: 130 FITSKITKITTHE 92
FIT+ ITKIT+HE
Sbjct: 80383 FITNNITKITSHE 80345
>gb|AL381590.1|AL381590 MtBC01H02R2 MtBC Medicago truncatula cDNA clone MtBC01H02 T7, mRNA
sequence
Length = 548
Score = 220 bits (475), Expect = 2e-056
Identities = 91/132 (68%), Positives = 108/132 (81%)
Frame = +3 / +2
Query: 171 QLVFRLIKGLIFITFTAIVVILIAIPGMTVLDIFVCILAFMPTGWGLXXXXXAIRPVIHK 350
QLVFRLIKG+IF+TF AI+VILIA+P MT DI VCILAFMPTGWG+ A++P++ +
Sbjct: 2 QLVFRLIKGMIFVTFIAILVILIALPHMTPQDIIVCILAFMPTGWGMLQIAQALKPIVRR 181
Query: 351 TGLWGSIKALARGYEILMGLLLFTPIAFLAXXXFVSEFQTRMLFNQAFSXGLQISRIXXG 530
G WGS+K LARGYEI+MGLLLFTP+AFLA FVSEFQTRMLFNQAFS GLQISRI G
Sbjct: 182 AGFWGSVKTLARGYEIVMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGG 361
Query: 531 HKKDXXTXNKE* 566
+K+ + +KE*
Sbjct: 362 QRKERASRSKE* 397
Score = 107 bits (228), Expect = 2e-022
Identities = 50/131 (38%), Positives = 66/131 (50%)
Frame = +2 / +1
Query: 173 ASVPVDQGSDIYNIYSHRGNPNSDPWHDGAGHICLHPCFHAHWMGFXXDXXSDPXCDPQD 352
A +P++QG DI N Y G+ N +D A H CLH C HA+W+G D S
Sbjct: 4 ACLPINQGDDIRNFYCDSGHFNRPSTYDTARHYCLHSCLHANWLGNVTDCASIKAYSTSC 183
Query: 353 WALGLDQGSCPGLRDPDGTSPVHAHRLPRLXXVRVGIPDQDAVQPGVQQXSADLPYXXXT 532
W LG+ + SC L D G + VH+ L L I + +AVQP VQQ A+ Y T
Sbjct: 184 WILGICENSCSWL*DSHGFASVHSCCLSGLVSFCFRISNPNAVQPSVQQRFANFSYSWRT 363
Query: 533 QERXXXXEQRI 565
+E +QRI
Sbjct: 364 EEGARFSQQRI 396
Score = 81.7 bits (172), Expect(2) = 4e-030
Identities = 35/50 (70%), Positives = 37/50 (74%)
Frame = -2 / -3
Query: 565 YSLFXVXXSFLCPXXIREICRPLLNAWLNSILVWNSDTNXXQARKAMGVN 416
YSL SFLCP IREIC+PLLNAWLNSI V NS+T QARKA GVN
Sbjct: 396 YSLLREARSFLCPPRIREICKPLLNAWLNSIRV*NSETKGNQARKATGVN 247
Score = 71.2 bits (149), Expect(2) = 4e-030
Identities = 35/65 (53%), Positives = 42/65 (64%)
Frame = -2 / -3
Query: 367 EPQSPVLWITGRIAXXIXXKPHPVGMKARMQTNMSSTVMPGIAIRITTMAVNVINIRPLI 188
+PQ+P G A I P PVGMKARMQT MS V+ G AI++T +A+ V NI PLI
Sbjct: 198 DPQNPARRTIGFNACAICNIPQPVGMKARMQTIMSCGVICGRAIKMTRIAIKVTNIIPLI 19
Query: 187 NRNTS 173
NR TS
Sbjct: 18 NRKTS 4
>gb|AC137603.16| Medicago truncatula clone mth2-14b10, complete sequence
Length = 109793
Score = 129 bits (276), Expect(3) = 1e-054
Identities = 53/80 (66%), Positives = 64/80 (80%)
Frame = +3 / -3
Query: 327 AIRPVIHKTGLWGSIKALARGYEILMGLLLFTPIAFLAXXXFVSEFQTRMLFNQAFSXGL 506
A++P++ + G W S+K LARGYE++MGLLLFTP+AFLA FVSEFQTRMLFNQAFS GL
Sbjct: 60189 ALKPLVRRGGFWESVKTLARGYEVIMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGL 60010
Query: 507 QISRIXXGHKKDXXTXNKE* 566
QISRI G +K + NKE*
Sbjct: 60009 QISRILGGQRKGRSSRNKE* 59950
Score = 112 bits (240), Expect(3) = 1e-054
Identities = 44/69 (63%), Positives = 58/69 (84%)
Frame = +3 / -2
Query: 105 IFVILLVMKTVSVGRRRFSAEFQLVFRLIKGLIFITFTAIVVILIAIPGMTVLDIFVCIL 284
+F + +TVSVGRR+FSA+FQLVFRL+KGL+F+TF +I+V +IA+ MT+ DI VCIL
Sbjct: 60664 LFSFFVFFQTVSVGRRKFSADFQLVFRLMKGLVFVTFVSILVTMIALAHMTLQDIVVCIL 60485
Query: 285 AFMPTGWGL 311
AFMPTGWG+
Sbjct: 60484 AFMPTGWGM 60458
Score = 78.5 bits (165), Expect(2) = 4e-024
Identities = 36/59 (61%), Positives = 39/59 (66%)
Frame = -2 / +3
Query: 307 PHPVGMKARMQTNMSSTVMPGIAIRITTMAVNVINIRPLINRNTSWNSALNLLLPTDTV 131
P PVGMKARMQT MS VM AI +T + V N P I+R TS SALN LLPTDTV
Sbjct: 60462 PQPVGMKARMQTTMSCNVM*ARAIMVTRIETKVTNTNPFISRKTS*KSALNFLLPTDTV 60638
Score = 63.4 bits (132), Expect(2) = 4e-024
Identities = 31/50 (62%), Positives = 32/50 (64%)
Frame = -2 / +2
Query: 565 YSLFXVXXSFLCPXXIREICRPLLNAWLNSILVWNSDTNXXQARKAMGVN 416
YSL FL P IREIC+P LNA L SI V NSDT QARKA GVN
Sbjct: 59951 YSLLREERPFLWPPRIREICKPRLNA*LKSIRV*NSDTKGNQARKATGVN 60100
Score = 29.0 bits (57), Expect(3) = 1e-054
Identities = 10/12 (83%), Positives = 11/12 (91%)
Frame = +3 / -1
Query: 15 RYGLVYHLNITK 50
+YGLVYHLN TK
Sbjct: 61271 QYGLVYHLNFTK 61236
>gb|BI310709.1|BI310709 EST5312459 GESD Medicago truncatula cDNA clone pGESD8H8 5' end,
mRNA sequence
Length = 591
Score = 189 bits (407), Expect(2) = 2e-050
Identities = 75/129 (58%), Positives = 100/129 (77%)
Frame = +3 / +3
Query: 30 YHLNITKKITKDNQSVLVYCFSWVVIFVILLVMKTVSVGRRRFSAEFQLVFRLIKGLIFI 209
Y L TK +SVLVY SW+VIF+IL+++KTVSVGRR+FSA+FQLVFRL+KGL+F+
Sbjct: 165 YGLVYHLNFTKSTKSVLVYGISWLVIFLILVILKTVSVGRRKFSADFQLVFRLMKGLVFV 344
Query: 210 TFTAIVVILIAIPGMTVLDIFVCILAFMPTGWGLXXXXXAIRPVIHKTGLWGSIKALARG 389
TF +I+V +IA+ MT+ DI VCILAFMPTGWG+ A++P++ + G W S+K LARG
Sbjct: 345 TFVSILVTMIALAHMTLQDIVVCILAFMPTGWGMLQIAQALKPLVRRGGFWESVKTLARG 524
Query: 390 YEILMGLLL 416
YE++MG L
Sbjct: 525 YEVIMGFAL 551
Score = 104 bits (222), Expect(2) = 1e-021
Identities = 53/106 (50%), Positives = 62/106 (58%)
Frame = -2 / -3
Query: 367 EPQSPVLWITGRIAXXIXXKPHPVGMKARMQTNMSSTVMPGIAIRITTMAVNVINIRPLI 188
+ Q+P G A I P PVGMKARMQT MS VM AI +T + V N P I
Sbjct: 502 DSQNPPRRTRGFNACAICSIPQPVGMKARMQTTMSCNVM*ARAIMVTRIETKVTNTNPFI 323
Query: 187 NRNTSWNSALNLLLPTDTVFITSKITKITTHEKQ*TSTLWLSLVIF 50
+R TS SALN LLPTDTVF + I KIT H+ *T TL++ V F
Sbjct: 322 SRKTS*KSALNFLLPTDTVFKITNIRKITNHDMP*TRTLFVDFVKF 185
Score = 31.3 bits (62), Expect(2) = 2e-050
Identities = 13/17 (76%), Positives = 14/17 (82%)
Frame = +3 / +1
Query: 405 GLLLFTPIAFLAXXXFV 455
GLLLFTP+AFLA FV
Sbjct: 541 GLLLFTPVAFLAWFPFV 591
Score = 21.7 bits (41), Expect(2) = 1e-021
Identities = 9/13 (69%), Positives = 9/13 (69%)
Frame = -2 / -2
Query: 454 TNXXQARKAMGVN 416
T QARKA GVN
Sbjct: 590 TKGNQARKATGVN 552
>gb|CB895104.1|CB895104 EST647896 HOGA Medicago truncatula cDNA clone HOGA-33J7, mRNA
sequence
Length = 440
Score = 146 bits (313), Expect(2) = 8e-044
Identities = 58/88 (65%), Positives = 72/88 (81%)
Frame = +3 / -1
Query: 153 RFSAEFQLVFRLIKGLIFITFTAIVVILIAIPGMTVLDIFVCILAFMPTGWGLXXXXXAI 332
R SA+FQLVFRLI+G IFITF A ++ILIA+ MT+ DI +CILA MPTGWG+ A
Sbjct: 266 RLSADFQLVFRLIEGSIFITFLATLIILIAVANMTIKDIIICILAVMPTGWGMLQIAQAC 87
Query: 333 RPVIHKTGLWGSIKALARGYEILMGLLL 416
+P+I KTGLWGS++ALARGYE++MGLLL
Sbjct: 86 KPLIAKTGLWGSVRALARGYEVIMGLLL 3
Score = 52.4 bits (108), Expect(2) = 8e-044
Identities = 23/41 (56%), Positives = 30/41 (73%)
Frame = +3 / -3
Query: 30 YHLNITKKITKDNQSVLVYCFSWVVIFVILLVMKTVSVGRR 152
Y L IT+ +QSVLVY SW++IF+IL +MK +SVGRR
Sbjct: 390 YGLVYHLSITRSHQSVLVYGISWMIIFLILGLMKGISVGRR 268
>gb|AC158209.13| Medicago truncatula clone mth2-154h5, complete sequence
Length = 93864
Score = 116 bits (249), Expect(2) = 4e-041
Identities = 47/72 (65%), Positives = 58/72 (80%)
Frame = +3 / -3
Query: 327 AIRPVIHKTGLWGSIKALARGYEILMGLLLFTPIAFLAXXXFVSEFQTRMLFNQAFSXGL 506
A RPV+ G+WGS+KAL+RGYE LMG+++FTP+A LA FVSEFQTR+L+NQAFS GL
Sbjct: 54319 ACRPVVKAIGMWGSVKALSRGYEYLMGVVIFTPVAILAWFPFVSEFQTRLLYNQAFSRGL 54140
Query: 507 QISRIXXGHKKD 542
QI RI G KK+
Sbjct: 54139 QIQRILAGGKKN 54104
Score = 81.7 bits (172), Expect(2) = 4e-041
Identities = 32/74 (43%), Positives = 52/74 (70%)
Frame = +3 / -3
Query: 90 FSWVVIFVILLVMKTVSVGRRRFSAEFQLVFRLIKGLIFITFTAIVVILIAIPGMTVLDI 269
F +++ +LL ++ VS+GR+ FSA+FQL+FRL+K ++FI I++++ + T DI
Sbjct: 54844 FD*LLVAYLLLKLQVVSMGRKTFSADFQLMFRLLKLILFIGAVVILILMFTLFSFTFGDI 54665
Query: 270 FVCILAFMPTGWGL 311
F +LAF+PTGW L
Sbjct: 54664 FASLLAFLPTGWAL 54623
>gb|AC150782.12| Medicago truncatula clone mth2-116a21, WORKING DRAFT SEQUENCE, 3
unordered pieces
Length = 118726
Score = 149 bits (321), Expect = 3e-035
Identities = 61/161 (37%), Positives = 95/161 (59%)
Frame = +3 / +3
Query: 54 ITKDNQSVLVYCFSWVVIFVILLVMKTVSVGRRRFSAEFQLVFRLIKGLIFITFTAIVVI 233
I+ N S+ VY SW+ + V+ + V R ++SA+ + +RL++ L+ I ++V
Sbjct: 21786 ISAGNNSIAVYLLSWIYVVVVSGIYAVVVYARNKYSAKEHIYYRLVQFLVIILAILLIVA 21965
Query: 234 LIAIPGMTVLDIFVCILAFMPTGWGLXXXXXAIRPVIHKTGLWGSIKALARGYEILMGLL 413
L+ +DIF +LAF+PTGWGL RP + T +W + A+AR Y+IL G++
Sbjct: 21966 LLEFTEFKFVDIFTSLLAFLPTGWGLLLIAQVFRPFLQSTIIWSGVVAVARLYDILFGVI 22145
Query: 414 LFTPIAFLAXXXFVSEFQTRMLFNQAFSXGLQISRIXXGHK 536
+ TP+A L+ QTR+LFN+AFS GL+IS+I G K
Sbjct: 22146 IMTPVALLSWLPGFQNMQTRILFNEAFSRGLRISQIVTGKK 22268
>gb|AC158736.12| Medicago truncatula clone mth2-165n2, WORKING DRAFT SEQUENCE
Length = 118519
Score = 149 bits (321), Expect = 3e-035
Identities = 61/161 (37%), Positives = 95/161 (59%)
Frame = +3 / -1
Query: 54 ITKDNQSVLVYCFSWVVIFVILLVMKTVSVGRRRFSAEFQLVFRLIKGLIFITFTAIVVI 233
I+ N S+ VY SW+ + V+ + V R ++SA+ + +RL++ L+ I ++V
Sbjct: 11011 ISAGNNSIAVYLLSWIYVVVVSGIYAVVVYARNKYSAKEHIYYRLVQFLVIILAILLIVA 10832
Query: 234 LIAIPGMTVLDIFVCILAFMPTGWGLXXXXXAIRPVIHKTGLWGSIKALARGYEILMGLL 413
L+ +DIF +LAF+PTGWGL RP + T +W + A+AR Y+IL G++
Sbjct: 10831 LLEFTEFKFVDIFTSLLAFLPTGWGLLLIAQVFRPFLQSTIIWSGVVAVARLYDILFGVI 10652
Query: 414 LFTPIAFLAXXXFVSEFQTRMLFNQAFSXGLQISRIXXGHK 536
+ TP+A L+ QTR+LFN+AFS GL+IS+I G K
Sbjct: 10651 IMTPVALLSWLPGFQNMQTRILFNEAFSRGLRISQIVTGKK 10529
>gb|AC157648.18| Medicago truncatula clone mth2-68d9, complete sequence
Length = 84361
Score = 90.0 bits (190), Expect(3) = 9e-034
Identities = 38/65 (58%), Positives = 46/65 (70%)
Frame = +3 / +2
Query: 357 LWGSIKALARGYEILMGLLLFTPIAFLAXXXFVSEFQTRMLFNQAFSXGLQISRIXXGHK 536
LW S++ L+R YE +MGL++F P A L+ FVSEFQTR+LFNQAFS GLQIS I G K
Sbjct: 77870 LWASVRELSRAYEYVMGLIIFMPTAVLSWFPFVSEFQTRLLFNQAFSRGLQISMILAGKK 78049
Query: 537 KDXXT 551
T
Sbjct: 78050 DTYKT 78064
Score = 82.6 bits (174), Expect(3) = 9e-034
Identities = 28/59 (47%), Positives = 45/59 (76%)
Frame = +3 / +1
Query: 135 VSVGRRRFSAEFQLVFRLIKGLIFITFTAIVVILIAIPGMTVLDIFVCILAFMPTGWGL 311
VS+GRRRF +FQL+FR++K L+F+ F +++ +L + +TV D+F +LAFMP+GW +
Sbjct: 77482 VSMGRRRFGTDFQLMFRILKALLFLGFLSVMAVLFVVCALTVSDLFASVLAFMPSGWAI 77658
Score = 27.2 bits (53), Expect(3) = 9e-034
Identities = 8/13 (61%), Positives = 12/13 (92%)
Frame = +3 / +2
Query: 15 RYGLVYHLNITKK 53
+YG+VYHLNI ++
Sbjct: 77081 QYGIVYHLNIARR 77119
>gb|AC152887.20| Medicago truncatula clone mth2-91j4, WORKING DRAFT SEQUENCE, 2 ordered
pieces
Length = 116277
Score = 90.0 bits (190), Expect(3) = 2e-033
Identities = 38/65 (58%), Positives = 46/65 (70%)
Frame = +3 / -1
Query: 357 LWGSIKALARGYEILMGLLLFTPIAFLAXXXFVSEFQTRMLFNQAFSXGLQISRIXXGHK 536
LW S++ L+R YE +MGL++F P A L+ FVSEFQTR+LFNQAFS GLQIS I G K
Sbjct: 95964 LWASVRELSRAYEYVMGLIIFMPTAVLSWFPFVSEFQTRLLFNQAFSRGLQISMILAGKK 95785
Query: 537 KDXXT 551
T
Sbjct: 95784 DTYKT 95770
Score = 82.6 bits (174), Expect(3) = 2e-033
Identities = 28/59 (47%), Positives = 45/59 (76%)
Frame = +3 / -3
Query: 135 VSVGRRRFSAEFQLVFRLIKGLIFITFTAIVVILIAIPGMTVLDIFVCILAFMPTGWGL 311
VS+GRRRF +FQL+FR++K L+F+ F +++ +L + +TV D+F +LAFMP+GW +
Sbjct: 96352 VSMGRRRFGTDFQLMFRILKALLFLGFLSVMAVLFVVCALTVSDLFASVLAFMPSGWAI 96176
Score = 27.2 bits (53), Expect(3) = 2e-033
Identities = 8/13 (61%), Positives = 12/13 (92%)
Frame = +3 / -1
Query: 15 RYGLVYHLNITKK 53
+YG+VYHLNI ++
Sbjct: 96753 QYGIVYHLNIARR 96715
>gb|AL389186.1|AL389186 MtBC53C09R1 MtBC Medicago truncatula cDNA clone MtBC53C09 T7, mRNA
sequence
Length = 544
Score = 138 bits (295), Expect = 1e-031
Identities = 58/120 (48%), Positives = 82/120 (68%)
Frame = +3 / +1
Query: 174 LVFRLIKGLIFITFTAIVVILIAIPGMTVLDIFVCILAFMPTGWGLXXXXXAIRPVIHKT 353
LV R ++GL + A +V+ I + ++V D+F ILAF+PTGWG+ A +PV+ +
Sbjct: 1 LVLRFVQGLSLLLALAGLVVAIILTDLSVPDVFASILAFIPTGWGILSIAAAWKPVMKRL 180
Query: 354 GLWGSIKALARGYEILMGLLLFTPIAFLAXXXFVSEFQTRMLFNQAFSXGLQISRIXXGH 533
GLW I++LAR Y+ MG+L+F PIAF + FVS FQTR++FNQAFS GL+IS I G+
Sbjct: 181 GLWKFIRSLARLYDAGMGMLIFVPIAFFSWFPFVSTFQTRLMFNQAFSRGLEISLILAGN 360
>emb|CR500324.1| mth2-177H7FM1 BAC end, cultivar Jemalong A17 of Medicago
truncatula, genomic survey sequence
Length = 638
Score = 133 bits (286), Expect = 2e-030
Identities = 55/72 (76%), Positives = 63/72 (87%)
Frame = +3 / -1
Query: 327 AIRPVIHKTGLWGSIKALARGYEILMGLLLFTPIAFLAXXXFVSEFQTRMLFNQAFSXGL 506
A +P+I KTGLWGS++ALARGYE++MGLLLFTP+AFLA FVSEFQTRMLFNQAFS GL
Sbjct: 602 ACKPLIAKTGLWGSVRALARGYEVIMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGL 423
Query: 507 QISRIXXGHKKD 542
QISRI G K+D
Sbjct: 422 QISRILGGQKRD 387
>emb|CR320381.1| mte1-46O24FM1 BAC end, cultivar Jemalong A17 of Medicago
truncatula, genomic survey sequence
Length = 888
Score = 133 bits (286), Expect = 2e-030
Identities = 55/72 (76%), Positives = 63/72 (87%)
Frame = +3 / -1
Query: 327 AIRPVIHKTGLWGSIKALARGYEILMGLLLFTPIAFLAXXXFVSEFQTRMLFNQAFSXGL 506
A +P+I KTGLWGS++ALARGYE++MGLLLFTP+AFLA FVSEFQTRMLFNQAFS GL
Sbjct: 747 ACKPLIAKTGLWGSVRALARGYEVIMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGL 568
Query: 507 QISRIXXGHKKD 542
QISRI G K+D
Sbjct: 567 QISRILGGQKRD 532
>gb|CG920826.1|CG920826 MBECG06TF mth2 Medicago truncatula genomic clone 25B11, DNA
sequence
Length = 802
Score = 129 bits (276), Expect = 5e-029
Identities = 53/80 (66%), Positives = 64/80 (80%)
Frame = +3 / -1
Query: 327 AIRPVIHKTGLWGSIKALARGYEILMGLLLFTPIAFLAXXXFVSEFQTRMLFNQAFSXGL 506
A++P++ + G W S+K LARGYE++MGLLLFTP+AFLA FVSEFQTRMLFNQAFS GL
Sbjct: 682 ALKPLVRRGGFWESVKTLARGYEVIMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGL 503
Query: 507 QISRIXXGHKKDXXTXNKE* 566
QISRI G +K + NKE*
Sbjct: 502 QISRILGGQRKGRSSRNKE* 443
>emb|CR342893.1| mte1-75J4FM1 BAC end, cultivar Jemalong A17 of Medicago truncatula,
genomic survey sequence
Length = 724
Score = 116 bits (249), Expect(2) = 1e-028
Identities = 47/72 (65%), Positives = 58/72 (80%)
Frame = +3 / -1
Query: 327 AIRPVIHKTGLWGSIKALARGYEILMGLLLFTPIAFLAXXXFVSEFQTRMLFNQAFSXGL 506
A RPV+ G+WGS+KAL+RGYE LMG+++FTP+A LA FVSEFQTR+L+NQAFS GL
Sbjct: 367 ACRPVVKAIGMWGSVKALSRGYEYLMGVVIFTPVAILAWFPFVSEFQTRLLYNQAFSRGL 188
Query: 507 QISRIXXGHKKD 542
QI RI G KK+
Sbjct: 187 QIQRILAGGKKN 152
Score = 33.1 bits (66), Expect(2) = 1e-028
Identities = 11/16 (68%), Positives = 13/16 (81%)
Frame = +3 / -1
Query: 264 DIFVCILAFMPTGWGL 311
DIF +LAF+PTGW L
Sbjct: 718 DIFASLLAFLPTGWAL 671
>gb|CX533175.1|CX533175 s13dNF0CG12MJ088_319513 Methyl Jasmonate-Elicited Root Cell
Suspension Culture Medicago truncatula cDNA, mRNA
sequence
Length = 611
Score = 123 bits (264), Expect = 2e-027
Identities = 49/111 (44%), Positives = 75/111 (67%)
Frame = +3 / +2
Query: 219 AIVVILIAIPGMTVLDIFVCILAFMPTGWGLXXXXXAIRPVIHKTGLWGSIKALARGYEI 398
A V +++A +++ D+F ILAF+PTGWG+ + ++ GLW S++ AR Y+
Sbjct: 17 AAVCLVVAFTPLSIPDLFASILAFIPTGWGILSLAITWKGIMKSLGLWESVREFARMYDA 196
Query: 399 LMGLLLFTPIAFLAXXXFVSEFQTRMLFNQAFSXGLQISRIXXGHKKDXXT 551
MG+++F+PIAFL+ F+S FQ+R+LFNQAFS GL+IS I G+K + T
Sbjct: 197 GMGMIIFSPIAFLSWFPFISTFQSRLLFNQAFSRGLEISLILSGNKANVET 349
>gb|BQ146927.1|BQ146927 NF029F11FL1F1094 Developing flower Medicago truncatula cDNA clone
NF029F11FL 5', mRNA sequence
Length = 694
Score = 122 bits (262), Expect = 4e-027
Identities = 51/150 (34%), Positives = 84/150 (56%)
Frame = +3 / +3
Query: 54 ITKDNQSVLVYCFSWVVIFVILLVMKTVSVGRRRFSAEFQLVFRLIKGLIFITFTAIVVI 233
I+ N S+ VY SW+ + V+ + V R ++SA+ + +RL++ L+ I ++V
Sbjct: 195 ISAGNNSIAVYLLSWIYVVVVSGIYAVVVYARNKYSAKEHIYYRLVQFLVIILAILLIVA 374
Query: 234 LIAIPGMTVLDIFVCILAFMPTGWGLXXXXXAIRPVIHKTGLWGSIKALARGYEILMGLL 413
L+ +DIF +LAF+PTGWGL RP + T +W + A AR Y+IL G++
Sbjct: 375 LLEFTEFKFVDIFTSLLAFLPTGWGLLLIAQVFRPFLQSTIIWSGVXAXARLYDILFGVI 554
Query: 414 LFTPIAFLAXXXFVSEFQTRMLFNQAFSXG 503
+ TP A L+ ++S++ F+ S G
Sbjct: 555 IMTPXALLSXGAWLSKYANLXFFSTKHSXG 644
>gb|AC170583.6| Medicago truncatula clone mth2-19b5, complete sequence
Length = 113632
Score = 90.0 bits (190), Expect(3) = 6e-027
Identities = 38/69 (55%), Positives = 51/69 (73%)
Frame = +3 / +2
Query: 327 AIRPVIHKTGLWGSIKALARGYEILMGLLLFTPIAFLAXXXFVSEFQTRMLFNQAFSXGL 506
A +PV+ + GLW I++LAR Y+ MG+L+F PIAF + FVS FQTR++FNQAFS GL
Sbjct: 16574 AWKPVMKRLGLWKFIRSLARLYDAGMGMLIFVPIAFFSWFPFVSTFQTRLMFNQAFSRGL 16753
Query: 507 QISRIXXGH 533
+IS I G+
Sbjct: 16754 EISLILAGN 16780
Score = 60.2 bits (125), Expect(3) = 6e-027
Identities = 23/54 (42%), Positives = 36/54 (66%)
Frame = +3 / +3
Query: 150 RRFSAEFQLVFRLIKGLIFITFTAIVVILIAIPGMTVLDIFVCILAFMPTGWGL 311
++ S FQLV R ++GL + A +V+ I + ++V D+F ILAF+PTGWG+
Sbjct: 11739 QKISVNFQLVLRFVQGLSLLLALAGLVVAIILTDLSVPDVFASILAFIPTGWGI 11900
Score = 27.2 bits (53), Expect(3) = 6e-027
Identities = 11/33 (33%), Positives = 18/33 (54%)
Frame = +3 / +2
Query: 15 RYGLVYHLNITKKITKDNQSVLVYCFSWVVIFV 113
+YG+VY LNI T + + CFS+ + +
Sbjct: 10601 QYGIVYKLNIKGTDTSLTVCLCLICFSFEFLLI 10699
>emb|CR302584.1| mte1-21I1RM1 BAC end, cultivar Jemalong A17 of Medicago truncatula,
genomic survey sequence
Length = 651
Score = 120 bits (257), Expect = 2e-026
Identities = 48/141 (34%), Positives = 79/141 (56%)
Frame = +3 / -1
Query: 54 ITKDNQSVLVYCFSWVVIFVILLVMKTVSVGRRRFSAEFQLVFRLIKGLIFITFTAIVVI 233
I+ N S+ VY SW+ + V+ + V R ++SA+ + +RL++ L+ I ++V
Sbjct: 423 ISAGNNSIAVYLLSWIYVVVVSGIYAVVVYARNKYSAKEHIYYRLVQFLVIILAILLIVA 244
Query: 234 LIAIPGMTVLDIFVCILAFMPTGWGLXXXXXAIRPVIHKTGLWGSIKALARGYEILMGLL 413
L+ +DIF +LAF+PTGWGL RP + T +W + A+AR Y+IL G++
Sbjct: 243 LLEFTEFKFVDIFTSLLAFLPTGWGLLLIAQVFRPFLQSTIIWSGVVAVARLYDILFGVI 64
Query: 414 LFTPIAFLAXXXFVSEFQTRM 476
+ TP+A L+ QTR+
Sbjct: 63 IMTPVALLSWLPGFQNMQTRI 1
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 490,429,844
Number of Sequences: 392609
Number of extensions: 5823845
Number of successful extensions: 26446
Number of sequences better than 1.0e-020: 28
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 22939906990
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)