TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2405087.2.1
(737 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AC144540.6| Medicago truncatula clone mth2-19p16, comple... 108 1e-063
gb|AC144657.6| Medicago truncatula clone mth2-16a6, complet... 108 2e-063
gb|AC144404.34| Medicago truncatula clone mth2-19f21, WORKI... 108 5e-062
gb|AW773805.1|AW773805 EST332791 KV3 Medicago truncatula cD... 127 4e-055
gb|CG946814.1|CG946814 MBEHR23TF mth2 Medicago truncatula g... 108 2e-052
gb|CG945884.1|CG945884 MBEJM61TRB mth2 Medicago truncatula ... 108 6e-049
gb|BF637912.1|BF637912 NF028G04PL1F1034 Phosphate starved l... 150 2e-045
gb|BQ148181.1|BQ148181 NF062F03FL1F1031 Developing flower M... 148 6e-041
gb|BF642623.1|BF642623 NF070H08IN1F1075 Insect herbivory Me... 150 6e-040
gb|DW019434.1|DW019434 EST1228395 MTY Medicago truncatula c... 108 4e-037
gb|AL384586.1|AL384586 MtBC23B07F1 MtBC Medicago truncatula... 150 2e-035
gb|AW688128.1|AW688128 NF002F05ST1F1000 Developing stem Med... 108 7e-027
gb|CX517953.1|CX517953 s13dNF24C03VI022_421619 Virus-Infect... 111 1e-023
gb|AC157648.18| Medicago truncatula clone mth2-68d9, comple... 52 8e-023
gb|AC152887.20| Medicago truncatula clone mth2-91j4, WORKIN... 52 2e-022
>gb|AC144540.6| Medicago truncatula clone mth2-19p16, complete sequence
Length = 78738
Score = 108 bits (231), Expect(4) = 1e-063
Identities = 44/87 (50%), Positives = 59/87 (67%)
Frame = +2 / -3
Query: 131 PISGRKNIIVISVDSVNKEGLVRIIRNAIEVIHKENMSGSTGFVLSTSLTISEIHSLLLS 310
P+ R + VI+VD GL+ +I+ + E GS GF+LSTS+TISEI S L+S
Sbjct: 55246 PLRSRNRLFVIAVDCDTTSGLLEMIKVIFKAAGAERADGSVGFILSTSMTISEIQSFLIS 55067
Query: 311 GGMLPTDFDAFICNSGSNIYYPSHSRE 391
GG+ P DFDA+ICNSGS++YYPS + E
Sbjct: 55066 GGLSPNDFDAYICNSGSDLYYPSLNSE 54986
Score = 81.3 bits (171), Expect(4) = 1e-063
Identities = 33/68 (48%), Positives = 42/68 (61%)
Frame = +2 / -3
Query: 398 NNSKITFALDQNHQSHIEYRWGGEGLRKYLVKWATSVVERKGRTERQIIFEDPEHSSAYC 577
N+ F D SHIEYRWGGEGLRK LV+WA S ++KG + QI+ + S+ YC
Sbjct: 54994 NSEDRLFVGDLYFHSHIEYRWGGEGLRKTLVRWAASTTDKKGESNEQIVSPVEQLSTDYC 54815
Query: 578 LAFRVVNP 601
AF+V P
Sbjct: 54814 YAFKVRKP 54791
Score = 75.3 bits (158), Expect(4) = 6e-038
Identities = 38/79 (48%), Positives = 49/79 (62%)
Frame = -3 / +3
Query: 378 EG**MLLPLLQMKASKSVGSMPPDSSSECISDIVKEVDSTKPVEPDMFSLCMTSIAFLII 199
+G** LPLLQ+ ASKS+G PP+ ++CISDIV EVD P +P S LII
Sbjct: 54999 DG**RSLPLLQI*ASKSLGLKPPEIRNDCISDIVIEVDRMNPTDPSALSAPAALKITLII 55178
Query: 198 LTRPSLLTESTEITMIFFL 142
RP ++++ST IT FL
Sbjct: 55179 SRRPEVVSQSTAITNSRFL 55235
Score = 73.9 bits (155), Expect(4) = 1e-063
Identities = 30/42 (71%), Positives = 37/42 (88%)
Frame = +2 / -1
Query: 611 PPLKELRKLMRIQSLRCNALYNHSATRLSVVPIHASRSQALR 736
PPLKELRKLMRIQ+LRC+ +Y + TRL+V+P+ ASRSQALR
Sbjct: 54621 PPLKELRKLMRIQALRCHPIYCQNGTRLNVIPVLASRSQALR 54496
Score = 61.6 bits (128), Expect(4) = 1e-063
Identities = 23/32 (71%), Positives = 24/32 (75%)
Frame = +2 / -3
Query: 2 RENGLTNIHQFSWPEHCKNYLSRILTLGPRSP 97
R NGL NIH FSWPEHCK YLS+I T PR P
Sbjct: 55633 RLNGLKNIHLFSWPEHCKTYLSKIATCKPRHP 55538
Score = 60.2 bits (125), Expect(4) = 6e-038
Identities = 28/62 (45%), Positives = 35/62 (56%)
Frame = -3 / +3
Query: 600 GLTTLNARQYAEECSGSSKIICLSVLPFLSTTEVAHFTRYFLRPSPPQRYSICDW*F*SN 421
G TL A Q + E + IC + PFLS E A T+ FL PSPPQRYS+ +W + S
Sbjct: 54792 GFRTLKA*Q*SVESCSTGLTICSLLSPFLSVVEAAQRTKVFLNPSPPQRYSM*EWKYKSP 54971
Query: 420 AN 415
N
Sbjct: 54972 TN 54977
Score = 53.8 bits (111), Expect(4) = 6e-038
Identities = 24/32 (75%), Positives = 27/32 (84%)
Frame = -3 / +3
Query: 96 GDLGPRVSILDR*FLQCSGHENW*IFVSPFSL 1
G LG +V+ILDR* LQCSGHEN *IF +PFSL
Sbjct: 55539 GCLGLQVAILDR*VLQCSGHENK*IFFNPFSL 55634
Score = 49.2 bits (101), Expect(4) = 6e-038
Identities = 24/42 (57%), Positives = 27/42 (64%)
Frame = -3 / +2
Query: 735 LRA*ERDAWIGTTDNLVALWLYNALQRRDWILINFLNSFKGG 610
LRA ERDA G T +LV Y QRR WILIN +SF+GG
Sbjct: 54497 LRAWERDARTGITFSLVPF*QYIGWQRRAWILINLRSSFRGG 54622
>gb|AC144657.6| Medicago truncatula clone mth2-16a6, complete sequence
Length = 92738
Score = 108 bits (231), Expect(4) = 2e-063
Identities = 44/87 (50%), Positives = 59/87 (67%)
Frame = +2 / +3
Query: 131 PISGRKNIIVISVDSVNKEGLVRIIRNAIEVIHKENMSGSTGFVLSTSLTISEIHSLLLS 310
P+ R + VI+VD GL+ +I+ + E GS GF+LSTS+TISEI S L+S
Sbjct: 55596 PLRSRNRLFVIAVDCDTTSGLLEMIKVIFKAAGAERADGSVGFILSTSMTISEIQSFLIS 55775
Query: 311 GGMLPTDFDAFICNSGSNIYYPSHSRE 391
GG+ P DFDA+ICNSGS++YYPS + E
Sbjct: 55776 GGLSPNDFDAYICNSGSDLYYPSLNSE 55856
Score = 81.3 bits (171), Expect(4) = 2e-063
Identities = 33/68 (48%), Positives = 42/68 (61%)
Frame = +2 / +3
Query: 398 NNSKITFALDQNHQSHIEYRWGGEGLRKYLVKWATSVVERKGRTERQIIFEDPEHSSAYC 577
N+ F D SHIEYRWGGEGLRK LV+WA S ++KG + QI+ + S+ YC
Sbjct: 55848 NSEDRLFVGDLYFHSHIEYRWGGEGLRKTLVRWAASTTDKKGESNEQIVSPVEQLSTDYC 56027
Query: 578 LAFRVVNP 601
AF+V P
Sbjct: 56028 YAFKVRKP 56051
Score = 75.3 bits (158), Expect(4) = 9e-038
Identities = 38/79 (48%), Positives = 49/79 (62%)
Frame = -3 / -2
Query: 378 EG**MLLPLLQMKASKSVGSMPPDSSSECISDIVKEVDSTKPVEPDMFSLCMTSIAFLII 199
+G** LPLLQ+ ASKS+G PP+ ++CISDIV EVD P +P S LII
Sbjct: 55843 DG**RSLPLLQI*ASKSLGLKPPEIRNDCISDIVIEVDRMNPTDPSALSAPAALKITLII 55664
Query: 198 LTRPSLLTESTEITMIFFL 142
RP ++++ST IT FL
Sbjct: 55663 SRRPEVVSQSTAITNSRFL 55607
Score = 73.9 bits (155), Expect(4) = 2e-063
Identities = 30/42 (71%), Positives = 37/42 (88%)
Frame = +2 / +1
Query: 611 PPLKELRKLMRIQSLRCNALYNHSATRLSVVPIHASRSQALR 736
PPLKELRKLMRIQ+LRC+ +Y + TRL+V+P+ ASRSQALR
Sbjct: 56221 PPLKELRKLMRIQALRCHPIYCQNGTRLNVIPVLASRSQALR 56346
Score = 61.6 bits (128), Expect(4) = 2e-063
Identities = 23/32 (71%), Positives = 24/32 (75%)
Frame = +2 / +3
Query: 2 RENGLTNIHQFSWPEHCKNYLSRILTLGPRSP 97
R NGL NIH FSWPEHCK YLS+I T PR P
Sbjct: 55209 RLNGLKNIHLFSWPEHCKTYLSKIATCKPRHP 55304
Score = 60.2 bits (125), Expect(4) = 9e-038
Identities = 28/62 (45%), Positives = 35/62 (56%)
Frame = -3 / -2
Query: 600 GLTTLNARQYAEECSGSSKIICLSVLPFLSTTEVAHFTRYFLRPSPPQRYSICDW*F*SN 421
G TL A Q + E + IC + PFLS E A T+ FL PSPPQRYS+ +W + S
Sbjct: 56050 GFRTLKA*Q*SVESCSTGLTICSLLSPFLSVVEAAQRTKVFLNPSPPQRYSM*EWKYKSP 55871
Query: 420 AN 415
N
Sbjct: 55870 TN 55865
Score = 53.8 bits (111), Expect(4) = 9e-038
Identities = 24/32 (75%), Positives = 27/32 (84%)
Frame = -3 / -2
Query: 96 GDLGPRVSILDR*FLQCSGHENW*IFVSPFSL 1
G LG +V+ILDR* LQCSGHEN *IF +PFSL
Sbjct: 55303 GCLGLQVAILDR*VLQCSGHENK*IFFNPFSL 55208
Score = 49.2 bits (101), Expect(4) = 9e-038
Identities = 24/42 (57%), Positives = 27/42 (64%)
Frame = -3 / -1
Query: 735 LRA*ERDAWIGTTDNLVALWLYNALQRRDWILINFLNSFKGG 610
LRA ERDA G T +LV Y QRR WILIN +SF+GG
Sbjct: 56345 LRAWERDARTGITFSLVPF*QYIGWQRRAWILINLRSSFRGG 56220
>gb|AC144404.34| Medicago truncatula clone mth2-19f21, WORKING DRAFT SEQUENCE, 14
unordered pieces
Length = 268915
Score = 108 bits (231), Expect(4) = 5e-062
Identities = 44/87 (50%), Positives = 59/87 (67%)
Frame = +2 / +1
Query: 131 PISGRKNIIVISVDSVNKEGLVRIIRNAIEVIHKENMSGSTGFVLSTSLTISEIHSLLLS 310
P+ R + VI+VD GL+ +I+ + E GS GF+LSTS+TISEI S L+S
Sbjct: 130216 PLRSRNRLFVIAVDCDTTSGLLEMIKVIFKAAGAERADGSVGFILSTSMTISEIQSFLIS 130395
Query: 311 GGMLPTDFDAFICNSGSNIYYPSHSRE 391
GG+ P DFDA+ICNSGS++YYPS + E
Sbjct: 130396 GGLSPNDFDAYICNSGSDLYYPSLNSE 130476
Score = 81.3 bits (171), Expect(4) = 5e-062
Identities = 33/68 (48%), Positives = 42/68 (61%)
Frame = +2 / +1
Query: 398 NNSKITFALDQNHQSHIEYRWGGEGLRKYLVKWATSVVERKGRTERQIIFEDPEHSSAYC 577
N+ F D SHIEYRWGGEGLRK LV+WA S ++KG + QI+ + S+ YC
Sbjct: 130468 NSEDRLFVGDLYFHSHIEYRWGGEGLRKTLVRWAASTTDKKGESNEQIVSPVEQLSTDYC 130647
Query: 578 LAFRVVNP 601
AF+V P
Sbjct: 130648 YAFKVRKP 130671
Score = 75.3 bits (158), Expect(4) = 2e-036
Identities = 38/79 (48%), Positives = 49/79 (62%)
Frame = -3 / -3
Query: 378 EG**MLLPLLQMKASKSVGSMPPDSSSECISDIVKEVDSTKPVEPDMFSLCMTSIAFLII 199
+G** LPLLQ+ ASKS+G PP+ ++CISDIV EVD P +P S LII
Sbjct: 130463 DG**RSLPLLQI*ASKSLGLKPPEIRNDCISDIVIEVDRMNPTDPSALSAPAALKITLII 130284
Query: 198 LTRPSLLTESTEITMIFFL 142
RP ++++ST IT FL
Sbjct: 130283 SRRPEVVSQSTAITNSRFL 130227
Score = 73.9 bits (155), Expect(4) = 5e-062
Identities = 30/42 (71%), Positives = 37/42 (88%)
Frame = +2 / +2
Query: 611 PPLKELRKLMRIQSLRCNALYNHSATRLSVVPIHASRSQALR 736
PPLKELRKLMRIQ+LRC+ +Y + TRL+V+P+ ASRSQALR
Sbjct: 130841 PPLKELRKLMRIQALRCHPIYCQNGTRLNVIPVLASRSQALR 130966
Score = 61.6 bits (128), Expect(4) = 5e-062
Identities = 23/32 (71%), Positives = 24/32 (75%)
Frame = +2 / +1
Query: 2 RENGLTNIHQFSWPEHCKNYLSRILTLGPRSP 97
R NGL NIH FSWPEHCK YLS+I T PR P
Sbjct: 129829 RLNGLKNIHLFSWPEHCKTYLSKIATCKPRHP 129924
Score = 60.2 bits (125), Expect(4) = 2e-036
Identities = 28/62 (45%), Positives = 35/62 (56%)
Frame = -3 / -3
Query: 600 GLTTLNARQYAEECSGSSKIICLSVLPFLSTTEVAHFTRYFLRPSPPQRYSICDW*F*SN 421
G TL A Q + E + IC + PFLS E A T+ FL PSPPQRYS+ +W + S
Sbjct: 130670 GFRTLKA*Q*SVESCSTGLTICSLLSPFLSVVEAAQRTKVFLNPSPPQRYSM*EWKYKSP 130491
Query: 420 AN 415
N
Sbjct: 130490 TN 130485
Score = 53.8 bits (111), Expect(4) = 2e-036
Identities = 24/32 (75%), Positives = 27/32 (84%)
Frame = -3 / -3
Query: 96 GDLGPRVSILDR*FLQCSGHENW*IFVSPFSL 1
G LG +V+ILDR* LQCSGHEN *IF +PFSL
Sbjct: 129923 GCLGLQVAILDR*VLQCSGHENK*IFFNPFSL 129828
Score = 49.2 bits (101), Expect(4) = 2e-036
Identities = 24/42 (57%), Positives = 27/42 (64%)
Frame = -3 / -2
Query: 735 LRA*ERDAWIGTTDNLVALWLYNALQRRDWILINFLNSFKGG 610
LRA ERDA G T +LV Y QRR WILIN +SF+GG
Sbjct: 130965 LRAWERDARTGITFSLVPF*QYIGWQRRAWILINLRSSFRGG 130840
>gb|AW773805.1|AW773805 EST332791 KV3 Medicago truncatula cDNA clone pKV3-16A16, mRNA
sequence
Length = 642
Score = 127 bits (273), Expect(2) = 4e-055
Identities = 53/99 (53%), Positives = 66/99 (66%)
Frame = +2 / +1
Query: 398 NNSKITFALDQNHQSHIEYRWGGEGLRKYLVKWATSVVERKGRTERQIIFEDPEHSSAYC 577
N+ F D SHIEYRWGGEGLRK LV+WA S ++KG + QI+ + S+ YC
Sbjct: 346 NSEDRLFVGDLYFHSHIEYRWGGEGLRKTLVRWAASTTDKKGESNEQIVSPVEQLSTDYC 525
Query: 578 LAFRVVNPNHLPPLKELRKLMRIQSLRCNALYNHSATRL 694
AF+V P PPLKELRKLMRIQ+LRC+ +Y + TRL
Sbjct: 526 YAFKVRKPGMAPPLKELRKLMRIQALRCHPIYCQNGTRL 642
Score = 108 bits (231), Expect(2) = 4e-055
Identities = 44/87 (50%), Positives = 59/87 (67%)
Frame = +2 / +1
Query: 131 PISGRKNIIVISVDSVNKEGLVRIIRNAIEVIHKENMSGSTGFVLSTSLTISEIHSLLLS 310
P+ R + VI+VD GL+ +I+ + E GS GF+LSTS+TISEI S L+S
Sbjct: 94 PLRSRNRLFVIAVDCDTTSGLLEMIKVIFKAAGAERADGSVGFILSTSMTISEIQSFLIS 273
Query: 311 GGMLPTDFDAFICNSGSNIYYPSHSRE 391
GG+ P DFDA+ICNSGS++YYPS + E
Sbjct: 274 GGLSPNDFDAYICNSGSDLYYPSLNSE 354
Score = 88.1 bits (186), Expect(2) = 3e-033
Identities = 43/93 (46%), Positives = 53/93 (56%)
Frame = -3 / -2
Query: 693 NLVALWLYNALQRRDWILINFLNSFKGGR*LGLTTLNARQYAEECSGSSKIICLSVLPFL 514
+LV Y QRR WILIN +SF+GG G TL A Q + E + IC + PFL
Sbjct: 641 SLVPF*QYIGWQRRAWILINLRSSFRGGAIPGFRTLKA*Q*SVESCSTGLTICSLLSPFL 462
Query: 513 STTEVAHFTRYFLRPSPPQRYSICDW*F*SNAN 415
S E A T+ FL PSPPQRYS+ +W + S N
Sbjct: 461 SVVEAAQRTKVFLNPSPPQRYSM*EWKYKSPTN 363
Score = 75.3 bits (158), Expect(2) = 3e-033
Identities = 38/79 (48%), Positives = 49/79 (62%)
Frame = -3 / -2
Query: 378 EG**MLLPLLQMKASKSVGSMPPDSSSECISDIVKEVDSTKPVEPDMFSLCMTSIAFLII 199
+G** LPLLQ+ ASKS+G PP+ ++CISDIV EVD P +P S LII
Sbjct: 341 DG**RSLPLLQI*ASKSLGLKPPEIRNDCISDIVIEVDRMNPTDPSALSAPAALKITLII 162
Query: 198 LTRPSLLTESTEITMIFFL 142
RP ++++ST IT FL
Sbjct: 161 SRRPEVVSQSTAITNSRFL 105
>gb|CG946814.1|CG946814 MBEHR23TF mth2 Medicago truncatula genomic clone 58C22, DNA
sequence
Length = 869
Score = 108 bits (231), Expect(3) = 2e-052
Identities = 44/87 (50%), Positives = 59/87 (67%)
Frame = +2 / +3
Query: 131 PISGRKNIIVISVDSVNKEGLVRIIRNAIEVIHKENMSGSTGFVLSTSLTISEIHSLLLS 310
P+ R + VI+VD GL+ +I+ + E GS GF+LSTS+TISEI S L+S
Sbjct: 423 PLRSRNRLFVIAVDCDTTSGLLEMIKVIFKAAGAERADGSVGFILSTSMTISEIQSFLIS 602
Query: 311 GGMLPTDFDAFICNSGSNIYYPSHSRE 391
GG+ P DFDA+ICNSGS++YYPS + E
Sbjct: 603 GGLSPNDFDAYICNSGSDLYYPSLNSE 683
Score = 79.4 bits (167), Expect(3) = 2e-052
Identities = 32/65 (49%), Positives = 41/65 (63%)
Frame = +2 / +3
Query: 398 NNSKITFALDQNHQSHIEYRWGGEGLRKYLVKWATSVVERKGRTERQIIFEDPEHSSAYC 577
N+ F D SHIEYRWGGEGLRK LV+WA S ++KG + QI+ + S+ YC
Sbjct: 675 NSEDRLFVGDLYFHSHIEYRWGGEGLRKTLVRWAASTTDKKGESNEQIVSPVEQLSTDYC 854
Query: 578 LAFRV 592
AF+V
Sbjct: 855 YAFKV 869
Score = 75.3 bits (158), Expect(3) = 7e-034
Identities = 38/79 (48%), Positives = 49/79 (62%)
Frame = -3 / -2
Query: 378 EG**MLLPLLQMKASKSVGSMPPDSSSECISDIVKEVDSTKPVEPDMFSLCMTSIAFLII 199
+G** LPLLQ+ ASKS+G PP+ ++CISDIV EVD P +P S LII
Sbjct: 670 DG**RSLPLLQI*ASKSLGLKPPEIRNDCISDIVIEVDRMNPTDPSALSAPAALKITLII 491
Query: 198 LTRPSLLTESTEITMIFFL 142
RP ++++ST IT FL
Sbjct: 490 SRRPEVVSQSTAITNSRFL 434
Score = 61.6 bits (128), Expect(3) = 2e-052
Identities = 23/32 (71%), Positives = 24/32 (75%)
Frame = +2 / +3
Query: 2 RENGLTNIHQFSWPEHCKNYLSRILTLGPRSP 97
R NGL NIH FSWPEHCK YLS+I T PR P
Sbjct: 36 RLNGLKNIHLFSWPEHCKTYLSKIATCKPRHP 131
Score = 57.9 bits (120), Expect(3) = 7e-034
Identities = 27/59 (45%), Positives = 34/59 (57%)
Frame = -3 / -2
Query: 591 TLNARQYAEECSGSSKIICLSVLPFLSTTEVAHFTRYFLRPSPPQRYSICDW*F*SNAN 415
TL A Q + E + IC + PFLS E A T+ FL PSPPQRYS+ +W + S N
Sbjct: 868 TLKA*Q*SVESCSTGLTICSLLSPFLSVVEAAQRTKVFLNPSPPQRYSM*EWKYKSPTN 692
Score = 53.8 bits (111), Expect(3) = 7e-034
Identities = 24/32 (75%), Positives = 27/32 (84%)
Frame = -3 / -2
Query: 96 GDLGPRVSILDR*FLQCSGHENW*IFVSPFSL 1
G LG +V+ILDR* LQCSGHEN *IF +PFSL
Sbjct: 130 GCLGLQVAILDR*VLQCSGHENK*IFFNPFSL 35
>gb|CG945884.1|CG945884 MBEJM61TRB mth2 Medicago truncatula genomic clone 69K1, DNA
sequence
Length = 844
Score = 108 bits (231), Expect(3) = 6e-049
Identities = 44/87 (50%), Positives = 59/87 (67%)
Frame = +2 / +3
Query: 131 PISGRKNIIVISVDSVNKEGLVRIIRNAIEVIHKENMSGSTGFVLSTSLTISEIHSLLLS 310
P+ R + VI+VD GL+ +I+ + E GS GF+LSTS+TISEI S L+S
Sbjct: 423 PLRSRNRLFVIAVDCDTTSGLLEMIKVIFKAAGAERADGSVGFILSTSMTISEIQSFLIS 602
Query: 311 GGMLPTDFDAFICNSGSNIYYPSHSRE 391
GG+ P DFDA+ICNSGS++YYPS + E
Sbjct: 603 GGLSPNDFDAYICNSGSDLYYPSLNSE 683
Score = 75.3 bits (158), Expect(3) = 1e-032
Identities = 38/79 (48%), Positives = 49/79 (62%)
Frame = -3 / -1
Query: 378 EG**MLLPLLQMKASKSVGSMPPDSSSECISDIVKEVDSTKPVEPDMFSLCMTSIAFLII 199
+G** LPLLQ+ ASKS+G PP+ ++CISDIV EVD P +P S LII
Sbjct: 670 DG**RSLPLLQI*ASKSLGLKPPEIRNDCISDIVIEVDRMNPTDPSALSAPAALKITLII 491
Query: 198 LTRPSLLTESTEITMIFFL 142
RP ++++ST IT FL
Sbjct: 490 SRRPEVVSQSTAITNSRFL 434
Score = 67.5 bits (141), Expect(3) = 6e-049
Identities = 26/49 (53%), Positives = 32/49 (65%)
Frame = +2 / +3
Query: 398 NNSKITFALDQNHQSHIEYRWGGEGLRKYLVKWATSVVERKGRTERQII 544
N+ F D SHIEYRWGGEGLRK LV+WA S ++KG + QI+
Sbjct: 675 NSEDRLFVGDLYFHSHIEYRWGGEGLRKTLVRWAASTTDKKGESNEQIV 821
Score = 61.6 bits (128), Expect(3) = 6e-049
Identities = 23/32 (71%), Positives = 24/32 (75%)
Frame = +2 / +3
Query: 2 RENGLTNIHQFSWPEHCKNYLSRILTLGPRSP 97
R NGL NIH FSWPEHCK YLS+I T PR P
Sbjct: 36 RLNGLKNIHLFSWPEHCKTYLSKIATCKPRHP 131
Score = 53.8 bits (111), Expect(3) = 1e-032
Identities = 24/32 (75%), Positives = 27/32 (84%)
Frame = -3 / -1
Query: 96 GDLGPRVSILDR*FLQCSGHENW*IFVSPFSL 1
G LG +V+ILDR* LQCSGHEN *IF +PFSL
Sbjct: 130 GCLGLQVAILDR*VLQCSGHENK*IFFNPFSL 35
Score = 53.8 bits (111), Expect(3) = 1e-032
Identities = 23/50 (46%), Positives = 29/50 (58%)
Frame = -3 / -1
Query: 564 ECSGSSKIICLSVLPFLSTTEVAHFTRYFLRPSPPQRYSICDW*F*SNAN 415
E + IC + PFLS E A T+ FL PSPPQRYS+ +W + S N
Sbjct: 841 ESCSTGLTICSLLSPFLSVVEAAQRTKVFLNPSPPQRYSM*EWKYKSPTN 692
>gb|BF637912.1|BF637912 NF028G04PL1F1034 Phosphate starved leaf Medicago truncatula cDNA
clone NF028G04PL 5', mRNA sequence
Length = 651
Score = 150 bits (323), Expect(3) = 2e-045
Identities = 63/113 (55%), Positives = 79/113 (69%)
Frame = +2 / +1
Query: 398 NNSKITFALDQNHQSHIEYRWGGEGLRKYLVKWATSVVERKGRTERQIIFEDPEHSSAYC 577
N+ F D SHIEYRWGGEGLRK LV+WA S ++KG + QI+ + S+ YC
Sbjct: 115 NSEDRLFVGDLYFHSHIEYRWGGEGLRKTLVRWAASTTDKKGESNEQIVSPVEQLSTDYC 294
Query: 578 LAFRVVNPNHLPPLKELRKLMRIQSLRCNALYNHSATRLSVVPIHASRSQALR 736
AF+V P PPLKELRKLMRIQ+LRC+ +Y + TRL+V+P+ ASRSQALR
Sbjct: 295 YAFKVRKPGMAPPLKELRKLMRIQALRCHPIYCQNGTRLNVIPVLASRSQALR 453
Score = 102 bits (218), Expect(2) = 5e-026
Identities = 52/107 (48%), Positives = 62/107 (57%)
Frame = -3 / -2
Query: 735 LRA*ERDAWIGTTDNLVALWLYNALQRRDWILINFLNSFKGGR*LGLTTLNARQYAEECS 556
LRA ERDA G T +LV Y QRR WILIN +SF+GG G TL A Q + E
Sbjct: 452 LRAWERDARTGITFSLVPF*QYIGWQRRAWILINLRSSFRGGAIPGFRTLKA*Q*SVESC 273
Query: 555 GSSKIICLSVLPFLSTTEVAHFTRYFLRPSPPQRYSICDW*F*SNAN 415
+ IC + PFLS E A T+ FL PSPPQRYS+ +W + S N
Sbjct: 272 STGLTICSLLSPFLSVVEAAQRTKVFLNPSPPQRYSM*EWKYKSPTN 132
Score = 38.2 bits (77), Expect(3) = 2e-045
Identities = 15/21 (71%), Positives = 17/21 (80%)
Frame = +2 / +3
Query: 278 TISEIHSLLLSGGMLPTDFDA 340
TISEI S L+SGG+ P DFDA
Sbjct: 9 TISEIQSFLISGGLSPNDFDA 71
Score = 36.3 bits (73), Expect(2) = 5e-026
Identities = 14/22 (63%), Positives = 18/22 (81%)
Frame = -3 / -3
Query: 342 KASKSVGSMPPDSSSECISDIV 277
KASKS+G PP+ ++CISDIV
Sbjct: 73 KASKSLGLKPPEIRNDCISDIV 8
Score = 33.1 bits (66), Expect(3) = 2e-045
Identities = 11/17 (64%), Positives = 15/17 (88%)
Frame = +2 / +1
Query: 341 FICNSGSNIYYPSHSRE 391
+ICNSGS++YYPS + E
Sbjct: 73 YICNSGSDLYYPSLNSE 123
>gb|BQ148181.1|BQ148181 NF062F03FL1F1031 Developing flower Medicago truncatula cDNA clone
NF062F03FL 5', mRNA sequence
Length = 612
Score = 148 bits (318), Expect(2) = 6e-041
Identities = 60/99 (60%), Positives = 75/99 (75%)
Frame = +2 / +3
Query: 440 SHIEYRWGGEGLRKYLVKWATSVVERKGRTERQIIFEDPEHSSAYCLAFRVVNPNHLPPL 619
SHIEYRWGGEGLRK LV+WA S ++KG + QI+ + S+ YC AF+V P PPL
Sbjct: 96 SHIEYRWGGEGLRKTLVRWAASTTDKKGESNEQIVSPVEQLSTDYCYAFKVRKPGMAPPL 275
Query: 620 KELRKLMRIQSLRCNALYNHSATRLSVVPIHASRSQALR 736
KELRKLMRIQ+LRC+ +Y + TRL+V+P+ ASRSQALR
Sbjct: 276 KELRKLMRIQALRCHPIYCQNGTRLNVIPVLASRSQALR 392
Score = 101 bits (215), Expect(2) = 1e-020
Identities = 50/100 (50%), Positives = 59/100 (59%)
Frame = -3 / -3
Query: 735 LRA*ERDAWIGTTDNLVALWLYNALQRRDWILINFLNSFKGGR*LGLTTLNARQYAEECS 556
LRA ERDA G T +LV Y QRR WILIN +SF+GG G TL A Q + E
Sbjct: 391 LRAWERDARTGITFSLVPF*QYIGWQRRAWILINLRSSFRGGAIPGFRTLKA*Q*SVESC 212
Query: 555 GSSKIICLSVLPFLSTTEVAHFTRYFLRPSPPQRYSICDW 436
+ IC + PFLS E A T+ FL PSPPQRYS+ +W
Sbjct: 211 STGLTICSLLSPFLSVVEAAQRTKVFLNPSPPQRYSM*EW 92
Score = 40.5 bits (82), Expect(2) = 6e-041
Identities = 14/20 (70%), Positives = 18/20 (90%)
Frame = +2 / +2
Query: 332 FDAFICNSGSNIYYPSHSRE 391
FDA+ICNSGS++YYPS + E
Sbjct: 2 FDAYICNSGSDLYYPSLNSE 61
Score = 21.7 bits (41), Expect(2) = 1e-020
Identities = 11/16 (68%), Positives = 13/16 (81%)
Frame = -3 / -1
Query: 378 EG**MLLPLLQMKASK 331
+G** LPLLQ+ ASK
Sbjct: 48 DG**RSLPLLQI*ASK 1
>gb|BF642623.1|BF642623 NF070H08IN1F1075 Insect herbivory Medicago truncatula cDNA clone
NF070H08IN 5', mRNA sequence
Length = 586
Score = 150 bits (323), Expect(2) = 6e-040
Identities = 63/113 (55%), Positives = 79/113 (69%)
Frame = +2 / +1
Query: 398 NNSKITFALDQNHQSHIEYRWGGEGLRKYLVKWATSVVERKGRTERQIIFEDPEHSSAYC 577
N+ F D SHIEYRWGGEGLRK LV+WA S ++KG + QI+ + S+ YC
Sbjct: 46 NSEDRLFVGDLYFHSHIEYRWGGEGLRKTLVRWAASTTDKKGESNEQIVSPVEQLSTDYC 225
Query: 578 LAFRVVNPNHLPPLKELRKLMRIQSLRCNALYNHSATRLSVVPIHASRSQALR 736
AF+V P PPLKELRKLMRIQ+LRC+ +Y + TRL+V+P+ ASRSQALR
Sbjct: 226 YAFKVRKPGMAPPLKELRKLMRIQALRCHPIYCQNGTRLNVIPVLASRSQALR 384
Score = 102 bits (218), Expect = 5e-021
Identities = 52/107 (48%), Positives = 62/107 (57%)
Frame = -3 / -3
Query: 735 LRA*ERDAWIGTTDNLVALWLYNALQRRDWILINFLNSFKGGR*LGLTTLNARQYAEECS 556
LRA ERDA G T +LV Y QRR WILIN +SF+GG G TL A Q + E
Sbjct: 383 LRAWERDARTGITFSLVPF*QYIGWQRRAWILINLRSSFRGGAIPGFRTLKA*Q*SVESC 204
Query: 555 GSSKIICLSVLPFLSTTEVAHFTRYFLRPSPPQRYSICDW*F*SNAN 415
+ IC + PFLS E A T+ FL PSPPQRYS+ +W + S N
Sbjct: 203 STGLTICSLLSPFLSVVEAAQRTKVFLNPSPPQRYSM*EWKYKSPTN 63
Score = 35.0 bits (70), Expect(2) = 6e-040
Identities = 12/18 (66%), Positives = 16/18 (88%)
Frame = +2 / +1
Query: 338 AFICNSGSNIYYPSHSRE 391
A+ICNSGS++YYPS + E
Sbjct: 1 AYICNSGSDLYYPSLNSE 54
>gb|DW019434.1|DW019434 EST1228395 MTY Medicago truncatula cDNA clone MTYBI85, mRNA
sequence
Length = 683
Score = 108 bits (231), Expect(2) = 4e-037
Identities = 44/87 (50%), Positives = 59/87 (67%)
Frame = +2 / +1
Query: 131 PISGRKNIIVISVDSVNKEGLVRIIRNAIEVIHKENMSGSTGFVLSTSLTISEIHSLLLS 310
P+ R + VI+VD GL+ +I+ + E GS GF+LSTS+TISEI S L+S
Sbjct: 265 PLRSRNRLFVIAVDCDTTSGLLEMIKVIFKAAGAERADGSVGFILSTSMTISEIQSFLIS 444
Query: 311 GGMLPTDFDAFICNSGSNIYYPSHSRE 391
GG+ P DFDA+ICNSGS++YYPS + E
Sbjct: 445 GGLSPNDFDAYICNSGSDLYYPSLNSE 525
Score = 75.3 bits (158), Expect(2) = 5e-023
Identities = 38/79 (48%), Positives = 49/79 (62%)
Frame = -3 / -1
Query: 378 EG**MLLPLLQMKASKSVGSMPPDSSSECISDIVKEVDSTKPVEPDMFSLCMTSIAFLII 199
+G** LPLLQ+ ASKS+G PP+ ++CISDIV EVD P +P S LII
Sbjct: 512 DG**RSLPLLQI*ASKSLGLKPPEIRNDCISDIVIEVDRMNPTDPSALSAPAALKITLII 333
Query: 198 LTRPSLLTESTEITMIFFL 142
RP ++++ST IT FL
Sbjct: 332 SRRPEVVSQSTAITNSRFL 276
Score = 67.5 bits (141), Expect(2) = 4e-037
Identities = 26/49 (53%), Positives = 32/49 (65%)
Frame = +2 / +1
Query: 398 NNSKITFALDQNHQSHIEYRWGGEGLRKYLVKWATSVVERKGRTERQII 544
N+ F D SHIEYRWGGEGLRK LV+WA S ++KG + QI+
Sbjct: 517 NSEDRLFVGDLYFHSHIEYRWGGEGLRKTLVRWAASTTDKKGESNEQIV 663
Score = 53.8 bits (111), Expect(2) = 5e-023
Identities = 23/50 (46%), Positives = 29/50 (58%)
Frame = -3 / -1
Query: 564 ECSGSSKIICLSVLPFLSTTEVAHFTRYFLRPSPPQRYSICDW*F*SNAN 415
E + IC + PFLS E A T+ FL PSPPQRYS+ +W + S N
Sbjct: 683 ESCSTGLTICSLLSPFLSVVEAAQRTKVFLNPSPPQRYSM*EWKYKSPTN 534
>gb|AL384586.1|AL384586 MtBC23B07F1 MtBC Medicago truncatula cDNA clone MtBC23B07 T3, mRNA
sequence
Length = 477
Score = 150 bits (323), Expect = 2e-035
Identities = 63/113 (55%), Positives = 79/113 (69%)
Frame = +2 / +3
Query: 398 NNSKITFALDQNHQSHIEYRWGGEGLRKYLVKWATSVVERKGRTERQIIFEDPEHSSAYC 577
N+ F D SHIEYRWGGEGLRK LV+WA S ++KG + QI+ + S+ YC
Sbjct: 30 NSEDRLFVGDLYFHSHIEYRWGGEGLRKTLVRWAASTTDKKGESNEQIVSPVEQLSTDYC 209
Query: 578 LAFRVVNPNHLPPLKELRKLMRIQSLRCNALYNHSATRLSVVPIHASRSQALR 736
AF+V P PPLKELRKLMRIQ+LRC+ +Y + TRL+V+P+ ASRSQALR
Sbjct: 210 YAFKVRKPGMAPPLKELRKLMRIQALRCHPIYCQNGTRLNVIPVLASRSQALR 368
Score = 102 bits (218), Expect = 5e-021
Identities = 52/107 (48%), Positives = 62/107 (57%)
Frame = -3 / -3
Query: 735 LRA*ERDAWIGTTDNLVALWLYNALQRRDWILINFLNSFKGGR*LGLTTLNARQYAEECS 556
LRA ERDA G T +LV Y QRR WILIN +SF+GG G TL A Q + E
Sbjct: 367 LRAWERDARTGITFSLVPF*QYIGWQRRAWILINLRSSFRGGAIPGFRTLKA*Q*SVESC 188
Query: 555 GSSKIICLSVLPFLSTTEVAHFTRYFLRPSPPQRYSICDW*F*SNAN 415
+ IC + PFLS E A T+ FL PSPPQRYS+ +W + S N
Sbjct: 187 STGLTICSLLSPFLSVVEAAQRTKVFLNPSPPQRYSM*EWKYKSPTN 47
>gb|AW688128.1|AW688128 NF002F05ST1F1000 Developing stem Medicago truncatula cDNA clone
NF002F05ST 5', mRNA sequence
Length = 483
Score = 108 bits (231), Expect(2) = 7e-027
Identities = 44/87 (50%), Positives = 59/87 (67%)
Frame = +2 / +1
Query: 131 PISGRKNIIVISVDSVNKEGLVRIIRNAIEVIHKENMSGSTGFVLSTSLTISEIHSLLLS 310
P+ R + VI+VD GL+ +I+ + E GS GF+LSTS+TISEI S L+S
Sbjct: 160 PLRSRNRLFVIAVDCDTTSGLLEMIKVIFKAAGAERADGSVGFILSTSMTISEIQSFLIS 339
Query: 311 GGMLPTDFDAFICNSGSNIYYPSHSRE 391
GG+ P DFDA+ICNSGS++YYPS + E
Sbjct: 340 GGLSPNDFDAYICNSGSDLYYPSLNSE 420
Score = 33.1 bits (66), Expect(2) = 7e-027
Identities = 13/24 (54%), Positives = 14/24 (58%)
Frame = +2 / +1
Query: 398 NNSKITFALDQNHQSHIEYRWGGE 469
N+ F D SHIEYRWGGE
Sbjct: 412 NSEDRLFVGDLYFHSHIEYRWGGE 483
>gb|CX517953.1|CX517953 s13dNF24C03VI022_421619 Virus-Infected Leaves Medicago truncatula
cDNA, mRNA sequence
Length = 457
Score = 111 bits (238), Expect = 1e-023
Identities = 47/86 (54%), Positives = 61/86 (70%)
Frame = +2 / +3
Query: 479 KYLVKWATSVVERKGRTERQIIFEDPEHSSAYCLAFRVVNPNHLPPLKELRKLMRIQSLR 658
K LV+WA S ++KG + QI+ + S+ YC AF V P PPLKELRKLMRIQ+LR
Sbjct: 12 KTLVRWAASTTDKKGESNEQIVSPVEQLSTDYCYAFGVRKPGMAPPLKELRKLMRIQALR 191
Query: 659 CNALYNHSATRLSVVPIHASRSQALR 736
C+ +Y + TRL+V+P+ ASRSQALR
Sbjct: 192 CHPIYCQNGTRLNVIPVLASRSQALR 269
>gb|AC157648.18| Medicago truncatula clone mth2-68d9, complete sequence
Length = 84361
Score = 52.4 bits (108), Expect(4) = 8e-023
Identities = 21/42 (50%), Positives = 28/42 (66%)
Frame = +2 / +3
Query: 251 TGFVLSTSLTISEIHSLLLSGGMLPTDFDAFICNSGSNIYYP 376
+GF LST++ I + L SG + DFDA IC+SGS +YYP
Sbjct: 61191 SGFALSTAMPILQTIEFLKSGKIQVNDFDALICSSGSELYYP 61316
Score = 52.4 bits (108), Expect(4) = 8e-023
Identities = 18/32 (56%), Positives = 22/32 (68%)
Frame = +2 / +3
Query: 2 RENGLTNIHQFSWPEHCKNYLSRILTLGPRSP 97
R+NG NIH FSWPEHC+ YL+R+ R P
Sbjct: 60726 RKNGWKNIHLFSWPEHCRTYLTRVAACRMRHP 60821
Score = 45.5 bits (93), Expect(4) = 8e-023
Identities = 17/38 (44%), Positives = 29/38 (76%)
Frame = +2 / +2
Query: 623 ELRKLMRIQSLRCNALYNHSATRLSVVPIHASRSQALR 736
+LR+ +R++ LRC+ +Y +T + V+P+ ASR+QALR
Sbjct: 61958 DLRQKLRMRGLRCHPMYCRRSTYMQVIPLLASRAQALR 62071
Score = 36.3 bits (73), Expect(4) = 8e-023
Identities = 11/19 (57%), Positives = 17/19 (89%)
Frame = +2 / +3
Query: 425 DQNHQSHIEYRWGGEGLRK 481
D ++++HI+YRWG EGL+K
Sbjct: 61350 DPDYEAHIDYRWGCEGLKK 61406
>gb|AC152887.20| Medicago truncatula clone mth2-91j4, WORKING DRAFT SEQUENCE, 2 ordered
pieces
Length = 116277
Score = 52.4 bits (108), Expect(4) = 2e-022
Identities = 21/42 (50%), Positives = 28/42 (66%)
Frame = +2 / -2
Query: 251 TGFVLSTSLTISEIHSLLLSGGMLPTDFDAFICNSGSNIYYP 376
+GF LST++ I + L SG + DFDA IC+SGS +YYP
Sbjct: 112643 SGFALSTAMPILQTIEFLKSGKIQVNDFDALICSSGSELYYP 112518
Score = 52.4 bits (108), Expect(4) = 2e-022
Identities = 18/32 (56%), Positives = 22/32 (68%)
Frame = +2 / -2
Query: 2 RENGLTNIHQFSWPEHCKNYLSRILTLGPRSP 97
R+NG NIH FSWPEHC+ YL+R+ R P
Sbjct: 113108 RKNGWKNIHLFSWPEHCRTYLTRVAACRMRHP 113013
Score = 45.5 bits (93), Expect(4) = 2e-022
Identities = 17/38 (44%), Positives = 29/38 (76%)
Frame = +2 / -1
Query: 623 ELRKLMRIQSLRCNALYNHSATRLSVVPIHASRSQALR 736
+LR+ +R++ LRC+ +Y +T + V+P+ ASR+QALR
Sbjct: 111876 DLRQKLRMRGLRCHPMYCRRSTYMQVIPLLASRAQALR 111763
Score = 36.3 bits (73), Expect(4) = 2e-022
Identities = 11/19 (57%), Positives = 17/19 (89%)
Frame = +2 / -2
Query: 425 DQNHQSHIEYRWGGEGLRK 481
D ++++HI+YRWG EGL+K
Sbjct: 112484 DPDYEAHIDYRWGCEGLKK 112428
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 772,243,891
Number of Sequences: 392609
Number of extensions: 11351488
Number of successful extensions: 47367
Number of sequences better than 1.0e-020: 21
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 24074297995
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)