TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2276425.2.1
(753 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CG962262.1|CG962262 MBEEP40TRB mth2 Medicago truncatula ... 313 2e-084
gb|CA920561.1|CA920561 EST638279 MTUS Medicago truncatula c... 288 8e-077
gb|BG647806.1|BG647806 EST509425 HOGA Medicago truncatula c... 254 9e-067
gb|BE202431.1|BE202431 EST392880 KV1 Medicago truncatula cD... 241 1e-062
gb|AL368953.1|AL368953 MtBA27H12R1 MtBA Medicago truncatula... 235 8e-061
gb|BE202442.1|BE202442 EST392891 KV1 Medicago truncatula cD... 219 5e-056
emb|CR342640.1| mte1-75O10RM1 BAC end, cultivar Jemalong A1... 203 3e-051
emb|CR301430.1| mte1-2C20FM1 BAC end, cultivar Jemalong A17... 155 8e-037
gb|CG939621.1|CG939621 MBEIS83TF mth2 Medicago truncatula g... 97 8e-024
>gb|CG962262.1|CG962262 MBEEP40TRB mth2 Medicago truncatula genomic clone 39H8, DNA
sequence
Length = 911
Score = 313 bits (678), Expect = 2e-084
Identities = 118/135 (87%), Positives = 126/135 (93%)
Frame = +2 / +1
Query: 2 VYILATQREKWGDKAYLENGYYLHGYWGILVDRYEEMLENYKPGLGDHRWPLVTHFVGCK 181
VY+LA ++EKWGDK YLENGYYLHGYWGILVDRYEEM+ENY PG GDHRWPLVTHFVGCK
Sbjct: 328 VYLLAKEKEKWGDKVYLENGYYLHGYWGILVDRYEEMIENYHPGFGDHRWPLVTHFVGCK 507
Query: 182 PCGKFGDYPVERCLKNMDRAFNFGDNQILQMYGFTHKSLASRRVKRIRNETSNPLETKDE 361
PCGKFGDYPVERCLK MDRAFNFGDNQILQ+YGFTHKSL SRRV+RIRNETSNPLE KDE
Sbjct: 508 PCGKFGDYPVERCLKQMDRAFNFGDNQILQIYGFTHKSLVSRRVRRIRNETSNPLEVKDE 687
Query: 362 LGLLHPAFKAVKTST 406
LGLLHP FKAV+ S+
Sbjct: 688 LGLLHPTFKAVEVSS 732
Score = 142 bits (304), Expect = 8e-033
Identities = 67/91 (73%), Positives = 71/91 (78%)
Frame = -1 / -1
Query: 285 VNPYICKIWLSPKLNARSIFLRHRSTG*SPNLPHGLHPTKCVTSGHR*SPSPGL*FSSIS 106
VNP IC I LSPKL A SI +H STG*SPN P GLHPTK VT+GH *SP PG *FS IS
Sbjct: 611 VNP*ICNI*LSPKLKALSICFKHLSTG*SPNFPQGLHPTK*VTNGHL*SPKPG**FSIIS 432
Query: 105 SYLSTKIPQ*PWR*YPFSR*ALSPHFSRCVA 13
SYLSTKIPQ*P +* PFSR* LSPHFS +A
Sbjct: 431 SYLSTKIPQ*PCK**PFSR*TLSPHFSFSLA 339
Score = 136 bits (292), Expect = 3e-031
Identities = 55/132 (41%), Positives = 90/132 (68%)
Frame = -3 / -3
Query: 397 LHSLECWMKQPKLILCLKWVAGLVPDPLHSPAC**LVCEPIHLQDLVIPKIECTVHILEA 218
L+S + M+Q K I K + L+P +SP *LV E I+LQ L+I KIE ++H+L+A
Sbjct: 723 LNSFKSRMEQAKFIFNFKRITSLIPYSPNSPTNQ*LVSESINLQYLIITKIESSIHLLQA 544
Query: 217 SLDRVVSKFATRLAPDKVCDEWPPVIAEPWLVILKHLLVPVDQDPPVAVEVVPILKVSLI 38
SL R++++ TRL P K+ +WPP+I + ++IL HLL+ ++Q+PP+ ++V+ ILK++LI
Sbjct: 543 SLHRIITELPTRLTPHKMSHQWPPMITKTRMIILNHLLISINQNPPITMQVITILKINLI 364
Query: 37 TPFLALCREDVH 2
TP L + ++
Sbjct: 363 TPLFLLLSQQIN 328
>gb|CA920561.1|CA920561 EST638279 MTUS Medicago truncatula cDNA clone MTUS-29G1, mRNA
sequence
Length = 647
Score = 288 bits (623), Expect = 8e-077
Identities = 109/124 (87%), Positives = 115/124 (92%)
Frame = +2 / -2
Query: 35 GDKAYLENGYYLHGYWGILVDRYEEMLENYKPGLGDHRWPLVTHFVGCKPCGKFGDYPVE 214
G K YLEN YYLHGYWGILVDRYEEM+ENY PG GDHRWPLVTHFVGCKPCGKFGDYPVE
Sbjct: 634 GGKVYLENHYYLHGYWGILVDRYEEMIENYHPGFGDHRWPLVTHFVGCKPCGKFGDYPVE 455
Query: 215 RCLKNMDRAFNFGDNQILQMYGFTHKSLASRRVKRIRNETSNPLETKDELGLLHPAFKAV 394
RCLK MDRA+NFGDNQILQMYGFTHKSLASRRVKR+RNE+SNPL+ KDELGLLHPAFKA+
Sbjct: 454 RCLKQMDRAYNFGDNQILQMYGFTHKSLASRRVKRVRNESSNPLDVKDELGLLHPAFKAI 275
Query: 395 KTST 406
K T
Sbjct: 274 KLPT 263
Score = 127 bits (271), Expect = 3e-028
Identities = 61/84 (72%), Positives = 63/84 (75%)
Frame = -1 / +3
Query: 285 VNPYICKIWLSPKLNARSIFLRHRSTG*SPNLPHGLHPTKCVTSGHR*SPSPGL*FSSIS 106
V PYIC I LSPKL ARSI +H STG*SPN P GLHPTK VT GH *SP PG *FS IS
Sbjct: 384 VKPYICNI*LSPKL*ARSICFKHLSTG*SPNFPQGLHPTK*VTRGHL*SPKPG**FSIIS 563
Query: 105 SYLSTKIPQ*PWR*YPFSR*ALSP 34
SYLSTKIPQ*P +* FSR* P
Sbjct: 564 SYLSTKIPQ*PCK***FSR*TFPP 635
Score = 113 bits (242), Expect = 3e-024
Identities = 46/124 (37%), Positives = 78/124 (62%)
Frame = -3 / +2
Query: 397 LHSLECWMKQPKLILCLKWVAGLVPDPLHSPAC**LVCEPIHLQDLVIPKIECTVHILEA 218
L S E MKQ K I +K + L+ + H *L+ + IHLQ L+I KI T+H+ +
Sbjct: 272 LDSFESRMKQSKFIFNIKRITTLISNSFHPSTSK*LMSKTIHLQYLIITKIISTIHLFQT 451
Query: 217 SLDRVVSKFATRLAPDKVCDEWPPVIAEPWLVILKHLLVPVDQDPPVAVEVVPILKVSLI 38
L+RV++KF TR P K+ ++ P +I + ++IL HL + ++Q+PP+ ++++ ILK++
Sbjct: 452 PLNRVITKFPTRFTPHKMSNKGPSMITKTRMIILNHLFISINQNPPITMQIIMILKINFP 631
Query: 37 TPFL 26
TP +
Sbjct: 632 TPIV 643
>gb|BG647806.1|BG647806 EST509425 HOGA Medicago truncatula cDNA clone pHOGA-17P10 5' end,
mRNA sequence
Length = 470
Score = 254 bits (550), Expect = 9e-067
Identities = 95/107 (88%), Positives = 100/107 (93%)
Frame = +2 / +2
Query: 2 VYILATQREKWGDKAYLENGYYLHGYWGILVDRYEEMLENYKPGLGDHRWPLVTHFVGCK 181
VY+LAT RE+WG K YLEN YYLHGYWGILVDRYEEM+ENY PG GDHRWPLVTHFVGCK
Sbjct: 149 VYLLATGREQWGGKVYLENHYYLHGYWGILVDRYEEMIENYHPGFGDHRWPLVTHFVGCK 328
Query: 182 PCGKFGDYPVERCLKNMDRAFNFGDNQILQMYGFTHKSLASRRVKRI 322
PCGKFGDYPVERCLK MDRA+NFGDNQILQMYGFTHKSLASRRVKR+
Sbjct: 329 PCGKFGDYPVERCLKQMDRAYNFGDNQILQMYGFTHKSLASRRVKRV 469
Score = 133 bits (284), Expect = 4e-030
Identities = 65/91 (71%), Positives = 67/91 (73%)
Frame = -1 / -3
Query: 285 VNPYICKIWLSPKLNARSIFLRHRSTG*SPNLPHGLHPTKCVTSGHR*SPSPGL*FSSIS 106
V PYIC I LSPKL ARSI +H STG*SPN P GLHPTK VT GH *SP PG *FS IS
Sbjct: 432 VKPYICNI*LSPKL*ARSICFKHLSTG*SPNFPQGLHPTK*VTRGHL*SPKPG**FSIIS 253
Query: 105 SYLSTKIPQ*PWR*YPFSR*ALSPHFSRCVA 13
SYLSTKIPQ*P +* FSR* PH S VA
Sbjct: 252 SYLSTKIPQ*PCK***FSR*TFPPHCSLPVA 160
>gb|BE202431.1|BE202431 EST392880 KV1 Medicago truncatula cDNA clone pKV1-2K11, mRNA
sequence
Length = 573
Score = 241 bits (520), Expect = 1e-062
Identities = 92/105 (87%), Positives = 98/105 (93%)
Frame = +2 / +2
Query: 92 VDRYEEMLENYKPGLGDHRWPLVTHFVGCKPCGKFGDYPVERCLKNMDRAFNFGDNQILQ 271
VDRYEEM+ENY PG DHRWPLVTHFVGCKPCGKFGDYPVERCLK MDRA+NFGDNQILQ
Sbjct: 2 VDRYEEMIENYHPGFCDHRWPLVTHFVGCKPCGKFGDYPVERCLKQMDRAYNFGDNQILQ 181
Query: 272 MYGFTHKSLASRRVKRIRNETSNPLETKDELGLLHPAFKAVKTST 406
MYGFTHKSLASRRVKR+RNE+SNPL+ KDELGLLHPAFKA+K T
Sbjct: 182 MYGFTHKSLASRRVKRVRNESSNPLDVKDELGLLHPAFKAIKLPT 316
Score = 103 bits (219), Expect = 4e-021
Identities = 48/65 (73%), Positives = 49/65 (75%)
Frame = -1 / -1
Query: 285 VNPYICKIWLSPKLNARSIFLRHRSTG*SPNLPHGLHPTKCVTSGHR*SPSPGL*FSSIS 106
V PYIC I LSPKL ARSI +H STG*SPN P GLHPTK VT GH *S PG *FS IS
Sbjct: 195 VKPYICNI*LSPKL*ARSICFKHLSTG*SPNFPQGLHPTK*VTRGHL*SQKPG**FSIIS 16
Query: 105 SYLST 91
SYLST
Sbjct: 15 SYLST 1
>gb|AL368953.1|AL368953 MtBA27H12R1 MtBA Medicago truncatula cDNA clone MtBA27H12 T7, mRNA
sequence
Length = 519
Score = 235 bits (507), Expect = 8e-061
Identities = 89/101 (88%), Positives = 95/101 (94%)
Frame = +2 / +3
Query: 104 EEMLENYKPGLGDHRWPLVTHFVGCKPCGKFGDYPVERCLKNMDRAFNFGDNQILQMYGF 283
EEM+ENY PG GDHRWPLVTHFVGCKPCGKFGDYPVERCLK MDRA+NFGDNQILQMYGF
Sbjct: 3 EEMIENYHPGFGDHRWPLVTHFVGCKPCGKFGDYPVERCLKQMDRAYNFGDNQILQMYGF 182
Query: 284 THKSLASRRVKRIRNETSNPLETKDELGLLHPAFKAVKTST 406
THKSLASRRVKR+RNE+SNPL+ KDELGLLHPAFKA+K T
Sbjct: 183 THKSLASRRVKRVRNESSNPLDVKDELGLLHPAFKAIKLPT 305
>gb|BE202442.1|BE202442 EST392891 KV1 Medicago truncatula cDNA clone pKV1-2K23, mRNA
sequence
Length = 551
Score = 219 bits (472), Expect = 5e-056
Identities = 84/98 (85%), Positives = 90/98 (91%)
Frame = +2 / +1
Query: 113 LENYKPGLGDHRWPLVTHFVGCKPCGKFGDYPVERCLKNMDRAFNFGDNQILQMYGFTHK 292
+ENY PG GDHRW LVT FVGCKPCGKFGDYPVERCLK MDRA+NFGDNQILQMYGFTHK
Sbjct: 1 IENYHPGFGDHRWFLVTDFVGCKPCGKFGDYPVERCLKQMDRAYNFGDNQILQMYGFTHK 180
Query: 293 SLASRRVKRIRNETSNPLETKDELGLLHPAFKAVKTST 406
SLASRRVKR+RNE+SNPL+ KDELGLLHPAFKA+K T
Sbjct: 181 SLASRRVKRVRNESSNPLDVKDELGLLHPAFKAIKLPT 294
>emb|CR342640.1| mte1-75O10RM1 BAC end, cultivar Jemalong A17 of Medicago
truncatula, genomic survey sequence
Length = 364
Score = 203 bits (437), Expect = 3e-051
Identities = 73/106 (68%), Positives = 86/106 (81%)
Frame = +2 / +1
Query: 56 NGYYLHGYWGILVDRYEEMLENYKPGLGDHRWPLVTHFVGCKPCGKFGDYPVERCLKNMD 235
N +YLHGYW LVDRYEEM+E Y PGLGD RWP VTHFVGCKPCG +GDYPVERCL +M+
Sbjct: 1 NSFYLHGYWAGLVDRYEEMMEKYHPGLGDERWPFVTHFVGCKPCGSYGDYPVERCLSSME 180
Query: 236 RAFNFGDNQILQMYGFTHKSLASRRVKRIRNETSNPLETKDELGLL 373
RAFNF DNQ+L++YGF H+ L S ++KRIRNET PLE D+ +L
Sbjct: 181 RAFNFADNQVLKLYGFRHRGLLSPKIKRIRNETITPLEFVDQFDIL 318
>emb|CR301430.1| mte1-2C20FM1 BAC end, cultivar Jemalong A17 of Medicago truncatula,
genomic survey sequence
Length = 412
Score = 155 bits (333), Expect = 8e-037
Identities = 52/76 (68%), Positives = 64/76 (84%)
Frame = +2 / +1
Query: 2 VYILATQREKWGDKAYLENGYYLHGYWGILVDRYEEMLENYKPGLGDHRWPLVTHFVGCK 181
+Y+L ++++KW +K +LEN +YLHGYW LVDRYEEM+E Y PGLGD RWP VTHFVGCK
Sbjct: 184 IYLLLSKKDKWMEKVFLENSFYLHGYWAGLVDRYEEMMEKYHPGLGDERWPFVTHFVGCK 363
Query: 182 PCGKFGDYPVERCLKN 229
PCG +GDYPVERCL +
Sbjct: 364 PCGSYGDYPVERCLSS 411
>gb|CG939621.1|CG939621 MBEIS83TF mth2 Medicago truncatula genomic clone 64N21, DNA
sequence
Length = 882
Score = 96.8 bits (205), Expect(3) = 8e-024
Identities = 39/52 (75%), Positives = 41/52 (78%)
Frame = +2 / -3
Query: 158 VTHFVGCKPCGKFGDYPVERCLKNMDRAFNFGDNQILQMYGFTHKSLASRRV 313
VT FVGCKPC K GDY VERCLK MD FNFGDN ILQ+YGFT SL SR+V
Sbjct: 421 VTRFVGCKPCVKCGDYLVERCLKQMDTTFNFGDNHILQIYGFTQ*SLVSRQV 266
Score = 34.5 bits (69), Expect(3) = 8e-024
Identities = 13/24 (54%), Positives = 19/24 (79%)
Frame = +2 / -3
Query: 314 KRIRNETSNPLETKDELGLLHPAF 385
+++ + TSNPLE KDELGLL ++
Sbjct: 274 RQVWSGTSNPLEVKDELGLLSSSY 203
Score = 21.7 bits (41), Expect(3) = 8e-024
Identities = 6/10 (60%), Positives = 8/10 (80%)
Frame = +2 / -1
Query: 119 NYKPGLGDHR 148
+Y PG GDH+
Sbjct: 450 HYHPGFGDHQ 421
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 792,190,285
Number of Sequences: 392609
Number of extensions: 11657407
Number of successful extensions: 45640
Number of sequences better than 1.0e-020: 9
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 24704515220
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)