TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2192679.2.1
(1274 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CF069643.1|CF069643 EST670364 MTUS Medicago truncatula c... 150 5e-036
gb|BG582275.1|BG582275 EST484015 GVN Medicago truncatula cD... 150 1e-035
gb|DW015279.1|DW015279 EST1224240 MTY Medicago truncatula c... 71 5e-026
gb|BQ139915.1|BQ139915 NF026E11PH1F1086 Phoma-infected Medi... 71 1e-021
>gb|CF069643.1|CF069643 EST670364 MTUS Medicago truncatula cDNA clone MTUS-22F12, mRNA
sequence
Length = 818
Score = 150 bits (323), Expect(3) = 5e-036
Identities = 61/141 (43%), Positives = 89/141 (63%)
Frame = +2 / +3
Query: 146 DSTTPSKMRGASSTMPLTVKQVVDAQQSGTGDKGAPFIVNGVEMANIRLVGMVNAKVERT 325
+ ++P+ + S + V ++ S +GD+ + F +NGV++ N+ LVGMV K ER
Sbjct: 60 NDSSPAPTKSRDSQGLIPVTVKQISEASHSGDEKSNFSINGVDLTNVTLVGMVYEKTERN 239
Query: 326 TDVTFTLDDGTGRLDFIRWVNDASDSFETAAIQNGMYIAVIGSLKGLQERKRATAFSIRP 505
TDV F LDDGTGR+ RWVND DS E + NGMY+ V GSLK Q ++ AFS+RP
Sbjct: 240 TDVNFVLDDGTGRIKCRRWVNDTFDSKEMEEVLNGMYVRVYGSLKSFQGVRQLVAFSVRP 419
Query: 506 ITDFNEVTLHFIQCVRMHIEN 568
+T+F+E+ HFI C+ H+ +
Sbjct: 420 VTNFDEIPFHFIDCIHNHLRS 482
Score = 24.0 bits (46), Expect(3) = 5e-036
Identities = 9/15 (60%), Positives = 11/15 (73%)
Frame = +2 / +3
Query: 761 EHGVHVDEVLKRFKL 805
E GVHVDE+ + KL
Sbjct: 672 ETGVHVDELSRELKL 716
Score = 22.1 bits (42), Expect(3) = 5e-036
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = +2 / +3
Query: 788 LKRFKLLPKKQITDAIDYNMDSGRLYSTIDE 880
L R LP +I + ++ G +YSTID+
Sbjct: 696 LSRELKLPLDKIMLLLKTLLEGGVIYSTIDD 788
>gb|BG582275.1|BG582275 EST484015 GVN Medicago truncatula cDNA clone pGVN-69O9 5' end, mRNA
sequence
Length = 772
Score = 150 bits (323), Expect(2) = 1e-035
Identities = 61/141 (43%), Positives = 89/141 (63%)
Frame = +2 / +1
Query: 146 DSTTPSKMRGASSTMPLTVKQVVDAQQSGTGDKGAPFIVNGVEMANIRLVGMVNAKVERT 325
+ ++P+ + S + V ++ S +GD+ + F +NGV++ N+ LVGMV K ER
Sbjct: 79 NDSSPAPTKSRDSQGLIPVTVKQISEASHSGDEKSNFSINGVDLTNVTLVGMVYEKTERN 258
Query: 326 TDVTFTLDDGTGRLDFIRWVNDASDSFETAAIQNGMYIAVIGSLKGLQERKRATAFSIRP 505
TDV F LDDGTGR+ RWVND DS E + NGMY+ V GSLK Q ++ AFS+RP
Sbjct: 259 TDVNFVLDDGTGRIKCRRWVNDTFDSKEMEEVLNGMYVRVYGSLKSFQGVRQLVAFSVRP 438
Query: 506 ITDFNEVTLHFIQCVRMHIEN 568
+T+F+E+ HFI C+ H+ +
Sbjct: 439 VTNFDEIPFHFIDCIHNHLRS 501
Score = 24.9 bits (48), Expect(2) = 1e-035
Identities = 12/26 (46%), Positives = 15/26 (57%)
Frame = +2 / +1
Query: 761 EHGVHVDEVLKRFKLLPKKQITDAID 838
E GVHVDE+ + KL K + A D
Sbjct: 691 ETGVHVDELSRELKLPLDKIMVGAED 768
>gb|DW015279.1|DW015279 EST1224240 MTY Medicago truncatula cDNA clone MTYA315, mRNA
sequence
Length = 917
Score = 70.7 bits (148), Expect(3) = 5e-026
Identities = 28/79 (35%), Positives = 46/79 (58%)
Frame = +2 / +2
Query: 233 TGDKGAPFIVNGVEMANIRLVGMVNAKVERTTDVTFTLDDGTGRLDFIRWVNDASDSFET 412
+ D ++GV + + LVG V K + T+ F LDDGTG ++ +W+++ +D+
Sbjct: 314 SSDDRTGLTIDGVGVNTVTLVGRVCNKSGQITEFKFVLDDGTGTIECTKWLHEPADAMAV 493
Query: 413 AAIQNGMYIAVIGSLKGLQ 469
+I NGMY+ + G LKG Q
Sbjct: 494 ESILNGMYVRLYGQLKGFQ 550
Score = 51.9 bits (107), Expect(3) = 5e-026
Identities = 17/35 (48%), Positives = 26/35 (74%)
Frame = +2 / +2
Query: 476 KRATAFSIRPITDFNEVTLHFIQCVRMHIENIELK 580
K + FS+RP+TDFNE+ HFI+C+ +H+ N L+
Sbjct: 554 KTLSIFSLRPVTDFNEIASHFIECIYVHLYNSRLQ 658
Score = 35.0 bits (70), Expect(3) = 5e-026
Identities = 15/42 (35%), Positives = 22/42 (52%)
Frame = +2 / +2
Query: 122 TSSQNAAADSTTPSKMRGASSTMPLTVKQVVDAQQSGTGDKG 247
+ + S TPSK R + + +PLT+KQ+ A QS G
Sbjct: 209 SQTNQGGESSLTPSKNRESQTLLPLTIKQINHAPQSSDDRTG 334
>gb|BQ139915.1|BQ139915 NF026E11PH1F1086 Phoma-infected Medicago truncatula cDNA clone
NF026E11PH 5', mRNA sequence
Length = 627
Score = 70.7 bits (148), Expect(3) = 1e-021
Identities = 28/79 (35%), Positives = 46/79 (58%)
Frame = +2 / +3
Query: 233 TGDKGAPFIVNGVEMANIRLVGMVNAKVERTTDVTFTLDDGTGRLDFIRWVNDASDSFET 412
+ D ++GV + + LVG V K + T+ F LDDGTG ++ +W+++ +D+
Sbjct: 297 SSDDRTGLTIDGVGVNTVTLVGRVCNKSGQITEFKFVLDDGTGTIECTKWLHEPADAMAV 476
Query: 413 AAIQNGMYIAVIGSLKGLQ 469
+I NGMY+ + G LKG Q
Sbjct: 477 ESILNGMYVRLYGQLKGFQ 533
Score = 37.3 bits (75), Expect(3) = 1e-021
Identities = 10/20 (50%), Positives = 16/20 (80%)
Frame = +2 / +1
Query: 503 PITDFNEVTLHFIQCVRMHI 562
P+TDFNE+ HF +C+ +H+
Sbjct: 565 PVTDFNEIASHFXECIYVHL 624
Score = 34.5 bits (69), Expect(3) = 1e-021
Identities = 15/42 (35%), Positives = 22/42 (52%)
Frame = +2 / +3
Query: 122 TSSQNAAADSTTPSKMRGASSTMPLTVKQVVDAQQSGTGDKG 247
+ + S TPSK R + + +PLT+KQ+ A QS G
Sbjct: 192 SQTNQGGESSLTPSKNRESQTLLPLTIKQINHALQSSDDRTG 317
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 1,118,758,592
Number of Sequences: 392609
Number of extensions: 14489483
Number of successful extensions: 49271
Number of sequences better than 1.0e-020: 5
length of database: 147,244,331
effective HSP length: 56
effective length of database: 125,258,227
effective search space used: 46095027536
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)