TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2161285.2.2
(798 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ155462.1|BQ155462 NF080G03IR1F1024 Irradiated Medicago... 81 8e-024
gb|AW773989.1|AW773989 EST332975 KV3 Medicago truncatula cD... 81 2e-023
gb|CA919533.1|CA919533 EST637251 MTUS Medicago truncatula c... 81 2e-022
gb|AC161401.2| Medicago truncatula chromosome 7 clone mte1-... 65 4e-021
gb|AC141107.5| Medicago truncatula clone mth2-34a12, comple... 70 4e-021
>gb|BQ155462.1|BQ155462 NF080G03IR1F1024 Irradiated Medicago truncatula cDNA clone
NF080G03IR 5', mRNA sequence
Length = 653
Score = 80.8 bits (170), Expect(3) = 8e-024
Identities = 33/57 (57%), Positives = 42/57 (73%)
Frame = +1 / +2
Query: 283 SVNALGSTATVLTSGNVMDENSFSNPNKVAPVKSQLSNAAEQMQVTLAPHSFTAFDL 453
S+ + GST TVLTS N+MDENSFS P KV P++S L + + M V + PHSFT+FDL
Sbjct: 263 SLQSSGSTKTVLTSTNLMDENSFSQPKKVIPIQSLLQSVGKDMNVIVPPHSFTSFDL 433
Score = 35.9 bits (72), Expect(3) = 8e-024
Identities = 12/26 (46%), Positives = 20/26 (76%)
Frame = +1 / +2
Query: 199 ENSFLRVKVVNFGSDTVNLTVSTSGL 276
+ +++R+K VNFG+ VNL +S +GL
Sbjct: 176 KKNYIRIKAVNFGTSAVNLKISFNGL 253
Score = 32.7 bits (65), Expect(3) = 8e-024
Identities = 15/25 (60%), Positives = 17/25 (68%)
Frame = +2 / +3
Query: 32 GTRTLLSSTPGNTMXLLATGCRXFS 106
GTR L ST ++M LLATGC FS
Sbjct: 3 GTRMQLYSTLFSSMELLATGCNYFS 77
>gb|AW773989.1|AW773989 EST332975 KV3 Medicago truncatula cDNA clone pKV3-16D2, mRNA
sequence
Length = 616
Score = 80.8 bits (170), Expect(3) = 2e-023
Identities = 33/57 (57%), Positives = 42/57 (73%)
Frame = +1 / -2
Query: 283 SVNALGSTATVLTSGNVMDENSFSNPNKVAPVKSQLSNAAEQMQVTLAPHSFTAFDL 453
S+ + GST TVLTS N+MDENSFS P KV P++S L + + M V + PHSFT+FDL
Sbjct: 288 SLQSSGSTKTVLTSTNLMDENSFSQPKKVIPIQSLLQSVGKDMNVIVPPHSFTSFDL 118
Score = 34.1 bits (68), Expect(3) = 2e-023
Identities = 11/26 (42%), Positives = 20/26 (76%)
Frame = +1 / -2
Query: 199 ENSFLRVKVVNFGSDTVNLTVSTSGL 276
+ +++R++ VNFG+ VNL +S +GL
Sbjct: 375 KKNYIRIQAVNFGTSAVNLKISFNGL 298
Score = 33.6 bits (67), Expect(3) = 2e-023
Identities = 18/35 (51%), Positives = 20/35 (57%)
Frame = +2 / -3
Query: 2 HSS*PTMTKRGTRTLLSSTPGNTMXLLATGCRXFS 106
H * M GTR L ST ++M LLATGC FS
Sbjct: 578 HFL*MPMIGGGTRMQLYSTLFSSMELLATGCNYFS 474
>gb|CA919533.1|CA919533 EST637251 MTUS Medicago truncatula cDNA clone MTUS-14H2, mRNA
sequence
Length = 668
Score = 80.8 bits (170), Expect(3) = 2e-022
Identities = 33/57 (57%), Positives = 42/57 (73%)
Frame = +1 / -2
Query: 283 SVNALGSTATVLTSGNVMDENSFSNPNKVAPVKSQLSNAAEQMQVTLAPHSFTAFDL 453
S+ + GST TVLTS N+MDENSFS P KV P++S L + + M V + PHSFT+FDL
Sbjct: 340 SLQSSGSTKTVLTSTNLMDENSFSQPKKVIPIQSLLQSVGKDMNVIVPPHSFTSFDL 170
Score = 35.9 bits (72), Expect(3) = 2e-022
Identities = 12/26 (46%), Positives = 20/26 (76%)
Frame = +1 / -2
Query: 199 ENSFLRVKVVNFGSDTVNLTVSTSGL 276
+ +++R+K VNFG+ VNL +S +GL
Sbjct: 427 KKNYIRIKAVNFGTSAVNLKISFNGL 350
Score = 27.6 bits (54), Expect(3) = 2e-022
Identities = 13/23 (56%), Positives = 15/23 (65%)
Frame = +2 / -3
Query: 38 RTLLSSTPGNTMXLLATGCRXFS 106
R L ST ++M LLATGC FS
Sbjct: 594 RMQLYSTLFSSMELLATGCNYFS 526
>gb|AC161401.2| Medicago truncatula chromosome 7 clone mte1-59b16, *** SEQUENCING IN
PROGRESS ***, 2 ordered pieces
Length = 95675
Score = 65.2 bits (136), Expect(3) = 4e-021
Identities = 28/51 (54%), Positives = 36/51 (70%)
Frame = +1 / +1
Query: 217 VKVVNFGSDTVNLTVSTSGLGASVNALGSTATVLTSGNVMDENSFSNPNKV 369
++VVNFG+ T N +S +GL + V + S+ VLTS N MDENSFS PNKV
Sbjct: 8251 LQVVNFGNATENFMISINGLNSIVQSSNSSMVVLTSSNRMDENSFSEPNKV 8403
Score = 65.2 bits (136), Expect(3) = 4e-021
Identities = 28/51 (54%), Positives = 36/51 (70%)
Frame = +1 / +2
Query: 217 VKVVNFGSDTVNLTVSTSGLGASVNALGSTATVLTSGNVMDENSFSNPNKV 369
++VVNFG+ T N +S +GL + V + S+ VLTS N MDENSFS PNKV
Sbjct: 1754 LQVVNFGNATENFMISINGLNSIVQSSNSSMVVLTSSNRMDENSFSEPNKV 1906
Score = 55.6 bits (115), Expect(3) = 4e-021
Identities = 19/25 (76%), Positives = 22/25 (88%)
Frame = +1 / +2
Query: 31 WNPDAIVFNSWQHYGTPSYWMQTXF 105
W PDAIVFNS+Q+YGTPSYW+Q F
Sbjct: 7925 WIPDAIVFNSYQNYGTPSYWLQQLF 7999
Score = 55.6 bits (115), Expect(3) = 4e-021
Identities = 19/25 (76%), Positives = 22/25 (88%)
Frame = +1 / +3
Query: 31 WNPDAIVFNSWQHYGTPSYWMQTXF 105
W PDAIVFNS+Q+YGTPSYW+Q F
Sbjct: 1428 WIPDAIVFNSYQNYGTPSYWLQQLF 1502
Score = 36.8 bits (74), Expect(3) = 4e-021
Identities = 13/32 (40%), Positives = 22/32 (68%)
Frame = +1 / +1
Query: 364 KVAPVKSQLSNAAEQMQVTLAPHSFTAFDLAL 459
++ P ++ L NA+ M V L P+S T+FDL++
Sbjct: 47914 QIVPQRTALGNASNDMDVRLPPYSVTSFDLSI 48009
Score = 36.8 bits (74), Expect(3) = 4e-021
Identities = 13/32 (40%), Positives = 22/32 (68%)
Frame = +1 / +3
Query: 364 KVAPVKSQLSNAAEQMQVTLAPHSFTAFDLAL 459
++ P ++ L NA+ M V L P+S T+FDL++
Sbjct: 2130 QIVPQRTALGNASNDMDVRLPPYSVTSFDLSI 2225
>gb|AC141107.5| Medicago truncatula clone mth2-34a12, complete sequence
Length = 142659
Score = 69.8 bits (146), Expect(4) = 4e-021
Identities = 27/56 (48%), Positives = 33/56 (58%)
Frame = +1 / +2
Query: 31 WNPDAIVFNSWQHYGTPSYWMQTXFRRVNRAIVHPVNIXXXXXXXXXXXXITWQDA 198
WNPDAIVFNS+Q YGTPSYWMQ F N A + ++ ITWQ++
Sbjct: 58691 WNPDAIVFNSFQLYGTPSYWMQLFFSESNGATLLNSSLQTTASNSLVASAITWQNS 58858
Score = 47.3 bits (97), Expect(4) = 4e-021
Identities = 20/29 (68%), Positives = 23/29 (79%)
Frame = +1 / +2
Query: 283 SVNALGSTATVLTSGNVMDENSFSNPNKV 369
S+ + GST TVLTS N+MDENSFS P KV
Sbjct: 59318 SLQSSGSTKTVLTSTNLMDENSFSQPKKV 59404
Score = 38.6 bits (78), Expect(4) = 4e-021
Identities = 14/30 (46%), Positives = 21/30 (70%)
Frame = +1 / +1
Query: 364 KVAPVKSQLSNAAEQMQVTLAPHSFTAFDL 453
+V P++S L + + M V + PHSFT+FDL
Sbjct: 59536 QVIPIQSLLQSVGKDMNVIVPPHSFTSFDL 59625
Score = 27.6 bits (54), Expect(4) = 4e-021
Identities = 10/20 (50%), Positives = 15/20 (75%)
Frame = +1 / +2
Query: 217 VKVVNFGSDTVNLTVSTSGL 276
++ VNFG+ VNL +S +GL
Sbjct: 59249 LQAVNFGTSAVNLKISFNGL 59308
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 791,442,505
Number of Sequences: 392609
Number of extensions: 10838635
Number of successful extensions: 58451
Number of sequences better than 1.0e-020: 7
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 26595166895
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)