TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2161272.2.19
(1410 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG455193.1|BG455193 NF100G04PL1F1034 Phosphate starved l... 62 1e-034
gb|BG453428.1|BG453428 NF091G11LF1F1085 Developing leaf Med... 58 2e-031
gb|AL379968.1|AL379968 MtBB48E05F1 MtBB Medicago truncatula... 62 1e-028
gb|CA917715.1|CA917715 EST641862 GPOD Medicago truncatula c... 66 1e-026
gb|CA990029.1|CA990029 EST643537 GESD Medicago truncatula c... 69 1e-024
gb|BF648216.1|BF648216 NF045D01EC1F1012 Elicited cell cultu... 63 3e-023
>gb|BG455193.1|BG455193 NF100G04PL1F1034 Phosphate starved leaf Medicago truncatula cDNA
clone NF100G04PL 5', mRNA sequence
Length = 654
Score = 61.6 bits (128), Expect(4) = 1e-034
Identities = 25/59 (42%), Positives = 36/59 (61%)
Frame = -3 / +2
Query: 859 AGQKIVFHIEKGCNPNYLAVLVKYVADDGDIVLMEIQDKLSAEWKPMKLSWGAIWRMDT 683
AG+ I F + +G P +L++LV++ DGDI M IQ+ S+EW M WGA W + T
Sbjct: 320 AGKNIAFQVNEGSTPFWLSLLVEFEDGDGDISSMHIQEAGSSEWLQMNHLWGANWNIVT 496
Score = 50.1 bits (103), Expect(4) = 1e-034
Identities = 19/49 (38%), Positives = 27/49 (55%)
Frame = -3 / +2
Query: 1150 VNLPPYSGMTACGNVPIFKDGKGCGSCYEVRCKEKPECSGNPVTVYITD 1004
V++ P ++K G+GCG CY+V+C + CS VTV ITD
Sbjct: 29 VDVKPLRARVGAVGPVLYKKGEGCGECYKVKCLDHTICSKRAVTVIITD 175
Score = 46.4 bits (95), Expect(4) = 1e-034
Identities = 15/27 (55%), Positives = 24/27 (88%)
Frame = -3 / +2
Query: 673 LKGPFSIRLTSESGKKVIAKDVIPANW 593
L+GPFS++L++ +GK + AKDVIP++W
Sbjct: 503 LRGPFSVKLSTSTGKSLTAKDVIPSHW 583
Score = 46.4 bits (95), Expect(4) = 1e-034
Identities = 19/38 (50%), Positives = 25/38 (65%)
Frame = -3 / +2
Query: 976 HFDLSGKAFGSLAKPGLNDKIRHCGIMDVEFRRVRCKY 863
HFDLSG AFG +A G N ++R+ G + V +RR C Y
Sbjct: 206 HFDLSGAAFGHMAVHGENGQLRNRGEIQVLYRRTTCVY 319
>gb|BG453428.1|BG453428 NF091G11LF1F1085 Developing leaf Medicago truncatula cDNA clone
NF091G11LF 5', mRNA sequence
Length = 638
Score = 58.3 bits (121), Expect(4) = 2e-031
Identities = 22/41 (53%), Positives = 31/41 (75%)
Frame = -3 / +3
Query: 985 APYHFDLSGKAFGSLAKPGLNDKIRHCGIMDVEFRRVRCKY 863
A YHFDLSG AFGS+A G ++++R+ G + +E RRV+C Y
Sbjct: 18 AEYHFDLSGSAFGSMAVSGEDEELRNAGKIVIEHRRVQCNY 140
Score = 51.0 bits (105), Expect(4) = 2e-031
Identities = 19/40 (47%), Positives = 24/40 (60%)
Frame = -3 / +3
Query: 739 SAEWKPMKLSWGAIWRMDTAKALKGPFSIRLTSESGKKVI 620
S+ W M+ SWGA+W+ D LK P SIRLT+ K I
Sbjct: 264 SSSWDTMQQSWGAVWKFDKGAPLKAPISIRLTTLKSGKTI 383
Score = 44.1 bits (90), Expect(4) = 2e-031
Identities = 15/41 (36%), Positives = 25/41 (60%)
Frame = -3 / +3
Query: 856 GQKIVFHIEKGCNPNYLAVLVKYVADDGDIVLMEIQDKLSA 734
G I FH++ G N Y A L++Y DGD+ +E+++ L +
Sbjct: 144 GSTIAFHVDSGSNQEYFAALIEYEEGDGDLNKVELKEALDS 266
Score = 40.5 bits (82), Expect(4) = 2e-031
Identities = 15/30 (50%), Positives = 20/30 (66%)
Frame = -3 / +3
Query: 646 TSESGKKVIAKDVIPANWRPDAVYTSNVQF 557
T +SGK ++A +VIPA W+P Y S V F
Sbjct: 360 TLKSGKTIVAHNVIPAGWKPGQTYRSIVNF 449
>gb|AL379968.1|AL379968 MtBB48E05F1 MtBB Medicago truncatula cDNA clone MtBB48E05 T3, mRNA
sequence
Length = 476
Score = 61.6 bits (128), Expect(4) = 1e-028
Identities = 25/59 (42%), Positives = 36/59 (61%)
Frame = -3 / +2
Query: 859 AGQKIVFHIEKGCNPNYLAVLVKYVADDGDIVLMEIQDKLSAEWKPMKLSWGAIWRMDT 683
AG+ I F + +G P +L++LV++ DGDI M IQ+ S+EW M WGA W + T
Sbjct: 248 AGKNIAFQVNEGSTPFWLSLLVEFEDGDGDISSMHIQEAGSSEWLQMNHLWGANWNIVT 424
Score = 49.2 bits (101), Expect(4) = 1e-028
Identities = 17/33 (51%), Positives = 23/33 (69%)
Frame = -3 / +2
Query: 1102 IFKDGKGCGSCYEVRCKEKPECSGNPVTVYITD 1004
++K G+GCG CY+V+C + CS VTV ITD
Sbjct: 5 LYKKGEGCGECYKVKCLDHTICSKRAVTVIITD 103
Score = 46.4 bits (95), Expect(4) = 1e-028
Identities = 19/38 (50%), Positives = 25/38 (65%)
Frame = -3 / +2
Query: 976 HFDLSGKAFGSLAKPGLNDKIRHCGIMDVEFRRVRCKY 863
HFDLSG AFG +A G N ++R+ G + V +RR C Y
Sbjct: 134 HFDLSGAAFGHMAVHGENGQLRNRGEIQVLYRRTTCVY 247
Score = 26.7 bits (52), Expect(4) = 1e-028
Identities = 8/15 (53%), Positives = 14/15 (93%)
Frame = -3 / +2
Query: 673 LKGPFSIRLTSESGK 629
L+GPFS++L++ +GK
Sbjct: 431 LRGPFSVKLSTSTGK 475
>gb|CA917715.1|CA917715 EST641862 GPOD Medicago truncatula cDNA clone GPOD-37E9, mRNA
sequence
Length = 769
Score = 65.7 bits (137), Expect(3) = 1e-026
Identities = 26/57 (45%), Positives = 36/57 (63%)
Frame = -3 / +2
Query: 859 AGQKIVFHIEKGCNPNYLAVLVKYVADDGDIVLMEIQDKLSAEWKPMKLSWGAIWRM 689
AG+ I F + +G P +L++LV++ DGDI M IQ+ S+EW M WGAIW M
Sbjct: 572 AGKNIAFQVNEGSTPFWLSLLVEFEDGDGDISSMHIQEAGSSEWLQMNHLWGAIWNM 742
Score = 47.8 bits (98), Expect(3) = 1e-026
Identities = 18/49 (36%), Positives = 26/49 (53%)
Frame = -3 / +2
Query: 1150 VNLPPYSGMTACGNVPIFKDGKGCGSCYEVRCKEKPECSGNPVTVYITD 1004
V++ P ++ G+GCG CY+V+C + CS VTV ITD
Sbjct: 281 VDVKPLRARVGAVGPVLYNKGEGCGECYKVKCLDHTICSKRAVTVIITD 427
Score = 46.4 bits (95), Expect(3) = 1e-026
Identities = 19/38 (50%), Positives = 25/38 (65%)
Frame = -3 / +2
Query: 976 HFDLSGKAFGSLAKPGLNDKIRHCGIMDVEFRRVRCKY 863
HFDLSG AFG +A G N ++R+ G + V +RR C Y
Sbjct: 458 HFDLSGAAFGHMAVHGENGQLRNRGEIQVLYRRTTCVY 571
>gb|CA990029.1|CA990029 EST643537 GESD Medicago truncatula cDNA clone GESD-27G8, mRNA
sequence
Length = 801
Score = 69.3 bits (145), Expect(3) = 1e-024
Identities = 30/93 (32%), Positives = 52/93 (55%)
Frame = -3 / +1
Query: 973 FDLSGKAFGSLAKPGLNDKIRHCGIMDVEFRRVRCKYPAGQKIVFHIEKGCNPNYLAVLV 794
F LS +AF ++A+ G++ +I GI+D+E++RV C+Y V E P+YLA+
Sbjct: 250 FVLSSRAFTAMAQKGMSQQILKLGIVDIEYKRVPCEYKKQNLAVRVEESSKKPDYLAIKF 429
Query: 793 KYVADDGDIVLMEIQDKLSAEWKPMKLSWGAIW 695
Y +IV +++ S+ W + + GA+W
Sbjct: 430 LYQGGQTEIVGVDVAQVGSSNWSFLSRNHGAVW 528
Score = 51.5 bits (106), Expect(3) = 1e-024
Identities = 18/45 (40%), Positives = 28/45 (62%)
Frame = -3 / +1
Query: 1129 GMTACGNVPIFKDGKGCGSCYEVRCKEKPECSGNPVTVYITDMNY 995
G A G +F +G GCG+C++VRCK + C+ V +TD+N+
Sbjct: 100 GHLAAGVSSLFYNGAGCGACFQVRCKNQAICTKEGTKVVLTDLNH 234
Score = 32.2 bits (64), Expect(3) = 1e-024
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = -3 / +1
Query: 634 GKKVIAKDVIPANWRPDAVYTSNVQ 560
GK + AK V+PA+W+ +Y S++Q
Sbjct: 592 GKWLWAKKVLPADWKNGVIYDSDIQ 666
>gb|BF648216.1|BF648216 NF045D01EC1F1012 Elicited cell culture Medicago truncatula cDNA
clone NF045D01EC 5', mRNA sequence
Length = 596
Score = 62.9 bits (131), Expect(3) = 3e-023
Identities = 21/40 (52%), Positives = 32/40 (80%)
Frame = -3 / +3
Query: 673 LKGPFSIRLTSESGKKVIAKDVIPANWRPDAVYTSNVQFY 554
L+GPFS++L+S +G+ + A+DV+P+NW P A YTS + FY
Sbjct: 291 LRGPFSVKLSSSTGRTLSARDVVPSNWVPKATYTSRLNFY 410
Score = 60.6 bits (126), Expect(3) = 3e-023
Identities = 23/54 (42%), Positives = 35/54 (64%)
Frame = -3 / +3
Query: 856 GQKIVFHIEKGCNPNYLAVLVKYVADDGDIVLMEIQDKLSAEWKPMKLSWGAIW 695
G+KI FH+ +G P +L++LV++ +GDI M I++ S+EW M WGA W
Sbjct: 111 GKKIAFHVNEGSTPFWLSLLVEFEDAEGDIGTMHIRENGSSEWLQMNHLWGANW 272
Score = 24.9 bits (48), Expect(3) = 3e-023
Identities = 9/19 (47%), Positives = 13/19 (68%)
Frame = -3 / +3
Query: 916 IRHCGIMDVEFRRVRCKYP 860
+R+ G + V +RR CKYP
Sbjct: 54 LRNRGQIPVIYRRTPCKYP 110
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 1,252,840,653
Number of Sequences: 392609
Number of extensions: 17332076
Number of successful extensions: 68560
Number of sequences better than 1.0e-020: 9
length of database: 147,244,331
effective HSP length: 56
effective length of database: 125,258,227
effective search space used: 51731647751
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)