TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2161272.2.15
(797 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG455193.1|BG455193 NF100G04PL1F1034 Phosphate starved l... 63 4e-034
gb|BG453428.1|BG453428 NF091G11LF1F1085 Developing leaf Med... 59 2e-031
gb|AL379968.1|AL379968 MtBB48E05F1 MtBB Medicago truncatula... 63 2e-028
gb|CA917715.1|CA917715 EST641862 GPOD Medicago truncatula c... 67 7e-027
gb|BF648216.1|BF648216 NF045D01EC1F1012 Elicited cell cultu... 62 4e-024
gb|CA990029.1|CA990029 EST643537 GESD Medicago truncatula c... 67 5e-022
gb|AW687498.2|AW687498 NF010C04RT1F1033 Developing root Med... 62 6e-021
gb|AL379969.1|AL379969 MtBB48E05R1 MtBB Medicago truncatula... 63 6e-021
gb|BG645484.1|BG645484 EST507103 KV3 Medicago truncatula cD... 62 8e-021
>gb|BG455193.1|BG455193 NF100G04PL1F1034 Phosphate starved leaf Medicago truncatula cDNA
clone NF100G04PL 5', mRNA sequence
Length = 654
Score = 62.9 bits (131), Expect(4) = 4e-034
Identities = 26/59 (44%), Positives = 36/59 (61%)
Frame = +2 / +2
Query: 233 AGQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAEWKPMKLSWGAIWRMDT 409
AG+ I F + +G P +L++LV+F DGDI M IQ+ S+EW M WGA W + T
Sbjct: 320 AGKNIAFQVNEGSTPFWLSLLVEFEDGDGDISSMHIQEAGSSEWLQMNHLWGANWNIVT 496
Score = 47.3 bits (97), Expect(4) = 4e-034
Identities = 20/38 (52%), Positives = 25/38 (65%)
Frame = +2 / +2
Query: 116 HFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKY 229
HFDLSG AFG +A G N +LR+ G + V +RR C Y
Sbjct: 206 HFDLSGAAFGHMAVHGENGQLRNRGEIQVLYRRTTCVY 319
Score = 46.0 bits (94), Expect(4) = 4e-034
Identities = 14/27 (51%), Positives = 24/27 (88%)
Frame = +2 / +2
Query: 419 LKGPFSIRLTSESGKKVIAKDIIPANW 499
L+GPFS++L++ +GK + AKD+IP++W
Sbjct: 503 LRGPFSVKLSTSTGKSLTAKDVIPSHW 583
Score = 45.5 bits (93), Expect(4) = 4e-034
Identities = 16/29 (55%), Positives = 20/29 (68%)
Frame = +2 / +2
Query: 2 GKGCGSCYEVRCKEKPECSGNPVTVFITD 88
G+GCG CY+V+C + CS VTV ITD
Sbjct: 89 GEGCGECYKVKCLDHTICSKRAVTVIITD 175
>gb|BG453428.1|BG453428 NF091G11LF1F1085 Developing leaf Medicago truncatula cDNA clone
NF091G11LF 5', mRNA sequence
Length = 638
Score = 59.3 bits (123), Expect(4) = 2e-031
Identities = 23/41 (56%), Positives = 31/41 (75%)
Frame = +2 / +3
Query: 107 APYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKY 229
A YHFDLSG AFGS+A G +++LR+ G + +E RRV+C Y
Sbjct: 18 AEYHFDLSGSAFGSMAVSGEDEELRNAGKIVIEHRRVQCNY 140
Score = 51.0 bits (105), Expect(4) = 2e-031
Identities = 19/40 (47%), Positives = 24/40 (60%)
Frame = +2 / +3
Query: 353 SAEWKPMKLSWGAIWRMDTAKALKGPFSIRLTSESGKKVI 472
S+ W M+ SWGA+W+ D LK P SIRLT+ K I
Sbjct: 264 SSSWDTMQQSWGAVWKFDKGAPLKAPISIRLTTLKSGKTI 383
Score = 42.3 bits (86), Expect(4) = 2e-031
Identities = 14/41 (34%), Positives = 25/41 (60%)
Frame = +2 / +3
Query: 236 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSA 358
G I FH++ G N Y A L+++ DGD+ +E+++ L +
Sbjct: 144 GSTIAFHVDSGSNQEYFAALIEYEEGDGDLNKVELKEALDS 266
Score = 40.0 bits (81), Expect(4) = 2e-031
Identities = 14/30 (46%), Positives = 20/30 (66%)
Frame = +2 / +3
Query: 446 TSESGKKVIAKDIIPANWRPDAVYTSNVQF 535
T +SGK ++A ++IPA W+P Y S V F
Sbjct: 360 TLKSGKTIVAHNVIPAGWKPGQTYRSIVNF 449
>gb|AL379968.1|AL379968 MtBB48E05F1 MtBB Medicago truncatula cDNA clone MtBB48E05 T3, mRNA
sequence
Length = 476
Score = 62.9 bits (131), Expect(4) = 2e-028
Identities = 26/59 (44%), Positives = 36/59 (61%)
Frame = +2 / +2
Query: 233 AGQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAEWKPMKLSWGAIWRMDT 409
AG+ I F + +G P +L++LV+F DGDI M IQ+ S+EW M WGA W + T
Sbjct: 248 AGKNIAFQVNEGSTPFWLSLLVEFEDGDGDISSMHIQEAGSSEWLQMNHLWGANWNIVT 424
Score = 47.3 bits (97), Expect(4) = 2e-028
Identities = 20/38 (52%), Positives = 25/38 (65%)
Frame = +2 / +2
Query: 116 HFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKY 229
HFDLSG AFG +A G N +LR+ G + V +RR C Y
Sbjct: 134 HFDLSGAAFGHMAVHGENGQLRNRGEIQVLYRRTTCVY 247
Score = 45.5 bits (93), Expect(4) = 2e-028
Identities = 16/29 (55%), Positives = 20/29 (68%)
Frame = +2 / +2
Query: 2 GKGCGSCYEVRCKEKPECSGNPVTVFITD 88
G+GCG CY+V+C + CS VTV ITD
Sbjct: 17 GEGCGECYKVKCLDHTICSKRAVTVIITD 103
Score = 26.7 bits (52), Expect(4) = 2e-028
Identities = 8/15 (53%), Positives = 14/15 (93%)
Frame = +2 / +2
Query: 419 LKGPFSIRLTSESGK 463
L+GPFS++L++ +GK
Sbjct: 431 LRGPFSVKLSTSTGK 475
>gb|CA917715.1|CA917715 EST641862 GPOD Medicago truncatula cDNA clone GPOD-37E9, mRNA
sequence
Length = 769
Score = 67.0 bits (140), Expect(3) = 7e-027
Identities = 27/57 (47%), Positives = 36/57 (63%)
Frame = +2 / +2
Query: 233 AGQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAEWKPMKLSWGAIWRM 403
AG+ I F + +G P +L++LV+F DGDI M IQ+ S+EW M WGAIW M
Sbjct: 572 AGKNIAFQVNEGSTPFWLSLLVEFEDGDGDISSMHIQEAGSSEWLQMNHLWGAIWNM 742
Score = 47.3 bits (97), Expect(3) = 7e-027
Identities = 20/38 (52%), Positives = 25/38 (65%)
Frame = +2 / +2
Query: 116 HFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKY 229
HFDLSG AFG +A G N +LR+ G + V +RR C Y
Sbjct: 458 HFDLSGAAFGHMAVHGENGQLRNRGEIQVLYRRTTCVY 571
Score = 45.5 bits (93), Expect(3) = 7e-027
Identities = 16/29 (55%), Positives = 20/29 (68%)
Frame = +2 / +2
Query: 2 GKGCGSCYEVRCKEKPECSGNPVTVFITD 88
G+GCG CY+V+C + CS VTV ITD
Sbjct: 341 GEGCGECYKVKCLDHTICSKRAVTVIITD 427
>gb|BF648216.1|BF648216 NF045D01EC1F1012 Elicited cell culture Medicago truncatula cDNA
clone NF045D01EC 5', mRNA sequence
Length = 596
Score = 62.5 bits (130), Expect(3) = 4e-024
Identities = 20/40 (50%), Positives = 32/40 (80%)
Frame = +2 / +3
Query: 419 LKGPFSIRLTSESGKKVIAKDIIPANWRPDAVYTSNVQFY 538
L+GPFS++L+S +G+ + A+D++P+NW P A YTS + FY
Sbjct: 291 LRGPFSVKLSSSTGRTLSARDVVPSNWVPKATYTSRLNFY 410
Score = 62.0 bits (129), Expect(3) = 4e-024
Identities = 24/54 (44%), Positives = 35/54 (64%)
Frame = +2 / +3
Query: 236 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAEWKPMKLSWGAIW 397
G+KI FH+ +G P +L++LV+F +GDI M I++ S+EW M WGA W
Sbjct: 111 GKKIAFHVNEGSTPFWLSLLVEFEDAEGDIGTMHIRENGSSEWLQMNHLWGANW 272
Score = 25.8 bits (50), Expect(3) = 4e-024
Identities = 10/19 (52%), Positives = 13/19 (68%)
Frame = +2 / +3
Query: 176 LRHCGIMDVEFRRVRCKYP 232
LR+ G + V +RR CKYP
Sbjct: 54 LRNRGQIPVIYRRTPCKYP 110
>gb|CA990029.1|CA990029 EST643537 GESD Medicago truncatula cDNA clone GESD-27G8, mRNA
sequence
Length = 801
Score = 66.6 bits (139), Expect(3) = 5e-022
Identities = 28/93 (30%), Positives = 52/93 (55%)
Frame = +2 / +1
Query: 119 FDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYPAGQKIVFHIEKGCNPNYLAVLV 298
F LS +AF ++A+ G++ ++ GI+D+E++RV C+Y V E P+YLA+
Sbjct: 250 FVLSSRAFTAMAQKGMSQQILKLGIVDIEYKRVPCEYKKQNLAVRVEESSKKPDYLAIKF 429
Query: 299 KFVADDGDIVLMEIQDKLSAEWKPMKLSWGAIW 397
+ +IV +++ S+ W + + GA+W
Sbjct: 430 LYQGGQTEIVGVDVAQVGSSNWSFLSRNHGAVW 528
Score = 45.1 bits (92), Expect(3) = 5e-022
Identities = 14/32 (43%), Positives = 22/32 (68%)
Frame = +2 / +1
Query: 2 GKGCGSCYEVRCKEKPECSGNPVTVFITDMNY 97
G GCG+C++VRCK + C+ V +TD+N+
Sbjct: 139 GAGCGACFQVRCKNQAICTKEGTKVVLTDLNH 234
Score = 31.8 bits (63), Expect(3) = 5e-022
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +2 / +1
Query: 458 GKKVIAKDIIPANWRPDAVYTSNVQ 532
GK + AK ++PA+W+ +Y S++Q
Sbjct: 592 GKWLWAKKVLPADWKNGVIYDSDIQ 666
>gb|AW687498.2|AW687498 NF010C04RT1F1033 Developing root Medicago truncatula cDNA clone
NF010C04RT 5', mRNA sequence
Length = 522
Score = 61.6 bits (128), Expect(2) = 6e-021
Identities = 25/59 (42%), Positives = 36/59 (61%)
Frame = +2 / +2
Query: 233 AGQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAEWKPMKLSWGAIWRMDT 409
AG+ + F + +G P +L++LV+F DGDI M IQ+ S+EW M WGA W + T
Sbjct: 173 AGKNLAFQVNEGSTPYWLSLLVEFEDGDGDISSMYIQEAGSSEWLQMNHLWGANWNIVT 349
Score = 60.6 bits (126), Expect(2) = 6e-021
Identities = 19/39 (48%), Positives = 30/39 (76%)
Frame = +2 / +2
Query: 419 LKGPFSIRLTSESGKKVIAKDIIPANWRPDAVYTSNVQF 535
L+GPFS++L++ +GK + KD+IP+NW P + YTS + F
Sbjct: 356 LRGPFSVKLSTSTGKSITVKDVIPSNWSPKSTYTSRLNF 472
>gb|AL379969.1|AL379969 MtBB48E05R1 MtBB Medicago truncatula cDNA clone MtBB48E05 T7, mRNA
sequence
Length = 488
Score = 62.9 bits (131), Expect(2) = 6e-021
Identities = 26/59 (44%), Positives = 36/59 (61%)
Frame = +2 / +2
Query: 233 AGQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAEWKPMKLSWGAIWRMDT 409
AG+ I F + +G P +L++LV+F DGDI M IQ+ S+EW M WGA W + T
Sbjct: 44 AGKNIAFQVNEGSTPFWLSLLVEFEDGDGDISSMHIQEAGSSEWLQMNHLWGANWNIVT 220
Score = 59.3 bits (123), Expect(2) = 6e-021
Identities = 19/39 (48%), Positives = 31/39 (79%)
Frame = +2 / +2
Query: 419 LKGPFSIRLTSESGKKVIAKDIIPANWRPDAVYTSNVQF 535
L+GPFS++L++ +GK + AKD+IP++W P + YTS + F
Sbjct: 227 LRGPFSVKLSTSTGKSLTAKDVIPSHWSPKSTYTSRLNF 343
>gb|BG645484.1|BG645484 EST507103 KV3 Medicago truncatula cDNA clone pKV3-46K12 5' end,
mRNA sequence
Length = 742
Score = 62.5 bits (130), Expect(2) = 8e-021
Identities = 20/40 (50%), Positives = 32/40 (80%)
Frame = +2 / -3
Query: 419 LKGPFSIRLTSESGKKVIAKDIIPANWRPDAVYTSNVQFY 538
L+GPFS++L+S +G+ + A+D++P+NW P A YTS + FY
Sbjct: 503 LRGPFSVKLSSSTGRTLSARDVVPSNWVPKATYTSRLNFY 384
Score = 59.3 bits (123), Expect(2) = 8e-021
Identities = 23/53 (43%), Positives = 34/53 (64%)
Frame = +2 / -3
Query: 239 QKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAEWKPMKLSWGAIW 397
+KI FH+ +G P +L++LV+F +GDI M I++ S+EW M WGA W
Sbjct: 680 KKIAFHVNEGSTPFWLSLLVEFEDAEGDIGTMHIRENGSSEWLQMNHLWGANW 522
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 775,730,607
Number of Sequences: 392609
Number of extensions: 10477348
Number of successful extensions: 48164
Number of sequences better than 1.0e-020: 12
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 26595166895
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)