TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1716368.2.1
(1356 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA917715.1|CA917715 EST641862 GPOD Medicago truncatula c... 128 4e-050
gb|BG455193.1|BG455193 NF100G04PL1F1034 Phosphate starved l... 130 3e-048
gb|AL379968.1|AL379968 MtBB48E05F1 MtBB Medicago truncatula... 130 1e-041
gb|BG453428.1|BG453428 NF091G11LF1F1085 Developing leaf Med... 96 4e-036
gb|BG448066.1|BG448066 NF105C08EC1F1056 Elicited cell cultu... 98 6e-036
gb|BG452295.1|BG452295 NF085E05LF1F1039 Developing leaf Med... 94 8e-033
gb|AC148226.7| Medicago truncatula clone mth2-5i20, complet... 60 1e-031
gb|AC174309.3| Medicago truncatula clone mth2-139k12, WORKI... 60 3e-031
gb|AL379969.1|AL379969 MtBB48E05R1 MtBB Medicago truncatula... 104 7e-028
gb|AW687498.2|AW687498 NF010C04RT1F1033 Developing root Med... 105 2e-027
gb|BF648216.1|BF648216 NF045D01EC1F1012 Elicited cell cultu... 98 1e-026
>gb|CA917715.1|CA917715 EST641862 GPOD Medicago truncatula cDNA clone GPOD-37E9, mRNA
sequence
Length = 769
Score = 128 bits (274), Expect(2) = 4e-050
Identities = 48/100 (48%), Positives = 67/100 (67%)
Frame = -1 / +2
Query: 768 PGEVEHFDMSGTAMGAMARPGMADKLRAGGVLRILYRRVPCKYTGVNIAFKVDQGANPYY 589
P + HFD+SG A G MA G +LR G +++LYRR C Y G NIAF+V++G+ P++
Sbjct: 443 PSDRTHFDLSGAAFGHMAVHGENGQLRNRGEIQVLYRRTTCVYAGKNIAFQVNEGSTPFW 622
Query: 588 FDVLIEFEDDDGDLSAVDLMEAGSGVWTPMAHNWGATWRL 469
+L+EFED DGD+S++ + EAGS W M H WGA W +
Sbjct: 623 LSLLVEFEDGDGDISSMHIQEAGSSEWLQMNHLWGAIWNM 742
Score = 92.7 bits (196), Expect(2) = 4e-050
Identities = 35/67 (52%), Positives = 44/67 (65%)
Frame = -1 / +2
Query: 978 GDGSDGGACGYQTAVGKKPFDSMIAAGSTPLYRGGEGCGACYEVKCTTNAACSGQPVTIV 799
GDGS GGACGY + V KP + + A LY GEGCG CY+VKC + CS + VT++
Sbjct: 239 GDGSRGGACGYGSLVDVKPLRARVGAVGPVLYNKGEGCGECYKVKCLDHTICSKRAVTVI 418
Query: 798 ITDQSPG 778
ITD+ PG
Sbjct: 419 ITDECPG 439
>gb|BG455193.1|BG455193 NF100G04PL1F1034 Phosphate starved leaf Medicago truncatula cDNA
clone NF100G04PL 5', mRNA sequence
Length = 654
Score = 130 bits (278), Expect(2) = 3e-048
Identities = 49/103 (47%), Positives = 68/103 (66%)
Frame = -1 / +2
Query: 768 PGEVEHFDMSGTAMGAMARPGMADKLRAGGVLRILYRRVPCKYTGVNIAFKVDQGANPYY 589
P + HFD+SG A G MA G +LR G +++LYRR C Y G NIAF+V++G+ P++
Sbjct: 191 PSDRTHFDLSGAAFGHMAVHGENGQLRNRGEIQVLYRRTTCVYAGKNIAFQVNEGSTPFW 370
Query: 588 FDVLIEFEDDDGDLSAVDLMEAGSGVWTPMAHNWGATWRLNNG 460
+L+EFED DGD+S++ + EAGS W M H WGA W + G
Sbjct: 371 LSLLVEFEDGDGDISSMHIQEAGSSEWLQMNHLWGANWNIVTG 499
Score = 84.5 bits (178), Expect(2) = 3e-048
Identities = 31/62 (50%), Positives = 41/62 (66%)
Frame = -1 / +2
Query: 963 GGACGYQTAVGKKPFDSMIAAGSTPLYRGGEGCGACYEVKCTTNAACSGQPVTIVITDQS 784
GGACGY + V KP + + A LY+ GEGCG CY+VKC + CS + VT++ITD+
Sbjct: 2 GGACGYGSLVDVKPLRARVGAVGPVLYKKGEGCGECYKVKCLDHTICSKRAVTVIITDEC 181
Query: 783 PG 778
PG
Sbjct: 182 PG 187
>gb|AL379968.1|AL379968 MtBB48E05F1 MtBB Medicago truncatula cDNA clone MtBB48E05 T3, mRNA
sequence
Length = 476
Score = 130 bits (278), Expect(2) = 1e-041
Identities = 49/103 (47%), Positives = 68/103 (66%)
Frame = -1 / +2
Query: 768 PGEVEHFDMSGTAMGAMARPGMADKLRAGGVLRILYRRVPCKYTGVNIAFKVDQGANPYY 589
P + HFD+SG A G MA G +LR G +++LYRR C Y G NIAF+V++G+ P++
Sbjct: 119 PSDRTHFDLSGAAFGHMAVHGENGQLRNRGEIQVLYRRTTCVYAGKNIAFQVNEGSTPFW 298
Query: 588 FDVLIEFEDDDGDLSAVDLMEAGSGVWTPMAHNWGATWRLNNG 460
+L+EFED DGD+S++ + EAGS W M H WGA W + G
Sbjct: 299 LSLLVEFEDGDGDISSMHIQEAGSSEWLQMNHLWGANWNIVTG 427
Score = 62.5 bits (130), Expect(2) = 1e-041
Identities = 21/37 (56%), Positives = 28/37 (75%)
Frame = -1 / +2
Query: 888 LYRGGEGCGACYEVKCTTNAACSGQPVTIVITDQSPG 778
LY+ GEGCG CY+VKC + CS + VT++ITD+ PG
Sbjct: 5 LYKKGEGCGECYKVKCLDHTICSKRAVTVIITDECPG 115
>gb|BG453428.1|BG453428 NF091G11LF1F1085 Developing leaf Medicago truncatula cDNA clone
NF091G11LF 5', mRNA sequence
Length = 638
Score = 96.4 bits (204), Expect(3) = 4e-036
Identities = 41/77 (53%), Positives = 53/77 (68%)
Frame = -1 / +3
Query: 753 HFDMSGTAMGAMARPGMADKLRAGGVLRILYRRVPCKYTGVNIAFKVDQGANPYYFDVLI 574
HFD+SG+A G+MA G ++LR G + I +RRV C Y G IAF VD G+N YF LI
Sbjct: 27 HFDLSGSAFGSMAVSGEDEELRNAGKIVIEHRRVQCNYGGSTIAFHVDSGSNQEYFAALI 206
Query: 573 EFEDDDGDLSAVDLMEA 523
E+E+ DGDL+ V+L EA
Sbjct: 207 EYEEGDGDLNKVELKEA 257
Score = 50.6 bits (104), Expect(3) = 4e-036
Identities = 18/30 (60%), Positives = 24/30 (80%)
Frame = -1 / +3
Query: 426 TSDSRRVLVANNAIPAAWKPGKTYRSLVNY 337
T S + +VA+N IPA WKPG+TYRS+VN+
Sbjct: 360 TLKSGKTIVAHNVIPAGWKPGQTYRSIVNF 449
Score = 45.1 bits (92), Expect(3) = 4e-036
Identities = 15/33 (45%), Positives = 20/33 (60%)
Frame = -1 / +3
Query: 519 SGVWTPMAHNWGATWRLNNGRKLKAPFGLRLTS 421
S W M +WGA W+ + G LKAP +RLT+
Sbjct: 264 SSSWDTMQQSWGAVWKFDKGAPLKAPISIRLTT 362
>gb|BG448066.1|BG448066 NF105C08EC1F1056 Elicited cell culture Medicago truncatula cDNA
clone NF105C08EC 5', mRNA sequence
Length = 652
Score = 97.7 bits (207), Expect(2) = 6e-036
Identities = 37/67 (55%), Positives = 46/67 (68%)
Frame = -1 / +1
Query: 978 GDGSDGGACGYQTAVGKKPFDSMIAAGSTPLYRGGEGCGACYEVKCTTNAACSGQPVTIV 799
GDGS GGACGY T V KP + + A L+ GEGCGACY+VKC N+ CS + VT++
Sbjct: 256 GDGSTGGACGYGTLVDVKPLRARVGAVGPVLFMKGEGCGACYKVKCLDNSICSRRAVTVI 435
Query: 798 ITDQSPG 778
ITD+ PG
Sbjct: 436 ITDECPG 456
Score = 75.8 bits (159), Expect(2) = 6e-036
Identities = 29/64 (45%), Positives = 41/64 (64%)
Frame = -1 / +1
Query: 768 PGEVEHFDMSGTAMGAMARPGMADKLRAGGVLRILYRRVPCKYTGVNIAFKVDQGANPYY 589
P + HFD+SG A G MA G +LR G + ++YRR PCKY G IAF V++G+ P++
Sbjct: 460 PTDTTHFDLSGAAFGHMAISGENGQLRNRGQIPVIYRRTPCKYPGKKIAFHVNEGSTPFW 639
Query: 588 FDVL 577
+L
Sbjct: 640 LSLL 651
>gb|BG452295.1|BG452295 NF085E05LF1F1039 Developing leaf Medicago truncatula cDNA clone
NF085E05LF 5', mRNA sequence
Length = 578
Score = 93.6 bits (198), Expect(2) = 8e-033
Identities = 35/67 (52%), Positives = 45/67 (67%)
Frame = -1 / +3
Query: 978 GDGSDGGACGYQTAVGKKPFDSMIAAGSTPLYRGGEGCGACYEVKCTTNAACSGQPVTIV 799
GDGS GGACGY + V KP + + A LY+ GEGCG CY+VKC + CS + VT++
Sbjct: 159 GDGSRGGACGYGSLVDVKPLRARVGAVGPVLYKKGEGCGECYKVKCLDHTICSKRAVTVI 338
Query: 798 ITDQSPG 778
ITD+ PG
Sbjct: 339 ITDECPG 359
Score = 69.3 bits (145), Expect(2) = 8e-033
Identities = 27/60 (45%), Positives = 39/60 (65%)
Frame = -1 / +3
Query: 768 PGEVEHFDMSGTAMGAMARPGMADKLRAGGVLRILYRRVPCKYTGVNIAFKVDQGANPYY 589
P + HFD+SG A G MA G +L G +++LYRR C Y G NIAF+V++G+ P++
Sbjct: 363 PSDRTHFDLSGAAFGHMAVHGENGQLXNRGEIQVLYRRTTCVYAGKNIAFQVNEGSTPFW 542
>gb|AC148226.7| Medicago truncatula clone mth2-5i20, complete sequence
Length = 84259
Score = 59.7 bits (124), Expect(5) = 1e-031
Identities = 21/39 (53%), Positives = 26/39 (66%)
Frame = -1 / +2
Query: 963 GGACGYQTAVGKKPFDSMIAAGSTPLYRGGEGCGACYEV 847
GGACGY +V P MI+AG ++ GG GCGACY+V
Sbjct: 63992 GGACGYVESVENPPLSKMISAGGPSIFLGGRGCGACYQV 64108
Score = 48.7 bits (100), Expect(5) = 1e-031
Identities = 18/54 (33%), Positives = 28/54 (51%)
Frame = -1 / +1
Query: 573 EFEDDDGDLSAVDLMEAGSGVWTPMAHNWGATWRLNNGRKLKAPFGLRLTSDSR 412
E E ++G+ V + + S W PM +WG+ W N G L+ PF ++L R
Sbjct: 65905 EIEYENGNGDLVQVELSQSNTWHPMFRSWGSRWAFNTGSPLQTPFSIKLVETLR 66066
Score = 48.3 bits (99), Expect(5) = 1e-031
Identities = 19/36 (52%), Positives = 23/36 (63%)
Frame = -1 / +1
Query: 762 EVEHFDMSGTAMGAMARPGMADKLRAGGVLRILYRR 655
E HFD+SGTA G++A P AD LR G + I Y R
Sbjct: 65602 ESAHFDLSGTAFGSLANPNQADNLRNAGKINIRYTR 65709
Score = 46.9 bits (96), Expect(5) = 1e-031
Identities = 16/25 (64%), Positives = 20/25 (80%)
Frame = -1 / +1
Query: 855 YEVKCTTNAACSGQPVTIVITDQSP 781
++VKCT N+ACSG PV +VITD P
Sbjct: 65521 FQVKCTENSACSGSPVNVVITDNCP 65595
Score = 42.3 bits (86), Expect(5) = 1e-031
Identities = 14/27 (51%), Positives = 19/27 (70%)
Frame = -1 / +3
Query: 417 SRRVLVANNAIPAAWKPGKTYRSLVNY 337
S ++A N IP W+PG+ YRSLVN+
Sbjct: 70659 SNNTIIAENVIPQNWQPGQVYRSLVNF 70739
>gb|AC174309.3| Medicago truncatula clone mth2-139k12, WORKING DRAFT SEQUENCE, 2
unordered pieces
Length = 111023
Score = 59.7 bits (124), Expect(5) = 3e-031
Identities = 21/39 (53%), Positives = 26/39 (66%)
Frame = -1 / +3
Query: 963 GGACGYQTAVGKKPFDSMIAAGSTPLYRGGEGCGACYEV 847
GGACGY +V P MI+AG ++ GG GCGACY+V
Sbjct: 90765 GGACGYVESVENPPLSKMISAGGPSIFLGGRGCGACYQV 90881
Score = 48.7 bits (100), Expect(5) = 3e-031
Identities = 18/54 (33%), Positives = 28/54 (51%)
Frame = -1 / +2
Query: 573 EFEDDDGDLSAVDLMEAGSGVWTPMAHNWGATWRLNNGRKLKAPFGLRLTSDSR 412
E E ++G+ V + + S W PM +WG+ W N G L+ PF ++L R
Sbjct: 92678 EIEYENGNGDLVQVELSQSNTWHPMFRSWGSRWAFNTGSPLQTPFSIKLVETLR 92839
Score = 48.3 bits (99), Expect(5) = 3e-031
Identities = 19/36 (52%), Positives = 23/36 (63%)
Frame = -1 / +2
Query: 762 EVEHFDMSGTAMGAMARPGMADKLRAGGVLRILYRR 655
E HFD+SGTA G++A P AD LR G + I Y R
Sbjct: 92375 ESAHFDLSGTAFGSLANPNQADNLRNAGKINIRYTR 92482
Score = 46.9 bits (96), Expect(5) = 3e-031
Identities = 16/25 (64%), Positives = 20/25 (80%)
Frame = -1 / +2
Query: 855 YEVKCTTNAACSGQPVTIVITDQSP 781
++VKCT N+ACSG PV +VITD P
Sbjct: 92294 FQVKCTENSACSGSPVNVVITDNCP 92368
Score = 42.3 bits (86), Expect(5) = 3e-031
Identities = 14/27 (51%), Positives = 19/27 (70%)
Frame = -1 / +1
Query: 417 SRRVLVANNAIPAAWKPGKTYRSLVNY 337
S ++A N IP W+PG+ YRSLVN+
Sbjct: 97432 SNNTIIAENVIPQNWQPGQVYRSLVNF 97512
>gb|AL379969.1|AL379969 MtBB48E05R1 MtBB Medicago truncatula cDNA clone MtBB48E05 T7, mRNA
sequence
Length = 488
Score = 104 bits (222), Expect(2) = 7e-028
Identities = 36/74 (48%), Positives = 52/74 (70%)
Frame = -1 / +2
Query: 681 GVLRILYRRVPCKYTGVNIAFKVDQGANPYYFDVLIEFEDDDGDLSAVDLMEAGSGVWTP 502
G +++LYRR C Y G NIAF+V++G+ P++ +L+EFED DGD+S++ + EAGS W
Sbjct: 2 GEIQVLYRRTTCVYAGKNIAFQVNEGSTPFWLSLLVEFEDGDGDISSMHIQEAGSSEWLQ 181
Query: 501 MAHNWGATWRLNNG 460
M H WGA W + G
Sbjct: 182 MNHLWGANWNIVTG 223
Score = 41.8 bits (85), Expect(2) = 7e-028
Identities = 14/39 (35%), Positives = 25/39 (64%)
Frame = -1 / +2
Query: 453 LKAPFGLRLTSDSRRVLVANNAIPAAWKPGKTYRSLVNY 337
L+ PF ++L++ + + L A + IP+ W P TY S +N+
Sbjct: 227 LRGPFSVKLSTSTGKSLTAKDVIPSHWSPKSTYTSRLNF 343
>gb|AW687498.2|AW687498 NF010C04RT1F1033 Developing root Medicago truncatula cDNA clone
NF010C04RT 5', mRNA sequence
Length = 522
Score = 105 bits (225), Expect(2) = 2e-027
Identities = 38/79 (48%), Positives = 54/79 (68%)
Frame = -1 / +2
Query: 696 KLRAGGVLRILYRRVPCKYTGVNIAFKVDQGANPYYFDVLIEFEDDDGDLSAVDLMEAGS 517
+LR G + +LYRR C Y G N+AF+V++G+ PY+ +L+EFED DGD+S++ + EAGS
Sbjct: 116 QLRNRGEIPVLYRRTTCVYAGKNLAFQVNEGSTPYWLSLLVEFEDGDGDISSMYIQEAGS 295
Query: 516 GVWTPMAHNWGATWRLNNG 460
W M H WGA W + G
Sbjct: 296 SEWLQMNHLWGANWNIVTG 352
Score = 39.1 bits (79), Expect(2) = 2e-027
Identities = 12/39 (30%), Positives = 24/39 (61%)
Frame = -1 / +2
Query: 453 LKAPFGLRLTSDSRRVLVANNAIPAAWKPGKTYRSLVNY 337
L+ PF ++L++ + + + + IP+ W P TY S +N+
Sbjct: 356 LRGPFSVKLSTSTGKSITVKDVIPSNWSPKSTYTSRLNF 472
>gb|BF648216.1|BF648216 NF045D01EC1F1012 Elicited cell culture Medicago truncatula cDNA
clone NF045D01EC 5', mRNA sequence
Length = 596
Score = 98.2 bits (208), Expect(2) = 1e-026
Identities = 35/78 (44%), Positives = 50/78 (64%)
Frame = -1 / +3
Query: 693 LRAGGVLRILYRRVPCKYTGVNIAFKVDQGANPYYFDVLIEFEDDDGDLSAVDLMEAGSG 514
LR G + ++YRR PCKY G IAF V++G+ P++ +L+EFED +GD+ + + E GS
Sbjct: 54 LRNRGQIPVIYRRTPCKYPGKKIAFHVNEGSTPFWLSLLVEFEDAEGDIGTMHIRENGSS 233
Query: 513 VWTPMAHNWGATWRLNNG 460
W M H WGA W + G
Sbjct: 234 EWLQMNHLWGANWCIIGG 287
Score = 44.1 bits (90), Expect(2) = 1e-026
Identities = 16/42 (38%), Positives = 26/42 (61%)
Frame = -1 / +3
Query: 462 GRKLKAPFGLRLTSDSRRVLVANNAIPAAWKPGKTYRSLVNY 337
G L+ PF ++L+S + R L A + +P+ W P TY S +N+
Sbjct: 282 GGPLRGPFSVKLSSSTGRTLSARDVVPSNWVPKATYTSRLNF 407
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 972,183,350
Number of Sequences: 392609
Number of extensions: 10458313
Number of successful extensions: 45593
Number of sequences better than 1.0e-020: 17
length of database: 147,244,331
effective HSP length: 56
effective length of database: 125,258,227
effective search space used: 49476999665
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)