TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 129373.2.1
(1297 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DW017752.1|DW017752 EST1226713 MTY Medicago truncatula c... 87 3e-026
gb|CX534598.1|CX534598 s13dNF74D09MJ078_330898 Methyl Jasmo... 87 2e-024
gb|BF646792.1|BF646792 NF072C01EC1F1004 Elicited cell cultu... 87 2e-024
gb|DW017309.1|DW017309 EST1226270 MTY Medicago truncatula c... 82 1e-023
gb|CX533956.1|CX533956 s13dNF0NC02MJ006_321676 Methyl Jasmo... 87 4e-023
gb|BI272934.1|BI272934 NF091G11FL1F1087 Developing flower M... 83 1e-022
gb|BG644339.1|BG644339 EST505958 KV3 Medicago truncatula cD... 66 3e-022
>gb|DW017752.1|DW017752 EST1226713 MTY Medicago truncatula cDNA clone MTYAX65, mRNA sequence
Length = 711
Score = 86.8 bits (183), Expect(3) = 3e-026
Identities = 35/63 (55%), Positives = 46/63 (73%)
Frame = -1 / +2
Query: 1093 LGESKKSDSALMYVKVDDKKLAIGTLSIDKYPQIQFDLVFNKEFELSHTSKTTSVFFSGY 914
+ + K SD +YVK D +KL +GTLS +K+PQ FDLVF K+FELSH K SVFFSG+
Sbjct: 170 VSKDKGSDPVSLYVKFDGQKLQLGTLSSEKFPQTSFDLVFGKKFELSHNWKYGSVFFSGF 349
Query: 913 KVE 905
++E
Sbjct: 350 RIE 358
Score = 48.3 bits (99), Expect(3) = 3e-026
Identities = 18/36 (50%), Positives = 25/36 (69%)
Frame = -1 / +2
Query: 1183 MEFWGLEVKPGSTVKCEPGHGFILHVSQAALGESKK 1076
MEFWG+EVK G ++K P + ++H+S A LGE K
Sbjct: 71 MEFWGVEVKLGESLKVVPENSELIHISSACLGEVSK 178
Score = 28.6 bits (56), Expect(3) = 3e-026
Identities = 12/22 (54%), Positives = 16/22 (72%)
Frame = -1 / +2
Query: 544 KRGAENALKTPLSDKKAKVATP 479
KR AE++ +TP+ KKAK TP
Sbjct: 485 KRVAESSKETPVPVKKAKFVTP 550
>gb|CX534598.1|CX534598 s13dNF74D09MJ078_330898 Methyl Jasmonate-Elicited Root Cell
Suspension Culture Medicago truncatula cDNA, mRNA
sequence
Length = 603
Score = 86.8 bits (183), Expect(2) = 2e-024
Identities = 35/63 (55%), Positives = 46/63 (73%)
Frame = -1 / +3
Query: 1093 LGESKKSDSALMYVKVDDKKLAIGTLSIDKYPQIQFDLVFNKEFELSHTSKTTSVFFSGY 914
+ + K SD +YVK D +KL +GTLS +K+PQ FDLVF K+FELSH K SVFFSG+
Sbjct: 123 VSKDKGSDPVSLYVKFDGQKLQLGTLSSEKFPQTSFDLVFGKKFELSHNWKYGSVFFSGF 302
Query: 913 KVE 905
++E
Sbjct: 303 RIE 311
Score = 48.3 bits (99), Expect(2) = 2e-024
Identities = 18/36 (50%), Positives = 25/36 (69%)
Frame = -1 / +3
Query: 1183 MEFWGLEVKPGSTVKCEPGHGFILHVSQAALGESKK 1076
MEFWG+EVK G ++K P + ++H+S A LGE K
Sbjct: 24 MEFWGVEVKLGESLKVVPENSELIHISSACLGEVSK 131
>gb|BF646792.1|BF646792 NF072C01EC1F1004 Elicited cell culture Medicago truncatula cDNA clone
NF072C01EC 5', mRNA sequence
Length = 565
Score = 86.8 bits (183), Expect(2) = 2e-024
Identities = 35/63 (55%), Positives = 46/63 (73%)
Frame = -1 / +2
Query: 1093 LGESKKSDSALMYVKVDDKKLAIGTLSIDKYPQIQFDLVFNKEFELSHTSKTTSVFFSGY 914
+ + K SD +YVK D +KL +GTLS +K+PQ FDLVF K+FELSH K SVFFSG+
Sbjct: 140 VSKDKGSDPVSLYVKFDGQKLQLGTLSSEKFPQTSFDLVFGKKFELSHNWKYGSVFFSGF 319
Query: 913 KVE 905
++E
Sbjct: 320 RIE 328
Score = 48.3 bits (99), Expect(2) = 2e-024
Identities = 18/36 (50%), Positives = 25/36 (69%)
Frame = -1 / +2
Query: 1183 MEFWGLEVKPGSTVKCEPGHGFILHVSQAALGESKK 1076
MEFWG+EVK G ++K P + ++H+S A LGE K
Sbjct: 41 MEFWGVEVKLGESLKVVPENSELIHISSACLGEVSK 148
>gb|DW017309.1|DW017309 EST1226270 MTY Medicago truncatula cDNA clone MTYAS06, mRNA sequence
Length = 845
Score = 81.7 bits (172), Expect(3) = 1e-023
Identities = 34/69 (49%), Positives = 46/69 (66%)
Frame = -1 / +2
Query: 1117 ILHVSQAALGESKKSDSALMYVKVDDKKLAIGTLSIDKYPQIQFDLVFNKEFELSHTSKT 938
I V+ + K ++S ++Y+KV ++KL +GTL+ DK PQI D+V KEFELSH SK
Sbjct: 161 ISQVALGEAKKDKPNESVVIYLKVGEQKLVLGTLTKDKIPQISLDIVLEKEFELSHNSKA 340
Query: 937 TSVFFSGYK 911
SV F GYK
Sbjct: 341 ASVHFCGYK 367
Score = 38.2 bits (77), Expect(3) = 1e-023
Identities = 16/24 (66%), Positives = 19/24 (79%)
Frame = -1 / +2
Query: 550 GKKRGAENALKTPLSDKKAKVATP 479
GKKR E+A KTP+S KK+K ATP
Sbjct: 728 GKKRPNESASKTPISSKKSKNATP 799
Score = 35.4 bits (71), Expect(3) = 1e-023
Identities = 12/18 (66%), Positives = 14/18 (77%)
Frame = -1 / +2
Query: 1183 MEFWGLEVKPGSTVKCEP 1130
MEFWG EVK G T+K +P
Sbjct: 80 MEFWGAEVKVGETIKIDP 133
>gb|CX533956.1|CX533956 s13dNF0NC02MJ006_321676 Methyl Jasmonate-Elicited Root Cell
Suspension Culture Medicago truncatula cDNA, mRNA
sequence
Length = 568
Score = 86.8 bits (183), Expect(2) = 4e-023
Identities = 35/63 (55%), Positives = 46/63 (73%)
Frame = -1 / +1
Query: 1093 LGESKKSDSALMYVKVDDKKLAIGTLSIDKYPQIQFDLVFNKEFELSHTSKTTSVFFSGY 914
+ + K SD +YVK D +KL +GTLS +K+PQ FDLVF K+FELSH K SVFFSG+
Sbjct: 103 VSKDKGSDPVSLYVKFDGQKLQLGTLSSEKFPQTSFDLVFGKKFELSHNWKYGSVFFSGF 282
Query: 913 KVE 905
++E
Sbjct: 283 RIE 291
Score = 43.7 bits (89), Expect(2) = 4e-023
Identities = 16/34 (47%), Positives = 23/34 (67%)
Frame = -1 / +1
Query: 1177 FWGLEVKPGSTVKCEPGHGFILHVSQAALGESKK 1076
FWG+EVK G ++K P + ++H+S A LGE K
Sbjct: 10 FWGVEVKLGESLKVVPENSELIHISSACLGEVSK 111
>gb|BI272934.1|BI272934 NF091G11FL1F1087 Developing flower Medicago truncatula cDNA clone
NF091G11FL 5', mRNA sequence
Length = 474
Score = 82.6 bits (174), Expect(2) = 1e-022
Identities = 34/63 (53%), Positives = 44/63 (69%)
Frame = -1 / +1
Query: 1093 LGESKKSDSALMYVKVDDKKLAIGTLSIDKYPQIQFDLVFNKEFELSHTSKTTSVFFSGY 914
+ + K SD +YVK D +KL +GTLS +K+P FDLVF K+FELSH K SVFFSG+
Sbjct: 133 VSKDKGSDPVSLYVKFDGQKLQLGTLSSEKFPXTSFDLVFGKKFELSHNWKYGSVFFSGF 312
Query: 913 KVE 905
+E
Sbjct: 313 XIE 321
Score = 46.0 bits (94), Expect(2) = 1e-022
Identities = 17/36 (47%), Positives = 24/36 (66%)
Frame = -1 / +1
Query: 1183 MEFWGLEVKPGSTVKCEPGHGFILHVSQAALGESKK 1076
MEFWG+EVK G ++K P + ++H+S A GE K
Sbjct: 34 MEFWGVEVKLGESLKVVPENSELIHISSACXGEVSK 141
>gb|BG644339.1|BG644339 EST505958 KV3 Medicago truncatula cDNA clone pKV3-37A15 5' end, mRNA
sequence
Length = 763
Score = 65.7 bits (137), Expect(3) = 3e-022
Identities = 29/61 (47%), Positives = 36/61 (59%)
Frame = -1 / +1
Query: 1081 KKSDSALMYVKVDDKKLAIGTLSIDKYPQIQFDLVFNKEFELSHTSKTTSVFFSGYKVEQ 902
K++ + + V D IGTLS + PQ+ FDLVF KEFELSH K SV F GY +
Sbjct: 133 KEAKYVPLRLTVGDNSYVIGTLSAEDRPQLMFDLVFEKEFELSHGWKNGSVHFIGYTADD 312
Query: 901 P 899
P
Sbjct: 313 P 315
Score = 54.7 bits (113), Expect(3) = 3e-022
Identities = 22/36 (61%), Positives = 28/36 (77%)
Frame = -1 / +1
Query: 1186 SMEFWGLEVKPGSTVKCEPGHGFILHVSQAALGESK 1079
SMEFWG+EVK G T+K +P ++HVSQAA+GE K
Sbjct: 19 SMEFWGVEVKAGQTLKVKPELFKLIHVSQAAIGEVK 126
Score = 29.5 bits (58), Expect(3) = 3e-022
Identities = 17/38 (44%), Positives = 19/38 (50%)
Frame = -1 / +1
Query: 547 KKRGAENALKTPLSDKKAKVATPPAQKTGGKKGATHVA 434
KKR A + P S KKAK TP + G K AT A
Sbjct: 646 KKRPAPSPKVAPGSGKKAKQVTPAHKSGGKKGPATPFA 759
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 1,172,152,954
Number of Sequences: 392609
Number of extensions: 17054681
Number of successful extensions: 72200
Number of sequences better than 1.0e-020: 10
length of database: 147,244,331
effective HSP length: 56
effective length of database: 125,258,227
effective search space used: 46971835125
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)