TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCN21e10.yg.2.1
(247 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO516533.1|CO516533 s13dSG57C0100002_446394 Glandular tr... 22 0.26
gb|CO512300.1|CO512300 s13dSG68D0100010_114114 Glandular tr... 28 0.29
gb|AW698895.1|AW698895 r3 non-glandular-haired subtracted c... 26 1.0
gb|AJ410153.1|AJ410153 AJ410153 Medicago sativa library (Ka... 26 1.0
gb|DR159666.1|DR159666 EST16 Medicago sativa cDNA-AFLP Medi... 23 1.7
gb|CB165091.2|CB165091 15III Alfalfa developing flower bud ... 25 1.9
gb|CO514754.1|CO514754 s13dSG55G0600052_328168 Glandular tr... 25 1.9
gb|CO515349.1|CO515349 s13dSG49A0700053_417757 Glandular tr... 25 1.9
gb|AF439380.1| Medicago sativa retrotransposon polyprotein ... 25 1.9
gb|CO516264.1|CO516264 s13dSG69D0300026_445856 Glandular tr... 25 2.6
gb|AW698350.1|AW698350 g29 glandular-haired subtracted cDNA... 20 4.4
gb|AW698680.1|AW698680 R116 non-glandular-haired subtracted... 22 4.7
emb|Z50800.1|MSASET2MR M.sativa mRNA for ASET2 24 5.0
emb|AJ966573.1| Medicago sativa subsp. x varia mRNA for put... 24 5.0
gb|AW698698.1|AW698698 R146 non-glandular-haired subtracted... 24 5.0
gb|CO513452.1|CO513452 s13dSG10D0300026_130068 Glandular tr... 24 5.0
emb|AJ619853.1| Medicago sativa mRNA for small GTPase Rac4 ... 24 5.0
gb|DQ122835.1| Medicago sativa clone QF3 zinc finger Glo3-l... 24 5.0
gb|AJ248336.1|AJ248336 AJ248336 Medicago sativa subsp. x va... 24 6.8
gb|CO512341.1|CO512341 s13dSG68G1100084_114196 Glandular tr... 24 6.8
gb|AY851389.1| Medicago sativa leaf sucrose-phosphate synth... 24 6.8
gb|AI083093.1|AI083093 MSB4 Medicago sativa shoot tip Lambd... 23 9.4
gb|CO512139.1|CO512139 s13dSG34D0300026_108598 Glandular tr... 23 9.4
gb|CO513326.1|CO513326 s13dSG37F1200095_129816 Glandular tr... 23 9.4
gb|CO514466.1|CO514466 s13dSG43A0800055_327592 Glandular tr... 23 9.4
gb|CO516213.1|CO516213 s13dSG67G0800068_445754 Glandular tr... 23 9.4
gb|CO516827.1|CO516827 s13dSG98C0200006_446982 Glandular tr... 23 9.4
gb|U41472.1|MSU41472 Medicago sativa pectate lyase homolog ... 23 9.4
emb|X13287.1|MSNOD25 Medicago sativa nodulin-25 gene 23 9.4
emb|X70707.1|MSCDC2 M.sativa mRNA for CDC2 kinase 23 9.4
gb|AF498987.1| Medicago sativa nodule-specific glycine-rich... 22 9.7
>gb|CO516533.1|CO516533 s13dSG57C0100002_446394 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 296
Score = 21.7 bits (41), Expect(2) = 0.26
Identities = 6/6 (100%), Positives = 6/6 (100%)
Frame = -1 / +1
Query: 133 SKWQWR 116
SKWQWR
Sbjct: 97 SKWQWR 114
Score = 21.2 bits (40), Expect(2) = 0.26
Identities = 6/19 (31%), Positives = 11/19 (57%)
Frame = -2 / +2
Query: 75 FDFFRMLSCYYTTIGFYFS 19
F + ++ CY + FYF+
Sbjct: 197 FGYAFLVFCYMNNMSFYFN 253
>gb|CO512300.1|CO512300 s13dSG68D0100010_114114 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 571
Score = 28.1 bits (55), Expect = 0.29
Identities = 9/16 (56%), Positives = 12/16 (75%)
Frame = -2 / -2
Query: 96 IYRLGHRFDFFRMLSC 49
++R HRFD FR L+C
Sbjct: 294 VFRYNHRFDTFRDLNC 247
>gb|AW698895.1|AW698895 r3 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 370
Score = 26.3 bits (51), Expect = 1.0
Identities = 12/23 (52%), Positives = 15/23 (65%)
Frame = -1 / -3
Query: 82 SSL*FLQDAVLLLHNNWFLLQYL 14
+SL L ++LL NNW LLQ L
Sbjct: 80 TSLGLLPKNIVLLRNNWKLLQPL 12
>gb|AJ410153.1|AJ410153 AJ410153 Medicago sativa library (Kalo P) Medicago sativa cDNA
clone U742, mRNA sequence
Length = 722
Score = 26.3 bits (51), Expect = 1.0
Identities = 11/35 (31%), Positives = 19/35 (54%)
Frame = +1 / -2
Query: 88 PVDVTAQRLFAIAICYLCLKQRYLIETYIPSFANL 192
P + A R + CLK +L+ T++ SFA++
Sbjct: 373 PRKIIAVRRLSCMTIVHCLKTTHLVPTFV*SFADI 269
>gb|DR159666.1|DR159666 EST16 Medicago sativa cDNA-AFLP Medicago sativa cDNA clone M24 5',
mRNA sequence
Length = 477
Score = 22.6 bits (43), Expect(2) = 1.7
Identities = 9/25 (36%), Positives = 15/25 (60%)
Frame = +2 / +1
Query: 131 AIFASNRDI*LRPTSLPLPTCMYSW 205
A+ +SN L+P+ +PL + SW
Sbjct: 187 ALTSSNISTHLQPSLMPLAFTLMSW 261
Score = 18.0 bits (33), Expect(2) = 1.7
Identities = 6/13 (46%), Positives = 7/13 (53%)
Frame = +3 / +2
Query: 96 CHGSAFVRHCHLL 134
C G F HC L+
Sbjct: 101 CFGEVFRCHCCLV 139
>gb|CB165091.2|CB165091 15III Alfalfa developing flower bud library Medicago sativa cDNA,
mRNA sequence
Length = 289
Score = 25.4 bits (49), Expect = 1.9
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = +2 / +3
Query: 122 LPFAIFASNRDI*LRPTSLP 181
LPF+ F +N D *LR + LP
Sbjct: 21 LPFSQFQTNADT*LRNSILP 80
>gb|CO514754.1|CO514754 s13dSG55G0600052_328168 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 415
Score = 25.4 bits (49), Expect = 1.9
Identities = 10/23 (43%), Positives = 12/23 (52%)
Frame = -2 / +1
Query: 69 FFRMLSCYYTTIGFYFSTCPGGR 1
FF LS YY F+F + G R
Sbjct: 28 FFSSLSFYYCVFSFFFLSKNGAR 96
>gb|CO515349.1|CO515349 s13dSG49A0700053_417757 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 543
Score = 25.4 bits (49), Expect = 1.9
Identities = 10/33 (30%), Positives = 16/33 (48%)
Frame = -2 / -2
Query: 102 RDIYRLGHRFDFFRMLSCYYTTIGFYFSTCPGG 4
+D+Y G R D L + T + + +C GG
Sbjct: 293 KDLYMHGFRLDTTVSLLLFLTGVAVFRRSCDGG 195
>gb|AF439380.1| Medicago sativa retrotransposon polyprotein gene interrupted by LTR
retroelement MCIRE, complete sequence
Length = 18789
Score = 25.4 bits (49), Expect = 1.9
Identities = 8/11 (72%), Positives = 10/11 (90%)
Frame = +1 / -2
Query: 175 PSFANLHVFMV 207
P+FANLH FM+
Sbjct: 8309 PAFANLHFFMI 8277
Score = 25.4 bits (49), Expect = 1.9
Identities = 8/11 (72%), Positives = 10/11 (90%)
Frame = +1 / -1
Query: 175 PSFANLHVFMV 207
P+FANLH FM+
Sbjct: 12369 PAFANLHFFMI 12337
Score = 24.4 bits (47), Expect = 3.6
Identities = 12/38 (31%), Positives = 19/38 (50%)
Frame = +1 / +2
Query: 85 *PVDVTAQRLFAIAICYLCLKQRYLIETYIPSFANLHV 198
*P T+ F ++C L + + YL+ TY+ HV
Sbjct: 3977 *PAIKTSSF*FLSSLCSLYIGRDYLLSTYVHCIYIYHV 4090
Score = 23.1 bits (44), Expect = 9.4
Identities = 7/14 (50%), Positives = 11/14 (78%)
Frame = +1 / -3
Query: 181 FANLHVFMVSNIAF 222
F + H+F++SNI F
Sbjct: 3382 FTSYHIFILSNIPF 3341
>gb|CO516264.1|CO516264 s13dSG69D0300026_445856 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 497
Score = 24.9 bits (48), Expect = 2.6
Identities = 9/17 (52%), Positives = 10/17 (58%)
Frame = -2 / +3
Query: 69 FFRMLSCYYTTIGFYFS 19
FF LS YY F+FS
Sbjct: 30 FFSSLSFYYCVFSFFFS 80
>gb|AW698350.1|AW698350 g29 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 274
Score = 20.3 bits (38), Expect(2) = 4.4
Identities = 6/6 (100%), Positives = 6/6 (100%)
Frame = -2 / +1
Query: 18 TCPGGR 1
TCPGGR
Sbjct: 253 TCPGGR 270
Score = 18.0 bits (33), Expect(2) = 4.4
Identities = 10/27 (37%), Positives = 13/27 (48%)
Frame = -3 / +2
Query: 83 VIALISSGCCLVTTQQLVSTSVPARAA 3
VI ++ G L + VPARAA
Sbjct: 188 VIPVLRLGLPLCALRSSSGLLVPARAA 268
>gb|AW698680.1|AW698680 R116 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 290
Score = 22.1 bits (42), Expect(2) = 4.7
Identities = 7/7 (100%), Positives = 7/7 (100%)
Frame = -2 / +3
Query: 21 STCPGGR 1
STCPGGR
Sbjct: 267 STCPGGR 287
Score = 16.2 bits (29), Expect(2) = 4.7
Identities = 4/9 (44%), Positives = 6/9 (66%)
Frame = -1 / +1
Query: 190 SWQRKGCRS 164
+W R C+S
Sbjct: 1 AWSRPRCKS 27
>emb|Z50800.1|MSASET2MR M.sativa mRNA for ASET2
Length = 1193
Score = 24.0 bits (46), Expect = 5.0
Identities = 8/19 (42%), Positives = 12/19 (63%)
Frame = -2 / +2
Query: 63 RMLSCYYTTIGFYFSTCPG 7
R +C+ + YF+TCPG
Sbjct: 1064 RRETCHSSLRNRYFTTCPG 1120
>emb|AJ966573.1| Medicago sativa subsp. x varia mRNA for putative Rho GTPase (rop6
gene)
Length = 1266
Score = 24.0 bits (46), Expect = 5.0
Identities = 7/17 (41%), Positives = 12/17 (70%)
Frame = +3 / -1
Query: 135 SLPQTEIFD*DLHPFLC 185
S+PQ EI+ +H ++C
Sbjct: 300 SIPQPEIYSAPIHNYIC 250
>gb|AW698698.1|AW698698 R146 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 326
Score = 24.0 bits (46), Expect = 5.0
Identities = 10/26 (38%), Positives = 13/26 (50%)
Frame = +3 / +3
Query: 3 GRPGRY*SRNQLLCSNKTAS*RNQSD 80
GRPGRY ++ C N +SD
Sbjct: 6 GRPGRYXGDPKVYCGNTKGDKWKKSD 83
>gb|CO513452.1|CO513452 s13dSG10D0300026_130068 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 599
Score = 24.0 bits (46), Expect = 5.0
Identities = 12/42 (28%), Positives = 22/42 (52%)
Frame = +1 / +1
Query: 91 VDVTAQRLFAIAICYLCLKQRYLIETYIPSFANLHVFMVSNI 216
+D+T+ R + CY Y+IE + F++ + +VS I
Sbjct: 52 MDLTS*RYLCMNQCY*YHNMNYMIECFFVFFSHKWL*LVSAI 177
>emb|AJ619853.1| Medicago sativa mRNA for small GTPase Rac4 (rac4 gene)
Length = 594
Score = 24.0 bits (46), Expect = 5.0
Identities = 7/17 (41%), Positives = 12/17 (70%)
Frame = +3 / -1
Query: 135 SLPQTEIFD*DLHPFLC 185
S+PQ EI+ +H ++C
Sbjct: 183 SIPQPEIYSAPIHNYIC 133
>gb|DQ122835.1| Medicago sativa clone QF3 zinc finger Glo3-like protein mRNA,
partial cds
Length = 658
Score = 24.0 bits (46), Expect = 5.0
Identities = 8/16 (50%), Positives = 9/16 (56%)
Frame = -2 / +3
Query: 51 CYYTTIGFYFSTCPGG 4
C T +FS CPGG
Sbjct: 606 CLVCTTCCFFSVCPGG 653
>gb|AJ248336.1|AJ248336 AJ248336 Medicago sativa subsp. x varia young root nodule Medicago
sativa subsp. x varia cDNA clone MsNod658, mRNA sequence
Length = 1004
Score = 23.5 bits (45), Expect = 6.8
Identities = 11/27 (40%), Positives = 14/27 (51%)
Frame = +1 / -2
Query: 97 VTAQRLFAIAICYLCLKQRYLIETYIP 177
V L IC++ R LIET+IP
Sbjct: 118 VPLHSLQGCCICWIIDGPRCLIETFIP 38
>gb|CO512341.1|CO512341 s13dSG68G1100084_114196 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 546
Score = 23.5 bits (45), Expect = 6.8
Identities = 9/22 (40%), Positives = 10/22 (45%)
Frame = -2 / +3
Query: 69 FFRMLSCYYTTIGFYFSTCPGG 4
FF LS YY F+F G
Sbjct: 30 FFSSLSFYYCVFSFFFFLSKNG 95
>gb|AY851389.1| Medicago sativa leaf sucrose-phosphate synthase mRNA, partial cds
Length = 645
Score = 23.5 bits (45), Expect = 6.8
Identities = 11/32 (34%), Positives = 16/32 (50%)
Frame = -2 / -1
Query: 138 KIANGNGEQTLSRDIYRLGHRFDFFRMLSCYY 43
+I ++ LSRDIYR+ R M C +
Sbjct: 303 QILECEAKEVLSRDIYRVDRRVFL*YMHPCSF 208
>gb|AI083093.1|AI083093 MSB4 Medicago sativa shoot tip Lambda ZAP cDNA library Medicago
sativa cDNA clone MSB4 similar to mudrA maize
transposon, mRNA sequence
Length = 733
Score = 23.1 bits (44), Expect = 9.4
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = -1 / +1
Query: 97 HLPARSSL*FLQDAVLLLHNNWFLLQ 20
HL + *FL + + + +NNW L+Q
Sbjct: 475 HLHDNNVS*FLTNILHVYNNNWGLVQ 552
>gb|CO512139.1|CO512139 s13dSG34D0300026_108598 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 543
Score = 23.1 bits (44), Expect = 9.4
Identities = 7/19 (36%), Positives = 12/19 (63%)
Frame = -2 / -2
Query: 63 RMLSCYYTTIGFYFSTCPG 7
R++S Y+T+ Y+ C G
Sbjct: 536 RIISPYFTSYFLYYGLCKG 480
>gb|CO513326.1|CO513326 s13dSG37F1200095_129816 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 546
Score = 23.1 bits (44), Expect = 9.4
Identities = 10/33 (30%), Positives = 17/33 (51%)
Frame = +3 / -3
Query: 42 CSNKTAS*RNQSDDLAGRCHGSAFVRHCHLLSL 140
CS S ++ +DLA + + ++ CH SL
Sbjct: 151 CST*DCSICHRCNDLAHHQNNTCYLHACHCFSL 53
>gb|CO514466.1|CO514466 s13dSG43A0800055_327592 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 577
Score = 23.1 bits (44), Expect = 9.4
Identities = 7/13 (53%), Positives = 9/13 (69%)
Frame = +2 / +1
Query: 167 PTSLPLPTCMYSW 205
P+S +PTC SW
Sbjct: 265 PSSTRVPTCWLSW 303
>gb|CO516213.1|CO516213 s13dSG67G0800068_445754 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 477
Score = 23.1 bits (44), Expect = 9.4
Identities = 7/13 (53%), Positives = 9/13 (69%)
Frame = +2 / +3
Query: 167 PTSLPLPTCMYSW 205
P+S +PTC SW
Sbjct: 327 PSSTRVPTCWLSW 365
>gb|CO516827.1|CO516827 s13dSG98C0200006_446982 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 417
Score = 23.1 bits (44), Expect = 9.4
Identities = 9/17 (52%), Positives = 10/17 (58%)
Frame = -2 / +2
Query: 60 MLSCYYTTIGFYFSTCP 10
MLSC+Y G ST P
Sbjct: 173 MLSCHYIQKGILLSTPP 223
>gb|U41472.1|MSU41472 Medicago sativa pectate lyase homolog gene, complete cds
Length = 3824
Score = 23.1 bits (44), Expect = 9.4
Identities = 6/10 (60%), Positives = 8/10 (80%)
Frame = -1 / +3
Query: 133 SKWQWRTNAE 104
SKWQWR+ +
Sbjct: 3141 SKWQWRSEGD 3170
>emb|X13287.1|MSNOD25 Medicago sativa nodulin-25 gene
Length = 10048
Score = 23.1 bits (44), Expect = 9.4
Identities = 10/19 (52%), Positives = 10/19 (52%)
Frame = -2 / -3
Query: 75 FDFFRMLSCYYTTIGFYFS 19
F FF L TIG YFS
Sbjct: 3476 FIFFTKLRALIFTIGIYFS 3420
>emb|X70707.1|MSCDC2 M.sativa mRNA for CDC2 kinase
Length = 1308
Score = 23.1 bits (44), Expect = 9.4
Identities = 11/41 (26%), Positives = 19/41 (46%)
Frame = +1 / -2
Query: 37 CCVVTRQHPEEIKAMT*PVDVTAQRLFAIAICYLCLKQRYL 159
CC+ ++ I + P ++R+F AI L + R L
Sbjct: 248 CCISFKREISRIAVLGTPSSSCSRRIFFKAIVSLVARSRAL 126
>gb|AF498987.1| Medicago sativa nodule-specific glycine-rich protein 2A mRNA,
partial cds
Length = 476
Score = 21.7 bits (41), Expect(2) = 9.7
Identities = 6/12 (50%), Positives = 8/12 (66%)
Frame = -2 / -1
Query: 45 YTTIGFYFSTCP 10
+TT+ FY CP
Sbjct: 167 FTTLSFYSMACP 132
Score = 16.2 bits (29), Expect(2) = 9.7
Identities = 5/18 (27%), Positives = 10/18 (55%)
Frame = -1 / -1
Query: 235 FHIARRQCYSP*IHASWQ 182
F + + Y +H+SW+
Sbjct: 467 FFLE*NRVYGFILHSSWE 414
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: May 2, 2006 2:40 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 2,353,331
Number of Sequences: 7669
Number of extensions: 27451
Number of successful extensions: 3841
Number of sequences better than 10.0: 36
length of database: 1,248,568
effective HSP length: 38
effective length of database: 957,146
effective search space used: 41157278
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)